Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816202,4850T.51.9% 370.4 / inf 479intergenic (+263/‑412)JCVSYN2_00840/JCVSYN2_00845hypothetical protein/lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (36/170);  new base . (124/98);  total (179/300)
Fisher's exact test for biased strand distribution p-value = 8.62e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.49e-04

Only 100 of 543 total aligned reads displayed.

AAGTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCCTTTTAAAAAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAATATATATCTAATATATA  >  CP016816/202358‑202606
                                                                                                                                   |                                                                                                                         
aaGTTAGTTTAAATAATAGAGTTAATGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCCt                                                                                                                           <  1:1762067/128‑1 (MQ=255)
aaGTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCCt                                                                                                                           <  1:3811558/128‑1 (MQ=255)
aaGTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCCt                                                                                                                           <  1:3673735/128‑1 (MQ=255)
 agttagttTAATTAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCCtt                                                                                                                          <  1:1428685/128‑1 (MQ=255)
 aGTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCCtt                                                                                                                          <  2:2465028/128‑1 (MQ=255)
 aGTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAAAAAACCCAtt                                                                                                                          >  1:3972404/1‑128 (MQ=255)
  tttAGTGTAAATAATAGAGTTAATGTTAATTAAAATACCATAAAATTAATAATTCTTATAATTTAGATCCTCGTGATCTATTTTTTTATTTTTTTATCTTCACAAATAAAAAAAAAACAATCCCCtta                                                                                                                         <  2:2725405/127‑2 (MQ=255)
  gTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCCtta                                                                                                                         >  1:3776423/1‑127 (MQ=255)
   ttAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCCtaaa                                                                                                                        >  1:463202/1‑125 (MQ=255)
   ttAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAAAAATCCCCtaaa                                                                                                                        >  2:3456267/1‑125 (MQ=255)
     attgtAAATAAAAGAGTTAAGGTTAATTAAAATAACATAAACTTAATAATTCTTATAATGTATATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCCttaaaa                                                                                                                      <  1:745239/126‑5 (MQ=255)
      gtgtAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTAATTTGTTTATCTTCACAAATAAAAAAAAAAAAATCACCtaaaaaa                                                                                                                     >  1:4019490/1‑122 (MQ=255)
       tgtAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAACAATCCCCttaaaaata                                                                                                                   >  1:3863528/1‑121 (MQ=255)
       tgtAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTATATATTCACAAATAAAAAAAAAAACAATACCCtaaaaaa                                                                                                                     >  1:3561960/1‑122 (MQ=255)
        gtAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCACttaaaaata                                                                                                                   >  1:4046007/1‑121 (MQ=255)
          aaaTAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCCt                                                                                                                           >  1:2415200/1‑118 (MQ=255)
             taataGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTTTTTATCTTCACAAATAAAAAAAAAAAAAAAACCATtaaaaaataaaa                                                                                                             >  1:1550804/1‑128 (MQ=255)
              aataGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCC‑‑‑TAAAAAATtaaaaaa                                                                                                          >  1:3537642/1‑128 (MQ=255)
                taGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAAACAAACCCCTT‑‑‑AAAAATaaaaaaaa                                                                                                         >  1:4192471/1‑128 (MQ=255)
                taGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAAAAAAACC‑‑AATAAAAAATaaaaaaaa                                                                                                         >  1:2870645/1‑128 (MQ=255)
                taGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTATTATAATATATATCCTCTTGATCTAAATAAATATTTGATTATCTTCACAAATAAAAAAAAAACAATCAAC‑‑‑TAAAAAATaaaaaaaaa                                                                                                        >  2:1781205/1‑128 (MQ=255)
                 agagTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTTTTTATCTTCACAAATAAAAAAAAAACAAACCAC‑‑TTAAAAATTaaaaaaaaa                                                                                                        >  2:3722874/1‑128 (MQ=255)
                    tttAAGGTTAATTAAAATACAAAAAACATAAAAATTATTATAATTTTTATCCTCGTGATCTATTTTTTTTTTTTTTATCTTCACAAAAAAAAAAAAAACAATCCCC‑‑TTAAAAATTaaaaaaaaaaaaa                                                                                                    <  2:4252121/127‑1 (MQ=255)
                    gTTAAGGTTAATTAAAATAAAATAAAATTAATAATTTTTATAATTTTGATTCTTGTGATCTTTTTTTTTATTTGTTTATAATAACAAATAAAAAAAAA‑‑AATCCCC‑‑ATAAAAATTaaaaaaaaaaaaaa                                                                                                   <  2:4146808/128‑1 (MQ=255)
                    gTTAACTTTAATTAAAATACCATAAAATTAATAATTATTATAATTTATATCCTCTTTATATATTTTTTTATTTGTTTATCATAAAAAATAAAAAAAAAACAATCCCC‑‑TTAAAAATTaaaaaaaaaaaa                                                                                                     <  1:2377948/128‑1 (MQ=255)
                     ttaattttaattaaAATAACAAAAACTTAAAAATATTTTTAAATTTTATCCACGTTATATATTTTTTTATTTGTTTATCTTCAAAAATAAAAAAAAAACAATCCCC‑‑TTAAAAATTaaaaaaaaaaaaa                                                                                                    <  2:3492774/122‑1 (MQ=255)
                     ttAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCTTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAAACCCATT‑‑‑AAAAATaaaaaaaaaaaaaa                                                                                                   >  1:944261/1‑128 (MQ=255)
                     ttAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAAACCCC‑‑TTAAAAATTaaaaaaaaaaaaa                                                                                                    >  1:4089465/1‑128 (MQ=255)
                     ttAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCATCGTGATCTATATATTTATTAATTTATCTTCACAAATAAAAAAAAAAAAAAACCC‑‑‑TAAAAAATaaaaaaaaaaaaaa                                                                                                   >  2:2566026/1‑128 (MQ=255)
                      tAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAACAAATCCC‑‑‑ATAAAAAATtaaaaaaaaaaaaaa                                                                                                  >  2:4046245/1‑128 (MQ=255)
                      tAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAACAATAACC‑‑‑TAAAAAATaaaaaaaaaaaaaaaa                                                                                                 >  1:2993178/1‑128 (MQ=255)
                       aaGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAAAAATCACCTT‑‑‑AAAAATaaaaaaaaaaaaaaaa                                                                                                 >  2:4008646/1‑128 (MQ=255)
                       aaGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTATTATTTTATTATCTTCACAAATAAAAAAAAAACAATCAC‑‑‑ATAAAAAATtaaaaaaaaaaaaaaa                                                                                                 >  1:2005740/1‑128 (MQ=255)
                        atgTTAATTAAAATACAATAAAATTAATAATTTTATAAAATTATATACTCTTTATATATTTTTTTATTTTTTTATATTAACAAAAAAAAAAAAAAAAATCCCC‑‑TTAAAAATTaaaaaaaaaaaaaaaa                                                                                                 <  2:4392112/126‑1 (MQ=255)
                        aGGTTAATTAAAAAACCATAAAATTAATAATTATAATAATGTATATACTCGTGATCTATTTTTTTAATTTTTTATATTCACAAATAAAAAAAAAACAAACCCC‑‑TTAAAAATTaaaaaaaaaaaaaaaa                                                                                                 <  2:328096/128‑1 (MQ=255)
                         ggTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAAACCCC‑‑‑TAAAAAATaaaaaaaaaaaaaaaaaa                                                                                               >  1:4392112/1‑128 (MQ=255)
                         ggTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATATTCACAAATAAAAAAAAAACAATCCC‑‑ATTAAAAAATaaaaaaaaaaaaaaaaa                                                                                                >  1:3420143/1‑128 (MQ=255)
                            taattaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCC‑‑‑TAAAAAATtaaaaaaaaaaaaaaaaaaaa                                                                                            >  1:3462531/1‑128 (MQ=255)
                            taattaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCC‑‑ATTAAAAATTaaaaaaaaaaaaaaaaaaaa                                                                                             >  2:3562647/1‑128 (MQ=255)
                            taaataaaAAAACAAAAAATTAAAAATTTTTATAATTTATAAACACGTTATATATTTTTTTATTTTTTTATAATAAAAAAAAAAAAAAAAAAAAACCCC‑‑TTAAAAATTaaaaaaaaaaaaaaaaaaaa                                                                                             <  2:3955937/128‑1 (MQ=255)
                             aattaaAATACCATAAACTTAATAATTCTTATAATGTATATCATCGTGATATATTTTTATATTAGAATATCTTCACAAATAAAAAAAAAACAAT‑‑CAAAAAAAAAAATaaaaaaaaaaaaaaaaaaaaa                                                                                            >  2:1012030/1‑128 (MQ=255)
                             aattaaAATAACATAAAATTAAAAATTTTTATAATTTATTTACTCGTTATATATTTTTTTATTTGTTTATATTAAAAAAAAAAAAAAAAAACAATCCCC‑‑TTAAAAATTaaaaaaaaaaaaaaaaaaac                                                                                             <  2:3657533/128‑2 (MQ=255)
                             aattaaAATAACAAAAAATTTATATTTTTTTTAATTTTTATTCTCTTTATTTATTTTTTTATTTTTTTTTATTAAAAAATAAAAAAAAAAAAAACCCC‑‑TTAAAAATTaaaaaaaaaaaaaaaaaaaac                                                                                            <  1:4156803/128‑2 (MQ=255)
                               ttaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTATTTATCTTCACAAATAAAAAAAAAACAAT‑‑‑AAAATAAAAAATaaaaaaaaaaaaaaaaaaaaataa                                                                                         >  2:4473953/1‑125 (MQ=255)
                                taaAATACCATAAACTTAATAATTCTTATAATGTAGATCATCGTGATCTATTTTTTTATTTGTTTATCTTAACAAATAAAAAAAAAACAAT‑‑AAAATAAAAAATTaaaaaaaaaaaaaaaaaaaaaaaa                                                                                         >  1:132810/1‑125 (MQ=255)
                                  aaaTACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCC‑‑TTAAAAATTaaaaaaaaaaaaaaaaaaaaaaaaaa                                                                                       >  1:1243959/1‑123 (MQ=255)
                                  aaaTACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAAAAATCAC‑‑ATTAAAAATTaaaaaaaaaaaaaaaaaaaaaaaaaa                                                                                       >  2:548283/1‑123 (MQ=255)
                                  aaaTACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAAAAATAAAC‑‑TAAAAAATTaaaaaaaaaaaaaaaaaaaaaaaaaa                                                                                       >  1:1616926/1‑123 (MQ=255)
                                   aaaaaaaaaaaaTTAATAATTATTATAATTTATATCCTCGTGATAAATTTTTTTATTTGTTTATATTAACAAATAAAAAAAAAAAAAACCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAACTAAaaac                                                                                    <  1:172521/120‑1 (MQ=255)
                                   aaTACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCC‑‑TTAAAAATTAaaaaaaaaaaaaaaaaaaaaaaaaaa                                                                                      >  1:546796/1‑128 (MQ=255)
                                    aatacATAAAATTAATAATTATTATAAATTATTTACAAGTGATTAATTTTTTTTTTTTTTTAAATTAAAAAATAAAAAAAAAAAAATCCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAACTAAaaaca                                                                                   <  2:1113819/124‑1 (MQ=255)
                                     atcaaTAAAATTAATAAATCTTAATATGTATAACCCCGTTTTATATTTTTTTTTTTTTTTATATTAAAAAATAAAAAAAAAACAATCCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAaaaca                                                                                   <  2:3836549/124‑1 (MQ=255)
                                     aaacaaaaaaTTAATAATTTTTATAAATTTTATCATATTTATATTTTTTTTTAATTTTTTATATTAAAAAAAAAAAAAAAAAAAATCCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAACTAAaaacaa                                                                                  <  2:2104003/122‑1 (MQ=255)
                                      aacATAAAATTAATAATTATTATAATTTATATAATCGTTATTTATTTTTTTATTTTTTTATCTTAAAAAAAAAAAAAAAAACAATCCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAACTAAaaacaaa                                                                                 <  2:1594418/126‑1 (MQ=255)
                                        cATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACCATCCCC‑‑TTAAAAATTaaaaaaaaaaaaaaaaaacaaaaaccaaaact                                                                                 >  1:2341601/1‑117 (MQ=255)
                                         aataaattAAAAATTTTTAAAATTTATATATTAGTTATTAATTTTTTTTTTTTTTTATATTAAAAAATAAAAAAAAAAAAAACCCCTT‑‑‑AAAAAT‑‑TAAAAAAAAAAAAAAAAAACTAAAAACAAActct                                                                             <  2:4308767/122‑1 (MQ=255)
                                         aaaaaaTTAATAATTATTATAATTTATATCATATTTATCTATTTTTTTATTTTTTTATATTAAAAAAAAAAAAAAAAAAAATCCCC‑‑TTAAAAAAT‑‑‑AAAAAAAAAAAAAAAAAACTAAAAACAAActct                                                                             <  2:4092868/126‑1 (MQ=255)
                                         aTAAAATTAATAATTATTAAAATTAAAATACACTTTATATATTTTTTTATTTTTTTATATTAAAAAAAAAAAAAAAAACAATCCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAACTAAAAACAAActc                                                                              <  2:754566/128‑1 (MQ=255)
                                          tAAAATTAAAAATTCTAATAATGTACAACCTCGTGATCAATTTTTTTATTTGTTTAACTTAACAAATAAAAAAAAAACAATCCCCAT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAACTAAAAACAAActct                                                                             <  2:868899/128‑1 (MQ=255)
                                          tAAAATTAAAAAATATTAAAATTTATATACAAGAGATATATTTTTTTATTTTTTTAAAATAAAAAAAAAAAAAAAAAAAAACCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAActc                                                                              <  2:1421278/128‑1 (MQ=255)
                                            aaattAAAAATTATTATAATGTATATACTCGTTATATATTTTTTTATTTTTTTATTTTAAAAAATAAAAAAAAAAAAATACCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTa                                                                            <  2:1670437/125‑1 (MQ=255)
                                             tataattaatTTTTATAATTTATATTCTCGTTTTTTATTTTTTTTTTTGTTTATTTTTAAAAAAAAAAAAAAAAAAAACCCC‑‑TTAAAAATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTa                                                                            <  1:4301066/122‑1 (MQ=255)
                                               ataatatTTTTTTTAATTTAGATTCTTGTTTTTTATTTTTTTATTTTTTTATTTTTAAAAATAAAAAAAAAACAAACCCCTT‑‑‑AAAAAT‑‑TAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCt                                                                       <  1:2566026/127‑1 (MQ=255)
                                                     attattAAAATGTTTATCCTTCTGTTATATTTTTTTATTTTTTTAAAATAAAAAAAAAAAAAAAACAATACCCAT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAAt                                                                 <  2:2958548/128‑1 (MQ=255)
                                                            tttaagTAGATCATCTGTATATATTATTTTTTTTTTTAATCTTCAAAAATAAAAAAAAAACAATACCCAT‑‑‑AAAAAT‑‑‑TAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCtat                                                          <  2:2881223/123‑1 (MQ=255)
                                                                ttaatataatagTGATCTAATTTTTTATTTTTTTATATTCACAAATAAAAAAAAAAAAATCCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCtatt                                                         <  2:4191667/117‑1 (MQ=255)
                                                                 tttaaTCCTAGTGATTTTATTTTTTATTTTTTATTTTTAAAAAAAAAAAAAAAAAAAATCCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCtattat                                                       <  1:307779/124‑1 (MQ=255)
                                                                   tcttCCTCGTGATGTTTTTTTTTTTTTGTTTATATTCACAAAAAAAAAAAAAAAAATCCCCTT‑‑‑AAAAAT‑‑‑TAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACca                                                   <  1:4432224/125‑1 (MQ=255)
                                                                   atttcctccTGATCTATTTTTTTATTTTTTTATCTTCACAAATAAAAAAAAAACAATCCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATAc                                                     <  2:3789743/125‑1 (MQ=255)
                                                                     attatatttatttatttttttattttttTTTTATAATAAATTAAAAAAAAAAAATTACC‑‑TTATAAAAT‑‑AAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACca                                                   <  2:1907504/116‑1 (MQ=255)
                                                                       tatagtttttatttttttttttttttTATATTAAAAAAAAAAAAAAAAAAAATACACTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACcac                                                  <  1:1012030/116‑1 (MQ=255)
                                                                          tttttaatttttttttATTTTTTATAATAAAAAAAAAAAAAAAAAAAAAACCCATT‑‑AAAAATAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACattt                                              <  2:3021414/119‑1 (MQ=255)
                                                                          tttgattattttttatatttttttatattAAAAAATAAAATAAAAAAAATACCCATT‑‑‑‑AAATATTAAAAAAAAAAAAAAAAACTAAAAACAAAGTCTATTTCTTAAAATTCCCTATTATACCACatttt                                             <  2:3709766/119‑1 (MQ=255)
                                                                          tgtgaatattttttttAATTTTTTATTATAACAAATAAAAAAAAAAAAAAACCC‑‑ATAAAAAAT‑‑AAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACattt                                              <  2:3689263/119‑1 (MQ=255)
                                                                          tctgttaatttttttttTATTTTTTTTTTAAAAAATAAAAAAAAAAAAATACC‑‑ATTAAAAAAT‑‑AAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACattt                                              <  1:4538931/119‑1 (MQ=255)
                                                                          cgttttataattttttATTTTTTTAACATCACAAAAAAAAAAAAAAACAATCCCCTT‑‑‑AAAAAT‑‑TAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACattt                                              <  2:2285581/118‑1 (MQ=255)
                                                                              ttatatttttttattttttTATTTTAAAAAATTAAAAAAAAACAATACCCTT‑‑‑AAAAAT‑‑‑TAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACattttatttt                                        <  2:3533305/125‑1 (MQ=255)
                                                                              ttatATTTTTTTATTTGTTTATATTAAAAAAAAAAAAAAAAACAAAACACTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACattttatt                                          <  2:3648466/125‑1 (MQ=255)
                                                                                atttttttttatttttttaTATTAAAAAAAAAAAAAAAAACAATAACCTT‑‑‑AAAAAT‑‑TAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTAttttt                                       <  1:3919214/125‑1 (MQ=255)
                                                                                aattttttttatttttttaTATTAAAAAAAAAAAAAAAAAAAATACCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACattttatttt                                        <  1:1149412/125‑1 (MQ=255)
                                                                                 taatttattATTTTTTTATAATATAAAATAAAAAAAAAACAATACCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTAttttt                                       <  2:3360331/125‑1 (MQ=255)
                                                                                  tattttttATTTGTTTATATTAAAAAAAAAAAAAAAAAAAAATCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTa                                      <  1:1094247/126‑1 (MQ=255)
                                                                                  aTTTTTTTATTTGTTATTATTAAATAATAAAAAAAAAAAAATAACCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTa                                      <  2:2152654/128‑1 (MQ=255)
                                                                                   tttttttatttttttaTAATAAAAAAAAAAAAAAAAACAATCCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTa                                      <  2:580604/128‑1 (MQ=255)
                                                                                    ttttttatttttttatATTAAAAAAAAAAAAAAAAAAAATACCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTaa                                     <  2:2128786/128‑1 (MQ=255)
                                                                                     tttttATTTTTTTATAATAACAAAAAAAAAAAAAAAAATCCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTaaa                                    <  2:1175115/128‑1 (MQ=255)
                                                                                      ttttttttttttATTTCAAAAAAAAAAAAAAAAAAAATACCC‑‑‑TAAAAAATTAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTCTTATACCACATTTTATTTTTAAAAt                                  <  1:2248208/128‑1 (MQ=255)
                                                                                       ttttttttttTATATTAAAAAAAAAAAAAAAAAAAATAACC‑‑TTAAAAAAT‑‑AAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATa                                 <  1:3710645/128‑1 (MQ=255)
                                                                                        atATTTTTTTATAATAAAAAAAAAAAAAAAAACAATACCATT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAACTAAAAACAAAATCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATaa                                <  2:4528116/127‑1 (MQ=255)
                                                                                         ttttttttTATATTTACAAAAAAAAAAAAAACAATCCCATT‑‑‑AAAAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATaa                                <  2:3894114/126‑1 (MQ=255)
                                                                                         ttattGTTTATATTAAAAAAAAAAAAAAAAACAATCACATT‑‑‑AAAAAT‑‑TAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGa                              <  2:461539/125‑1 (MQ=255)
                                                                                             tttttATATTTAAAAAAAAAAAAAAAAAAAAACCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGata                            <  1:3749917/126‑1 (MQ=255)
                                                                                              ttttATTTTAACAAATAAAAAAAAAAAAATACCATT‑‑‑AAAAATTTAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATAtt                          <  1:3528588/127‑1 (MQ=255)
                                                                                                ttATATTAAAAAAAAAAAAAAAAACAATCCCATT‑‑‑AAAAAT‑‑TAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGAc                       <  2:1526830/128‑1 (MQ=255)
                                                                                                   tattataaaaaaTAAAAAAAAAACAAAACCCTT‑‑‑AAAAAT‑‑TAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACa                      <  1:4259520/119‑1 (MQ=255)
                                                                                                    tttttaaaaaaaaaaaaaaaaaaaATCCCC‑‑TTAAAAAAT‑‑‑AAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACat                     <  2:4436798/120‑1 (MQ=255)
                                                                                                     taataaaaaaTAAAAAAAAAACAATAACAAT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACa                      <  2:2385902/121‑1 (MQ=255)
                                                                                                        ttaaaaaaaaaaaaaaaaaaaaaaCCCTTAAAAATTAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACa                      <  2:2804957/124‑1 (MQ=255)
                                                                                                         taaaaaTAAAAAAAAAACAACTCCTTT‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAATAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACataat                  <  2:1268174/125‑1 (MQ=255)
                                                                                                              aTAAAAAAAAAACAATCCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAAAAAAAACACTATTTATTAAAAATAAAAATTATAACAAATTTTATTTTAAAAAAAAAAAATTAAAATAttatata             >  2:3402786/1‑128 (MQ=255)
                                                                                                                                   |                                                                                                                         
AAGTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCCTTTTAAAAAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAATATATATCTAATATATA  >  CP016816/202358‑202606

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: