Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 165,218 A→T intergenic (+182/‑360) JCVSYN2_00665 → / → JCVSYN2_00670 tRNA N6‑adenosine(37)‑N6‑threonylcarbamoyltransferase complex dimerization subunit TsaB/tRNA‑Ser

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816165,2180AT97.5% 5041.1 / ‑2.4 1686intergenic (+182/‑360)JCVSYN2_00665/JCVSYN2_00670tRNA N6‑adenosine(37)‑N6‑threonylcarbamoyltransferase complex dimerization subunit TsaB/tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base A (9/33);  new base T (1027/616);  total (1037/649)
Fisher's exact test for biased strand distribution p-value = 1.27e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1745 total aligned reads displayed.

TTTACTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTAAAAGAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTA  >  CP016816/165093‑165347
                                                                                                                              |                                                                                                                                  
tttACTTTAATTTTCTTCATTTATTATTTGATAATATTTATTTTATTTAATTTATATCACTTTTATTAATTATTAATAAACTAAAAATATTATCTTTTTAGATATTTTTTGTATATGAAAATTTTtaa                                                                                                                                  <  1:2192597/128‑1 (MQ=255)
tttACTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTtaa                                                                                                                                  <  1:1054269/128‑1 (MQ=255)
   aCTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTtaaaaa                                                                                                                               <  1:1594965/128‑3 (MQ=255)
       tAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaatta                                                                                                                           <  2:4589002/128‑1 (MQ=255)
           tGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAAttt                                                                                                                      <  1:4394098/128‑1 (MQ=255)
           tGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAAttt                                                                                                                      <  2:1743472/128‑1 (MQ=255)
           tGTATTACTTTAATAGTTTATAATATTTAGTTTAATTAATTTATCTCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAAttt                                                                                                                      <  1:3447491/128‑1 (MQ=255)
            gttttactttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTg                                                                                                                     <  1:2450177/125‑1 (MQ=255)
             tATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGa                                                                                                                    <  1:3190885/128‑1 (MQ=255)
             tATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGa                                                                                                                    <  2:1416953/128‑1 (MQ=255)
              aTTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGaa                                                                                                                   >  2:156151/1‑128 (MQ=255)
                   tttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGt                                                                                                              >  2:1068101/1‑128 (MQ=255)
                   tttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGt                                                                                                              >  2:1291213/1‑128 (MQ=255)
                   tttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGt                                                                                                              >  1:2110515/1‑128 (MQ=255)
                   tttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTCAACTTGt                                                                                                              >  2:811644/1‑128 (MQ=255)
                   tttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATATTTTTAGATATTTTTTGTATATGCAAATTTTTTAAAAATTAATTTGAACTAGt                                                                                                              >  2:2282255/1‑128 (MQ=255)
                    ttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTa                                                                                                             >  1:485769/1‑128 (MQ=255)
                        tAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaaaaa                                                                                                         >  2:2924565/1‑128 (MQ=255)
                        tAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaaaaa                                                                                                         <  1:3676184/128‑1 (MQ=255)
                         aGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaaaaaa                                                                                                        >  1:1666653/1‑128 (MQ=255)
                           ttGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATa                                                                                                      >  2:3087720/1‑128 (MQ=255)
                           ttGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATa                                                                                                      >  1:267549/1‑128 (MQ=255)
                           ttGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAAATATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTAGAACTTGTAAAAAATa                                                                                                      >  1:813245/1‑128 (MQ=255)
                           ttGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAAATATTATCATTTTAGATAATTTTTGTATATTCAAATTTTTAAAAAATTAATATGAACTTTTATAAAATa                                                                                                      >  1:3539381/1‑128 (MQ=255)
                                aataTTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttt                                                                                                 >  2:3537393/1‑128 (MQ=255)
                                aataTTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttt                                                                                                 >  1:3748630/1‑128 (MQ=255)
                                  tatTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTATTAAAAAATTAATTTGAACTAGTAAAAAATAGCttttt                                                                                               >  2:707015/1‑128 (MQ=255)
                                    tttAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttt                                                                                             >  2:2608883/1‑128 (MQ=255)
                                     ttAGTTTAAATAATTAATCGCACTTGTATTAATTATTAATAAAATAAAAATATTATTTTTTTATTTATTTTTTTTATATTCAAATTTTTAAAAAATTAATTTTAACTTGTAAAAAATAGCtttttttt                                                                                            <  2:2687796/128‑1 (MQ=255)
                                      tAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATAATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAATATAGCtttttttta                                                                                           >  1:4114739/1‑127 (MQ=255)
                                       tttttCATTAATTTTTAGCAATTTTATTAATTAGTAATAAAATAAAAATATTATCTTTTTATTTATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTTAACTTTTAAAAAATAGCtttttttttt                                                                                          <  1:4579488/126‑1 (MQ=255)
                                          ttCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttt                                                                                       >  1:926997/1‑128 (MQ=255)
                                          ttCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACCATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttt                                                                                       >  2:4073624/1‑128 (MQ=255)
                                           tCATTAAATTATCGCACTTGTATGAATTAGTAAGAAACTAAAAATATAATCTTTTTAGATATATATAGTAAATTCAAATTTTAAAAAAATTAATATGAACTTGTAAATAATAGCttttattattttat                                                                                      >  2:4145728/1‑126 (MQ=255)
                                            cATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttatttttttt                                                                                     >  2:3308175/1‑128 (MQ=255)
                                             aTTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttttt                                                                                    >  2:2520490/1‑128 (MQ=255)
                                             aTTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttttt                                                                                    >  1:4236887/1‑128 (MQ=255)
                                               tAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttttttta                                                                                  >  2:2356933/1‑127 (MQ=255)
                                               tAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttatttttttat                                                                                  >  2:1233094/1‑126 (MQ=255)
                                                aaTTTATTGCAATTTTATTTATTATTAATAAAAAAAAACTATTATCTTTTTATATATTTTTTTTTTATTCAAATTTTTAAAAAATTAAATTGAACTTTTAAAAAAAAGCattttttttttttttttat                                                                                 <  2:4019286/128‑3 (MQ=255)
                                                  tttATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTATTTTTTTTtataa                                                                               >  2:4357912/1‑127 (MQ=255)
                                                     aTCGCACTTGTATGAATTTGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttttttttaaatgt                                                                            >  1:1458189/1‑122 (MQ=255)
                                                      tagCACTTTTTTTAATTTGTAATAAACTAAAAATATTATATTTTTATATTTTTTTTTTTTATTCAAATTTTTAAAAAATTAATTTTAACTTGTAAAAAAAAGCTTTTTTTTTTTTTTTTTTATAATgg                                                                          <  2:2118261/126‑1 (MQ=255)
                                                      tCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTTAACTTGTAAAAAATAGCttttttttttttttttttaaatttt                                                                           >  1:3858847/1‑121 (MQ=255)
                                                           cTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTaatttaaatata                                                                      >  1:448637/1‑119 (MQ=255)
                                                            ttGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATATCTTTTTTTTTTATTTTTTatattttaaataaa                                                                     >  2:1939592/1‑118 (MQ=255)
                                                            ttGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGTAAata                                                                     >  2:918761/1‑128 (MQ=255)
                                                            ttGTAAGAATTAGTAAGAAAATAAAACATATATATTTTTATATATTTTTTTTATATTCAAATTTTTAAAAAATTAATTTTAAATTTTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGGAAAtata                                                                   <  1:1734464/128‑1 (MQ=255)
                                                                tttaatatttaataaAATAAAAATATTTTTTTTTATTTATTTTTTTTTAATTCAAATTTTTAAAAAAATAAATTTTAAATTTAAAAAAA‑‑TTCTTTTTTTTTTTTTTTTATAATGGAAATATAAAtttt                                                             <  2:1485867/119‑1 (MQ=255)
                                                                ttGAATTATTAAGAAAATAAAACTATTATATTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑GTTTTTTTTTTTTTTTTATAATGGAAATATAAAtt                                                               <  2:566380/127‑1 (MQ=255)
                                                                     ttatttataaaataaaactaTTATATTTTTATATTTTTTTTTTTTATTCAAATTTTTAAAAAATTAATTTGAAATTTTAAAAAATA‑‑‑TCTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGt                                                         <  1:717519/120‑1 (MQ=255)
                                                                      ttttaatcaaaaaaaaataTTATATTTTTAGAAATTTTTTGTTAATGCAAATTTTTAAAAAAATAAATTTCACTTTTTAAAAATA‑‑‑TGTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTa                                                        <  1:252931/116‑1 (MQ=255)
                                                                      ttttaataaAATAAAACTTTTATATTTTTATATATTTTTTGTATATTAAAATTTTTAAAAAATTAAATTTAAATTTTAAAAAAT‑‑‑TTCTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTa                                                        <  2:4509332/125‑1 (MQ=255)
                                                                       attaataaAATAAAAATATTATTTTTTTATTTATTTTTTTTTTTTTCAAAATTTTTAAAAATTAATTTTTAAATTTAAAAAATATCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGt                                                         <  1:3916440/126‑1 (MQ=255)
                                                                       aGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTAGTTa                                                        >  2:2783775/1‑128 (MQ=255)
                                                                        ttaataaAATAAAAATATTATATTTTTTTATATTTTTTGTTTATGCAAATTTTTAAAAAATTAATTTGAAATTGTAAAAAATAG‑‑GTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTAt                                                       <  2:3841562/127‑1 (MQ=255)
                                                                         tAAGAAAATTAAAATATTATTTTTTTATATATTTTTTTTATATTCAAATTTTTAAAAAATTAATTTTTACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTAt                                                       <  2:3575821/128‑1 (MQ=255)
                                                                         tAAGAAAAAAAAAATATTATCTTTTTTTTTATTTTTTTTATTTTTAAATTTTTAAAAAATTAATTTTAACTTTTTAAAAAAATCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTAt                                                       <  1:1999641/128‑1 (MQ=255)
                                                                             aaactaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTAT‑‑TTGAAATATAAATTTTATTTAtttttt                                                  >  1:3312312/1‑126 (MQ=255)
                                                                             aaactaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTATTTAtttttt                                                  >  1:4152834/1‑126 (MQ=255)
                                                                              aactaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGTAAAATAAAATTTTAGTTATTttgtt                                                 >  1:1377765/1‑128 (MQ=255)
                                                                              aacaaaaactaATATCTTTTTAGATTTTTTTTGTAAATTGAAATTTTTAAAAAATTAATTTTTAATTGTAAAAAAAACC‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTttgtt                                                 <  1:3610872/128‑1 (MQ=255)
                                                                                caaaaacttTTATCTTTTTTTTTTATTTTTGTTTTTTTAAATTTTTAAAAAAATTATTTTTACTTTTATAAAATAG‑‑GTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTtgttgt                                               <  2:3694411/126‑1 (MQ=255)
                                                                                 ttaatctaTTATCATTTTACTATTTTTTTTTATATTAAAATTTTTAAAAAATTAATTTGATATTGTAAAAAATA‑‑‑TCTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCt                                             <  2:3376818/123‑1 (MQ=255)
                                                                                 taaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑‑‑TTTTTTTTTTTTTATAATGGAAATTTAAATTTTAGGTATTTTTTTGTCTAAg                                          >  2:4436964/1‑128 (MQ=255)
                                                                                     actaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTATTTATTTTTTTTTATaat                                          >  2:3166117/1‑127 (MQ=255)
                                                                                      ctaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTAATAT‑‑AAATTTAAATTTTAGTTAATTTTTTTTATAATTa                                        >  2:1348117/1‑128 (MQ=255)
                                                                                         ttatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTAAATTTAATAATAAATTTTTTTTATTTTTTTTTATaatttt                                       >  1:2367745/1‑124 (MQ=255)
                                                                                         ttatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATGGAAAATTATATTTTAGGTTTTTTGTTGTATAAGttt                                       >  1:4325364/1‑126 (MQ=255)
                                                                                          tatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTTAATGTAAATTAAAATTTTATGTATtttttttaataattaaa                                      >  2:2445270/1‑117 (MQ=255)
                                                                                            tatttttATATATTTTTTGTATATTCAAATTTTTAAAAAATTAAATTTAAATTGTAAAAAATA‑‑‑TCTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTaaa                                  <  2:3912552/126‑1 (MQ=255)
                                                                                              tttttATATATTTTTTTTATATTCATATTTTTAAAAAATTAATTTGAAATTTTAAAAAATA‑‑‑TAATTTTTTTTTTTTTTTATAATTGAAATATAAATTTTATGTATTTTGTTGTCTAAGTATCTaaaaa                                <  2:2196493/128‑1 (MQ=255)
                                                                                                 ttAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTAGGTATTTTTTTTTATAAGTATCTaaaaaa                               >  2:3445485/1‑128 (MQ=255)
                                                                                                 ctattacttttttttATATTCAAATTTTTAAAAAATTAATTTGAAATTTTAAAAAAT‑‑‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAAt                             <  1:2287374/121‑1 (MQ=255)
                                                                                                   ttttatTTTTTTTATATTAAAATTTTTAAAAAAATAATTTTAACTTTTAAAAAATA‑‑‑TCTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAg                           <  1:1000910/125‑1 (MQ=255)
                                                                                                   aGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATA‑‑‑‑TCTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGa                          <  1:1291213/128‑1 (MQ=255)
                                                                                                    tttatTTTTTTTTTATTTAAATTTTTAAAAAATTAATTTTAAAATTTAAAAAATATCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATa                            <  1:2955333/126‑1 (MQ=255)
                                                                                                     tttttttttttATATTAAAATTTTTAAAAAATTAATTTGTACTTTTAAAAAATA‑‑‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGaa                         <  2:100534/125‑1 (MQ=255)
                                                                                                     atatTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTATAGTATTTTTTTTTATAATTATATAAAAAAATat                           >  2:1159144/1‑127 (MQ=255)
                                                                                                     atatTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATATAAATATAAATTTTATATATTTTTTTTTATAATTATAaaaaaaaaaata                          >  1:3147786/1‑124 (MQ=255)
                                                                                                         tttttGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTATAATTAAAATATAAATTTAATTTATTTTTTTGTATAATTTTAaaaaaaaaaaaaaaataa                    >  1:2853960/1‑124 (MQ=255)
                                                                                                          ttttttttttttaaaTTTTTAAAAAATTTATTTTTAATTGTAAAAAATA‑‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGa                     <  2:3237452/116‑1 (MQ=255)
                                                                                                          tttttttaatttaaaaTTTAAAAAAAATTATTTTAAATTTTAAAAAATTCC‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGa                     <  2:495101/116‑1 (MQ=255)
                                                                                                           tttttatattataaTTTTTAAAAAATTAATTTTAATTTGTAAAAAAAATCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGa                     <  1:1159144/116‑1 (MQ=255)
                                                                                                            ttttataTGTAAATTTTTAATAAATTAATTTGAAATTTTTAAAAATAGC‑‑TTTTTATTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGaaa                   <  2:4200304/125‑1 (MQ=255)
                                                                                                            ttGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTAAATTTAAAAAAAATTTTTAGTTATTTTTTTTTAAATTAATAAAAAAAAATAAAAAGaaaa                   >  2:3645014/1‑128 (MQ=255)
                                                                                                             tttttatttaaaTTTTTAAAAAATTAATTTTTAATTTTTAAAAATATTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGaaa                   <  2:3780582/119‑1 (MQ=255)
                                                                                                             tGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTT‑‑‑TAATGTAAATAAAAATTTTTGTTATTTTTTTGTAAAATTATCTAAAAAAATAAAAAGTaaaaa                 >  1:2598811/1‑128 (MQ=255)
                                                                                                              gTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATTTAAATTTTAATTTTAGGTATTTTTTTTTCTAAGTATCTAAAAAAATAGaatgaataaa                 >  1:379760/1‑120 (MQ=255)
                                                                                                              gTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTAT‑‑TGTAATTTAAAATTTTAGTTATTTTTTTTTATAATTATATAAAAAAATATAAAGTAAAAAat               >  2:3008901/1‑128 (MQ=255)
                                                                                                                atatGCAAATTTTTAAAAAATTTATTTGAAATTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAata              <  1:3039389/128‑1 (MQ=255)
                                                                                                                  atGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATTTAAATATAAATTTAATTTATTTTTTTTTATAATTATATAAAAAAATAaaaattaaaaatta              >  2:185030/1‑128 (MQ=255)
                                                                                                                   tGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATGGAAATTTAAATTTTAGGTTTTTTGTTGTATAAGTTTCTAAAAAAATATAAATGAAAAAatat             >  2:1659933/1‑128 (MQ=255)
                                                                                                                    ttaaaaTTTTAAATAATTAATTTTTAATTTTTAAAAAATTTTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAatat             <  1:1813974/126‑1 (MQ=255)
                                                                                                                      aaatttttTAAAAAATTTATTTGTAAATTTAAAAAAAA‑‑TGTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGt          <  1:574294/124‑1 (MQ=255)
                                                                                                                      aaaTTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTAGTTATTTTGTTTTCTAATTATCAAAAAAAATATAAAGTAAAAAATATTGTTa        >  2:618318/1‑128 (MQ=255)
                                                                                                                       aaTTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTAGTTATTTTTTTTTATAATTTTATAAAAAAATATAAATTAAAAAAtttttta         >  2:2025693/1‑125 (MQ=255)
                                                                                                                        aTTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTAT‑‑TGGAAATATAATTTTTAGTTATTTTTTTTTATATTTATTTAAAAAAATAAAAAGAAAAAAAAATTTTTaaatg    >  2:4282927/1‑126 (MQ=255)
                                                                                                                          ttttTAAAAAATTTATTTGTAAATGTTAAAAATA‑‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTaaa      <  1:4027534/128‑1 (MQ=255)
                                                                                                                          ttttAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAATTAAAAATTTTTTTTATTTTGTTGAATAAGTATCTAAAAAAATAAAAAGAAAAAAATTTTATTaaaat    >  1:1524013/1‑127 (MQ=255)
                                                                                                                              |                                                                                                                                  
TTTACTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTAAAAGAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTA  >  CP016816/165093‑165347

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: