BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP016816	32,707	A→T	I1067F (ATT→TTT)	JCVSYN2_00105 →	ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP016816	32,707	0	A	T	99.4%	4263.4 / NA	1356	I1067F (ATT→TTT)	JCVSYN2_00105	ABC transporter permease
Reads supporting (aligned to +/- strand): ref base A (3/5); new base T (694/650); total (699/657)
Fisher's exact test for biased strand distribution p-value = 4.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1403 total aligned reads displayed.

TTACTCAACCTTTAACTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTATTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACCTAAATCAAATAACATTGA  >  CP016816/32582‑32831

tttcTCAACCTTTAACTCAGTTTGTTGGAGATCTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCAtttttt                                                                                                                            <  2:656468/125‑1 (MQ=255)

 tACTCAACCTTTAACTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGATAAGAAAAGATCTTAAACTTATTTTTAGGTTATTCTCCTTCATttttta                                                                                                                           >  1:2504311/1‑128 (MQ=255)

 tACTCAACCTTTAACTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATttttta                                                                                                                           <  2:2158034/128‑1 (MQ=255)

  aCTCAACCTTTAACTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTaa                                                                                                                          >  2:2368798/1‑128 (MQ=255)

  aCTCAACCTTTAACTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTaa                                                                                                                          <  1:3044368/128‑1 (MQ=255)

  aCTCAACCTTTAACTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTaa                                                                                                                          <  2:1756923/128‑1 (MQ=255)

  aCTCAACCTTTAACTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCGTAAACTTAATTTTAGGGTATTCTCCTTCATTTTTTaa                                                                                                                          >  2:1819164/1‑128 (MQ=255)

    tCAACCTTTAACTCAGTTTGTTGGAGATCTTTTTAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCTTTTTTTAAAt                                                                                                                        <  2:706136/128‑1 (MQ=255)

      aaCCTTTAACTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCt                                                                                                                      <  2:1057036/128‑1 (MQ=255)

       aCCTTTAACTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAAt                                                                                                                        >  2:2462192/1‑125 (MQ=255)

       aCCTTTAACTCAGTTTGTTGGAGAACTTTTAAAAAATATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCtt                                                                                                                     >  2:1403513/1‑128 (MQ=255)

        ccTTTAACTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTa                                                                                                                    <  1:2215017/128‑1 (MQ=255)

        ccTTTAACTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTa                                                                                                                    <  2:2907562/128‑1 (MQ=255)

         cTTTAACTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTAt                                                                                                                   >  1:1617181/1‑128 (MQ=255)

          tttAACTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTAtt                                                                                                                  >  1:1166764/1‑128 (MQ=255)

          tttAACTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTAAAAGATAAAGTGCAAACTAAAGGTGAGTGAAATGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTAtt                                                                                                                  >  1:175602/1‑128 (MQ=255)

           ttAACTCATTTTGTTGGAGAACTTTTAAAAAGTATTTTTAAAGATAAAGTGCAAACTAAAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGTTTATTCTCCTTCATTTTTTAAATCTTAttt                                                                                                                 <  1:2331204/128‑1 (MQ=255)

             aaCTCAGTTTGTTGTAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTaa                                                                                                               <  1:3098634/128‑1 (MQ=255)

              aCTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAAc                                                                                                              >  1:258724/1‑128 (MQ=255)

              aCTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAAc                                                                                                              <  2:286005/128‑1 (MQ=255)

              aCTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAAc                                                                                                              <  2:1217322/128‑1 (MQ=255)

                  aGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGt                                                                                                          >  1:698707/1‑128 (MQ=255)

                    tttGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGa                                                                                                        >  1:1105641/1‑128 (MQ=255)

                    tttGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATATTATTTAACAAGTGa                                                                                                        <  2:2961638/128‑1 (MQ=255)

                       gttgGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAAtc                                                                                                     >  1:461841/1‑128 (MQ=255)

                        ttgGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAACCTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAAtct                                                                                                    <  1:2043138/128‑1 (MQ=255)

                           gagaACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGa                                                                                                 <  2:1304025/128‑1 (MQ=255)

                            agaACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGaa                                                                                                >  1:2268844/1‑128 (MQ=255)

                            agaACTTTTAAAAACTATTTATAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCCTTTTTTAAATCTTATTTAACAAGTGAATCTCGaa                                                                                                <  2:2036549/128‑1 (MQ=255)

                             gaACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAag                                                                                               >  2:2807526/1‑128 (MQ=255)

                             gaACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAag                                                                                               >  1:422373/1‑128 (MQ=255)

                              aaCTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAaga                                                                                              >  1:50495/1‑128 (MQ=255)

                               aCTTTTAATTACTTTTTTTAAAGATAAAGTGCATACTACTGGTGAGTGAAAAGAATAGATCTTAAAGTTAATTTTAGGTTATTCTCCTTCATTTTTTTAATCTTATTTAACAAGTGTATCTCGAagag                                                                                             <  2:1658972/128‑1 (MQ=255)

                                cTTTTATACACTATTTTTATAGATAATGTGCCAACTACAGGTGAGGGAAAAGAATAGATCTTAACCTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGc                                                                                            <  2:3103051/128‑1 (MQ=255)

                                 ttttAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCt                                                                                           <  2:2246346/128‑1 (MQ=255)

                                  tttAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGGTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTc                                                                                          <  2:348910/128‑1 (MQ=255)

                                    tAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCaa                                                                                        >  2:874798/1‑128 (MQ=255)

                                         aatATTTTTAAAGATAATGTTCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTc                                                                                   <  1:628105/126‑1 (MQ=255)

                                         aCTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGCTTCTCGCAGAGCTCCAGTTTc                                                                                   >  1:2174631/1‑128 (MQ=255)

                                         aCTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTc                                                                                   >  1:893076/1‑128 (MQ=255)

                                          cTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCt                                                                                  >  1:1814589/1‑128 (MQ=255)

                                           tATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCTAAGAGCTCAATTATCtt                                                                                 >  2:413279/1‑128 (MQ=255)

                                             ttattAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCtttt                                                                               >  2:924746/4‑128 (MQ=255)

                                               tttAAAGATAAAGTGCTAACTACAGGTGAGTGAAAAGAAAAGATCTAAAACTTAATTTTAGGTAATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTgg                                                                             <  1:1395953/128‑1 (MQ=255)

                                               tttAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTgg                                                                             >  1:209625/1‑128 (MQ=255)

                                               tttAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTgg                                                                             >  2:2319245/1‑128 (MQ=255)

                                                   aaGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGa                                                                         >  1:1558523/1‑128 (MQ=255)

                                                      aTAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACaa                                                                      >  1:2767459/1‑128 (MQ=255)

                                                       tAAAGCGCAAACTACAGGTGAGAGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGCGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACaaa                                                                     <  1:567544/128‑1 (MQ=255)

                                                        aaaGTGCAAACTACAGGTGAGTGAAAAGAAAAGTTCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACaaaa                                                                    <  2:1978507/128‑1 (MQ=255)

                                                        aaaGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACaaaa                                                                    >  1:2935478/1‑128 (MQ=255)

                                                         aaGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTg                                                                              <  2:1939164/117‑1 (MQ=255)

                                                          aGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAaaac                                                                  <  2:529164/128‑1 (MQ=255)

                                                          aGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAaaac                                                                  <  1:1916572/128‑1 (MQ=255)

                                                            tGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAaaacaa                                                                <  2:2535538/128‑1 (MQ=255)

                                                              cAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAaaac                                                                  >  1:953271/1‑124 (MQ=255)

                                                               aatcTACAGGTGAGTGAAAAGATAAGAGCTTAAACTTAATTATAGGTTAATCTCCCTCATTTTTTAAATCTTATTTAACAAGTGAATCACGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACt                                                             <  2:1884594/125‑1 (MQ=255)

                                                               aaaCTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATCTTCTTTTGGTTGACAAAAACAAACt                                                             >  2:1697293/1‑128 (MQ=255)

                                                               aaaCTACAGGTGAGGGAAAAGAACAGATCTTAAACTTAATTTTAGGTTATTCTCCATCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACt                                                             <  1:1330031/128‑1 (MQ=255)

                                                                aaCTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCtttt                                                                               <  2:452257/109‑1 (MQ=255)

                                                                aaCTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTgg                                                                             >  2:257752/1‑111 (MQ=255)

                                                                   tACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTATCAAGTGAATCTCGATGAGCTCAATTTTCTTTTGGTTGAC‑AATACAAACTATAAt                                                        >  2:1441115/1‑128 (MQ=255)

                                                                    aCAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTAAAAt                                                        >  2:1584613/1‑128 (MQ=255)

                                                                         tgagtgaAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACaa                                                                                                            >  2:1372616/1‑71 (MQ=255)

                                                                          gagtgaAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTATATCTTATTTAACAAGTGAATCTCGAAGAGCTCATTTTTCTTTTGGTTTCCAAAAACATACAAAAATTCcaca                                                  >  1:2446346/1‑128 (MQ=255)

                                                                           agtgaAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACaa                                                 <  1:2394836/128‑1 (MQ=255)

                                                                            gtgaAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACaaa                                                >  1:1878931/1‑128 (MQ=255)

                                                                             tgaAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACaaaa                                               <  2:1261688/128‑1 (MQ=255)

                                                                               aaaagaaaagATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAg                                             >  1:2542674/1‑128 (MQ=255)

                                                                                  agaaaagaTCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATc                                          >  1:1023088/1‑128 (MQ=255)

                                                                                    aaaataTCTTAAACTTAATTTTAGGTTATTCTCCATCAATTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCaa                                        <  1:341032/128‑1 (MQ=255)

                                                                                    aaaagaTCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCaa                                        <  1:1967907/128‑1 (MQ=255)

                                                                                      aagaTCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCca                                                    <  2:2616993/114‑1 (MQ=255)

                                                                                      aagaTCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAAtt                                      >  1:2690895/1‑128 (MQ=255)

                                                                                        gaTCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAg                                    <  2:1524662/128‑1 (MQ=255)

                                                                                        gaTCTTAAACTTAATTTTAGGTTATTCTCCTTCAATTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATATTCTATTGGTTGACAAAAACAAACTATAATTCCACAACAAGACCACTTAg                                    >  2:2657943/1‑128 (MQ=255)

                                                                                          tCTTAAAGTTTATTTTAGGTTATTCTCCTTCATTTTTTAAATC‑TATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCaa                                 <  1:2880970/128‑1 (MQ=255)

                                                                                           cTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCaa                                 >  2:585411/1‑128 (MQ=255)

                                                                                            ttAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAAc                                <  1:1718443/128‑1 (MQ=255)

                                                                                            ttAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAAc                                >  2:489407/1‑128 (MQ=255)

                                                                                             tAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAAcg                               >  2:3154164/1‑127 (MQ=255)

                                                                                             tAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACt                               >  2:3024040/1‑128 (MQ=255)

                                                                                             tAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACt                               <  2:220347/128‑1 (MQ=255)

                                                                                              aaaCTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGAAACTa                              >  2:1009889/1‑128 (MQ=255)

                                                                                               aaCTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACATAAAGATCAATTAGCTACTAt                             >  1:762108/1‑128 (MQ=255)

                                                                                               aaCTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTAt                             >  1:825802/1‑128 (MQ=255)

                                                                                                 cTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTAttt                           >  2:209464/1‑128 (MQ=255)

                                                                                                   tAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTAttttt                         <  2:3194417/128‑1 (MQ=255)

                                                                                                   tAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTAttttt                         <  2:1124192/128‑1 (MQ=255)

                                                                                                    aaTTTTAGGTTATTCTCCTTCATTATTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTa                        >  1:1469537/1‑128 (MQ=255)

                                                                                                       tttAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAAc                     <  1:1267101/128‑1 (MQ=255)

                                                                                                        ttAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAAcc                    >  1:1209390/1‑128 (MQ=255)

                                                                                                          aGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGCTCAATTAGCAACTATTTTTAAACCTa                  >  1:2823133/1‑128 (MQ=255)

                                                                                                               aTTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCTCAAAAAGATCAATTAGCAACTATTATTAAACCTAACTCa             >  1:2640647/1‑128 (MQ=255)

                                                                                                                ttCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACCTAAATCaa            >  2:817456/1‑128 (MQ=255)

                                                                                                                   tcCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACCTAAATCAAATa         <  1:3158105/128‑1 (MQ=255)

                                                                                                                     cTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACCTAAATCAAATAAc       <  2:2707175/128‑1 (MQ=255)

                                                                                                                         aTTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACACACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAACCCTACTTCAAATTACATTg   >  2:2761001/1‑128 (MQ=255)

                                                                                                                          ttttttAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACATAAATCAAATAACATTGa  >  2:762560/1‑128 (MQ=255)

                                                                                                                          ttttttAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACATAAAGAAAATAAAAttaa  >  2:1159943/1‑126 (MQ=255)

TTACTCAACCTTTAACTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTATTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACCTAAATCAAATAACATTGA  >  CP016816/32582‑32831

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`