Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 479,854 C→T G1225S (GGT→AGT)  JCVSYN2_02215 ← DNA‑directed RNA polymerase subunit beta

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816479,8540CT99.7% 11976.6 / NA 3803G1225S (GGT→AGT) JCVSYN2_02215DNA‑directed RNA polymerase subunit beta
Reads supporting (aligned to +/- strand):  ref base C (0/1);  major base T (1827/1958);  minor base A (8/5);  total (1839/1964)
Fisher's exact test for biased strand distribution p-value = 4.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 3803 total aligned reads displayed.

AAATTCAGCATCAACATCTTCATCATCAATATATAAAGTATCTGTTTTTACTAAAATTTCATCATCTTCATAATTATCTGCGTCAAAGTCTTTTTTATCATATGCATTAATTACTGATTTTTCACCAGTTTCATCAATCATATACATATTAAATCCTAGACCCATAATTTCTTTTGATAACACGTTAAATGATTCTGGAATTCCAGGTTCAGGAATTCTTTTTGATCTAACAATTGCTTCATAAGTTTTT  >  CP016816/479729‑479978
                                                                                                                             |                                                                                                                            
 aaTTCAGCATCAACATCTTCATCATCAATATATAAAGTATCTGTTTTTACTAAAATTTCATCATCTTCATAATTATCTGCGTCAAAGTCTTTTTTATCATATGCATTAATTACTGATTTTTAACTAGt                                                                                                                           >  2:4388136/1‑128 (MQ=255)
  tttCAGCATATAGATCTTCATAATAATTATATAAAGTATCTGTTTTAACTAAAATTTCATCATCTTCATAATTATCTGCGTCAAAGTCTTTTTTATCATATGCATTAATTACTGATTTTTCACTAGtt                                                                                                                          <  1:3513455/127‑1 (MQ=255)
  aTTCAGCATCGACATCTTCATCATCAATATATAAAGTATCTTTTTTTACTAAAATTTCATCATCTTCATAATTATCTGCGTCAAAGTCTTTATTATCATATGCATTAATTACTTATTTTTCACTAGtt                                                                                                                          >  1:1344923/1‑128 (MQ=255)
  aTTCAGCATCAACTTCTTCATCATCAATATATAAAGTATCTGTTTTTACTAAAATTTCATCATCTTCATAATTATCTGCGTCAAAGTCTTTTTTATCATATGCATTAATTACTGATTTTTCACTAGtt                                                                                                                          <  2:65359/128‑1 (MQ=255)
  aTTCAGCATCAACATCTTCATCATCAATATATAAAGTATCTGTTTTTACTAAAATTTCATCATCTTCATAATTATCTGCGTCAAAGTCTTTTTTATCATATGCATTAATTACTGATTTTTCACTAGtt                                                                                                                          <  1:6226962/128‑1 (MQ=255)
  aTTCAGCATCAACATCTTCATCATCAATATATAAAGTATCTGTTTTTACTAAAATTTCATCATCTTCATAATTATCTGCGTCAAAGTCTTTTTTATCATATGCATTAATTACTGATTTTTCACTAGtt                                                                                                                          <  1:2196003/128‑1 (MQ=255)
  aTTCAGCATCAACATCTTCATCATCAATATATAAAGTATCTGTTTTTACTAAAATTTCATCATCTTCATAATTATCTGCGTCAAAGTCTTTTTTATCATATGCATTAATTACTGATTTTTCACTAGtt                                                                                                                          <  1:4789127/128‑1 (MQ=255)
   ttCAGCATCAACATCTTCATCATCAATATATAAAGTATCTGTTTTTACTAAAATTTCATCATCTTCATAATTATCTGCGTCAAAGTCTTTTTTATCATATGCATTAATTACTGATTTTTCACTAGttt                                                                                                                         <  1:7665802/128‑1 (MQ=255)
   ttCAGCATCAACATCTTCATCATCAATATATAAAGTATCTGTTTTTACTAAAATTTCATCATCTTCATAATTATCTGCGTCAAAGTCTTTTTTATCATATGCATTAATTACTGATTTTTCACTAGttt                                                                                                                         <  2:6023915/128‑1 (MQ=255)
    tCAGCATCAACATCTTCATCATCAATATATAAAGTATCTGTTTTTACTAAAATTTCATCATCTTCATAATTATCTGCGTCAAAGTCTTTTTTATCATATGCATTAATTACTGATTTTTCACTAGTTtc                                                                                                                        <  2:5491523/128‑1 (MQ=255)
    tCAGCATCAACATCTTCATCATCAATATATAAAGTATCTGTTTTTACTAAAATTTCATCATCTTCATAATTATCTGCGTCAAAGTCTTTTTTATCATATGCATTAATTACTGATTTTTCACTAGTTtc                                                                                                                        <  1:1648754/128‑1 (MQ=255)
    tCAGCATCAACATCTTCATCATCAATATATAAAGTATCTGTTTTTACTAAAATTTCATCATCTTCATAATTATCTGCGTCAAAGTCTTTTTTATCATATGCATTAATTACTGATTTTTCACTAGTTtc                                                                                                                        >  1:2733839/1‑128 (MQ=255)
        cATCAACATCTTCATCATCAATATATAAAGTATCTGTTTTTACTAAAATTTCATCATCTTCATAATTATCTGCGTCAAAGTCTTTTTTATCATATGCATTAATTACTGATTTTTCACTAGTTtcatca                                                                                                                    <  2:3311939/128‑1 (MQ=255)
          tCAACATCTTCATCATCAATATATAAAGTATCTGTTTTTACTAAAATTTCATCATCTTCATAATTATCTGCGTCAAAGTCTTTTTTATCATATTCATTAATTACTGATTTTTCACTAGTTTCatcaat                                                                                                                  >  1:5877483/1‑128 (MQ=255)
          tCAACATCTTCATCATCAATATATAAAGTATCTGTTTTTACTAAAATTTCATCATCTTCATAATTATCTGCGTCAAAGTCTTTTTTATCATATGCATTAATTACTGATTTTTCACTAGTTTCatcaat                                                                                                                  <  2:4718198/128‑1 (MQ=255)
           cAACATCTTCATCATCATTATATAAAGTATCTGTTTTTACTAAAATTTCATCATCTTCATAATTATCTGAGTCAAAGTCTTTTTTATCATATGCATTAATTACTGAATTTTCACTAGTTTCataaatc                                                                                                                 >  2:5004921/1‑128 (MQ=255)
           cAACATCTTCATCATCAATATATAAAGTATCTGTTTTTACTAAAATTTCATCATCTTCATAATTATCTGCGTCAAAGTCTTTTTTATCGTATGCATTAATTACTGATTTTTCACTAGTTTCatcaatc                                                                                                                 >  2:176410/1‑128 (MQ=255)
           cAACATCTTCATCATCAATATATAAAGTATCTGTTTTTACTAAAATTTCATCATCTTCATAATTATCTGCGTCAAAGTCTTTTTTATCATATGCATTAATTACTGATTTTTCACTAGTTTCatcaatc                                                                                                                 >  1:450434/1‑128 (MQ=255)
           cAACATCTTCATCATCAATATATAAAGTATCTGTTTTTACTAAAATTTCATCATCTTCATAATTATCTGCGTCAAAGTCTTTTTTATCATATGCATTAATTACTGATTTTTCACTAGTTTCatcaatc                                                                                                                 >  2:455734/1‑128 (MQ=255)
           cAACATCTTCATCATCAATATATAAAGTATCTGTTTTTACTAAAATTTCATCATCTTCATAATTATCTGCGTCAAAGTCTTTTTTATCATATGCATTAATTACTGATTTTTCACTAGTTTCatcaatc                                                                                                                 >  1:3020435/1‑128 (MQ=255)
            aaCATCTTCATCATCAATATATAAAGTATCTGTTTTTACTAAAATTTCATCATCTTCATAATTATCTGCGTCAAAGTCTTTTTTATCATATGCATTAATTACTGATTTTTCACTAGTTTCatcaatca                                                                                                                <  2:5348053/128‑1 (MQ=255)
            aaCATCTTCATCATCAATATATAAAGTATCTGTTTTTACTAAAATTTCATCATCTTCATAATTATCTGCGTCAAAGTCTTTTTTATCATATGCATTAATTACTGATTTTTCACTAGTTTCatcaatc                                                                                                                 >  2:7860059/1‑127 (MQ=255)
              cATCTTCATCATCAATATATAAAGTATCTGTTTTTACTAAAATTTCATCATCTTCATAATTATCTGCGTCAAAGTCTTTTTTATCATATGCATTAATTACTGATTTTTCACTAGTTTCATCAATCata                                                                                                              <  1:33428/128‑1 (MQ=255)
                tCTTCATCATCAATATATAAAGTATCTGTTTTTACTAAAATTTCATCATCTTCATAATTATCTGCGTCAAAGTCTTTTTTATCATATGCATTAATTACTGATTTTTCACTAGTTTCATCAATCAtata                                                                                                            >  1:6385647/1‑128 (MQ=255)
                  ttCATCATCAATATATAAAGTATCTGTTTTTACTAAAATTTCATCATCTTCATAATTATCTGCGTCAAAGTCTTTTTTATCATATGCATTAATTACTGATTTTTCACTAGTTTCATCAATCATATACa                                                                                                          >  1:2454667/1‑128 (MQ=255)
                   tcatcatcaATATATAAAGTATCTGTTTTTACTAAAATTTCATCATCTTCATAATTATCTGCGTCAAAGTCTTTTTTATCATATGCATTAATTACTGATTTTTCACTAGTTTCATCAATCATATACat                                                                                                         >  2:7560265/1‑128 (MQ=255)
                    catcatcaATATATAAAGTATCTGTTTTTACTAAAATTTCATCATCTTCATAATTATCTGCGTCAAAGTCTTTTTTATCATATGCATTAATTACTGATTTTTCACTAGTTTCATCAATCATATACata                                                                                                        >  2:1241368/1‑128 (MQ=255)
                      tcatcaATATATAAAGTATCTGTTTTTACTAAAATTTCATCATCTTCATAATTATCTGCGTCAAAGTCTTTTTTATCATATGCATTAATTACTGATTTTTCACTAGTTTCATCAATCATATACATAtt                                                                                                      >  1:1002465/1‑128 (MQ=255)
                       catcaATATATAAAGTATCTGTTTTTACTAAAATTTCATCATCTTCATAATTATCTGCGTCAAAGTCTTTTTTATCATATGCATTAATTACTGATTTTTCACTAGTTTCATCAATCATATACATATTa                                                                                                     <  2:6159020/128‑1 (MQ=255)
                          caAAATATCAAGTATCTGATTTTACTAAAATTTCATCATCTTCATAATTATCTGCGTCAAAGTCTTTTTTATCATATGCATTAATTACTGATTTTTCACTAGTTTCATCAATCATATACATATTAAAt                                                                                                  <  1:982914/128‑1 (MQ=255)
                           aaTATATAAAGTATCTGTTTTTACTAAAATTTCATCATCTTCATAATTATCTGCGTCAAAGTCTTTTTTATCATATGCATTAATTACTGATTTTTCACTAGTTTCATCAATCATATACATATTAAATc                                                                                                 >  1:1744385/1‑128 (MQ=255)
                              aaataAAGTATCTGTTTTTACTAAAATTTTATCATCTTCATAATTATCTGCGTCAAAGTCTTTTTTATCATATGCATTATTTACTGATTTTTCACTAGTTTAATCAATCATATACATATTAAATCCTa                                                                                              <  2:7166985/126‑1 (MQ=255)
                               tataAAGTATCTGTTTTTACTAAAATTTCATCATCTTCATAATTATCTGCGTCAAAGTCTTTTTTATCATATGCATTAATTACTGATTTTTCACTAGTTTCATCAATCATATACATATTAAATCCTAg                                                                                             <  2:5187271/128‑1 (MQ=255)
                                ataAAGTATCTGTTTTTACTAAAATTTCATCATCTTCATAATTATCTGCGTCAAAGTCTTTTTTATCATATGCATTAATTACTGATTTTTCACTAGTTTCATCAATCATATACATATTAAATCCTAGa                                                                                            <  1:5532081/128‑1 (MQ=255)
                                ataAAGTATCTGTTTTTACTAAAATTTCATCATCTTCATAATTATCTGCGTCAAAGTCTTTTTTATCATATGCATTAATTACTGATTTTTCACTAGTTTCATCAATCATATACATATTAAATCCTAGa                                                                                            <  2:6809177/128‑1 (MQ=255)
                                ataAAGTATCTGGATTTACTAAAATTTCATCATATTCATAATTATCTGCGTCAAAGTCTTTTTTATCATATGCATTAATTACTGATTTTTCACTAGTTTCATCAATCATATACATATTAAATCCTAGa                                                                                            <  1:7373554/128‑1 (MQ=255)
                                 taAAGTATCTGTTTTTACTAAAATTTCATCATCTTCATAATTATCTGCGTCAAAGTCTTTTTTATCATATGCATTAATTACTGATTTTTCACTAGTTTCATCAATCATATACATATTAAATCCTAGAc                                                                                           <  2:1106611/128‑1 (MQ=255)
                                 taAAGTATCTGTTTTTACTAAAATTTCATCATCTTCATAATTATCTGCGTCAAAGTCTTTTTTATCATATGCATTAATTACTGATTTTTCACTAGTTTCATCAATCATATACATATTAAATCCTAGAc                                                                                           >  1:821146/1‑128 (MQ=255)
                                 taAAGTATCTGTTGTTACTAATATTTCATCATATTCATAATTATATGCGTCAAAGTCTTTTTTATCATATGCATTAATTACTGATTTTTCACTAGTTTCATCAATCATATACATATTAAATCCTAGAc                                                                                           <  2:1909942/128‑1 (MQ=255)
                                  taaGTATCTGTTTTTACTAAAATTTCATGATCTTCATAATTATCTGCGTCAAAGTCTTTTTTATCATATGCATTTATTACTGATTTTTCACTAGTTTCATCAATCATATACATATTAAATCCTAGAcc                                                                                          <  1:5401150/127‑1 (MQ=255)
                                   aaGTATATGTTTATAATAAAATTTCATCATCTTCATAATTATCTGAGTCAAAGTCTTTTTTATCATATGCATTAATTACTGATTTTTCACTAGTTTCATCAATCATATACATATTAAATCCTAGAccc                                                                                         <  1:2068222/128‑1 (MQ=255)
                                      tATCTGTTTTTACTAAAATTTCATCATCTTCATAATTATCTGCGTCAAAGTCTTTTTTATCATATGCATTAATTACTGATTTTTCACTAGTTTCATCAATCATATACATATTAAATCCTAGACCCATa                                                                                      >  2:2900028/1‑128 (MQ=255)
                                       aTCTGTTTTTACTAAAATTTCATCATCTTCATAATTATCTGCGTCAAAGTCTTTTTTATCATATGCATTAATTACTGATTTTTCACTAGTTTCATCAATCATATACATATTAAATCCTAGACCCATaa                                                                                     >  2:829552/1‑128 (MQ=255)
                                        tCTGTTTTTACTAAAATTTCATCATCTTCTTAATTATCTGAGTCAAAGTCTTTTTTATCATATGCATTAATTACTGATTTTTCACTAGTTTCATCAATCATATACATATTAAATCCTAGACCCATAAt                                                                                    <  2:7044339/128‑1 (MQ=255)
                                          tGTTTTTACTAAAATTTCATCATCTTCATAATTATCTGCGTCAAAGTCTTTTTTATCATATGCATTAATTACTGATTTTTCACTAGTTTCATCAATCATATACATATTAAATCCTAGACCCATAAttt                                                                                  >  2:1352950/1‑128 (MQ=255)
                                              tttACTAAAATTTCATCATCTTCATAATTATCTGCGTCAAAGTCTTTTTTATCATATGCATTAATTACTGATTTTTCACTAGTTTCATCAATCATATACATATTAAATCCTAGACCCATAATTTCttt                                                                              >  1:6938467/1‑128 (MQ=255)
                                                tACTAAAATTTCATCATCTTCATAATTATCTGCGTCAAAGTCTTTTTTATCATATGCATTAATTACTGATTTTTCACTAGTTTCATCAATCATATACATATTAAATCCTAGACCCATAATTTCTTTTg                                                                            >  1:3704712/1‑128 (MQ=255)
                                                      aaTTTCATCATCTTCATAATTATCTGCGTCAAAGTCTTTTTTATCATATGCATTAATTACTGATTTTTCACTAGTTTCATCAATCATATACATATTAAATCCTAGACCCATAATTTCTTTTGATAaca                                                                      <  2:382624/128‑1 (MQ=255)
                                                       aTTTCATCATCTTCATAATTATCTGCGTCAAAGTCTTTTTTATCATATGCATTAATTACTGATTTTTCACTAGTTTCATCAATCATATACATAtt                                                                                                      >  1:7502229/1‑95 (MQ=255)
                                                        tttCATCATCTTCATAATTATCTGCGTCAAAGTCTTTTTTATCATATGCATTAATTACTGATTTTTCACTAGTTTCATCAATCATATACATATTAAATCCTAGACCCATAATTTCTTTTGATAACACg                                                                    <  2:5633006/128‑1 (MQ=255)
                                                          tcatcaTCTTCATAATTATCTGCGTCAAAGTCTTTTTTATCATATGCATTAATTACTGATTTTTCACTAGTTTCATCAATCATATACATATTAAATCCTAGACCCATAATTTCTTTTGATAACACGtt                                                                  >  2:2058458/1‑128 (MQ=255)
                                                          tcatcaTCTTCATAATTATCTGCGTCAAAGTCTTTTTTATCATATGCATTAATTACTGATTTTTCACTAGTTTCATCAATCATATACATATTAAATACTAGACCCATAATATCTTTTGATAACACGtt                                                                  >  2:1627375/1‑128 (MQ=255)
                                                           catcatCTTCATAATTATCTGCGTCAAAGTCTTTTTTATCATATGCATTAATTACTGATTTTTCACTAGTTTCATCAATCATATACATATTAAATCCTAGACCCATAATTTCTTTTGATAACACGTTa                                                                 <  1:2900028/128‑1 (MQ=255)
                                                            atcatcTTCATAATTATCTGCGTCAAAGTCTTTTTTATCATATGCATTAATTACTGATTTATCACTAGTTTCATCAATCATATACATATTAAATCCTAGACCCATAATTTCTTTTGATAACACTTTaa                                                                >  1:148175/1‑128 (MQ=255)
                                                             tcatcTTCATAATTATCTGCGTCAAAGTCTTTTTTATCATATGCATTAATTACTGATTTTTCACTAGTTTCATCAATCATATACATATTAAATCCTAGACCCATAATTTCTTTTGATAACACGTTaaa                                                               >  1:3181329/1‑128 (MQ=255)
                                                              catcTTCATAATTATCTGCGTCAAAGTCTTTTTTATCATATGCATTAATTACTGATTTTTCACTAGTTTCATCAATCATATACATATTAAATCCTAGACCCATAATTTCTTTTGATAACACGTTAAAt                                                              <  2:4082608/128‑1 (MQ=255)
                                                                tattattAATTATGTGAGTCTAAGTCTTTTTTATGATAATCATTAATTACTGATTTTTTACTAGTTTCATCAATCATATACATATTAAATCCTAGCCCCATAATTTCTTTTGATAACACGTTAAATGa                                                            <  2:3031654/122‑1 (MQ=255)
                                                                 cTTCATAATTATCTGCGTCAAAGTCTTTTTTATCATATGCATTAATTACTGATTTTTCACTAGTTTCATCAATCATATTCATATTAAATCCTACACACATAATTTCTTTAGATAACAAGTTAAAAGaa                                                           >  1:1919710/1‑127 (MQ=255)
                                                                 cTTCATAATTATCTGCGTCAAAGTCTTTTTTATCATATGCATTAATTACTGATTTTTCACTAGTTTCATCAATCATATACATATTAAATCCTATACCCATAATTTCTTTTGATAAAACGTTAAATGAt                                                           >  1:4223103/1‑128 (MQ=255)
                                                                 cTTCATAATTATCTGCGTCAAAGTCTTTTTTATCATATGCATTAATTACTGATTTTTCACTAGTTTCATCAATCATATACATATTAAATCCTAGACCCATAATTTCTTTTGATAACACGTTAAATGAt                                                           >  2:2493015/1‑128 (MQ=255)
                                                                 cTTCATAATTATCTGCGTCAAAGTCTTTTTTATCATATGCATTAATTACTGATTTTTCACTAGTTTCATCAATCATATACATATTAAATCCTAGACCCATAATTTCTTTTGATAACACGTAAAATGAt                                                           >  2:2620618/1‑128 (MQ=255)
                                                                 cTTCATAATTATCTGCGTCAAAGTCTTTTTTATCATATGCATTAATTACTGATTTTTCACTAGTTTCATCAATCATATACATATTAAATCCTAGACCCAAAATTTCTTTTGATAACACTTTAAATGAt                                                           >  1:4379465/1‑128 (MQ=255)
                                                                  ttCATAATTATCTGCGTCAAAGTCTTTTTTATCATATGCATTAATTACTGATTTTTCACTAGTTTCATCAATCATATACATATTAAATCCTAGACCCATAATTTCTTTTGATAACACGTTAAATGAtt                                                          >  2:6324201/1‑128 (MQ=255)
                                                                   tCATAATTATCTGCGTCAAAGTCTTTTTTATCATATGCATTAATTACTGATTTTTCACTAGTTTCATCAATCATATACATATTAAATCCTAGACCCATAATTTCTTTTGATAACACGTTAAATGCTTc                                                         >  1:1202642/1‑128 (MQ=255)
                                                                    cATAATTATCTGCGTCAAAGTCTTTTTTATCATATGCATTAATTACTGATTTTTCACTAGTTTCATCAATCATATACATATTAAATCCTAGACCCATAAt                                                                                    >  2:5887750/1‑100 (MQ=255)
                                                                     aTAATTATCTGCGTCAAAGTCTTTTTTATCATATGCATTAATTCCTGATTTTTCACTAGTTTCATCAATCATATACATATTAAATCCTAGACCCATAATTTCTTTTGATAACACGTTAAATGATTCTg                                                       <  1:5634083/128‑1 (MQ=255)
                                                                      tAATTATCTGCGTCAAAGTCTTTTTTATCATATGCATTAATTACTGATTTTTCACTAGTTTCATCAATCATATACATATTAAATCCTAGACCCATAATTTCTTTTGATAACACGTTAAATGATTCTgg                                                      <  2:2791998/128‑1 (MQ=255)
                                                                            tCTGCGTCAAAGTCTTTTTTATCATATGCATTAATTACTGATTTATCACTAGTTTAATCAATCATATACATATTAAATCCTAGACCAATAATTTCTTTTGATAACACGTTAAATGATTCTGGAATTcc                                                <  1:5199066/128‑1 (MQ=255)
                                                                                cttccaaGTCTTTTTTATCATATGCATTAATTACTGATTTTTCACTATTTTAATCAATCATATACATATTAAATCCTACACCCATAATTTCTTTTGATAACACGTTAAATGATTCTGGAATTCCAGGt                                            <  1:4104637/123‑1 (MQ=255)
                                                                                cGTCAAAGTCTTTTTTATCATATGCATTACTTACTGATTTTTCACTAGTTTCATCAATCATATACATATTAAATCCTAGACCCATAATTTCTTTTGATAACACGTTAAATGATTCTGGAATTCCAGGt                                            <  2:3134790/128‑1 (MQ=255)
                                                                                cGTCAAAGTCTTTTTTATCATATGCATTAATTACTGATTTTTCACTAGTTTCATCAATCATATACATATTAAATCCTAGACCTATAATTTCTTTTGATAACACGTTAAATGATTCTGGAATTCCAGGt                                            >  1:6532608/1‑128 (MQ=255)
                                                                                cGTCAAAGTCTTTTTTATCATATGCATTAATTACTGATTTTTCACTAGTTTCATCAATCATATACATATTAAATCCTAGACCCATAATTTCTTTTGATAACACGTTAAATGATTCTGGAATTCCAGGt                                            <  2:7300226/128‑1 (MQ=255)
                                                                                cGTCAAAGTCTTTTTTATCATATGCATTAATTACTGATTTTTCACTAGTTTCATCAATCATATACATATTAAATCCTAGACCCATAATTTCTTTTGATAACACGTTAAATGATTCTGGAATTCCAGGt                                            >  1:7104455/1‑128 (MQ=255)
                                                                                cGTCAAAGTCTTTTTTATCATATGCATTAATTACTGATTTTTCACTAGTTTCATCAATCATATACATATTAAATCCTAGACCCATAATTTCTTTTGATAACACGTTAAATGATTCTGGAATTCCAGGt                                            <  1:7845613/128‑1 (MQ=255)
                                                                                  tCAAAGTCTTTTTTATCATATGCATTAATTACTCATTTTTCACTAGATTCATCCATCATATACATATAACATCCTAGACCCATAATTTCTTTTGAGACCACGATCAATGAATCTCGAATTCCAGGTTc                                          >  1:4950020/1‑128 (MQ=255)
                                                                                   cAAAGTCTTTTTTATCATATGCATTAATTACTGATTTTTCACTAGTTTCATCAATCATATACATATTAAATCCTAGACCCATAATTTCTTTTGATAACACGTTAAATGATTCTGGAATTcctgaacct                                         <  1:232238/128‑8 (MQ=255)
                                                                                   cAAAGTCTTTTTTATCATATGCATTAATTACTGATTTTTCACTAGTTTCATCAATCATATACATATTAAATCCTAGACCCATAATTTCTTTTGATAACACGTTAAATGATTCTGGAATTCCAGGTTCa                                         <  2:6931488/128‑1 (MQ=255)
                                                                                    aaaGTCTTATTTATCATATGCATTAATTACTGATTTTTCACTAGTTTCATCAATCATATACATATTAAATCCTAGACCCATAATTTCTTTTGATAACACGTTAAATGATTCTGGAATTCCAGGTTCAg                                        <  1:5758885/128‑1 (MQ=255)
                                                                                        tcatttttATCAAATGCATTAATTACTGATTTTTCACTAGATTCATCAATCATATACATATTAAATCCTAGACCCATACTTTCTTTTGATAACACGTTAAATGATTCTGGAATTCCAGGTTCAGGAAt                                    <  2:5768324/125‑1 (MQ=255)
                                                                                         cTTTTTTATCATATGCATTAATTACTGATTTTTCACTAGTTTCATCAATCATATACATATTAAATCCTAGACCCATAATTTCTTTTGATAACACGTTAAATGATTCTGGAATTCCAGGTTCAGGAAtt                                   <  1:3885019/128‑1 (MQ=255)
                                                                                            ttttATCATATGCAGTAATTACTGATTTTTCACTAGTTTCATCAATCATATACATATTAAATCCTAGACCCATAATTTCTTTTGATAACACGTTAAATGATTCTGGAATTCCAGGTTCAGGAATTCtt                                <  2:3672852/128‑1 (MQ=255)
                                                                                              ttATCATATGCATTAATTACTGATTTTTCACTAGTTTCATCAATCATATACATATTAAATCCTAGACCCATAATTTCTTTTGATAACACGTTAAATGATTCTGGAATTCCAGGTTCAGGAATTCtttt                              >  2:7440988/1‑128 (MQ=255)
                                                                                               tATCATATGCATTAATTACTGATTTTTCACTAGTTTCATCAATCATATACATATTAAATCCTAGACCCATAATTTCTTTTGATAACACGTTAAATGATTCTGGAATTCCAGGTTCAGGAATTCttttt                             <  2:3955096/128‑1 (MQ=255)
                                                                                               tATCATATGCATTAATTACTGATTTTTCACTAGTTTCATCAATAATATACATATTAAATCATACACCCATAATTTCTTTTGATAACACGTTAAATGATTCTGGAATTCCAGGTTCAGGAAttaattat                             >  1:4641929/1‑122 (MQ=255)
                                                                                                aTCATATGCATTAATTACTGATTTTTCACTAGTTTCATCAATCATATACATATTAAATCCTAGACCCATAATTTCTTATGATAACACGTTAAATGATTCTGGAATTCCAGGTTCAGGAATTCTTTTTg                            >  1:3976130/1‑128 (MQ=255)
                                                                                                        cATTAATTACTGATTTTTCACTAGTTTCATCAATCATATACATATTAAATCCTAGACCCATAATTTCTTTTGATAACACGTTAAATGATTCTGGAATTCCAGGTTCAGGAATTCTTTTTGATCTAACa                    >  2:6661771/1‑128 (MQ=255)
                                                                                                            aattaCTGATTTTTCACTAGTTTCATCAATCATATACATATTAAATCCTAGACCCATAATTTCTTTTGATAACACGTTAAATGATTCTGGAATTCCAGGTTCAGGAATTCTTTTTGATCTAACAATTg                <  2:3443680/128‑1 (MQ=255)
                                                                                                            aattaCTGATTTTTCACTAGTTTCATCAATCATATACATATTAAATCCTAGACCCATAATTTCTTTTGATAACACGTTAAATGATTCTGGAATTCCAGGTTCAGGAATTCTTTTTGATCTAACAATTg                <  2:1488815/128‑1 (MQ=255)
                                                                                                            aattaCTGATTTTTCACTAGTTTCATCAATCATATACATATTAAATCCTAGACCCATAATTTCTTTTGATAACACGTTAAATGATTCTGGAATTCCAGGTTCAGGAATTCTTTTTGATCTAACAATTg                <  2:772642/128‑1 (MQ=255)
                                                                                                               taCTGATTTTTCACTAGTTTCATCAATCATATACATATTAAATCCTAGACCCATAATTTCTTTTGATAACACGTTAAATGATTCTGGAATTCCAGGTTCAGTAATTCTTTTTGATCTAACCAtatcta             >  1:5099885/1‑123 (MQ=255)
                                                                                                               taCTGATTTTTCACTAGTATCATCAATCATATACATATTAAATCCTAGACCCATAATTTCTTTTGATAACACGTTAAATGATTCTGGAATTCCAGGTTCAGGAATTCTTTTTGATCTAACAATTGCtt             <  2:4212540/128‑1 (MQ=255)
                                                                                                                aCTGATTTTTCACTAGTTTCATCAATCATATACATATTAAATCCTAGACCCATAATTTCTTTTGATAACACGTTAAATGATTCTGGAATTCCAGGTTCAGGAATTCTTTTTGATCTAACAATTGCTTc            <  2:485920/128‑1 (MQ=255)
                                                                                                                     tttttCACTAGTTTCATCAATCATATACATATTAAATCCTAGACCCATAATTTCTTTTGATAACACGTTAAATGATTCTGGAATTCCAGGTTCAGGAATTCTTTTTGATCTAACAATTGCTTCATAAg       >  2:2349404/1‑128 (MQ=255)
                                                                                                                     tttttCACTAGTTTCATCAATCATATACATATTAAATCCTAGACCCATAATTTCTTTTGATAACACGTTAAATGATGCTGGAATTCCAGGTTCAGGAATTCTTTTTGATCTAACAATTGCTTCATAAg       <  1:2577681/128‑1 (MQ=255)
                                                                                                                     tatttCACTAGTTTCATCAATCATATACTTATAATATCCTTGACACATAATTTCTTTTGATAACTCGTTAAATGATTCTGGAATTCCAGGTTCAGGAATTCTTTTTGATCTAACAATTGCTTCATAAg       <  1:7269567/126‑1 (MQ=255)
                                                                                                                      ttttCACTAGTTTCATCAATCATATACATATTAAATCCTAGACCCATAATTTCTTTTGATAACACGTTAAATGATTCTGGAATTCCAGGTTCAGGAATTCTTTTTGATCTAACAATTGCTTCATAAGt      >  1:6672863/1‑128 (MQ=255)
                                                                                                                        ttCACTAGTTTCATCAATCATATACATATTAAATCCTAGACCCATAATTTCTTTTGATAACACGTTAAATGATTCTGGAATTCCAGGTTCAGGAATTCTTTTTGATCTAACAATTGCTTCATAAGttt    <  1:6810259/128‑1 (MQ=255)
                                                                                                                        ttCACTAGTTTCATCAATCATATACATATTAAATCCTAGACCCATAATTTCTTTTGATAACACGTTAAATGATTCTGGAATTCCAGGTTCAGGAATTCTTTTTGATCTAACAATTGCTTCATAAGttt    <  1:6019463/128‑1 (MQ=255)
                                                                                                                        ttCACTAGTTTCATCAATCATATACATATTAAATCCTAGACCCATAATTTCTTTTGATAACACGTTAAATGATTCTGGAATTCCAGGTTCAGGAATTCTTTTTGATCTAACAATTGCTTCATAAGttt    >  2:2213798/1‑128 (MQ=255)
                                                                                                                        ttCACTAGTTTCATCAATCATATACATATTAAATCCTAGACCCATAATTTCTTTTGATAACACGTTAAATGATTCTGGAATTCCAGGTTCAGGAATTCTTTTTGATCTAACAATTGCTTCATAAGttt    >  2:3540989/1‑128 (MQ=255)
                                                                                                                             |                                                                                                                            
AAATTCAGCATCAACATCTTCATCATCAATATATAAAGTATCTGTTTTTACTAAAATTTCATCATCTTCATAATTATCTGCGTCAAAGTCTTTTTTATCATATGCATTAATTACTGATTTTTCACCAGTTTCATCAATCATATACATATTAAATCCTAGACCCATAATTTCTTTTGATAACACGTTAAATGATTCTGGAATTCCAGGTTCAGGAATTCTTTTTGATCTAACAATTGCTTCATAAGTTTTT  >  CP016816/479729‑479978

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑