Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 420,163 2 bp→TT intergenic (‑446/+396) JCVSYN2_01895 ← / ← JCVSYN2_01900 30S ribosomal protein S10/tRNA‑Thr

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816420,1630AT99.4% 11764.5 / NA 3754intergenic (‑446/+397)JCVSYN2_01895/JCVSYN2_0190030S ribosomal protein S10/tRNA‑Thr
Reads supporting (aligned to +/- strand):  ref base A (13/10);  new base T (2012/1711);  total (2025/1729)
Fisher's exact test for biased strand distribution p-value = 8.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP016816420,1640AT98.7% 10546.0 / NA 3726intergenic (‑447/+396)JCVSYN2_01895/JCVSYN2_0190030S ribosomal protein S10/tRNA‑Thr
Reads supporting (aligned to +/- strand):  ref base A (11/39);  new base T (1998/1677);  total (2010/1716)
Fisher's exact test for biased strand distribution p-value = 4.90e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 3766 total aligned reads displayed.

TTCATTCATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTAAAATTAAAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑TTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTAAACGTATATTTA  >  CP016816/420039‑420287
                                                                                                                              ||                                                                                                                             
  cattcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATa                                                                                                                           <  1:9479069/128‑1 (MQ=255)
  cattcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATa                                                                                                                           <  2:5701575/128‑1 (MQ=255)
  cattcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATa                                                                                                                           <  2:4198153/128‑1 (MQ=255)
   attcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTtaataa                                                                                                                          >  2:5318310/1‑128 (MQ=255)
    ttcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAAc                                                                                                                         >  2:1293748/1‑128 (MQ=255)
     tcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACt                                                                                                                        <  2:8922216/128‑1 (MQ=255)
     tcatACTTTTAGTCTCATAAATATAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACt                                                                                                                        <  1:6999743/128‑1 (MQ=255)
       atACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGa                                                                                                                      <  2:7212742/128‑1 (MQ=255)
         aCTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAat                                                                                                                    <  1:3448116/128‑1 (MQ=255)
           ttttAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAatta                                                                                                                  >  2:7798056/1‑128 (MQ=255)
            tttAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATCTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAattat                                                                                                                 <  1:2805885/128‑1 (MQ=255)
             ttAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAattatt                                                                                                                <  2:231802/128‑1 (MQ=255)
             ttAGTCTCATAAAGCTAAGTTTCTAAAATTATTTTATCTTTTAAAATAATAATACTTTTATTAGTTGATATATTAGAAATCTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAattatt                                                                                                                <  2:2486398/128‑1 (MQ=255)
              tAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTAttt                                                                                                               <  1:3741494/128‑1 (MQ=255)
               aGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTAtttt                                                                                                              >  1:2946178/1‑128 (MQ=255)
                 tctcATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAACCTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTc                                                                                                            >  2:4944684/1‑128 (MQ=255)
                 tctcATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTc                                                                                                            >  1:5627484/1‑128 (MQ=255)
                  ctcATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCa                                                                                                           >  1:5798012/1‑128 (MQ=255)
                    cATAAATCTAAGTTTCTAAAATTATTTTATCATTTAATAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCaaa                                                                                                         <  2:5103911/128‑1 (MQ=255)
                     aTAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGaa                                                                                                                     >  2:3785001/1‑115 (MQ=255)
                     aTAAATCTAAGTTTATAAAATTATTATATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAAt                                                                                                        <  2:8142605/128‑1 (MQ=255)
                        aaTCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGt                                                                                                     <  1:9137803/128‑1 (MQ=255)
                        aaTCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAACAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGt                                                                                                     <  1:1797612/128‑1 (MQ=255)
                           cTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTaa                                                                                                  <  1:3113121/128‑1 (MQ=255)
                            tAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTaaa                                                                                                 <  2:3977615/128‑1 (MQ=255)
                            tAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTaaa                                                                                                 <  1:1615442/128‑1 (MQ=255)
                             aattttCTAAAATTATTTTATCATTTAAATAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAat                                                                                                <  2:6867691/125‑1 (MQ=255)
                               gTTTCTAAAATTATTTTATCATTTAAAAAAATAGTACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAatta                                                                                              >  2:9538436/1‑128 (MQ=255)
                               gTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAatta                                                                                              >  1:6094131/1‑128 (MQ=255)
                                tttCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAattat                                                                                             <  2:1535807/128‑1 (MQ=255)
                                 ttCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAattatt                                                                                            <  1:2678370/128‑1 (MQ=255)
                                  tCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAttt                                                                                           >  2:4429552/1‑128 (MQ=255)
                                  tCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAttt                                                                                           <  1:751127/128‑1 (MQ=255)
                                  tCTAAAATTATTATATCATTTAAAAAAATAATACTATTATTAGTTGATATATTAGATAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAAATGATTTATTTTTCAAATTTTTAAATTAttt                                                                                           <  1:9600794/128‑1 (MQ=255)
                                   cTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAtttt                                                                                          >  2:2886893/1‑128 (MQ=255)
                                   cTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAtttt                                                                                          <  1:2318996/128‑1 (MQ=255)
                                   cTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAtttt                                                                                          >  2:622917/1‑128 (MQ=255)
                                   cTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAtttt                                                                                          >  1:9004604/1‑128 (MQ=255)
                                   cTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAtttt                                                                                          <  2:3333430/128‑1 (MQ=255)
                                    tataaTTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAAGTGAATTATTTTTCAAATTGTTAAATTATTTTc                                                                                         <  2:8298200/125‑1 (MQ=255)
                                    tAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTc                                                                                         <  2:7963972/128‑1 (MQ=255)
                                    tAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTc                                                                                         <  1:6610532/128‑1 (MQ=255)
                                    tAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTAATAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTc                                                                                         <  2:6382126/128‑1 (MQ=255)
                                       aaTTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCaaa                                                                                      <  1:8698200/128‑1 (MQ=255)
                                            ttttATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGGACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTGAAATTGTTAAATTATTTTCAAATCAtt                                                                                 <  1:42212/128‑1 (MQ=255)
                                             tttATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTa                                                                                <  2:6637383/128‑1 (MQ=255)
                                              ttATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTAt                                                                               <  1:4656623/128‑1 (MQ=255)
                                              ttATCATTTAAAAAAATAATACTTATATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAAATTTTAATTTTAATAACTTAATTATTTTACAAATTATTAAATAATTTACAAATCATTaa                                                                               >  1:6292880/1‑127 (MQ=255)
                                              taaTCATAAAAAAAAATACAAATAATATTAGTTTATATATTAGAAAAATACTAGAACTTTTTTAAAAAATTTTTAATTTAAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTAt                                                                               <  1:5959833/126‑1 (MQ=255)
                                               tATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATc                                                                              <  2:9040454/128‑1 (MQ=255)
                                                aTCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCt                                                                             >  1:2140209/1‑128 (MQ=255)
                                                  cATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTAt                                                                           >  2:9247111/1‑128 (MQ=255)
                                                  cATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTAAAATCATTATCTAt                                                                           >  1:1980220/1‑128 (MQ=255)
                                                   aTTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTAtt                                                                          >  2:704509/1‑128 (MQ=255)
                                                    tttAAAAAAATAATACTTTAATTAGTTGATATATTAGAAAACTACTAGCACATTTTTAAAAAATTTTTAATTTTAATAACTGAATAATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTa                                                                         >  1:389283/1‑128 (MQ=255)
                                                       aaaaaaaTAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATc                                                                      >  1:8184731/1‑128 (MQ=255)
                                                         aaaaaTAATAATTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCaa                                                                    <  1:5319087/128‑1 (MQ=255)
                                                           aaaTAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAAtt                                                                  <  2:1965051/128‑1 (MQ=255)
                                                            aataatACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAAttt                                                                 <  1:6779834/128‑1 (MQ=255)
                                                             ataataCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAAtttt                                                                <  2:461820/128‑1 (MQ=255)
                                                             ataataCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAAtttt                                                                >  2:9386231/1‑128 (MQ=255)
                                                               aataCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATAATCTATTAATCAATTTTAt                                                              >  1:2475234/1‑128 (MQ=255)
                                                               aataCTTTTATTAGATGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTAt                                                              <  1:8538612/128‑1 (MQ=255)
                                                                ataCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATg                                                             >  1:1004074/1‑128 (MQ=255)
                                                                 taCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTAtct                                                            >  2:7494044/1‑126 (MQ=255)
                                                                 taCTTTTATTAGTTGATATATTAGAAAAATACTAGCAATTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGc                                                            <  1:784659/128‑1 (MQ=255)
                                                                  aCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAAttt                                                                 >  2:1699747/1‑122 (MQ=255)
                                                                  aCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCt                                                           >  1:6426765/1‑128 (MQ=255)
                                                                  aCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTAAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCt                                                           >  1:1331463/1‑128 (MQ=255)
                                                                    ttttATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTct                                                         >  2:478968/1‑127 (MQ=255)
                                                                     tttATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑t                                                      >  1:4976582/1‑128 (MQ=255)
                                                                     tttATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGATctt                                                        >  1:4335383/1‑126 (MQ=255)
                                                                       tattaGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑ttt                                                    >  2:5535301/1‑128 (MQ=255)
                                                                        tttaTTTGATATATTATAAAACTAAAATAAATTTATTAAAAAATTTTTAATTTTAATAAATTAATTTTTTTTAAAATTTTTAAATTATTTTAAAATCATTATCTATTAAAAAATTTTATGCTCG‑‑tttt                                                   <  2:8659671/127‑1 (MQ=255)
                                                                        attaGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑tttt                                                   >  2:2748231/1‑128 (MQ=255)
                                                                               gATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑ttttttttttt                                            >  1:5172099/1‑128 (MQ=255)
                                                                                  atatTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTAt                                                                               <  1:5446347/92‑1 (MQ=255)
                                                                                  atatTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑tttttttttttttt                                         >  2:3148391/1‑128 (MQ=255)
                                                                                  atatTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑tttttttttttttt                                         >  1:4482643/1‑128 (MQ=255)
                                                                                  atatTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTATATGCTCT‑‑tttttttttttttt                                         >  1:3598749/1‑128 (MQ=255)
                                                                                   tatTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTAACTATTAATCAATTTTATGCTCG‑‑ttttttttttttttt                                        <  1:928274/128‑1 (MQ=255)
                                                                                          aaaCTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTTTTAAATTATTTTCAAAACATTAACTATTAATCAATTTTATGCTCG‑‑gttttttttttttttacaaaaa                                 <  1:1151233/128‑8 (MQ=255)
                                                                                          aaaCTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑TTTTTTTTTTTTTTTTTACaaa                                 >  2:84672/1‑128 (MQ=255)
                                                                                            tattattttaattttttAAAAATATTTTTAATTTTATTAATTTATTAATTTTAAAAATTTTAAATTATTTTTAAATAATTATTTTTTAATTTTTTTTTTTTTCT‑‑TTTTTTTTTTTTTTTTTACaaaaa                               <  2:3586253/117‑1 (MQ=255)
                                                                                            aaaattagaaattttttAAAAAATTTTTTATTTTAATAACTTAATTATTTTTAAAATTTTTAAATTATTTTCAATTAATTATATATTAATCAATTTTATGCACG‑‑TTTTTTTTTTTTTTTTACAAAAat                              <  1:7354319/117‑1 (MQ=255)
                                                                                               aaaataaattttttAAAAAATTTTTAATTTTAATAACTTAATTATTTTTCAAATTTTTAAATTTTTTTAAAATCATTATCTATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTTTACAAAAata                             <  1:768305/120‑1 (MQ=255)
                                                                                                gtattaattttttAAATAATTTTTAATTTTATTAAATGAATTTTTTTTCAAATTTTTAAATTATTTTAAAATAAATAAATATTTATAAATTTTATTATCG‑‑ATTTTTTTTTTTTTTTACAAAAATATaa                          <  1:4846146/121‑1 (MQ=255)
                                                                                                ctaGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑TTTTTTTTTTTTTTTTTTAAAAAAaata                           >  2:2144563/1‑128 (MQ=255)
                                                                                                 tattaattttttAAAAAATTTTTAATTTTTAAAAATGAATTTTTTTTAAAATTTTTAAATTTTTTTTAAATAATTTTTTATTTAATAATTTTTTGTTAG‑‑TTTTTTTTTTTTTTTTTACAAAAATATaa                          <  2:8802782/122‑1 (MQ=255)
                                                                                                  aGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑TTTTTTTTTTTTTTTTTACAAAAAaataat                         >  1:8859838/1‑128 (MQ=255)
                                                                                                  aGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑TTTTTTTTTTTTTTTTTAAAAAAATATAat                         >  1:8366447/1‑128 (MQ=255)
                                                                                                  aGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTAG‑‑TTTTTTTTTTTTTTTTTAAAAAATATaaat                        >  1:3270619/1‑126 (MQ=255)
                                                                                                     aCTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑TTTTTTTTTTTTTTTTTAAAAAAATAAAattta                      >  1:4041107/1‑128 (MQ=255)
                                                                                                      cTTTTTTAAAAAATTTTTTATTTTAAATAATTAATTATTTTTAAAATTTTTAAATTATTTTAAAATAATTATATATTAATAAATTTTATTCTGG‑‑TTTTTTTTTTTTTTTTTACAAAAATATAatttat                     <  2:1101765/128‑1 (MQ=255)
                                                                                                      attttttAAAAAATTTTTAATTTTAAAAATTTAATTTTTTTTAAATTTTTTAATTTTTTTTAAAAAAATTAATTTTAAATAAATTTTATCTGGTTTTTTTTTTTTTTTTTTACAAAAATATAatttat                     <  1:8033116/127‑1 (MQ=255)
                                                                                                       ttttttAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTTTTAAAAAAATATAattta                      >  2:7355292/1‑128 (MQ=255)
                                                                                                       ttttttAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑TTTTTTTTTTTTTTTTTAAAAAAATATAatttatt                    >  1:1459658/1‑128 (MQ=255)
                                                                                                             aaaaaaTTTTAATTTTAAAAAATTAAATTTTTTTAAAAATTTTAAATTTTTTTTAAATAATAATCTAATTATAAATTTTATTGTTG‑‑TTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTaa             <  1:7166425/128‑1 (MQ=255)
                                                                                                              aaaaaTTTTTAATTTTTATAAATTAATTATTTTTCAAATTGTTAAATTATTTTCAAATAATTATCTATTAAATAATTTTATGCTCG‑‑TTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTaa             <  2:7639317/128‑1 (MQ=255)
                                                                                                              aaaaaTTTTTAATTTTAATTAATTAATTTTTTTTAAAATTTTTAAATTATTTTAAAATTATTATTTTTTTAAAAATTTTTTTTTTG‑‑TTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTaa             <  2:5959848/128‑1 (MQ=255)
                                                                                                                              ||                                                                                                                             
TTCATTCATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTAAAATTAAAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑TTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTAAACGTATATTTA  >  CP016816/420039‑420287

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: