BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP016816	258,095	T→C	F49L (TTT→CTT)	JCVSYN2_01095 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP016816	258,095	0	T	C	99.4%	5151.4 / NA	1388	F49L (TTT→CTT)	JCVSYN2_01095	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/1); major base C (737/637); minor base A (6/2); total (744/644)
Fisher's exact test for biased strand distribution p-value = 2.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1406 total aligned reads displayed.

AAACCTAAAAAAGCCCCCCCATTAACTATTGAAGAAATTAATGAAATAAAACAACACTCATCTTATGAAAAAAGTTATCTAAAAACTTTTAATAAATATAAAAAGAAAGTCGAGCATCGAATCTATTTTAAAACTAGTTTTTGATGAGATATTTTTATTATTGCTTTAGCTGCTTTAGCAAATACTATTACAACTGATTATTTTATTTTAGCAACTGGAGATACTGGATTATTTCCTGGTGGTACTGCTAC  >  CP016816/257969‑258219

  aacTAAAAAAGCCCCCCCATTAACTCTTGAAGAAATTAATGAAATAAAACAACACTCATCTTATGAAAAAAGTTATCTAAAAGCTTTAAATAAATATAAAAAGAAAGGCGAGCATCGAATCTATCTTa                                                                                                                           <  1:1506571/126‑1 (MQ=255)

  aCCTAAAAAAGCCCCCCCATTAACTATTGAAGAAATTAATGAAATAAAACAACACTCATCTTATGAAAAAAGTTATCTAAAAACTTTTAATAAATATAAAAAGAAAGTCGAGCATCGAATCTATCTTa                                                                                                                           <  2:1966829/128‑1 (MQ=255)

  aCCTAAAAAAGCCCCCCCATTAACTATTGAAGAAATTAATGAAATAAAACAACACTCATCTTATGAAAAAAGTTATCTAAAAACTTTTAATAAATATAAAAAGAAAGTCGAGCATCGAATCTATCTTa                                                                                                                           <  2:2467173/128‑1 (MQ=255)

      aaaaaaGCCCCCCCATTAACTATTGAAGAAATTAATGAAATAAAACAACACTCATCTTATGAAAAAAGTTATCTAAAAACTTTTAATAAATATAAAAAGAAAGTCGAGCCTCGAATCTATCTTAAAAc                                                                                                                       >  1:980198/1‑128 (MQ=255)

      aaaaaaGCCCCCCCATTAACTATTGAAGAAATTAATGAAATAAAACAACACTCATCTTATGAAAAAAGTTATCTAAAAACTTTTAATAAATATAAAAAGAAAGTCGAGCATCGAATCTATCTTAAAAc                                                                                                                       >  2:2783790/1‑128 (MQ=255)

       aaaaaGCCCCCCCATTAACTATTGAAGAAATTAATGAAATAAAACAACACTAATCTTATGAAAAAAGTTATCTAAAAACTTTTAATAAATATAAAAAGCAAGTCGATCATCGAATCTATCTTAAAACt                                                                                                                      >  2:2932987/1‑128 (MQ=255)

        aaaaGCCCCCCCATTAACTATTGAAGAAATTAATGAAATAAAACAACACTCATCTTATGAAAAAAGTTATCTAAAAACTTTTAATAAATATATAAAGAAAGTCGAGAATCGAATCTAAGTTAAACCTa                                                                                                                     >  1:1349665/1‑128 (MQ=255)

            gCCCCCCCATTAACTATTGAAGAAATTAATGAAATAAAACAACACTCATCTTATGAAAAAAGTTATCTAAAAACTTTTAATAAATATAAAAAGAAAGTCGAGCATCGAATCTATCTTAAAACTAGttt                                                                                                                 <  2:2541867/128‑1 (MQ=255)

            gCCCCCCCATTAACTATTGAAGAAATTAATGAAATAAAACAACACTCATCTTATGAAAAAAGTTATCTAAAAACTTTTAATAAATATAAAAAGAAAGTCGAGCATCGAATCGATCTTAAAACTAGttt                                                                                                                 >  2:2423485/1‑128 (MQ=255)

              ccccccATTAACTATTGAAGAAATTAATGAAATAAAACAACACTCATCTTATGAAAAAAGTTATCTATAAACTTTTAATAAATATAAAAAGAAAGTCGAGCATCGAATCTATCTTAAAACTAGttttt                                                                                                               <  2:532744/128‑1 (MQ=255)

               cccccATTAACTATTGAAGAAATTAATGAAATAAAACAACACTCATCTTATGAAAAAAGTTATCTAAAAACTTTTAATAAATATAAAAAGAAAGTCGAGCATCGAATCTATCTTAAAACTAGTTTTtg                                                                                                              <  1:1926545/128‑1 (MQ=255)

                ccccATTAACTATTGAAGAAATTAATGAAATAAAACAACACTCATCTTATGAAAAAAGTTATCTAAAAACTTTTAATAAATATAAAAAGAAAGTCGAGCATCGAATCTATCTTAAAACTAGTTTTtga                                                                                                             >  1:2388921/1‑128 (MQ=255)

                 cccAGTAACTATTGAAGAAATTAATGAAATAAAACAACACTCATCTTATGAAAAAAGTTATCTAAAAACTTTTAATAAATATAAAAAGAAAGTCGAGCATCGAAGCTATCTTAAAACTAGGTTGtgat                                                                                                            >  2:2898905/1‑128 (MQ=255)

                  ccATTAACTATTGAAGAAATTAATGAAATAAAACAACACTCATCTTATGAAAAAAGTTATCTAAAAACTTTTAATAAATATAAAAAGAAAGTCGAGCATCGAATCTATCTTAAAACTAGTTTTtgatg                                                                                                           >  1:2958730/1‑128 (MQ=255)

                  ccATTAACTATTGAAGAAATTAATGAAATAAAACAACACTCATCTTATGAAAAAAGTTATCTAAAAACTTTTAATAAATATAAAAAGAAAGTCGAGCATCGAATCTATCTTAAAACTAGTTTTtgatg                                                                                                           <  2:1847803/128‑1 (MQ=255)

                  ccATTAACTATTGAAGAAATTAATGAAATAAAACAACACTCATCTTATGAAAAAAGTTATCTAAAAACTTTTAATAAATATAAAAAGAAAGTCGAGCATCGAATCTATCTTAAAACGAGTATTttatg                                                                                                           >  2:1110764/1‑128 (MQ=255)

                   cATTAACTATTGAAGAAATTAATGAAATAAAACAACACTCATCTTATGAAAAAAGTTATCTAAAAACTTTTAATAAATATAAAAAGAAAGTCGAGCATCGAATCTATCTTAAAACTAGTTTTtgatga                                                                                                          >  1:2803148/1‑128 (MQ=255)

                   cATTAACTATTGAAGAAATTAATGAAATAAAACAACACTCATCTTATGAAAAAAGTTATCTAAAAACTTTTAATAAATATAAAAAGAAAGTCGAGCATCGAATCTATCTTAAAACTAGTTTTtgatga                                                                                                          >  2:3185382/1‑128 (MQ=255)

                    aTTAACTATTGAAGAAATTAATGAAATAAAACAACACTCATCTTATGAAAAAAGTTATCTAAAAACTTTTAATAAATATAAAAAGAAAGTCGAGCATCGAATCTATCTTAAAACTAGTTTTTGATgag                                                                                                         <  2:22709/128‑1 (MQ=255)

                      tAACTATTGAAGAAATTAATGAAATAAAACAACACTCATCTTATGAAAAAAGTTATCTAAAAACTTTTAATAAATATAAAAAGAAAGTCGAGCATCGAATCTATCTTAAAACTAGTTTTTGATGAGat                                                                                                       <  1:916874/128‑1 (MQ=255)

                        aCTATTGAAGAAATTAATGAAATAAAACAACACTCATCTTATGAAAAAAGTTATCTAAAAACTTTTAATAAATATAAAAAGAAAGTCGAGCATCGAATCTATCTTAAAACTAGTTTTTGATGAGatat                                                                                                     <  1:463716/128‑1 (MQ=255)

                        aCTATTGAAGAAATTAATGAAATAAAACAACACTCATCTTATGAAAAAAGTTATCTAAAAACTTTTAATAAATATAAAAAGAAAGTCGAGCATCCAATCTATCTTAAAACTACTTTTtgatgtgtttt                                                                                                     >  1:1724540/1‑122 (MQ=255)

                               aagaaATTAATGAAATAAAACAACACTCATCTTATGAAAAAAGTTATCTAAAAACTTTTAATAAATATAAAAAGAAAGTCGAGCATCGAATCTATCTAAAAACTATTTTTTGATGAGATATTTttatt                                                                                              >  1:576870/1‑128 (MQ=255)

                                agaaATTAATGAAATAAAACAACACTCATCTTATGAAAAAAGTTATCTAAAAACTTTTAATAAATATAAAAAGAAAGTCGAGCATCGAATCTATCTTAAAACTAGTTTTTGATGAGATATTTttatta                                                                                             >  2:1253356/1‑128 (MQ=255)

                                agaaATTAATGAAATAAAACAACACTCATCTTATGAAAAAAGTTATCTAAAAACTTTTAATAAATATAAAAAGAAAGTCGAGCATCGAATCTATCTTAAAACTAGTTTTTGATGAGATATTTttatta                                                                                             >  2:1608616/1‑128 (MQ=255)

                                  aaaTTAATGAAATAAAACAACACTCATCTTATGAAAAAAGTTATCTAAAAACTTTTAATAAATATAAAAAGAAAGTCGAGCATCTAATCTATCTTAAAACTAGTTTTTGATGAGATATTTTTattatt                                                                                           >  1:1862324/1‑128 (MQ=255)

                                     ttAATGAAATAAAACAACACTCATCTTATGAAAAAAGTTATCTAAAAACTTTTAATAAATATAAAAAGAAAGTCGAGCATCGAATCTATCTTAAAACTAGTTTTTGATGAGATAATTTTATTATtgct                                                                                        >  1:313445/1‑128 (MQ=255)

                                       aaTGAAATAAAACAACACTCATCTTATGAAAAAAGTTATCTAAAAACTTTTAATAAATATAAAAAGAAAGTCGAGCATCGAATCTATCTTAAAACTAGTTTTTGATGAGAAATTTTTATTATtgcttt                                                                                      >  2:1023795/1‑128 (MQ=255)

                                            aaacaaaCAACACACATCTTATTAAAAAAGATATCTAAACACTTTTAATAAAAATAAAAAGAAAGTCGAGCATCGAATCTATCTTAAAACTAGTTTTTGATGAGATATTTTTATTATtgctttagctg                                                                                 <  1:2007922/124‑1 (MQ=255)

                                             aTAAAACAACACTCATCTTATGAAAAAAGTTATCTAAAAACTTTTAATAAATATAAAAAGAAAGTCGAGCATCGAATCTATCTTAAAACTAGTTTTTGATGAGATATTTTTATTATtgctttagctgc                                                                                <  1:350784/128‑1 (MQ=255)

                                             aTAAAACAACACTCATCTTATGAAAAAAGTTATCTAAAAACTTTTAATAAATATAAAAAGAAAGTCGAGCATCGAATCTATCTTAAAACTAGTATTTGAGTAGATATTTTTATTATttctttagctcc                                                                                >  1:3204077/1‑126 (MQ=255)

                                             aTAAAACAACACTCATCTTATGAAAAAAGTAATCTAAAAACTTTTAATAAATATAAAAAGAAAGTCGAGCATCGAATCTATCTTAAAACTAGTTTTTGATGAGATATTTTTTTTATtgctttagctgc                                                                                <  2:1353950/128‑1 (MQ=255)

                                              tAAAACAACACTCATCTTATGAAAAAAGTTATCTAAAAACTTTTAATAAATATAAAAAGAAAGTCGAGCATCGAATCTATCTTAATCCTAGTTTTTGATGAGATATTTTTATTATtgctttagctgct                                                                               >  1:2721240/1‑128 (MQ=255)

                                               aaaaCAACACTCATCTTATGAAAAAAGTTATCTAAAAACTTTTAATAAATATAAAAAGAAAGTCGAGCATCGAATCTATCTTAAAACTAGTTTTTGATGAGATATTTTTATTATtgctttagctgctt                                                                              >  1:1012191/1‑128 (MQ=255)

                                                 aacaacACTCATCTTATGAAAAAAGTTATCTAAAAACTTTTAATAAATATAAAAAGAAAGTCGAGCATCGAATCTATCTTAAAACTAGTTTTTGATGAGATATTTTTATTATtgcttta                                                                                     >  1:1570147/1‑119 (MQ=255)

                                                   caacaCTCATCTTATGAAAAAAGTTATCTAAAAACTTTTAATAAATATAAAAAGAAAGTCGAGCATCGAATCTATCTTAAAACTAGTTTTTGATGAGATATTTTTATTATtgctttagctgctttagc                                                                          <  1:1806090/128‑1 (MQ=255)

                                                       tcTCATCTTATGAAAAAAGTTATCTAAAAACTTTTAATAAATATAAAAAGAAAGTCGAGCATCGAATCTATCTTAAAACTAGTTTTTGATGAGATATTTTTATTATTGCTTTAGCTGCTTTAGCAAAt                                                                      <  1:2669080/127‑1 (MQ=255)

                                                          cATCTTATGAAAAAAGTTATCTAAAAACTTTTAATAAATATAAAAAGAAAGTCGAGCATCGAATCTATCTTAAAACTAGTTTTTGATGAGATATTTTTATTATTGCTTTAGCTGCTTTAGCAAATACt                                                                   <  1:868477/128‑1 (MQ=255)

                                                           aTCTTATGAAAAAAGTTATCTAAAAACTTTTAATAAATATAAAAAGAAAGTCGAGCATCGAATCTATCTTAAAACTAGTTTTTGATGAGATATTTTTATTATTGCTTTAGCTGCTTTAGCAAATACTa                                                                  >  2:490943/1‑128 (MQ=255)

                                                            tCTTATGAAAAAAGTTATCTAAAAACTTTTAATAAATATAAAAAGAAAGTCGAGCATCGAATCTATCTTAAAACTAGTTTTTGATGAGATATTTTTATTATTGCTTTAGCTGCTTTAGCAAATACTAt                                                                 >  1:1141147/1‑128 (MQ=255)

                                                             cTTATGAAAAAAGTTATCTAAAAACTTTTAATAAATATAAAAAGAAAGTCGAGCATCGAATCTATCTTAAAACTAGTTTTTGATGAGATATTTTTATTATTGCTTTAGCTGCTTTAGCAAATACTAtt                                                                <  1:393902/128‑1 (MQ=255)

                                                              ttATGAAAAAAGTTATCTAAAAACTTTTAATAAATATAAAAAGAAAGTCGAGCATCGAATCTATCTTAAAACTAGTTTTTGATGAGATATTTTTATTATTGCTTTAGCTGCTTTAGCAAATACTATTa                                                               >  2:596297/1‑128 (MQ=255)

                                                              ttATGAAAAAAGTTATCTAAAAACTTTTAATAAATATAAAAAGAAAGTCGAGCATCGAATCTATCTTAAAACTAGTTTTTGATGAGATATTTTTATTATTGCTTTAGCTGCTTTAGAAAATACTATTa                                                               >  2:1795804/1‑128 (MQ=255)

                                                               tctGAAAAAAGTTATCTAAAAACTTTTAATAAATATAAAAAGAAAGTCGGGCATCGAATCTATCTTAAAACTAGTTTTTGATGAGATATTTTTATTATTGCTTTAGCTGCTTTAGCAAATACTATTAc                                                              <  2:2165732/126‑1 (MQ=255)

                                                               taagAAAAAAGTTATCTAAAAACTTTTAATAAATATAAAACGAAAGTCGAGCATCGAATCTATCTTAAAACTAGTTTTTGATGAGATATTTTTATTATTGCTTTAGCTGCTTTAGCAAATACTATTAc                                                              <  1:2261376/125‑1 (MQ=255)

                                                                 tGAAAAAAGTTATCTAAAAACTTTTAATAAATATAAAAAGAAAGTCGAGCATCGAATCTATCTTAAAACTAGTTTTTGATGAGATATTTTTATTATTGCTTTAGCTGCTTTAGCAAATACTATTACaa                                                            >  2:643009/1‑128 (MQ=255)

                                                                   aaaaaaGTTATCTAAAAACTTTTAATAAATATAAAAAGAAAGTCGAGCATCGAATCTATCTTAAAACTAGTTTTTGATGAGATATTTTTATTATTGCTTTAGCTGCTTTAGCAAATACTATTACAACt                                                          <  2:250955/128‑1 (MQ=255)

                                                                    aaaaaGTTATCTAAAAACTTTTAATAAATATAAAAAGAAAGTCGAGCATCGAATCTATCTTAAAACTAGTTTTTGATGAGATATTTTTATTATTGCTTTAGCTGCTTTAGCAAATACTATTACAACTg                                                         <  2:2317736/128‑1 (MQ=255)

                                                                    aaaaaGTTATCTAAAAACTTTTAATAAATATAAAAAGAAAGTCGAGCATCGAATCTATCTTAAAACTAGTTTTTGATGAGATATTTTTATTATTGCTTTAGCTGCTTTAGCAAATACTATTACAACTg                                                         <  1:1464109/128‑1 (MQ=255)

                                                                     aaaaGTTATCTAAAAACTTTTAATAAATATAAATAGAAAGTCGAGCATCGAATCTATCTTAAAACTAGTTTTTGATGAGATATTATTATTATTGCTATAGCTGCTTTAGCAAATACTATTACAACTGa                                                        >  2:2692653/1‑128 (MQ=255)

                                                                     aaaaGTTATCTAAAAACTTTTAATAAATATAAAAAGAAAGTCGAGCATCGAATCTATCTTAAAACTAGTTTTTGATGAGATATTTTTATTTTTGCTTTAGCTGCTTTAGCAAATACTATTACAACTGa                                                        <  1:1404887/128‑1 (MQ=255)

                                                                     aaaaGTTATCTAAAAACTTTTAATAAATATAAAAAGAAAGTAGAGCATCGAATCTATCTTAAAACTAGTTTTTGATGAGATATTTTTATTATtgctttagctgc                                                                                <  2:695231/104‑1 (MQ=255)

                                                                      aaaGTTATCTAAAAACTTTTAATAAATATAAAAAGAAAGTCGAGCATCGAATCTATCTTAAAACTAGTTTTTGATGAGATATATTTATTATTGATTTAGCTGCTTTAGCAAATACTATTACAACTGat                                                       >  1:2844204/1‑128 (MQ=255)

                                                                       aaGTTATCTAAAAACTTTTAATAAATATAAAAAGAAAGTCGAGCATCGAATCTATCTTAAAACTAGTTTTTGATGAGATATTTTTATTATTGCTTTAGCTGCTTTAGCAAATACTATTACAACTGatt                                                      >  2:2212504/1‑128 (MQ=255)

                                                                       aaGTTATCTAAAAACTTTTAATAAATATAAAAAGAAAGTCGAGCATCGAATCTATCTTAAAACTAGTTTTTGATGAGATATTTTTATTATTGCTTTAGCTGCTTTAGCAAATACTATTACAAATGatt                                                      >  1:1098649/1‑128 (MQ=255)

                                                                          ttATCTAAAAACTTTTAATAAATATAAAAAGAAAGTCGAGCATCGAATCTATCTTAAAACTAGTTTTTGATGAGATTTTTTTATTATTGCTTTAGCTGCTTTAGCAAATACTATTACAACTGattatt                                                   >  1:2093862/1‑128 (MQ=255)

                                                                            ctCTAAAAACTTTAAATAAATATAAAAAGAAAGGCGAGCATCGAAGCTCTCTTAAAACTAGTTTTTGATGAGATATTTTTATTATTGCTTTAGCTGCTTTAGCAAATACTATTACAACTGAttatttt                                                 <  1:2299500/127‑1 (MQ=255)

                                                                            aTCTAAAAACTTTTAATAAATATAAAAAGAAAGTCTAGCATCGAATCTATCTTAAAACTATTATTTGATGAGATATTTTTATTATTGCTTTAGCTGCTTTAGCAATTACTATTACAACTGCttattta                                                 >  2:1309772/1‑127 (MQ=255)

                                                                             tCTAAAAACTTTTAATAAATATAAAAAGAAAGTCGAGCATCGAATCTATCTTAAAACTAGTTTTTGATGAGATATTTTTATTATTGCTTTAGCTGCTTTAGCAAATACTATTACAACTGAttatttta                                                <  2:1069689/128‑1 (MQ=255)

                                                                              cTAAAAAATTTTAATAAATATAAAAAGACAGTCGAGCATCGATTCTATCTTAAAACTAGTTTTTGATGAGATATTTTTATTATTGCTTTAGCTGCTTTAGCAAATACTATTACAACTGAttattttat                                               <  1:1307874/128‑1 (MQ=255)

                                                                               tAAAAACTTTTAATAAATATAAAAAGAAAGTCGAGCATCGATTCTATCTTAAAACTATTTTTTGATGAGATATTTTTATTTTTGCTTTAGCTGCTTTAGCAAATACTATTACAACTGAtttttgtatt                                              >  1:2900315/1‑128 (MQ=255)

                                                                                 aaaaCTTTTAATAAATATAAAAAGAAAGTCGAGCATCGAATCTATCTTAAAACTAGTTTTTGATGAGATATTTTTATTATTGCTTTAGCTGCTTTAGCAAATACTATTACAACTGATtatttaatttc                                            >  1:2754712/1‑127 (MQ=255)

                                                                                 aaaaCTTTTAATAAATATAAAAAGAAAGTCGAGCATCGAATCTATCTTAAAACTAGTTTTTGATGAGATATTTTTATTATTGCTTTAGCTGCTTTAGCAAATACTATTACAACTGATTattttatttt                                            >  2:1462727/1‑128 (MQ=255)

                                                                                  aaaCTTTTAATAAATATAAAAAGAAAGTCGAGCATCGAATCTATCTTAAAACTAGTTTTTGATGAGATATTTTTATTATTGCTTTAGCTGCTTTAGCAAATACTATTACAACTGATTAttttatttta                                           >  1:1195223/1‑128 (MQ=255)

                                                                                    acatttAATAAATATAAAAAGAAAGGCGAGCATCGAATCTATCTTAAAACTAGTTTTTGATGAGATATTTTTATTATTGCTTTAGCTGCTTTAGCAAATACTATTACAACTGATTATTTTATTTTAGc                                         <  1:1621702/125‑1 (MQ=255)

                                                                                    aCTTTTAATAAATATAAAAAGAAAGTCGAGCATCGAATCTATCTTAAAACTAGTTTTTGATGAGATATTTTTATTATTGCTTTAGCTGCTTTAGCAAATACTATTACAACTGATTATTTTATTTTAGc                                         <  1:2163347/128‑1 (MQ=255)

                                                                                       tttAATAAATATAAAAAGAAAGTCGAGCATCGAATCTATCTTAAAACTAGTTTTTGATGAGATATTTTTATTATTGCTTTAGCTGCTTTAGCAAATACTATTACAACTGATTATTTTATTTTATCAAc                                      >  1:729899/1‑128 (MQ=255)

                                                                                          aataaataTAAAAAGAAAGTCGAGCATCGAATCTATCTTAAAACTAGTTTTTGATGAGATATTTTTATTATTGCTTTAGCTGCTTTAGCaa                                                                        >  1:784893/1‑91 (MQ=255)

                                                                                          aataaataTAAAAAGAAAGTCGAGCATCGAATCTATCTTAAAACTAGTTTTTGATGAGATATTTTTATTATTGCTTTAGCTGCTTTAGCAAATACTATTACAACTGATTATTTTATTTTCGCAACTgg                                   >  1:3001049/1‑128 (MQ=255)

                                                                                             aaataTAAAAAGAAAGTCGAGCATCGAATCTATCTTAAAACTAGTTTTTGATGAGATATTTTTATTATTGCTTTAGCTGCTTTAGCAAATACTATTACACCTGATTATTTTATTTGAGCAACTGgaga                                >  1:618540/1‑128 (MQ=255)

                                                                                             aaataTAAAAAGAAAGTCGAGCATCGAATCTATCTTAAAACTAGTTTTTGATGAGATATTTTTATTATTGCTTTAGCTGCTTTAGCAAATACTATTACAACTGATTATTTTATTTTAGCAACTGgaga                                >  2:375230/1‑128 (MQ=255)

                                                                                             aaataTAAAAAGAAAGTCGAGCATCGAATCTATCTTAAAACTAGTTTTTGATGAGATATTTTTATTATTGCTTTAGCTGCTTTAGCAAATACTATTACAACTGATTATTTTATTTTAGCAACTGgaga                                >  2:902517/1‑128 (MQ=255)

                                                                                              aataTAAAAAGAAAGTCGAGCATCGAATCTATCTTAAAACTAGTTTTTGATGAGATATTTTTATTATTGCTTTAGCTGCTTTAGCAAATACTATTACAACTGATTATTTTATTTTAGCAACTGGAGAt                               <  2:1200586/128‑1 (MQ=255)

                                                                                              aataTAAAAAGAAAGTCGAGCATCGAATCTATCTTAAAACTAGTTTTTGATGAGATATTTTTATTATTGCTTTAGCTGCTTTAGCAAATACTATTACAACTGATTATTTTATTTTAGCAACTGGAGAt                               >  2:332280/1‑128 (MQ=255)

                                                                                              aataTAAAAAGAAAGTCGAGCATCGAATCTATCTTAAAACTAGTTTTTGATGAGATATTTTTATTATTGCTTTAGCTGCTTTAGCAAATACTATTACAACTGATTATTTTATTTTAGCAACTGGAGAt                               >  2:1407511/1‑128 (MQ=255)

                                                                                              aataTAAAAAGAAAGTCGAGCATCGAATCTATCTTAAAACTAGTTTTTGATGAGATATTTTTATTATTGCTTTAGCTGCTTTAGCAAATACTATTACAACAGATTATTTTATTTTAGAAACTGGAGAt                               >  2:166820/1‑128 (MQ=255)

                                                                                               atatAAAAAGAAAGTCGAGCATCGAATCTATCTTAAAACTAGTTTTTGATGAGATATTTTTATTATTGCTTTAGCTGCTTTAGCAAATACTATTACAACTGATTATTTTATTTTAGCAACTGGAGATa                              >  2:417088/1‑128 (MQ=255)

                                                                                               atatAAAAAGAAAGTCGAGCATCGAATCTATCTTAAAACTAGTTTTTGATGAGATATTTTTATTATTGCTTTAGCTGCTTTAGCAAATACTATTACAACTGATTATTTTATTTTAGCAACTGGAGATa                              >  2:800027/1‑128 (MQ=255)

                                                                                               atatAAAAAGAAAGTCGAGCATCGAATCTATCTTAAAACTAGTTTTTGATGAGATATTTTTATTATTGCTTTAGCTGCTTTAGCAAATACTATTACAACTGATTATTTTATTTTAGCAACTGGAGATa                              <  1:2502898/128‑1 (MQ=255)

                                                                                                tataACTAGAAAGTCGAGCATCGAATCTATCTTAAAACTAGTTTTTGATGAGATATTTTTATTATTGCTTTAGCTGCTTTAGCAAATACTATTACAACTGATTATTTTATTTTAGCAACTGGAGATAc                             <  2:1737525/128‑1 (MQ=255)

                                                                                                tataAATAGAAAGTAGACCATAGAATATATCTTATAACTAGTATTTGATGATATATTTTTATTTTTGTTTTAGCTGATTTAGCAAATACTATTACATCTGATTATTTTATTTTAGCAACTGGAGATAc                             <  1:1257815/128‑1 (MQ=255)

                                                                                                  taAAAAGAAAGTCGAGCATCGAATCTATCTTAAAACTAGTTTTTGATGAGATATTTTTATTATTGCTTTAGCTGCTTTAGCAAATACTATTACAACTGATTATTTTATTTTAGCAACTGGAGATACTg                           <  2:2369675/128‑1 (MQ=255)

                                                                                                   aaataGAAAGTCGAGCATCGAATTTATCTTAAAACTATTTTTTGATGAGATATTTTTATTATTGCTTTAGCTGCTTTAGCTAATACTATTACAACTGATTATTTTATTTTAGCAACTGGAGATACTgg                          <  2:2986828/128‑1 (MQ=255)

                                                                                                    aaaaGAAAGTCGCGCATCGAATCTATCTTAAAACTAGTTTTTGATGAGATATTTTTATTATTGCTTTAGCTGCTTTAGCAAATACTATTTCAACTGATTATTTTATTTTAGCAACTGGAGATACTGGa                         >  1:3160903/1‑128 (MQ=255)

                                                                                                    aaaaGAAAGTCGAGCATCGAATCTATCTTAAAACTAGTTTTTGATGAGATATTTTTATTATTGCTTTAGCTGCTTTAGCAAATACTATTACAACTGATTATTTTATTTTAGCAACTGGAGATACTGGa                         >  1:3062683/1‑128 (MQ=255)

                                                                                                          aagTCGAGCATCGAATCTATCTTAAAACTAGTTTTTGATGAGATATTTTTTTTATTGCTTTAGCTGCTTTAGCAAATACTATTACAACTGATTATTTTATTTTAGCAACTGGAGATACTGGATTAttt                   <  2:2040663/128‑1 (MQ=255)

                                                                                                          aagTCGAGCATCGAATCTATCTTAAAACTAGTTTTTGATGAGATATTTTTATTATTGCTTTAGCTGCTTTAGCAAATACTATTACACCTGATTATTTTATTTTAGCAACTGGAGATACTGGATTAttt                   <  2:2838752/128‑1 (MQ=255)

                                                                                                           agTCGAGCATCGAATCTAGCTTAAAACTAGTTTTTGATGAGATATTTTTATTATTGCTTTAGCTGCTTTAGCAAATACTATTACAACTGATTATTTTATTTTAGCAACTGGAGATACTGGATTATTTc                  <  2:191398/128‑1 (MQ=255)

                                                                                                              cGAGCATCGAATCTATCTTAAAACTAGTTTTTGATGAGATATTTTTATTATTGCTTTAGCTGCTTTAGCAAATACTATTACAACTGATTATTTTATTTTAGCAACTGGAGATACTGGATTATTTCCtg               >  2:3105024/1‑128 (MQ=255)

                                                                                                               gAGCATCGAATCTATCTTAAAACTAGTTTTTGATGAGATATTTTTATTATTGCTTTAGCTGCTTTAGCAAATACTATTACAACTGATTATTTTATTTTAGCAACTGGAGATACTg                           <  1:2603595/115‑1 (MQ=255)

                                                                                                                aGCTTCGAATCTATCTTAAATCTAGTTTTTGATGAGATATTTTTATTATTGCTTTAGCTGCTTTAGCAAATACTATTACAACTGATTATTTTATTTTAGCAACTGGAGATACTGGATTATTTCCtggt             <  2:745754/128‑1 (MQ=255)

                                                                                                                aGCATCGAATCTATCTTAAAACTAGTTTTTGATGAGATATTTTTATTATTGCTTTAGCTGCTTTAGCAAATACTATTACAACTGATTATTTTATTTTAGCAACTGGAGATACTAGATTAtttacggtg             >  2:2733304/1‑122 (MQ=255)

                                                                                                                 gCATCGAATCTATCTTAAAACTAGTTTTTGATGAGATATTTTTATTATTGCTTTAGCTGCTTTAGCAAATACTATTACAACTGATTATTTTATTTTAGCAACTGGAGATACTgg                          <  1:530138/114‑1 (MQ=255)

                                                                                                                    tCGAATCTATCTTAAAACTAGTTTTTGATGAGATATTTTTATTATTGCTTTAGCTGCTTTAGCAAATACTATTACAACTGATTATTTTATTTTAGCAACTGGAGATACTGGATTATTTCCTGGTGGTa         >  2:871790/1‑128 (MQ=255)

                                                                                                                    tCGAATCTATCTTAAAACTAGTTTTTGATGAGATATTTTTATTATTGCTTTAGCTGCTTTAGCAAATACTATTACAACTGATTATTTTATTTTAGCAACTGGAGATACTGGATTATTTCCTGGTGGTa         <  2:1520628/128‑1 (MQ=255)

                                                                                                                    tCGAATCTATCTTAAAACTAGTTTTTGATGAGATATTTTTATTATTGCTTTAGCTGCTTTAGCAAATACTATTACAACTGATTATTTTATTTTAGCAACTGGAGATACTGGATTATTTCCTGGTGGTa         >  2:2610318/1‑128 (MQ=255)

                                                                                                                     cGAATCTATCTTAAAACTAGTTTTTGATGAGATATTTTTATTATTGCTTTAGCTGCTTTAGCAAATACTATTACAACTGATTATTTTATTTTAGCTACTGGAGATACTGGATTATTTACTGGTGGTAc        >  2:303728/1‑128 (MQ=255)

                                                                                                                     cGAATCTATCTTAAAACTAGTTTTTGATGAGATATTTTTATTATTGCTTTAGCTGCTTTAGCAAATACTATTACAACTGATTATTTTATTTTAGCAACTGGAGATACTTGATTATTTCCTGGTGGTAc        >  1:2443441/1‑128 (MQ=255)

                                                                                                                         tCTATCTTAAAACTAGTTTTTGATGAGATATTTTTATTATTGCTTTAGCTGCTTTAGCAAATACTATTACAACTGATTATTTTATTTTAGCAACTGGAGATACTGGATTATTTCCTGGTGGTACTGCt    <  2:2093862/128‑1 (MQ=255)

                                                                                                                         tCTATATTAAAAATATTTTTTGATTAGATATTTTTATTATTGCTTTAGCTGCTTTAGCAAATACTATTACAACTGATTATTTTATTTTAGCAACTGGAGATACTGGATTATTTCCTGGTGGTACTGCt    <  1:67742/128‑1 (MQ=255)

AAACCTAAAAAAGCCCCCCCATTAACTATTGAAGAAATTAATGAAATAAAACAACACTCATCTTATGAAAAAAGTTATCTAAAAACTTTTAATAAATATAAAAAGAAAGTCGAGCATCGAATCTATTTTAAAACTAGTTTTTGATGAGATATTTTTATTATTGCTTTAGCTGCTTTAGCAAATACTATTACAACTGATTATTTTATTTTAGCAACTGGAGATACTGGATTATTTCCTGGTGGTACTGCTAC  >  CP016816/257969‑258219

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`