BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP016816	32,707	A→T	I1067F (ATT→TTT)	JCVSYN2_00105 →	ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP016816	32,707	0	A	T	99.3%	6405.7 / NA	2036	I1067F (ATT→TTT)	JCVSYN2_00105	ABC transporter permease
Reads supporting (aligned to +/- strand): ref base A (4/11); new base T (1031/985); total (1037/999)
Fisher's exact test for biased strand distribution p-value = 7.10e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 2112 total aligned reads displayed.

TGTTACTCAACCTTTAACTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTATTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACCTAAATCAAATAACATTGAAA  >  CP016816/32580‑32833

    aCTCAACCTTTAACTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGAGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTaa                                                                                                                            <  2:3788760/128‑1 (MQ=255)

    aCTCAACCTTTAACTCAGTTTGTTGGAGAACTTTTAAAAACTATTATTAAAGATAAAGTGCAATCTACAGGAGAGTGAATAGAAAAGATCATAAACTTAATTTTAGGTTATTCTAGTTCAGTTTTTaa                                                                                                                            >  1:4627121/1‑128 (MQ=255)

      tCAACCTTTAACTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAAt                                                                                                                          <  1:1334454/128‑1 (MQ=255)

      tCAACCTTTAACTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAAt                                                                                                                          <  1:4152730/128‑1 (MQ=255)

        aaCCTTTAACTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCt                                                                                                                        >  2:4206597/1‑128 (MQ=255)

        aaCCTTTAACTCAGATTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCt                                                                                                                        <  2:935171/128‑1 (MQ=255)

             ttAACTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTAttt                                                                                                                   >  2:1595214/1‑128 (MQ=255)

             ttAACTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTAttt                                                                                                                   <  2:152738/128‑1 (MQ=255)

             ttAACTCAGTTTGTTGGAGAAATTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTAttt                                                                                                                   <  1:2223941/128‑1 (MQ=255)

              ttaCTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTa                                                                                                                  <  2:1824733/126‑1 (MQ=255)

                aCTCAGTTTGTTGGAGAACTTTTAATAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAAc                                                                                                                <  2:3421612/128‑1 (MQ=255)

                aCTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAAc                                                                                                                <  2:1057451/128‑1 (MQ=255)

                aCTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAAc                                                                                                                <  1:3847206/128‑1 (MQ=255)

                 cTCAGTTTGATGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACa                                                                                                               <  2:4503319/128‑1 (MQ=255)

                  tCAGTTTGTTTGAGAACTTTTAAAAACTATTATTAAAGATAAAGTGCAAACTAGAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACaa                                                                                                              <  1:1002126/128‑1 (MQ=255)

                    aGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGt                                                                                                            >  1:2525291/1‑128 (MQ=255)

                    aGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTGAAATCTTATTTAACAAGt                                                                                                            >  1:1213738/1‑128 (MQ=255)

                     gTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGATAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTACATTATTTAAATCTTATTTAACAAGTg                                                                                                           >  2:1901300/1‑128 (MQ=255)

                        tgttgGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAATGATCTTAAACTTAATTTTAGGTTATTATCATTCATGTTTTATATCTTATTTAACATGTGAAt                                                                                                        >  2:404572/1‑128 (MQ=255)

                         gtttGAGAAATTTTCAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGGTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAAtc                                                                                                       <  2:3188603/128‑1 (MQ=255)

                             gagaACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAACCTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGa                                                                                                   <  1:1487643/128‑1 (MQ=255)

                             gagaACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGa                                                                                                   <  2:3509033/128‑1 (MQ=255)

                             gagaACTTTTAAAAACTATATTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGa                                                                                                   <  1:2014934/128‑1 (MQ=255)

                              agaACTTTTAAAAACTATTTTTAAAGATGAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGaa                                                                                                  >  1:350287/1‑128 (MQ=255)

                               gaACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAag                                                                                                 >  1:3646791/1‑128 (MQ=255)

                                accTTTTAAAAACTAATATTAAAGATACAGTGCTAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATATTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAaga                                                                                                <  1:1109318/126‑1 (MQ=255)

                                aaCTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAATGAATAGATCTTAAACTGAATTTTAGGTTTTTCGCCTTCATGTTTTAAAGCTTATTTCACATGTGAATCTCGCaga                                                                                                >  1:2983276/1‑128 (MQ=255)

                                aaCTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAaga                                                                                                <  1:3145754/128‑1 (MQ=255)

                                aaCTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAaga                                                                                                >  1:706421/1‑128 (MQ=255)

                                aaCTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAaga                                                                                                <  1:4381595/128‑1 (MQ=255)

                                aaCTTTTAAAAACTAGTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTGAATTGTAGGTGATTCTCCTTCAGGGTTGAACTCGTATTTACCAAGTGAAGCTCGAaga                                                                                                >  1:4456930/1‑128 (MQ=255)

                                   ttttAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCt                                                                                             <  1:3732671/128‑1 (MQ=255)

                                   ttttAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCt                                                                                             <  2:1253106/128‑1 (MQ=255)

                                   ttttAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCt                                                                                             <  1:973680/128‑1 (MQ=255)

                                   gtttAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCt                                                                                             <  2:3337695/127‑1 (MQ=255)

                                    tttAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTc                                                                                            <  2:759238/128‑1 (MQ=255)

                                      tAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCaa                                                                                          >  1:3094034/1‑128 (MQ=255)

                                           aCTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGTAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTc                                                                                     >  2:3632086/1‑128 (MQ=255)

                                           aCTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGCAGAGCTCAATTTTc                                                                                     >  1:2725409/1‑128 (MQ=255)

                                            cTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCt                                                                                    >  2:3972622/1‑128 (MQ=255)

                                            cTATATTTAAAGATAAAGTGCAAACTACAGGTGAGTCAAAAGAAAAGATCTAAAACTTAATATTATGTTAGACTCCTTCTATTTTTAAAACATATTTAAAAAGTGAATCTCGATGAGCTGAATTTTCt                                                                                    >  1:3036555/1‑128 (MQ=255)

                                             tATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCtt                                                                                   >  2:2109956/1‑128 (MQ=255)

                                                 tttAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTgg                                                                               >  2:2270658/1‑128 (MQ=255)

                                                 ttaaaaGATATAGTGCAAAGTAGAGGTGAGTGTAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTgg                                                                               <  2:4283970/125‑1 (MQ=255)

                                                  ttAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGt                                                                              >  2:3571883/1‑128 (MQ=255)

                                                  ttAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGt                                                                              >  2:3040236/1‑128 (MQ=255)

                                                    aaaGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTg                                                                            >  2:4333407/1‑128 (MQ=255)

                                                    aaaGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTg                                                                            <  1:3987443/128‑1 (MQ=255)

                                                         tataGTTCAAACTACAGCTGAGTGAAAAGAAAAGAGCTTAAACTTAATTTTAGGTTATTCTCCATCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACaaa                                                                       <  1:3228251/125‑1 (MQ=255)

                                                          aaaGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCtttt                                                                                 >  2:3872926/1‑117 (MQ=255)

                                                          aaaGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACaaaa                                                                      >  2:2189590/1‑128 (MQ=255)

                                                           aaGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACaaaaa                                                                     <  1:609144/128‑1 (MQ=255)

                                                           aaGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATGTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTAGGTTGACaaaaa                                                                     >  1:798141/1‑128 (MQ=255)

                                                               gCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCtt                                                                                   <  2:1744680/110‑1 (MQ=255)

                                                               gCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAtaacaaa                                                                 >  2:1407418/1‑128 (MQ=255)

                                                               gCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAAGTTTCTTGTGGTTGACAAaaacaca                                                                 >  1:3919668/1‑126 (MQ=255)

                                                                   aCTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACta                                                              >  1:547938/1‑127 (MQ=255)

                                                                    cTACAGGTGAGTGAAATGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTATAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCCATTTTCTTTTGGTTTACTCAAACACACtaca                                                            >  1:4050328/1‑126 (MQ=255)

                                                                     tACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATaa                                                           >  1:900576/1‑128 (MQ=255)

                                                                      aCAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTTAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAAt                                                          >  2:850914/1‑128 (MQ=255)

                                                                         ggTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCATTTTTCTTTTGGTTGACACAAACAAACTATAATTca                                                       >  1:2085944/1‑127 (MQ=255)

                                                                            gagtgaAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTAAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGTTTGACAAAAACAAACTATAATTCcaca                                                    >  2:1975403/1‑128 (MQ=255)

                                                                            gagtgaAAAGAAAAGATCTTAAACTTAATTATAGGTTAATCTCCTTCATATATTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCcaca                                                    <  1:1863819/128‑1 (MQ=255)

                                                                             agtgaAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACaa                                                   <  1:2914095/128‑1 (MQ=255)

                                                                             agtgaAAAGAAAAGATCTTAAACTTAATTTTAGGTTAGTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACaa                                                   <  1:2411771/128‑1 (MQ=255)

                                                                              gtgaAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACaaa                                                  >  2:3097001/1‑128 (MQ=255)

                                                                               tgaAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATaa                                                           <  1:2316136/118‑1 (MQ=255)

                                                                               tgaAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACaaaa                                                 <  2:3252430/128‑1 (MQ=255)

                                                                               tgaAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTAATAAACAAGTGAATCTCGAAGAGCTCAATATTCTTTTGTTTGACAAAAACAAACTATAATTCCACaaaa                                                 >  2:490775/1‑128 (MQ=255)

                                                                                        aagaTCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCca                                                      >  1:1578773/1‑114 (MQ=255)

                                                                                         agaTCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTa                                       <  2:1321249/128‑1 (MQ=255)

                                                                                          gaTCTTAAACTTATTTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAg                                      <  1:3420867/128‑1 (MQ=255)

                                                                                            tCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAATAGCTCAATTTTCTTTTGGTTGACAAAAAAAAACTATAATTCCACAAAAAGATCAATTATCa                                    >  2:2448147/1‑128 (MQ=255)

                                                                                             cTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCaa                                   <  2:440318/128‑1 (MQ=255)

                                                                                               ttaaCTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACa                                                                         <  2:2051905/86‑1 (MQ=255)

                                                                                               tAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACTAAAAGCTCAATAAGCATCt                                 >  1:261625/1‑128 (MQ=255)

                                                                                               tAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAATATCAATTAGCAACt                                 >  2:299309/1‑128 (MQ=255)

                                                                                               tAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACt                                 <  1:2841482/128‑1 (MQ=255)

                                                                                               tAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACt                                 >  1:4250101/1‑128 (MQ=255)

                                                                                               tAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACt                                 <  2:4132067/128‑1 (MQ=255)

                                                                                               tAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATATTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACt                                 >  2:2921371/1‑128 (MQ=255)

                                                                                                 aaCTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTAt                               >  2:2767491/1‑128 (MQ=255)

                                                                                                    ttAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGATTCTCTAAGAGATCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTAtttt                            >  1:1425212/1‑128 (MQ=255)

                                                                                                    ttAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGTTTGACAAAAACAAAATATAATTCCACAAAAAGATCAAATAGCAACTAtttt                            >  2:1135668/1‑128 (MQ=255)

                                                                                                    ttAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTAtttt                            >  2:2659162/1‑128 (MQ=255)

                                                                                                     tAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTAttttt                           <  1:3322881/128‑1 (MQ=255)

                                                                                                         tttAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAAc                       <  2:678485/128‑1 (MQ=255)

                                                                                                           tAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAg                                      >  1:4554881/1‑111 (MQ=255)

                                                                                                           tAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACCt                     <  1:3583060/128‑1 (MQ=255)

                                                                                                           tAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGATCAATTTTCTTTTGGTTGACAAAAACAAACTAGAATTCAACAAAAAGATCAATTAGCAACTATTTTTAAACCt                     >  1:1651777/1‑128 (MQ=255)

                                                                                                            aGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACCTa                    >  2:593576/1‑128 (MQ=255)

                                                                                                              gTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACCTaaa                  <  2:2544111/128‑1 (MQ=255)

                                                                                                                 aTTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTTACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACCTAAATCa               >  2:2356710/1‑128 (MQ=255)

                                                                                                                 aTTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACCTAAATCa               >  2:3698493/1‑128 (MQ=255)

                                                                                                                   tctcCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACCTAAATCaaa             <  2:4590217/128‑1 (MQ=255)

                                                                                                                    ctcCTTCATTTTTTAAATGTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGGTGAGAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACCTAAATCAAAt            <  1:174514/128‑1 (MQ=255)

                                                                                                                        ttCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACCTAAATAAAATAACa        >  1:2133971/1‑128 (MQ=255)

                                                                                                                         tCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACCTAAATCAAATAACAt       <  1:1937490/128‑1 (MQ=255)

                                                                                                                         tCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACCTAAATCAAATAACAt       <  1:432231/128‑1 (MQ=255)

                                                                                                                         tCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACCTAAATCAAATAACAt       <  2:2865124/128‑1 (MQ=255)

TGTTACTCAACCTTTAACTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTATTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACCTAAATCAAATAACATTGAAA  >  CP016816/32580‑32833

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`