Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 478,738 C→A G301V (GGT→GTT)  JCVSYN2_02210 ← DNA‑directed RNA polymerase subunit beta'

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816478,7380CA99.2% 11248.8 / NA 3878G301V (GGT→GTT) JCVSYN2_02210DNA‑directed RNA polymerase subunit beta'
Reads supporting (aligned to +/- strand):  ref base C (16/16);  new base A (1980/1841);  total (2002/1876)
Fisher's exact test for biased strand distribution p-value = 8.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 3999 total aligned reads displayed.

GTAACATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTACCACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTCTAAACGTTTTAATAAT  >  CP016816/478613‑478863
                                                                                                                             |                                                                                                                             
 tAACATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTTATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTaacac                                                                                                                            >  2:9188276/1‑128 (MQ=255)
       aCGTTTTTCATTTTTTACTATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTTATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTa                                                                                                                      <  1:6522756/128‑1 (MQ=255)
       aCGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTa                                                                                                                      <  1:1709873/128‑1 (MQ=255)
        cGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTaa                                                                                                                     >  2:2462698/1‑128 (MQ=255)
           ttttCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTTATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTg                                                                                                                  >  1:1004887/1‑128 (MQ=255)
            attgattattTACAATACTTCCAGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGa                                                                                                                 <  2:1091795/124‑1 (MQ=255)
             ttCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTTATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGaa                                                                                                                >  2:8680551/1‑128 (MQ=255)
              tCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTTATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGaat                                                                                                               >  1:3695181/1‑128 (MQ=255)
               cATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGaata                                                                                                              <  2:8193196/128‑1 (MQ=255)
                 ttattTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGaataat                                                                                                            >  1:5136664/1‑128 (MQ=255)
                  tattTACTATAATTGATGTTGCACACATTTCTTTAACTTTTTTTAATCTTATATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAAtt                                                                                                           <  2:31892/128‑1 (MQ=255)
                   attTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTTATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGAAAATCTAACACCATCTAAATGAATAATTg                                                                                                          >  1:3884312/1‑128 (MQ=255)
                   attTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAGATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTg                                                                                                          <  2:7663858/128‑1 (MQ=255)
                    tttACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTTATTTCTAATAATGATTCATCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTgg                                                                                                         >  2:934477/1‑128 (MQ=255)
                    tttACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTgg                                                                                                         <  2:9490538/128‑1 (MQ=255)
                    tttACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTgg                                                                                                         >  1:4627822/1‑128 (MQ=255)
                      tACAATAATTGATGGTGCACCCATTTCTTTAACTTTTATTAATCTTTCATTTCTAATAATGATTAATCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGAc                                                                                                       >  2:3325748/1‑128 (MQ=255)
                        cAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGa                                                                                                     <  2:5159789/128‑1 (MQ=255)
                        cAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATATAATAAGTAGTAAATCTAACACCATCATATTGAATAATTGGaata                                                                                                     >  2:3621386/1‑125 (MQ=255)
                           taatTGATGTTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATg                                                                                                  <  2:1433098/128‑1 (MQ=255)
                           taatTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATg                                                                                                  >  2:1730744/1‑128 (MQ=255)
                              ttGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCt                                                                                               <  2:5956062/128‑1 (MQ=255)
                              atgctGGAGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGCTTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCt                                                                                               <  2:4148532/124‑1 (MQ=255)
                               tGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTTATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCtg                                                                                              >  1:5526459/1‑128 (MQ=255)
                               tGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTTATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCtg                                                                                              >  2:6639456/1‑128 (MQ=255)
                                   ggTGCACCCATTTCTTTAACTTTTTTTAATCTTTTATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCtggtgg                                                                                          >  1:4988014/1‑128 (MQ=255)
                                   ggTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCtggtgg                                                                                          >  1:6063317/1‑128 (MQ=255)
                                    gTGCACCGATTTCTTTAACTATTATTAATCTTTCATATCTATTAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGa                                                                                         <  1:8739740/128‑1 (MQ=255)
                                     tGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGaa                                                                                        <  1:8015456/128‑1 (MQ=255)
                                     tGCACCCATTTCTTTAACTTTATTTAATCTTTAATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTCACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGaa                                                                                        <  2:4987416/128‑1 (MQ=255)
                                      gCACCCATTTCTTTAACTTTTTTTAATCTTTTATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTaaca                                                                                                                             <  2:155691/90‑1 (MQ=255)
                                       cACCCATTTCTTTAACTTTTTTTAATCTTTTATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATGGAATAATTGGACGAATGTCTGGTGGAATa                                                                                      >  1:6206076/1‑128 (MQ=255)
                                       cACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACAATCTAATTGAATAATTGGACGAATGTCTGGTGGAATa                                                                                      >  2:5714551/1‑128 (MQ=255)
                                        aCCCATTTCTTTAACTTTTTTTAATCTTTTATTTCTAATAATGATTCTTCTATATTAATCATTAATTTCAGAAGTAGTAAATCTACCACCATCTAATTGAATAATTGGACGAATTTCTTGTGGAATaa                                                                                     >  2:6753640/1‑128 (MQ=255)
                                         cccATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAAc                                                                                    <  2:8341539/128‑1 (MQ=255)
                                          ccATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACt                                                                                   >  2:7868037/1‑128 (MQ=255)
                                          acATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACt                                                                                   <  1:8207739/127‑1 (MQ=255)
                                            aTTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCAGCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTgg                                                                                 >  2:6131214/1‑128 (MQ=255)
                                               tCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAAt                                                                              >  2:9030163/1‑128 (MQ=255)
                                                cTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAAtt                                                                             <  2:7291009/128‑1 (MQ=255)
                                                 tttAACTTTTTTTAATCTTTTATTTCTAATAATGATTCTTCTATATAAATCATTATTTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTa                                                                            <  2:3474304/128‑1 (MQ=255)
                                                  ttAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTAc                                                                           >  2:5518833/1‑128 (MQ=255)
                                                  ttAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTAc                                                                           <  2:2644636/128‑1 (MQ=255)
                                                      cTTTTTTTAATCTTTTATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACtctc                                                                       <  2:8053018/128‑1 (MQ=255)
                                                       tttttttAATCTTTTATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCa                                                                      <  2:715163/128‑1 (MQ=255)
                                                       tttttttAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCa                                                                      <  2:6272593/128‑1 (MQ=255)
                                                        ttttttAATCTTTTATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACtctc                                                                       >  1:5321124/1‑126 (MQ=255)
                                                           tttAATCTTTTATTTCTAATAAAGATTCTTCTATATAACTCATTAATTTCAGAAGTAGAAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGACTTACTCTCATatc                                                                  <  2:2309591/128‑3 (MQ=255)
                                                              aaTCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGATTAACGGGAATTAATCTCATAACcat                                                               >  2:1899525/1‑128 (MQ=255)
                                                               aTCTTTTATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACcatt                                                              <  1:8525825/128‑1 (MQ=255)
                                                               aTCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACcatt                                                              <  2:4468807/128‑1 (MQ=255)
                                                               aTCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACcatt                                                              >  1:2615473/1‑128 (MQ=255)
                                                                tCTTTTATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACcattc                                                             >  1:2081850/1‑128 (MQ=255)
                                                                tCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACcattc                                                             >  1:2832592/1‑128 (MQ=255)
                                                                        ttCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGa                                                     >  1:4055287/1‑128 (MQ=255)
                                                                         tCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTTGACGAATGTCTGGTTGAATAACTGGAATTACTCTCATAACCATTCATTCAGGAc                                                    >  1:8918373/1‑128 (MQ=255)
                                                                            aataatGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACcattca                                                            <  1:3325326/117‑1 (MQ=255)
                                                                             ataatGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTg                                                >  1:4435487/1‑128 (MQ=255)
                                                                             ataatGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTg                                                <  1:2987879/128‑1 (MQ=255)
                                                                               aatTATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGt                                              <  2:212436/128‑1 (MQ=255)
                                                                               aatGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGt                                              <  1:908452/128‑1 (MQ=255)
                                                                                atGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGtt                                             >  1:7107007/1‑128 (MQ=255)
                                                                                  gATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAg                                           >  2:2995502/1‑128 (MQ=255)
                                                                                  gATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACAATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAg                                           >  2:6974272/1‑128 (MQ=255)
                                                                                    ttcttcTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGaa                                         <  1:9631845/128‑1 (MQ=255)
                                                                                     tcttctATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATATCCATTCATTCAGGACGTTGGTTAGAAc                                        >  1:7471763/1‑128 (MQ=255)
                                                                                      cttctATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACGATTCATTCAGGACGTTGGTTAGAACg                                       >  2:7467876/1‑128 (MQ=255)
                                                                                       ttctATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATACACGACGTTGGTTAGAActt                                      >  1:1882569/1‑126 (MQ=255)
                                                                                         ctATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCCTCTAATTGACTAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGtgt                                    >  2:4651737/1‑126 (MQ=255)
                                                                                         ctATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGttt                                    >  1:2498906/1‑128 (MQ=255)
                                                                                           agaagaaTCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTa                                  >  1:696977/6‑128 (MQ=255)
                                                                                            tataAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATCATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTaa                                 <  2:8483167/128‑1 (MQ=255)
                                                                                            tataAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACcattcat                                                           >  1:1353061/1‑102 (MQ=255)
                                                                                             ataAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAAATGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTaaa                                <  2:128992/128‑1 (MQ=255)
                                                                                               aaaTCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGTATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGc                              >  2:5372559/1‑128 (MQ=255)
                                                                                                 agcATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTATGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCAt                            <  1:4262421/126‑1 (MQ=255)
                                                                                                   cATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCa                          >  1:1536152/1‑128 (MQ=255)
                                                                                                    aTTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCaa                         <  1:9445350/128‑1 (MQ=255)
                                                                                                    aTTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCaa                         <  1:6639456/128‑1 (MQ=255)
                                                                                                      tAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCaaaa                       <  1:5687315/128‑1 (MQ=255)
                                                                                                      tAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCaaaa                       <  1:4798694/128‑1 (MQ=255)
                                                                                                      tAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCaaaa                       <  2:2832592/128‑1 (MQ=255)
                                                                                                      tAATATCTGTAGTAGTAAATCTAACAGCTTCTAATTGAATAATTGGACGAATGTCTGGTGGAATATCTGGAATTACTCTCATAACCATTCATTGAGGACGTTGGTTAGAACGTTTTAAAGCATCaaaa                       <  1:783782/128‑1 (MQ=255)
                                                                                                         tttCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGtt                    >  2:3845233/1‑128 (MQ=255)
                                                                                                           tCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGCACGTTTTAAATCATCAAAAGTTTc                  >  2:430740/1‑128 (MQ=255)
                                                                                                            cAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCTTTCAGTCAGGCCGGTGGTGAGACCGTTTTAAAGCATCAAAAGTTTCt                 >  1:6326947/1‑128 (MQ=255)
                                                                                                            cAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTCt                 >  1:3155024/1‑128 (MQ=255)
                                                                                                             aGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGGATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCaaaa                       >  1:8366448/1‑121 (MQ=255)
                                                                                                             aGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGATTAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGGTTCTa                >  2:6462316/1‑128 (MQ=255)
                                                                                                              gAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTa                                                                            >  2:4000309/1‑67 (MQ=255)
                                                                                                                  tagtaAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTATAGCATCAAAAGTTTCGAAACGt           >  1:2324895/1‑128 (MQ=255)
                                                                                                                  tagtaAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCCTCAAAAGGTTCTAAACga           >  2:4811187/1‑127 (MQ=255)
                                                                                                                  tagtaAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTCTAAACGt           >  1:1143546/1‑128 (MQ=255)
                                                                                                                   agtaAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTCTAAACGtt          >  1:6798894/1‑128 (MQ=255)
                                                                                                                   agtaAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTCTAAACGtt          >  1:3506738/1‑128 (MQ=255)
                                                                                                                    gtaAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTCTAAACGttt         <  2:8898610/128‑1 (MQ=255)
                                                                                                                     taAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGATCGTTTTAAAGCATCAAAAGTTTCTAAACGtttt        >  1:9274624/1‑128 (MQ=255)
                                                                                                                       aaTCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTCTAAACGTTTtaa      <  1:5839175/128‑1 (MQ=255)
                                                                                                                        aTCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTCTAAACGTTTtaat     >  1:7637702/1‑128 (MQ=255)
                                                                                                                        aTCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTCTAAACGTTTtaa      >  1:7298976/1‑127 (MQ=255)
                                                                                                                             |                                                                                                                             
GTAACATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTACCACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTCTAAACGTTTTAATAAT  >  CP016816/478613‑478863

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑