BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP016816	281,616	C→T	Q100* (CAA→TAA)	JCVSYN2_01185 →	DNA‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP016816	281,616	0	C	T	99.7%	3843.4 / NA	1258	Q100* (CAA→TAA)	JCVSYN2_01185	DNA‑binding protein
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (601/653); minor base A (1/3); total (602/656)
Fisher's exact test for biased strand distribution p-value = 6.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1271 total aligned reads displayed.

ATGATTCTATTTCTAAAACTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAACAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATATTGTTGATCAAAATCAAATA  >  CP016816/281491‑281740

aTGATTCTATTTCTAAAACTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGTATTTACTAATTTCTCTAATTAATAAAATATAAACAAATCAAATAGATGAAAAATaa                                                                                                                            >  2:1223326/1‑128 (MQ=255)

      cTATTTCTAAAACTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTtata                                                                                                                      >  2:1840762/1‑128 (MQ=255)

      cTATTTCTAAAACTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTtata                                                                                                                      >  1:1723849/1‑128 (MQ=255)

       tATTTCTAAAACTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATaa                                                                                                                     <  1:2147196/128‑1 (MQ=255)

        aTTTCTAAAACTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATaaa                                                                                                                    >  1:987621/1‑128 (MQ=255)

           tCTAAAACTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAg                                                                                                                 >  1:2462494/1‑128 (MQ=255)

            cTAAAACTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATTAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGt                                                                                                                >  1:14329/1‑128 (MQ=255)

             tAAAACTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAttt                                                                                                               >  1:1056119/1‑128 (MQ=255)

              aaaaCTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGttt                                                                                                              <  2:877455/128‑1 (MQ=255)

              aaaaCTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGttt                                                                                                              <  2:1599897/128‑1 (MQ=255)

              aaaaCTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGttt                                                                                                              <  2:1168206/128‑1 (MQ=255)

               aaaCTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTa                                                                                                             <  2:1385604/128‑1 (MQ=255)

               aaaCTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTa                                                                                                             <  1:881574/128‑1 (MQ=255)

                aaCTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAAGTTAGAAAAAGATTaa                                                                                                            >  2:925609/1‑128 (MQ=255)

                 tcTAGTATATTATTATTTTATTTTGAGTCAAAATTAAATTTAAAACAAAAACAGTATTTACTAATTTCTCTAATTAATAAAATTGAATCAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTaaa                                                                                                           <  1:416276/127‑1 (MQ=255)

                 aCTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAATTTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTaaa                                                                                                           <  2:1013831/128‑1 (MQ=255)

                 aCTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTaaa                                                                                                           <  2:1757974/128‑1 (MQ=255)

                 aCTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTaaa                                                                                                           <  2:1690566/128‑1 (MQ=255)

                  cTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTaaaa                                                                                                          >  1:539847/1‑128 (MQ=255)

                     gTAAATTATTATTTAATTTAGAGACAAATTTACATTTAAATCAAAAACAGTATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAagag                                                                                                       <  1:2063279/128‑1 (MQ=255)

                     gTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAATCAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAagag                                                                                                       >  1:2385414/1‑128 (MQ=255)

                       aaaTTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGt                                                                                                     <  2:1485392/128‑1 (MQ=255)

                          ttattattTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGAt                                                                                                  >  1:1304351/1‑128 (MQ=255)

                           tattattTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGAtt                                                                                                 >  1:907098/1‑128 (MQ=255)

                               atttaatttaGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAATCAAATCAAATAGATGAAATATAATTTTTTTATAGTTTAAAAGAGttgatttgat                                                                                             >  2:2421389/1‑128 (MQ=255)

                                 ttaatttaGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTtgatga                                                                                           <  2:1809490/128‑1 (MQ=255)

                                   aatttaGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGaaa                                                                                         <  1:1764558/128‑1 (MQ=255)

                                    atttaGAGACAAAATTACATTTAAAACAAAAACAGGATTTCCTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAg                                                                                        <  1:935232/128‑1 (MQ=255)

                                    atttaGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAg                                                                                        >  2:390337/1‑128 (MQ=255)

                                         gagaCAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGATGATTTGATGAAAGTAtat                                                                                   >  1:1571077/1‑126 (MQ=255)

                                          agaCAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTtata                                                                                                                      <  2:2122434/92‑1 (MQ=255)

                                          agaCAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTaa                                                                                  <  2:2257945/128‑1 (MQ=255)

                                           gaCAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAAt                                                                                 >  2:796336/1‑128 (MQ=255)

                                             cAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGa                                                                               <  1:1164606/128‑1 (MQ=255)

                                              aaaaTTAGATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGAt                                                                              <  1:643253/128‑1 (MQ=255)

                                              aaaaTTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGAt                                                                              <  1:2019889/128‑1 (MQ=255)

                                              aaaaTTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGAt                                                                              >  2:1348858/1‑128 (MQ=255)

                                               aaaTTACATTTAAAACATAAATAGTATTTACTAATTTCTATAATTAATAAAATTGAAAGAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATc                                                                             <  1:500883/128‑1 (MQ=255)

                                               aaaTTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATc                                                                             >  1:1352910/1‑128 (MQ=255)

                                                  ttACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGga                                                                          >  2:2294699/1‑128 (MQ=255)

                                                   tACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCTgag                                                                         >  1:1507527/1‑128 (MQ=255)

                                                      aTTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATg                                                                      <  1:45725/128‑1 (MQ=255)

                                                       tttAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGt                                                                     <  1:792461/128‑1 (MQ=255)

                                                        ttAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGtt                                                                    <  2:1890705/128‑1 (MQ=255)

                                                          aaaacaaaaacaGGATTTACTAATTTCTCTAATTAATAAAATTGATACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTa                                                                  >  1:1379151/1‑128 (MQ=255)

                                                          aaaacaaaaacaGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTa                                                                  >  2:2081069/1‑128 (MQ=255)

                                                          aaaacaaaaacaGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTa                                                                  >  2:2165204/1‑128 (MQ=255)

                                                          aaaacaaaaacaGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTa                                                                  <  1:2293532/128‑1 (MQ=255)

                                                          aaaacaaaaacaGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGAGCGGAGATGTTTa                                                                  >  2:1652236/1‑128 (MQ=255)

                                                            aacaaaaacaGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCTGAGATGTTTATg                                                                >  1:234453/1‑128 (MQ=255)

                                                            aacaaaaacaGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGttt                                                                   <  1:1969583/125‑1 (MQ=255)

                                                            aacaaaaacaGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATg                                                                <  2:321384/128‑1 (MQ=255)

                                                            aacaaaaacaGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATg                                                                <  2:961236/128‑1 (MQ=255)

                                                               aaaaacaGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCaa                                                             <  2:2330168/128‑1 (MQ=255)

                                                                  aacaGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAAc                                                          <  2:29704/128‑1 (MQ=255)

                                                                   acaGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAAcc                                                         <  2:1137146/128‑1 (MQ=255)

                                                                     acgATTTACAAATTTCACAAATTACTAAAATTGAATCAAATCAAATAGATGAAAAATAATTAATAAATAGTTTAAAAGAGTTGATTTGATGAAAGTATTAATGATCGGTGATGTTTATGCAAAAACgg                                                       <  2:435640/126‑1 (MQ=255)

                                                                     aGGATTTACTAATTTCTCTAATTAATTAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCgg                                                       <  2:14329/128‑1 (MQ=255)

                                                                     aGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCgg                                                       <  1:1869034/128‑1 (MQ=255)

                                                                      gtaTTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATTAAAAATAATTTATAAATAGTTTTAAAGAGGTGATTTGATGAAATTATTAATGAGCTGATATGTTTATGCAAAACCggg                                                      <  1:840700/126‑1 (MQ=255)

                                                                             cTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGaa                                               >  1:1613663/1‑128 (MQ=255)

                                                                             cTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGaa                                               <  1:601983/128‑1 (MQ=255)

                                                                               aaTTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTAGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAAt                                             >  1:343594/1‑128 (MQ=255)

                                                                                   tctcTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTa                                         >  1:2104745/1‑128 (MQ=255)

                                                                                     tctAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCTGAGATGTTTATGCATAACCGGGAAGAGAATTGCTAGa                                       >  1:962625/1‑128 (MQ=255)

                                                                                         attaatAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGaaaaa                                   <  2:1716990/128‑1 (MQ=255)

                                                                                          ttaatAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAATAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATCCTAGAATAAc                                  >  1:2253378/1‑128 (MQ=255)

                                                                                          ttaatAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATTCTAGAAATAc                                  >  2:1990590/1‑128 (MQ=255)

                                                                                          ttaatAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAAc                                  <  1:1148372/128‑1 (MQ=255)

                                                                                           taataaAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCATAACCGGGAAGAGAAATGCTAGAAAATCa                                 >  2:698773/1‑128 (MQ=255)

                                                                                           taataaAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGATAAACa                                 >  1:1266472/1‑128 (MQ=255)

                                                                                           taataaAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACa                                 <  1:1000459/128‑1 (MQ=255)

                                                                                            aataaACTTGAAACAACTCAAATAGATGAATAATAATTTATAAAAAGTTTAAAAGAGGGGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACAt                                <  2:2380416/128‑1 (MQ=255)

                                                                                            aataaAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGTGAAGAGAAATGCTAGAAAAACAt                                >  1:392983/1‑128 (MQ=255)

                                                                                            aataaAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACAt                                >  2:838508/1‑128 (MQ=255)

                                                                                            aataaAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAAGGCTAGATAAACAt                                >  1:1923747/1‑128 (MQ=255)

                                                                                             ataaAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACAtt                               <  2:201775/128‑1 (MQ=255)

                                                                                             ataaAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACAtt                               <  1:1541474/128‑1 (MQ=255)

                                                                                              taaAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCTGGAAGAGAAATGCTAGAAAAACAttt                              >  2:2461444/1‑128 (MQ=255)

                                                                                                    tGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAAGATTTaaatat                        >  2:1570034/1‑125 (MQ=255)

                                                                                                    tGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTaaaaaa                        >  2:642977/1‑128 (MQ=255)

                                                                                                       aaCAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCTGGAAGAGAAATGCTAGAAAAACATTTATAAAatat                     >  1:1670651/1‑128 (MQ=255)

                                                                                                       aaCAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAACGATAGAAAAACATTTAAATActat                     >  2:127847/1‑128 (MQ=255)

                                                                                                        aCAAATCAAATAGAAGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAACCCGGGAAGAGAAATGATAGAAAAACATTTaaaaaaattt                    >  2:465824/1‑124 (MQ=255)

                                                                                                          aaatcaaatAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGGGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATAttgt                  <  1:121596/128‑1 (MQ=255)

                                                                                                            atcaaatAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATAttgttg                >  1:694748/1‑128 (MQ=255)

                                                                                                            atcaaatAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAACGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATAttgttg                >  2:515616/1‑128 (MQ=255)

                                                                                                             tcaaatAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTAGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGTAAGAGAAATGCTAGAAATACATTTAAAAAATATtgatga               >  1:1820802/1‑128 (MQ=255)

                                                                                                              caaatAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATAAATGATCGGAGATGTTTTTGGAAAACAGGGTCGAGAAATGCTAGACAAACAATTAAAAAATAatggttag              >  2:1531814/1‑123 (MQ=255)

                                                                                                               aaatATAAGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATATTGTTGatc             <  2:759238/128‑1 (MQ=255)

                                                                                                               aaatAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATATTGTTGatc             <  2:365536/128‑1 (MQ=255)

                                                                                                                aatAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATATTGTTGatca            >  2:1310855/1‑128 (MQ=255)

                                                                                                                    gATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATATTGTTGatcaaaat        >  1:270325/1‑128 (MQ=255)

                                                                                                                    gATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATATTGTTGatcaaaat        >  2:1929356/1‑128 (MQ=255)

                                                                                                                     aTGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATATTGTTGatcaaaatc       <  1:758145/128‑1 (MQ=255)

                                                                                                                      tGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAATACATTTAAAATATATTGTTGatcaaaatca      >  2:2213123/1‑128 (MQ=255)

                                                                                                                      tGAAAAATAATTTATAAAAAGTTTAAAAGAGGGGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATATTGTTGatcaaaatca      <  2:586929/128‑1 (MQ=255)

                                                                                                                       gAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATATTGTTGatcaaaatcaa     <  2:1099797/128‑1 (MQ=255)

                                                                                                                        aaaaaTAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATATTGTTGatcaaaatcaaa    <  1:1116028/128‑1 (MQ=255)

                                                                                                                          aaaTAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATATTGTTGATCAAAATCAAATa  <  1:2422356/128‑1 (MQ=255)

ATGATTCTATTTCTAAAACTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAACAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATATTGTTGATCAAAATCAAATA  >  CP016816/281491‑281740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`