Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 165,218 A→T intergenic (+182/‑360) JCVSYN2_00665 → / → JCVSYN2_00670 tRNA N6‑adenosine(37)‑N6‑threonylcarbamoyltransferase complex dimerization subunit TsaB/tRNA‑Ser

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816165,2180AT97.4% 10256.3 / 23.7 3535intergenic (+182/‑360)JCVSYN2_00665/JCVSYN2_00670tRNA N6‑adenosine(37)‑N6‑threonylcarbamoyltransferase complex dimerization subunit TsaB/tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base A (20/71);  new base T (2194/1250);  total (2214/1321)
Fisher's exact test for biased strand distribution p-value = 1.55e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 3688 total aligned reads displayed.

TTTACTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTAAAAGAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGT  >  CP016816/165093‑165346
                                                                                                                              |                                                                                                                                
tttACTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTtaa                                                                                                                                <  1:5744785/128‑1 (MQ=255)
tttACTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTtaa                                                                                                                                >  2:4972899/1‑128 (MQ=255)
tttACTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTtaa                                                                                                                                <  1:8261494/128‑1 (MQ=255)
  tACTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTtaaaa                                                                                                                              >  1:7053262/1‑127 (MQ=255)
    cTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTaaaaaa                                                                                                                            >  2:4293033/1‑128 (MQ=255)
     tttAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTTTATATGCAAATTTTTAAAAaat                                                                                                                           >  1:1303286/1‑128 (MQ=255)
     tttAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaat                                                                                                                           <  1:7430713/128‑1 (MQ=255)
     tttAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaat                                                                                                                           <  1:9534953/128‑1 (MQ=255)
       tAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTAAAAAaatta                                                                                                                         >  2:1854667/1‑128 (MQ=255)
          ttGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaattaatt                                                                                                                      >  1:178630/1‑128 (MQ=255)
           tGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAAttt                                                                                                                     <  2:198607/128‑1 (MQ=255)
           tGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAAttt                                                                                                                     <  1:4154873/128‑1 (MQ=255)
             tATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTAACTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGa                                                                                                                   <  2:7651782/128‑1 (MQ=255)
              aTTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGaa                                                                                                                  >  1:3304745/1‑128 (MQ=255)
              aTTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGaa                                                                                                                  <  1:8717024/128‑1 (MQ=255)
              aTTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGaa                                                                                                                  >  1:6332338/1‑128 (MQ=255)
              aTTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGaa                                                                                                                  >  1:6023406/1‑128 (MQ=255)
                 actttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAAttt                                                                                                                     <  2:3875908/122‑1 (MQ=255)
                 actttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACtt                                                                                                               <  2:7957243/128‑1 (MQ=255)
                   tttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGt                                                                                                             >  2:7827689/1‑128 (MQ=255)
                   tttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAAATAAAACTATTATCTTTTTAGATATTTTTTGTATATTCAAATTTTTAAAAAAATAATTTTAAAtttt                                                                                                             >  2:2334119/1‑126 (MQ=255)
                    ttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTa                                                                                                            >  1:9398666/1‑128 (MQ=255)
                    ttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTa                                                                                                            >  1:1657659/1‑128 (MQ=255)
                     tactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaa                                                                                                           >  2:9461505/1‑128 (MQ=255)
                     tactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaa                                                                                                           <  2:3018090/128‑1 (MQ=255)
                      actAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaaa                                                                                                          <  2:2886548/128‑1 (MQ=255)
                       ctAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaaaa                                                                                                         >  2:3673219/1‑128 (MQ=255)
                       ctAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaaaa                                                                                                         <  2:4622500/128‑1 (MQ=255)
                        tAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaaaaa                                                                                                        >  2:2521929/1‑128 (MQ=255)
                          gTTTATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAATACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaaaaaaa                                                                                                      <  2:2168183/128‑2 (MQ=255)
                           ttGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATTCAAATTTTTAAAAAATTAATTTGAACTTTTAAAAAATa                                                                                                     >  1:3930673/1‑128 (MQ=255)
                            tGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAg                                                                                                    >  2:9324344/1‑128 (MQ=255)
                                     ttAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttt                                                                                           >  1:7833509/1‑128 (MQ=255)
                                      tAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttt                                                                                          >  1:4706501/1‑128 (MQ=255)
                                      tAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttt                                                                                          >  1:8394541/1‑128 (MQ=255)
                                      tAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGAAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttt                                                                                          >  1:618837/1‑128 (MQ=255)
                                        gTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATATCttttttttttt                                                                                        >  1:5901070/1‑128 (MQ=255)
                                        gTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATATCttttttttttt                                                                                        >  2:8650857/1‑128 (MQ=255)
                                        gTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAAATATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATATCttttttttttt                                                                                        >  2:8466397/1‑128 (MQ=255)
                                         tttCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttt                                                                                       >  1:1922453/1‑128 (MQ=255)
                                         tttCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATATTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttt                                                                                       >  1:4559538/1‑128 (MQ=255)
                                         tttCATTAATTTATCGAAAATGTATTAATTATTAATAAACAAAAACTATTATCTTTTTATTTATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTTAACTTGTAAAAAATAGCtttttttttttt                                                                                       <  2:3323813/128‑1 (MQ=255)
                                           tCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATATCtttttttttttttt                                                                                     >  2:3516398/1‑128 (MQ=255)
                                           tCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttt                                                                                     >  1:8087817/1‑128 (MQ=255)
                                                 aTTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTAGTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGAgtgattgtttttcttttaaa                                                                               >  1:7252671/1‑125 (MQ=255)
                                                  tttATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTATATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATATCTTTTTTTTTTTTTTTTTTtaa                                                                              >  1:5443463/1‑127 (MQ=255)
                                                   ttATAGCATTTGTATTAATTAGTAATTAAATAAAAATTTTTTTTTTTTATTTTTTTTTTTTATATTAAAATTTTTTAAAAATTTATTTTAAATTTTAAAAAATATCTTTTTTTTTTTTTTTTTTtata                                                                             <  2:1392354/128‑1 (MQ=255)
                                                       cGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttttttttaaatttaa                                                                         >  2:1180822/1‑120 (MQ=255)
                                                       cGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTTTATTga                                                                         >  2:6134059/1‑127 (MQ=255)
                                                       cGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATATTTTGTATATGCAAATTTTTAAAAAATTAATTTAAACTTGTAAAAAATATCTATTTTATATTTTTTTTTATAAtcta                                                                        >  2:3182200/1‑125 (MQ=255)
                                                            ttGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTtatattttaatat                                                                    >  2:5464060/1‑119 (MQ=255)
                                                            ttGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTATTAAAAAATATATTTTTTTTTTTTTTTTTTATAATTAAAatat                                                                   >  1:6848333/1‑128 (MQ=255)
                                                             tGTTTGAATTATTAATTAAAAAAAAATATTATATTTTTTTATTTTTTTTTTATATTAAAATTTTTAAAAAATTAATTTTAACTTTTAAAAAATA‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAATATaa                                                                 <  2:5748165/128‑1 (MQ=255)
                                                               tttgtaatattAAGAAAAAAAAATTATTTTCTTTTTTTTTTTTTTTTTTTTATGCAAATTTTTAAAAAATTTATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATATaa                                                                 <  2:6332338/118‑1 (MQ=255)
                                                                aTGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATTTAAATATAAAttt                                                             >  2:6661328/1‑128 (MQ=255)
                                                                 tGAATTATTAATAAACTAAAAATTTAATTTTTTTATATATTTTTTTTATATTCAAATTTTTAAAAAATTAATTTGTAATTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAAtt                                                              <  1:3785220/128‑1 (MQ=255)
                                                                       aGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATTGAAATAATAATTTTaatta                                                       >  1:2076886/1‑124 (MQ=255)
                                                                         taaataacaaaaactaTTATTTTTTTTCATTTTTTTTTTATAATCAAATTTTTAAAAAATTAATTTTTAATTGTAAAAAAAATCCTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTAt                                                      <  2:2722445/123‑1 (MQ=255)
                                                                          aaGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTAAAATGTAAAAATAAATTTtatttatt                                                     >  1:3447791/1‑128 (MQ=255)
                                                                          aaGAAACATAAACTATTTTATTTTTTTATATTTTTTGTTTATTCTAATTTTTTAAAAATTAATTTGAAATTTTAAAAAAAA‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTAttt                                                    <  1:6658086/128‑1 (MQ=255)
                                                                             aaactaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTTAATGAAAATATAAATTTTACTATTttgt                                                 >  2:4825498/1‑128 (MQ=255)
                                                                             aaactaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTATTTAttttt                                                  >  1:3140988/1‑127 (MQ=255)
                                                                             aaactaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTAGTAAAAAATAGCTTTTTTTTTTTTTTTTTTAAAATATATAAAAAAATTTTAGTttttttt                                                  >  2:7090075/1‑127 (MQ=255)
                                                                              aactaaaaataTTATATTTTTTGATATTTTTTTTATATGCAAAATTTTAAAAAATTAATTTGAAATTTTAAAAAATA‑‑TGTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTttgtt                                                <  2:9163111/128‑1 (MQ=255)
                                                                               actaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTT‑‑TAATGTAAATATAAATTTTAGTTATttttttt                                               >  1:7965811/1‑127 (MQ=255)
                                                                                ctaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TCTTTTTTTTTTTTTTTATAATGAAAATATAAATTTTAGTTATtttttttt                                              >  1:2980372/1‑126 (MQ=255)
                                                                                   aaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATATTGTAAATTTAAATTTTAGttttttttttttat                                            >  2:7422256/1‑124 (MQ=255)
                                                                                   aaaattttttatttttATATATTTTTTTTTTTTGAAAATTTTTAAAAAAATAATTTTAAATTTTAAAAAATAC‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTaa                                          <  1:2221154/117‑1 (MQ=255)
                                                                                    aactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATTGAAATTAAAATTTTATTTATTTTTTTTTATaa                                          >  1:1151848/1‑128 (MQ=255)
                                                                                      cttttatGTTTTTTGATATTTTTTGTTTATTGAAATTTTTTAAAAATTAATTTTAACTTGTATAAAATAGCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAg                                         <  2:1661714/125‑1 (MQ=255)
                                                                                       tattatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAATTAGC‑‑‑TTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTAGGTATTTTTTTGTCTAATTAt                                      >  1:2382470/1‑128 (MQ=255)
                                                                                       tattatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGTAAAAATAAATTTTAGGTATTTTTTTTTATAAGTa                                       >  1:4885996/1‑128 (MQ=255)
                                                                                        attatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTAAAATGTAAAAATAAATTTTAGTTATTTTTTTGTATATGTATc                                     >  1:525755/1‑128 (MQ=255)
                                                                                           ttttttttATATATTTTTTTTATATTAAAATTTTTAAAAAATTAATTTTTAATTTTTAAAAATTTTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCt                                    <  2:6894388/125‑1 (MQ=255)
                                                                                           atCTTTTTATATATTTTTTTTATATGTAAATTTTTAAAAAATTAAATTTAACTTTTTAAAAAAATCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCt                                    <  1:854409/128‑1 (MQ=255)
                                                                                           atCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTAATTGTAAATAAAAATTTTATTTATtttttttaataattataa                                    >  2:5130601/1‑116 (MQ=255)
                                                                                           atCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTATTTATTTTTTTTTATAATtatata                                   >  1:145157/1‑128 (MQ=255)
                                                                                            tattttttTATTTTTTTTTTATATTTAAATTTTTTAAAAAATTATTTTTAAATTTTAAAAATATATTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCt                                    <  1:5613781/126‑1 (MQ=255)
                                                                                            tCTTTTTTTATATTTTTTGTATAATCAAATTTTTAAAAAATTTATTTTAAATTTTAAAAAATA‑‑‑TCTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTaaa                                 <  2:902185/128‑1 (MQ=255)
                                                                                               ttttAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATTTAAAAATAAATTTTATTTATTTTTTTTTATAATTTTATaaa                                 >  1:2625084/1‑128 (MQ=255)
                                                                                                tttttatatTTTTTTATATTCAAATATTTTAAAAATTAATTTTTACATGTAAAAAAAACC‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTaaaaaaa                             <  2:8096161/123‑1 (MQ=255)
                                                                                                tttAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTAAAATGAAAATATAATTTTTTGGTTTTTTTTTTTATAAGTAAATaaaaaa                              >  2:8250519/1‑128 (MQ=255)
                                                                                                  tAGATATTTTTTGTTTATTGAAATTTTTTAAAAATTAATTTGCAATTGTTAAAAATTTGTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTaaaaaa                              <  2:5957498/128‑1 (MQ=255)
                                                                                                      tatTTTTTGTTTATGCAAATTTTTAAAAAATTAATTTGTACTTGTAAAAAATA‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGaa                        <  1:7655437/128‑1 (MQ=255)
                                                                                                      aatTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAg                      <  2:8856466/127‑1 (MQ=255)
                                                                                                       tttttttttttATTCAAATTTTTTAAAAATTAATTTTAAATTTTAAAAAATA‑‑‑TCTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAg                      <  1:5067419/127‑1 (MQ=255)
                                                                                                       atTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTATTTATTTTTTTTTATAATTATATAAAAAAATAaaaa                       >  1:279029/1‑128 (MQ=255)
                                                                                                       atTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTAAAATTTAAATATAAATTTTATTTATTTTTTTTTATAATTATAAAAAAAAATATaaa                       >  1:9259937/1‑128 (MQ=255)
                                                                                                          ttttGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTAAAAATGGAAATAAAAATTTTAGTTATTTTGTTGTATTAGTATATAAAAAAATAAAAAgg                     >  2:7269120/1‑128 (MQ=255)
                                                                                                             tGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATTTAAATAAAAATTTTATTTATTTTGTATTAAAAGTATAAAAAAAAATATaaattaa                   >  2:4430625/1‑124 (MQ=255)
                                                                                                              ttataTTCAAATTTTTAAAAAATTAATTTGAAATTGTAAAAAATATCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGaaaa                 <  1:8902591/127‑1 (MQ=255)
                                                                                                                 tttttaaaTTTTTAAAAAATATATTTTAAATTTTTAAAAATTTTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAat              <  1:9095181/123‑1 (MQ=255)
                                                                                                                 ttttcAAATTTTTAAAAAATTAATTTTAAATTTTAAAAAATTTTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAat              <  2:5278028/124‑1 (MQ=255)
                                                                                                                  atGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATGTAAAAATAAATTTTAGTTATTTTTTTTTATAATTATTAAAAAAAAAATAAATAAAAAAata             >  2:1010960/1‑128 (MQ=255)
                                                                                                                  atGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATATTGGAAATATAAATTTTAGTTATTTTTTTTTATATGTATATAAAAAAATAAAAATTAAAAattatt           >  1:3633987/1‑128 (MQ=255)
                                                                                                                   tGCAAATTTTTAAAAAATTAATTTTAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATTATTTAAATATAAATTTTATTTATTTTTTTTTATAATTATAAAAAAAAATAATAAAAAAAAAATAtt           >  1:6512147/1‑128 (MQ=255)
                                                                                                                     cAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTAAAATGGAAATATAAATTTTAGTTATTTTTTTGTAAAATTAAAAAAAAAAATAAAAAGTAAAAAATAtttt         >  2:6459852/1‑126 (MQ=255)
                                                                                                                      aaaTTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTATGTATTTTGTTTTAAAAGTTTATAAAAAAATATAAATGAAAAAATTTTg          >  1:1012470/1‑128 (MQ=255)
                                                                                                                        aTTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATGTAAATAAAAATTTTTGGTTTTTTTTTGTATATTTATATAAAAAAATATAAATTAAAAAATATTTTTa       >  1:4337716/1‑128 (MQ=255)
                                                                                                                        aTTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑‑TTTTTTTTTTTTTTATAATGGAAATATAAATTTTATGTATTTTTTTTTATAAGTATCTAAAAAAATAGAAAGGAAAAAAATTTTTTaaaa  >  2:4071921/1‑127 (MQ=255)
                                                                                                                              |                                                                                                                                
TTTACTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTAAAAGAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGT  >  CP016816/165093‑165346

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: