Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 19,523 (A)11→10 intergenic (‑244/‑893) JCVSYN2_00065 ← / → JCVSYN2_00070 hypothetical protein/tetracycline resistance ribosomal protection protein Tet(M)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP01681619,5130A.91.9% 5184.3 / inf 1590intergenic (‑234/‑903)JCVSYN2_00065/JCVSYN2_00070hypothetical protein/tetracycline resistance ribosomal protection protein Tet(M)
Reads supporting (aligned to +/- strand):  ref base A (79/43);  new base . (749/633);  total (857/733)
Fisher's exact test for biased strand distribution p-value = 2.87e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1764 total aligned reads displayed.

CTTCGTTTATCTTGCCTGCTCATTTTTTAGTATATTCTTCGAAGAAATCACATTACTTTATATAATGTATAATTCATTATGTGATAATGCCAATCGCTAAGAAAAAAAAAGAGTCATCCGCTAGGTGGAAAAAAAAAAATGAAAATCATTACCGAGGCATAAAAAAATATAGAGTGTACTAGGATCGCTTGCCTGTAACTTACACGCGCCTCGTATCTTTTAATGATGGAATAATTTGGGAATTTACTCTGTGTT  >  CP016816/19385‑19639
                                                                                                                                 |                                                                                                                              
 ttCGTTTATCTTGCCTGCTCATTTTTTAGTATATTCTTCGAAGAAATCACATTACTTTATATAATGTATAATTCATTATGTGATAATGCCAATCGCTAAGAAAAAAAAAGAGTCATCCGCTAGGTGGa                                                                                                                                >  2:526089/1‑128 (MQ=255)
 ttCGTTTATCTTGCCTGCTCATTTTTTAGTATATTCTTCGAAGAAATCACATTACTTTATATAATGTATAATTCATTATGTGATAATGCCAATCGCTAAGAAAAAAAAAGAGTCATCCGCTACGTGGa                                                                                                                                >  2:2881291/1‑128 (MQ=255)
  tCGTTTATCTTGCCTGCTCATTTTTTAGTATATTCTTCGAAGCAATCACATTACTTTATATAATGTATAATTCATTATGTGATAATGCCAATCGCTAATAAAAAAAAAGAGTCATCCGCTATGTGGaa                                                                                                                               >  1:2533860/1‑128 (MQ=255)
  tCGTTTATCTTGCCTGCTCATTTTTTAGTATATTCTTCGAAGAAATCACATTACTTTATATAATGTATAATTCATTATGTGATAATGCCAATCGCTAAGAAAAAAAAAGAGTCATCCGCTAGGTGGaa                                                                                                                               >  2:2531310/1‑128 (MQ=255)
   cGTTTATCTTGCCTGCTCATTTTTTAGTATATTCTTCGAAGAAATCACATTACTTTAAATAATGTATAATTCATTATGTGATAATGCCAATCGCTAAGAAAAAAAAAGAGTCATCCGCTAGGTGGaaa                                                                                                                              <  2:2180724/128‑1 (MQ=255)
   aagatctCTTTCCTGATCATTTTTTATTATATTCTTCGAAGAAATCAAATTACTTTATATAATGTATAATTCATTATTTGATAATGCCAATCGCTAAGAAAAAAAAAGAGTCATCCGCTAGGTGGaaa                                                                                                                              <  2:4284407/122‑1 (MQ=255)
    gTTTATCTTGCCTGCTCATTTTTTAGTATATTCTTCGAAGAAATCACATTACTTTATATAATGTATAATTCATTATGTGATAATGCCAATCGCTAAGAAAAAAAAAGAGTCATCCGCTAGGTGGaaaa                                                                                                                             >  1:2768454/1‑128 (MQ=255)
      ttATCTTGCCTGCTCATTTTTTAGTATATTCTTCGAAGAAATCACATTACTTTATATAATGTATAATTCATTATGTGATAATGCCAATCTCTAAGAAAAAAAAAGAGTCAACCGCTAGGTGGaaaaaa                                                                                                                           >  2:665824/1‑128 (MQ=255)
      ttATCTTGCCTGCTCATTTTTTAGTATATTCTTCGAAGAAATCACATTACTTTATATAATGTATAATTCATTATGTGATAATGCCAATCGCTAAGAAAAAAAAAGAGTCATCCGCTAGGTGGaaaaaa                                                                                                                           >  2:4427521/1‑128 (MQ=255)
       tataTTGCCTGCTCATTTTTTTGTATTTTCTTCTAAGAAATCAAATTACTTTATATAATGTATAATTCATTATGTGATAATGCCAATCGCTAAGAAAAAAAAAGAGTCATCCGCTAGGTGGaaaaaaa                                                                                                                          <  1:1895403/128‑1 (MQ=255)
       tATCTTTCCTGCTCATTTTTTAGTATATTCTTCGAAGAAATCACATTACTTTATATAATGTATAATTCATTATGTGATAATTCCAATCGCTAAGAAAAAAAAATAATCATCATCTAGATGaaaaaaaa                                                                                                                          >  1:10020/1‑128 (MQ=255)
               cTGCTCATTTTTTAGTATATTCTTCGAAAAAATCACATTAATTTATATAATGTATAATACATTATTTTATAATGACAATCGCTAATAAAAAAAAATAATCATCCGCTAGGTTGAAAAATAAAATGaaa                                                                                                                 >  1:1353621/1‑128 (MQ=255)
                    caatttttAGTATATTCTTCTAAGAAATCACATTACTTTATATAATGTATAATTAATTATGTGATAATGCCAATCGCTAATAAAAAAAAAGAGTCATCCGCTAGGTGGAAAAAAAAAATGAAAATCAt                                                                                                            <  1:4237428/125‑1 (MQ=255)
                        ttttAGTATATTCTTCGAAGAAATCACATTACTTTATATAATGTATAATTCATTATGTGATAATGCCAATCGCTAAGAAAAAAAAAGAGTCATCCGCTAGGTGGAAAAAAAAAATGAAAATCATTAcc                                                                                                        >  2:3407502/1‑128 (MQ=255)
                        tttaAGTATATTCTTCGAAGAAATCAAATTACTTTATATAATGTATAATTCATTTTGTGATAAAGCCAAACGATAAGAAAAAAAAATAGTCATCCGCTAGGTGGAAAAAAAAAATGAAAATCATTAcc                                                                                                        <  2:3643431/128‑1 (MQ=255)
                        ttaccttcaatTCTTCGAATAAATCAAAATACATAATAAAAATTATAATTCATAATGTGATAATTCCAATCCCAAACAAAAAAAAAGATTCATACCCTAGGTGGAAAAAAAAAATGAAAATCATTAcc                                                                                                        <  2:1388347/119‑1 (MQ=255)
                         tttAGTATATTCTTCGAAGAAATCACATTACTTTATATAATGTATAATTCATTATGTGATAATGCCAATCTCTAAGAAAAAAAAAGAGTCATCCGCTAGGTGGAAAAAAAAAATGAAAATCATTAcag                                                                                                       >  2:3208938/1‑126 (MQ=255)
                         tttAGTATATTCTTCGAAGAAATCACATTACTTTATATAATGTATAATTCATTATGTGATAATGCCAATCGCTAAGAAAAAAAAAGAGTCATCCGCTAGGTGGAAAAAAAAAAATGAAAATCATTAc                                                                                                         >  1:1080933/1‑127 (MQ=255)
                         tttAGTATATTCTTCGAAGAAATCACATTACTTTATATAATGTATAATTCATTATGTGATAATGCCAATCGCTAAGAAAAAAAAAGAGTCATCCGCTAGGTGGAAAAAAAAAATGAAAATCATTACCg                                                                                                       >  2:805249/1‑128 (MQ=255)
                         tttAGTATATTCTTCGAAGAAATCACATTAATTTATATAATGTATAATTCATTATGTGATAATGCCAATCGCTAAGAAAAAAAAAGAGTCATCCGCTAGGTGGAAAAAAAAAATGAAAATCATTACCg                                                                                                       <  2:730126/128‑1 (MQ=255)
                              tataTTCTTCGAAGAAATCACATTACTTTATATAATGTATAATTCATTATGTGATAATGCCAATCGCTAAGAAAAAAAAAGAGTCATCCGCTAGGTGGAAAAAATAAATGAAAATCATTACAGAGACa                                                                                                  >  1:259761/1‑128 (MQ=255)
                                 atTCTTCGAAGAAATCACATTACTTTATATAATGTATAATTCATTATGTGATAATGCCAATCGCTAAGAAAAAAAAAGAGTCATCCGCTAGGTGGAAAAAAAAAATGAAAATCATTACCGAGGCATaa                                                                                               >  1:2253898/1‑128 (MQ=255)
                                 atTCTTCGAAGAAATCACATTACTTTATATAATGTATAATTCATTATGTGATAATGCCAATCGCTAAGAAAAAAAAAGAGTCATCCCCTAGGTGGAAAAAAAAAATAAAAAAAATTCCCGAggaaaaa                                                                                               >  1:346545/1‑123 (MQ=255)
                                  ttattagaataaATAAAATTACTTTATATAATGTATATTTTATTATGTGTTAATGCCATTCTCTTAGTAAAATATTGAGTCTTCTGCTTTGTGTAAAAAAAAAATGAAAATCATTACCGAGGCATaaa                                                                                              <  1:1664030/122‑1 (MQ=255)
                                   tcttcGAAGAAATCACATTACTTTATATAATGTATAATTCATTATGTGATAATGCCAATCTCTAAGAAAAAAAAATAGTCATCCGCTAGGTGTAAAAAAAAAATGAAAAACATTACAGAGGAATaaaa                                                                                             >  1:1592645/1‑128 (MQ=255)
                                   tcttcGAAGAAATCACATTACTTTATATAATGTATAATTCATTATGTGATAATGCCAATCGCTAAGAAAAAAAAAGAGTCATCCGCTAGGTGGAAAAAAAAAATGAAAATCCATACCGAGGCATaaaa                                                                                             >  2:589333/1‑128 (MQ=255)
                                   tcttcGAAGAAATCACATTACTTTATATAATGTATAATTCATTATGTGATAATGCCAATCGCTAAGAAAAAAAAAGAGTCATCCGCTAGGTGGAAAAAAAAAATGAAAATCATTACCGAGGCATaaaa                                                                                             >  1:3639671/1‑128 (MQ=255)
                                   tcttcGAAGAAATCACATTACTTTATATAATGTATAATTCATTATGTGATAATGCCAATCGCTAAGAAAAAAAAAGAGTCATCCGCTACGTGGAAAAAAAAAATGAAAATCATTAGCGGGGCATaaaa                                                                                             >  1:825465/1‑128 (MQ=255)
                                    cttcGAAGATATAACATTATTTAATAAATTGTATAATTCATTATGTGATAATGCCAATCGCTAAGAAAAAAAAAGAGTCATCCGCTAGGTGGAAAAAAAAAATGAAAATCATTACCGAGGCATaaaaa                                                                                            <  1:2311863/128‑1 (MQ=255)
                                     ttcGAAGAAATCACATTACTTTATATAATGTATAATTCATTATGTGATAATGCCAATCGCTAAGAAAAAAAAAGAGTCATCCGCTAGGTGGAAAAAAAAAATGAAAATCATTACCGAGGCATaaaaaa                                                                                           >  2:3939455/1‑128 (MQ=255)
                                         aagaaATCACATTACTTTATATAATGTATAATACATTATGTGATAATGCCAATCGCTAAGAAAAAAAAAGAGTCCTCCGCAACGTGGAAAAAAAAAATGAAAATCATTACCGATGAGTAAAAAAatat                                                                                       >  2:2963586/1‑128 (MQ=255)
                                           gaaATCACATTACTTTATATAATGTATAATTCATTATGTGATAATGCCAATCGCTAAGAAAAAAAAAGAGTCATCCGCTACGTGGAAAACAACAATGAAAATCATTCCAGAGGCATAAATCACTATag                                                                                     >  2:4117443/1‑128 (MQ=255)
                                            aaaTCACATTACTTTATATAATGTATAATTCATTATGTGATAATGCCAATCGCTAAGAAAAAAAAAGAGTCATCCGCTAGGTGGAAAAAAAAAATGAAAATCATTACCGAGGCATAAAAAAATATaga                                                                                    >  1:2465420/1‑128 (MQ=255)
                                            aaaTCACATTACTTTATATAATGTATAATTCATTATGTGATAATGCCAATCGCTAAGAAAAAAAAAGAGTCATCCGCTAGGTGG‑‑AAAAAAAAATGTAAATCAATACCGAGTCATAAAAAAAaatataa                                                                                   >  1:3295070/1‑125 (MQ=255)
                                             aataaaattattTTATATTATTTATAATTCATTATGTGATAATTCCAATAGCTAAGAAAAAAAAAGAGTCATCCGCTAGGTGGAAAAAAAAAATGAAAATCATTACCGAGGCATAAAAAAATATagag                                                                                   <  1:4145239/122‑1 (MQ=255)
                                             aaTCACATTACTTTATATAATGTATAATTCATTATGTGATAATGCCAATCGCTAAGAAAAAAAAAGAGTCATCCGCTAGATGTAAAAAAAAAATTAAAATCATTACCTAAACATAAAAAAataaaaat                                                                                   >  1:595313/1‑123 (MQ=255)
                                              atattaTTTCTTTATATAATGTATAAATAATTATGTGAATATGAGAATCTCTAAGATAAAAAAAGACGCATCCGCTAGGTGGAAAAAAAAAATGAAAAGCATTACCGAGGCATAAAAAAATATAGCgt                                                                                  <  2:4019405/123‑1 (MQ=255)
                                              aTCAAATTAATTTTTATAATTTATAATTCATTATGTTATAATGCCAATCGCTAAGAAAAAAAAAGAGTCATCCGCTAGGTGGAAAAAAAAAATGAAAATCATTACCGAGGCATAAAAAAATATTGAgt                                                                                  <  1:406454/128‑1 (MQ=255)
                                                 acaTTACTTTATATAATGTTTAATTCATTATGTGATAATGCCCAACGGAAAGAAAAAACAAGAGGCATCCGCTAGGTGGAAAAAAAAAATGAAAATCATTACCGAGGCATAAAAAAATATAGAGTGTa                                                                               <  1:767246/128‑1 (MQ=255)
                                                 acaTTACTTTATATAATGTATAATTCATTATGTGATAATGCCAATCGCTAAGAAAAAAAAAGAGTCATCCGCTAGGTGGAAAAAAAAAATGAAAATCATTACCGAGGCATAAAAAAATATAGAGTGTa                                                                               <  2:1002279/128‑1 (MQ=255)
                                                 acaTTAATTTATATAATGTATAATTCATTATGTGAAAATTCAAATCGCTAAGAAAAAAAAAGATTAATCAGCTAGGTGGAAAAAAAAAATGAAAATCATTACCGAGGCATAAAAAAATATAGAGTGTa                                                                               <  2:3308211/128‑1 (MQ=255)
                                                 acaTAACTTTATATAATGTATAATTAATTATGTTAAAATGCCAATCGCAAAGAAAAAAAAAGATTCATACGCTACGTGGAAAAAAAAAATGAAAATCATTACCGAGGCATAAAAAAATATAGAGTGTa                                                                               <  2:4546126/128‑1 (MQ=255)
                                                  caTTCCTTTATATAAAGTATACTTCATTATGTGATAATGCCAATCGCTAAGAAAAAAAAGAGTCATCCGCTAGGTGGAAAAAAAAAATGAAAATCATTACCGAGGCATAAAAAAATATAGAGTGTACt                                                                             <  1:3003734/128‑1 (MQ=255)
                                                  caTTACTTTATATAATGTATAATTCATTATGTGATAATGCCAATCGCTAAGAAAAAAAAAGAGTCATCCGCTAGGTGGAAAAAAAAAATGAAAATCATTACCGAGGCATAAAAAAAAATAAATTGTAc                                                                              >  1:4507294/1‑128 (MQ=255)
                                                     tACTTTAAATAATGTATAATTGATTATGTGATAAAGCCAATCGCTAAGAAAAAAAAAGAGTCATCCGCTAGGTGGAAAAAAAAAATGAAAATCATTACCGAGGCATAAAAAAATATAGAGTGTACTAg                                                                           <  2:2618813/128‑1 (MQ=255)
                                                      aCTTTATATAATGTATAATTCATTATGAGATAATTCCAAACGATATCAAAAAAAAAGACTCATCCGCTAGGTGGAAAAAAAAAAAGAAAAACAATACAGAGCCAAAAAAAAAGATagaaagaactatg                                                                          >  2:1968981/1‑118 (MQ=255)
                                                       tataaagtaATGTATAAATCATTATGTGATAATGCCAATCGCTAAGAAAAAAAAAGAGTCATCCGCTAGGTGGAAAAAAAAAAATGAAAATCATTACCTAGGCATAAAAAAAAATAGAGTGTAATAgg                                                                          >  1:1781170/8‑128 (MQ=255)
                                                       cTTTATATAATGTATAATTCATTATGTGATAATGCCAATCGCTAAGAAAAAAAAAGAGTCATCCGCTAGGTGGAAAAAAAAAATGAAAATCATTACCGAGGCATAAAAAAATATAGAGTGTACTAGGa                                                                         <  2:2060491/128‑1 (MQ=255)
                                                        tatatataATGTATAATTCATTATGTGATAATGCCAATCGCTAAGAAAAAAAAAGAGTCATCCGCTAGGTGGAAAAAAAAAATGAAAATCATTACCGAGGCATAAAAAAATATAGAGTGTACTAGGAt                                                                        <  2:4178854/126‑1 (MQ=255)
                                                         ttATATAATGTATAATTCATTATGTGATAATGCCAATCGCTAAGAAAAAAAAAGAGTCATCCGCTAGGTGGAAAAAAAAAAATGAAAATCATTACCGAGGCATAAAAAAATATAGAGTGTACTAGGAt                                                                        <  2:3779347/128‑1 (MQ=255)
                                                         ttATATAATGTATAATTCATTATGTGATAATGCCAATCGCTAAGAAAAAAAAAGAGTCATCCGCTAGGTGG‑‑AAAAAAAAATGAAAATCATTACCGAGGCATAAAAAAATATAGAGTGTACTAGGATCg                                                                      <  1:1546261/128‑1 (MQ=255)
                                                           atataATGTATAATTCATTATGTGATAATGCCAATCGCTAAGAAAAAAAAAGAGTCATCCGCTAGGTGGAAAAAAAAAATGAAAATCATTACCGAGGCATAAAAAAATATAGAGTGTACTAGGATCGc                                                                     <  1:506221/128‑1 (MQ=255)
                                                             ataATGTATTATTCATTATGTGATAATGCCTATGGTTAATAAAAAAAAAGAGTCATCCGCTAGGTGGAAAAAAAAAATGAAAATCATTACCGAGGCATAAAAAAATATAGAGTGTACTAGGATCGCtt                                                                   <  1:2675429/128‑1 (MQ=255)
                                                              taATGTATAATTCATTATTTGATAATGACAATATCTAAGAAAAAAAAAGAGTCATCCGCTATGTGGAAAAAAAAAATGAAAATCATTACCGAGGCATAAAAAAATATAGAGTGTACTAGGATCGCTTg                                                                  <  2:2688949/128‑1 (MQ=255)
                                                              taATGTATAATTCATTATGTGATAATGCCAATCGCTAAGAAAAAAAAAGAGTCATCCGCTAGGTGGAAAAAAAAAATGAAAATCATTACCGAGGCATAAAAAAATATAGAGTATACTATGATCGCttt                                                                  >  2:3043124/1‑127 (MQ=255)
                                                                aTGTATAATTCATTATGTGATAATGCCAATCGCTAATAAAAAAAAAGAGTAATCCGCTAGGTGGAAAAAAAAAATGAAAATCATTACCGAGGCATAAAAAAATATAGAGTGTACTAGGATCGCTTGcc                                                                <  1:2826920/128‑1 (MQ=255)
                                                                  gTATAATTAATTATGTTATAATGCCAATCGCTTAGAAAAAAAAAGAGTCATCCGCTAGGTGGAAAAAAAAAATGAAAATCATTACCGAGGCATAAAAAAATATAGAGTGTACTAGGATCGCTTGCCTg                                                              <  1:3096474/128‑1 (MQ=255)
                                                                     taATTCATTATGTGATAATGCCAATCGCTAAGAAAAAAAAAGAGTCATCCGCTAGGTGGAAAAAAAAAATGAAAATCATTACCGAGGCATAATAAAAAATAGATTGTACTAGGATCGCTTGCGTGTag                                                           >  2:4351928/1‑127 (MQ=255)
                                                                        ttCATTATGTGATAATGCCAATCGCTAAGAAAAAAAAAGAGTCATCCGCTAGGTGGAAAAAAAAAATGAAAATCATTACCGAGGCATAAAAAAATATAGAGTGTACTAGGATCGCTTGCCTGTAACtt                                                        <  2:1583139/128‑1 (MQ=255)
                                                                             taagtgcaaacgCCAATCGCTATGAAAAAAAAAGAGTTATCCGATAGGTGGAAAAAAAAAATGAAAATCATTACCGAGGCATAAAAAAATATAGAGTGTACTAGGATCGCTTGCCTGTAACTTACAcg                                                   <  1:2065007/117‑1 (MQ=255)
                                                                               tgtgATACTTACAATCGCTAAGAAAAAAAAAGAGTCATCCGCTAGGTGGAAAAAAAAAATGAAAATCATTACCGAGGCATAAAAAAATATAGAGTGTACTCGGATCGCTTGCCTGTAACTTACAcgcg                                                 <  2:2809063/128‑1 (MQ=255)
                                                                               tgtgATAATGCCAATCGCTAAGAAAAAAAAAGAGTCATCCGCTAGGTGGAAAAAAAAAATGAAAATCATTACCGAGGCATAAAAAAATATAGAGTGTACTAGGATCGCTTGCCTGTAACTTACAcgcg                                                 <  2:2253898/128‑1 (MQ=255)
                                                                                tttatatTGCCAATCTCAAAGAAATAAAAAGAGTCATCAGCTAGGTGGAAAAAAAAAATGAAAATCATTACCGAGGCATAAAAAAATATAGAGTGTACTAGGATCGCTTGCCTGTAACTTACACgcgc                                                <  2:3466007/125‑1 (MQ=255)
                                                                                gtgATAATGCCAATCGCTAAGAAAAAAAAAGAGTCATCCGCTAGGTGGAAAAAAAAAATGAAAATCATTACCGAGGCATAAAAAAATATAGAGTGTACTAGGATCGCTTGCCTGTAACTTACACgcgc                                                <  1:3939455/128‑1 (MQ=255)
                                                                                gtgATAATGCCAATCGCTAAGAAAAAAAAAGAGTCATCCGCTAGGTGGAAAAAAAAAACGAAAATCATTACCGAGGCATAAAAAAATATAGAGTGTACTAGGATCGCTTGCCTGTAACTTACACgcgc                                                <  2:1087706/128‑1 (MQ=255)
                                                                                gtgATAATGCCAATAGCTAATAAAAAAAAAGAGTCATCCGCTAGGTGGAAAAAAAAAATGAAAATCATTACCGAGGCATAAAAAAATATAGAGTGTACTAGGATCGCTTGCCTGTAACTTACACgcgc                                                <  1:3381805/128‑1 (MQ=255)
                                                                                 tgATAATGCCAATCGCTAAGAAAAAAAAAGAGTCATCCGCTAGGTGGAAAAAAAAAAATGAAAATCATTACCGAGGCATAAAAAAATATAGAGTGTACTAGGATCACTTGCCTGTAACTTCCACgcgc                                                >  2:2138638/1‑128 (MQ=255)
                                                                                   aTAATGCCAATCGCTAAGAAAAAAAAAGAGTCAACCGCTAGGTGGAAAAAAAAAATGAAAATCATTACCGAGCAATAAAAAAATATAGAGTGTACTAGGATCCCTTGCCTGTAACATAAAAGCGACTc                                             >  1:694077/1‑128 (MQ=255)
                                                                                    tAATGCCAATCGCTAAGAAAAAAAATGAGTCATCCGCTAGGTGGAAAAAAAAAATGAAAATCATTACCGAGGCATAAAAAAATATAGAGTGTACTAGGATCGCTTGCCTGTAACTTACACGCGCCTCg                                            <  2:2316332/128‑1 (MQ=255)
                                                                                     aaTGCCAATCGCTAAGAAAAAAAAAGAGTCATCCGCTAGGTGGAAAAAAAAAATGAAAATCATTACCGAGGCATAAAAAAATATTGAGAGTACTAGGATCGCTTGCCTGTAACTGACACGCGCCTCGt                                           >  2:1143154/1‑128 (MQ=255)
                                                                                     aaTGCCAATCGCTAAGAAAAAAAAAGAGTCATCCGCTAGGTGGAAAAAAAAAATGAAAATCATTACCGAGGCAGAAAAAATTTTATAGTGTACTAGGAACGCTTTCCTGTAACTTTCAcgccacgcgg                                           >  2:1523666/1‑121 (MQ=255)
                                                                                       tGCCAATCGCTAAGAAAAAAAAAGAGTCATCCGCTAGGTGGAAAAAAAAAATGAAAATCATTACCGAGGCATAAAAAAATATATAGTGTACTAGGATCGCTTGCCTGTAACTTACACGCGCCTCGTAt                                         >  1:1978325/1‑128 (MQ=255)
                                                                                       tGCCAATCGCTAAGAAAAAAAAAGAGTCATCCGCTAGGTGGAAAAAAAAAATGAAAATCATTACCGAGGCATAAAAAAATATATAGTGTACTAGGATCGATTGCCTGTACCTTACCCGCGCCTCATag                                         >  1:3999113/1‑127 (MQ=255)
                                                                                         ccAATCGCTAAGAAAAAAAAAGAGTCATCCGCTAGGTGGAAAAAAAAAATGAAAATCATTACCGAGGCATAAAAAAATATAGAGTGTACTAGGATCCCATGCCTGTACCTTACACCCGCcgcgagaga                                       >  1:3181636/1‑123 (MQ=255)
                                                                                         ccAATCGCTAAGAAAAAAAAAGAGTCATCCGCTAGGTGG‑‑‑AAAAAAAATGAAAATCATTACCGAGGCATAAATAAATTTAGATTGTACTAGGATCGCTTGACTGAACGTTACCCGCTCCTCGTATCttt                                     >  1:911057/1‑128 (MQ=255)
                                                                                          cAATCGCTAAGAAAAAAAAAGAGTCATCCGCTAGGTGGAAAAAAAAAATGAAAATCATTACCGAGGCATAAAAAAATATAGAGTGTACTAGGATCGCTTGCCTGGAACTTACACGCGACTCGTAGCta                                      >  2:2763452/1‑127 (MQ=255)
                                                                                           aaTCGCTAAGAAAAAAAAAGAGTCATCCGCTAGGTGGAAAAAAAAAATGAAAATCATTACCGAGGCATAAAAAAATATAGAGTGTACTAGGATCGCTTGCCTGTAACTTACACGCGCCTCGTATCttt                                     <  1:1419719/128‑1 (MQ=255)
                                                                                            aTCGCTAAGAAAAAAAAAGAGTCATCCGCTAGGTGGAAAAAAAAAATGAAAATCATTACCGAGGCATAAACAAAGATAGAGGGTACTAGGATCGCTTGCCTGTCCCTAACACCCGCCACGGAtgtgtt                                    >  2:3110442/1‑123 (MQ=255)
                                                                                            aTCGCTAAGAAAAAAAAAGAGTCATCCGCTAGGTGGAAAAAAAAAATGAAAATCATTACCGAGGCATAAAAAAATTTAGAGGGTACTAGGCTCGCTTGCCTGTAACATACCCGCGCCTCGtctcgtgt                                    >  1:4404826/1‑124 (MQ=255)
                                                                                              cgataataaAAAAAAAGATTCATCCGCTTGGTGGAAAAAAAAAATGAAAATCATTACCGAGGCATAAAAAAATATAGAGTGTACTAGGATCGCTTGCCTGTAACTTACACGCGCCTCGTATCTTTTaa                                  <  2:2415907/125‑1 (MQ=255)
                                                                                               gCTAAGAAAAAAAAAGAGTCATCCGCTAGGTGGAAAAAAAAAAATGAAAATCATTACCGAGGCATAAAAAAATATAGAGTGTACTAGGATCGCTTGCCTGTAACTTACACGCGCCTCGTATTTTTTaa                                  >  2:3556123/1‑128 (MQ=255)
                                                                                                cTAAGAAAAAAAAAAGAGTCATCCGCTAGGTGGAAAAAAAAAATGAAAATCATTACAGAGGCTTAAAAAAATTTAGAGTGTAATAGGATCGATTGCCTGTAAATTAAACGAGCAGGGTATGTTTAAat                                 >  1:3733027/1‑128 (MQ=255)
                                                                                                cTAAGAAAAAAAAAGAGTCATCCGCTAGGTGGAAAAAAAAAATTAAAATCATTACCGAGGCATAAAAAAAAATATATTGTAATATGTACGCATAACTGTAAATTACACGCGACACGTTTCTTTTAatg                                >  1:1841978/1‑128 (MQ=255)
                                                                                                cTAAGAAAAAAAAAGAGTCATCCGCTAGGTGGAAAAAAAAAATGAAAATCATTACCGAGGCATAAAAAAATATAGAGTGTACTAGGATCGCTTGCCTGTAACTTACACGCGCCTCGTATCTTTTAatg                                <  1:1186048/128‑1 (MQ=255)
                                                                                                  aaGAAAAAAAAAGAGTCATCCGCTAGGTGGAAAAAAAAAATGAAAATCATTACCGAGGCATAAAAAAATATAGAGTGTACTAGGATCGCTTGCCTGTAACTTACACGCGCCTCGTATCTTTTAatgat                              <  2:3874807/128‑1 (MQ=255)
                                                                                                       aaaaaaatCAGTCATCCGATATGTGG‑‑AAAAAAAAATGAAAATCATTACCGAGGCATAAAAAAATATTCAGTGTACTAGGATCGCTTGCCTGTAACTTACACGCGCCTCTTATCTTTTAATGATGGaat                         <  1:1260554/128‑1 (MQ=255)
                                                                                                       aaaaaaaaGAGTCATCCGCTAGGTGGAAAAAAAAAATGAAAATCATTACCGAGGCATAAAAAAATATAGAGTGTACTAGGATCGCTTGCATGTAACTTACACGCGCCTCGAATCTTTTAATGATGGaa                          >  2:1312854/1‑128 (MQ=255)
                                                                                                        aaaaaaaGAGTCATCCGCTAGGTGGAAAAAAAAAATGAAATTCATTACCGAGGCATAAAAAAATATAGAGTGTACTTGGATCGCTTGCGTGTAACTTCCACGCGCCTCGGAGCTGTTAAGGCTGGcat                         >  2:1444224/1‑128 (MQ=255)
                                                                                                          aaaaaGCGGCACCCGCTAGTTGGAAAAAAACCATGAATATCATTACCGAGGCATAAAAAAATATAGAGTGTACTAGGATCGCTTGCCTGTAACTTTCCCGCGCCTCGTATCTTTTAATGATGGaataa                       <  1:2927918/128‑1 (MQ=255)
                                                                                                           aaaaGAGTCATCTGCTATGTGGAAAAAAAAAATGAAAATCATTACCGAGGCATAAAAAAATATAGAGTGTACTAGGATCGCTTGCCTGTAACTTACACGCGCCTCGTATCTTTTAATGATGGaataat                      <  2:1775030/128‑1 (MQ=255)
                                                                                                              tgcggcaaccGCGAGGGGGAAAAAAAAAATGAAAATCATTACCGAGGCATAAAAAAATATAGAGTGTACTAGGATCGGTTGCCTGTAACTTACACGCGCCTCGTATCTTTTTATGATGGAATAATTTg                   <  2:941072/120‑1 (MQ=255)
                                                                                                              agagTCATCCGCTAGGTGGAAAAAAAAAATTAAAATCATTACCGGGGCATAAAAAAATATAGAGTGTACAAGGTACGCTTGCCTGTAACTTACACGCGCCACCTTTCTTTTAATGTAGGAATTATTTg                   >  1:2373935/1‑128 (MQ=255)
                                                                                                              agagTCATCCGCTAGGTGGAAAAAAAAAATGAAAATCATTACCGAGGCATAAAAAAATATAGAGTGTACTAGGATCGCTTGCCTGTAACTTACACGCGCCTCGTATCTTTTAATGATGGAATAATTTg                   >  1:2153351/1‑128 (MQ=255)
                                                                                                               gagTCATCCGCTAGGTGGAAAAAAAAAATGAAAATCATTACCGAGGCATAAAAAAATATATAGTGTACTAGGATCGCTTGCAAGTAACTTACACGCGCCTCGTATCTTTTAATGATGGAATAATTTgg                  >  1:355760/1‑128 (MQ=255)
                                                                                                                agTCATCCGCTAGGTGGAAAAAAAAAATGAAAATCATTACCGAGGCATTAAAAAATATAGAGTGTACTAGGATCGATTGACTGTAACTTACACGCGCCTCGTATCAATTATTGCTGGATTAATTTggg                 >  1:4318405/1‑128 (MQ=255)
                                                                                                                  tCATCCGCTAGGTGGAAAAAAAAAATGAAAATCATTACCGAGGCATAAAAAAATATAGAGTGTACTAGGATCGCTTGCCTGTAACTTACACGCGCCTCGTAGCTTTTAATGATGGAATATTTTGGGat               >  2:1899900/1‑127 (MQ=255)
                                                                                                                    atactcTATGTGGAAAAAAAAAATGAATATCATTAACGAGGCATAAATAAATATAGAGTGTACTAGGATCGCTTGACTGTAACTTACACGCGCCTCGTATCTTTTAATGATGGAATAATTTGGGAAtt             <  2:1221986/123‑1 (MQ=255)
                                                                                                                    aTCCGCTAGGTGGAAAAAAAAAATGAAAATCATTACCGAGGCATAAAAAAATATAGAGTGTACTAGGATCGCTTGCCTGTAACTTACACGCGCCTCGTATCTTTTAATGATGGAATAATTTGGGAAtt             <  2:1662997/128‑1 (MQ=255)
                                                                                                                      ccGCTAGGTGGAAAAAAAAAAATGAAAATCATTACCGAGGCATAAAAAAATATATAGTGTACTAGGATCGCTTGCATGTAACTTACACGCGCCTCGTATCTTTTAATTATGGATTAATTTGGTAtttt            >  1:3833158/1‑128 (MQ=255)
                                                                                                                        tcTAGGTTGAAAAAAAAAATGATAATCATTACCGAGGCATAAAAAAATATAGAGTGTACTAGGATCGCTTGCCTGTAACTTACACGCGCCTCGTATCTTTTAATGATGGAATAATTTGGGAATTTAct         <  2:1840567/127‑1 (MQ=255)
                                                                                                                                 |                                                                                                                              
CTTCGTTTATCTTGCCTGCTCATTTTTTAGTATATTCTTCGAAGAAATCACATTACTTTATATAATGTATAATTCATTATGTGATAATGCCAATCGCTAAGAAAAAAAAAGAGTCATCCGCTAGGTGGAAAAAAAAAAATGAAAATCATTACCGAGGCATAAAAAAATATAGAGTGTACTAGGATCGCTTGCCTGTAACTTACACGCGCCTCGTATCTTTTAATGATGGAATAATTTGGGAATTTACTCTGTGTT  >  CP016816/19385‑19639

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: