Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 478,738 C→A G301V (GGT→GTT)  JCVSYN2_02210 ← DNA‑directed RNA polymerase subunit beta'

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816478,7380CA99.4% 6860.5 / NA 2291G301V (GGT→GTT) JCVSYN2_02210DNA‑directed RNA polymerase subunit beta'
Reads supporting (aligned to +/- strand):  ref base C (2/3);  major base A (1183/1087);  minor base T (5/9);  total (1191/1100)
Fisher's exact test for biased strand distribution p-value = 2.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 2345 total aligned reads displayed.

TGTAACATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTACCACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTCTAAACGTTTTAATAATTT  >  CP016816/478612‑478865
                                                                                                                              |                                                                                                                               
 gTAACATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTaaca                                                                                                                               >  1:2050831/1‑128 (MQ=255)
   aaCATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTaacacc                                                                                                                             >  2:1913236/1‑128 (MQ=255)
     cATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGATGTAGTAAATCTAACACCAt                                                                                                                           >  1:3176860/1‑128 (MQ=255)
     cATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCAt                                                                                                                           >  1:3077604/1‑128 (MQ=255)
     cATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCAt                                                                                                                           <  2:1397400/128‑1 (MQ=255)
      aTAAGTTTTTAATTATTTATAATAATTGATTGTGATACCTTTTTTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTATTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAAAACCATc                                                                                                                          <  2:5243582/128‑1 (MQ=255)
        aCGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTa                                                                                                                        <  1:4832693/128‑1 (MQ=255)
         cGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTaa                                                                                                                       >  2:4783541/1‑128 (MQ=255)
         cGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTaa                                                                                                                       >  1:3963858/1‑128 (MQ=255)
          gTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAAt                                                                                                                      >  2:2529346/1‑128 (MQ=255)
            ttttCATTATTTACAATAATTGATGGAGCAGCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTTATTTCAGAAGTAGTAAATCTAACACCATCTAATTg                                                                                                                    <  2:2322707/128‑1 (MQ=255)
             tttCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGa                                                                                                                   >  1:752874/1‑128 (MQ=255)
                    attTAGAATAATTTATGGTTCACACATTTCTTTAAATTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATATATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTg                                                                                                            <  1:1929246/128‑1 (MQ=255)
                    attTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTg                                                                                                            <  2:4698409/128‑1 (MQ=255)
                     tttACAATAATTGATGGTGCACCCATTTCTTTAACGTTTTTTAATCTTTCATTTCTAATAATGATTCTACTATATATATCATTAATTTCAGAAGTAGTAAATCAAACACCATCTAATTGaataatatg                                                                                                           >  2:3501618/1‑125 (MQ=255)
                        aCAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACg                                                                                                        <  1:92390/128‑1 (MQ=255)
                          aataatTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACCCCATCTAATTGAATAATTGGACGaa                                                                                                      >  2:2219287/1‑128 (MQ=255)
                           ataatTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATAATTAATTTCTGAAGTAGTAAATCTAACACATTCTAATTGAATTATTGTAAGAAt                                                                                                     >  1:2399625/1‑128 (MQ=255)
                             aatTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGt                                                                                                   >  1:2331104/1‑128 (MQ=255)
                                tGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCtg                                                                                                <  1:1472371/128‑1 (MQ=255)
                                 gATGGTGCACCCATTACTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAAATGTACGAATGTCtgg                                                                                               >  1:5110876/1‑128 (MQ=255)
                                  aTGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCtggt                                                                                              >  2:304461/1‑128 (MQ=255)
                                   tGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCtggtg                                                                                             >  2:1187591/1‑128 (MQ=255)
                                   tGGTGCACACATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCtggtg                                                                                             <  1:2167619/128‑1 (MQ=255)
                                    ggTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCtggtgg                                                                                            >  1:2487921/1‑128 (MQ=255)
                                     gTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGa                                                                                           <  2:915619/128‑1 (MQ=255)
                                        cACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATa                                                                                        >  2:3158266/1‑128 (MQ=255)
                                        cACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTACTATATAAAACATTAATTTCATAAGTAGTAAATCTAAAACCATCTAATTGAATAATTGGACGAATGTCTGGAGGAGTa                                                                                        >  2:3615662/1‑128 (MQ=255)
                                         tacaaTTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCATCTATATAAATCCTTAATTTCCGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATaa                                                                                       <  2:3806380/124‑1 (MQ=255)
                                         acacATTTCTTTAACATTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATaa                                                                                       <  2:5432643/125‑1 (MQ=255)
                                         aCCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATaa                                                                                       >  2:3688957/1‑128 (MQ=255)
                                          cccATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAAc                                                                                      >  2:53146/1‑128 (MQ=255)
                                           ccATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACt                                                                                     <  2:2747655/128‑1 (MQ=255)
                                             aTTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGCCGAATGTCTGGTGGAATAACTgt                                                                                   >  2:684850/1‑127 (MQ=255)
                                             aTTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTgg                                                                                   >  2:4159744/1‑128 (MQ=255)
                                              tttatttaACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGa                                                                                  <  2:1290437/128‑1 (MQ=255)
                                              tttCTTTAACTTTTTTTAATCATTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGa                                                                                  <  1:426039/128‑1 (MQ=255)
                                                  tttAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTa                                                                              <  1:2910422/128‑1 (MQ=255)
                                                   ttAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGTACGAATGTCTGGAGGAATAACTGGAATTAc                                                                             >  1:3638247/1‑128 (MQ=255)
                                                   ttAAATTTTTTTAATCTTTCAATTCTAATAATGGTGCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTAc                                                                             <  2:2124526/128‑1 (MQ=255)
                                                     aaCTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTActc                                                                           >  1:1083116/1‑128 (MQ=255)
                                                      aCTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTActct                                                                          >  2:4628177/1‑128 (MQ=255)
                                                       cTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACtctc                                                                         <  1:5007422/128‑1 (MQ=255)
                                                        tttttttAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCa                                                                        <  1:4159744/128‑1 (MQ=255)
                                                        tttttttAATCTTTCATTTCTAATAATGATTCTGCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGTAATTACTCTCa                                                                        <  1:5384618/128‑1 (MQ=255)
                                                        ttttattAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCa                                                                        <  2:4366612/128‑1 (MQ=255)
                                                          tttttAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTTGAATAACTGGAATTACTCTCATa                                                                      >  1:3413523/1‑128 (MQ=255)
                                                                aTCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACcatt                                                                <  1:4941218/128‑1 (MQ=255)
                                                                 tCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACcattc                                                               <  2:607584/128‑1 (MQ=255)
                                                                    ttCATTTCTTCTACTGATTCTTCTATATAAATCATTTATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACcattcatt                                                            <  2:5171986/128‑1 (MQ=255)
                                                                     tCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCattcattc                                                           >  1:831898/1‑128 (MQ=255)
                                                                     tCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCattaattc                                                           >  1:1841508/1‑128 (MQ=255)
                                                                     tCATTTCTAATAATGATTCATCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCattcattc                                                           <  1:4592207/128‑1 (MQ=255)
                                                                      cATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCAttcattca                                                          >  1:4667493/1‑128 (MQ=255)
                                                                           cTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACg                                                     <  2:4495467/128‑1 (MQ=255)
                                                                           cTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAACAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACg                                                     <  2:2021123/128‑1 (MQ=255)
                                                                            taataaTGGTTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGt                                                    <  2:242771/128‑1 (MQ=255)
                                                                              ataatGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTg                                                  >  1:4479292/1‑128 (MQ=255)
                                                                               taatGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTgg                                                 <  1:436968/128‑1 (MQ=255)
                                                                               taatGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTgg                                                 <  1:1717022/128‑1 (MQ=255)
                                                                                aatGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGt                                                <  2:1475553/128‑1 (MQ=255)
                                                                                aatGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGt                                                <  2:5532141/128‑1 (MQ=255)
                                                                                aatGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGt                                                <  2:393074/128‑1 (MQ=255)
                                                                                 atGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGtt                                               <  1:5519812/128‑1 (MQ=255)
                                                                                   gATTCTTCTCAATACATGAATAAATTCCGAAGTAGTAAACCTAACACCATCTAATTGAATAATGGGACGAATGTCTGGTTGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAg                                             <  2:1106213/128‑1 (MQ=255)
                                                                                     ttcttcTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCAACTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGaa                                           <  1:639414/128‑1 (MQ=255)
                                                                                      tcttctATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAAc                                          >  1:1381827/1‑128 (MQ=255)
                                                                                        ttctATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGt                                        >  2:3261034/1‑128 (MQ=255)
                                                                                         tctATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGtt                                       <  1:1599596/128‑1 (MQ=255)
                                                                                             actaAATCATTAATTTCAGAAGAAGTAACTCTAACACCATCTAATTGAATAATTGGACGAATGGCTGGTGGAATAACTGGAATTCCTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTaa                                   <  2:338823/126‑1 (MQ=255)
                                                                                              ataCATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTaaa                                  <  1:4756883/128‑1 (MQ=255)
                                                                                                aaaTCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGc                                <  2:809588/128‑1 (MQ=255)
                                                                                                 aaTCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCa                               >  2:4258280/1‑128 (MQ=255)
                                                                                                    cATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATCTCTGCTGGAATAACTCGAAATACTCTCAGAACCATTCATTCACGACGTTGGATAGAACGTTTTAAAGCATCa                            >  1:1002651/1‑128 (MQ=255)
                                                                                                       tAATTTCAGAAGTAGTAAATCTAACACCATCTATTTGAATAATTTGAAGAATGTCTGGTGTAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCaaaa                         <  1:1286322/128‑1 (MQ=255)
                                                                                                       tAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCaaaa                         <  1:5270526/128‑1 (MQ=255)
                                                                                                       tAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCaaaa                         <  2:1812516/128‑1 (MQ=255)
                                                                                                           ttCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAATGCATCAAAAGttt                     >  2:4056054/1‑128 (MQ=255)
                                                                                                           ttCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTATTAAAGCATCAAAAGttt                     >  1:3779308/1‑128 (MQ=255)
                                                                                                            tCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGCTAAGAACGTTTTAAAGCCTCAAACGttta                    >  2:5085989/1‑127 (MQ=255)
                                                                                                             cAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTCt                   <  2:2626620/128‑1 (MQ=255)
                                                                                                              aGAATTAGTAAATCTATCTCCATCTAATTGAATAATTGGACGAATGTCGGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTCTa                  <  2:2856610/128‑1 (MQ=255)
                                                                                                              aGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACTTTTTAAAGCATCAAAAGTTTCTa                  >  2:1030060/1‑128 (MQ=255)
                                                                                                              aGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTCTa                  >  1:3522275/1‑128 (MQ=255)
                                                                                                               gAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTCTaa                 <  1:2706863/128‑1 (MQ=255)
                                                                                                               gAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTCTaa                 >  1:3006149/1‑128 (MQ=255)
                                                                                                                   tagtaAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGATTTACTGGAATTACTCTCATAACCATTCATTCACGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTCTAAActt             >  2:1641495/1‑126 (MQ=255)
                                                                                                                   tagtaAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTCTAAACGt             >  1:2586667/1‑128 (MQ=255)
                                                                                                                   tagtaAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTCAAAACGt             >  2:295275/1‑128 (MQ=255)
                                                                                                                      taAATCTAACACCATCTAATTGAATAATTGGACGATTGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTCTAAACGtttt          <  2:4880891/128‑1 (MQ=255)
                                                                                                                      taAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTTAAGCATCAAAAGTTTCTAAACGtttt          >  2:4981745/1‑128 (MQ=255)
                                                                                                                      taAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTCTAAACGtttt          >  1:3310667/1‑128 (MQ=255)
                                                                                                                      taAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTCTAAACGtttt          <  2:1556359/128‑1 (MQ=255)
                                                                                                                      taAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACAATTCATTCAGGACTTTGGATAGAACGTTTTAAAGCATCAAAAGTATCTAATCGtttt          >  1:519056/1‑128 (MQ=255)
                                                                                                                       aaaTCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTaa                                   >  1:4057374/1‑102 (MQ=255)
                                                                                                                       aaaTCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTCTAAACGTTTta         >  1:1190059/1‑128 (MQ=255)
                                                                                                                        taTCTAAAACAATATAATTGTATAATTTGAAGAATGTATGGTTGAATAACTGGAATTACTATCATAACCTTTAATTAAGTACGTTGGTTAGAACGTTTTTAAGCATCAAAAGTTTCTAATCGTTTtaa        <  2:1750814/127‑1 (MQ=255)
                                                                                                                        aaTCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGATCGTTTTAAAGCATCAAAAGTTTCTAAACGTTTtaa        <  1:3864809/128‑1 (MQ=255)
                                                                                                                        aaTCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTCTAAACGTTTtaa        >  1:282048/1‑128 (MQ=255)
                                                                                                                          tCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTCTAAACGTTTtaata      >  2:3984421/1‑128 (MQ=255)
                                                                                                                              |                                                                                                                               
TGTAACATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTACCACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTCTAAACGTTTTAATAATTT  >  CP016816/478612‑478865

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑