BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP016816	420,163	2 bp→TT	intergenic (‑446/+396)	JCVSYN2_01895 ← / ← JCVSYN2_01900	30S ribosomal protein S10/tRNA‑Thr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP016816	420,163	0	A	T	99.5%	12815.3 / NA	4124	intergenic (‑446/+397)	JCVSYN2_01895/JCVSYN2_01900	30S ribosomal protein S10/tRNA‑Thr
Reads supporting (aligned to +/- strand): ref base A (15/5); new base T (2212/1877); total (2230/1894)
Fisher's exact test for biased strand distribution p-value = 7.26e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	CP016816	420,164	0	A	T	98.7%	11131.2 / NA	4083	intergenic (‑447/+396)	JCVSYN2_01895/JCVSYN2_01900	30S ribosomal protein S10/tRNA‑Thr
Reads supporting (aligned to +/- strand): ref base A (9/44); new base T (2200/1828); total (2210/1873)
Fisher's exact test for biased strand distribution p-value = 3.12e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 4134 total aligned reads displayed.

ATTCATTCATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTAAAATTAAAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑TTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTAAACGTATATTTATG  >  CP016816/420038‑420289

||

   cattcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATa                                                                                                                             <  2:6027968/128‑1 (MQ=255)

    attcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTtaataa                                                                                                                            <  2:730214/128‑1 (MQ=255)

      tcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACt                                                                                                                          >  1:10190987/1‑128 (MQ=255)

      tcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACt                                                                                                                          <  1:9357459/128‑1 (MQ=255)

      tcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACt                                                                                                                          <  1:4233881/128‑1 (MQ=255)

         tACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGaa                                                                                                                       <  2:9807060/128‑1 (MQ=255)

         tACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGaa                                                                                                                       <  2:2077406/128‑1 (MQ=255)

          aCTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAat                                                                                                                      >  2:479511/1‑128 (MQ=255)

          aCTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAat                                                                                                                      >  2:9381256/1‑128 (MQ=255)

          aCTTTTAGTCTCATAAATCTAAGTATCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAat                                                                                                                      <  2:7721793/128‑1 (MQ=255)

            atttAGTCTAATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATAATTTTATTAGTTGATATATTAGAAAACTACTATCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAatta                                                                                                                    <  2:6588831/127‑1 (MQ=255)

             tttAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAattat                                                                                                                   >  1:77230/1‑128 (MQ=255)

             tttAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAattat                                                                                                                   >  1:3788318/1‑128 (MQ=255)

               tAGTCCCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTAttt                                                                                                                 <  2:3678523/128‑1 (MQ=255)

                    tcatcaATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCaa                                                                                                            <  1:6751348/128‑1 (MQ=255)

                    tcATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCaa                                                                                                            >  2:8896589/1‑128 (MQ=255)

                      aTAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTTAATTATATTTCAAAt                                                                                                          >  2:10415143/1‑128 (MQ=255)

                      aTAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAAt                                                                                                          <  2:8520567/128‑1 (MQ=255)

                      aTAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAAt                                                                                                          >  1:10371145/1‑128 (MQ=255)

                      aTAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAAt                                                                                                          >  1:9207705/1‑128 (MQ=255)

                          aTCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTATAAAAAATTTTTAATTATAATAACTGAATTATATTTCAAATTGtt                                                                                                      >  2:5847207/1‑128 (MQ=255)

                           tCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTa                                                                                                     >  1:500209/1‑128 (MQ=255)

                              aaGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAat                                                                                                  <  1:1966284/128‑1 (MQ=255)

                              aaGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTAATAGCACTTTTATAAAAAATTTTTAATTTTAATAAATGAATTATTATTCAAATTATTAAat                                                                                                  >  2:1504042/1‑128 (MQ=255)

                               aGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAatt                                                                                                 <  2:6399036/128‑1 (MQ=255)

                                  ttCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAattatt                                                                                              >  2:10222467/1‑128 (MQ=255)

                                  ttCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAattatt                                                                                              >  1:1227572/1‑128 (MQ=255)

                                  ttCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAattatt                                                                                              >  2:5640557/1‑128 (MQ=255)

                                    cTAAAATTATTTTATCATAAAAAAAAATTTTACTTTTATTAGTAGATATATTATAAAACTACTAGAACTTTTTTAAAAAATATTAAATTTTAATAACTTAATTATTTTACAAATTCTAAAATAAttta                                                                                            >  2:4245022/1‑127 (MQ=255)

                                     tAAAATTATTTTATCATTTAAAATAATAATAATTTTATTATTTTTTATATTATAAAAATACTATCAATTTTTTAAAAAATTTTTAATTTTAATAACTTAATTATTTTTCAAATTGTTAAATTATTTTc                                                                                           <  1:6315802/128‑1 (MQ=255)

                                      aaaaTTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCa                                                                                          >  2:6195797/1‑128 (MQ=255)

                                         attattTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAAt                                                                                       <  2:5193087/128‑1 (MQ=255)

                                         attattTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAAt                                                                                       <  1:3036125/128‑1 (MQ=255)

                                              tttATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTa                                                                                  >  1:3355356/1‑128 (MQ=255)

                                              tttATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTa                                                                                  >  1:8545706/1‑128 (MQ=255)

                                              tttATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTa                                                                                  >  1:9520988/1‑128 (MQ=255)

                                              tttATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGAACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTa                                                                                  <  1:3193918/128‑1 (MQ=255)

                                               ttATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTAt                                                                                 <  1:1077299/128‑1 (MQ=255)

                                                tATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATc                                                                                <  2:7522426/128‑1 (MQ=255)

                                                tATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATc                                                                                >  1:4459541/1‑128 (MQ=255)

                                                tATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATc                                                                                >  1:5305283/1‑128 (MQ=255)

                                                tATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAAATACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATc                                                                                <  2:9517140/128‑1 (MQ=255)

                                                 aTCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCt                                                                               <  2:494377/128‑1 (MQ=255)

                                                 aTCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCt                                                                               >  1:4036933/1‑128 (MQ=255)

                                                 aTCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCt                                                                               <  1:1718338/128‑1 (MQ=255)

                                                  tCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTa                                                                              >  1:9013695/1‑128 (MQ=255)

                                                  tCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTa                                                                              <  2:710671/128‑1 (MQ=255)

                                                   cATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTAt                                                                             >  2:1117410/1‑128 (MQ=255)

                                                    aTTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTAtt                                                                            <  2:1701187/128‑1 (MQ=255)

                                                        aaaaaaaTAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATc                                                                        >  2:348948/1‑128 (MQ=255)

                                                        aaaaaaaTAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATCTTCAAATCATTATCTATTAATc                                                                        <  1:2574082/128‑1 (MQ=255)

                                                         aaaaaaTAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCa                                                                       <  1:7142305/128‑1 (MQ=255)

                                                            aaaTAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAAtt                                                                    <  1:5728277/128‑1 (MQ=255)

                                                            aaaTAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAAtt                                                                    <  1:6915547/128‑1 (MQ=255)

                                                             aataatACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAAttt                                                                   <  2:6763967/128‑1 (MQ=255)

                                                             aataatACTTTTATTAGTTGATATATTAGAAAACTACTAGCAATTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAAttt                                                                   <  2:2703968/128‑1 (MQ=255)

                                                               taataCTTTTATTAGTTGATATATTAGTAAACTACTAGAACTTTTTTAAAAAATTTTTAATTTTAATAAATGAATTATTTTACAAATTTTTAAATTATTATCTAATAATTATATATTAATCAATTTTa                                                                 >  2:2241858/1‑128 (MQ=255)

                                                                  ttcTTTTATTAGTTTTTATATTAGAAAACTATTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGATTTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGc                                                              <  2:283223/126‑1 (MQ=255)

                                                                    cTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAatta                                                                                                >  2:8737693/1‑92 (MQ=255)

                                                                       ttattaGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATATATTAATCAATATTATGATcata                                                         >  2:8030209/1‑125 (MQ=255)

                                                                        tattaGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATATTTCAAATAGTTAAATTATTTTCAAATCATTATATATTAATCAATTTTATTCTCt‑‑ttt                                                      >  2:6921980/1‑128 (MQ=255)

                                                                         attaGTTTTTAACTTAGTAAACTAATATAACTTTTTTAAAAAATTTTTAATTTTAATAAATTAATTATTTTTTAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑tttt                                                     <  2:9221609/128‑1 (MQ=255)

                                                                           taGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATTCTCG‑‑tttttt                                                   >  2:5465814/1‑128 (MQ=255)

                                                                            atttGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCGCG‑‑ttttttt                                                  <  2:129284/126‑1 (MQ=255)

                                                                                gATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑ttttttttttt                                              >  2:833871/1‑128 (MQ=255)

                                                                                 atatatTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTAG‑‑tttttttttttt                                             >  1:10002761/1‑128 (MQ=255)

                                                                                   atatTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAATTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCT‑‑tttttttttttttt                                           >  2:7874840/1‑128 (MQ=255)

                                                                                   atatTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑tttttttttttttt                                           >  1:2278776/1‑128 (MQ=255)

                                                                                   atatTAGAAAAATAATATCAATTTTTTAAAAAATTTTTAATTTTAATAACTTAATTATTTTTCAAATTTTTAAATTATTTTAAAATCATAATAAATTAATCAATTTTATGCTCG‑‑tttttttttttttt                                           <  2:1865300/128‑1 (MQ=255)

                                                                                      ttAGAAAACTAATATCAAATTTTTAAAAAATTTTTAATTTTAAAAACTGATTTATTTTTCAAATTTTTAAATTATTTTAAAATAATTATAAAATAATCAATTTTTTGCTCt‑‑ttttttttttttttttt                                        <  1:8414101/128‑1 (MQ=255)

                                                                                           aaaCTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑TTTTTTTTTTTTTTTTaaaaaa                                   >  1:3605153/1‑128 (MQ=255)

                                                                                           aaaCTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑TTTTTTTTTTTTTTTTTAaaaa                                   >  1:1367287/1‑128 (MQ=255)

                                                                                              ctactaGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATCCTCG‑‑TTTTTTTTTTTTTTTTTAAAAAAat                                >  2:9645920/1‑128 (MQ=255)

                                                                                              attattttatttttttAAAAAATTTTTTATTTTTATAAATTAATTATTTTTAAAATTTTTATATTATTTTAAAATAATTATATTTTAATAAATTTTATTTTTG‑‑TTTTTTTTTTTTTTTTTACAAAAat                                <  1:2764192/118‑1 (MQ=255)

                                                                                               taaaataaattttttAAAAAATTTTTAATTTTAATAACTTTATTTTTTTTTAAATTTTTAAATTATTTTAAAATAATTATTTTTTAAAAAATTTTTTGTTGGT‑TTTTTTTTTTTTTTTTTACAAAAat                                <  1:8847046/119‑1 (MQ=255)

                                                                                                actaGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCa                                                                                     <  1:4722734/75‑1 (MQ=255)

                                                                                                actaGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑‑TTTTTTTTTTTTTTTAACAAAAataaa                             >  2:254536/1‑126 (MQ=255)

                                                                                                  tgaaattttttaAAAAAATTTTATTTTTATTTAATTTAATT‑TTTTTAAAATTTTTTAATTTTTTTAAAATCATTAATAATTAATAAATTTTATTCTCGTTTTTTTTTTTTTTTTTTTACAAAAAtata                             <  2:5311721/122‑1 (MQ=255)

                                                                                                   atcACTTTTTTAAAAAATTTTTTATTTTAAAAAATTAATTATTTTTAAAATTTTTAAATTATTTTAAAATCATTATAAATTAATCAATTTTATTCGCG‑‑TTTTTTTTTTTTTTTTTACAAAAATATAat                           <  2:2403661/126‑1 (MQ=255)

                                                                                                   ataaattttttAAAAAATTTTAAATTTTAATAATTGAATTATTTTTAATATTTTTAAATTTTTTTAAAATAATTATATATTAATAAATTTTATTCTCG‑‑TTTTTTTTTTTTTTTTTACAAAAATATAat                           <  2:4085328/123‑1 (MQ=255)

                                                                                                   aaaaCTTTTTTAAAAAATTTATAATTTTTAAATAAAAATTTATTTTCAAATTTTTTTATTTTTTTAAAATAATTATATTATAATAAATTTTTTTGTCG‑‑TTTTTTTTTTTTTTTTTACAAAAATATAat                           <  1:6033729/125‑1 (MQ=255)

                                                                                                   aGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCT‑‑TTTTTTTTTTTTTTTTAAAAAAAATaaaaa                           >  2:4618024/1‑127 (MQ=255)

                                                                                                   aGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑‑TTTTTTTTTTTTTTTTAAAAAAAAATAatt                          >  1:5520021/1‑128 (MQ=255)

                                                                                                   aGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGATCG‑‑TTTTTTTTTTTTTTTTTAAATAAATTTAat                           >  1:7384548/1‑128 (MQ=255)

                                                                                                    gCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑TTTTTTTTTTTTTTTTaaaaaaaaaaaattt                          >  2:10062322/1‑121 (MQ=255)

                                                                                                    gCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑TTTTTTTTTTTTTTTTTACAAAAAAATAatt                          >  2:311931/1‑128 (MQ=255)

                                                                                                     cACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATAAATTTTATGCTCGT‑TTTTTTTTTTTTTTTTTAAAAAAAAATAatt                          >  2:3004583/1‑128 (MQ=255)

                                                                                                      aCTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGT‑TTTTTTTTTTTTTTTTTAAAAAAATA‑Aattta                        >  2:3306297/1‑128 (MQ=255)

                                                                                                       cTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGT‑TTTTTTTTTTTTTTTTTACAAAAATATAatttt                        >  1:7929949/1‑127 (MQ=255)

                                                                                                       cTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑TTTTTTTTTTTTTTTTTAAAAAAATATAatttat                       >  1:9827985/1‑128 (MQ=255)

                                                                                                        ttttttAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑TTTTTTTTTTTTTTTTTACAAAAATATAatttatt                      >  1:8234875/1‑128 (MQ=255)

                                                                                                        ttttttAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑TTTTTTTTTTTTTTTTTAAAAAAATATAatttatt                      >  2:3906324/1‑128 (MQ=255)

                                                                                                        ttttttAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑TTTTTTTTTTTTTTTTAAAAAAAAT‑TAattttttt                     >  1:872581/1‑128 (MQ=255)

                                                                                                         tttttAAAAAATTTTTTATTTTAATAAATGAATTTTTTTTTAAATTTTATAATTATTTTAAAATCATTATAAATTAATAAAATTTAAGCTAG‑‑TTTTTTTTTTTTTTTTTACAAAAATATAatttattt                     <  1:6511934/128‑1 (MQ=255)

                                                                                                          ttttAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑TTTTTTTTTTTTTTTTTAAAAAAATAAAatttattta                    >  1:2455933/1‑127 (MQ=255)

                                                                                                             tAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑TTTTTTTTTTTTTTTTTACAAAAATATAATttattttatt                 >  1:8047553/1‑128 (MQ=255)

                                                                                                               aaaaaTTTTTAATTTTAATAAATTAATTTTTTTTTAAATTTTTTAATTTTTTTAAAATAATTTTTTATTTATAAATTTTTTTGTAGT‑TTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTa                <  1:9678144/128‑1 (MQ=255)

                                                                                                                aaaaTTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑TTTTTTTTTTTTTTTTTACAAAAATATAATTAATTTAATaaaa              >  2:9068216/1‑128 (MQ=255)

                                                                                                                aaaaTTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑TTTTTTTTTTTTTTTTTAAAAAAATATAATTTATTTTATTaaa              >  2:4418118/1‑128 (MQ=255)

                                                                                                                          atttaaatAACTTATATATTATTCAATTATTTAAATATATATAAAATCAATAAAAATTAATAAATTTTATGCCCG‑‑‑‑TTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTAAACGTATATTTATg  <  2:8170063/128‑1 (MQ=255)

||

ATTCATTCATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTAAAATTAAAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑TTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTAAACGTATATTTATG  >  CP016816/420038‑420289

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`