BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP016816	32,707	A→T	I1067F (ATT→TTT)	JCVSYN2_00105 →	ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP016816	32,707	0	A	T	99.3%	9746.8 / NA	3046	I1067F (ATT→TTT)	JCVSYN2_00105	ABC transporter permease
Reads supporting (aligned to +/- strand): ref base A (14/7); new base T (1472/1548); total (1489/1557)
Fisher's exact test for biased strand distribution p-value = 1.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 3146 total aligned reads displayed.

TGTTACTCAACCTTTAACTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTATTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACCTAAATCAAATAACATTGAAAC  >  CP016816/32580‑32834

tGTTACTCACCCTTTAACTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATtta                                                                                                                                 >  2:4789440/1‑128 (MQ=255)

  ttACTCAACCTTTAACTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCAtttttt                                                                                                                               >  1:1354290/1‑128 (MQ=255)

   tACTCAACCTTTAACTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTATTTTTAGGTTATTCTCCTTCATttttta                                                                                                                              >  2:1703478/1‑128 (MQ=255)

    aCTCAACCTTTAACTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTaa                                                                                                                             >  2:873627/1‑128 (MQ=255)

    aCTCAACCTTTAACTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTaa                                                                                                                             >  2:1804963/1‑128 (MQ=255)

    aCTCAACCTTTAACTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTaa                                                                                                                             >  1:3755357/1‑128 (MQ=255)

     cTCAACCTTTAACTAAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTTAAAAGAACAGATCTTAAACTTAATTTTATGTTATTATACTTCTTTTTTTaaa                                                                                                                            >  1:5471395/1‑128 (MQ=255)

      acacactatAACTCTGTATGTTGGATAACTTTTAAAAACTATTTATAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAAt                                                                                                                           <  1:4633292/120‑1 (MQ=255)

        aaCCTTTAACTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTaaa                                                                                                                            >  2:2688613/1‑125 (MQ=255)

        aaCCTTTAACTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCt                                                                                                                         <  1:112191/128‑1 (MQ=255)

          ccTTTAACTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTa                                                                                                                       >  1:4088922/1‑128 (MQ=255)

            tttAACTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTAtt                                                                                                                     >  2:3115393/1‑128 (MQ=255)

             ttAACTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTAttt                                                                                                                    <  1:1612317/128‑1 (MQ=255)

              tAACTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTa                                                                                                                   >  2:4440240/1‑128 (MQ=255)

                aCTCTGTTTCTTGGAGAACTTTTAAAAACTATTTATAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAAc                                                                                                                 <  1:5721540/128‑1 (MQ=255)

                aCTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAAc                                                                                                                 <  2:1373547/128‑1 (MQ=255)

                aCTCAGTATGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAAc                                                                                                                 <  2:1521158/128‑1 (MQ=255)

                    aGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCt                                                                                                                         >  2:4553033/1‑116 (MQ=255)

                    aGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGt                                                                                                             >  2:3270280/1‑128 (MQ=255)

                      tttGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAACTCTTATTTAACAAGTGa                                                                                                           >  2:7012722/1‑128 (MQ=255)

                      tttGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGa                                                                                                           >  2:750882/1‑128 (MQ=255)

                      tttGTTCGAGCACTTTTAAAAACTAATTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTAATCTCCTTCATTTTTTAACTCTGAGTTAACGAgaca                                                                                                           >  1:606441/1‑125 (MQ=255)

                          ttgGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTTTTTAACAAGTGAAtct                                                                                                       >  1:7216511/1‑128 (MQ=255)

                            ggAGAACTATTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCg                                                                                                     <  1:2892612/128‑1 (MQ=255)

                             gagaACTTTTAAAAACTATTTTTAAAGATAAAGTTCAAAGTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGa                                                                                                    <  1:4379465/128‑1 (MQ=255)

                             gagaACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGAGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGa                                                                                                    <  1:4218641/128‑1 (MQ=255)

                                aaCTTTTAAAATCTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAaga                                                                                                 <  2:7126972/128‑1 (MQ=255)

                                aaCTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAaga                                                                                                 <  1:5181493/128‑1 (MQ=255)

                                aaCTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAaga                                                                                                 >  2:4652485/1‑128 (MQ=255)

                                 aCTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAagag                                                                                                <  1:2125815/128‑1 (MQ=255)

                                  cTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGc                                                                                               <  1:3316402/128‑1 (MQ=255)

                                    tttAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTc                                                                                             <  1:5061364/128‑1 (MQ=255)

                                    tttAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTc                                                                                             >  2:6400889/1‑128 (MQ=255)

                                     ttAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAATAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCa                                                                                            <  1:5952473/128‑1 (MQ=255)

                                     ttAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCa                                                                                            <  1:2494519/128‑1 (MQ=255)

                                       aaaaaCTATTTTTAAAGATAAAGAGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAAt                                                                                          <  2:5652142/128‑1 (MQ=255)

                                        ataaCTATTTTTATAGATAAAGTGCAAACTACAGGTTAGTGAAAATAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGATTCTCGAAGAGCTCAAtt                                                                                         <  1:225892/126‑1 (MQ=255)

                                          aaCTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAAtttt                                                                                       >  2:2057135/1‑128 (MQ=255)

                                           aCTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTc                                                                                      <  2:6805960/128‑1 (MQ=255)

                                           aCTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTc                                                                                      >  1:8283/1‑128 (MQ=255)

                                             tATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCtt                                                                                    <  2:5235852/128‑1 (MQ=255)

                                                 tttAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTgg                                                                                <  1:263221/128‑1 (MQ=255)

                                                 tttAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTgg                                                                                >  2:4960846/1‑128 (MQ=255)

                                                    aaaGATAAAGAGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGATATACTCCTTCAATTTTTAAATATTATTTAAAAAGTGAATCTCGAAGAGCTCAATTTTCTTATAGTTg                                                                             >  2:5902258/1‑128 (MQ=255)

                                                     aaGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGt                                                                               >  1:2356087/1‑125 (MQ=255)

                                                      aGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGAGAATCTCGAAGCGCTCAATTTTCTTTTGGTTGAc                                                                           >  2:2951990/1‑128 (MQ=255)

                                                       gATTAAGTGCTAACTACAGGTGAGTGAAATGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACa                                                                          <  2:5113967/128‑1 (MQ=255)

                                                          aaaGTGCAAACTACAGGTGAGTGAAAAGAACAGATCTTTAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACaaaa                                                                       <  2:6527296/128‑1 (MQ=255)

                                                          aaaGGGCCAACTTCAGGTGAGTGAAATGAATAGATCTTAAACTAAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACaaaa                                                                       <  2:3635555/128‑1 (MQ=255)

                                                           atcttctaaCTATAGGTGAGTGATAATAAAAGATCTTAAACTTAATTTTAGGTTTTTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACaaaaa                                                                      <  1:386603/121‑1 (MQ=255)

                                                           aaGTGCAAACTACAGGTTAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACaaaaa                                                                      <  2:2318885/128‑1 (MQ=255)

                                                           aaGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACaaaaa                                                                      <  1:6946737/128‑1 (MQ=255)

                                                                  taCTTCAGGTGAGTGAAAAGAATAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACta                                                               <  1:1476491/127‑1 (MQ=255)

                                                                  aaCTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACta                                                               <  2:3532224/128‑1 (MQ=255)

                                                                   aCTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACtat                                                              >  2:6178692/1‑128 (MQ=255)

                                                                      aCAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACATACTATAAt                                                           >  1:7079750/1‑128 (MQ=255)

                                                                        aGGTGAGTGAAAAGAAAAGATCTTAAACTTAATATTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGCTTGACAAAAACAAACTATAATTc                                                         >  1:6653619/1‑128 (MQ=255)

                                                                         ggTGAGTGATAAGAAAAGATCTTAAACTTTATTTTAGGTTATTCTCCATCATTTTTTATATCTTTTTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAAGAAACTATAATTcc                                                        <  1:6519808/128‑1 (MQ=255)

                                                                         ggTCAGTGACAAGTAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTcc                                                        <  1:3171108/128‑1 (MQ=255)

                                                                           ttagtgaATAGAAAAGATCTTAAACTTAATTTTTTGTTATTCTCCTTCATTTTTTATATCTTATTTAACAAGTGATTGTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAATCAAACTATAATTCcac                                                      <  1:1969981/126‑1 (MQ=255)

                                                                              gtgaAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACaaa                                                   >  2:6929973/1‑128 (MQ=255)

                                                                               tgaAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAACACCAAACTATAATTCCACaaaa                                                  >  1:398561/1‑128 (MQ=255)

                                                                               tgaAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACaaaa                                                  <  1:5812725/128‑1 (MQ=255)

                                                                                gaaaagaaaaGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACaaaaa                                                 >  1:4906119/1‑128 (MQ=255)

                                                                                   aagaaaagaTCTTAAACTTCATCTTAGGTTATTCTGCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGAt                                              <  1:4758669/128‑1 (MQ=255)

                                                                                   aagaaaagaTCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGAt                                              <  2:6283358/128‑1 (MQ=255)

                                                                                     gaaaagaTCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCa                                            <  2:4182736/128‑1 (MQ=255)

                                                                                      aaaagaTCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCaa                                           <  1:5321600/128‑1 (MQ=255)

                                                                                       aaagaTCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACt                                                                <  1:1224609/106‑1 (MQ=255)

                                                                                         agaTCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTTTTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTa                                        >  1:3430531/1‑128 (MQ=255)

                                                                                         agaTCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTa                                        <  1:1000162/128‑1 (MQ=255)

                                                                                         agaTCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTATTTTTTGACAAAAACAAACTATAATTCCACAAAAAGATAAATTa                                        >  2:2829194/1‑128 (MQ=255)

                                                                                         agaTCTTAAACTTAATATTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTa                                        <  1:3014391/128‑1 (MQ=255)

                                                                                          gaTCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAg                                       <  2:1957430/128‑1 (MQ=255)

                                                                                             cTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCAACAAAAAGATCAATTAGCaa                                    >  2:5766511/1‑128 (MQ=255)

                                                                                              ttAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAAc                                   >  2:4040827/1‑128 (MQ=255)

                                                                                               tAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACt                                  <  2:6637206/128‑1 (MQ=255)

                                                                                                aaaCTTAATTTTATGTTAATCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTa                                 <  2:5502503/128‑1 (MQ=255)

                                                                                                aaaCTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTa                                 <  2:4328121/128‑1 (MQ=255)

                                                                                                   cTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTAttt                              >  2:2472968/1‑128 (MQ=255)

                                                                                                     tAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGTTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTAttttt                            >  1:4533603/1‑128 (MQ=255)

                                                                                                          ttAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCcac                                                      >  2:2209097/1‑97 (MQ=255)

                                                                                                           tAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACCt                      >  2:1224453/1‑128 (MQ=255)

                                                                                                           tAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACCt                      >  2:5351710/1‑128 (MQ=255)

                                                                                                            aGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAaaac                                                                     >  1:1742641/1‑80 (MQ=255)

                                                                                                            aGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACCTa                     <  1:6159668/128‑1 (MQ=255)

                                                                                                            aGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACCTa                     >  2:376996/1‑128 (MQ=255)

                                                                                                             ggTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACCTaa                    >  1:5611088/1‑128 (MQ=255)

                                                                                                               ttATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACCTAAAt                  <  2:2588741/128‑1 (MQ=255)

                                                                                                                taatctcCTTCATTTTATAACTCTAATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCATTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACCTAAATc                 <  1:6262057/125‑1 (MQ=255)

                                                                                                                  ttCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACCTAAATCaa               >  1:6822618/1‑128 (MQ=255)

                                                                                                                  ttCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACCTAAATCaa               <  1:6390311/128‑1 (MQ=255)

                                                                                                                       cTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTTGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACCTAAATCAAATAAc          <  2:6054162/128‑1 (MQ=255)

                                                                                                                         tgaTTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACCTAAATCAAATAACAt        <  1:979354/126‑1 (MQ=255)

                                                                                                                         tCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACTAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACCTAAATCAAATAACAt        <  2:3391655/128‑1 (MQ=255)

                                                                                                                         tCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACCTAAATCAAATAACAt        <  1:2752466/128‑1 (MQ=255)

                                                                                                                         tCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACCTAAATCAAATAACAt        <  2:3899455/128‑1 (MQ=255)

                                                                                                                          cATTTTTTAAATCTTATTTAACAAGTGAATGTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACCTAAATCAAATAACAtt       <  1:3585231/128‑1 (MQ=255)

                                                                                                                           aTTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACCTAAATCAAATAACATTg      >  2:1109696/1‑128 (MQ=255)

                                                                                                                            ttttttAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACCTAAATCAAATAACATTGa     <  1:1866271/128‑1 (MQ=255)

TGTTACTCAACCTTTAACTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTATTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACCTAAATCAAATAACATTGAAAC  >  CP016816/32580‑32834

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`