BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP016816	281,616	C→T	Q100* (CAA→TAA)	JCVSYN2_01185 →	DNA‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP016816	281,616	0	C	T	99.7%	10256.5 / NA	3231	Q100* (CAA→TAA)	JCVSYN2_01185	DNA‑binding protein
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (1606/1611); minor base A (4/7); total (1612/1619)
Fisher's exact test for biased strand distribution p-value = 5.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 3254 total aligned reads displayed.

ATGATTCTATTTCTAAAACTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAACAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATATTGTTGATCAAAATCAAATA  >  CP016816/281491‑281740

aTGATTCTATTTCTAAAACTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATaa                                                                                                                            <  1:676459/128‑1 (MQ=255)

 tGATTCTATTTCTAAAACTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAaataat                                                                                                                           <  1:4708622/128‑1 (MQ=255)

       tATTTCTAAAACTAGTAAATTATTATTTAATTTAGAGACAAAATTATATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATaa                                                                                                                     <  1:2442427/128‑1 (MQ=255)

       tATTTCTAAAACTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATaa                                                                                                                     <  2:6860135/128‑1 (MQ=255)

           tCTAAAACTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAATATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAg                                                                                                                 >  1:2093588/1‑128 (MQ=255)

           tCTAAAACTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAg                                                                                                                 <  2:1559380/128‑1 (MQ=255)

               aaaCTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTa                                                                                                             <  1:1833486/128‑1 (MQ=255)

                aaCTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGtt                                                                                                               <  1:1007442/125‑1 (MQ=255)

                 aCTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTaaa                                                                                                           <  1:6301166/128‑1 (MQ=255)

                 aCTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTaaa                                                                                                           >  2:13306/1‑128 (MQ=255)

                   tAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAag                                                                                                         >  1:3258529/1‑128 (MQ=255)

                   tAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAag                                                                                                         >  1:5075056/1‑128 (MQ=255)

                   tAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAag                                                                                                         >  1:1130704/1‑128 (MQ=255)

                     gTAAATTATTATTTAATTTTGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAagag                                                                                                       <  2:6626971/128‑1 (MQ=255)

                     gTAAATTATTATTTAATTTAGAGTCAAAAATTCATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAagag                                                                                                       <  2:394071/128‑1 (MQ=255)

                     gTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAagag                                                                                                       <  2:4071308/128‑1 (MQ=255)

                     gTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAagag                                                                                                       >  1:835956/1‑128 (MQ=255)

                      tAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTtata                                                                                                                      >  2:1768107/1‑112 (MQ=255)

                      tAAATTATTATTTAATTTAGAGACAAAATTACATATAAAACAAAAACAGGATTTACTTATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAAAATATAAAAATTTTAAAAGAgg                                                                                                      >  2:4688381/1‑128 (MQ=255)

                           tattattTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGAtt                                                                                                 >  2:4987199/1‑128 (MQ=255)

                            attattTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGAttt                                                                                                <  2:834062/128‑1 (MQ=255)

                            attattTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGAttt                                                                                                <  1:4193966/128‑1 (MQ=255)

                            attattTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGAttt                                                                                                >  1:3906366/1‑128 (MQ=255)

                              tattTAATTTAGAGACAAAATTACATTTAAAAAAAAATCAGGATTTACTAATTAATCTAATTAATAAAATAGAAAAAAAAAATATAGATTAAAATTTATTTATAAATATTTTAATAGAGGTTAtttta                                                                                              >  2:3474209/1‑126 (MQ=255)

                                 ttaatttaGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGATACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTtgatga                                                                                           <  1:704784/128‑1 (MQ=255)

                                 ttaaattaGAGACAAAATTAAATTTAAAACAAACAAAGGATTTACTAATTTCTCTAATAAATAAAATTGAAACAAATCAAATAGATGAAAAATCATTTATAAAAAGTTTAAAAGAGGTGATTtgatga                                                                                           <  2:4435218/128‑1 (MQ=255)

                                    atttaGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGGGGTGATTTGATGAAAg                                                                                        <  1:4315810/128‑1 (MQ=255)

                                    atttaGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGTTGATTTGATGAAAg                                                                                        >  1:2582366/1‑128 (MQ=255)

                                    atttaGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAg                                                                                        <  1:4455155/128‑1 (MQ=255)

                                    atttaGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATATATAAAAAGTTTAAAAGAGTTGATTTGATGATAg                                                                                        >  1:362817/1‑128 (MQ=255)

                                     tttaGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGt                                                                                       <  1:2999667/128‑1 (MQ=255)

                                         gagaCAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTa                                                                                   <  1:3372523/128‑1 (MQ=255)

                                           gaCAAAATTACATTTAAAACAAAAACATGATTTACTAATTTCTCTAATTAATAAATTTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAAt                                                                                 <  1:2213209/128‑1 (MQ=255)

                                             cAAAATTACATTTAAAACAAAAAAAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGa                                                                               <  1:5418739/128‑1 (MQ=255)

                                             cAAAATTAAATTTAAAACAAAAACAGGATTTACTAATTTCTATAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGa                                                                               <  2:5197432/128‑1 (MQ=255)

                                              aaaaTTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGAt                                                                              >  1:1742894/1‑128 (MQ=255)

                                              aaaaTTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGAGTTGATGAAAGTATTAATGAt                                                                              >  2:2822775/1‑128 (MQ=255)

                                                 aTTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTAGTAATGAACgg                                                                           >  2:1117283/1‑128 (MQ=255)

                                                   tACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAATATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGgag                                                                         <  2:2354523/128‑1 (MQ=255)

                                                   tACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGgag                                                                         >  1:4927072/1‑128 (MQ=255)

                                                    aCATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGgaga                                                                        <  2:2693896/128‑1 (MQ=255)

                                                    aCATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGgaga                                                                        >  1:2326939/1‑128 (MQ=255)

                                                      aTTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGttt                                                                                                              >  1:5277194/1‑88 (MQ=255)

                                                       tttAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGTAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGt                                                                     <  2:6271625/128‑1 (MQ=255)

                                                        ttAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGtt                                                                    <  2:198404/128‑1 (MQ=255)

                                                         tAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTATAAAATAGGTGATTTCATGAAAGTATTAATGATCGGAGATGttt                                                                   >  2:1686259/1‑128 (MQ=255)

                                                          aaaccaaaaacaGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTa                                                                  <  2:2990369/128‑1 (MQ=255)

                                                          aaaacaaaaacaGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATg                                                                                <  2:5391831/114‑1 (MQ=255)

                                                          aaaacaaaaacaGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGttga                                                                  >  2:5498888/1‑126 (MQ=255)

                                                          aaaacaaaaacaGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTa                                                                  <  1:3121142/128‑1 (MQ=255)

                                                          aaaacaaaaacaGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTa                                                                  >  1:4603707/1‑128 (MQ=255)

                                                           aaacaaaaacaGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTAt                                                                 <  1:4051975/128‑1 (MQ=255)

                                                            aacaaaaacaGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATATAAAGTTTAAAAGAGGTGATTTTATGATAGTATTATTGATCGTATATGTTTATg                                                                >  2:5796845/1‑128 (MQ=255)

                                                            aacaaaaacaGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTATAAAAGAGGTGATTTGATGAAAGTATTAATTATCGGAAATTTTGATg                                                                >  2:2265535/1‑128 (MQ=255)

                                                             acaaaaacaGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTATTGATCGGAGATGTTTATGc                                                               >  2:4567522/1‑128 (MQ=255)

                                                                aaaacaGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCaac                                                            >  2:6712350/1‑127 (MQ=255)

                                                                aaaacaGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGGATTAATGATCGGAGATGTTTATGCaaa                                                            <  1:1521679/128‑1 (MQ=255)

                                                                     aGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCgg                                                       >  1:482115/1‑128 (MQ=255)

                                                                     aGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCgg                                                       >  1:5761214/1‑128 (MQ=255)

                                                                     aGGATTTACTAATTTCTCTAAATAATAAACTTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCgg                                                       <  2:4327143/128‑1 (MQ=255)

                                                                      ggATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAATTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAATCAggg                                                      >  1:6423783/1‑128 (MQ=255)

                                                                        aTTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGaa                                                    <  1:618239/128‑1 (MQ=255)

                                                                          ttACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAaga                                                  >  1:3812006/1‑128 (MQ=255)

                                                                            aCTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAgaga                                                <  2:1460741/128‑1 (MQ=255)

                                                                            aCTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAgaga                                                >  1:1622186/1‑128 (MQ=255)

                                                                             cTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGt                                                                     <  1:1241380/106‑1 (MQ=255)

                                                                             cTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGt                                                                     >  2:1241380/1‑106 (MQ=255)

                                                                             cTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGaa                                               >  1:1973613/1‑128 (MQ=255)

                                                                                aTTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGACAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATg                                            <  1:1358430/128‑1 (MQ=255)

                                                                                 tttCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGc                                           >  1:6019133/1‑128 (MQ=255)

                                                                                     tctAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGa                                       <  2:5090137/128‑1 (MQ=255)

                                                                                      ctAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGCTCAGAGATTTATATGCAAAACCGGGAATATAAATGCTAGaa                                      >  2:1034077/1‑128 (MQ=255)

                                                                                        aattaatAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGaaaa                                    <  1:6646877/128‑1 (MQ=255)

                                                                                           taataaAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACa                                 >  2:3827686/1‑128 (MQ=255)

                                                                                            aataaAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACAt                                >  1:5526419/1‑128 (MQ=255)

                                                                                             ataaAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACAtt                               >  2:3345446/1‑128 (MQ=255)

                                                                                              taaAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGTAGAGATATGCTAGTAAAACAtgt                              >  2:1881048/1‑126 (MQ=255)

                                                                                               aaaaTTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTa                             >  2:4218741/1‑128 (MQ=255)

                                                                                                 aaTTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTaaa                           <  2:2574566/128‑1 (MQ=255)

                                                                                                  aTTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTaaaa                          <  1:5879051/128‑1 (MQ=255)

                                                                                                   ttGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGAAGAAAGTATTAATGATCGGAGCTGTTTATGCAAAACCGGGAAGAGAAATGCTAGCAAAACAGTTaaata                         >  1:3517885/1‑126 (MQ=255)

                                                                                                    tGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTaaaaaa                        <  2:6400994/128‑1 (MQ=255)

                                                                                                    tGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTaaaaaa                        <  2:3097783/128‑1 (MQ=255)

                                                                                                     gcaccaaatcaaatAGAGGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAat                       <  2:3589593/124‑1 (MQ=255)

                                                                                                     gAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCgg                                                       >  1:2741211/1‑96 (MQ=255)

                                                                                                     gAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGTAGAGAAATGCTAGAAAAACATTTAAAAAat                       >  2:6040040/1‑128 (MQ=255)

                                                                                                     gAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAat                       >  2:6504432/1‑128 (MQ=255)

                                                                                                     gAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTAATAATGATCGGAGATG‑TTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAata                      <  1:5151190/128‑1 (MQ=255)

                                                                                                          aaatcaaatAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATAttgt                  <  2:6169029/128‑1 (MQ=255)

                                                                                                            atcaaatAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAACACATTTAAAAAATAttgttg                >  2:3731815/1‑128 (MQ=255)

                                                                                                            atcaaatAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAAACGGGAAGAGAAATGCTAGTAAAACATTTAAAAAATAttgttg                >  2:2463201/1‑128 (MQ=255)

                                                                                                            ataatatAGATGAAAAATAATATATAAAATGTTTAAAAGAGGGGATTTGATGATAGTATTATAGTTCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATAttgttg                <  2:2121892/123‑1 (MQ=255)

                                                                                                             tcaaatAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATATTGTTGa               >  2:4831344/1‑128 (MQ=255)

                                                                                                              caaatAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATATTGTTGat              <  2:5890086/128‑1 (MQ=255)

                                                                                                               aaatAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATATTGTTGatc             <  1:6541821/128‑1 (MQ=255)

                                                                                                                 atAGATTAAAAATAATTTATAAAAAGTTTAAAAGATGTGATTTGTTGATAGTATTAATGATCCGATATGTTTATGCAAAACCGTGAAGAGAAATGCTATAAAAACATTTAAAAAATATTGTTTttcaa           <  2:5285024/128‑1 (MQ=255)

                                                                                                                    gATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAATACCGGGAAGAGAAATGCTAGAAATACATTTAAAAAATATTGTTGatcaaaat        >  2:3224450/1‑128 (MQ=255)

                                                                                                                    gATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATATTGTTGatcaaaat        >  1:5670477/1‑128 (MQ=255)

                                                                                                                        aaaaaTACTTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATATTGTTGatcaaaatcaaa    <  1:2876381/128‑1 (MQ=255)

                                                                                                                         aaaaTAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATATTGTTGATCAAAATCAAAt   >  2:5646825/1‑128 (MQ=255)

ATGATTCTATTTCTAAAACTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAACAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATATTGTTGATCAAAATCAAATA  >  CP016816/281491‑281740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`