Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 516,362 T→C intergenic (+193/+313) JCVSYN2_02375 → / ← JCVSYN2_02380 antitermination protein NusG/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816516,3620TC99.2% 21979.6 / NA 5894intergenic (+193/+313)JCVSYN2_02375/JCVSYN2_02380antitermination protein NusG/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/5);  major base C (2668/3163);  minor base A (28/16);  total (2699/3195)
Fisher's exact test for biased strand distribution p-value = 2.20e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 5964 total aligned reads displayed.

AAAACATAAAATATATTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAAAAGAAAAATATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAATTTTTAAAATTTATTTTAATAAT  >  CP016816/516236‑516487
                                                                                                                              |                                                                                                                             
  aaCATAAAATATATTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACAtt                                                                                                                            >  2:7695027/1‑128 (MQ=255)
  aaCATAAAATATATTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACAtt                                                                                                                            >  1:2615791/1‑128 (MQ=255)
  aaCATAAAATATATTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGATTTTTTAAAGATTATTTAAATTTTTGTTTTTATTTTTAATTAAAATTTTAATAATAAAACAtt                                                                                                                            >  1:2060222/1‑128 (MQ=255)
   aCATAAAATATATTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACAttt                                                                                                                           <  2:9670561/128‑1 (MQ=255)
   aCATAAAATATATTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACAttt                                                                                                                           <  1:3211877/128‑1 (MQ=255)
      tAAAATATATTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAg                                                                                                                        >  1:8885054/1‑128 (MQ=255)
      aaaaaTATATTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTAATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACAATTTAg                                                                                                                        >  1:3368794/2‑128 (MQ=255)
       aaaaTATATTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGt                                                                                                                       >  1:2821894/1‑128 (MQ=255)
           tatatTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTAttt                                                                                                                   <  1:10003052/128‑1 (MQ=255)
           tatatTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTAttt                                                                                                                   <  2:9236795/128‑1 (MQ=255)
           tatatTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTAttt                                                                                                                   <  1:6757726/128‑1 (MQ=255)
            atatTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTg                                                                                                                  >  2:3692255/1‑128 (MQ=255)
             tatTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGc                                                                                                                 >  2:4073093/1‑128 (MQ=255)
             tatTTTTAAAAAATATGATTATATAAGATTAGAAAAAAAGCTTATTTTAAGGGATTTTTTAAATATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATCATAGTAttaac                                                                                                                 >  1:5413828/1‑125 (MQ=255)
              atTTTTATAAAAAATTTTTTTATAAGATTAGAAAAAAATTTGATTTTTATGTATTTTTTAAATATTATTTAACTTTTTTTTTTTATTTTTATTTAAAATTTTAATAAAAAAACATTTTATTAttttaa                                                                                                                <  1:6924858/128‑4 (MQ=255)
              atTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCa                                                                                                                <  2:5241879/128‑1 (MQ=255)
               tttttAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCaa                                                                                                               <  1:3588610/128‑1 (MQ=255)
               tttttAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCaa                                                                                                               <  2:7119228/128‑1 (MQ=255)
                ttttAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAAt                                                                                                              <  1:597283/128‑1 (MQ=255)
                 tttAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAAtt                                                                                                             <  2:2011921/128‑1 (MQ=255)
                  ttAAAAAATATGTTTTTATAAGATTAGTAAAAAAGCTTATTTTAAGGGATTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATta                                                                                                            <  2:6292691/128‑1 (MQ=255)
                    aaaaaaTATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATtata                                                                                                          >  2:8032200/1‑128 (MQ=255)
                     aaaaaTATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATaa                                                                                                         <  1:9291274/128‑1 (MQ=255)
                      aaaaTATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATaaa                                                                                                        <  2:9049150/128‑1 (MQ=255)
                       aaaTATGTTTTTATAAGATTAGAAAAAAAGCGTATATTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATCATaaac                                                                                                       <  1:6552752/128‑2 (MQ=255)
                           atGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGt                                                                                                   <  2:3507729/128‑1 (MQ=255)
                           atGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGt                                                                                                   <  2:7865538/128‑1 (MQ=255)
                              tttttATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTa                                                                                                <  2:1038526/128‑1 (MQ=255)
                              tttttATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTa                                                                                                <  1:7116198/128‑1 (MQ=255)
                                tttATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATg                                                                                              >  2:1538234/1‑128 (MQ=255)
                                tttATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATg                                                                                              >  1:2222893/1‑128 (MQ=255)
                                 ttaataGATTAGAATAAAAGCTTATTTTAATGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGa                                                                                             <  2:4703929/123‑1 (MQ=255)
                                 ttATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTAAtta                                                                                             >  1:4527047/1‑126 (MQ=255)
                                  tataAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGat                                                                                            >  1:6351808/1‑128 (MQ=255)
                                   ataAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGata                                                                                           >  2:10025080/1‑128 (MQ=255)
                                   ataAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGata                                                                                           >  2:9502308/1‑128 (MQ=255)
                                     aaGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGataat                                                                                         <  2:3350204/128‑1 (MQ=255)
                                      aGATTAGAATAAAAGATTATTTTAAGTGCTTTTTTAAAGATTATTTATAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGataata                                                                                        <  2:2547727/128‑1 (MQ=255)
                                      aGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGataata                                                                                        <  2:6490961/128‑1 (MQ=255)
                                      aGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGataata                                                                                        <  1:9484262/128‑1 (MQ=255)
                                       gATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAatat                                                                                       <  2:1670175/128‑1 (MQ=255)
                                         ttAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATAc                                                                                     <  1:4930209/128‑1 (MQ=255)
                                            gAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTa                                                                                  >  2:693632/1‑128 (MQ=255)
                                             aaaaaaaGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTAt                                                                                 <  2:5944629/128‑1 (MQ=255)
                                                aaaaGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTaa                                                                              <  2:10222016/128‑1 (MQ=255)
                                                 aaaGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTAATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATATGCTATTATAAAAAAGTTTATGATAATATAACTATTAat                                                                             >  2:4500979/1‑128 (MQ=255)
                                                 aaaGCTTATATTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTTCAATTATAAAAAAGTTTATGATAATATACCTATTAat                                                                             >  1:7484468/1‑128 (MQ=255)
                                                  aaGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATAACTATTAata                                                                            >  2:823687/1‑128 (MQ=255)
                                                   aGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAatat                                                                           >  2:672942/1‑128 (MQ=255)
                                                    gattATTTTAATTGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAAtata                                                                          <  2:5756039/126‑1 (MQ=255)
                                                    gCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAAtata                                                                          >  1:9077376/1‑128 (MQ=255)
                                                        aTTTTAAGTGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAAt                                                                      <  2:2200928/128‑1 (MQ=255)
                                                        aTTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAAt                                                                      <  1:3030164/128‑1 (MQ=255)
                                                         ttttAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATa                                                                     >  1:4714426/1‑128 (MQ=255)
                                                           ttAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATaaa                                                                   <  1:5260334/128‑1 (MQ=255)
                                                             aaGGGCTTTTTTAAAGATTATTTATAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATATTCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAatca                                                                 >  2:2919433/1‑128 (MQ=255)
                                                             aaGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAaaca                                                                 <  1:8486972/128‑1 (MQ=255)
                                                              aGGGCTTTTTTATAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAaacaa                                                                <  1:4171736/128‑1 (MQ=255)
                                                               gggCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAaacaac                                                               <  2:985953/128‑1 (MQ=255)
                                                               gggCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAaacaac                                                               <  1:1890970/128‑1 (MQ=255)
                                                                   ttttttAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGa                                                           <  1:157870/128‑1 (MQ=255)
                                                                    tttttAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGaa                                                          <  2:184526/128‑1 (MQ=255)
                                                                    tttttAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGaa                                                          >  1:5583121/1‑128 (MQ=255)
                                                                    tttttAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGaa                                                          <  1:6188291/128‑1 (MQ=255)
                                                                     ttttAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGaaa                                                         >  1:5080379/1‑128 (MQ=255)
                                                                      tttAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGaaaa                                                        <  2:4879908/128‑1 (MQ=255)
                                                                      tttAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGAAATTATAAAAAAGTTTATGATAATATACATATTAATATATAATAAAAAACTAGaaaa                                                        >  1:10468380/1‑128 (MQ=255)
                                                                      tttAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAACTTTAATAAAAAAAAATTTTAGTATTTGCAATTATAAATAATTTTATGATAATATACCTATTAATATATAATAAACAAATATaaaa                                                        >  2:3124161/1‑128 (MQ=255)
                                                                           aGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGaa                                                   >  2:5037805/1‑128 (MQ=255)
                                                                            gATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGaaa                                                  <  2:886409/128‑1 (MQ=255)
                                                                               tattTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAatat                                               >  1:5776207/1‑128 (MQ=255)
                                                                               tattTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAatat                                               >  2:6115370/1‑128 (MQ=255)
                                                                                attTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAAtatt                                              <  2:7537291/128‑1 (MQ=255)
                                                                                 tttAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAATCAACTAGAAAAGAGAAAtatta                                             >  2:2373835/1‑128 (MQ=255)
                                                                                   tAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATattatt                                           <  2:557572/128‑1 (MQ=255)
                                                                                     aaaTTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAg                                         >  2:5408052/1‑128 (MQ=255)
                                                                                        ttttGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTAt                                      <  2:429781/128‑1 (MQ=255)
                                                                                        ttttGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAAAAAATAGAAAAGAGAAATATTATTAGTAt                                      >  1:3763801/1‑128 (MQ=255)
                                                                                         tttGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATg                                     <  2:8461450/128‑1 (MQ=255)
                                                                                          ttGTTTTTATTTTTAATTAAAATTTTAATAAAAAAAGATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATAACTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGa                                    <  1:10253203/128‑1 (MQ=255)
                                                                                          ttGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGa                                    <  1:4354320/128‑1 (MQ=255)
                                                                                          ttGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGa                                    <  1:1186138/128‑1 (MQ=255)
                                                                                          ttGTTTTTAATATTAATTAAAATTTTAATAAAAAAACAATTAAGTATTTCCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGATAAAACAACTAGAAAAGAGAAATATTATTAGAATGa                                    >  1:2451725/1‑128 (MQ=255)
                                                                                              ttttatttttaATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGc                                <  2:1366623/128‑1 (MQ=255)
                                                                                               tttatttttaATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCg                               >  1:9852716/1‑128 (MQ=255)
                                                                                                 tatttttaATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGaa                             >  2:7300688/1‑128 (MQ=255)
                                                                                                 tatttttaATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGAGaa                             >  2:2711098/1‑128 (MQ=255)
                                                                                                  atttttaATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCTaat                            >  1:8305612/1‑128 (MQ=255)
                                                                                                  atttttaATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGaat                            <  1:8124619/128‑1 (MQ=255)
                                                                                                      ttaattaaAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGaattaat                        >  2:8659887/1‑128 (MQ=255)
                                                                                                         attaaAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAAATAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAAtttt                     <  1:8689055/128‑1 (MQ=255)
                                                                                                            aaaaTTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAATTTTTaa                  >  2:1142456/1‑128 (MQ=255)
                                                                                                              aaTTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAATTTTTaaaa                >  1:1394971/1‑128 (MQ=255)
                                                                                                                ttttAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAATTTTTAAAatt              <  1:7701103/128‑1 (MQ=255)
                                                                                                                 tttAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAATTTTTAAAattt             <  1:9652768/128‑1 (MQ=255)
                                                                                                                   taataaaaaaaaATTTAAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAATTTTTAAAataaat           <  1:7255481/122‑5 (MQ=255)
                                                                                                                      taaaaaaaCATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAATTTTTAAAATTTAtttt        <  1:1716238/125‑1 (MQ=255)
                                                                                                                         aaaaaCATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAATTTTTAAAATTTATTTtaat     >  1:3982760/1‑128 (MQ=255)
                                                                                                                          aaaaCATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAATTTTTAAAATTTATTTtaata    >  2:3851336/1‑128 (MQ=255)
                                                                                                                           aaaCATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAATTTTTAAAATTTATTTtaataa   >  1:7935661/1‑128 (MQ=255)
                                                                                                                              |                                                                                                                             
AAAACATAAAATATATTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAAAAGAAAAATATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAATTTTTAAAATTTATTTTAATAAT  >  CP016816/516236‑516487

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: