Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 484,791 T→A intergenic (‑100/+71) JCVSYN2_02220 ← / ← JCVSYN2_02225 hypothetical protein/50S ribosomal protein L7/L12

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816484,7910TA97.3% 13027.7 / 102.7 4419intergenic (‑100/+71)JCVSYN2_02220/JCVSYN2_02225hypothetical protein/50S ribosomal protein L7/L12
Reads supporting (aligned to +/- strand):  ref base T (55/66);  new base A (2014/2283);  total (2069/2350)
Fisher's exact test for biased strand distribution p-value = 7.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 4597 total aligned reads displayed.

TATTATTTAAAAAATAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTTAAAAAAAAAACAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATTTCT  >  CP016816/484664‑484913
                                                                                                                               |                                                                                                                            
       tAAAAAATAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAatataaaa                                                                                                                     >  1:8434543/1‑128 (MQ=255)
        aataaaTAGTAAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAatataaaa                                                                                                                     <  1:3916805/125‑1 (MQ=255)
         aaaaaTAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTAGATTATTTAATTATATTTTTTTTTAatataaaaa                                                                                                                    <  1:2281577/128‑1 (MQ=255)
          aaaaTAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAatataaaaag                                                                                                                   >  2:6411631/1‑127 (MQ=255)
              tAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAAATATATTTTTTTTAAATATAAAAAGCAAt                                                                                                               >  2:4564364/1‑128 (MQ=255)
                 tCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTaa                                                                                                            <  2:9369844/128‑1 (MQ=255)
                    aaaCTCATAGATTTAATCCTTTTATAGTTGCCCAAAAAATAAGATAGAGCATAGCTGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCTATTAAaca                                                                                                         >  1:4780565/1‑128 (MQ=255)
                    aaaCTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAaca                                                                                                         >  1:9890333/1‑128 (MQ=255)
                    aaaCTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAaca                                                                                                         >  1:3739479/1‑128 (MQ=255)
                    aaaCTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATAATATTTTTTTTTAATATAAAAAGCAATTAAaca                                                                                                         >  2:6766180/1‑128 (MQ=255)
                     aaCTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAacac                                                                                                        >  1:642744/1‑128 (MQ=255)
                       ctaaTAGATTTAATCCTTTTATAGTTGACCAAAAATTAAGATAGAGCATAGCAGAAGATCTTAACAACTAAAATAAACACGATAATTTAATTATATTTTTTTTTAATATAAAAAGAAATTAAaaacac                                                                                                      >  1:2648/4‑128 (MQ=255)
                       cTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTAATATAAAAAGCAATTAAAcacaca                                                                                                     >  1:1290342/1‑128 (MQ=255)
                       ataaTAGTTTTAATACTTTTATAGTTTACCAAAAAATAATCTAGAGCAAATCAGAAGCTCTTAACAAATAAACTACAATAGATTATTTAATTATATTTTTTTTTTAATATAAAAAGCAATTAAacaca                                                                                                       <  1:1872356/125‑1 (MQ=255)
                        tCATAGAATTAATACTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAAcacaca                                                                                                     <  1:4300383/128‑1 (MQ=255)
                          aTAGATTTAATCCTTTTATAGTTTACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACaaa                                                                                                   <  2:3137958/128‑1 (MQ=255)
                          aTAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACaaa                                                                                                   >  1:6961339/1‑128 (MQ=255)
                             gATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAAc                                                                                                <  2:900864/128‑1 (MQ=255)
                               tttAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTAAATTTTTTTTAAATATAAAAATAAATTAAACAAACAAAAAcaa                                                                                              >  2:1766382/1‑126 (MQ=255)
                               tttAATCCATTTATAGTTGACCAAAAACTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTc                                                                                              <  2:10081487/128‑1 (MQ=255)
                                ttAATCCATTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCa                                                                                             <  2:4402022/128‑1 (MQ=255)
                                 tAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTGAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATAAAACACACAAAAAcacaa                                                                                            >  2:8801814/1‑127 (MQ=255)
                                  aaTCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAcc                                                                                           <  2:6008945/128‑1 (MQ=255)
                                  aaTCCTTTTATAGTTGACCAAAAAATAAGATAGAGAATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAcc                                                                                           <  1:4480562/128‑1 (MQ=255)
                                   atacTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAcca                                                                                          <  1:7153210/125‑2 (MQ=255)
                                     ccTTTTATAGTTGAACAAAAAATAAGATAGAGAATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAccata                                                                                        <  2:4773034/128‑4 (MQ=255)
                                      cTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCAtt                                                                                       <  1:9436512/128‑1 (MQ=255)
                                        tttATAGATGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCATAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAccataatg                                                                                     <  2:7487983/128‑7 (MQ=255)
                                         ttATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATg                                                                                   <  2:2382971/128‑1 (MQ=255)
                                         ttATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATg                                                                                   >  1:8252592/1‑128 (MQ=255)
                                          tataGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATgg                                                                                  >  2:2958179/1‑128 (MQ=255)
                                          aataGTTGACCAAAAAATAAGATAGAGCATATCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAATCACTCAAAACCTCACCCATTAAgg                                                                                  <  2:5122660/127‑1 (MQ=255)
                                           atatTTGACCAAAAAATTAGATAGTTACTAGCAGTAGCGCTTAAAAACTAAACTACACTAGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGt                                                                                 <  1:5377654/128‑1 (MQ=255)
                                            taGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTg                                                                                <  1:5686554/128‑1 (MQ=255)
                                            taGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTg                                                                                >  2:6975990/1‑128 (MQ=255)
                                              gTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAccataatgggtgag                                                                               <  2:7125871/128‑13 (MQ=255)
                                                 gACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTGAATTATATTTTATTTTAATTTAAAAAGCAATTAAACACACAAAAACTCACCAATTATGTTTAGtta                                                                           >  2:10433642/1‑127 (MQ=255)
                                                  aCCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTa                                                                          <  1:780223/128‑1 (MQ=255)
                                                   ccAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTTAATATAAAAAGCAATTAAAAACACAAAAACACACCCATTATGTTAAGTTTa                                                                          >  1:166621/1‑128 (MQ=255)
                                                   ccAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAATCTCACCCATTATGGTGAGTTTaa                                                                         <  2:9603247/128‑1 (MQ=255)
                                                    caacaaATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTaaa                                                                        <  1:9192171/128‑1 (MQ=255)
                                                      aaaaaTAAGATAGAGCATAGCAGAAGGTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTaaaaa                                                                      <  1:8997/128‑1 (MQ=255)
                                                      aaaaaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAccat                                                                                         >  2:5472193/1‑108 (MQ=255)
                                                      aaaaaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTaaaaa                                                                      <  2:4251325/128‑1 (MQ=255)
                                                       aaaaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAat                                                                     >  1:6110168/1‑128 (MQ=255)
                                                        aaaTAAGATAGAGCATATCAGAATCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAatt                                                                    <  2:5678598/128‑1 (MQ=255)
                                                         aaTACGATGTAGCCTAGCAGAAGGTCATAACAACTAAACTACAATCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCATAATGGGTGAGtttttgtgtgt                                                                   <  1:6797004/128‑9 (MQ=19)
                                                             atatatatcATAGCAGAATCTATTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCa                                                               <  2:1531292/120‑1 (MQ=255)
                                                                 agagCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCaaaaa                                                           <  2:1159522/128‑1 (MQ=255)
                                                                  gagCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAc                                                                                            >  1:7580275/1‑95 (MQ=255)
                                                                  gagCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAAt                                                          >  2:5817948/1‑128 (MQ=255)
                                                                   agCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATg                                                                                   >  1:5020908/1‑102 (MQ=255)
                                                                   agCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAAtt                                                         <  2:3295138/128‑1 (MQ=255)
                                                                    gCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAAttt                                                        >  2:10274569/1‑128 (MQ=255)
                                                                       tAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGAAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGat                                                     <  1:4635943/128‑1 (MQ=255)
                                                                       tAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGAAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGat                                                     <  1:736544/128‑1 (MQ=255)
                                                                         gCAGAATCTCTTAACAACTAAACTACACTCGAATATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGatta                                                   <  2:4070933/128‑1 (MQ=255)
                                                                            gAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTATTTTAAAATGCAATTAAACACACAAAAAATCACCCATTATGGTGAGTTTAAAATTTATGCAAAAATTGGATTAttt                                                >  2:2174137/1‑128 (MQ=255)
                                                                            gAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTATAAATTATTCAAAAATTTGattatat                                                >  1:282815/1‑126 (MQ=255)
                                                                            gAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTAACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTAttt                                                >  2:8055336/1‑128 (MQ=255)
                                                                             aaGCTCTTAACAACTAAACTACACTCGATTATTTTATTATATTTTTATTAAATATAAAAAGCAATAAAACACACAAAAACTCAACCATAAATGTAAGATAAAAAATTATTCAAAAAATTGattattat                                               >  2:2586345/1‑126 (MQ=255)
                                                                             aaGCTCTTAAAAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTAtttt                                               <  2:8241694/128‑1 (MQ=255)
                                                                                  ctTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTTAGTTTAAAAATTATTCAAACATTTGCTTATTTAAAATc                                          >  1:2458075/1‑128 (MQ=255)
                                                                                  ctTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATc                                          >  1:6640657/1‑128 (MQ=255)
                                                                                  attAACAACTAAAGTACACTAGATTATATAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATc                                          <  2:6607968/127‑1 (MQ=255)
                                                                                   ttAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGTTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCa                                         >  2:9832842/1‑128 (MQ=255)
                                                                                   ttAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAATATTATACAAAAAATTGATTATTTTAAATCa                                         >  1:1493046/1‑128 (MQ=255)
                                                                                    tAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGt                                                                                 >  1:4146441/1‑87 (MQ=255)
                                                                                    tAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCaa                                        >  1:3177499/1‑128 (MQ=255)
                                                                                      acaacTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAAtt                                      <  1:10010442/128‑1 (MQ=255)
                                                                                      acaacTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATAAAACACACAAAAACTCACCCATTATGTTGATATTAAAAATTATTCAAAAAAATGATTAATATAAATCAAtt                                      >  2:7684895/1‑128 (MQ=255)
                                                                                        aactaaactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGa                                    >  2:8471311/1‑128 (MQ=255)
                                                                                        aactaaactaCAATCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGa                                    <  1:8811233/128‑1 (MQ=255)
                                                                                         actaaactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGaa                                   <  1:592954/128‑1 (MQ=255)
                                                                                         actaaactaCACTCGATTATTTAATTATATTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATACAAAACTTTGATTATTTTAAATCAATTGAAg                                  >  2:7288269/1‑128 (MQ=255)
                                                                                          ctaaactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAg                                  <  2:8677692/128‑1 (MQ=255)
                                                                                            aaaataCACTTTATAATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCt                                <  2:783960/128‑1 (MQ=255)
                                                                                             aactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCtg                               <  2:136032/128‑2 (MQ=255)
                                                                                             aactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTc                               >  2:3893485/1‑128 (MQ=255)
                                                                                               ctaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTTAAGCTCCa                             >  1:5192090/1‑128 (MQ=255)
                                                                                                taCACTCGCTTCTTTAATTATATTTTTTTTTACTATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAg                            <  2:1983154/128‑1 (MQ=255)
                                                                                                taCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAg                            <  1:3359699/128‑1 (MQ=255)
                                                                                                  cacTCGATTATTTAATTATATTTTTTTTTAAAAAAAAAAACAAATAAACCCACAAAAACTACCCCAATATGGTTAATTTAAAAATTAATAAAAAAATTTATTATTTTAAATACATTGAATCTCCAGca                          >  2:9203024/1‑127 (MQ=255)
                                                                                                  cacTAGATTATTTAATTATATTTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGc                           <  1:209676/128‑1 (MQ=255)
                                                                                                     tcttttattTAAATATATTTTTTTTTAATATAAAAAGCTATTAAACACACAAAAACTCACCCATTATGGAGATTTTAAAAATTATTCATAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCa                       <  1:5542499/124‑1 (MQ=255)
                                                                                                       gATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAAc                     <  2:5294183/128‑1 (MQ=255)
                                                                                                       gATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTAAAAAATTATTCAAAATTTTGATTATTTTAAATCAATTGAAGCTCCAGCTACaag                     >  1:10197952/1‑127 (MQ=255)
                                                                                                        attattTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACt                    >  1:9624431/1‑128 (MQ=255)
                                                                                                         ttattTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTa                   >  2:6181398/1‑128 (MQ=255)
                                                                                                            attAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAAtt                <  2:4949213/127‑1 (MQ=255)
                                                                                                              tAATTATATTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAg             <  2:3683198/128‑1 (MQ=255)
                                                                                                                aTTATATTTTTTTTTAAAATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAATATCCAGCTTCACCTAATTAAGc            >  1:8054455/1‑128 (MQ=255)
                                                                                                                 ttATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCt           <  2:3460444/128‑1 (MQ=255)
                                                                                                                 ttATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCt           <  2:2788489/128‑1 (MQ=255)
                                                                                                                   atatTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAATCTCCAGCTTCAACTAATTTAGCtta         >  1:10319109/1‑127 (MQ=255)
                                                                                                                   atatTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCttt         >  1:2985798/1‑128 (MQ=255)
                                                                                                                   atatTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAAATCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCttt         >  1:3582280/1‑128 (MQ=255)
                                                                                                                       ttttttttaATATAAAAAGCAATTAAACACACAAAAACTCACACATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCAtt     <  1:6232177/128‑1 (MQ=255)
                                                                                                                        tttttttaATATAAAAAGCAATTAAACACACAAAAACTCACACATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCAttt    <  1:8587157/128‑1 (MQ=255)
                                                                                                                          tttttaATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCCTttct  >  1:10504350/1‑128 (MQ=255)
                                                                                                                               |                                                                                                                            
TATTATTTAAAAAATAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTTAAAAAAAAAACAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATTTCT  >  CP016816/484664‑484913

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: