Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 165,222 G→A intergenic (+186/‑356) JCVSYN2_00665 → / → JCVSYN2_00670 tRNA N6‑adenosine(37)‑N6‑threonylcarbamoyltransferase complex dimerization subunit TsaB/tRNA‑Ser

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816165,2220GA98.9% 3786.3 / 24.1 1231intergenic (+186/‑356)JCVSYN2_00665/JCVSYN2_00670tRNA N6‑adenosine(37)‑N6‑threonylcarbamoyltransferase complex dimerization subunit TsaB/tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (777/435);  minor base . (2/12);  total (780/451)
Fisher's exact test for biased strand distribution p-value = 2.05e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1252 total aligned reads displayed.

CTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTAAAAGAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTA  >  CP016816/165097‑165347
                                                                                                                               |                                                                                                                                
 ttaaaTTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAAATTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaat                                                                                                                               <  2:1165419/125‑1 (MQ=255)
  ttAATTGTTTTACTTTACTAGTTGTTAATATTTAGTTTCATTAAATTTTATCACTTGTATGAATTAGTAAGTAAATAAAACTATTTTGTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaatt                                                                                                                              <  2:3836793/128‑1 (MQ=255)
    aaTTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaattaa                                                                                                                            >  2:884062/1‑128 (MQ=255)
     aTTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaattaat                                                                                                                           <  2:602029/128‑1 (MQ=255)
       tGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAAttt                                                                                                                         <  2:847504/128‑1 (MQ=255)
       tGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAAttt                                                                                                                         <  1:2235024/128‑1 (MQ=255)
       tGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAAttt                                                                                                                         <  1:3786608/128‑1 (MQ=255)
       tGTAATACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGGAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAAttt                                                                                                                         <  1:1343610/128‑1 (MQ=255)
        gTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTg                                                                                                                        >  2:2149956/1‑128 (MQ=255)
        gTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTg                                                                                                                        >  1:3722369/1‑128 (MQ=255)
         tATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGa                                                                                                                       >  1:392935/1‑128 (MQ=255)
          aTTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTATAAAATTAATTTGaa                                                                                                                      >  2:1381324/1‑128 (MQ=255)
            tactttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATTAAAATTTTTAAAAAATTAATTTGAACt                                                                                                                    >  1:2762818/1‑128 (MQ=255)
             actttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACtt                                                                                                                   >  1:2142416/1‑128 (MQ=255)
               tttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATTTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTa                                                                                                                >  2:2887636/1‑128 (MQ=255)
                ttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTa                                                                                                                >  2:3060500/1‑128 (MQ=255)
                  actAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaaa                                                                                                              >  2:2975969/1‑128 (MQ=255)
                    tAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAACCTATTATCTTTTTAGATATTTTTGTATATTCAAATTTTTAAAAAATTAATTTTAACTTGTaaaaaa                                                                                                           >  2:2837809/1‑128 (MQ=255)
                     aGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAAGTATTATCTTTTTAGATTTTTTTTGTTTATGAAAAGTTTTAAAAAATTAATTTGAAGTTGTaatata                                                                                                           >  2:2028977/1‑124 (MQ=255)
                     aGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATTCAAATTTTTAAAAAATTAATTTTAACTTGTaaaaaa                                                                                                           >  2:2510001/1‑128 (MQ=255)
                     aGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaaaaaa                                                                                                           >  2:404319/1‑128 (MQ=255)
                      gTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAAt                                                                                                          >  2:3311624/1‑128 (MQ=255)
                           taataTTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATTCAAATTTTTAAAAAATTAATATGAACATGAAAAAAATAGAtt                                                                                                    >  2:678111/1‑128 (MQ=255)
                           taataTTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtt                                                                                                    >  1:3073750/1‑128 (MQ=255)
                           taataTTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtt                                                                                                    >  1:3184361/1‑128 (MQ=255)
                             atatTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttt                                                                                                  >  1:341690/1‑128 (MQ=255)
                               atTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttt                                                                                                >  1:2075855/1‑128 (MQ=255)
                                tttAGTTTAATTAATTTATCGCAATTGTATTAATTAGTAATAAAAAAAAAATATTATCATTTTATATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTTAAATTGTAAAAAAAAGCttttttt                                                                                               <  1:3676530/128‑1 (MQ=255)
                                 ttAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTAGTATCTTTTTAGATATTATTTGTAGATGCAAATTTTTAAAAAAGTAATTTGAACTTGAAAAAAATAGCttttttgt                                                                                              >  2:3482609/1‑126 (MQ=255)
                                  tAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttt                                                                                             >  1:1592325/1‑128 (MQ=255)
                                    gTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttt                                                                                           >  2:1217216/1‑128 (MQ=255)
                                     tttCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttt                                                                                          >  1:2540965/1‑128 (MQ=255)
                                     tataaTTAATTTATCGGTATTGTAAGAATTAGTAAAAAACTAAAACTATTTACATTTTATATATTTTTTTTTAATGCAAATTTTTTAAAAATTAATTTGAACTTTTAAAAAATAGCtttttttttttt                                                                                          <  1:2607873/124‑1 (MQ=255)
                                         aTTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttttt                                                                                      >  1:3329247/1‑128 (MQ=255)
                                         aTTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttttt                                                                                      >  1:1677665/1‑128 (MQ=255)
                                              tttATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttttttttata                                                                                 >  1:3610254/1‑125 (MQ=255)
                                                 tcttcACTTTTATGAAAAACTAATAAAATAAAAAAATTATCTTTTTATATATTTTTTGTATATTCAAAATTTTAAAAAAATAAATTTAACATGTAAAAAAAAGCCTTTTTTTTTTTTTTTTTATAATg                                                                             <  1:3278782/124‑1 (MQ=255)
                                                   cGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTTTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATATCttattttttttttttttaaaatgtaa                                                                            >  2:2614725/1‑119 (MQ=255)
                                                   cGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttttttttttatatta                                                                            >  1:821132/1‑121 (MQ=255)
                                                   cGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttttttttatattgaa                                                                            >  2:1060989/1‑120 (MQ=255)
                                                   cGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTtataatttaa                                                                            >  2:1683478/1‑122 (MQ=255)
                                                     cACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATGaaat                                                                         >  1:2885606/1‑127 (MQ=255)
                                                      aatttaattaaTTATTAAGAAACTAAAAATAATATATTTTTATATATTTTTTTTATATTCAAATTTTTAAAAAATTAATTTTAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATGGAAat                                                                        <  1:774372/119‑1 (MQ=255)
                                                       cTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTATTAATGAAAata                                                                       >  1:115497/1‑128 (MQ=255)
                                                        ttGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATTGAAata                                                                       >  1:1430719/1‑128 (MQ=255)
                                                           tATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTTATAATTGAAAtata                                                                     >  1:3119148/1‑128 (MQ=255)
                                                             tgtaattttaataaAATAAAAAAATTATTTTTTTTTATATTTTTTTTATATTCAAAATTTTAAAAAAATAAATTTAAATTTTTAAAAATATCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAAtt                                                                 <  1:960227/120‑1 (MQ=255)
                                                               aaTTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATTAaaatttaaattta                                                               >  1:105655/1‑127 (MQ=255)
                                                                  tAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAATATAGCTTTTTTTTTTTTTTTTTTATAATTGAAATATAAATTTTTgg                                                            >  2:1029152/1‑128 (MQ=255)
                                                                  tAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTatg                                                            >  1:3847463/1‑126 (MQ=255)
                                                                   tgaaattaaataaaacttTTTTTTTATTATTTATTTTTTTTATATTGAAAATTTTTAAAAATTTAATTTTAATTTTTAAAAAATGCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGt                                                          <  2:2091303/118‑1 (MQ=255)
                                                                    ttaataaAATAAATATATTTTTTTTTTTTTTATTTTTTTTTTTTTAAAATTTTTAAAAAATTTATTTGAACTTGTAAAAAATATCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTa                                                         <  2:2212592/127‑1 (MQ=255)
                                                                    gTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATGTAAATTTAAATTTTAGTTa                                                         >  2:1314831/1‑128 (MQ=255)
                                                                          aactaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAAAGTAAATATAAATTTTATATAtttttt                                                   >  1:2479064/1‑126 (MQ=255)
                                                                           actaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTTTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATTTAAATATAAATTTAATTTATttttttt                                                 >  1:1753441/1‑127 (MQ=255)
                                                                           actaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTAAAATTTAAAATAAAATTTATGtttattttt                                                  >  2:1472562/1‑120 (MQ=255)
                                                                             taaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTAATTTATttttttt                                                 >  1:1819986/1‑127 (MQ=255)
                                                                             taaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTATATATTGTAAATATAAATTTTAGGTTTTTttttgt                                                >  2:3604702/1‑128 (MQ=255)
                                                                             taaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTATTTATtttttttta                                               >  1:2172610/1‑125 (MQ=255)
                                                                             taaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGATTTTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTATTTATtttttttt                                                >  1:1719267/1‑126 (MQ=255)
                                                                               aaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGTTATTTttttgta                                               >  1:61049/1‑127 (MQ=255)
                                                                               aaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATTAAAAAATAAATTTTAGTAATtttttttataa                                             >  2:269191/1‑123 (MQ=255)
                                                                               aaaattttatTTTTTTTTTTATTTTTTGTTTTTTTAAATTTTTAAAAAATTAATTTTAAATTTTAAAAAATATTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCt                                              <  1:10008/122‑1 (MQ=255)
                                                                                aactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGGAAAATTAATTTTTAGTtttttttatttataa                                            >  2:1936994/1‑119 (MQ=255)
                                                                                aaatattatATTTTTATCTTATTTTTGTATATTCAAATTTTTAAAAAATTTATTTGAACTTGTAAAAAATTTCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTa                                             <  1:3526166/125‑1 (MQ=255)
                                                                                  aaattatATTTTTTTTTATTTTTTGTATATTGAAATTTTTTAAAAATTTATTTGAACTTGTTAAAAATAGGTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAg                                           <  2:3446075/126‑1 (MQ=255)
                                                                                   tattatCTTTTTAGATATTTTTTGTATATGCAATTTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTTTAATGTTAATTTATTTTTTATTTTTTttgtttgctatg                                           >  2:2415646/1‑121 (MQ=255)
                                                                                   tattatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTAAAATGTAAAATAAAATTTTATGTTTTTTGTTTTATaatt                                          >  1:451382/1‑126 (MQ=255)
                                                                                     ttatTTTTTTTTATATTTTTTTTTATTTCAAATTTTTAAAAAATTAATTTTAAATTTTAAAAAATATCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTAt                                        <  1:125665/128‑1 (MQ=255)
                                                                                     ttatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTAATAATTAAAAAATAAATTTTATTTATTTTTTTGtatatttaaat                                      >  2:497070/1‑121 (MQ=255)
                                                                                      tttttttttAAATATTTTTTATATTTGAAAATTTTTAAAAAATAATTTTAAATTTTAAAAAAAATTGTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATc                                      <  1:1496088/124‑1 (MQ=255)
                                                                                       atCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTAAGTTATTTTTTTTTATAATtatata                                    >  1:2947005/1‑128 (MQ=255)
                                                                                        tCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTAAAATTTAAAAATAAAATTTATTAATTTTTTTGTATaataaatt                                      >  1:2015555/1‑122 (MQ=255)
                                                                                          ttttttttatttttttttATATTAAAATTTTTAAAAAATTAATTTTTAATTTTAAAAAATA‑‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTaaaa                                 <  2:3262987/118‑1 (MQ=255)
                                                                                           ttttAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATTGAAATATAAATTTTAGTTATTTTTTTGTAAAATTATATaaaa                                 >  1:3478788/1‑128 (MQ=255)
                                                                                              ttatttttttttttATATTAAAATTTTTAAAAAATAAATTTTAAATTTTAAAAAAA‑‑TTATTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATa                            <  1:3232076/122‑1 (MQ=255)
                                                                                              tatattttttttttATATTTAAATTTTTAAAAAATTAATTTTAAATTTTTAAAAATTTTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTaaaaaaa                              <  1:2368252/122‑1 (MQ=255)
                                                                                                ttattattttGTATATGTAAATTTTTAAAAAATTAATTTTAACTTTTAAAAAATTGTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAg                           <  2:3701541/122‑1 (MQ=255)
                                                                                                 attttttttttttATTTAAATTTTTAAAAATTTTATTTTATTTTTTAAAAAATTGATTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAg                           <  2:1576239/125‑1 (MQ=255)
                                                                                                 atatTTTTTTTATATTCAAATTTTTAAAAAATTAATTTTAACTTTTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAg                           <  2:942988/128‑1 (MQ=255)
                                                                                                      ttttGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATTGAATTATAAATTTTAGTTATTTTGTTTTATAATTATATAAAAAAATATaaat                       >  1:1866129/1‑127 (MQ=255)
                                                                                                      ttttGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGTTTTTTTTTTTTAAAATTTTATAAAAAAATAAAAAgt                      >  2:277408/1‑127 (MQ=255)
                                                                                                        ttttttttatttaaaTTTTTAAAAAATTAATTTTAAATTGTAAAAAATA‑‑TATTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGaa                    <  1:1217216/116‑1 (MQ=255)
                                                                                                        ttGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTTAATGTAAATTTAATTTTTAGTTTTTTTTTTTTATAATTATATAAAAAAATATaaataaa                    >  2:3767393/1‑124 (MQ=255)
                                                                                                        ttGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTATGTATTTTTTTTTATAATTTTATAAAAAAATATAAATGaa                    >  1:685039/1‑128 (MQ=255)
                                                                                                         tGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTT‑‑TAATTGTAATAAAAATTTTAGGTTATTTTTTGTTATAAGATAAAAAAAAATATAAATGtaaa                  >  2:3391836/1‑128 (MQ=255)
                                                                                                         tGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATTTAATTATAAATTTAATTTATTTTTTTTTATAATTTTATAAAAAATTATaaataaaa                   >  1:2662406/1‑123 (MQ=255)
                                                                                                          tttttatttaaaaTTTTTAAAAAATTAATTTTAAATTTTAAAAAAATGC‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGaaa                   <  2:1753441/118‑1 (MQ=255)
                                                                                                            atatGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATGGAAATAATAATTTTTGCTTTTTTTTTGTCTAATATAATAAAAAAATATCAAGGataaaa                >  2:756493/1‑128 (MQ=255)
                                                                                                               tatttaaaTTTTTAAAAAATTAATTTTAACTTGTAAAAAATATATTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAat               <  1:2324987/123‑1 (MQ=255)
                                                                                                                tGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTAAAATTTATATATAAATTTTATTAATTTTTTTTTATATTTATATAAAAAAATAAAAATAAAAAAatat             >  1:30183/1‑128 (MQ=255)
                                                                                                                 ttaaaaTTTTTAAAAATATAATTTTAAATTTTAAAAAATTGTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAatat             <  1:1964484/125‑1 (MQ=255)
                                                                                                                  cAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATTATTAAAATTATAATTTTTTGTATTTTGTTTTGATAATATTAAAAAAAATAAAAAAGaaaaaaatttt           >  2:3101243/1‑123 (MQ=255)
                                                                                                                    aaaTTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTTAATGTAAATAAAATTTTTAGGTTTTTTTTTTTATAATTATATAAAAAAATAAAAAGAAAAAattatttt          >  2:2308699/1‑126 (MQ=255)
                                                                                                                    aaaTTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATTAAAAAAAAAATTTTATTTTTTTTTTTGTATAATTATAAAAAAAAAAAAAAATTaaaaataatttt          >  2:318963/1‑121 (MQ=255)
                                                                                                                      aTTTTTTAAAAATTAATTTTTAATTTTAAAAAATTTATTTTTTTTTTTTTTTTTTATAATTGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTa        <  2:1867203/128‑1 (MQ=255)
                                                                                                                      aTTTTTAAAAAATTTATTTTTAATTTTAAAAAATATTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTa        <  1:873178/128‑1 (MQ=255)
                                                                                                                        tttAAAAAATTTATTTTTAATTTTTAAAAATT‑‑TTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTaaaa     <  2:3525709/128‑1 (MQ=255)
                                                                                                                          ttaaaaaaTTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATATTTTAAATATAAATTTTATGTTTTTTTTTTTATATTTATATAAAAAAATTAAAAGTAAAAAATTTTTTTaaattt   >  1:2841084/3‑125 (MQ=255)
                                                                                                                           taaaaaaTTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATATTTTAAATATAAATTTTAGTTATTTTGTTTTATAATTTTATAAAAAAATATAAAGTAAAAAATTTTTTTAAAAGt   >  1:509581/2‑128 (MQ=255)
                                                                                                                               |                                                                                                                                
CTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTAAAAGAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTA  >  CP016816/165097‑165347

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: