Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 165,268 (T)19→18 intergenic (+232/‑310) JCVSYN2_00665 → / → JCVSYN2_00670 tRNA N6‑adenosine(37)‑N6‑threonylcarbamoyltransferase complex dimerization subunit TsaB/tRNA‑Ser

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816165,2500T.81.5% 4951.4 / inf 1904intergenic (+214/‑328)JCVSYN2_00665/JCVSYN2_00670tRNA N6‑adenosine(37)‑N6‑threonylcarbamoyltransferase complex dimerization subunit TsaB/tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base T (152/198);  new base . (938/606);  total (1090/814)
Fisher's exact test for biased strand distribution p-value = 5.17e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 2374 total aligned reads displayed.

ATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTAAAAGAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTA‑GGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATGTTAATTAAAAC  >  CP016816/165123‑165377
                                                                                                                               |                                                                                                                                
 taataTTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGATCTTGTAAAAAATAGCta                                                                                                                                 >  1:5038042/1‑127 (MQ=255)
   atatTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttt                                                                                                                               >  2:5772781/1‑128 (MQ=255)
     atTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttt                                                                                                                             >  1:7214851/1‑128 (MQ=255)
     atTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAAATTGTAAAAAAAATAtttttt                                                                                                                             >  2:3369921/1‑128 (MQ=255)
       ttATTTTAAATAATTTATCGCAATTTTATGAATTATTAAGAAACTAAAAAAATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttt                                                                                                                           <  2:2625220/128‑1 (MQ=255)
          gTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttt                                                                                                                        >  2:7173977/1‑128 (MQ=255)
           tttCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATATCtttttttttttt                                                                                                                       >  2:5370585/1‑128 (MQ=255)
             tCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttt                                                                                                                     >  2:3054936/1‑128 (MQ=255)
              cATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATATCttttttttttttttt                                                                                                                    >  2:687636/1‑128 (MQ=255)
              cATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTCTAAAAAATAGCttttttttttttttt                                                                                                                    >  1:3048992/1‑128 (MQ=255)
              aaTTAATTTATCGCACTTGTATTAATTAGTAATAAAATAAAACTATTATATTTTTATATATTTTTTTTATATTCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAAAAGCttttttttttttttt                                                                                                                    <  2:1859674/127‑1 (MQ=255)
               aTTAATTTATATCAATTTTATTAATTAGTAATAAAATAAAAATATTATCTTTTTAGATATTTTTTTTATTTTAAAATTTTTAAAAAATTAATTTTAAATTGTAAAAAATATCtttttttttttttttt                                                                                                                   <  1:4342220/128‑1 (MQ=255)
                   aTTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttttttaat                                                                                                               >  1:1148497/1‑125 (MQ=255)
                    tttATCGAACTTTTATGAATTATTAATAAACTAAAAATATTATATTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATACCttttttttttttttttataat                                                                                                              <  2:2835923/128‑6 (MQ=255)
                     ttATCTCACTTTTATTTATTTTTAATAAAAAAAAACTATTATCTTTTTATTTATTTTTTTTATATTCAAATTTTTAAAAAATTAATTTTAAATTGTAAAAAATAGCttttttttttttttttataatg                                                                                                             <  2:5969351/128‑7 (MQ=255)
                      tttCGCAATTGTATGAATTTGTAATAAACAAAAAATATTATATTTTTATAAATTTTTTTTTTATTCAAATTTTTAAAAAAATAAATTGAACTTGTAAAAAAAAGCttttttttttttttttataatgg                                                                                                            <  2:1316581/126‑8 (MQ=255)
                       aTCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTtataattt                                                                                                           >  1:475115/1‑124 (MQ=255)
                       aTCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTtatgga                                                                                                           >  1:876963/1‑125 (MQ=255)
                         cGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttttataaaatgaaaa                                                                                                         >  1:3604111/1‑116 (MQ=255)
                              ttttatttattattAATAAAATAAAAATATTATATTTTTTTATATTTTTTTTTTTTTCAAATTTTTAAAAAATTAATTTTAAATTTTAAAAAATACC‑‑TTTTTTTTTTTTTTTTTATAATGGAAAtata                                                                                                  <  2:4745734/120‑1 (MQ=255)
                              ttGTATTTATTATTATTAAAATAAAAATATTTTATTTTTATATATTTTTTTTATATTAAAATTTTTAAAAAATTAATTTTAACTTTTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATaa                                                                                                 <  1:228488/128‑1 (MQ=255)
                               tGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTATAAttaatttaat                                                                                                  >  1:2875933/1‑119 (MQ=255)
                                gTATTAATTATTAAGAAAATAAAAATATTATTTTTTTAGTTATTTTTTGTAAATGCAAATTTTTAAAAAATTAATTTTAACTTGTAAAAAATA‑‑‑‑TCTTTTTTTTTTTTTTTATAATGGAAATATAAAtt                                                                                              <  1:5771896/128‑1 (MQ=255)
                                 tattaattattaatAAACTAAAAATATTATCTTTTTATAATTTTTTTTTATATTTAAATTTTTAAAAAAATAATTTTAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAAtt                                                                                              <  2:3737567/128‑1 (MQ=255)
                                    taaTTATTAATAAAATAAAAATATTATATTTTTATATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTTAAATTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTa                                                                                           <  2:193197/127‑1 (MQ=255)
                                     aaatacgaaGATACTAAAAAAATTATATTTTTTTATAATTTTTTTATTTGCAAATTTTTAAAAAATTAATTTGTACTTTTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTa                                                                                           <  1:4891685/121‑1 (MQ=255)
                                        tAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATTTAAATATAATTTTTA‑gttt                                                                                      >  1:1927753/1‑125 (MQ=255)
                                        tAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTattta                                                                                       >  2:4823671/1‑124 (MQ=255)
                                         tttaattaaataaaaataTTTTATTTTTTTATATTTTTTTTTTATTGAAATTTTTTAAAAATTATTTTTTAATTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTA‑GGTAt                                                                                     <  1:4480013/118‑1 (MQ=255)
                                         aGTAATAAAATAAAAATTTTATTTTTTTATATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTTAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTA‑GGTAt                                                                                     <  2:774713/128‑1 (MQ=255)
                                          ttattaaaataaaaataTTTTTTTTTTATATATTTTTTTTATTTTAAAATTTTTAAAAAATTAATTTTAAATTTTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTA‑GGTAt                                                                                     <  2:1440481/123‑1 (MQ=255)
                                          gTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTGAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGTAAATATATATTTta‑tttatt                                                                                    >  2:6094057/1‑128 (MQ=255)
                                            aaGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATTGAAATAAAAATTTTA‑GTTAtttt                                                                                  >  1:7090617/1‑128 (MQ=255)
                                            aaGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATA‑‑‑TCTTTTTTTTTTTTTTTTATAATTTAAAAATAAATTTTA‑TTTAtttt                                                                                  >  2:3481489/1‑128 (MQ=255)
                                               aaactaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTAATTAT‑‑T‑GAAATAAAAATTTTATGTTAtttttt                                                                                >  1:3470723/1‑126 (MQ=255)
                                                  ctaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTA‑AATGGAAATATAAATTTTA‑GTATTTTTTTTGTCt                                                                           >  2:4982257/1‑128 (MQ=255)
                                                   taaaattaTTTTTTTTTTTTTTATTTTTTTTTTATTTAAATTTTTAAAAAATTAATTTTAAATTTTAAAAAAATGC‑‑‑‑‑TTTTTTTTTTTTTTATAATGGAAATATAAATTTTA‑GGTATTTTGTTGTCTaa                                                                         <  2:6463959/128‑1 (MQ=255)
                                                   taaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTT‑‑‑‑TAATGTAATTATAAATTTTA‑GGTATTTTTTTGTCTa                                                                          >  1:5474547/1‑128 (MQ=255)
                                                   taaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTAAAATTAAAATTTAAATTTTA‑TTTATtttttttt                                                                             >  1:1823161/1‑126 (MQ=255)
                                                   taaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTATAATTTAAATATAAATTTTA‑GTTTTTttgttttata                                                                          >  1:3768593/1‑123 (MQ=255)
                                                    aaatattttttttttttATATATTTTTTTTTAATTTAAATTTTTAAAAAATTAATTTTAACTTTTAAAAAATA‑‑‑‑‑TCTTTTTTTTTTTTTTATAATGGAAATATAAATTTTA‑GGTATTTTGTTGTCTAAg                                                                        <  2:6794683/116‑1 (MQ=255)
                                                    aaaaattTTATTTTTTTATATATTTTTTTTATTTTTAAATTTTTAAAAAATTAATTTTTACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTA‑GGTATTTTGTTGTCTa                                                                          <  2:4449684/128‑1 (MQ=255)
                                                     aaaataTTATATTTTTATTTATTTTTTGTATATGCAAATTTTTAAAAAATTAATATGAACTTGTAAAAAAAAGC‑‑‑‑TTTTTTTTTTTTTTTATAATGGAAATATAAATTTTA‑GGTATTTTGTTGTCTAAg                                                                        <  2:4624788/128‑1 (MQ=255)
                                                      aattattttTTTTTTTTAAATTTTTTTTAATTTAAATTTTTAAAAAAATTATTTTTAA‑TTTTAAAAAAAA‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTA‑GGTATTTTGTTGTCTAAg                                                                        <  2:5892728/125‑1 (MQ=255)
                                                       aatattatTTTTTTTTTTATTTTTTTTATATTTAAATTTTTTAAAAATTTATTTTTATTTTTAAAAAATA‑‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTA‑GGTATTTTGTTGTCTAAg                                                                        <  1:3297223/126‑1 (MQ=255)
                                                         tattatCTTTTTTTTTATTTTTTGTTTATTCAAATTTTTAAAAAAATTATTTGAACTTTTTAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTATAATGGAAATATAAATTTTA‑GGTATTTTGTTGTCTAAGTATc                                                                    <  1:2407777/128‑1 (MQ=255)
                                                         tattatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTT‑‑‑TAATTGAAATATAAATTTTA‑TTTTTTTTTTTGTATAATTAt                                                                     >  1:2026853/1‑128 (MQ=255)
                                                         tattatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATTAAAATAAAAATTTTA‑ATTATttttttttaaaattat                                                                     >  1:2640837/1‑118 (MQ=255)
                                                          attatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTA‑ATTATtttttttataaattata                                                                    >  1:6824611/1‑117 (MQ=255)
                                                               cTTTTTAGATTTTTTTTGTATTTGCAAATTTTTAAAAAATTAATTTTAAATTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTA‑GGTATTTTGTTGTCTAAGTATCTaaaa                                                               <  2:6298048/128‑1 (MQ=255)
                                                                 ttttATATATTTTTTTTATATGCAAATTTTTTAAAAATTTATTTGAACTTTTAAAAAATA‑‑‑‑TCTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTA‑GGTATTTTGTTGTCTAAGTATCTaaaaaaa                                                            <  2:3986821/128‑1 (MQ=255)
                                                                 ttttATATATTTTTTTTATAATCAAATTTTTAAAAAATTAATTTTAACTTTTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTA‑GGTATTTTGTTGTCTAAGTATCTaaaaaa                                                             <  2:901171/128‑1 (MQ=255)
                                                                 ttttAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTATTTTTTTTTATTTATAATGTAATTATAATTTATA‑GGTATTATGTTGTATAAGTATCAaaaaaa                                                             >  1:5623589/1‑128 (MQ=255)
                                                                  tttAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTA‑TTTATTTTTTTGTCTAATTATAaaaaaaaa                                                            >  2:2316713/1‑128 (MQ=255)
                                                                  tttAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTATATAATGAAAAAATAAATTTTA‑TTTATTTTTTTGTCTATTTATATaaaaaaa                                                            >  2:3288715/1‑128 (MQ=255)
                                                                   ttATATATTTTTTTTATATTCAAATTTTTAAAAAATTAATTTGAAATTGTAAAAAATAG‑‑‑ATTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTA‑GGTATTTTGTTGTCTAAGTATCTaaaaaact                                                           <  2:6962717/128‑3 (MQ=255)
                                                                    ttattatTTTTTTTTAAAGTAAATTTTTAAAAAAATAATTTTTAATTTTAAAAAA‑A‑‑TTCTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTA‑GGTATTTTGTTGTCTAAGTATCTAAAAAAATa                                                          <  2:6564928/124‑1 (MQ=255)
                                                                    tatattttttttGTATATTCAAATTTTTAAAAAATTAATTTTAACTTGTAAAAAATAGC‑‑‑‑‑TTTTTTTTTTTTTTATAATGGAAATATAAATTTTA‑GGTATTTTGTTGTCTAAGTATCTAAAAAAATAGa                                                        <  2:5674169/122‑1 (MQ=255)
                                                                    taatttttttttttttATTAAAATTTTTATA‑AATTTATTTTTAATTTTTAAAAATATTTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTA‑GGTATTTTGTTGTCTAAGTATCTaaaaaaa                                                            <  2:3622246/122‑1 (MQ=255)
                                                                      tatatTTTTTTTATATTCAAATTTTTAAAAAATTAATTTGAACTTTTAAAAAAT‑‑‑TTCTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTA‑GGTATTTTGTTGTCTAAGTATCTAAAAAAATAGa                                                        <  1:6031596/127‑1 (MQ=255)
                                                                       atatTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTA‑TTTATTTTTTTATATAATAAAAAAAAAAAATATaa                                                       >  1:1560498/1‑128 (MQ=255)
                                                                          ttttttttttATGTAAATTTTTAAAAAATTAATTTTAAATTTTTAAAAATA‑‑‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTA‑GGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAgg                                                    <  2:316038/128‑1 (MQ=255)
                                                                              ttGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTA‑TTTATTTTTTTGTCTAATTATATAAAAAAATATAAAGGaaaa                                                >  2:4148045/1‑128 (MQ=255)
                                                                                ttataTGTATATTTTTAAAAAATTTATTTTAAATTTTAAAAAATA‑‑‑‑TATTTTTTTTTTTTTTTATAATGGAAATATAAATTTTA‑GGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAat                                             <  2:1017006/127‑1 (MQ=255)
                                                                                gTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTA‑AATGTAAATATAAATTTTA‑GTTATTTTGTTTTATAATTATATAAAAAAATATAAAGTaaaaa                                               >  2:2475405/1‑128 (MQ=255)
                                                                                  atatGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTATAATTTAAATAAAAATTTTA‑TTTATTTTTTTTTATATTTTTATAAAAAAATATAAATTaaaaaa                                              >  2:2727507/1‑128 (MQ=255)
                                                                                     tGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATTAAATTAAAAATTTTA‑TTATTTTTTTTTTAAAATTATAAAAAAAAATATATAGAAAAATAtttt                                          >  2:5149714/1‑128 (MQ=255)
                                                                                     tGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTAAAATGTAAATATAAATTTTA‑TGTATTTTTTTTTATAAGTA‑AAAAAAAAATATAAAGGaatatcgtatg                                         >  2:3866015/1‑119 (MQ=255)
                                                                                     tGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATTGAAATATAAATTTTT‑GTTATTTTTTTTTCTATTTATAAAAAAAAATAAATATGaaaaatatttt                                         >  1:6264034/1‑122 (MQ=255)
                                                                                     tGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTAAAAAGAAAATATAAATTTTA‑TTTATTTTTATTAAAAAAAATATAAAAAAATAAAAATTaaaaaaaaata                                         >  1:5925527/1‑123 (MQ=255)
                                                                                       taaaTTTTTAAAAAATTATTTTTAA‑TTTTTAAAAATA‑‑‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTA‑GGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTa                                      <  1:5194687/127‑1 (MQ=255)
                                                                                       cAAATTTTTAAAAAATTAATTTTTACATTTAAAAAA‑A‑TTCTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTA‑GGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGt                                        <  1:6395379/128‑1 (MQ=255)
                                                                                        aaaTTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTA‑AATGTAAAAATAAATTTTA‑TGATTTTTTTTTTAAATTTATAAAAAAAAATAAAAATTaaaaaaatatttaa                                      >  1:2744581/1‑120 (MQ=255)
                                                                                        aaaTTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATATTGTAAATATAAATTTTA‑GTTATTTTTTTTTATAAGTATATAAAAAAATAAAAAGTAAAAAATAttttaa                                      >  1:1335864/1‑124 (MQ=255)
                                                                                        aaaTTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTA‑GTTATTTTTTTGTCTAAGTATCAAAAAAAATAGAAAGTAAAAAATTTTTTTa                                      >  1:390340/1‑128 (MQ=255)
                                                                                          tttttTAAAAAATTAAATTTAAATTGT‑AAAAAT‑‑‑TTTCTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTA‑GGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTaaaa                                   <  2:1569036/127‑1 (MQ=255)
                                                                                            tttAAAA‑AATTAATTTTAAATTTT‑‑AAAATA‑ATTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTA‑GGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGt                                 <  2:2063055/128‑1 (MQ=255)
                                                                                              ttaaaaaaTTTATTTTAAATTGTTAAAAATA‑‑‑TCTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTA‑GGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTa                                <  2:5505430/126‑1 (MQ=255)
                                                                                                aaaaaaTTTATTTTTAATTTTTAAAAATTTTTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTA‑GGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAACAATATTGTTAAAAGt                                 <  2:6677929/128‑1 (MQ=255)
                                                                                                aaaaaaTTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATTGAAATATAAATTTTA‑GGTATTTTTTTGTATAATTATATAAAAAAAAAAAAAGTAAAAAATATTTTAAAAATTaaa                              >  1:5353636/1‑127 (MQ=255)
                                                                                                  aaaattaattTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTA‑TTTATTTTGTTTTATAATTATATAAAAAAATATAAAGTAAAAAATATTTTTAAAATTataaa                            >  2:5306324/3‑124 (MQ=255)
                                                                                                     attaattTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTA‑GGTATTTTGTTTTATAATTATCTAAAAAAATATAAAGTAAAAAATATTGTTAAATGTAATACTaa                         >  1:696154/1‑128 (MQ=255)
                                                                                                     attaattTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTA‑GGTATTTTGTTGTCTAAGTATATAAAAAAAAAGAAAGGAAAAAATTTTGTTAAAATAAAAACTaa                         >  1:6138935/1‑128 (MQ=255)
                                                                                                     attaattTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTATAATTTAAATAAAAATTTTA‑GGAATTTTTTTTTATAATTATATAAAAAAATATAAAGTAAAAAATATTGTTAAAATTAAAAATaaa                        >  1:4216562/1‑128 (MQ=255)
                                                                                                      ttaattTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTTAAATTTAAATAAAAATTTTA‑TTTTTTTTTTTTTATAAATATAAAAAAAAATAAAAATGAAAAAATATTGTAaaaataaaaaaa                           >  1:6677929/1‑120 (MQ=255)
                                                                                                      ttaattTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAAAAAAATTTTTT‑GTTTTTTTTTTTTATAATTAAATAAAAAAAAAAAAAGAAAAAATAATTGTTAAAATTaaaaaaaaa                        >  1:3183833/1‑121 (MQ=255)
                                                                                                       taattTGTACTTGTTAAAAATA‑‑‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTA‑GGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAATACTAAAat                      <  2:2198274/128‑1 (MQ=255)
                                                                                                        aattTGAAATTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTA‑GGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAATACTAAAat                      <  2:6198577/128‑1 (MQ=255)
                                                                                                         attTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTATATA‑‑GGAAATATAAATTTTA‑GGTATTTTGTTGTAAAATTATCTAAAAAAATAGAAAGTAAAAAATATTGTTAAaataaataaaaaatt                      >  1:6556230/1‑121 (MQ=255)
                                                                                                         attTGAACTTGTAAAAAATAGC‑‑‑‑‑TTTTTTTTTTTTTTATAATGTAAATATAAATTTTA‑GTTTTTTTTTTTTTTAATTATAAAAAAAAAAATAATGAAAAAAATTTTATAAAAATAAATAATAAaataat                   >  1:4632286/1‑128 (MQ=255)
                                                                                                              atattttAAAAAATATC‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTA‑GGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTAttt                 <  1:2123324/122‑1 (MQ=255)
                                                                                                              aaCTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAGTGGAAATATAAATTTTA‑GGTATTTTGTTGTCTAAGTATCTAAAAAAATATAAAGGAAAAAATATTTTTAAATGTAATACTAAAtttattta                >  2:7094910/1‑128 (MQ=255)
                                                                                                              aaCTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTA‑GTTATTTTTTTGTATAAGTATATAAAAAAATATAAATTAAAAAATATTTTTAAAATTAATACTAAAATTATTTa                >  2:2921750/1‑128 (MQ=255)
                                                                                                                cTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTAAAATGTAAATATAAATTTTA‑GTTATTTTTTTGAAAAATAATATAAAAAAAAATAAATGAAAAAAAATATAAAAAATTAAAACTAAAATtaattaat              >  1:1690284/1‑128 (MQ=255)
                                                                                                                 ttGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTATAATGGAAATATAAATTTTA‑GGTATTTTGTTGTATAAGTATCTAAAAAAATATAAAGGAAAAAATATTGTTAAAATTAATTCTAAAATTATTTAATGt            >  2:1169139/1‑128 (MQ=255)
                                                                                                                  tGTAAAAAATAGC‑‑‑TTTTTTTTTTTATTTTATAATGGAAATATAAATTTTA‑GGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTa                >  1:146288/1‑124 (MQ=255)
                                                                                                                   attgtaaaaaattttttttttttttttttttATAATGGAAATATAAATTTTA‑GGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAAt              <  1:4032383/116‑1 (MQ=255)
                                                                                                                    tAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTA‑GGTATTTTGTTGTATAATTATATAAAAAAATATATATTAAAAAATATTTTTAAAAGTAATAATAAAATTATTTAAttta           >  2:1697209/1‑125 (MQ=255)
                                                                                                                    tAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATTGAAATTTAAATTTTA‑GGTATTTTGTTGTATAAGTATATAAAAAAATATAAAGTAAAAAATATTTTTAAAATTAATAATAAAATTATTTAATTtta          >  2:4296839/1‑128 (MQ=255)
                                                                                                                        aataatttttttttttttttttttttATAATGGAAATATAAATTTTA‑GGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATGttaa         <  1:1005385/121‑1 (MQ=255)
                                                                                                                               |                                                                                                                                
ATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTAAAAGAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTA‑GGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATGTTAATTAAAAC  >  CP016816/165123‑165377

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑