Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 165,222 G→A intergenic (+186/‑356) JCVSYN2_00665 → / → JCVSYN2_00670 tRNA N6‑adenosine(37)‑N6‑threonylcarbamoyltransferase complex dimerization subunit TsaB/tRNA‑Ser

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816165,2220GA98.6% 2992.1 / 25.4 1019intergenic (+186/‑356)JCVSYN2_00665/JCVSYN2_00670tRNA N6‑adenosine(37)‑N6‑threonylcarbamoyltransferase complex dimerization subunit TsaB/tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base G (1/0);  major base A (665/334);  minor base . (3/11);  total (670/349)
Fisher's exact test for biased strand distribution p-value = 7.86e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1043 total aligned reads displayed.

CTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTAAAAGAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAAT  >  CP016816/165097‑165349
                                                                                                                              |                                                                                                                                  
cTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTaaaaaa                                                                                                                                  >  1:840608/1‑128 (MQ=255)
 tttAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaat                                                                                                                                 >  2:193208/1‑128 (MQ=255)
 tttAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaat                                                                                                                                 >  1:865201/1‑128 (MQ=255)
  ttAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATATTTTGTATATTCAAATTTTTAAAAaatt                                                                                                                               >  1:336687/1‑128 (MQ=255)
   tAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaatta                                                                                                                              >  1:726846/1‑128 (MQ=255)
   tAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaatta                                                                                                                              <  2:409497/128‑1 (MQ=255)
   tAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTGTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaatta                                                                                                                              >  2:1206100/1‑128 (MQ=255)
       tGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAAttt                                                                                                                          <  1:754193/128‑1 (MQ=255)
       tGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAAttt                                                                                                                          >  2:1904946/1‑128 (MQ=255)
       tGTATTACTTTACTAGTTGATAATATTTAGTTTCAATAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAAttt                                                                                                                          <  1:1003197/128‑1 (MQ=255)
        gTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTg                                                                                                                         <  1:403757/128‑1 (MQ=255)
         tATTACTTTACTAGTTTATAATATTTAGATTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGa                                                                                                                        <  2:1269397/128‑1 (MQ=255)
         tATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATAGCACTTGTATGAATTATTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTAAAAAAATTAATTTGa                                                                                                                        >  1:610322/1‑128 (MQ=255)
         tATTACTATACTAGTTGATAATATTTAGTTTCATTAATATATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGa                                                                                                                        <  1:68456/128‑1 (MQ=255)
               tttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTAGt                                                                                                                  >  2:2215702/1‑128 (MQ=255)
                 tactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAATAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaa                                                                                                                <  1:1653200/128‑1 (MQ=255)
                 tactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaa                                                                                                                <  1:1378304/128‑1 (MQ=255)
                 tactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGAAAATTTTTAAAAAATTAATTTGAACTTGTaa                                                                                                                >  1:1866340/1‑128 (MQ=255)
                 tactAGTTGATAATATATAGTTTCATTAATTTATCGAACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaa                                                                                                                <  1:1790000/128‑1 (MQ=255)
                  actAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaaa                                                                                                               >  1:2424073/1‑128 (MQ=255)
                   ctAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaaaa                                                                                                              <  2:14109/128‑1 (MQ=255)
                   ctAGTTGATAATATTTAGTATCATTAATTTATCGAACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTAAAAAATTAATTTGAACTTGTaaaaa                                                                                                             <  1:117617/128‑1 (MQ=255)
                    tAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTTAACTTGTaaaaa                                                                                                             >  1:52660/1‑128 (MQ=255)
                      gTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAAt                                                                                                           >  2:765835/1‑128 (MQ=255)
                       ttGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTTAAAAATTAATTTGAACTTGTAAAAAATa                                                                                                          >  2:554609/1‑128 (MQ=255)
                         gATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGc                                                                                                        >  2:1885561/1‑128 (MQ=255)
                             atatTTAGTTACATTTATTTATCGCAATTTTTTGAATTAGTAATTAAATAAAAAATTTATATTTTTAGTTAATTTTTTTATATGAAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑attt                                                                                                  <  1:2269845/128‑1 (MQ=255)
                               atTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑tttttt                                                                                                >  2:1661212/1‑128 (MQ=255)
                               atTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑tttttt                                                                                                >  1:2072760/1‑128 (MQ=255)
                               atTTAGTTTAATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTAATATCTTTTTAGATATTATTTGTATACTCAAATTTTTAATAAATTAATTTGAACTTGTAAAAAATAGC‑‑ttttta                                                                                                >  2:1623078/1‑127 (MQ=255)
                                     tttCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑tttttttttttt                                                                                          >  2:94413/1‑128 (MQ=255)
                                     tttCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTCAACTTGTAAAAAATAGC‑‑ttttattttttt                                                                                          >  2:104199/1‑128 (MQ=255)
                                      ttCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑ttttatttttttt                                                                                         >  1:1432489/1‑128 (MQ=255)
                                       tCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑tttttttttttttt                                                                                        >  1:1582257/1‑128 (MQ=255)
                                        cATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑ttttttttttttttt                                                                                       >  2:882967/1‑128 (MQ=255)
                                        cATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑ttatttttttttttt                                                                                       >  2:500100/1‑128 (MQ=255)
                                          ttAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑ttttttttttttttttt                                                                                     >  2:1849028/1‑128 (MQ=255)
                                              tttATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑ttttttttatttttttttaaa                                                                                 >  2:515107/1‑125 (MQ=255)
                                                  ttgCAATTGTATGTATTAGTTATAAAATAAAACTATTTTTTTTTTATATATTTTTTTTATATTCAAATTTTTAAAAAATTAATTTTAAATTTTAAAAAATATC‑‑TTTTTTTTTTTTTTTTTTATAATgg                                                                            <  2:2153509/126‑1 (MQ=255)
                                                   ctaaattttattaattattaatTAAATAAAAATATTATCTTTTTATATATTTTTTTTATATGCAAATTTTTAAAAAATTAATTTTAAATTTTAAAAAATATC‑‑TTTTTTTTTTTTTTTTTTATAATGGa                                                                           <  1:432965/120‑1 (MQ=255)
                                                      aCTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATAAGCAAATTTTTAAAAAATTAATTTGAACTTCTAAAAAATAGC‑‑TTTTTTTTTTATTTTTTTaatattaaaat                                                                         >  2:2257971/1‑121 (MQ=255)
                                                        ttGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATATC‑‑TTTTTTTTTTTTTTTTTTTTAATTGAAatta                                                                      >  1:1060446/1‑126 (MQ=255)
                                                        ttGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTATAAttaaaaata                                                                      >  1:79534/1‑120 (MQ=255)
                                                         tGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTTTTaaattatatat                                                                      >  2:465382/1‑119 (MQ=255)
                                                            ttGAATTATTAATAAAATAAAACTAATATCTTTTTAGATATTTTTTGTATATGGAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAG‑‑GTTTTTTTTTTTTTTTTTATAATGGAAATATAAAtt                                                                 <  2:684997/127‑1 (MQ=255)
                                                             ttaaTTATTAACTAAATAAAAATATTATATTTTTATTTATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATATC‑‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAAtt                                                                 <  2:1156892/126‑1 (MQ=255)
                                                              gAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTATAATTTAAAAATAAAttt                                                                >  1:1473320/1‑128 (MQ=255)
                                                               tatttttattaaacaaaaattaTTATTTTTTTTTATATTTTTTTTATATTCAAATTTTTAAAAAATTAATTTTAAATTTTAAAAAATA‑‑‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTa                                                              <  2:1521519/118‑1 (MQ=255)
                                                               atttAGTCATAAACTTAAACTATTTATTTTTTAGATTTTTTTTGTTTATGCAATTTTTTAAAAATTTTAATTGAACTTTTAAAAAATAGC‑‑‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAg                                                             <  1:1299598/126‑1 (MQ=255)
                                                                attattAATAAAATTAAAATATTATATTTTTATTTTTTTTTTTTTTATTTAAATTTTTAAAAAATTAATTTTTAATTTTTAAAAATTTC‑‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTa                                                              <  2:1716342/128‑1 (MQ=255)
                                                                aTTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑‑TTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTAgt                                                            >  1:1162958/1‑127 (MQ=255)
                                                                 ttattaattaaataaaaataTTATATTTTTTGTTATTTTTTTTTTATTAAAATTTTTAAAAAATTAATTTTAAATTTTTAAAAATTTG‑‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTa                                                              <  1:1251490/116‑1 (MQ=255)
                                                                  tAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTTTAAAATTTAAATATAAAtttt                                                               >  1:2047099/1‑128 (MQ=255)
                                                                  tAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTTTATAATTGAAATATAAATTTTattt                                                           >  1:169576/1‑125 (MQ=255)
                                                                   atttaataaataaaaataTTATTTTTTTTTATATTTTTTTTATATTTAAATTTTTAAAAAAATAATTTTAAATTGTAAAAAAAACC‑‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAgg                                                            <  1:1973878/118‑1 (MQ=255)
                                                                   aGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTTTTAATTTAAATAAAAATTTTatc                                                            >  1:1538003/1‑126 (MQ=255)
                                                                   aGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTAAAATGAAAAAATAAATTTTattt                                                           >  2:2337505/1‑125 (MQ=255)
                                                                    gTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTTTATGGAATTTTAATTTTTTGGttt                                                         >  2:1976328/1‑126 (MQ=255)
                                                                         aaactaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTAATAATGGAAATATAATTTTATGGTAttttt                                                     >  1:1216655/1‑127 (MQ=255)
                                                                           actaatactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTATTTTTTTTTTTTATTTATAATGTAAAAAAAAAATTTAGGTAattttt                                                    >  1:2171368/1‑126 (MQ=255)
                                                                            ataaaaattTTATTTTTTTTTTTTTTTTTTTTTAATTCAAATTTTTAAAAAATTAATTTTAAATTTTAAAAAATA‑‑‑‑TGTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTtgttgt                                                 <  2:639590/127‑1 (MQ=255)
                                                                             taaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTATATTGAAAAAATAAATTTAATGTtttttttttt                                                 >  1:960620/1‑126 (MQ=255)
                                                                              aaaaataTTATTTATTTTGATATTTTTTTTATTTTCAAATTTTTAAAAAATTAAATTTAACTTTTTAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCt                                               <  1:581749/128‑1 (MQ=255)
                                                                                aactaTTTACTTTTTAGTTATTTTTTGTTTTTGTAAATTTTTAAAAAATTAATTTGAAATTGTAAAAAAAAGC‑‑‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTaa                                             <  1:821860/128‑1 (MQ=255)
                                                                                aactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTTTATAATGCAAATATAAAATTATTGTATTTTTTTTTATaa                                             >  2:178938/1‑128 (MQ=255)
                                                                                aaatattatCTTTTTAGATATTTTTTTTATATTCAAAATTTTAAAAAAATAATTTTAACTTTTAAAAAATAGC‑‑GTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTa                                              <  1:2358464/125‑1 (MQ=255)
                                                                                 actaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTTATATTGATAATATAATTTTTATGTTTTTTGTTGTTTa                                              >  2:1581915/1‑128 (MQ=255)
                                                                                  aaattattTTTTTATTAAATTTTTGTTTAATCAAATTTTTAAAAAATAAAATTTTAATTTTTAAAAAAATG‑‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAg                                            <  1:267738/126‑1 (MQ=255)
                                                                                   tattatCTTTTTAGATATTTTTTGTATGTGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTTTATAATGAAAATAAAATTTTTATTTATTtttttgattatttt                                          >  2:1242423/1‑120 (MQ=255)
                                                                                    tttatttttttttttatttttttttTTTTTAAATTTTTTAAAAATTTATTTTTAATTTTTAAAAATAT‑‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTa                                          <  2:12999/127‑1 (MQ=255)
                                                                                    attatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTATTAATTTTGTTTTATAATTAt                                         >  1:479659/1‑128 (MQ=255)
                                                                                     ttatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTAAATGAAATTATAAATTTAAGTAATttttttatatatatatc                                        >  2:2083407/1‑117 (MQ=255)
                                                                                     ttatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTAATATTTAAATATTAATTTAAGTTATttttttttatatttata                                        >  2:262718/1‑117 (MQ=255)
                                                                                      tatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTATTTATTTTTTTTTCTAATTATc                                        >  1:2015600/1‑128 (MQ=255)
                                                                                        tCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTTTATATTGTAAATTTAAATTTTAGGTATTTTGTTTTATAATTATCTaa                                     >  1:2389237/1‑128 (MQ=255)
                                                                                           ttttAGAATTTTTTTGTATTTGCAAATTTTTAAAAAAAAAAATTTAAATTGTAAAAAATA‑‑‑‑TGTTTTTTTTTATTTTTTTATAATGGAAATATAAATTTAAGGTATTTTGTTGTCTAAGTATCTaaaaa                                  <  2:1371478/128‑1 (MQ=255)
                                                                                             ttttttatTTTTTTTATATTCAAATTTTTAAAAAATTTATTTTAAATTTTAAAAAATA‑‑‑‑TATTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTaaaaaaa                                <  1:1729017/123‑1 (MQ=255)
                                                                                             ttAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑‑‑TTTTTTTTTTTTTTTATAATTGAAATATAAATTTTAGGTATTTTTTTGTCTAATTATATAAAAAAATa                              >  1:2205142/1‑128 (MQ=255)
                                                                                             ttAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAG‑‑ATTTTTTTTTTTTTTTTTATAATTCAAATATAAATTTTTAGTTTTATTGTGTCTAAGTAtgatataaaa                                >  2:1089331/1‑119 (MQ=255)
                                                                                                  tatTTTTTTTTTTTTTAAATTTTTTTAAAATTAATTTTAAATTTTTAAAAATAT‑‑TTTTTTTTTTTTTTTTTTTATAATTGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGa                            <  2:1463185/128‑1 (MQ=255)
                                                                                                    ttttttGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTATATTGTAAATTTAAATTTTATTTTTTTTTTTGTATATTTAAGTAAAAAAAttaaaa                          >  1:2451369/1‑123 (MQ=255)
                                                                                                      ttttGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTATGTATTTTTTTTTATAATTATCTAAAAAAATAaaaatg                        >  1:1913216/1‑126 (MQ=255)
                                                                                                       ttttTATATTCAATATTTTAAAAAATTAATTTTTTATTTTAAATAAATT‑‑GTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGa                       <  1:2299555/128‑1 (MQ=255)
                                                                                                       tttGTATATGCAAATTTTTAAAAAATTAATTTGAAAATTTAAAAAAAATC‑‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGa                       <  1:1899204/128‑1 (MQ=255)
                                                                                                        tttatatttaaaaTTTTTAAAAATTTATTTTTAATTTTTAAAAAAATC‑‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGaaa                     <  2:314653/118‑1 (MQ=255)
                                                                                                         tGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTATAATGAAAATATAAATTTTATTTATTTTGTTGAATAATTATAAAAAAAAATAAAAAGTaaa                     >  2:2035410/1‑128 (MQ=255)
                                                                                                          ttttattaaaaTTTTTTAAAAATTTATTTTTAATTTTAAAAAATATA‑‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGaaa                     <  2:239941/120‑1 (MQ=255)
                                                                                                           ttaattgaaaTTTTTAAAAAAATAATTTTAAAATGTAAAAAAAATC‑‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGaaaaa                   <  1:300946/121‑1 (MQ=255)
                                                                                                            ttatttaaaTTTTTAAAAAAATAATTTTAAATTTTAAAAAATA‑‑‑‑TATTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAat                 <  1:1326386/122‑1 (MQ=255)
                                                                                                             tatttaaaTTTTTAAAAAATTAATTTTAACTTTTTAAAAATATC‑‑TTTTTTTTTTTTTTTTTTATAATGGAAATGTAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAat                 <  1:654110/123‑1 (MQ=255)
                                                                                                             tatGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTATAAATTAAAAAAAAATTTTAAGTATTTTTTTTTATAAATAAAAAAAAAAATAAAAATTaaaaaaa                 >  2:361376/1‑127 (MQ=255)
                                                                                                             tatGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTAAAATTTAAATATAAATTTTATGTTTTTTTTTTTATAATTATATAAAAAAATAAAAAGAAAAAAat                 >  2:597824/1‑128 (MQ=255)
                                                                                                               tttaaaTTTTTAAAAAATTTATTTTTAATTTTTAAAAATTTT‑‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAata                <  2:2291961/125‑1 (MQ=255)
                                                                                                               tGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTATAATGTAATTATAAATTTTAGTTTTTTTGTTTAATAAGTATATAAAAAAATAGAAAGTAAATAatat               >  1:2234283/1‑128 (MQ=255)
                                                                                                                  aaaTTTTTAAAAAATTTATTTTAAATTGTTAAAAAT‑‑T‑‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGtt           <  2:840608/128‑1 (MQ=255)
                                                                                                                  aaaTTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTT‑‑TAATTGAAATATAAATTTTAGTTTTTTTTTTTTATAATTAACTAAAAAAATATAAATGAAAAAATATtttt           >  1:2113659/1‑128 (MQ=255)
                                                                                                                  aaaTTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑‑TTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTAGGTATTTTTTTTTATAATTTTATAAAAAAATAAAAAGTAAAAAATATTTTTa          >  1:1126906/1‑128 (MQ=255)
                                                                                                                   aaTTTTTAAAAAATTATATTTTACATGTAAAAAATA‑‑‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGGCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTa          <  2:2453331/128‑1 (MQ=255)
                                                                                                                         ttaaaaaaTTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTATTTATTTTTTTTTATAATTATATAAAAAAATAAAAAGAAAAAAATATTTTTtaaat      >  1:908434/3‑127 (MQ=255)
                                                                                                                          taaaaaaTTAATTTTAAATTTTAAAAAATAT‑‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGt     <  2:810229/127‑1 (MQ=255)
                                                                                                                              |                                                                                                                                  
CTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTAAAAGAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAAT  >  CP016816/165097‑165349

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: