BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP016816	420,157	2 bp→TT	intergenic (‑440/+402)	JCVSYN2_01895 ← / ← JCVSYN2_01900	30S ribosomal protein S10/tRNA‑Thr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP016816	420,157	0	A	T	99.5%	11804.4 / NA	3752	intergenic (‑440/+403)	JCVSYN2_01895/JCVSYN2_01900	30S ribosomal protein S10/tRNA‑Thr
Reads supporting (aligned to +/- strand): ref base A (9/10); new base T (2015/1715); total (2025/1727)
Fisher's exact test for biased strand distribution p-value = 6.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	CP016816	420,158	0	A	T	98.9%	11452.3 / NA	3754	intergenic (‑441/+402)	JCVSYN2_01895/JCVSYN2_01900	30S ribosomal protein S10/tRNA‑Thr
Reads supporting (aligned to +/- strand): ref base A (7/35); new base T (2018/1694); total (2025/1729)
Fisher's exact test for biased strand distribution p-value = 9.44e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 3905 total aligned reads displayed.

AAATTAAATTCATTCATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTAAAATTAAAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑TTTTATGCTCG‑‑TTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTAAACGTATATT  >  CP016816/420031‑420285

||

    taaattCATTCATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATttttaatt                                                                                                                                >  2:2175052/1‑128 (MQ=255)

      aattCATTCATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATttttaatttt                                                                                                                              >  2:2945556/1‑126 (MQ=255)

            ttcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAAc                                                                                                                        >  2:5113358/1‑128 (MQ=255)

            ttcatACTTTTAGTCTCATAAATCTAACTATCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAAc                                                                                                                        <  2:446125/128‑1 (MQ=255)

              catACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTg                                                                                                                      <  2:9533126/128‑1 (MQ=255)

               atACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGa                                                                                                                     <  2:7496637/128‑1 (MQ=255)

                 aCTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGaa                                                                                                                    >  1:4514834/1‑127 (MQ=255)

                   ttttAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAatta                                                                                                                 <  2:3789999/128‑1 (MQ=255)

                   ttttAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAatta                                                                                                                 >  2:7798056/1‑128 (MQ=255)

                    tttAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAattat                                                                                                                <  1:5615984/128‑1 (MQ=255)

                     ttAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAattatt                                                                                                               >  2:8917659/1‑128 (MQ=255)

                      tAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTATCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAAATAttt                                                                                                              >  1:6965418/1‑128 (MQ=255)

                         tctcATAAATCTAAGTTTCTAAAATTATTCTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTc                                                                                                           >  2:7647284/1‑128 (MQ=255)

                         gctcATAAATCTAAGTTTCTTAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTTATATATTATAAATCTACTAGCACTTTTTTAAAAAAATTTTAATTTTAATAACTGAATTATTATTc                                                                                                           <  1:7516924/127‑1 (MQ=255)

                           taaTAAATCTAAGTATCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCaa                                                                                                         <  1:7350826/126‑1 (MQ=255)

                             aTAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAAt                                                                                                       >  2:6667344/1‑128 (MQ=255)

                             aTAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTATATTAGTTGATATATTAGAAAACTACTATCACTTTTTAAAAAAATTATTAATTATAATAACTGAATAATTATTCaaaa                                                                                                       >  1:9498284/1‑127 (MQ=255)

                              tAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAAtt                                                                                                      >  1:9151592/1‑128 (MQ=255)

                              tAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATATTTCAAAtt                                                                                                      >  1:131633/1‑128 (MQ=255)

                              tAAATCTAAGTTTCTAAAATTATATTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTATAATTTTAATAACTGAATTATTTTTCAAAtt                                                                                                      <  2:2019924/128‑1 (MQ=255)

                               aaaTCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTAAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTg                                                                                                     <  2:7057754/128‑1 (MQ=255)

                                aaTCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGt                                                                                                    <  1:7689999/128‑1 (MQ=255)

                                aaTCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGt                                                                                                    <  2:5730508/128‑1 (MQ=255)

                                aaTCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGt                                                                                                    <  2:9237605/128‑1 (MQ=255)

                                 aTCTAATTTTATAAAATTATATTATCATTTAAAATAATAATACTTTTATTAGTTTATATATTAGAAAACTAATAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGtt                                                                                                   <  1:607985/128‑1 (MQ=255)

                                 aTCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATTCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTATAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGtt                                                                                                   <  1:642223/128‑1 (MQ=255)

                                  tCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTa                                                                                                  <  1:7141820/128‑1 (MQ=255)

                                     aaGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAat                                                                                               <  2:5551778/128‑1 (MQ=255)

                                      aGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAatt                                                                                              <  1:3740599/128‑1 (MQ=255)

                                      aGTTTCTAAAATTATTTTATCATTTAAAAAAATAATAATTTTATTAGTTGATATATTAGAAAACTACTAGAACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAatt                                                                                              <  2:4224437/128‑1 (MQ=255)

                                       gTTTCTATAATTATTTTATCATTTAAAAATATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAatta                                                                                             <  2:879404/128‑1 (MQ=255)

                                         ttCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAattatt                                                                                           >  2:8453992/1‑128 (MQ=255)

                                         ttCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAattatt                                                                                           >  1:8186797/1‑128 (MQ=255)

                                         ttCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAAATACTAGAACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATAATTTTTCAAATTGTTAAattatt                                                                                           >  2:1123562/1‑128 (MQ=255)

                                          tCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAttt                                                                                          >  2:2339512/1‑128 (MQ=255)

                                           cTAAAATTATTTTTTCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAATACTTCTAGCTCTTTTTTAATAAATTTTTTATTTTAATAACTGACTTATTTTTCAAATTGTTATATTAtttt                                                                                         <  1:5320877/128‑1 (MQ=255)

                                           cTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCTAATTCTTTAATTAtttt                                                                                         >  2:8647805/1‑128 (MQ=255)

                                           cTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAtttt                                                                                         >  1:8035574/1‑128 (MQ=255)

                                           cTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAAATTAATTATTTTTCAAATTTTTAAATAAtttt                                                                                         >  1:2471470/1‑128 (MQ=255)

                                            tAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTc                                                                                        <  2:7962913/128‑1 (MQ=255)

                                             aaaaTTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCa                                                                                       >  2:4953529/1‑128 (MQ=255)

                                               aaTTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCaaa                                                                                     >  2:7224565/1‑128 (MQ=255)

                                                attattTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAAATATTTTTCAAATTGTTAAATTATTTTCAAAt                                                                                    >  2:6239358/1‑128 (MQ=255)

                                                aatattTTATCATTTAAAAAAATTATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTATTAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAAt                                                                                    <  1:3597716/126‑1 (MQ=255)

                                                 ttattTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATc                                                                                   >  1:3445959/1‑128 (MQ=255)

                                                  tattTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCa                                                                                  >  1:8539329/1‑128 (MQ=255)

                                                    ctcttacgatctaaaaaaTAATACTTTTATTAGTTGAAATATTAGATAACTACTAGCACTTTTTTAAAAAATTTTTAATTATAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCAtt                                                                                <  2:8142304/116‑1 (MQ=255)

                                                        aTCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCt                                                                            <  1:6595639/128‑1 (MQ=255)

                                                         tCATTTAAAAAAATAATACTTTTATTTGTTGATATTTTAGAAAACTACTAGAACTTTTTTAATAAATTTTTAATTTTAATATCTGAATTATTTTTCATATTGTTAAATTATTTTCAAATCATTATCTa                                                                           <  1:6277946/128‑1 (MQ=255)

                                                         tCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTa                                                                           <  1:5784820/128‑1 (MQ=255)

                                                           aTTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTAtt                                                                         >  1:3098227/1‑128 (MQ=255)

                                                            tttAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTa                                                                        >  2:5991290/1‑128 (MQ=255)

                                                            tttAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTa                                                                        >  1:870299/1‑128 (MQ=255)

                                                                 aaaaaTAATACTTTTATTAGTAGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCaa                                                                   <  2:3600157/128‑1 (MQ=255)

                                                                    aataatACTTTTATTACTTGATAAATTAGAAAACTACTAGCACTTTTTTAAAAAATTATTAATTATAATAACTGAATTATTTTTCAAAATGTTAAAATATTTTCAAATCATTATCTATTAATCAA‑ttt                                                               <  2:1319072/128‑1 (MQ=255)

                                                                     ataataCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGaa                                                                                                                    <  2:4808366/75‑1 (MQ=255)

                                                                     ataataCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑tttt                                                              >  1:2801454/1‑128 (MQ=255)

                                                                     ataataCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑tttt                                                              >  2:9386231/1‑128 (MQ=255)

                                                                        ataCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑TTTTATg                                                           >  1:1004074/1‑128 (MQ=255)

                                                                         taCTTTTATTATTTGATATATTAGAAAACTAATAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTAAAATCATTATCTATTAATCAA‑TTTTAttc                                                          >  1:8878456/1‑126 (MQ=255)

                                                                          aattttATTAGTTTATATATTATAAAAATAATAGAAATTTTTTAAAAAATTTTTAATTTTAATAAATTAATTATTTTTCAAATTTTTAAATTATTTTCAAAACATTATCTATTAATCAA‑TTTTATGCt                                                         <  1:1459132/126‑1 (MQ=255)

                                                                            ttttATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑TTATATTCTCg                                                       >  1:5957344/1‑128 (MQ=255)

                                                                            ttttATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATATATTAATCAA‑TTTTATGCTCg                                                       >  1:4983462/1‑128 (MQ=255)

                                                                             tttATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑ttt                                                               <  1:9316495/119‑1 (MQ=255)

                                                                              ttattaGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑TTTTATGCTCt‑‑tt                                                   >  1:1791325/1‑128 (MQ=255)

                                                                              ttattaGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑TTTTATGCTCG‑‑tt                                                   >  1:19751/1‑128 (MQ=255)

                                                                               tattaGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑tt                                                                <  1:2942098/116‑1 (MQ=255)

                                                                               tattaGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑TTTTATGCTCt‑‑ttt                                                  >  1:4346041/1‑128 (MQ=255)

                                                                               tattaGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑TTTTATGCTCG‑‑ttt                                                  >  1:3265946/1‑128 (MQ=255)

                                                                                  tatattctatatTAGAAAACAAATAACACTTTTTTAAAAAATTTTTAAATTTAATAACTTAAATATTTTTCAAATTGTTAAAATATTTTCAAAACAATATCTATTAAACAA‑TTTTATGCTCG‑‑tttttt                                               <  2:3990349/121‑1 (MQ=255)

                                                                                  tacttGATAAATTATAAAACTACTATGAATTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTTTTAAATTATTTTCAAATCATAATATATAAATCAA‑TTTTATGCTCG‑‑tttttt                                               <  1:4852142/125‑1 (MQ=255)

                                                                                   aGTTTATATATTATAAAAATAATATTAATTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTAAAATTTTTAAATTATTTTCAAATCATTATCTATTAATCAA‑TTTTATGCTCG‑‑ttttttt                                              <  1:3903137/128‑1 (MQ=255)

                                                                                   aGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAatt                                                                                              >  1:4665514/1‑83 (MQ=255)

                                                                                     ttGTTATATTTGAAAAATAATTGAAATTTTTTAAAAAATTTTTAATTTTAATAATTTTATTATTTTTAAAATTGTTAAATTTTTTTAAAATAATTATATTTTAATAAA‑TTTTATGCTCG‑‑ttttttttt                                            <  2:6878262/128‑1 (MQ=255)

                                                                                       gATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑TTTTATGCTCG‑‑ttttttttttt                                          >  1:5172099/1‑128 (MQ=255)

                                                                                         tatatTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑TTTTATGCTCG‑‑ttttttttttttt                                        >  1:2674144/1‑128 (MQ=255)

                                                                                          atatTATAAAAATAATATTAATTTTTTAAAAAAATTTTAATTTTAAAAAATGAATTATTTTTAAAATTTTTAAATTATTTTAAAATCAAAATATATTAATAAA‑TTTTATTCTCG‑‑tttttttttttttt                                       <  1:9011980/128‑1 (MQ=255)

                                                                                          atatTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTc                                                                                        <  2:276932/82‑1 (MQ=255)

                                                                                          atatTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTAt                                                                              <  1:5446347/92‑1 (MQ=255)

                                                                                          atatTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑TTTTATGCTCG‑‑tttttttttttttt                                       >  2:3162497/1‑128 (MQ=255)

                                                                                            atTTGAAAACTACTAGAAATTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTTAAATTTTTAAATTATTTTAAAATCATTATCTATTAATCAA‑TTTTATGCGCG‑‑tttttttttttttttt                                     <  2:8292298/128‑1 (MQ=255)

                                                                                                gAAAACTACTAGCACTTTTTTAAAAAATTTTTAAATTTAATAACTGAATTATTTTTCAAATTGTTAAATTAATTTCAAAACATTATCTATTAATCAA‑TTTTATGCTCG‑‑tttttttttttttttacaaa                                 <  1:1158987/128‑6 (MQ=255)

                                                                                                  aaaCTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGATTTATTTGTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑TTTTATGCTCG‑‑‑TTTTTTTTTTTTTTTTACaaaa                              >  2:5345915/1‑128 (MQ=255)

                                                                                                  aaaCTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑TTTTATGCTCT‑‑TTTTTTTTTTTTTTTTTACaaa                               >  2:33465/1‑128 (MQ=255)

                                                                                                  aaaCTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑TTTTATGCTCG‑‑TTTTTTTTTTTTTTTTTAaaaa                               >  1:2133576/1‑128 (MQ=255)

                                                                                                   aaataatataaattttttAAAAAATTTTTAATTTTAATTAATTAATTTTTTTTAAAAATTTTTAATTTTTTTAAAATAATTATATATTAATAAA‑TTTTATTCTCG‑‑‑TTTTTTTTTTTTTTTTACaaaaa                             <  1:6763810/116‑1 (MQ=255)

                                                                                                    actactAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑TTTTATGCTCT‑‑TTTTTTTTTTTTTTTTTTaaaaaa                             >  2:2537397/1‑128 (MQ=255)

                                                                                                     atattatcaattttttAAAAAATTTTTAATTTTAATAAATTTATTATTTTTCAAATTTTTAAATTATTTTAAAATAATTATATATTAATCAA‑TTTTATGCCCGT‑TTTTTTTTTTTTTTTTTACaaaaa                             <  1:9623715/118‑1 (MQ=255)

                                                                                                        ctaGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAATTCATTATCTATTAATCAA‑TTTTATGCTCG‑‑TTTTTTTTTTTTTTTTTAAAAAAAtata                         >  2:4622215/1‑128 (MQ=255)

                                                                                                        ctaGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑TTTTATGCTCG‑‑TTTTTTTTTTTTTTTAaaaaaaaaataa                         >  1:2299330/1‑124 (MQ=255)

                                                                                                         taGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑TTTTATGCTCG‑‑TTTTTTTTTTTTTTTTTTTaaaaaaaaat                        >  2:9039535/1‑123 (MQ=255)

                                                                                                         taGCACTTTTTTAAAAAATTTTTAATTTAAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑TTTTATGCTCG‑‑TTTTTTTTTTTTTTTTTAAAAAAATATaa                        >  1:7854666/1‑128 (MQ=255)

                                                                                                          ttaatttttttAAAAAATTTTTTTATTTAATAAATTATTTATTTTTAAAATTTTTTAATTTTTTTAAAATAAATTTATATTAATAAATTTTTTTTTTTT‑‑TTTTTTTTTTTTTTTTTACAAAAATATaa                        <  2:1564247/123‑1 (MQ=255)

                                                                                                          aGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑TTTTATGCTCT‑‑TTTTTTTTTTTTTTTTTACAAAAAaatatt                       >  1:4225078/1‑126 (MQ=255)

                                                                                                          aGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑TTTTATGCTCG‑‑TTTTTTTTTTTTTTTTTAAAAAAATATAat                       >  1:8366447/1‑128 (MQ=255)

                                                                                                             aCTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑TTTTATGCTCG‑‑TTTTTTTTTTTTTTTTTAAAAAAATAAAattta                    >  1:4041107/1‑128 (MQ=255)

                                                                                                             aCTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑TTTTATGCTCG‑‑TTTTTTTTTTTTTTTTTAAAAAAAAATTattta                    >  2:6417413/1‑128 (MQ=255)

                                                                                                              cTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑TTTTATTCTCG‑‑TTATTTTTTTTTTTTTTAAAAAAATAAAatatat                   >  1:1609554/1‑128 (MQ=255)

                                                                                                               ttttttAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑TTTTATGCTCGTTTTTTTTTTTTTTTTTTTAAAAAAATATAattta                    >  2:7355292/1‑128 (MQ=255)

                                                                                                                         aaTTTTTAATTTTAATAAATTTATTATTTTTAAAATTTTTAAAATATTTTTAAATAATTATATATTAATAAA‑TTTTATTATCG‑‑TTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTAAACg        <  2:1734240/128‑1 (MQ=255)

||

AAATTAAATTCATTCATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTAAAATTAAAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑TTTTATGCTCG‑‑TTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTAAACGTATATT  >  CP016816/420031‑420285

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`