BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP016816	20,023	2 bp→TA	intergenic (‑744/‑392)	JCVSYN2_00065 ← / → JCVSYN2_00070	hypothetical protein/tetracycline resistance ribosomal protection protein Tet(M)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP016816	20,023	0	A	T	99.6%	9936.9 / NA	3341	intergenic (‑744/‑393)	JCVSYN2_00065/JCVSYN2_00070	hypothetical protein/tetracycline resistance ribosomal protection protein Tet(M)
Reads supporting (aligned to +/- strand): ref base A (10/4); new base T (1784/1541); total (1796/1545)
Fisher's exact test for biased strand distribution p-value = 2.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	CP016816	20,024	0	T	A	99.5%	10056.9 / NA	3338	intergenic (‑745/‑392)	JCVSYN2_00065/JCVSYN2_00070	hypothetical protein/tetracycline resistance ribosomal protection protein Tet(M)
Reads supporting (aligned to +/- strand): ref base T (10/7); new base A (1783/1537); total (1793/1545)
Fisher's exact test for biased strand distribution p-value = 8.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 3381 total aligned reads displayed.

AGCAGGAAGAGCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATAATTTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGAAAAAACAAAAAAAGATTA  >  CP016816/19900‑20149

||

        gatcaacaaaaaaGGGATTATTTGTTGGCGATCCACCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTaaa                                                                                                                    <  2:7349902/119‑1 (MQ=255)

         agCAAGATAAAAGGTAGTTTTTGTTGGCGATCCCCCTAGAGTCTTTTACATATTCGGAAAACAAAAACTATTTTTTCTTTAATTTGTTTTTTTAATTTCTATTTTTAATTTATATATTTATTTTaaaa                                                                                                                   >  1:7441674/1‑128 (MQ=255)

         agCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTaaaa                                                                                                                   >  2:3664517/1‑128 (MQ=255)

         agCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTATCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTaaaa                                                                                                                   >  2:7488207/1‑128 (MQ=255)

          gCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTaaaaa                                                                                                                  >  1:376333/1‑128 (MQ=255)

              gATAAAATGTAGTATTTGTTGGCGATACCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAAttt                                                                                                              <  2:4656448/128‑1 (MQ=255)

              gATAAAAGGTAGTATTTGTTGGAGATCACCATAGAGTCTTTTAGATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAAttt                                                                                                              <  2:1799135/128‑1 (MQ=255)

                tAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTaa                                                                                                            <  1:4441400/128‑1 (MQ=255)

                tAAAAGGTAGTATTTGGTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTaa                                                                                                            <  1:7824882/128‑1 (MQ=255)

                  aaaGGGAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTATTTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTTAAAAATTTAaat                                                                                                          >  1:4016584/1‑128 (MQ=255)

                    aGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAaatta                                                                                                        <  1:4740324/128‑1 (MQ=255)

                     ggTAGTATTTGTTGGCGATACCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAAATTATATATTTATATAAAAAAATTTAtattat                                                                                                       >  2:1408370/1‑128 (MQ=255)

                       tagtaTTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAaattataa                                                                                                     >  2:3828014/1‑128 (MQ=255)

                       tagtaTTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAaattataa                                                                                                     <  1:4290239/128‑1 (MQ=255)

                        agtaTTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAaattataat                                                                                                    >  2:5971411/1‑128 (MQ=255)

                         gtaTTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAaattataatt                                                                                                   >  1:6404903/1‑128 (MQ=255)

                         gtaTTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAaattataatt                                                                                                   >  2:4988123/1‑128 (MQ=255)

                          taTTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAaattataatta                                                                                                  >  1:3359555/1‑128 (MQ=255)

                           aTTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAaattataattat                                                                                                 >  2:1233145/1‑128 (MQ=255)

                            tttGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAattatt                                                                                                <  1:2411737/128‑1 (MQ=255)

                            agatttgTCGATCCCACTAGAGTCTTTTACATATTATGAATACAAATACTATTTTTTCTTTAATTTATTTTTTTTCTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAattatt                                                                                                <  2:2649172/124‑1 (MQ=255)

                             ttgttgGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTAttt                                                                                               >  1:2236950/1‑128 (MQ=255)

                             ttgttgGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTAttt                                                                                               <  1:5862130/128‑1 (MQ=255)

                                ttgGCGATCCAACTAGAGTCTTTTACATCTTAGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTta                                                                                            <  1:693805/128‑1 (MQ=255)

                                    cGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCGATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGc                                                                                        >  2:6668675/1‑128 (MQ=255)

                                    cGATCCCACTAGAGTCTTATAAATCTTCGGAAAACAAAATCTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGc                                                                                        <  2:3075931/128‑1 (MQ=255)

                                     gATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCa                                                                                       <  1:1656501/128‑1 (MQ=255)

                                     gATACCGCTAGAGTCTTTTACATCTTAGTAAAACAAAAAATATTTTTTATTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCa                                                                                       <  2:4329977/128‑1 (MQ=255)

                                       tCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACg                                                                                     >  1:819983/1‑128 (MQ=255)

                                        cccccTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGt                                                                                    <  1:6279170/128‑1 (MQ=255)

                                         ctcatAGAGTCTTTTTCATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTCTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTg                                                                                   <  2:6940616/124‑1 (MQ=255)

                                         ccccTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTg                                                                                   >  1:1121862/1‑128 (MQ=255)

                                           ccTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACTTGAt                                                                                 >  2:7684516/1‑128 (MQ=255)

                                           ccTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGAt                                                                                 <  2:2782457/128‑1 (MQ=255)

                                           ccTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGAt                                                                                 <  1:1398087/128‑1 (MQ=255)

                                            cTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATa                                                                                >  1:3867775/1‑128 (MQ=255)

                                             tAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATaa                                                                               <  1:7717229/128‑1 (MQ=255)

                                             tAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATaa                                                                               <  2:7207338/128‑1 (MQ=255)

                                                agTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATGGCACGTGATAATTa                                                                            >  1:7232759/1‑128 (MQ=255)

                                                    ttttACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAg                                                                        >  2:1718310/1‑128 (MQ=255)

                                                      ttACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATATAACTTTATATATAAAAGtt                                                                      >  1:5755220/1‑128 (MQ=255)

                                                      ttACATCATCGGAAAACAAAAACTATTTAATCTTTAATTTATTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGtt                                                                      <  1:5476686/128‑1 (MQ=255)

                                                       gaCATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTa                                                                     <  2:1594147/127‑1 (MQ=255)

                                                         cATCTTCTGAAAACATAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGt                                                                   <  2:6810616/128‑1 (MQ=255)

                                                         cATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTTATAATTAAAAGTTAGt                                                                   >  1:5367181/1‑128 (MQ=255)

                                                         cATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGt                                                                   >  2:7820960/1‑128 (MQ=255)

                                                         cATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTATTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATATTTATAGAACTTGATAATTAAAAGATAGt                                                                   >  1:5610151/1‑128 (MQ=255)

                                                           tCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGa                                                                 <  2:3369674/128‑1 (MQ=255)

                                                            cTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGaa                                                                <  1:4124137/128‑1 (MQ=255)

                                                               cGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACaa                                                             >  2:3981337/1‑128 (MQ=255)

                                                               aggAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTA‑TTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAg                                                            <  2:4447256/127‑1 (MQ=255)

                                                                ggAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACa                                                              >  2:5583263/1‑126 (MQ=255)

                                                                ggAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAg                                                            >  2:1098987/1‑128 (MQ=255)

                                                                  aaaaCAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGaa                                                          <  1:361927/128‑1 (MQ=255)

                                                                  aaaaCAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGATAGTGAACAAGaa                                                          >  1:5008671/1‑128 (MQ=255)

                                                                    aaCAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAg                                                                                         <  1:7065421/95‑1 (MQ=255)

                                                                      cAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACa                                                      >  1:4871234/1‑128 (MQ=255)

                                                                      cAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTA‑TTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAg                                                     >  1:6110913/1‑128 (MQ=255)

                                                                       aaaaaCTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAg                                                     <  1:1267272/128‑1 (MQ=255)

                                                                        aaaaCTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACTGt                                                    >  2:3502463/1‑128 (MQ=255)

                                                                        aaaaCTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGt                                                    <  2:5698243/128‑1 (MQ=255)

                                                                        aaaaCTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGt                                                    <  1:2684046/128‑1 (MQ=255)

                                                                            cTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAg                                                >  1:6700289/1‑128 (MQ=255)

                                                                            cTATTTTTTCTTTAATTTCTATTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAg                                                <  1:7573375/128‑1 (MQ=255)

                                                                              aTTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCa                                              >  1:3494417/1‑128 (MQ=255)

                                                                              aTTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCa                                              <  1:1002158/128‑1 (MQ=255)

                                                                               ttttttCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCAc                                             >  2:2090030/1‑128 (MQ=255)

                                                                                  tttCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTGTAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAg                                          <  2:3201874/128‑1 (MQ=255)

                                                                                    tCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGtt                                        >  1:5997424/1‑128 (MQ=255)

                                                                                     cTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGttt                                       >  1:2805357/1‑128 (MQ=255)

                                                                                     cTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGttt                                       >  2:6244325/1‑128 (MQ=255)

                                                                                     cTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGttt                                       >  2:5314038/1‑128 (MQ=255)

                                                                                     cTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGttt                                       >  2:5430984/1‑128 (MQ=255)

                                                                                     cTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATAATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGttt                                       <  2:4140236/128‑1 (MQ=255)

                                                                                      tttAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTc                                      >  2:6534699/1‑128 (MQ=255)

                                                                                      tttAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTc                                      >  1:1935343/1‑128 (MQ=255)

                                                                                        tAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTg                                    <  1:1539710/128‑1 (MQ=255)

                                                                                              cTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCaa                              <  1:1773249/128‑1 (MQ=255)

                                                                                               tttttttACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTaaaa                                                                         <  2:2309513/84‑1 (MQ=255)

                                                                                               tttttttACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCaaa                             >  1:3112712/1‑128 (MQ=255)

                                                                                               tttttttACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCaaa                             <  1:253758/128‑1 (MQ=255)

                                                                                                tattttACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTTAACAAGAAAACAGTGAAGCAACAGTTTCTGAACCaaaa                            <  1:2959263/126‑1 (MQ=255)

                                                                                                  ttttACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAaaga                          >  1:4598888/1‑128 (MQ=255)

                                                                                                    ttACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAaagaag                        >  2:2933217/1‑128 (MQ=255)

                                                                                                     tACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAagaaga                       >  1:2061915/1‑128 (MQ=255)

                                                                                                     tACTTTCTATTTTTAATTTAATTATTTATATTAAAAAATTTTAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAagaaga                       <  1:986586/128‑1 (MQ=255)

                                                                                                      aCTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGAc                      >  2:5829902/1‑128 (MQ=255)

                                                                                                      aCTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGAc                      >  1:6814038/1‑128 (MQ=255)

                                                                                                      aCTTTCTATTTTTAAATTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTTTTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGAc                      >  1:5174220/1‑128 (MQ=255)

                                                                                                       cTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACg                     >  2:803734/1‑128 (MQ=255)

                                                                                                         ttCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAATCAGTGAAGAACCAGTTTCTGAACCAATAGAAGACGaa                   >  1:6560615/1‑128 (MQ=255)

                                                                                                         ttCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGTCGaa                   >  1:7349184/1‑128 (MQ=255)

                                                                                                         ttCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGaa                   >  2:607710/1‑128 (MQ=255)

                                                                                                         ttCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGaa                   >  2:6403756/1‑128 (MQ=255)

                                                                                                             aTTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGaaaaaa               <  2:4862749/128‑1 (MQ=255)

                                                                                                              tttttAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGAAAAAAc              <  2:1932568/128‑1 (MQ=255)

                                                                                                               ttttAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGAAAAAACa             >  2:514358/1‑128 (MQ=255)

                                                                                                                 ttAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGAAAAAACaaa           >  1:3243596/1‑128 (MQ=255)

                                                                                                                  tAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGAAAAAACaaaa          >  2:7053186/1‑128 (MQ=255)

                                                                                                                      ttATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGAAAAAACAAAAAAAg      >  2:2473471/1‑128 (MQ=255)

||

AGCAGGAAGAGCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATAATTTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGAAAAAACAAAAAAAGATTA  >  CP016816/19900‑20149

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`