Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 420,157 2 bp→TT intergenic (‑440/+402) JCVSYN2_01895 ← / ← JCVSYN2_01900 30S ribosomal protein S10/tRNA‑Thr

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816420,1570AT99.6% 8928.3 / NA 2825intergenic (‑440/+403)JCVSYN2_01895/JCVSYN2_0190030S ribosomal protein S10/tRNA‑Thr
Reads supporting (aligned to +/- strand):  ref base A (5/7);  new base T (1508/1302);  total (1513/1312)
Fisher's exact test for biased strand distribution p-value = 5.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP016816420,1580AT98.5% 8617.7 / NA 2827intergenic (‑441/+402)JCVSYN2_01895/JCVSYN2_0190030S ribosomal protein S10/tRNA‑Thr
Reads supporting (aligned to +/- strand):  ref base A (4/39);  new base T (1509/1275);  total (1513/1314)
Fisher's exact test for biased strand distribution p-value = 9.68e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 2926 total aligned reads displayed.

AAATTAAATTCATTCATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTAAAATTAAAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTC‑‑G‑‑TTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTAAACGTATAT  >  CP016816/420031‑420284
                                                                                                                               ||                                                                                                                                   
       attcattcATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATttttaatttta                                                                                                                              >  1:7868512/1‑125 (MQ=255)
       attcattcATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATttttaatttta                                                                                                                              >  2:3146303/1‑125 (MQ=255)
        ttcattcaTACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATttttaattttaa                                                                                                                             <  1:1009341/128‑5 (MQ=255)
          cattcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATa                                                                                                                           <  1:6004179/128‑1 (MQ=255)
          cattcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATa                                                                                                                           <  2:1964891/128‑1 (MQ=255)
           attcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTtaataa                                                                                                                          >  1:23611/1‑128 (MQ=255)
            ttcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAAc                                                                                                                         >  2:4183975/1‑128 (MQ=255)
            ttcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAAc                                                                                                                         >  1:973714/1‑128 (MQ=255)
            ttcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAAc                                                                                                                         <  2:7100882/128‑1 (MQ=255)
            ttcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGATGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAAc                                                                                                                         >  2:2108133/1‑128 (MQ=255)
             tcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACt                                                                                                                        >  1:6777509/1‑128 (MQ=255)
                tACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGaa                                                                                                                     <  1:2822699/128‑1 (MQ=255)
                  cTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAatt                                                                                                                   <  1:7145103/128‑1 (MQ=255)
                   atttAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAatta                                                                                                                  <  1:1670766/127‑1 (MQ=255)
                     ttAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAattatt                                                                                                                >  1:6639601/1‑128 (MQ=255)
                     ttAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAattatt                                                                                                                <  1:7568926/128‑1 (MQ=255)
                     ttAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTAATAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAattatt                                                                                                                >  1:5049374/1‑128 (MQ=255)
                      tAGTCTCATAAATCTAAGTATCTAAAATTATTTTATCAATTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTAttt                                                                                                               <  2:5656267/128‑1 (MQ=255)
                       aGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTAtttt                                                                                                              >  2:7336023/1‑128 (MQ=255)
                        gTCTCATAAATCTAAGTTTCATAATTTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATATCTGAATTAttttt                                                                                                             <  1:3317060/128‑1 (MQ=255)
                           tcATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCaa                                                                                                          >  1:6131830/1‑128 (MQ=255)
                           tcATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCaa                                                                                                          >  2:492691/1‑128 (MQ=255)
                           taaTAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCaa                                                                                                          <  1:5885335/126‑1 (MQ=255)
                           taaTAAATCTAAGTTGCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCaa                                                                                                          <  2:5826781/126‑1 (MQ=255)
                             aTAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAAt                                                                                                        <  1:766229/128‑1 (MQ=255)
                             aTAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAAt                                                                                                        >  2:5248348/1‑128 (MQ=255)
                             aTAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAAt                                                                                                        <  2:3432709/128‑1 (MQ=255)
                              tAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTTAATTATTTTTCAAAtt                                                                                                       >  2:5380271/1‑128 (MQ=255)
                              tAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAAtt                                                                                                       <  2:6121500/128‑1 (MQ=255)
                               aaaTCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTg                                                                                                      <  2:365798/128‑1 (MQ=255)
                               aaaTCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTg                                                                                                      <  1:193931/128‑1 (MQ=255)
                                aaTCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGt                                                                                                     <  2:160630/128‑1 (MQ=255)
                                 aTCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGtt                                                                                                    <  1:2657967/128‑1 (MQ=255)
                                   cTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTaa                                                                                                  >  2:7246548/1‑128 (MQ=255)
                                       gTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTTAatta                                                                                              >  1:1544445/1‑128 (MQ=255)
                                        tttCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAattat                                                                                             <  2:6743182/128‑1 (MQ=255)
                                        tttCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAattat                                                                                             <  1:7737959/128‑1 (MQ=255)
                                         ttCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTATCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAattatt                                                                                            >  2:6620936/1‑128 (MQ=255)
                                         ttCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAACATTTTTAATTTTAATATCTGAATTATTTGTCAAATTGTTAAattatt                                                                                            >  1:7281709/1‑128 (MQ=255)
                                         ttCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTTAATTATTTTTCAAATTTTTAAattatt                                                                                            >  2:4617256/1‑128 (MQ=255)
                                         ttCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGtt                                                                                                    <  1:5275046/120‑1 (MQ=255)
                                         ttCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGt                                                                                                     >  2:4760160/1‑119 (MQ=255)
                                         ttCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAattatt                                                                                            >  1:545767/1‑128 (MQ=255)
                                          tCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAttt                                                                                           <  2:1756680/128‑1 (MQ=255)
                                           cTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAtttt                                                                                          >  1:1803292/1‑128 (MQ=255)
                                           cTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAtttt                                                                                          >  2:5042880/1‑128 (MQ=255)
                                           cTAAAATTATTTTATCATTTAAAAAAATAATACTTATATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAtttt                                                                                          <  2:6492329/128‑1 (MQ=255)
                                            tAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTc                                                                                         <  2:406451/128‑1 (MQ=255)
                                            tAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTc                                                                                         <  1:4228469/128‑1 (MQ=255)
                                             aaaaTTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCa                                                                                        >  2:1313283/1‑128 (MQ=255)
                                             aaaaTTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCa                                                                                        >  2:2889427/1‑128 (MQ=255)
                                                attattTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAAt                                                                                     <  2:2258591/128‑1 (MQ=255)
                                                  tattTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCa                                                                                   <  2:1127511/128‑1 (MQ=255)
                                                  tattTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCa                                                                                   <  1:3146303/128‑1 (MQ=255)
                                                    ttttATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCAtt                                                                                 <  1:3726372/128‑1 (MQ=255)
                                                    ttttATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCAtt                                                                                 >  2:5504019/1‑128 (MQ=255)
                                                     tttATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTa                                                                                <  2:2754149/128‑1 (MQ=255)
                                                     aaaataaTTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCTAATTGTTAAATTATTTTCAAATCATTa                                                                                >  1:4085394/7‑128 (MQ=255)
                                                       aaTCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATc                                                                              <  2:1871134/127‑1 (MQ=255)
                                                        aTCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCt                                                                             >  2:5977701/1‑128 (MQ=255)
                                                          cATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAatt                                                                                                                   <  2:6981866/88‑1 (MQ=255)
                                                          cATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTAt                                                                           <  2:5135209/128‑1 (MQ=255)
                                                               aaaaaaaTAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATc                                                                      >  1:6436790/1‑128 (MQ=255)
                                                                  aaaaTAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAAt                                                                  >  1:6314970/1‑128 (MQ=255)
                                                                  aaaaTAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAAt                                                                  >  1:7413832/1‑128 (MQ=255)
                                                                     ataataCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAAtttt                                                               >  2:3947260/1‑128 (MQ=255)
                                                                     ataataCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAAtttt                                                               <  1:1333702/128‑1 (MQ=255)
                                                                      taataCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTa                                                              >  1:2207432/1‑128 (MQ=255)
                                                                          aCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCt                                                          >  2:3272750/1‑128 (MQ=255)
                                                                          aCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCt                                                          >  1:5716662/1‑128 (MQ=255)
                                                                             tttATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATTCTC‑‑G‑‑t                                                   >  2:4349844/1‑128 (MQ=255)
                                                                             tttAATATTTTAAATATTATAAAACAACTACCACTATTTTAAAAAAATTTTAAATTTAATAACTGAATTATATTTCAAATTGTTAAATTAATTTCAAATCATTATCAATTAATCAATATTATGCCC‑‑G‑‑t                                                   <  1:298174/128‑1 (MQ=255)
                                                                              tttttatttttaataataGAAAAATACTATCAATTTTTTAAAAAATTTTTAATTTTAATAACTTAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATAAATTTTATGCTC‑‑G‑‑tt                                                  <  2:3527880/116‑1 (MQ=255)
                                                                                attaGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTC‑‑T‑‑tttt                                                >  2:4504154/1‑128 (MQ=255)
                                                                                     ttGTTAAATTATAAAAATACTATCACTTTTTTAAAAAATTTTTAATTTTAAAAACTTAATTATTTTTCAAATTGTTAAATTATTTTAAAATCATTATCTATTAATCAATTTTATGCTC‑‑G‑‑ttttttttt                                           <  2:2479268/128‑1 (MQ=255)
                                                                                      ttatatatTATAAAAATACTATCACTTTTTTAAAAAATTTTTAATTTTAATAAATTAAATATTTTTAAAATTTTTAAATTATTTTCAAAACATTATCAATTAAACAATTTTATGCTC‑‑G‑‑tttttttttt                                          <  2:6870036/126‑1 (MQ=255)
                                                                                        atatatTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTC‑‑G‑‑tttttttttttt                                        >  1:6542342/1‑128 (MQ=255)
                                                                                          atatTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTC‑‑G‑‑tttttttttttttt                                      >  1:6910483/1‑128 (MQ=255)
                                                                                          atatTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTC‑‑G‑‑tttttttttttttt                                      >  1:3949197/1‑128 (MQ=255)
                                                                                            atTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTGAAATCATTATCTATTAATAAATTTTATGCTC‑‑T‑‑ttattttttttttttt                                    >  1:837232/1‑128 (MQ=255)
                                                                                                 aaaaATAATAGAACATTTTTAAAAAATTTTTAAATATTAAAAATTAATTATTTTTAAATTTGTTAAAATATTTTTAAATAATTTTCTATTAATAAATTTAATTCTC‑‑G‑‑TTTTTTTTTTTTTTTTTACaa                               <  1:3559947/128‑1 (MQ=255)
                                                                                                  aaaCTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTC‑‑G‑‑TTTTTTTTTTTTTTTTACaaaa                             >  1:4605826/1‑128 (MQ=255)
                                                                                                  aaaCTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATATTATGCTC‑‑G‑‑TTTTTTTTTTTTTTTTTAaaaa                              >  2:772573/1‑128 (MQ=255)
                                                                                                   aaatattataaattttttAAAAAATTTTTATTTTTAATAAATTTATTTTTTTTAAAATTTTTTAATTATTTTAAAATAAATTTATTTTAATAAATTTTTTTTTTT‑‑T‑‑TTTTTTTTTTTTTTTTTACaaa                              <  1:2074732/116‑1 (MQ=255)
                                                                                                     aaaatataaattttttTAAAAATTTTTAATTTTTATAACTTTATTTTTTTTAAAATTTTTTAATTATTTTAAAATCTTTATCTATTATTAAATTTTATGCTC‑‑G‑‑TTTTTTTTTTTTTTTTTACAAAAat                           <  1:5169366/118‑1 (MQ=255)
                                                                                                        ctaGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTC‑‑G‑‑ttttttatttttttttttttaaaaaaaa                        >  2:2641520/1‑117 (MQ=255)
                                                                                                        ctaGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTC‑‑G‑‑TTTTTTTTTTTTTTTTAAAAAATATaaa                      >  2:6401358/1‑127 (MQ=255)
                                                                                                          aGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATTCTC‑‑G‑‑TTTTTTTTTTTTTTTTAAAAAAATATAatt                     >  2:1496610/1‑128 (MQ=255)
                                                                                                          aGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTC‑‑G‑‑TTTTTTTTTTTTTTTTTAAAAAAAaataat                      >  2:3023968/1‑128 (MQ=255)
                                                                                                          aGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGATC‑‑GTTTTTTTTTTTTTTTTTTAAAAAAATATaa                       >  2:7484834/1‑128 (MQ=255)
                                                                                                           gCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTC‑‑G‑‑TTTTTTTTTTTTTTTTTAAAAAAATATAatt                     >  2:873236/1‑128 (MQ=255)
                                                                                                           gCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTC‑‑G‑‑TTTTTTTTTTTTTTTTTAAAAAAATATAatt                     >  1:701228/1‑128 (MQ=255)
                                                                                                             aCTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTC‑‑G‑‑TTTTTTTTTTTTTTTTTAAAAAAATATAatttt                   >  1:4426870/1‑127 (MQ=255)
                                                                                                                 tttttAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTC‑‑GTTTTTTTTTTTTTTTTTTAAAAAAATATAatttatt                 >  2:6272250/1‑128 (MQ=255)
                                                                                                                 tttttAAAAAATTTTTAATTTTAATAAATTAATTATTTTTTAAATTTTTTAATTTTTTTATAATAATTATATATTTATTAATTTTTTTCTCTTT‑‑TTTTTTTTTTTTTTTTTACAAAAATATAatttat                  <  2:3787806/128‑1 (MQ=255)
                                                                                                                    ttAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTC‑‑G‑‑TTTTTTTTTTTTTTTTAAAAAAATAATAAtttattttat             >  2:7898600/1‑128 (MQ=255)
                                                                                                                     tAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTC‑‑G‑‑TTTTTTTTTTTTTTTATTCAAAAAAATAatttatttttta            >  1:2502193/1‑124 (MQ=255)
                                                                                                                     tAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTC‑‑G‑‑TTTTTTTTTTTTTTTTACAAAAATATAATTTATTttatta           >  2:7608127/1‑128 (MQ=255)
                                                                                                                         aaaTTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTC‑‑G‑‑TTTTTTTTTTTTTTTTAAAAAAATAAAATTTATTTTTTTAAAct       >  1:4859005/1‑127 (MQ=255)
                                                                                                                            ttttAAATTTTAATAAATTAATTTTTTTAAAATTTTTAAAATTTTTTTAAATAATTATATTATAATAAATTTTATTTTT‑‑GTTTTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTAAACGt      <  2:2356407/128‑1 (MQ=255)
                                                                                                                               ||                                                                                                                                   
AAATTAAATTCATTCATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTAAAATTAAAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTC‑‑G‑‑TTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTAAACGTATAT  >  CP016816/420031‑420284

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: