BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP016816	311,148	3	.	T	57.2%	469.6 / inf	334	intergenic (‑71/‑243)	JCVSYN2_01300/JCVSYN2_01305	hypothetical protein/30S ribosomal protein S21
Reads supporting (aligned to +/- strand): ref base . (94/49); new base T (89/102); total (183/151)
Fisher's exact test for biased strand distribution p-value = 5.71e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.84e-03

Only 100 of 646 total aligned reads displayed.

TGTACCAGTTGTAATAACTAATCCAAATGAACTTAAAATTGTAATTAACTTTTTCATTTTACACACCCCTTATTAAAAAATAAAATACTTGTTACATACGATTCAAAACATATATAACATACAT‑TC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑A‑‑‑A‑T‑‑‑GCA‑‑‑ATATGTAAATGAAAATATGGTATAATAGCAACCGTTTATAAAGTAAAAAAATAGTAAATATACTACTTATTTACTAATAAAATCTATTTTTATTT  >  CP016816/311021‑311274

 gTACCAGTTGTAATAACTAATCCAAATGAACTTAAAATTGTAATTAACTTTTTCATTTTACACACCCCTTATTAAAAAATAAAATACTTGTTACATACGATTCAAAACATATATAACATAcat‑tc‑‑‑‑‑‑‑at‑‑‑‑‑‑‑‑t                                                                                                                                               >  1:1115284/1‑128 (MQ=255)

 gTACCAGTTGTAATAACTAATCCAAATGAACTTAAAATTGTAATTAACTTTTTCATTTTACACACCCCTTATTAAAAAATAAAATACTTGTTACATACGATTCAAAACATATATAACATAcat‑tc‑‑‑‑‑‑‑at‑‑‑‑‑‑‑‑t                                                                                                                                               >  1:4141975/1‑128 (MQ=255)

 gTACCAGTTGTAATAACTAATCCAAATGAACTTAAAATTGTAATTAACTTTTTCATTTTACACACCCCTTATTAAAAAATAAAATACTTGTTACATACGATTCAAAACATATATAACATAcat‑tc‑‑‑‑‑‑‑at‑‑‑‑‑‑‑‑t                                                                                                                                               >  1:1632822/1‑128 (MQ=255)

 gTACCAGTTGTAATAACTAATCCAAATGAACTTAAAATTGTAATTAACTTTTTCATTTTACACACCCCTTATTAAAAAATAAAATACTTGATACATACGATTCAAAACATATATAACATAcat‑tc‑‑‑‑‑‑‑tt‑‑‑‑‑‑‑‑t                                                                                                                                               >  2:3634405/1‑128 (MQ=255)

   aCCAGTTGTAATAACTAATCCAAATGAACTTAAAATTGTAATTAACTTTTTCATTTTACACACCCCTTATTAAAAAATAAAATACTTGTTACATACGATTCAAAACATATATAACATACAT‑TC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑t‑‑‑‑at                                                                                                                                         >  1:3924593/1‑128 (MQ=255)

   aCCAGTTGTAATAACTAATCCAAATGAACTTAAAATTGTAATTAACTTTTTCATTTTACACACCCCTTATTAAAAAATAAAATACTTGTTACATACGATTCAAAACATATATAACATACAT‑TC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑t‑‑‑‑at                                                                                                                                         >  1:1981988/1‑128 (MQ=255)

   aCCAGTTGTAATAACTAATCCAAATGAACTTAAAATTGTAATTAACTTTTTCATTTTACACACCCCTTAGTAAAAAATAAAATACTTGTTACATACGATTCAAAACATATATAACATACAT‑TC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑t‑‑‑‑at                                                                                                                                         >  1:2471217/1‑128 (MQ=255)

       gTTGTAATAACTAATCCAAATGAACTTAAAATTGTAATTAACTTTTTCATTTTACACACCCCTTATTAAAAAATAAAATACTTGTTACATACGATTCAAAACATATATAACATACAT‑TC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑t‑‑‑‑atatat                                                                                                                                     >  1:4319583/1‑128 (MQ=255)

       gTTGAAATAACTAATCCAAATGAACTTAAAAATGTAATTAACTTTTTCATTTTACACACCCCTTATTAAAAAATAAAATACTTGTTACATACGATTCAAAACATATATAACATACAT‑TC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑t‑‑‑‑atatat                                                                                                                                     <  1:692238/128‑1 (MQ=255)

        ttGTAATAACTAATCCAAATGAACTTAAAATTGTAATTAACTTTTTCATTTTACACACCCCTTATTAAAAAATAAAATACTTGTTACATACGATTCAAAACATATATAACATACAT‑TC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑t‑‑‑‑atatata                                                                                                                                    >  2:3680105/1‑128 (MQ=255)

          gTAATAACTAATCCAAATGAACTTAAAATTGTAATTAACTTTTTCATTTTACACACCCCTTATTAAAAAATAAAATACTTGTTACATACGATTCATAACATATATAACATACAT‑TC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑t‑‑‑‑atatatata                                                                                                                                  >  2:651749/1‑128 (MQ=255)

          gTAATAACTAATCCAAATGAACTTAAAATTGTAATTAACTTTTTCATTTTACACACCCCTTATTAAAAAATAAAATACTTGTTACATACGATTCAAAACATATATAACATACAT‑TC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑t‑‑‑‑atatatata                                                                                                                                  >  2:147218/1‑128 (MQ=255)

          gTAATAACTAATCCAAATGAACTTAAAATTGTAATTAACTTTTTCATTTTACACACCCCTTATTAAAAAATAAAATACTTGTTACATACGATTCAAAACATATATAACATACAT‑TC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑t‑‑‑‑atatatata                                                                                                                                  >  2:2434077/1‑128 (MQ=255)

          gTAATAACTAATCCAAATGAACTTAAAATTGTAATTAACTTTTTCATTTTACACACCCCTTATTAAAAAATAAAATACTTGTTACATACGATTCAAAACATATATAACATACAT‑TC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑t‑‑‑‑atatatata                                                                                                                                  >  1:3558634/1‑128 (MQ=255)

           taataaCTAATCCAAATGAACTTAAAATTCAAATTAACTTTTTCATTTTACACACCCCTTATTAAAATATAAAATACTTGTTACATACGATTCAAAACATATATAACAAACAA‑TC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑t‑‑‑‑atatatagat                                                                                                                                 >  2:4015427/1‑128 (MQ=255)

            aataaCTAATCCAAATGAACTTAAAATTGTAATTAACTTTTTCATTTTACACACCCCTTATTAAAAAATAAAATACTTGTTACATACGATTCAAAACATATATAACATACAT‑TC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑t‑‑‑‑atatatatata                                                                                                                                >  1:3490020/1‑128 (MQ=255)

             ataaCAAATCTAAATGAAATTAAAAGTTTAATTAACTTTTTAATTTTACACACCCCTTATTAAAAAATAAAATAATTGTTACATACGATTCAAAACATATATAACATACAT‑TC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑t‑‑‑‑atatatatatat                                                                                                                               <  2:2916749/128‑1 (MQ=255)

               aaCTAATCCAAATGAACTTAAAATTGTAATTAACTTTTTCATTTTACACACCCCTTATTAAAAAATAAAATACTTGTTACATACGATTCAAAACATATATAACATACAT‑TC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑t‑‑‑‑atatatatatatat                                                                                                                             >  2:3179870/1‑128 (MQ=255)

               aaCTAATCCAAATGAACTTAAAATTGTAATTAACTTTTTCATTTTACACACCCCTTATTAAAAAATAAAATACTTGTTACATACGATTCAAAACATATATAACATACAT‑TC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑t‑‑‑‑atatatatatatat                                                                                                                             >  2:4180671/1‑128 (MQ=255)

                 atAAACCAAATGAACATAAAATTTTAATTAACATTTTCATATTACACACACCTTATTAAAAAATAAAATACTTGTTACATACGATTCAAAACATATATAACATACAT‑TC‑‑‑‑‑‑‑AA‑‑‑‑‑‑‑‑t‑‑‑‑atatatatatatatat                                                                                                                           <  2:1226878/127‑1 (MQ=255)

                  tAATCCAAATGAACTTAAAATTGTAATTAACTTTTTCATTTTACACACCCCTTATTAAAAAATAAAATACTTGTTACATACGATTCAAAACATATATAACATACAT‑TC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑t‑‑‑‑atatatatatatatata                                                                                                                          >  2:3823899/1‑128 (MQ=255)

                    aTCCAAATGAACTTAAAATTGTAATTAACTTTTTCATTTTACACACCCCTTATTAAAAAATAAAATACTTGTTACATACGATTCAAAACATATATAACATACAT‑TC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑t‑‑‑‑atatatatatatatatata                                                                                                                        >  1:3093462/1‑128 (MQ=255)

                    aTCCAAATGAACTTAAAATTGTAATTAACTTTTTCATTTTACACACCCCTTATTAAAAAATAAAATACTTGTTACATACGATTCAAAACATATATAACATACAT‑TC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑t‑‑‑‑atatatatatatatatata                                                                                                                        >  2:1430335/1‑128 (MQ=255)

                     tacataagAAATTAAAATAGTAATTAACAATATAATTTTACACACACATAATTAAAAAATAAAATACTTGATACATACGAATCAAAACATATATAACATACAT‑TC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑t‑‑‑‑atatatatatatatatatat                                                                                                                       <  2:3095080/121‑1 (MQ=255)

                     taaatattaaattAAAATTGTAATTAAATTTTTTATTTTAAACACATATTATTAAAAAATAAAATAATTGTTAAATAATATTAAAAAAATATATAAAATACAT‑TA‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑t‑‑‑‑atatatatatatatatatat                                                                                                                       <  1:751103/117‑1 (MQ=255)

                     tCCAAATGAACTTAAAATTGTAATTAACTTTTTCATTTTACACACCCCTTATTAAAAAATAAAATACTTGTTACATACGATTCAAAACATATATAACATACAT‑TC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑t‑‑‑‑atatatatatatatatatat                                                                                                                       >  2:2264319/1‑128 (MQ=255)

                      ccAAATGAACTTAAAATTGTAATTAACTTTTTCATTTTACACACCCCTTATTAAAAAATAAAATACTTGTTACATACGATTCAAAACATATATAACATACAT‑TC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑t‑‑‑‑atatatatatatatatatata                                                                                                                      >  2:1746000/1‑128 (MQ=255)

                      ccAAATGAACTTAAAATTGTAATTAACTTTTTCATTTTACACACCCCTTATTAAAAAATAAAATACTTGTTACATACGATTCAAAACATATATAACATACAT‑TC‑‑‑‑‑‑‑AA‑‑‑‑‑‑‑‑t‑‑‑‑atatatatatatatatatata                                                                                                                      >  2:2320310/1‑128 (MQ=255)

                       cAAATTAACTAAAAATATTAATTAACTATTTCATTTTAAACACACCATATAAAAAAATAAAAAACTAGTTACATACTATTAAAAACATATATAACAAACAT‑AC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑t‑‑‑‑atatatatatatatatatatat                                                                                                                     <  2:4522322/128‑1 (MQ=255)

                       cAAATGAACTTAAAATTGTAATTAACTTTTTCATTTTACACACCCCTTATTAAAAAATAAAATACTTGTTACATACGATTCAAAACATATATAACATACAT‑TC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑t‑‑‑‑atatatatatatatatatatat                                                                                                                     >  1:3844418/1‑128 (MQ=255)

                         aaTGAACTTAAAATTGTAATTAACTTTTTCATTTTACACACCCCTTATTAAAAAATAAAATACTTGTTACATACGATTCAAAACATATATAACATACAT‑TC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑t‑‑‑‑atatatatatatatatatatatat                                                                                                                   >  2:608371/1‑128 (MQ=255)

                          aTGAACTTAAAAATGTAATTAACTTTTTAATTTTACACACCCCATAATAAAAAATAAAATACTTGTTACATACGATTCAAAACATATAAAACATACAT‑TC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑t‑‑‑‑atatatatatatatatatatatat‑‑a                                                                                                                <  2:787777/128‑1 (MQ=255)

                           ttatattAAAATTTTAATTTAATTTTTATTTTTAAACACAAATTATTAAAAAATAAAATATTTGTTATATACTATTTAAAAAATATATAACATATAT‑TC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑t‑‑‑‑atatatatatatatatatatatat‑‑at                                                                                                               <  1:2756524/123‑2 (MQ=255)

                           tGAACTTAAAATTGTAATTAACTTTTTCATTTTACACACCCCTTATTAAAAAATAAAATACTTGTTACATACGATTCAAAACATATATAACATACAT‑TC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑t‑‑‑‑atatatatatatatatatatatat‑‑at                                                                                                               >  1:2718335/1‑127 (MQ=255)

                           tGAACTTAAAATTGTAATTAACTTTTTCATTTTACACACCCCTTATTAAAAAATAAAATACTTGTTACATACGATTCAAAAAATATATAACATACAT‑TC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑t‑‑‑‑atatatatatatatatatatatat‑‑at                                                                                                               >  1:3416182/1‑127 (MQ=255)

                           tGAACTTAAAATTGTAATTAACTTTTTCATTTTACACACCCCTTATTAAAAAATAAAAGACTTGTTACATACGATTCAAAACATATATCACATACAT‑TC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑t‑‑‑‑atatatatatatatatatatatat‑‑ag                                                                                                               >  1:4448558/1‑127 (MQ=255)

                            gAACTTAAAATTGTAATTAACTTTTTCATTTTACACACCCCTTATTAAAAAATAAAATACTTGTTACATACGATTCAAAACATATATAACATACAA‑TC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑t‑‑‑‑atatatatatatatatatatatat‑‑ata                                                                                                              >  2:3482884/1‑126 (MQ=255)

                              aatttataTTTTAATTAAATTTTTTTTTTTAAACAAAAATTATTAAATAATAAAATTGTTGTTACATAATATTAAAAAAATATATAAAATATAT‑TT‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑a‑‑‑‑atatatatatatatatatatatat‑‑atata                                                                                                            <  1:4530752/121‑5 (MQ=255)

                              aCTTAAAATTGTAATTAACTTTTTCATTTTACACACCCCTTATTAAAAAATAAAATACTTGTTACATACGATTCAAAACATATATAACATACAT‑TC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑t‑‑‑‑atatatatatatatatatatatat‑‑ataaa                                                                                                            >  2:1043341/1‑124 (MQ=255)

                              aCTTAAAATTGTAATTAACTTTTTCATTTTACACACCCCTTATTAAAAAATAAAATACTTGTTACATAAGATTCAAAACATATATAAAATACAT‑TC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑t‑‑‑‑atatatatatatatatatatatat‑‑atata                                                                                                            <  1:3705547/128‑5 (MQ=255)

                               cTTAAAATTTTATTTATATTTTTTTTTTTAAACACCCCTTATTATAAAATAAAATACTTGTTAAATACGATTCAAAAAATATATAACATACAT‑TA‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑t‑‑‑‑atatatatatatatatatatatat‑‑atatat                                                                                                           <  1:3966870/128‑6 (MQ=37)

                               cTTAAAATTGTAATTAACTTTTTCATTTTACACACCCCTTATTAAAAAATAAAATACTTGTTACATACGATTCAAAACATATATAACATACAT‑TC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑t‑‑‑‑atatatatatatatatatatatat‑‑atatat                                                                                                           >  2:1187939/1‑123 (MQ=255)

                                ttAAAATTGTAATTAACTTTTTCATTTTACACACCCCTTATTAAAAAATAAAATACTTGTTACATACGATTCAAAACATATATAACATACAT‑TC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑t‑‑‑‑atatatatatatatatatatatat‑‑atataat                                                                                                          >  2:4320819/1‑122 (MQ=255)

                                 tAAAATTTTAATTAAATATTTTATTTTACACACATCTTATTATAAAATAAAATAATTTTTACATAAGATTCAAAACATATATAACATAAAT‑TC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑t‑‑‑‑atatatatatatatatatatatat‑‑atataatg                                                                                                         <  1:1360092/128‑8 (MQ=255)

                                   aaaTTGTAATTATATATTTCATTTTAAAAACCCCTTATTAAAAAAAAAAATAAATTTTATATAATATTCAAAAAATATATATAATAAAT‑TA‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑t‑‑‑‑atatatatatatatatatatatat‑‑atataatgaa                                                                                                       <  1:4365342/128‑10 (MQ=16)

                                   aaaTTGTAATTAACTTTTTCATTTTACACACCCCTTATTAAAAAATAAAATACTTGTTACATACGATTCAAAACATATATAACATACAT‑TC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑t‑‑‑‑atatatatatatatatatatatat‑‑atatataatg                                                                                                       >  2:3268543/1‑119 (MQ=255)

                                     aTTGTAATTAACTTTTTCATTTAACTCACCCCTTATTAAAAAATAAAATACTTGTTACATACGATTCAAAACATATATAACATACAA‑TC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑‑‑‑‑‑‑TATATATATATATATATATATAT‑‑A‑‑‑A‑T‑‑‑GCA‑‑‑ATATGTaa                                                                                         <  2:1102357/128‑1 (MQ=255)

                                      ttGTTATTAACTTTTTATTTTTAAAAACAATTTATTAAAAAATAAAATAGTTGTTAGATAAGATTTAAAAAATATATATCATAAAT‑TC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑t‑‑‑‑atatatatatatatatatatatat‑‑ataatgaatgtat                                                                                                    <  1:375605/128‑13 (MQ=16)

                                      ttGTAATTAACTTTTTCATTTTACACACCCCTTATTAAAAAATAAAATACTTGTTACATACGATTCAAAACATAAATAACATACAT‑TC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑T‑‑‑‑ATATATATATATATATATataaat‑‑a‑‑‑aataaataaata                                                                                                 >  2:2691570/1‑117 (MQ=255)

                                                cTTTTTCATTTTACACACCCCTTATTAAAAAATAAAATACTTGTTACATACGATTCAAAACATATATAACATACAT‑TC‑‑‑‑‑‑‑ATTA‑‑‑‑‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑A‑‑‑A‑T‑‑‑AAA‑‑‑ATATTTAAATTaaaa                                                                                  >  1:1207539/1‑128 (MQ=255)

                                                   tttAATTTTACACACAACTTATTAAAAAATAAAATACTTGTTACATAAGATTCAAAACATATATAACATACAT‑TC‑‑‑‑‑‑‑ATTATA‑‑‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑A‑‑‑A‑T‑‑‑GCA‑‑‑ATATGTAAATGAAAat                                                                                 <  2:1673017/128‑1 (MQ=255)

                                                         tttACACACCCCTTATTAAAAAATAAAATACTTGTTACATACGATTCAAAACATATATAACATACAT‑TC‑‑‑‑‑‑‑ATTATA‑‑‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑A‑‑‑A‑T‑‑‑TCA‑‑‑ATATCTAAATTAAAatatatta                                                                           >  2:1933218/1‑124 (MQ=255)

                                                           taaacaaaacTTATTAAAAAATAAAATACTTTTTATATATTATTCAAAAAATATATATAATATAT‑TA‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑A‑‑‑A‑T‑‑‑GCA‑‑‑ATATGTAAATGAAAATATGGTATAATAg                                                                     <  2:3920499/119‑1 (MQ=37)

                                                             cacacaACTTATTAAAAAATATAATAATTTTTACATACTATTCAAAAAATATATAACATACAT‑TC‑‑‑‑‑‑‑ATTATA‑‑‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑A‑‑‑A‑T‑‑‑GCA‑‑‑ATATGTAAATGAAAATATGGTataat                                                                       <  2:2795934/128‑1 (MQ=255)

                                                              acacacataattaAAAAATAAAATAATTGTTACATAATATTCAAAAAATATATAACATACAT‑TCAATATATATA‑‑‑‑‑‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑A‑‑‑A‑T‑‑‑GCA‑‑‑ATATGTAAATGAAAATATGGtat                                                                          <  2:376429/119‑1 (MQ=255)

                                                              acacaaattattaAAAAATAAAATAATTGTTACATAATATTAAAAAAATATATAAAATAAAT‑TC‑‑‑‑‑‑‑ATTATATA‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑A‑‑‑A‑T‑‑‑GCA‑‑‑ATATGTAAATGAAAATATGGTataa                                                                        <  1:1698721/121‑1 (MQ=255)

                                                                actacTTATTAAAAAATAAAATATTTTTTAGATATTATTAAAAAAATATATAACATAAAT‑TA‑‑‑‑‑‑‑ATTATATATAT‑‑‑‑ATATATATATATATATATATATAT‑‑A‑‑‑A‑T‑‑‑GCA‑‑‑ATATGTAAATGAAAATATGGTataa                                                                        <  1:1458218/124‑1 (MQ=16)

                                                                   ttatataaAAAAATAAAATTTTTTTTATATTATTTTAAAAATATATAAAATATATATATA‑‑‑‑‑‑‑ATA‑‑‑‑‑‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑A‑‑‑A‑T‑‑‑GCA‑‑‑ATATGTAAATGAAAATATGGTATAATAGCAACCg                                                               <  2:3902187/125‑1 (MQ=255)

                                                                     ttattaAAAAATAAAATACTTGTTACATACGATTCAAAACATATATAACATACAT‑TC‑‑‑‑‑‑‑ATTATA‑‑‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑A‑‑‑A‑T‑‑‑TCA‑‑‑ATATTTATATGAAAATATGTTATTATATCAAcag                                                               >  1:636038/1‑126 (MQ=255)

                                                                      tattaAAAAATAAAATACTTGTTACATACGATTCAAAACATATATAACATACAT‑TC‑‑‑‑‑‑‑ATTATATA‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑A‑‑‑A‑T‑‑‑GCT‑‑‑ATATGTAAATTAAAATATGTTATTATAGCaaaa                                                                >  2:502009/1‑126 (MQ=255)

                                                                      tattaAAAAATAAAATACTTGTTACATACGATTCAAAACATATATAACATACAT‑TC‑‑‑‑‑‑‑ATTATA‑‑‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑A‑‑‑A‑T‑‑‑TCA‑‑‑ATATTAAAATAAAAATATTATATAATATCAAcata                                                              >  2:1856357/1‑125 (MQ=255)

                                                                        ttaAAAAATAAAATACTTGTTACATACGATTCAAAACATATATAACATACAT‑TC‑‑‑‑‑‑‑ATTATATA‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑A‑‑‑A‑T‑‑‑GCA‑‑‑ATATGTAAATAAAAATATTGTATAATATCAAcaat                                                              >  2:4423992/1‑125 (MQ=255)

                                                                          aaaaaaTAAAATACTTGTTACATACGATTCAAAACATATATAACATACAT‑TC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑AT‑‑A‑TAAAAAA‑‑‑ATATGTAAATGAAAATATTTTATTATAGAAAACGTTtat                                                          >  1:3344487/1‑128 (MQ=255)

                                                                            aaaatataatAATTTTTAAATAAGATTCAAAAAATATATAACAAAAAT‑TC‑‑‑‑‑‑‑ATTATATA‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑A‑‑‑A‑T‑‑‑GCA‑‑‑ATATGTAAATGAAAATATGGTATAATAGCAACCGTTtat                                                          <  2:3361883/128‑1 (MQ=255)

                                                                               ataaaataCTTGTTACATACGATTCAAAACATATATAACATACAT‑TC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑t‑‑‑‑atatatatatatatatatatatat‑‑a                                                                                                                >  1:798099/1‑75 (MQ=255)

                                                                                 ataataatTGTTTTATACTATTCAAAACATATATAACATAAAT‑TA‑‑‑‑‑‑‑TTTTTA‑‑‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑A‑‑‑A‑T‑‑‑GCA‑‑‑ATATGTAAATGAAAATATGGTATAATAGCAACCGTTTATAAAGTaa                                                   <  1:2132187/126‑1 (MQ=255)

                                                                                 aaaataCTTGTTACATACGATTCAAAACATATATAACATACAT‑TC‑‑‑‑‑‑‑ATTA‑‑‑‑‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑A‑‑‑A‑T‑‑‑GCA‑‑‑ATATGTAAATTAAAATATTTTATAATAGCAACCGTTTATAAAGTaaaa                                                 >  1:567218/1‑128 (MQ=255)

                                                                                 aaaataATTTTTAAATAATATTCAAAAAATATATAACATACAT‑TC‑‑‑‑‑‑‑ATTATATA‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑A‑‑‑A‑T‑‑‑GCA‑‑‑ATATGTAAATGAAAATATGGTATAATAGCAACCGTTTATAAAGt                                                     <  2:3972928/128‑1 (MQ=255)

                                                                                  aaataCTTGTTACATACGATTCAAAACATATATAACATACAT‑TC‑‑‑‑‑‑‑ATTATATA‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑A‑‑‑A‑T‑‑‑TAA‑‑‑ATATGTAAAATAAAATATTTTATAATAGAAACATTTTATAAATTa                                                    >  1:46327/1‑128 (MQ=19)

                                                                                  aaataATTGTTACATACGATTAAAAACATATATAACATACAT‑TC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑A‑‑‑A‑T‑‑‑GCA‑‑‑ATATGTAAATGAAAATATGGTATAATAGCAACCGTTTATAAAGTaaaaaaa                                              <  1:179007/128‑1 (MQ=255)

                                                                                      aCTTGTTACATACGATTCAAAACATATATAACATACAT‑TC‑‑‑‑‑‑‑ATTATA‑‑‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑A‑‑‑A‑T‑‑‑GAA‑‑‑ATCTTAAAATAAAAATATTATAATATAGAAAGATTATATAAAGTaaaaaaa                                              >  2:1975382/1‑128 (MQ=16)

                                                                                       cTTGTTACATACGATTCAAAACATATATAACATACAT‑TC‑‑‑‑‑‑‑ATTATATA‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑A‑‑‑A‑T‑‑‑TCT‑‑‑ATATGTAAATGAAAATATGGTATAATAGCAAACGTTTATAAAGTaaaaat                                               >  1:519595/1‑127 (MQ=255)

                                                                                       cTTGTTACATACGATTCAAAACATATATAACATACAT‑TC‑‑‑‑‑‑‑ATTA‑‑‑‑‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑A‑‑‑A‑T‑‑‑GCA‑‑‑ATATGTAAATGAAAATATTGTATTATAGCAAACTTTTATATAGTAAAAAAATAg                                           >  1:1588778/1‑128 (MQ=255)

                                                                                       cTTGTTACATACGATTCAAAACATATATAACATACAT‑TC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑ATATA‑TAAAAAA‑‑‑ATATTTAAATGAAAATATTTTATAATAGAAACAATTTATAAATTaaaaaa                                               >  1:2309411/1‑128 (MQ=255)

                                                                                        ttGTTACATACGATTCAAAACATATATAACATACAT‑TC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑ATATA‑TAAATCA‑‑‑ATATGTAAATGAAAATATGATATAATAGAAAAATTTTATAAATaaaaaaaa                                              >  1:1923720/1‑128 (MQ=255)

                                                                                         tGTTACATACGATTCAAAACATATATAACATACAT‑TC‑‑‑‑‑‑‑ATTATATA‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑A‑‑‑A‑T‑‑‑GAA‑‑‑ATATTTAAATGAAAATATGTTATAATAGCAAAAGTTTATAAATTAAAAAAAt                                             >  1:2186214/1‑128 (MQ=25)

                                                                                         tGTTACATACGATTCAAAACATATATAACATACAT‑TC‑‑‑‑‑‑‑ATTATA‑‑‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑A‑‑‑A‑T‑‑‑GAA‑‑‑ATATGTATATTAAAATATGTTTTATTATAAACAGTTTATATAATAAAAAtatat                                           >  1:898780/1‑127 (MQ=19)

                                                                                          gTTACATAATATTCTAAAAATATATAAAATACAT‑TCTTT‑‑‑‑ATA‑‑‑‑‑‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑A‑‑‑A‑T‑‑‑GCA‑‑‑ATATGTAAATGAAAATATGGTATAATAGCAACCGTTTATAAAGTAAAAAAATAGt                                          <  2:1622711/128‑1 (MQ=255)

                                                                                            tACATACGATTCAAAACATATATAACATACAT‑TC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑ATATA‑T‑‑‑AAATATATATTTAAAAGAAAATATTGTATAATAGAAACAGTTTATAATTTAAAAAAATaat                                          >  1:2556159/1‑126 (MQ=255)

                                                                                             agaTACTATTAAAAAAATATATAACATACAT‑TA‑‑‑‑‑‑‑ATTATATA‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑A‑‑‑A‑T‑‑‑GCA‑‑‑ATATGTAAATGAAAATATGGTATAATAGCAACCGTTTATAAAGTAAAAAAATAGTa                                         <  1:3629639/126‑1 (MQ=25)

                                                                                              cATACGATTCAAAACATATATAGCATACAT‑TC‑‑‑‑‑‑‑ATTATATA‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑A‑‑‑A‑T‑‑‑TAA‑‑‑ATATATAAAATAAAATATTTTATAATATCAAACTTTTATAAATAAAAAAAATAGTaa                                        >  1:100063/1‑128 (MQ=16)

                                                                                                taagATTAAAAACATATATAACATAAAT‑TC‑‑‑‑‑‑‑ATTATATA‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑A‑‑‑A‑T‑‑‑GCA‑‑‑ATTTGTAAATGAAAATATGGTATAATAGCAACCGTTTATAAAGTAAAAAAATAGTAAat                                      <  2:2615936/125‑1 (MQ=255)

                                                                                                tACGATTCAAAACATATATAACATACAT‑TC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑T‑‑‑‑ATATATATATATATATATATATATAAA‑‑‑A‑T‑‑‑TAA‑‑‑ATATATAAAAAAAAATATATTATAATATAAAAAGTTTATAAAATAAAAAAATATTAAAaataa                                  >  2:708433/1‑127 (MQ=255)

                                                                                                 atcaTTAAAAACATATATAACATACAT‑TC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑TATAAATATATATATATATATATATATAT‑‑A‑‑‑A‑T‑‑‑GCA‑‑‑ATATGTAAATGAAAATATGGTATAATAGCAACCGTTTATAAAGTAAAAAAATAGTAAAtata                                   <  1:1283109/125‑1 (MQ=255)

                                                                                                 aCGATTCAAAACATATATAACATACAT‑TC‑‑‑‑‑‑‑ATTATA‑‑‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑A‑‑‑A‑T‑‑‑GCA‑‑‑ATATGTAAATGAAAATATGGTATAATAGCAACCGTTTATAAAGTAAAAAAATAGTAAAtata                                   <  2:3538599/128‑1 (MQ=255)

                                                                                                  cGATTCAAAACATATATAACATACAT‑TC‑‑‑‑‑‑‑ATTA‑‑‑‑‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑A‑‑‑A‑T‑‑‑GAA‑‑‑ATATTTAAATTAAAATATGTTATAATAGCAACAGTATATAAATTAAAAAAATATTAAATAtacaa                                >  2:4085834/1‑126 (MQ=37)

                                                                                                  cGATTCAAAACATATATAACATACAT‑TC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑AT‑‑ACT‑‑‑GCA‑‑‑ATATGTAAATGAAAATATGTTATATTAGAAAACGTTTATAAATAAAAAAAATAGTATATataata                                >  1:2223403/1‑128 (MQ=255)

                                                                                                   gATTAAAAACATATATATCATACAT‑TA‑‑‑‑‑‑‑ATTATATA‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑A‑‑‑A‑T‑‑‑GCA‑‑‑ATATGTAAATGAAAATATGGTATAATAGCAACCGTTTATAAAGTAAAAAAATAGTAAAtata                                   <  1:282889/128‑1 (MQ=37)

                                                                                                      tCAAAACATATATAACATACAT‑TC‑‑‑‑‑‑‑ATTATATA‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑A‑‑‑A‑T‑‑‑AAA‑‑‑ATATTTAAATTAAAATATTTTATAATATCAAGATTTAATAAAATAAAAAAATAGtatataatcta                                >  2:3006825/1‑123 (MQ=255)

                                                                                                      tCAAAACATATATAACATACAT‑TC‑‑‑‑‑‑‑ATTATA‑‑‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑A‑‑‑A‑T‑‑‑TAA‑‑‑ATATATATATTAAAATATTATATAATATAAAAAGTTTATAAATAAAAAAAATATAAAATataataat                              >  2:862168/1‑126 (MQ=12)

                                                                                                        aaaaCATATATAACATACAT‑TC‑‑‑‑‑‑‑ATTA‑‑‑‑‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑A‑‑‑A‑T‑‑‑TCA‑‑‑ATATGTAAATTAAAATATTTTATAATATCAACAGTTTATAAATTAAAAAAATATTAAATATACTAattatt                          >  1:4235332/1‑128 (MQ=255)

                                                                                                         aaaCATATATAACATACAT‑TC‑‑‑‑‑‑‑ATTATA‑‑‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑A‑‑‑A‑T‑‑‑TCA‑‑‑ATATTTAAATGAAAATATTTTATAATATCAACCTTTTATAAATTAAAAAAATATTAAATATACTAATTAt                           >  1:4022519/1‑128 (MQ=255)

                                                                                                           aCATATATAACATACAT‑TC‑‑‑‑‑‑‑ATTATATA‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑A‑‑‑A‑T‑‑‑TAA‑‑‑ATATTTATATTAAAATATTTTATAATATAAAAAGATTATAAATTAAAAAAATATTAAATATAATAATTaa                           >  2:1552395/1‑127 (MQ=12)

                                                                                                            tatataaAACATATAT‑TA‑‑‑‑‑‑‑TA‑‑‑‑‑‑‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑A‑‑‑T‑T‑‑‑GCA‑‑‑ATATGTAAATGAAAATATGGTATAATAGCAACCGTTTATAAAGTAAAAAAATAGTAAATATACTACTTATTTACtaa                    <  2:1332996/127‑1 (MQ=255)

                                                                                                            cATATATAACATACAT‑TC‑‑‑‑‑‑‑ATTA‑‑‑‑‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑A‑‑‑A‑T‑‑‑GCA‑‑‑ATATGTAAATTAAAATATTGTATAATAGCAACCGTTTATAAAGTAAAAAAATAGTAAATATAATACTTATTTaat                      >  1:3229542/1‑126 (MQ=255)

                                                                                                              tatataACATACAT‑TC‑‑‑‑‑‑‑ATTA‑‑‑‑‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑A‑‑‑A‑A‑‑‑TAA‑‑‑ATATTTAAATTAAAATATGTTATAATATCAACCGTTTATAAAGTAAAAAAATATAAAATATAAAAATTATTtaataa                    >  1:3028109/1‑128 (MQ=255)

                                                                                                               atataACATACAT‑TC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑AT‑‑A‑TAAATCA‑‑‑ATATTTAAAATAAAATATTTTATAATAGAAACATTTTATAAATAAAAAAAATAATAAATATAATAattattaaata                     >  1:407852/1‑123 (MQ=255)

                                                                                                                tataACATACAT‑TC‑‑‑‑‑‑‑ATTATA‑‑‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑A‑‑‑A‑T‑‑‑GCA‑‑‑ATATGTAAATGAAAATATGGTATAATAGCAACCGTTTATAAAGTAAAAAAATAGTAAATATACTACTTATTTACtaa                    <  2:2228030/128‑1 (MQ=255)

                                                                                                                tataACATACAT‑TC‑‑‑‑‑‑‑ATTA‑‑‑‑‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑A‑‑‑A‑T‑‑‑GCA‑‑‑ATATGAAAATGAAAATATTTTATAATAGAAACATTTTATAAAATAAAAAAATAGTAAATAAACTACTTATTtaataaaa                  >  1:2672144/1‑126 (MQ=255)

                                                                                                                 ataACATACAT‑TC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑AT‑‑A‑TAAATCA‑‑‑ATATGTAAATTAAAATATGTTATAATAGAAACATTTTATAAAGTAAAAAAATAATAAATATACTAATTATTTACtaat                   >  2:2098071/1‑128 (MQ=255)

TGTACCAGTTGTAATAACTAATCCAAATGAACTTAAAATTGTAATTAACTTTTTCATTTTACACACCCCTTATTAAAAAATAAAATACTTGTTACATACGATTCAAAACATATATAACATACAT‑TC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑A‑‑‑A‑T‑‑‑GCA‑‑‑ATATGTAAATGAAAATATGGTATAATAGCAACCGTTTATAAAGTAAAAAAATAGTAAATATACTACTTATTTACTAATAAAATCTATTTTTATTT  >  CP016816/311021‑311274

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`