Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 478,738 C→A G301V (GGT→GTT)  JCVSYN2_02210 ← DNA‑directed RNA polymerase subunit beta'

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816478,7380CA99.0% 5360.6 / NA 1919G301V (GGT→GTT) JCVSYN2_02210DNA‑directed RNA polymerase subunit beta'
Reads supporting (aligned to +/- strand):  ref base C (12/8);  new base A (969/914);  total (991/928)
Fisher's exact test for biased strand distribution p-value = 5.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1970 total aligned reads displayed.

GTAACATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTACCACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTCTAAACGTTTTAATAA  >  CP016816/478613‑478862
                                                                                                                             |                                                                                                                            
gTAACATACGTTTTTCATTATTTACATTAATTGATGGTGCACCCATTTCTTGAACGTTTTTTAATCTTACATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTaaca                                                                                                                            >  1:944002/1‑128 (MQ=255)
gTAACATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTaaca                                                                                                                            >  1:2601078/1‑128 (MQ=255)
 tAACATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTaacac                                                                                                                           <  1:4326317/128‑1 (MQ=255)
    cATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCAt                                                                                                                        <  2:3486683/128‑1 (MQ=255)
    cATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCAt                                                                                                                        >  1:2415660/1‑128 (MQ=255)
    cATAAGTTTTTCATTATTTAAAATAATTGAAGGTGCACCAATTTATTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCAt                                                                                                                        <  2:1029078/128‑1 (MQ=255)
        cGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTaa                                                                                                                    <  2:2238428/128‑1 (MQ=255)
         gTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTACAGAAGTAGTAAATCTAACACCATCTAAt                                                                                                                   >  2:2301997/1‑128 (MQ=255)
            tttCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGa                                                                                                                <  2:3390910/128‑1 (MQ=255)
              tCATTATTTACTATAATTGATGGTTCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGaat                                                                                                              <  2:2345663/128‑1 (MQ=255)
               cATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGaata                                                                                                             >  2:3010002/1‑128 (MQ=255)
                attattTACAATAATTGATGGTGCACCAATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGaataa                                                                                                            <  1:3164927/128‑1 (MQ=255)
                 ttattTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGaataat                                                                                                           <  2:3700381/128‑1 (MQ=255)
                 ttattTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATATCAGAAGTAGTAAATCTAACACCATCTAATTGaataat                                                                                                           >  1:1227266/1‑128 (MQ=255)
                  tattTACAATAATTGATTGTGCTCACATTTCTTTATCTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTAAGAAGTAGTAAATCTAACACCATCTAATTGAATAAtt                                                                                                          <  2:1393303/128‑1 (MQ=255)
                   attTACTATAATTGATGTTGCACCCATTTCTTTATCTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTg                                                                                                         <  2:2526910/128‑1 (MQ=255)
                   attTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTg                                                                                                         <  1:2496513/128‑1 (MQ=255)
                    tttACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTgg                                                                                                        <  2:1854573/128‑1 (MQ=255)
                     ttACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGa                                                                                                       >  1:2972918/1‑128 (MQ=255)
                       aCAATAATTGATTGTGAAAACATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACg                                                                                                     <  2:2628273/128‑1 (MQ=255)
                       aCAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACg                                                                                                     >  1:1747377/1‑128 (MQ=255)
                        cAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGa                                                                                                    <  2:728467/128‑1 (MQ=255)
                        cAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGa                                                                                                    >  2:1553677/1‑128 (MQ=255)
                        cAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTATAATAATGATTCTTCTATATAAATCATTTATTTCAGAAGTAGTAAATCAAACAACATCTAATTGAATAATTTCACGa                                                                                                    >  1:2815979/1‑128 (MQ=255)
                            aatTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGt                                                                                                >  2:3838398/1‑128 (MQ=255)
                            aatTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATCCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGt                                                                                                <  1:2682876/128‑1 (MQ=255)
                              ttGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCt                                                                                              <  2:1789626/128‑1 (MQ=255)
                                gATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCtgg                                                                                            <  2:1099610/128‑1 (MQ=255)
                                gATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTCGACGAATGTCtgg                                                                                            >  2:3262301/1‑128 (MQ=255)
                                gATGGTGCACACATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCtgg                                                                                            <  2:2836752/128‑1 (MQ=255)
                                  tGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCtggtg                                                                                          <  1:1316692/128‑1 (MQ=255)
                                   ggTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCtggtgg                                                                                         <  1:4012449/128‑1 (MQ=255)
                                   ggTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCtggtgg                                                                                         >  2:539042/1‑128 (MQ=255)
                                     tGCACCCATTTCTTTATCTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACAATCTAATAGAATAATTGGACAAATGTatgatgtac                                                                                       >  1:1684905/1‑125 (MQ=255)
                                     tGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGaa                                                                                       <  1:4210916/128‑1 (MQ=255)
                                       cACCGATTTCTTTAACTTTTTTTAATCATTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATa                                                                                     <  2:1928290/128‑1 (MQ=255)
                                       cACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATa                                                                                     <  2:3774570/128‑1 (MQ=255)
                                       cACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATa                                                                                     >  2:4006872/1‑128 (MQ=255)
                                        aCCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGAGGAATGAATGTAGGAATaa                                                                                    >  1:3060111/1‑128 (MQ=255)
                                        aCCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATCGGACGAATGTCTGGTGGAATaa                                                                                    <  1:842339/128‑1 (MQ=255)
                                          ccATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGTTGGAATAACt                                                                                  >  2:3593663/1‑128 (MQ=255)
                                          ccATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACt                                                                                  >  1:3228705/1‑128 (MQ=255)
                                           cATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTg                                                                                 >  2:3923108/1‑128 (MQ=255)
                                           cATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTg                                                                                 >  2:2435755/1‑128 (MQ=255)
                                             tttCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGa                                                                               <  2:3182717/128‑1 (MQ=255)
                                                 tttAAATTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTa                                                                           <  2:1275519/128‑1 (MQ=255)
                                                  ttAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTAc                                                                          <  1:2296268/128‑1 (MQ=255)
                                                      cTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACtctc                                                                      >  2:2091378/1‑128 (MQ=255)
                                                        ttttttAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCAt                                                                    >  1:3799527/1‑128 (MQ=255)
                                                           tttAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAAc                                                                 <  1:3437920/128‑1 (MQ=255)
                                                             tAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACCCCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACca                                                               >  1:3940951/1‑128 (MQ=255)
                                                              aaTCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACcat                                                              <  2:1465487/128‑1 (MQ=255)
                                                               aTCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACcatt                                                             >  1:3491286/1‑128 (MQ=255)
                                                                 cTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACcattca                                                           >  2:2175068/1‑128 (MQ=255)
                                                                 cTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACcattca                                                           <  1:1001461/128‑1 (MQ=255)
                                                                  tttCATTTCTAATATTGATTCTTCTATATATATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGAAGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACcattcat                                                          <  2:4591841/128‑1 (MQ=255)
                                                                  tttCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACcattcat                                                          >  2:4638972/1‑128 (MQ=255)
                                                                    tCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCattcattc                                                        <  2:3106770/128‑1 (MQ=255)
                                                                     cATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCAttcattca                                                       >  2:2764086/1‑128 (MQ=255)
                                                                     cATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCAttcattca                                                       >  2:1710553/1‑128 (MQ=255)
                                                                         tCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGAc                                                   >  1:13791/1‑128 (MQ=255)
                                                                           taataaTGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGt                                                 <  1:646788/128‑1 (MQ=255)
                                                                           taataaTGATACTTCTATATAAATCATTAATTTCAGAAGTATTAAATGTAACACCATCTAATTGAATAAATGGAAGAAGGTCTGTTGGAGAAGCTGGAATTTCTCTAATAACAAgtcattcatctagt                                                 >  1:289689/1‑122 (MQ=255)
                                                                              tactGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATTATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTgg                                              <  2:939771/125‑1 (MQ=255)
                                                                               aatGATTCTTCTATATAAATCATTAATTTCAGAAGAAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGt                                             <  2:2897418/128‑1 (MQ=255)
                                                                                atGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTTGACGAATGTCTGGTGGAATAACTGGAATTACTCTAATAACCATTCATTACGGACGTTGGtt                                            >  1:3714750/1‑128 (MQ=255)
                                                                                 tGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGaa                                                                                       >  2:4165201/1‑84 (MQ=255)
                                                                                 tGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTa                                           >  2:4337263/1‑128 (MQ=255)
                                                                                    ttcttcTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCTTTCTGGCCGTTGTTTAGaa                                        >  1:553460/1‑128 (MQ=255)
                                                                                     tcttctATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAAc                                       >  1:2734271/1‑128 (MQ=255)
                                                                                       ttctATATAAATCATTAATTTCAGAAGTAGTAAATCTAACAACATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTAAATCAGGACGTTGCTTTGAACGt                                     >  1:1485213/1‑128 (MQ=255)
                                                                                        tctATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGtt                                    <  1:184749/128‑1 (MQ=255)
                                                                                         ctATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGttt                                   >  1:470155/1‑128 (MQ=255)
                                                                                         ctATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGGCTGGTGGAATAACTGGAATTACTCTCATAACCAGTCATTCAGGACGGTGGTTAGAACGgtt                                   >  1:1959165/1‑128 (MQ=255)
                                                                                           atataAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAAACATTCATTCAGGACGTTGGTTAGAACGTTTTa                                 >  2:4227597/1‑128 (MQ=255)
                                                                                           atataAATCATTAATTTCAGAAGTAGAAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGTAGGAATAACTGGAATTAGACTCATAGCCATTCATTCAGGACGTTGGTTATAACTATTTa                                 >  1:3584780/1‑128 (MQ=255)
                                                                                            aataAATCCTAAAGTCCTGAAGTCGTAAATCTAACACCCTCTACTTGAATACTTGGACGACTGTCTGGTGGAATAACTGGAATTACTCTCAAAACCATTCATTCAGGACGTTGGTGAGAACGTTTTaa                                <  1:2046239/127‑1 (MQ=255)
                                                                                             ataAATCATTAATTTCAGAAGTAGTAACTCTAACACGATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTaaa                               <  1:4121746/128‑1 (MQ=255)
                                                                                             ataAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTaaa                               <  1:1157257/128‑1 (MQ=255)
                                                                                             ataAATCATTAATTTCAGAAGTAGTAAATATAACACCATATAATTTAATAATTTGACGAATGTCTGGTGGAATAACTGGATTTACTCTAATAAACATTCATTAAGGCAGTTGGTTAGAACTTTTTaaa                               >  1:450320/1‑128 (MQ=255)
                                                                                              taAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAg                              <  2:4424893/128‑1 (MQ=255)
                                                                                               aaaTCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGc                             >  2:2716506/1‑128 (MQ=255)
                                                                                                 aTCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTaa                                >  2:1633048/1‑123 (MQ=255)
                                                                                                  tCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTTGTTAGAACGTTTTAAAGCATc                          >  2:4066722/1‑128 (MQ=255)
                                                                                                  tCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGGGTGGTGGATTAACTGGAATTGCTCTCCTATCCATTGAGGCAGTCCGTAGGTTAGAACGTTTTAAGGCagc                          >  1:2358331/1‑126 (MQ=255)
                                                                                                     ttAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGCCGTTGGGTAGAACGTTTTAACGCATCata                       >  1:4583456/1‑126 (MQ=255)
                                                                                                     ttAATTTCAGAAGTAGTAAATATAACCCCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATCACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGGTAGAACGTGTTAAAGCATCaaa                       >  1:1553244/1‑128 (MQ=255)
                                                                                                      tAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTTGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCaaaa                      <  1:1626573/128‑1 (MQ=255)
                                                                                                          ttCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGtt                                            >  2:633686/1‑102 (MQ=255)
                                                                                                           tCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTc                 <  2:4506325/128‑1 (MQ=255)
                                                                                                            cAGAAGTAGTATATCTAACCCCATCTAATTGAATAATTGGACGAATGTCTGGTCGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTGTCt                >  2:2948108/1‑128 (MQ=255)
                                                                                                              gAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTAGTCTCATAACCATTCATTCAGGACGTTGGTTATAACGTTTTACAGCATCAAAAGTTTCGaa              >  1:2140867/1‑128 (MQ=255)
                                                                                                                 gtagtaAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTCTAAACg           <  1:3348496/128‑1 (MQ=255)
                                                                                                                  tagtaAATCTAACACCATCTAATTGAATAATCGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTCTAAACGt          >  1:748370/1‑128 (MQ=255)
                                                                                                                   agtaAATCTAACACCATCTAATTGAATACTTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTCTAAACGtt         <  1:387205/128‑1 (MQ=255)
                                                                                                                   agtaAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACTTTGGTTAGAACGTTTTAAAGCATCAAAAGTATCTAAACGtt         >  2:830667/1‑128 (MQ=255)
                                                                                                                   agtaAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAATGCATCAAAAGTTTCTAAACGtt         >  2:1168547/1‑128 (MQ=255)
                                                                                                                     taAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCaaaa                      >  1:406553/1‑113 (MQ=255)
                                                                                                                     taAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTCTAAACGtttt       <  1:2248665/128‑1 (MQ=255)
                                                                                                                          cTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAATCATCAAAAGTTTCTAAACGTTTtaataa  >  1:4430464/1‑128 (MQ=255)
                                                                                                                             |                                                                                                                            
GTAACATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTACCACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTCTAAACGTTTTAATAA  >  CP016816/478613‑478862

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑