Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 478,738 C→A G301V (GGT→GTT)  JCVSYN2_02210 ← DNA‑directed RNA polymerase subunit beta'

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816478,7380CA99.3% 4899.8 / NA 1672G301V (GGT→GTT) JCVSYN2_02210DNA‑directed RNA polymerase subunit beta'
Reads supporting (aligned to +/- strand):  ref base C (6/4);  major base A (868/781);  minor base T (5/7);  total (880/792)
Fisher's exact test for biased strand distribution p-value = 5.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1714 total aligned reads displayed.

GTAACATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTACCACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTCTAAACGTTTTAATAA  >  CP016816/478613‑478862
                                                                                                                             |                                                                                                                            
gTAACATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTaaca                                                                                                                            >  1:2127968/1‑128 (MQ=255)
   taaTACGTATTTCTTTTTTTTCAATTATTGATGTTGCACCGATTTCTTTAACTTTATTTAATCTATCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAtcacca                                                                                                                         <  2:3614760/126‑1 (MQ=255)
   aCATACGTTTTTCATTATTTACAATAATTGCTGGTGCACCCATTTCGTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCCAacacca                                                                                                                         >  2:2448027/1‑128 (MQ=255)
    cATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCAt                                                                                                                        <  2:615440/128‑1 (MQ=255)
        cGTTTTTCATTATTTACAATAATTGATTGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTaa                                                                                                                    <  2:2770626/128‑1 (MQ=255)
          tttttCATTATTTACAATAATTGATGGTTCAACGATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAAtt                                                                                                                  <  2:1660911/128‑1 (MQ=255)
          tttttCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAAtt                                                                                                                  >  1:1775774/1‑128 (MQ=255)
            tttCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGa                                                                                                                <  1:2425501/128‑1 (MQ=255)
              tCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTTaat                                                                                                              >  1:1227247/1‑128 (MQ=255)
              tCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAAC‑TTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGaata                                                                                                             >  1:143421/1‑128 (MQ=255)
               cATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGaata                                                                                                             >  1:860437/1‑128 (MQ=255)
               cATTATTTACAATAATTGATGGTGCACACATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGaata                                                                                                             <  2:2870870/128‑1 (MQ=255)
                attattTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGaataa                                                                                                            <  2:3474238/128‑1 (MQ=255)
                attattTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGaataa                                                                                                            <  1:172180/128‑1 (MQ=255)
                attattTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTATTCTATATAAATCATTAATTTCACAAGTAGTATATCTACAACCTTCATATTGaataa                                                                                                            >  2:2502721/1‑128 (MQ=255)
                 ttattTACATTAATTGATGGTTCACCCTTTTCTTAAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGaataat                                                                                                           <  1:1944527/128‑1 (MQ=255)
                 ttattTACAATAATTGATGGTGCAACCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGACGTAGTAAATCTAACACCATCTAATTGaataat                                                                                                           <  2:3708717/128‑1 (MQ=255)
                  tattTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAAtt                                                                                                          <  1:2628190/128‑1 (MQ=255)
                   attTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTg                                                                                                         <  1:995965/128‑1 (MQ=255)
                   attTACAATAATGGATGGTGCACACATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTg                                                                                                         <  1:2183462/128‑1 (MQ=255)
                    tttACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATATTTCATTTATAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTATTAAATCTAACACAATCTAATTTAATAAtttg                                                                                                        >  1:1151034/1‑126 (MQ=255)
                     ttACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGa                                                                                                       >  1:1354378/1‑128 (MQ=255)
                      tACAATAATTGATGGTGCAGCAATTTCTTTAACTGTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGAc                                                                                                      <  2:1499638/128‑1 (MQ=255)
                       aCAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACg                                                                                                     >  2:2133195/1‑128 (MQ=255)
                        cAATACATGATGGTGCAGCCAGTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAAGGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGa                                                                                                    <  1:2260012/128‑1 (MQ=255)
                           taatTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATg                                                                                                 >  1:1996079/1‑128 (MQ=255)
                            aatTGATGGTGAATTCATTTCTTTAACTTTTTTTAATATTTCTTTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGt                                                                                                <  2:83957/128‑1 (MQ=255)
                              ttgattgaGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCt                                                                                              <  1:1830068/128‑1 (MQ=255)
                              ttGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCt                                                                                              >  1:2540475/1‑128 (MQ=255)
                               tGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCtg                                                                                             >  1:2480915/1‑128 (MQ=255)
                                gATGGTGCACACATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCtgg                                                                                            <  2:1146366/128‑1 (MQ=255)
                                  tGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCtggtg                                                                                          >  1:432686/1‑128 (MQ=255)
                                    gTGCACCCTTTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGa                                                                                        <  1:372596/128‑1 (MQ=255)
                                    gTGCACCCATTTCTTAAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGa                                                                                        <  1:207235/128‑1 (MQ=255)
                                     tgaacacATTTGTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACAATCTAATTGAATAATTGGACGAATGTCTGGTGGaa                                                                                       <  2:3195263/122‑1 (MQ=255)
                                       cACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATa                                                                                     <  2:2288563/128‑1 (MQ=255)
                                       cACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATa                                                                                     >  2:1225976/1‑128 (MQ=255)
                                       cACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATa                                                                                     >  1:3452797/1‑128 (MQ=255)
                                       cACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATa                                                                                     >  2:292711/1‑128 (MQ=255)
                                       cACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATAATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAAATGGACGAATCTCTGGTGTAATa                                                                                     >  2:2346773/1‑128 (MQ=255)
                                         cactattatttAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAAGACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAAc                                                                                   <  2:682602/120‑1 (MQ=255)
                                           cATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACAATCTAATTGATTAATTGGACGAATGTCTGGTGCAATAACtt                                                                                 >  1:735220/1‑127 (MQ=255)
                                              ttCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGaa                                                                              <  1:3174048/128‑1 (MQ=255)
                                               tatttAAATTTTTTTAATCTTTCATTTCTAATAATGATTATTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATATTTGGACGAATGTCTGGTGGAATAACTGGAAt                                                                             <  2:2016824/126‑1 (MQ=255)
                                                  ttAACTTTTTTTAATCTATCAATTCTTATATTGATTATTATATATAAATCATTAATTTCAGAAGTAGTAAATCTAAAACGATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTAc                                                                          <  2:3549175/128‑1 (MQ=255)
                                                       tttttttAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCa                                                                     <  1:1270962/128‑1 (MQ=255)
                                                       tttttttAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCa                                                                     >  2:2199970/1‑128 (MQ=255)
                                                       tttttttAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCa                                                                     <  1:3327926/128‑1 (MQ=255)
                                                          ttttAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATaa                                                                  <  2:432189/128‑1 (MQ=255)
                                                               aTCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACcatt                                                             <  2:3787252/128‑1 (MQ=255)
                                                                tCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACcattc                                                            <  1:27781/128‑1 (MQ=255)
                                                                  tttCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACcattcat                                                          <  2:1386411/128‑1 (MQ=255)
                                                                     cATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCAttcattca                                                       >  1:603859/1‑128 (MQ=255)
                                                                      atatCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAg                                                      <  1:2709065/125‑1 (MQ=255)
                                                                      aTTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGTACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAAACATTCATTCAg                                                      >  1:3416983/1‑128 (MQ=255)
                                                                        ttCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGa                                                    >  2:2081644/1‑128 (MQ=255)
                                                                         tCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGTACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGAc                                                   >  1:2861014/1‑128 (MQ=255)
                                                                          cTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGTATTACTCTCATAACCATTCATTAAGGCCg                                                  >  2:1935621/1‑128 (MQ=255)
                                                                          cTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACg                                                  >  2:3266801/1‑128 (MQ=255)
                                                                            aataatGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGtt                                                >  2:74961/1‑128 (MQ=255)
                                                                              taatGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTgg                                              <  2:2707663/128‑1 (MQ=255)
                                                                              taatGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTgg                                              <  2:2568725/128‑1 (MQ=255)
                                                                                atGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGtt                                            <  2:3847130/128‑1 (MQ=255)
                                                                                atGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGtt                                            <  1:292711/128‑1 (MQ=255)
                                                                                 tGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTa                                           >  1:497881/1‑128 (MQ=255)
                                                                                 tGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTa                                           >  1:3532228/1‑128 (MQ=255)
                                                                                 cgATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGt                                                 >  2:1063402/2‑122 (MQ=255)
                                                                                   aTTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGa                                         <  1:3106281/128‑1 (MQ=255)
                                                                                    ttcttcTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAAACATTCATTCAGGACGTTGGTTAGaa                                        >  1:1558815/1‑128 (MQ=255)
                                                                                     tcttctATATAAATCATTAAATTAAGAAGTAGTAAATCTAACACCATCTAATTGAATAAATGTACGAATGAATGGTGTAATAACTGGATTTACTCTCTAAAAATTTCAAACAGGACGTTGGTTAGAAc                                       >  1:1482493/1‑128 (MQ=255)
                                                                                       ttctATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCATGACGTTGGTTAGAACGt                                     >  2:1833957/1‑128 (MQ=255)
                                                                                       ttctATATAAATCATTAATATCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGt                                     <  1:3606217/128‑1 (MQ=255)
                                                                                         ctATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAAACATTCATTCAGCACGTTGGTTATAACGttt                                   >  2:3420749/1‑128 (MQ=255)
                                                                                          tatataAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTTGAATAACTGTAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGtttt                                  <  1:676329/128‑1 (MQ=255)
                                                                                          tatataAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGtttt                                  >  2:1309282/1‑128 (MQ=255)
                                                                                           atataAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTa                                 <  1:2330334/128‑1 (MQ=255)
                                                                                           atataAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCAGTCAGTACGTTGGATAGAACGTTTTa                                 >  2:1722174/1‑128 (MQ=255)
                                                                                            tataAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTaa                                <  2:143315/128‑1 (MQ=255)
                                                                                             ataAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTaac                               >  1:3850416/1‑127 (MQ=255)
                                                                                             ataAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTaaa                               <  2:512455/128‑1 (MQ=255)
                                                                                              taAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTAGGTTAGAACGTTTTaaat                              >  1:1008269/1‑127 (MQ=255)
                                                                                                 aTCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCAt                           <  2:3117475/128‑1 (MQ=255)
                                                                                                 aTCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCAt                           >  2:3347070/1‑128 (MQ=255)
                                                                                                     ttAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCaaa                       >  1:79509/1‑128 (MQ=255)
                                                                                                     ttAATTTAAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGGCTGGTGTTATAACTGGATTTTCTCTCATAACCATTCAAGCAGGCCGTTGGTTAGAACGTTTTATACAATCaaa                       >  2:923711/1‑128 (MQ=255)
                                                                                                       aaTTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAg                     <  2:1575072/128‑1 (MQ=255)
                                                                                                        aTTTCAGAAGTTGTAAATCTAAGACCATCTAATTGTATTATTGGAAGAATGTCTGGTTGTATAACTGGAATTACTCTCATCACCATTCATTCAGGAGGTTGGTTAGAACGTTTTTAAGCATCAAAAGt                    <  2:2648172/128‑1 (MQ=255)
                                                                                                        aTTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGt                    <  1:3264020/128‑1 (MQ=255)
                                                                                                        aTTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGt                    <  2:1763264/128‑1 (MQ=255)
                                                                                                        aTTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAAATACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGt                    <  1:524729/128‑1 (MQ=255)
                                                                                                           tCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAACGCATCAAAAGTATc                 >  1:1625894/1‑128 (MQ=255)
                                                                                                                agtagtAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTCTAAAc            <  1:917865/128‑1 (MQ=255)
                                                                                                                  tagtaAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTCTAAACGt          >  1:3799990/1‑128 (MQ=255)
                                                                                                                   agtaCATCTAACACCATGTACATGAATTATTGGACGAATGTCTGGTGGAATAACTGTAATTACTCTCATAACGATTGATTCAGGTCGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTCTAAACGtt         <  1:1058753/128‑1 (MQ=255)
                                                                                                                   agtaAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTCTAAACGtt         >  1:3050915/1‑128 (MQ=255)
                                                                                                                    gtaAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCATAAGTTTCTAAACGttt        >  2:3009968/1‑128 (MQ=255)
                                                                                                                    gtaAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTCTAAACGttt        >  1:2987102/1‑128 (MQ=255)
                                                                                                                      aaaTCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTCTAAACGTTTta      >  1:3661286/1‑128 (MQ=255)
                                                                                                                       aaTCTAACAACATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTGAGAACGTTTAAAAGCATCAAAAGATTCTAAACGTTTgaa     >  2:3070961/1‑128 (MQ=255)
                                                                                                                         tCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCCGGACGTTGGTTAGAACGTTTTAAAGCATCAAATGTTTCTAACCGGTTtaata   >  2:2820727/1‑128 (MQ=255)
                                                                                                                             |                                                                                                                            
GTAACATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTACCACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTCTAAACGTTTTAATAA  >  CP016816/478613‑478862

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑