BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP016816	165,218	A→T	intergenic (+182/‑360)	JCVSYN2_00665 → / → JCVSYN2_00670	tRNA N6‑adenosine(37)‑N6‑threonylcarbamoyltransferase complex dimerization subunit TsaB/tRNA‑Ser

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP016816	165,218	0	A	T	96.8%	2844.5 / 2.1	1027	intergenic (+182/‑360)	JCVSYN2_00665/JCVSYN2_00670	tRNA N6‑adenosine(37)‑N6‑threonylcarbamoyltransferase complex dimerization subunit TsaB/tRNA‑Ser
Reads supporting (aligned to +/- strand): ref base A (8/25); new base T (669/325); total (677/350)
Fisher's exact test for biased strand distribution p-value = 7.97e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1073 total aligned reads displayed.

TTTACTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTAAAAGAAT‑TAATTTGAACTTGTAAAAAATA‑GC‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAA  >  CP016816/165093‑165343

tttACTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTtaa                                                                                                                                <  1:1111397/128‑1 (MQ=255)

tttACTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTtaa                                                                                                                                <  1:400051/128‑1 (MQ=255)

tttACTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTACATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTtaa                                                                                                                                >  1:787854/1‑128 (MQ=255)

  tACTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGAATATGCAAA‑TTTTtaaaa                                                                                                                              <  2:1293889/128‑2 (MQ=255)

   aCTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTtaaaaa                                                                                                                             >  2:1473702/1‑126 (MQ=255)

   aCTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTtaaaaa                                                                                                                             <  1:2365662/128‑3 (MQ=255)

     tttAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGAAAA‑TTTTTAAAAaat                                                                                                                           >  2:1442195/1‑128 (MQ=255)

      ttAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAaat‑t                                                                                                                         >  1:902872/1‑128 (MQ=255)

       tAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAGCTATTATCTTTTTAGATAATTTTTGTATATGCAAA‑TTTTTAAAAaat‑ta                                                                                                                        >  2:819078/1‑128 (MQ=255)

       tAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAaat‑ta                                                                                                                        <  1:2121239/128‑1 (MQ=255)

       tAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAaat‑ta                                                                                                                        >  2:1647803/1‑128 (MQ=255)

       tAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTGTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAaat‑ta                                                                                                                        >  2:1206100/1‑128 (MQ=255)

         aTTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TATTTAAAAaat‑taat                                                                                                                      >  2:2164517/1‑128 (MQ=255)

          ttttATTAATTTACTAGTTGATAATATTTAGTTTAATTAATTTATCGCAATTGTATGAATTAGTAAGTAACTAAAACTTTTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAaat‑taatt                                                                                                                     <  2:1817130/125‑1 (MQ=255)

           tGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAAT‑TAAttt                                                                                                                    <  1:2425322/128‑1 (MQ=255)

           tGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAAT‑TAAttt                                                                                                                    <  2:1969909/128‑1 (MQ=255)

           tGTATTACTTTACTAGTTGATAATATTTAGTTTCAATAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAAT‑TAAttt                                                                                                                    <  1:1003197/128‑1 (MQ=255)

            gTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAAT‑TAATTTg                                                                                                                   <  1:2076568/128‑1 (MQ=255)

            gTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAAT‑TAATTTg                                                                                                                   >  1:2055290/1‑128 (MQ=255)

             tATTACTTTACTAGTTTATAATATTTAGATTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAAT‑TAATTTGa                                                                                                                  <  2:1269397/128‑1 (MQ=255)

             tATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATAGCACTTGTATGAATTATTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTAAAAAAAT‑TAATTTGa                                                                                                                  >  1:610322/1‑128 (MQ=255)

              aatacattacaAGTCGATAATATATAGATTCATAAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAAT‑TAATTTGaa                                                                                                                 <  2:636335/126‑1 (MQ=255)

              aTTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAAT‑TAATTTGaa                                                                                                                 >  2:95911/1‑128 (MQ=255)

                 actttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAAT‑TAATTTGAACtt                                                                                                              >  1:239345/1‑128 (MQ=255)

                     tactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAAT‑TAATTTGAACTTGTaa                                                                                                          <  1:1378304/128‑1 (MQ=255)

                       ctAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAAT‑TAATTTGAAATTGTaaaa                                                                                                        <  1:1205687/128‑1 (MQ=255)

                        tAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAAT‑TAATTTTAACTTGTaaaaa                                                                                                       >  1:52660/1‑128 (MQ=255)

                        tAGTTGATAATATTTAGTTTCATTAATTTATCGAACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAAT‑TAATTTGAACTTGTaaaaa                                                                                                       <  1:1896723/128‑1 (MQ=255)

                          gTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAAT‑TAATTTGAACTTGTAAAAAAt                                                                                                     >  2:765835/1‑128 (MQ=255)

                           ttGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAAT‑TAATTTGAACTTGTAAAAAATa                                                                                                    >  2:2061358/1‑128 (MQ=255)

                               taataTTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATTTGCAAA‑TTTTTAAAAAAT‑TAATTTTAACTTGTAAAAAATA‑TC‑tt                                                                                              >  2:1550899/1‑128 (MQ=255)

                                aagatTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGAGATTTTTTGTATATGCAAA‑TTTTTAATTAAT‑TAATTTGAACTTGTAATAAATA‑GC‑tgt                                                                                             >  2:2129334/4‑126 (MQ=255)

                                 atatTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAAT‑TAATTTGAACTTGTAAAAAATA‑GC‑tttt                                                                                            >  1:2329069/1‑128 (MQ=255)

                                    tttATTTTAATTAATTTTTAGCAATTTTTATAATTATTAATAAAATAAAAAAATTATCTTTTTTTTTATTTTTTTTATTTTCAAA‑TTTTTAAAAAAT‑TAATTTTAACTTTTAAAAAATA‑GC‑ttttttt                                                                                         <  2:53914/128‑1 (MQ=255)

                                      tAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAAT‑TAATTTGAACTTGTAAAAAATA‑GC‑ttttttttt                                                                                       >  1:720406/1‑128 (MQ=255)

                                         tttCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAAT‑TAATTTGAACTTGTAAAAAATA‑GC‑tttttttttttt                                                                                    >  1:1431684/1‑128 (MQ=255)

                                         tttCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAAT‑TAATTTCAACTTGTAAAAAATA‑GC‑ttttattttttt                                                                                    >  2:104199/1‑128 (MQ=255)

                                           tCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAAT‑TAATTTGAACTTGTAAAAAATA‑GC‑tttttttttttttt                                                                                  >  1:1728929/1‑128 (MQ=255)

                                           tCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAAT‑TAATTTGAACTTGTAAAAAATA‑GC‑tttttttttttttt                                                                                  >  1:1582257/1‑128 (MQ=255)

                                           tCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAAT‑TAATTTGAACTAGTAAAAAATA‑GC‑tttttttttttttt                                                                                  >  2:510832/1‑128 (MQ=255)

                                           tCATTAATATATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATAATTTTTGTATATGCAAA‑TTATTAAAAAAT‑TAATTTGCAATTTTAAATAATA‑TC‑ttttaatattattt                                                                                  >  2:250524/1‑118 (MQ=255)

                                            cATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAAT‑TAATTTGAACTTGTAAAAAATA‑GC‑ttttttttttttttt                                                                                 >  2:882967/1‑128 (MQ=255)

                                             aTTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAAT‑TAATTTGAACTTGTAAAAAATA‑GC‑tttttttttttttttt                                                                                >  1:1172467/1‑128 (MQ=255)

                                                  tttATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAAT‑TAATTTGAACTTGTAAAAAATA‑GC‑ttttttttttttttttttttt                                                                           >  2:306879/1‑126 (MQ=255)

                                                    tATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAAT‑TAATTTGAACTTGTAAAAAATA‑GC‑TTTTTTTTTTTTTTTTTTTATaa                                                                         >  2:353410/1‑128 (MQ=255)

                                                     aTCGCACTTGTATTTATTATTAATAAACTAAAAATATTATGTTTTTAGAAATTTTTTGTATATTCAAA‑TTTTTAAAAAAT‑TAATTTGAACTTTTAAAAAATA‑GC‑‑TTTTTTTTTTTTTTTTTTATAATg                                                                       <  2:1908954/128‑1 (MQ=255)

                                                     aTCGCAATTGTATGAATTATTAATAAACTAAAAATATTATTTTTTTATTTATTTTTTTTATATGCAAA‑TTTTTAAAAAAT‑TAATTTGAACTTTTAAAAAATA‑TC‑tttttttttttttttatataatgg                                                                        <  2:842087/128‑10 (MQ=255)

                                                      tCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAAT‑TAATTTGAACTTGTAATACATA‑GC‑tttttttttttttttttattaattc                                                                       >  2:681638/1‑120 (MQ=255)

                                                       ctaaattttattaattattaatTAAATAAAAATATTATCTTTTTATATATTTTTTTTATATGCAAA‑TTTTTAAAAAAT‑TAATTTTAAATTTTAAAAAATA‑TC‑‑TTTTTTTTTTTTTTTTTTATAATGGa                                                                     <  1:432965/120‑1 (MQ=255)

                                                        gCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAAT‑TAATTTGAACTTGTAAAAAATA‑GC‑TTTTTTTTTTTTTTTTTatattttaaa                                                                     >  1:1961168/1‑122 (MQ=255)

                                                            ttGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAAT‑TAATTTGAACTTGTAAAAAATA‑TC‑TTTTTTTTTTTTTTTTTTT‑TAATTGAAatta                                                                >  1:1060446/1‑126 (MQ=255)

                                                            ttGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAAT‑TAATTTGAACTTGTAAAAAATA‑GC‑TTTTTTTTTTTTTTTTTTTATAAttaaataa                                                                 >  1:683244/1‑121 (MQ=255)

                                                             tGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAAT‑TAATTTGAACTTGTAAAAAATA‑GC‑TTTTTTTTTTTTTTTTtataattaaaaaataa                                                                >  2:1763320/1‑116 (MQ=255)

                                                                 tGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAAT‑TAATTTGAACTTGTAAAAAATA‑GC‑‑TTTTTTTTTTTTTTTTTTA‑AATTTAAATATAAAttt                                                          >  1:1537938/1‑128 (MQ=255)

                                                                  gAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAAT‑TAATTTGAACTTGTAAAAAATA‑GC‑‑TTTTTTTTTTTTTTTTTTATAATTTAAAAATAAAttt                                                          >  1:1473320/1‑128 (MQ=255)

                                                                   aaTTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAAT‑TAATTTGAACTTGTAAAAAATA‑GCTTTTTTTTTTTTTTTTTTTTATAATTTAAATAAAAAtt                                                           >  1:1155242/1‑128 (MQ=255)

                                                                    ttttttaataaAATAAAAATATTTTATTTTTATATATTTTTTTTAAATTAAAA‑TTTTTAAAAAAT‑TAATTTTAAATTTTAAAAAATA‑‑‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAg                                                       <  1:1788069/123‑1 (MQ=255)

                                                                     ttAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAAT‑TAATTTGAACTTGTAAAAAATA‑GC‑TTTTTTTTTTTTTTTTATTAT‑‑T‑GAAATATAAATTTTAgtt                                                     >  1:2279917/1‑126 (MQ=255)

                                                                      tAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAAT‑TAATTTGAACTTGTAAAAAATA‑GC‑TTTTTTTTTTTTTTTTTTATAAATATAAATAAAAAtttatg                                                       >  1:2459166/1‑125 (MQ=255)

                                                                       tttaataaAATAAATATATTTTTTTTTTAGATATTTTTTTTAAATGCAAA‑TTTTTAAAAAAT‑TAATTTGAAATTTTAAAAAAAA‑TC‑‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGt                                                     <  2:1596763/126‑1 (MQ=255)

                                                                        gttaagaattaaaacttTTTTCTTTTTAGTTATTTTTTTTTTATTTAAA‑TTTTTAAATAAA‑TTATTTCTAATTGTAAAAAATA‑GC‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGt                                                     <  2:449982/119‑1 (MQ=255)

                                                                             aaactaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAAT‑TAATTTGAACTTGTAAAAAATA‑GC‑‑TTTTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTATTTAttttt                                               >  1:839666/1‑127 (MQ=255)

                                                                              aactaaaactaTTATCTTTTTAGATATTTTTTGTATGTGCAAA‑TTTTTAAAAAAT‑TAATTTGAACTTGTAAAAAATA‑GC‑TTTTTTTTTTTTTTTTTTT‑TAATGTTAATATAAATTTTTGGTAtttttt                                              >  2:493765/1‑126 (MQ=255)

                                                                              aactaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAAT‑TAATTTGAACTTGTAAAAAATA‑GC‑‑‑TTTTTTTTTTTTTTTTTATAATTAAAATTTAAATTTTATGAATtttttt                                             >  1:1248621/1‑128 (MQ=255)

                                                                              aactaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAAT‑TAATTTGAACTTGTAAAAAATA‑GC‑‑‑TTTTTTTTTTTTTTTTTA‑AAT‑GAAATATAAATTTTATGTtttttttttt                                           >  2:2382358/1‑126 (MQ=255)

                                                                               actaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAAT‑TAATTTGAACTTGTAAAAAATA‑GC‑‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGTTATtttttt                                             >  2:1689390/1‑128 (MQ=255)

                                                                                 taaaactaTGATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAAT‑TAATTTGAACTTGAAAAAAATA‑GC‑‑TTTTTTTTTTTTTTTTTGAAAATGGAAAAATAAATTTTAGTAATTtttttga                                           >  2:591537/1‑127 (MQ=255)

                                                                                  aaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAAT‑TAATTTGAACTTGTAAAAAATA‑GC‑TTTTTTTTTTTTTTTTTTT‑TAATGTAAATATAAATTTTAGGTATtttttttta                                          >  1:818805/1‑125 (MQ=255)

                                                                                   aaactaTTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAAT‑TAATTTGAACTTGTAAAAAATA‑GC‑‑TTTTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTATTTATttttttttat                                         >  1:963980/1‑124 (MQ=255)

                                                                                      aaattattTTTTTATTAAATTTTTGTTTAATCAAA‑TTTTTAAAAAATAAAATTTTAATTTTTAAAAAAAT‑G‑‑‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAg                                      <  1:267738/126‑1 (MQ=255)

                                                                                       tattatCTTTTTAGATATTTTTTGTATGTGCAAA‑TTTTTAAAAAAT‑TAATTTGAACTTGTAAAAAATA‑GC‑‑‑TTTTTTTTTTTTTTTTTATAATGAAAATAAAATTTTTATTTATTtttttgattatttt                                    >  2:1242423/1‑120 (MQ=255)

                                                                                       tattatCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAAT‑TAATTTGAACTTGTAAAAAATA‑GC‑‑‑‑TTTTTTTTTTTTTTTTATAATTAAAATATAAATATTATTAATTTTTTTTTATAATTAt                                   >  1:33850/1‑128 (MQ=255)

                                                                                        attatCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAAT‑TAATTTGAACTTGTAAAAAATA‑GC‑‑‑TTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTATTAATTTTGTTTTATAATTAt                                   >  1:479659/1‑128 (MQ=255)

                                                                                         ttatCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAAT‑TAATTTGAACTTGTAAAAAATA‑GC‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGTTAATATTTTTTATAAGTa                                    >  1:874414/1‑128 (MQ=255)

                                                                                          tatCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAAT‑TAATTTGAACTTGTAAAAAATA‑GC‑‑TTTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTATTTATTTTTTTTTCTAATTATc                                  >  1:2015600/1‑128 (MQ=255)

                                                                                           atCTTTTTTTATATTTTTTTTATATTAAAA‑TTTTTAAAAAAA‑TATTTTTTAATTTTTAAAAATA‑‑T‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCt                                 <  2:1869126/128‑1 (MQ=255)

                                                                                               tttttttttttttttttttttGTAAATTTTTTAAAAATT‑TATTTTTAATTTTTAAAAAATT‑G‑‑‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTaaaa                             <  2:1104567/119‑1 (MQ=255)

                                                                                                 ttAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAAT‑TAATTTGAACTTGTAAAAAATA‑GC‑‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAATATTAGTTATTTTTTTTTATAAGTATCaaaaaaaa                          >  2:1895303/1‑128 (MQ=255)

                                                                                                      tatTTTTTTTATTTTGAAA‑TTTTTTAAAAAT‑TTATTTTAACTTTTTAAAAATT‑TC‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAg                       <  2:2243951/128‑1 (MQ=255)

                                                                                                       tttttttttttATTCAAA‑TTTTTAAAAAAT‑TAATTTTTAATTTTAAAAAATA‑‑‑‑‑TATTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAg                   <  2:1357026/127‑1 (MQ=255)

                                                                                                       atTTTTTGTATATGCAAA‑TTTTTAAAAAAT‑TAATTTGAACTTGTAAAAAATA‑GC‑‑TTTTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTATTTATTTTTTTTTCTAAGTATATAAAAAAATATaa                     >  1:1326245/1‑128 (MQ=255)

                                                                                                        ttttttttATTTTCAAA‑TTTTTAAAAAAT‑TTATTTTTAATTTTTAAAAATA‑‑T‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGaaa                    <  2:1401355/128‑1 (MQ=255)

                                                                                                         tttttGTATATGCAAA‑TTTTTAAAAAAT‑TAATTTGAACTTGTAAAAAATA‑GC‑‑‑TTTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTAGTTATTTTTTTGTCTAATTATATAAAAAAATAGAAAgt                  >  1:1646682/1‑127 (MQ=255)

                                                                                                          ttttGTATATGCAAA‑TTTTTAAAAAAT‑TAATTTGAACTTGTAAAAAATA‑GC‑‑TTTTTTTTTTTTTTATTTATAATTTAAATAAAAATTTTAGTTATTTTTTTTTATAATTATATAAAAAAATTGaaatg                  >  1:2245413/1‑126 (MQ=255)

                                                                                                          ttttGTATATGCAAA‑TTTTTAAAAAAT‑TAATTTGAACTTGTAAAAAATA‑GC‑‑‑TTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTAGGTTTTTTGTTTTATAAGTATATaaaaaaaattaaagta                 >  2:387034/1‑119 (MQ=255)

                                                                                                            ttGTATATGCAAA‑TTTTTAAAAAAT‑TAATTTGAACTTGTAAAAAATA‑GC‑‑TTTTTTTTTTTTTTTTTTATAATTGAAATATAAATTTTAGGTTTTTTGTTTTATAAGTATCTAAAAAAATAAAAATGaa                >  1:1853969/1‑128 (MQ=255)

                                                                                                             tGTATATGCAAA‑TTTTTAAAAAAT‑TAATTTGAACTTGTAAAAAATA‑GC‑‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTTTTTTGTTGTGTAAGTATCTAAAAAAATAGAATGTaaa               >  2:2278137/1‑128 (MQ=255)

                                                                                                               ttaattgaaa‑TTTTTAAAAAAA‑TAATTTTAAAATGTAAAAAAAA‑TC‑‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGaaaaa             <  1:300946/121‑1 (MQ=255)

                                                                                                                atatTCAAT‑TTTTTAAAAAAA‑TAAATTTAACTTTTTAAAAATA‑TG‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGaaaaa             <  1:2208454/128‑1 (MQ=255)

                                                                                                                 tatttaaa‑TTTTTAAAAAAT‑TAATTTTAACTTTTTAAAAATA‑TC‑‑TTTTTTTTTTTTTTTTTTATAATGGAAATGTAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAat           <  1:654110/123‑1 (MQ=255)

                                                                                                                 tatTCAAA‑TTTTTAAAAAAT‑TAATTTGAAATTTTAAAAAATA‑‑T‑GTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAat           <  2:2022576/128‑1 (MQ=255)

                                                                                                                  atGCAAA‑TTTTTAAAAAAT‑TAATTTGAACTTGTAAAAAATA‑GA‑TTTTTTTTTTTTTTTTTTTTTAATTTAAATATTAATTTTATTTATATTTTTATATAAGTATATAAAAAAATTTAAAAGAAAAAat           >  1:579750/1‑128 (MQ=255)

                                                                                                                    ttaaa‑TTTTTAAAAAAT‑TAATTTTTTATTTTAAAAAATATTT‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAata          <  2:1155242/126‑1 (MQ=255)

                                                                                                                    gCAAA‑TTTTTAAAAAAT‑TAATTTGAACTTGTAAAAAATA‑GC‑‑TTTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTAGGTATTTTGTTGTATAATTATATAAAAAAATTTAAAGGAAAAAATAtt        >  1:168034/1‑128 (MQ=255)

                                                                                                                     cAAA‑TTTTTAAAAAAT‑TAATTTGAACTTGTAAAAAATA‑GC‑TTTTTTTTTTTTTTTTTTTATAATTAAAAAATAAATTTAATTTATTTTTTTGTATAATAATATAAAAAAAAAAAAATAAAAAAATAtt        >  1:2173852/1‑128 (MQ=255)

                                                                                                                     cAAA‑TTTTTAAAAAAT‑TAATTTGAACTTGTAAAAAATA‑GC‑TTTTTTTTTTTTTTTTTTTATAATGGAAATAAAAATTTTAGGTATTTTTTTTTATAAGTATATAAAAAAATAGAAAGTAAAAAATAtt        >  1:1913140/1‑128 (MQ=255)

                                                                                                                      aaa‑TTTTTAAAAAAT‑TAATTTGAACTTGTAAAAAATA‑GC‑‑TTTTTTTTTTTTTTTTTTATAATGTAAAAAAAAATTTTAGTTATTTTGTTGAGTAAGTATAAAAAAAAATAAAAAGTAAAAAATAtttt      >  1:1297375/1‑126 (MQ=255)

                                                                                                                        a‑TTTTAAAAAATT‑TAATTTAAAATT‑TTAAAAATA‑TC‑‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTaa   <  1:753655/128‑1 (MQ=255)

                                                                                                                        a‑TTTTAAAAAAAT‑AAATTT‑ATCATTTAAAAAA‑A‑TT‑TTATTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTaa   <  2:2422501/128‑1 (MQ=255)

                                                                                                                          ttttTAAAAAAT‑TAATTTGAACTTGTAAAAAATA‑GC‑‑‑TTTTTTTTTTTTTTTTTATATTGAAAATTAAAATTTTAGTTTTTTTTTTGTAAAATTAAATAAAAAAAAAAAAAGTAAAAAATATTTTTaaa  >  2:2312878/1‑128 (MQ=255)

TTTACTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTAAAAGAAT‑TAATTTGAACTTGTAAAAAATA‑GC‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAA  >  CP016816/165093‑165343

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`