Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 165,218 A→T intergenic (+182/‑360) JCVSYN2_00665 → / → JCVSYN2_00670 tRNA N6‑adenosine(37)‑N6‑threonylcarbamoyltransferase complex dimerization subunit TsaB/tRNA‑Ser

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816165,2180AT97.8% 3632.5 / ‑2.6 1240intergenic (+182/‑360)JCVSYN2_00665/JCVSYN2_00670tRNA N6‑adenosine(37)‑N6‑threonylcarbamoyltransferase complex dimerization subunit TsaB/tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base A (4/23);  new base T (784/429);  total (788/452)
Fisher's exact test for biased strand distribution p-value = 1.95e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1285 total aligned reads displayed.

TTTACTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTAAAAGAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAG  >  CP016816/165093‑165345
                                                                                                                             |                                                                                                                                  
 ttACTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTtaaa                                                                                                                                 <  1:416259/128‑1 (MQ=255)
   aCTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTtaaaaa                                                                                                                               >  1:1754707/1‑126 (MQ=255)
   aCTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTtaaaaa                                                                                                                               <  2:3486739/128‑3 (MQ=255)
    cTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTaaaaaa                                                                                                                              >  1:2864632/1‑128 (MQ=255)
       tAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaatta                                                                                                                          <  1:3038236/128‑1 (MQ=255)
       tAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaatta                                                                                                                          <  1:3213168/128‑1 (MQ=255)
           tGTATTACTTTACTTTTTGATAATATTTAGTTTAATTAATTTATCTCAAATGTATGAATTAGTAAGATACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAAttt                                                                                                                      <  1:2553497/128‑1 (MQ=255)
           tGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAAttt                                                                                                                      >  2:1790458/1‑128 (MQ=255)
           tGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAAttt                                                                                                                      <  1:3259930/128‑1 (MQ=255)
            gTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTATAGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGa                                                                                                                    <  2:1962142/128‑1 (MQ=255)
             tATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCAATTGTATGAATTAGTAAGTAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGa                                                                                                                    <  1:2501831/128‑1 (MQ=255)
                 actttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTTAACtt                                                                                                                >  1:135126/1‑128 (MQ=255)
                   tttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGt                                                                                                              >  2:125461/1‑128 (MQ=255)
                   tttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGt                                                                                                              >  1:2673530/1‑128 (MQ=255)
                    ttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTa                                                                                                             <  2:2313994/128‑1 (MQ=255)
                    ttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTa                                                                                                             >  1:3749323/1‑128 (MQ=255)
                    ttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATACAAATTATTAAAAAATTAATTTTAACTTGTa                                                                                                             >  2:269584/1‑128 (MQ=255)
                     tactTGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaa                                                                                                            >  2:2999465/1‑128 (MQ=255)
                     tactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaa                                                                                                            <  2:1888302/128‑1 (MQ=255)
                      actAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaaa                                                                                                           >  2:1175328/1‑128 (MQ=255)
                      actAGTTGATAATATTTAGTTTAATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaaa                                                                                                           <  2:3604805/128‑1 (MQ=255)
                      actAGTGGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaaa                                                                                                           >  2:3265715/1‑128 (MQ=255)
                          gTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAAt                                                                                                       >  1:1867203/1‑128 (MQ=255)
                           ttGATAATATTTTGTTTCATTAATTTATAGCAATTTTATTAATTAGTAAGAAAAAAAAAATATTATATTTTTATATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTTAACTTGTAAAAAATa                                                                                                      <  2:1034157/128‑1 (MQ=255)
                            ttataataTTTAGTTTAATTAATTTATCGCACTTGTATGAATTAGTAATAAAATAAAACTATTATCTTTTTTTATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAg                                                                                                    <  2:1352062/126‑1 (MQ=255)
                               taataTTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTTAACTTGTAAAAAATATCtt                                                                                                 >  2:849094/1‑128 (MQ=255)
                                  tatTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttt                                                                                              >  1:527964/1‑128 (MQ=255)
                                    tttAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATATCatttttt                                                                                            >  1:1496596/1‑128 (MQ=255)
                                      tAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttt                                                                                         >  2:2784125/1‑128 (MQ=255)
                                      tAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttt                                                                                         >  1:3101664/1‑128 (MQ=255)
                                      tAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttt                                                                                         >  1:1592325/1‑128 (MQ=255)
                                         tttCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATATCtttttttttttt                                                                                      >  1:849874/1‑128 (MQ=255)
                                         tttCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttattttt                                                                                      >  2:3122144/1‑128 (MQ=255)
                                          ttaaTTAATTTATTGACATTTTATTAATTTTTAAAAAACTAAAAATATTATATTTTTAGATTTTTTTTGTATATTCAAATTTTTTAAAAATTAATTTTAAATTGTAAAAAATAGCttttttttttttt                                                                                     <  2:2851025/125‑1 (MQ=255)
                                          ttCATTAATTTTACTCACTTGTATTAATTACTAATAAAATAAAACTATTATCTTTTTATATATTTTTTGTATTTGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttt                                                                                     <  2:371582/128‑1 (MQ=255)
                                            cATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATTCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATATCtttttattttttttt                                                                                   >  1:64334/1‑128 (MQ=255)
                                            cATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttttt                                                                                   >  1:3448763/1‑128 (MQ=255)
                                             aTTAATTTATCGCACTTGTTTGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttttt                                                                                  >  2:1519207/1‑128 (MQ=255)
                                             aTTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttttt                                                                                  >  1:1677665/1‑128 (MQ=255)
                                              ttAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttttttt                                                                                 >  1:2179805/1‑128 (MQ=255)
                                                  tttATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTtat                                                                             >  2:42758/1‑128 (MQ=255)
                                                  tttATAGCAATTGTTTTAATTATTAAGAAAATAAAAATATTATATTTTTATATATTTTTTGTATATTCAAATTTTTAAAAAATTAATTTTAAATTTTAAAAAATAGCttttttttttttttttttata                                                                             <  2:1105166/128‑4 (MQ=255)
                                                   ttATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTTAACTTGTAAAAAATATCttttttttttttttttttttaa                                                                            >  2:2899476/1‑125 (MQ=255)
                                                     atttcACTTTTTTTAATTATTAATAAAATAAAAATATTATATTTTTTTATATTTTTTTTATATTCAAATTTTTAAAAAATTAATTTTTAATTTTTAAAAATATCTTTTTTTTTTTTTTTTtataatgg                                                                          <  2:604920/124‑5 (MQ=255)
                                                     aTCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTTAATg                                                                         >  1:698023/1‑128 (MQ=255)
                                                      attcaattttttGATTTAGTAATAAAATAAAAATTTTATTTTTTTTTATATTTTTTTTTTTTTGAAATTTTTTAAAAAATAATTTTAAATTGTAAAAAATATCTTTTTTTTTTTTTTTTTTATAATgg                                                                        <  2:3397928/117‑1 (MQ=255)
                                                        gCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttttttaaaaatggaaa                                                                       >  1:3499320/1‑117 (MQ=255)
                                                           cTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAATAAATAAATTTGAAGTTGTTAAAAATTTATTTTTTTTTGTTTATTTTaatattaatatt                                                                    >  2:678248/1‑120 (MQ=255)
                                                            ttGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATTAATTTAAata                                                                   >  1:3395022/1‑128 (MQ=255)
                                                             tGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTGTTTTTTTTTTTATAATGTAAATTTaa                                                                >  2:2534804/1‑128 (MQ=255)
                                                              gTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTATTTTATAATGTAAATATaa                                                                >  2:2119481/1‑128 (MQ=255)
                                                              gTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATaaa                                                               >  2:1635774/1‑128 (MQ=255)
                                                               taattaTTAATAATAAAATAAATATTTTTTATTTTTTTTTATTTTTTGTATATGAAATTTTTTAAAAAAATAATTTTAAATTTTTAAATATAG‑‑ATTTTTTTTTTTTTTTTTATAATGTAAATATAAAt                                                              <  1:1439399/123‑1 (MQ=255)
                                                                aTGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATTTAAATATAAAt                                                              >  1:47958/1‑128 (MQ=255)
                                                                aTGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTATTTTTTTTTTATAATTAAAATAAAAAtt                                                             >  1:790434/1‑128 (MQ=255)
                                                                  gAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTAAATTTAAATATAAAttt                                                            >  1:3816643/1‑128 (MQ=255)
                                                                  gAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATTTAAATATAAAttt                                                            >  2:3194415/1‑128 (MQ=255)
                                                                  gAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATTTAAATTTAAAtttt                                                           >  1:2083117/1‑128 (MQ=255)
                                                                   aaTTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATTAaaatttaaattta                                                           >  1:105655/1‑127 (MQ=255)
                                                                      tatttataaaataaaaattTTATTTTTTTATATATTTTTTTTATATTCAAATTTTTAAAAAATTAATTTTTAATTTTAAAAAATA‑‑TGTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGt                                                       <  2:1708997/121‑1 (MQ=255)
                                                                      tattaataaAATAAAACTATTATCTTTTTATTTATTTTTTTTATATTATAATTTTTAAAAAATTAATTTTAAATTTTTAAAAATATCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAgg                                                        <  1:98467/125‑1 (MQ=255)
                                                                      tAGTAAGAAACTAAAAAATTTATTATATTAGATATTTTTTTTTTATGAAAATTTTTAAAAAATTAATTTGAACTTTTAAAAAAAATCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAgg                                                        <  1:2021837/128‑1 (MQ=255)
                                                                       atttattaaataaaaataTTATTTTTTTTTTTTTTTTTTTTATTTTCAAATTTTTTAAAAATTTATTTTAAATTTTAAAAAATTTCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGt                                                       <  1:113689/118‑1 (MQ=255)
                                                                       aGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATTGAAATATAAATTTTAgtt                                                       >  1:893985/1‑126 (MQ=255)
                                                                       aGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTATGTAt                                                     >  2:3787096/1‑128 (MQ=255)
                                                                         ttattaaataaaaataTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAATTTTTAAAAAATTAAATTTTAATTTTTAAAAAAATCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTa                                                      <  1:2975969/120‑1 (MQ=255)
                                                                         taataaataaaaattaTTATTTTTTTTTTTATTTTTTTTATATTTAAATTTTTAAAAAATTAATTTTTAATTTTTAAAAATATCTTTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTAGGTAt                                                     <  1:2330574/119‑1 (MQ=255)
                                                                           ataaaataaaaataTTTTATTTTTATATTTTTTTTTTATATTCAAATTTTTAAAAAAATAATTTTAAATTTTAAAAAATATCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTAttt                                                   <  1:3311624/126‑1 (MQ=255)
                                                                             aaactaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATTTAAATATAAAtttttttttttttt                                                 >  1:3867995/1‑118 (MQ=255)
                                                                                ctaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTT‑‑TAATGGAAATATAAATTTTATTTATTtttttga                                             >  2:3314284/1‑127 (MQ=255)
                                                                                 taaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTAATTTATttttttt                                              >  1:1819986/1‑127 (MQ=255)
                                                                                 taaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGATTTTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTATTTATtttttttt                                             >  1:1719267/1‑126 (MQ=255)
                                                                                 taaaactaTTATCTTATTAGAAATTTTTTGTATATGCAATTTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGATTTTTTTTATTTTTTTTAAAAAATTAAATATAATTTTTATGTATAttttttg                                             >  2:2450085/1‑126 (MQ=255)
                                                                                   aaaattttatTTTTTTTTTTATTTTTTGTTTTTTTAAATTTTTAAAAAATTAATTTTAAATTTTAAAAAATATTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCt                                           <  1:10008/122‑1 (MQ=255)
                                                                                    aaatattatATTTTTTTATATTTTTTGTATATTAAAATTTTTAAAAAATTAATTTTAACTTGTAAAAAATTGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAg                                        <  2:3062629/125‑1 (MQ=255)
                                                                                        attttattttttTATATTTTTTTTTTTTTAAAATTTTTAAAAAATTAATTTTTAATTGTAAAAAATA‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTAt                                     <  1:3591744/122‑1 (MQ=255)
                                                                                        attatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTATTTATTTTGTTTTATAATTa                                      >  1:2394189/1‑128 (MQ=255)
                                                                                         ttttttttttAGATATTTTTTTTATAATCAAAATTTTAAAAAATTAATTTGAACTTGTAAAAAATA‑‑‑TCTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCt                                   <  2:203990/123‑1 (MQ=255)
                                                                                         ttttatttttATATTTTTTTTTTATATTTAAAATTTTTAAAAATTAATTTTTAATTTTAAAAAATATTTTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGt                                       <  2:2166458/123‑1 (MQ=255)
                                                                                         ttatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATATTTTAAATAAAAATTTTATTTTTTTTGTTGTATaattt                                      >  2:3451606/1‑125 (MQ=255)
                                                                                         ttatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTATTTTTTTTTTAAATTTAAAAAAAAATTTTTATGAATTTttttgtaatatat                                      >  1:3632057/1‑121 (MQ=255)
                                                                                         ttaatttttttGTTATTTTTTTTATATTCAAATTTTTAAAAAATTAAATTTAAATTTTTAAAAAAT‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATc                                    <  1:2910393/123‑1 (MQ=255)
                                                                                             cTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTAAAATTAAAATAAAAATTTTAGTTATTTTTTTTTATAATTAtaaaaa                                >  2:522546/1‑123 (MQ=255)
                                                                                              tttttAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATATAAATTAAAAATTAAATTATTTTTTTTTATaatttattaa                                 >  1:261946/1‑120 (MQ=255)
                                                                                               ttttAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTTTTTTCTAAGTATCTaaa                                >  1:3148626/1‑128 (MQ=255)
                                                                                                 ttAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTTAATGTAAAAATAAATTTTTGGTATTTTGTTGTCTAATTaaaaaaaaaa                             >  2:3843660/1‑128 (MQ=255)
                                                                                                 ttAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATTGAAATATAAATTTTATGTATTTTAATTTATAAGTATAaaaaaaaa                            >  1:2144396/1‑128 (MQ=255)
                                                                                                    tattttttttttttATTTAAATTTTTAAAAAATTAATTTTTATTTTTTAAAATTTTCTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAg                         <  2:1405080/124‑1 (MQ=255)
                                                                                                     atatTTTTTTTATATTCAAATTTTTAAAAAATTAATTTTAACTTTTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAg                         <  2:942988/128‑1 (MQ=255)
                                                                                                     atatTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATTGAAATAAAAATTTTAGGTATTTTTTTGTGAAATTATATAAAAAAATat                         >  2:2222576/1‑127 (MQ=255)
                                                                                                         tttttGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTTAATGGAAATTATAATTTTAGGTTTTTTTTTTTATAATTTTATAAAAAAATaatgaa                     >  2:262715/1‑123 (MQ=255)
                                                                                                           tttGTATATGCAAATTTTTAAAAAATTAATTTTAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTAAAATTAAAATATAATTTTTTGTAATTTTTTTTGATAAATATAAATAAAAATATAAAga                    >  2:884357/1‑127 (MQ=255)
                                                                                                            ttttattttgaaaTTTTTAAAAAATTAATTTTAACATTTAAAAAATATATTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGaa                  <  1:224512/118‑1 (MQ=255)
                                                                                                            ttttataTTTAAATTTTTAAAAAATTAATTTTTAATTTTAAAAAATATATTTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGaa                  <  1:2784125/125‑1 (MQ=255)
                                                                                                             tGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTTAATGTAAATATAAATTTTAGTTATTTTTTTTTATAATTTTATAAAAAAATATaaataaaa                 >  1:1260008/1‑123 (MQ=255)
                                                                                                             tGTAAATTCAAATTTTTAAAAAAATAATTTTAAATTGTAAAAAAATGATTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGaaa                 <  1:3700980/128‑1 (MQ=255)
                                                                                                               tataTGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTCTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGaaa                 <  1:1235090/128‑1 (MQ=255)
                                                                                                                   ttaaaaTTTTTAAAAATATAATTTTAAATTTTAAAAAATTGTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAatat           <  1:1964484/125‑1 (MQ=255)
                                                                                                                     cAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTGTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTTGTTTTTTTTTTTTCTAATTTTCTAAAAAAAAATAATGGAAAAAATATTg         >  2:775682/1‑128 (MQ=255)
                                                                                                                          tttAAAAAATTTATTTTTAATTTTTAAAAATTTTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTaaaa   <  2:3525709/128‑1 (MQ=255)
                                                                                                                             |                                                                                                                                  
TTTACTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTAAAAGAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAG  >  CP016816/165093‑165345

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: