Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 478,738 C→A G301V (GGT→GTT)  JCVSYN2_02210 ← DNA‑directed RNA polymerase subunit beta'

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816478,7380CA99.2% 3504.9 / NA 1212G301V (GGT→GTT) JCVSYN2_02210DNA‑directed RNA polymerase subunit beta'
Reads supporting (aligned to +/- strand):  ref base C (4/5);  new base A (633/562);  total (640/572)
Fisher's exact test for biased strand distribution p-value = 7.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1243 total aligned reads displayed.

GTAACATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTACCACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTCTAAACGTTTTAATAAT  >  CP016816/478613‑478863
                                                                                                                             |                                                                                                                             
 ttaCATATGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTaacac                                                                                                                            <  2:486298/126‑1 (MQ=255)
 tAACATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTaacac                                                                                                                            >  2:543462/1‑128 (MQ=255)
  aaCATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTaacacc                                                                                                                           >  2:2962203/1‑128 (MQ=255)
   aCATACTTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAacacca                                                                                                                          <  1:533607/128‑1 (MQ=255)
   aCATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAacacca                                                                                                                          <  1:742321/128‑1 (MQ=255)
      tACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCt                                                                                                                       >  1:439602/1‑128 (MQ=255)
           ttttCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTg                                                                                                                  <  1:825235/128‑1 (MQ=255)
           ttttCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACAATCTAATTg                                                                                                                  >  2:657347/1‑128 (MQ=255)
            tttCATTATTTACAATTATTGATGGTGCACCCATTTCTTTAACATTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACAATCTAAttta                                                                                                                 >  1:1637773/1‑126 (MQ=255)
               cATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGaata                                                                                                              >  2:2864042/1‑128 (MQ=255)
               cATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGaata                                                                                                              <  1:1580366/128‑1 (MQ=255)
                attattTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGaataa                                                                                                             <  2:2563328/128‑1 (MQ=255)
                attattTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGaataa                                                                                                             <  1:35750/128‑1 (MQ=255)
                  tattTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAAtt                                                                                                           <  1:1118063/128‑1 (MQ=255)
                  tattTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAAtt                                                                                                           <  2:2006883/128‑1 (MQ=255)
                  tattTAAAATAATTGATGGAGCACACATTTCATTAAATTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAAtt                                                                                                           <  1:1260467/128‑1 (MQ=255)
                   attTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTg                                                                                                          >  2:1726338/1‑128 (MQ=255)
                    tttACTATATTTGATGGTGCAACTATTTGTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTgg                                                                                                         <  1:2081813/128‑1 (MQ=255)
                      tACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACCCCATATAATTTAATAATAGGAc                                                                                                       >  2:2111879/1‑128 (MQ=255)
                       aCAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACg                                                                                                      >  2:1918734/1‑128 (MQ=255)
                       aCAATAATTGATGGTGCACCCATTTCATTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACg                                                                                                      <  2:606696/128‑1 (MQ=255)
                         aataatTGATGGTGCACCCATTTCTTTAACTTTTTTTAATGTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGTACTaa                                                                                                    >  1:1791355/1‑128 (MQ=255)
                           taatTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTATTTGAATAAGTGGACGAATg                                                                                                  >  2:1880852/1‑128 (MQ=255)
                           taatTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATg                                                                                                  >  2:1035674/1‑128 (MQ=255)
                            aatTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGt                                                                                                 <  1:298473/128‑1 (MQ=255)
                             attgattgTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTc                                                                                                <  1:2489567/128‑1 (MQ=255)
                                 aTGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAAtt                                                                                                           <  2:2206616/113‑1 (MQ=255)
                                  tGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCtggtg                                                                                           >  2:1125775/1‑128 (MQ=255)
                                   ggTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTATCACCATCTAATTGAATAATTGGACGATTGTCaggtgg                                                                                          >  2:2686432/1‑128 (MQ=255)
                                    gTGAACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGa                                                                                         <  1:707344/128‑1 (MQ=255)
                                     ttactatctttCTTTAACTTTTTTTAATATTTCATTTCTAATAATTATTATTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGaa                                                                                        <  1:1831446/120‑1 (MQ=255)
                                      gCACCAATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAAt                                                                                       <  1:869174/128‑1 (MQ=255)
                                       cACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGATTAATTGGACGATTGTCTGGTGGAATa                                                                                      >  1:392009/1‑128 (MQ=255)
                                       cACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATa                                                                                      >  1:2053714/1‑128 (MQ=255)
                                        aCCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATaa                                                                                     >  2:93645/1‑128 (MQ=255)
                                        aCCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATaa                                                                                     <  2:1454568/128‑1 (MQ=255)
                                         cccATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGTACGAATGTCTGGTGTAATAAc                                                                                    >  1:2679219/1‑128 (MQ=255)
                                         cccATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAAc                                                                                    <  1:2429831/128‑1 (MQ=255)
                                             tttCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGa                                                                                >  2:1590577/1‑128 (MQ=255)
                                              ttCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGaa                                                                               >  2:1501212/1‑128 (MQ=255)
                                               tcatttaCTTTTTTTAATCTTTAATTTCTAATAATGATTCTTCTATATATATCATTAATTTCAGAAGTAGTAATTCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAAt                                                                              <  2:775338/122‑1 (MQ=255)
                                                cTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAAtt                                                                             <  1:2629277/128‑1 (MQ=255)
                                                 tttAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTa                                                                            <  2:1381580/128‑1 (MQ=255)
                                                  ttAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTAc                                                                           >  2:3143304/1‑128 (MQ=255)
                                                   tAACTTATTTTATTCTTTGATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTATCACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTAct                                                                          <  1:2251225/128‑1 (MQ=255)
                                                    aaCTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATAGAATAATTGGACGAATGTCTGTTGGAATAACTGGAAGTActc                                                                         >  2:2414610/1‑128 (MQ=255)
                                                     tatttttttAATCTTTCATTTATAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTActct                                                                        <  1:2816140/126‑1 (MQ=255)
                                                     aCTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGAAGAATGTCTGGTGGAATAACTGGAATTActct                                                                        <  1:2464430/128‑1 (MQ=255)
                                                      cTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACtctc                                                                       >  1:1520672/1‑128 (MQ=255)
                                                        ttttttAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCAt                                                                     >  1:3187029/1‑128 (MQ=255)
                                                        ttttttAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGAAAATCTAACACAATCTAATTTAATAATTGGACTAATGTCTGGTGTAATAACTGGAATTACTCTCAt                                                                     >  2:2620269/1‑128 (MQ=255)
                                                         tttttAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATa                                                                    >  1:259580/1‑128 (MQ=255)
                                                           tttAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAAc                                                                  <  2:2764856/128‑1 (MQ=255)
                                                             tAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACca                                                                <  1:585500/128‑1 (MQ=255)
                                                                tCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACcattc                                                             >  1:14006/1‑128 (MQ=255)
                                                                 cTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCACAAGTAGTAAATCTAACACCATCTAATTGAATAATTGTACGAATTTCTCGAGGAATAACTGGATTTACTATCATAAGcattaa                                                            >  1:1672804/1‑126 (MQ=255)
                                                                  tttCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGATTAATTGGACGTATGTCTGGTGGAATTACTGGAATTACTATCATATCcatgcat                                                           >  1:1887365/1‑128 (MQ=255)
                                                                  tttCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACcattcat                                                           >  1:1963560/1‑128 (MQ=255)
                                                                  tttAATTTCTAATAATGATTCTTCTATATAAATCATTTATTTCAGAAGTATTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACcattcat                                                           <  1:2937833/128‑1 (MQ=255)
                                                                   ttCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACcatt                                                              >  2:410416/1‑124 (MQ=255)
                                                                     cATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCAttcattca                                                        >  2:3081992/1‑128 (MQ=255)
                                                                     cATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCAttcattca                                                        >  2:2911897/1‑128 (MQ=255)
                                                                      aTTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAg                                                       <  2:1237890/128‑1 (MQ=255)
                                                                       tttCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAgg                                                      <  1:1147511/128‑1 (MQ=255)
                                                                        ttCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTCGAATAACTGGAATTACTCTCATAACCATTCATTCAGGa                                                     >  1:30494/1‑128 (MQ=255)
                                                                         tCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATACCCATTCATTCAGGAc                                                    >  1:288157/1‑128 (MQ=255)
                                                                         tCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGAc                                                    >  2:118231/1‑128 (MQ=255)
                                                                          cTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACg                                                   <  2:71788/128‑1 (MQ=255)
                                                                           taataaTTATTAATCTATATAAATAATTTATTTCAGAATTAGGAAATCTTACCCCCTATAATTGAATAATTGGACGAATGTCTGGTTGTATTACTGGAATTAATGGAATAACCATTAATTCCGGACGt                                                  >  1:100031/1‑128 (MQ=255)
                                                                            aataatGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGtt                                                 >  1:927483/1‑128 (MQ=255)
                                                                             ataatGATTCTTCTATATAAATCATTAATTGCAGAAGGAGTAAATCAAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTg                                                <  1:1473507/128‑1 (MQ=255)
                                                                               aatGATTCGTCTATATAAATCAATAATTTCAGCAGTACTAAATCTAACACCAGCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGt                                              <  1:2378562/128‑1 (MQ=255)
                                                                                attaTTCTTCAATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGtt                                             <  1:1745910/125‑1 (MQ=255)
                                                                                atGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCATGACGTTGGtt                                             >  2:1331211/1‑128 (MQ=255)
                                                                                atGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGtt                                             >  1:3155062/1‑128 (MQ=255)
                                                                                    ttcttcTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGaa                                         <  2:2315208/128‑1 (MQ=255)
                                                                                    ttcttcTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGaa                                         >  1:2740822/1‑128 (MQ=255)
                                                                                     tcttctATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAAc                                        >  2:3020933/1‑128 (MQ=255)
                                                                                      cttctATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAAATGAATAATTTGACGAAAGTCTGGTGGAATAACAGGAATTACTCTCATAACCATTAATTCAGGACGTTGGTTAGacag                                       >  1:1431079/1‑125 (MQ=255)
                                                                                        tctATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTa                                            >  2:2507041/1‑121 (MQ=255)
                                                                                           atataAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTa                                  >  2:3184482/1‑128 (MQ=255)
                                                                                            tataACTCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTaa                                 <  2:2063787/128‑1 (MQ=255)
                                                                                            tataAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTaa                                 <  1:2191056/128‑1 (MQ=255)
                                                                                            tataAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAAATGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTaa                                 <  1:984309/128‑1 (MQ=255)
                                                                                                aaTCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCATGACGTTGGTTAGAACGTTTTAAAGCa                             >  2:2345227/1‑128 (MQ=255)
                                                                                                aaTCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCa                             >  1:4862/1‑128 (MQ=255)
                                                                                                 aTCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCAt                            >  1:3110403/1‑128 (MQ=255)
                                                                                                     ttAATTTCAGAAGTAGTAAAACTAACACCATCTAATTGAATAATTGGACTAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATAGATTCAGGACGTAGGTTAGAACGTTTTAAAtcatcaca                        >  1:1327647/1‑126 (MQ=255)
                                                                                                      tAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCaaaa                       <  1:11974/128‑1 (MQ=255)
                                                                                                      tAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCaaaa                       <  2:1828470/128‑1 (MQ=255)
                                                                                                       aaTTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAg                      <  2:32112/128‑1 (MQ=255)
                                                                                                         tttCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGTAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGtt                    <  1:2314727/128‑1 (MQ=255)
                                                                                                          ttCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCAGGTGGAATAACTGGAATTACTCTCATAACCATTCAATCAGGACGTTGGATAGAACGTTGTAAAGCATCAAAAGatt                   >  2:1283336/1‑128 (MQ=255)
                                                                                                             aGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTcct                >  2:81834/1‑126 (MQ=255)
                                                                                                                 gtagtaAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGGGGATTAACTGGAATTACTCTCATATCCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTCTAAACg            <  2:1668315/128‑1 (MQ=255)
                                                                                                                  tagtaAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTCTAAACGt           >  2:24677/1‑128 (MQ=255)
                                                                                                                  tagtaAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTGTCTAAAGGt           >  1:632514/1‑128 (MQ=255)
                                                                                                                       aaTCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTATTAAAGCATCAAAAGTTTCTAAACGTTTtaa      >  2:4403/1‑128 (MQ=255)
                                                                                                                        aTCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTCTAAACGTTTtaat     >  1:105242/1‑128 (MQ=255)
                                                                                                                        aTCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTCTAAACGATTgaat     >  2:2171017/1‑128 (MQ=255)
                                                                                                                             |                                                                                                                             
GTAACATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTACCACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTCTAAACGTTTTAATAAT  >  CP016816/478613‑478863

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑