Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 20,023 2 bp→TA intergenic (‑744/‑392) JCVSYN2_00065 ← / → JCVSYN2_00070 hypothetical protein/tetracycline resistance ribosomal protection protein Tet(M)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP01681620,0230AT99.6% 2933.5 / NA 996intergenic (‑744/‑393)JCVSYN2_00065/JCVSYN2_00070hypothetical protein/tetracycline resistance ribosomal protection protein Tet(M)
Reads supporting (aligned to +/- strand):  ref base A (4/0);  new base T (577/415);  total (581/415)
Fisher's exact test for biased strand distribution p-value = 1.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP01681620,0240TA99.5% 2884.2 / NA 996intergenic (‑745/‑392)JCVSYN2_00065/JCVSYN2_00070hypothetical protein/tetracycline resistance ribosomal protection protein Tet(M)
Reads supporting (aligned to +/- strand):  ref base T (3/2);  new base A (578/413);  total (581/415)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1012 total aligned reads displayed.

GCAGGAAGAGCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATAATTTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGAAAAAACAAAAAAAG  >  CP016816/19901‑20145
                                                                                                                          ||                                                                                                                         
 cAGGAAGAGCAAGATAAAAGGTAGTATTTGTTTGCGATCACCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTtat                                                                                                                      <  1:217374/128‑1 (MQ=255)
   ggAAGAGCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAatttatatatttatat                                                                                                                    >  1:781677/1‑128 (MQ=255)
       gagCAAGATAAAGGGTAGTATTTGTTGGCGATCCCCCTAGAGACTTTAACATCTTCGGATAACAAAAACTATATTTTCTTTAATTTCTTTTTTTACATTCTATTTTTAATTTATAAATTtaatataaa                                                                                                                >  2:450154/1‑121 (MQ=255)
       gagCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTaaa                                                                                                                <  2:378892/128‑1 (MQ=255)
          cAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTaaaaaa                                                                                                             >  2:860705/1‑128 (MQ=255)
                aaaaGGTAGTATTTTTTGGCTATCACCCTAGAGTCTTTTACATCTTCTGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTaaa                                                                                                       <  2:206995/128‑1 (MQ=255)
                aaaaGGTAGTATTTGTTTTCGATCCACCTAGAGTCTTTTCCATCTTCGGAAAACAAAAAATAGTTTTTATATAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTaaaa                                                                                                       >  2:1388875/1‑128 (MQ=255)
                 aaaGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACATAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTATAAAATTTatat                                                                                                      >  1:1138608/1‑128 (MQ=255)
                 aaaGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAaat                                                                                                      <  2:48666/128‑1 (MQ=255)
                  aaGGTAGTATTTGTTGGCGATCCCCCTAGAGGCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAaatt                                                                                                     <  2:232554/128‑1 (MQ=255)
                  aaGCTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCTGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAaatt                                                                                                     <  1:600593/128‑1 (MQ=255)
                    ggTAGTATTTGTTTGCGATACCCCTAGAGTCTTTTCACTCTTCGGAAATCAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAaattat                                                                                                   <  2:1820486/128‑1 (MQ=255)
                       actaTTTGAGGGGGATCACCCTAGACTCTTTTACATCATCGGAACACAAAAACTATTTTTGCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAATATTTAaattataat                                                                                                <  2:1866649/126‑1 (MQ=255)
                        gtaTTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAaattataatt                                                                                               >  2:813737/1‑128 (MQ=255)
                        gtaTTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAaattataatt                                                                                               >  1:1813662/1‑128 (MQ=255)
                            ttgttgGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTtata                                                                                                                     >  2:1529753/1‑102 (MQ=255)
                             tgtgcgCGATCCCACTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTAtttt                                                                                          <  2:513127/123‑1 (MQ=255)
                              gttgGCGATCCCCCTAGAGTCTTTTACATCTTCGCTATACAAAATCTATTTTTTCGTTAATTACTTTTTTTACTTTCTATATGTAATTTATATATTTAAATTAAAAATGTTAAATTATAATTAtattt                                                                                         >  1:1364207/1‑128 (MQ=255)
                              gttgGCGATCCACCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTAttttt                                                                                         <  2:919185/128‑1 (MQ=255)
                               ttgGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACCAACACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATAATTATATTAAAAAAGTTAAATTATAATTATTtata                                                                                        >  1:1296548/1‑128 (MQ=255)
                                tgGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATTTTAAAAAATTTAATTTATAATTATATTtat                                                                                       >  2:751943/1‑128 (MQ=255)
                                tgGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTATTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTtat                                                                                       >  1:1584854/1‑128 (MQ=255)
                                  gCGATCCCCCTAGAGTCTTTTACATCTGCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAaattataa                                                                                                 >  1:2047202/1‑116 (MQ=255)
                                   cGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGc                                                                                    >  2:681836/1‑128 (MQ=255)
                                   cGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGc                                                                                    >  2:631426/1‑128 (MQ=255)
                                   cGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGc                                                                                    <  1:1418517/128‑1 (MQ=255)
                                   cGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGc                                                                                    >  2:1113865/1‑128 (MQ=255)
                                   cGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGc                                                                                    >  1:319277/1‑128 (MQ=255)
                                    gATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTaaa                                                                                                                <  1:378868/99‑1 (MQ=255)
                                    gATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCa                                                                                   <  2:1197553/128‑1 (MQ=255)
                                    gATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCa                                                                                   <  2:2003578/128‑1 (MQ=255)
                                    gATCCACATAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTATTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCa                                                                                   <  1:1545148/128‑1 (MQ=255)
                                       tccacTAGTGTCTTTTACATCTTCTGAATACAAAAAATATTTTTTATTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGt                                                                                <  1:1917407/124‑1 (MQ=255)
                                       cccgcTAGATGCTTTTTGATCTTCGGTAAACAAAAACTATTTTTTATTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGt                                                                                <  2:1595080/128‑1 (MQ=255)
                                        ccccTAGAGTCTTTTACATCATCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTg                                                                               <  2:1340128/128‑1 (MQ=255)
                                        ccccTAGAGGCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTg                                                                               <  2:2438313/128‑1 (MQ=255)
                                         cccTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTGTAATTTCTTTTTTTACTTTCTATTTTTAATTTATTTATTTATATTTAAAAATTTAATTTATAATTATTTTTATTGCTCGTGa                                                                              >  1:548208/1‑128 (MQ=255)
                                           cTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATa                                                                            >  2:1934393/1‑128 (MQ=255)
                                             agagTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAAt                                                                          >  2:210007/1‑128 (MQ=255)
                                             agagTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAAt                                                                          >  1:1739986/1‑128 (MQ=255)
                                              gagTCTTTTACATCTTCGTAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAAtt                                                                         <  1:1194368/128‑1 (MQ=255)
                                              gagTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTAATTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAAtt                                                                         <  1:712554/128‑1 (MQ=255)
                                               agTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTa                                                                        >  2:1903762/1‑128 (MQ=255)
                                               agTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTa                                                                        >  2:1452172/1‑128 (MQ=255)
                                               agTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTa                                                                        >  2:1475007/1‑128 (MQ=255)
                                                gTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTaa                                                                       >  1:1098090/1‑128 (MQ=255)
                                                     ttACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAACTTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGtt                                                                  <  1:2334499/128‑1 (MQ=255)
                                                       aCATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAg                                                                >  1:958030/1‑128 (MQ=255)
                                                       aCATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAg                                                                >  2:589535/1‑128 (MQ=255)
                                                         aTCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTg                                                                               >  2:1365427/1‑111 (MQ=255)
                                                         aTCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTg                                                              >  2:150440/1‑128 (MQ=255)
                                                           cTTAGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGaa                                                            <  2:1021826/128‑1 (MQ=255)
                                                            ttCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAAc                                                           >  2:316633/1‑128 (MQ=255)
                                                             tCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACa                                                          >  2:1773651/1‑128 (MQ=255)
                                                              cGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACaa                                                         <  1:639180/128‑1 (MQ=255)
                                                                gAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGa                                                       <  1:860705/128‑1 (MQ=255)
                                                                 aaaaCAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGaa                                                      >  1:283936/1‑128 (MQ=255)
                                                                  aaaCAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGaaa                                                     >  1:1628442/1‑128 (MQ=255)
                                                                   aaCAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGaaaa                                                    <  1:1692140/128‑1 (MQ=255)
                                                                     cAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACTTGATAATTAAAAGTTAGTGAACAAGAAAACa                                                  >  1:455555/1‑128 (MQ=255)
                                                                     cAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACa                                                  >  2:107274/1‑128 (MQ=255)
                                                                      aaaaaCTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAATTTAGTGAACAAGAAAACAg                                                 >  2:414951/1‑128 (MQ=255)
                                                                      aaaaaCTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAATACAg                                                 >  2:1641502/1‑128 (MQ=255)
                                                                      aaaaaCTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAg                                                 <  1:2221262/128‑1 (MQ=255)
                                                                       aaaaCTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACatt                                                >  1:904115/1‑126 (MQ=255)
                                                                       aaaaCTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGt                                                >  2:2364340/1‑128 (MQ=255)
                                                                         aaCTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGa                                              >  1:2069555/1‑128 (MQ=255)
                                                                          aCTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGaa                                             >  1:2007741/1‑128 (MQ=255)
                                                                          aCTATTTTTTCTTTAATTTCTTTTTTTACTTTATATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTTTTTTTATAGCACGTGATAATTAAAAATTATTGAACAATAAAACAGTGaa                                             >  2:556944/1‑128 (MQ=255)
                                                                          aCTATTATTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGaa                                             <  2:963205/128‑1 (MQ=255)
                                                                           cTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAg                                            <  1:2385621/128‑1 (MQ=255)
                                                                            tATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGc                                           >  1:1977891/1‑128 (MQ=255)
                                                                             aTTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTATTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCa                                          <  2:1731458/128‑1 (MQ=255)
                                                                              ttttttCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCAc                                         >  2:2031103/1‑128 (MQ=255)
                                                                              ttttttCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCAc                                         >  2:2285768/1‑128 (MQ=255)
                                                                              ttttttCTTTAATTTCATTTTTTACTTACTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCAc                                         <  1:2459510/128‑1 (MQ=255)
                                                                               tttttCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCAcc                                        >  1:1052971/1‑128 (MQ=255)
                                                                                ttttCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCa                                       <  1:1496908/128‑1 (MQ=255)
                                                                                ttttCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCa                                       >  2:116264/1‑128 (MQ=255)
                                                                                    cTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGttt                                   >  1:100276/1‑128 (MQ=255)
                                                                                    cTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGttt                                   >  1:124315/1‑128 (MQ=255)
                                                                                    cTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGttt                                   >  2:1262791/1‑128 (MQ=255)
                                                                                    cTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGttt                                   >  2:269404/1‑128 (MQ=255)
                                                                                    cTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGatt                                   >  1:2280302/1‑128 (MQ=255)
                                                                                    cTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATATTTATAGCACGTGATAATTAAAAGTTACTGAACAAGAACACAGTAAAGAACCAGtgt                                   >  2:1699158/1‑126 (MQ=255)
                                                                                      ttAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCt                                 <  1:2422698/128‑1 (MQ=255)
                                                                                           ttCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAAcc                            >  1:500666/1‑128 (MQ=255)
                                                                                             cTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCaa                          >  2:2155749/1‑128 (MQ=255)
                                                                                              tttttttACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTATATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCaaa                         <  1:1871994/128‑1 (MQ=255)
                                                                                              tttttttACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCaaa                         <  1:2113195/128‑1 (MQ=255)
                                                                                                   ttACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAaagaag                    >  2:35503/1‑128 (MQ=255)
                                                                                                    tACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGAACGTGATAATTATAAGTTAGTGAACAAGAAAACAGTGAATCACCAGTTTCTGAACCAAAagaaga                   >  2:1564566/1‑128 (MQ=255)
                                                                                                     aCTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAaagactac                  >  2:1238947/1‑124 (MQ=255)
                                                                                                     aCTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGAc                  >  2:2378130/1‑128 (MQ=255)
                                                                                                         tCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGaaa              <  2:1974864/128‑1 (MQ=255)
                                                                                                         tCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCCGAACCAAAAGAAGACGaaa              >  1:678152/1‑128 (MQ=255)
                                                                                                         tCTATTTTTAATTTATATATTTATATTAAAAAATATAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGaaa              <  2:897029/128‑1 (MQ=255)
                                                                                                          cTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTc                                  <  1:1659801/107‑1 (MQ=255)
                                                                                                            aTTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGaaaaaa           >  2:1293100/1‑128 (MQ=255)
                                                                                                                  aaTTTATATATTTATATTAAAAAATTAAAATTATAATTATTTTTATAGAACGTTATAATTAAAATTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGAAAAAACaaaaa     >  2:2228600/1‑128 (MQ=255)
                                                                                                                          ||                                                                                                                         
GCAGGAAGAGCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATAATTTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGAAAAAACAAAAAAAG  >  CP016816/19901‑20145

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: