Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 484,791 T→A intergenic (‑100/+71) JCVSYN2_02220 ← / ← JCVSYN2_02225 hypothetical protein/50S ribosomal protein L7/L12

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816484,7910TA98.2% 5405.0 / 22.0 1813intergenic (‑100/+71)JCVSYN2_02220/JCVSYN2_02225hypothetical protein/50S ribosomal protein L7/L12
Reads supporting (aligned to +/- strand):  ref base T (19/14);  new base A (923/857);  total (942/871)
Fisher's exact test for biased strand distribution p-value = 5.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1875 total aligned reads displayed.

ATTTAAAAAATAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTTAAAAAAAAAACAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATTTCT  >  CP016816/484668‑484913
                                                                                                                           |                                                                                                                           
   tAAAAAATAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCATAAGCTCTTATCAACTAAACTACAATCGATTATTTAATTATATTTTTTTTTAaaataaa                                                                                                                     >  2:692035/1‑128 (MQ=255)
       aaaTAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCTATTATTTAATTATATTTTTTTTTAatataaaaagc                                                                                                                 >  1:1055110/1‑126 (MQ=255)
       aaaTAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAatataaaaagc                                                                                                                 >  2:1006377/1‑126 (MQ=255)
         aTAGTCAAAACTCATAGATTTAATCATTTTATAGTTGACCAAAAAATAAGATAGATCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCaa                                                                                                               <  1:300427/128‑1 (MQ=255)
          tAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAATCTCTTAACAACTAAACTACACTCTATTATTTAATTATATTTTTTTTTAATATAAAAAGCAAt                                                                                                              >  1:1428962/1‑128 (MQ=255)
            gtgaataatCATAGATTTAATCCTTTTATAGTTGTAAAAAAAATAAGATAGAGAATAGCAGAATCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTa                                                                                                            <  2:1763859/120‑1 (MQ=255)
              cAAAACTCATATCATAAATCCATAAAAAGATGACCAAAAAATAAGATATAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTaaa                                                                                                          <  2:3067754/128‑1 (MQ=255)
              cAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTaaa                                                                                                          <  2:3472916/128‑1 (MQ=255)
              cAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTaaa                                                                                                          >  1:2942008/1‑128 (MQ=255)
              cAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTaaa                                                                                                          <  2:1291015/128‑1 (MQ=255)
               aaaaCTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAac                                                                                                         <  1:100194/128‑1 (MQ=255)
                aaaCTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAATCAATTAAaca                                                                                                        >  2:354900/1‑128 (MQ=255)
                aaaCTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATAATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAaca                                                                                                        >  1:700120/1‑128 (MQ=255)
                 aaCTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCATTTAAacac                                                                                                       >  2:1148619/1‑128 (MQ=255)
                 aaCTCATAGATTTAATACTTTTATAGTTGAACAAAAAATAAGATAGATCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAacac                                                                                                       <  1:2788574/128‑1 (MQ=255)
                  aCTCATAGATCTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAacaca                                                                                                      >  2:1362210/1‑128 (MQ=255)
                   cTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAacacac                                                                                                     >  2:3249375/1‑128 (MQ=255)
                    tCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAAcacaca                                                                                                    >  1:2270850/1‑128 (MQ=255)
                        aGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACaaaaa                                                                                                <  1:2510036/128‑1 (MQ=255)
                          aTTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCACTTAAACACACAAAAACt                                                                                              >  1:1500167/1‑128 (MQ=255)
                           tttAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAAcac                                                                                             >  2:2895633/1‑126 (MQ=255)
                           tttAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTc                                                                                             >  1:3719142/1‑128 (MQ=255)
                            ttAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGAATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCa                                                                                            <  1:338818/128‑1 (MQ=255)
                             tAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACAAACAAAAACTCAc                                                                                           >  1:2570703/1‑128 (MQ=255)
                             tAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACGCGATTATTTAAATATATTTTTGTTAATATAAAAAGCAATTAAACACACAAAAACTCAca                                                                                          >  2:2648031/1‑127 (MQ=255)
                             tAATCATTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAATCTCTTAACAACTAAAATAAACTCGATAATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAAAACACAAAAACTAAc                                                                                           >  2:2436991/1‑128 (MQ=255)
                             tAATCATTTTATAGTTGAACAAAAAATAAGCTAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAc                                                                                           <  2:1970348/128‑1 (MQ=255)
                               aTCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAccc                                                                                         >  1:3188631/1‑128 (MQ=255)
                                tCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAACCACACAAAAACTAACCCa                                                                                        >  2:3630330/1‑128 (MQ=255)
                                 ccTTTTATAGGTGACCAAAACATAAGATAGAGCATAGCAGAAGCGCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAccata                                                                                       <  1:1981818/128‑4 (MQ=255)
                                  cTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCAtt                                                                                      <  2:1627649/128‑1 (MQ=255)
                                   ttttATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAccataat                                                                                     <  2:2384694/128‑6 (MQ=255)
                                    tttATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTAt                                                                                    >  2:273098/1‑128 (MQ=255)
                                     ttATAGTTGACCAAAAAATAAGATAGCGCATAGCAGAAGCTCTTCACCACTAAACTACACTCGAATATGTAATTATATTTTTTTTTAATATAAAAAGCAATTTCACCCACAAAACCACACCCAATATg                                                                                   >  2:2538947/1‑128 (MQ=255)
                                     ttATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATg                                                                                   >  1:1594788/1‑128 (MQ=255)
                                     ttATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTATAATATAAAAATCAATTAAACACACAAAAACTCACCCATAAtt                                                                                   >  1:2635117/1‑127 (MQ=255)
                                     ttATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTAATTAATTATATTTTTTTTAATATAAAAAGCAATTAAACACTCAAAAACTACCCCACTAttg                                                                                  >  1:944904/1‑126 (MQ=255)
                                      tataGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATgg                                                                                  <  1:2122420/128‑1 (MQ=255)
                                       ataGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCAtataggt                                                                                 >  2:476750/1‑122 (MQ=255)
                                        taGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCTATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTg                                                                                <  2:2809422/128‑1 (MQ=255)
                                         aGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTGGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGa                                                                               >  1:2402169/1‑128 (MQ=255)
                                         aGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAccataatgggtga                                                                               <  2:3862614/128‑12 (MQ=255)
                                               ccgatatatactatatAGAATAGAAGAAGCTATTAACAACTAAAATACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTaa                                                                         <  2:3400895/116‑1 (MQ=255)
                                                 aaaaaaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTaaaa                                                                       <  1:3859085/128‑1 (MQ=255)
                                                 aaaaaaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTaaaa                                                                       >  2:2214898/1‑128 (MQ=255)
                                                  aaaaaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTaaaaa                                                                      <  1:126330/128‑1 (MQ=255)
                                                   aaaaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAat                                                                     >  1:3304848/1‑128 (MQ=255)
                                                     aaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAatta                                                                   >  2:2285254/1‑128 (MQ=255)
                                                        aaGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTc                                                                >  1:3796561/1‑128 (MQ=255)
                                                         aGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAac                                                                                                         >  1:1171016/1‑86 (MQ=255)
                                                         aGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCAtt                                                                                      >  1:2454273/1‑105 (MQ=255)
                                                         aGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACACATTATGGTGAGTTTAAAAattattaa                                                               >  2:3729659/1‑126 (MQ=255)
                                                         aGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCaa                                                              <  1:57911/128‑1 (MQ=255)
                                                            tAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCaaaa                                                            >  2:805349/1‑128 (MQ=255)
                                                            tAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAAATATTCaaaa                                                            >  2:1455608/1‑128 (MQ=255)
                                                             agagCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCaaaaa                                                           <  2:1909594/128‑1 (MQ=255)
                                                             agagCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCaaaaa                                                           <  1:761286/128‑1 (MQ=255)
                                                              gatcATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAAt                                                          <  1:205675/125‑1 (MQ=255)
                                                              gagCATAGCATAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAAt                                                          >  2:1229348/1‑128 (MQ=255)
                                                              gagCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAAt                                                          >  1:1675634/1‑128 (MQ=255)
                                                               agCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAAtt                                                         >  1:433196/1‑128 (MQ=255)
                                                                gCATAGCAGAAGCTCTTAACAACTAACCAACACTCGATTATTTAATAATATTTTTTTTTAATATAAAACTCAATTAACCACACAAAAACTCACCCATTAAGGTGAGTTTAAAAAATATTCAAACAttt                                                        >  2:866166/1‑128 (MQ=255)
                                                                     gCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGatta                                                   >  2:927161/1‑128 (MQ=255)
                                                                       agtaGCTCTTTACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAATACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGattatt                                                 <  1:1754581/125‑1 (MQ=255)
                                                                       aGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTATTAATATAAAAAGCAATTAAACACACAAAAACTCACACATTATATTGAATATAAAAATAAATCAAAAATATTataatt                                                 >  1:504731/1‑128 (MQ=255)
                                                                       aGAAGCTATTAACAAATAAACTACACTCGATTAATTAATTATATATTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGattatt                                                 <  1:1836377/128‑1 (MQ=255)
                                                                         aaGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTAtttt                                               >  1:88766/1‑128 (MQ=255)
                                                                            ctctTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTaaa                                            >  1:2705446/1‑128 (MQ=255)
                                                                             tctTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTAATATAAAAAGCAATTAGACACACAGAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATc                                          <  1:1338226/128‑1 (MQ=255)
                                                                               ttAACTACTATACTACTCTCGATTTTTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAGCCATTATTGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCa                                         <  2:108785/128‑1 (MQ=255)
                                                                               ttAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCa                                         <  2:2157380/128‑1 (MQ=255)
                                                                                tAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGATTTTAAAAATAATTCAAAAATTTGATTATTTTAAATCaa                                        >  2:2474715/1‑128 (MQ=255)
                                                                                 aacaacTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAAt                                       <  1:2185865/128‑1 (MQ=255)
                                                                                   caacTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTg                                     >  1:3521627/1‑128 (MQ=255)
                                                                                    aactaaactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGa                                    >  2:1545822/1‑128 (MQ=255)
                                                                                     actaaactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGaa                                   <  2:3321298/128‑1 (MQ=255)
                                                                                     aataaactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGaa                                   <  2:1711008/126‑1 (MQ=255)
                                                                                        aaactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCt                                <  1:3458446/128‑1 (MQ=255)
                                                                                           ctaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCa                             >  1:3662936/1‑128 (MQ=255)
                                                                                           ctaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCa                             >  2:1821312/1‑128 (MQ=255)
                                                                                               acTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCtt                         >  2:54536/1‑128 (MQ=255)
                                                                                                  cGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCaa                      <  1:3591330/128‑1 (MQ=255)
                                                                                                  cGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCaa                      >  2:2568915/1‑128 (MQ=255)
                                                                                                   gATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGc                           >  2:3181403/1‑122 (MQ=255)
                                                                                                    attattTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATTGTGAGTTTAAAAATTATTCAAAAAATTTATTATTTTAAATCAATTGAAGCTCCAGCTTCAACt                    >  1:1917217/1‑128 (MQ=255)
                                                                                                     ttattTAATTATATTTTTTTTTTAATATAAAAAGAAATTAAACACACAAAAACTCACACATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACt                    >  1:2858622/1‑128 (MQ=255)
                                                                                                      tattTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTaa                  <  1:83025/128‑1 (MQ=255)
                                                                                                      tattTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTaa                  <  2:62918/128‑1 (MQ=255)
                                                                                                         ttAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAAttt               <  1:3138855/128‑1 (MQ=255)
                                                                                                           aaTTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCGCTAGCTTCAACTAATTTAg             <  1:2350750/128‑1 (MQ=255)
                                                                                                            aTTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTGGAGCTTCAATTGATTTaaa            <  1:324211/128‑3 (MQ=255)
                                                                                                            aTTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGc            <  2:3790883/128‑1 (MQ=255)
                                                                                                            aTTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGc            <  1:11136/128‑1 (MQ=255)
                                                                                                             ttATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCAATTATGTTGATTTAAAAAATTATTAAAAAATTTGATTATATTAAATCAATTTAATCTCCATCTTCAACTAATTTacat           >  2:298184/1‑125 (MQ=255)
                                                                                                              tataTTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCtt          >  1:642515/1‑128 (MQ=255)
                                                                                                               atatTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCttt         >  2:2122051/1‑128 (MQ=255)
                                                                                                               atatTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAAATCCAGCTTCAACTAAGTTACCttt         >  2:2040168/1‑128 (MQ=255)
                                                                                                                tatTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTc        <  1:3052612/128‑1 (MQ=255)
                                                                                                                  tttttttttaATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAAttt               >  2:761400/1‑119 (MQ=255)
                                                                                                                      tttttaATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATttct  >  2:409221/1‑128 (MQ=255)
                                                                                                                           |                                                                                                                           
ATTTAAAAAATAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTTAAAAAAAAAACAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATTTCT  >  CP016816/484668‑484913

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: