Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 484,791 T→A intergenic (‑100/+71) JCVSYN2_02220 ← / ← JCVSYN2_02225 hypothetical protein/50S ribosomal protein L7/L12

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816484,7910TA96.8% 2750.3 / 20.5 971intergenic (‑100/+71)JCVSYN2_02220/JCVSYN2_02225hypothetical protein/50S ribosomal protein L7/L12
Reads supporting (aligned to +/- strand):  ref base T (16/15);  new base A (456/484);  total (472/499)
Fisher's exact test for biased strand distribution p-value = 8.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1003 total aligned reads displayed.

ATTATTTAAAAAATAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTTAAAAAAAAAACAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATTTCT  >  CP016816/484665‑484913
                                                                                                                              |                                                                                                                           
attattTAAAAAATAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTTa                                                                                                                            <  2:1938020/128‑1 (MQ=255)
       aaaaaaTAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAatataaaa                                                                                                                    >  2:357925/1‑128 (MQ=255)
        taaaaTAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAatataaaaa                                                                                                                   <  2:846016/127‑1 (MQ=255)
          aaaTAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAatataaaaatc                                                                                                                 >  1:1886894/1‑126 (MQ=255)
            aTAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAATCaa                                                                                                               >  1:76083/1‑128 (MQ=255)
            aTAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCaa                                                                                                               >  2:1437373/1‑128 (MQ=255)
              aGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACAAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTAAACTCGATAATTTAATTATATTATTTATTAAGATATAAAGCAAtt                                                                                                             >  1:647900/1‑128 (MQ=255)
              aGTCAAAACTCATAGATTTAATCCTTTTATAGTTGAACAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAAtt                                                                                                             <  2:1025087/128‑1 (MQ=255)
                tCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTaa                                                                                                           >  1:2242549/1‑128 (MQ=255)
                     aCTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAacaca                                                                                                      >  1:944153/1‑128 (MQ=255)
                          tAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATATTTTTTTAATATAAAAAGCAATTAAACACACaaaa                                                                                                 >  1:1057663/1‑128 (MQ=255)
                           aGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAATCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACaaaaa                                                                                                <  2:2264090/128‑1 (MQ=255)
                            gATTTAATGCTTTTATAGTTTAACAAAAAATAATATAGAGCATAGCAGAAGATCTTAACAACTAATCTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAAc                                                                                               <  1:1513036/128‑1 (MQ=255)
                            gATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGCGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAAc                                                                                               <  2:1284926/128‑1 (MQ=255)
                              tttAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCAATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTc                                                                                             >  1:1215709/1‑128 (MQ=255)
                                tAATCCTTTTATATTTTAACAAAAAATAAGATAGATCATAGCAGAAGATCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACAGACAAAAACTCAc                                                                                           <  1:1642791/128‑1 (MQ=255)
                                 aaTCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTAAcc                                                                                          >  2:118153/1‑128 (MQ=255)
                                  aTCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCa                                                                                                                <  1:905817/105‑1 (MQ=255)
                                    ccTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCAt                                                                                       <  1:673076/128‑1 (MQ=255)
                                    ccTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAAATAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAccata                                                                                       <  1:573307/128‑4 (MQ=255)
                                     cTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCTATTATTTAATTATATTTTTTTTTATTATAAAAATCAATTTAACACACAAAAACTCAccactt                                                                                      >  2:1547697/1‑124 (MQ=255)
                                     cTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCAtt                                                                                      <  2:1397059/128‑1 (MQ=255)
                                     cTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTa                                                                                     <  2:705590/128‑1 (MQ=255)
                                       tttATAGTTGACCAAAAGATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGAGTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTAt                                                                                    >  2:1233501/1‑128 (MQ=255)
                                       tttATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTAt                                                                                    >  2:877153/1‑128 (MQ=255)
                                       attctaGTTGAACAAAAAATAAGATAGAGCATAGAAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTAt                                                                                    <  1:1003894/124‑1 (MQ=255)
                                         tataGTTGAACAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATgg                                                                                  <  2:586304/128‑1 (MQ=255)
                                          atatTTCACCAAAAAATAAGAAAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAccataatgggt                                                                                 <  1:1558727/128‑10 (MQ=255)
                                          ataGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGt                                                                                 >  2:2007440/1‑128 (MQ=255)
                                           taGTTCACCAAAAAATAAGATACAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTg                                                                                <  2:1505765/128‑1 (MQ=255)
                                            aGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAACCTCACCCATTATGGTGa                                                                               <  2:1463067/128‑1 (MQ=255)
                                            aGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGa                                                                               <  1:1935599/128‑1 (MQ=255)
                                                 aCCAAAAAATAAGATAGAGCATAGGAGAAGCTCTTAACAACTAAACTACACTCGATTTGTTAAGTATATTTTTTTTTAATATACAAAGAAATTAAACAAACAAAAACACACCAAatatttgtctttta                                                                          >  2:1596864/1‑118 (MQ=255)
                                                  ccAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTaa                                                                         <  1:355980/128‑1 (MQ=255)
                                                    aaaaaaTATGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGtttttgt                                                                       <  2:417790/128‑5 (MQ=255)
                                                    aaaaaaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTaaaa                                                                       <  1:1846001/128‑1 (MQ=255)
                                                     taaaaTAAGATACAGCATAGCAGAAGATCTTAACAACTAAACAACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTaaaaa                                                                      <  2:805097/127‑1 (MQ=255)
                                                     aaaaaTAATCTAGATCATAGCAGAAGCTCTAAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTaaaaa                                                                      <  2:2071657/128‑1 (MQ=255)
                                                     aaaaaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTaaaaa                                                                      <  1:479383/128‑1 (MQ=255)
                                                      aaaaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATAAAACACACAAAAACTAACACATTATGGTGAGTTTAAAAat                                                                     >  1:991717/1‑128 (MQ=255)
                                                            aGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCa                                                               <  1:2332134/128‑1 (MQ=255)
                                                              aTAGATCATATCAGAATCTCTTAAAAACTAAACTAAACTCTATTATTTATTTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAACCATTATGGTGAGTTTAAAAATTATTCaaa                                                             <  1:1076448/128‑1 (MQ=255)
                                                              aTAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCaaa                                                             >  2:2149629/1‑128 (MQ=255)
                                                               tAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTATTTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCaaac                                                            >  2:275546/1‑127 (MQ=255)
                                                               tAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGAATATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCaaaa                                                            <  1:289786/128‑1 (MQ=255)
                                                                agagCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCaaaaa                                                           <  2:517140/128‑1 (MQ=255)
                                                                 gagCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAAt                                                          >  1:1115632/1‑128 (MQ=255)
                                                                  agCATAGCAGAAGCTCTTAACAACTAAACTGCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAAtt                                                         >  1:2181508/1‑128 (MQ=255)
                                                                  agCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAAtt                                                         >  2:786363/1‑128 (MQ=255)
                                                                      tAGCAGTATCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGat                                                     <  1:1289108/128‑1 (MQ=255)
                                                                      tAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAATACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGat                                                     <  1:88715/128‑1 (MQ=255)
                                                                      tAGCAGAAGCTCTTAACAACTAAACTACACTCGAATAATTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGat                                                     <  1:449700/128‑1 (MQ=255)
                                                                            aaGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAAATTGattattat                                               >  1:794679/1‑126 (MQ=255)
                                                                               ctctTAACAACTAAACTACCCTCGATTATTTAAGTATATTTTTTTTTTATAAAAAAAGCAATTAACCACACAAAAACTCACCCAATTTGGTGAGTTTAAAAAGTCTTCAAAAATTTGAGTATTTTaac                                            >  1:1592170/1‑127 (MQ=255)
                                                                                 ctTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATc                                          <  1:2010754/128‑1 (MQ=255)
                                                                                    aacaacTAAACTACCCTCGATTATTTAATTATAATTTATTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAAt                                       <  1:1163935/128‑1 (MQ=255)
                                                                                    aacaacTAAACTACACTAGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAAt                                       <  2:1123966/128‑1 (MQ=255)
                                                                                     acaacTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAAtt                                      <  2:745977/128‑1 (MQ=255)
                                                                                      caacTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGAGTATTTTAAATCAATTg                                     >  2:2191246/1‑128 (MQ=255)
                                                                                       aactaaactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGa                                    >  1:1480622/1‑128 (MQ=255)
                                                                                       aactaaactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGa                                    <  1:2450567/128‑1 (MQ=255)
                                                                                       aactaaactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGa                                    >  2:481076/1‑128 (MQ=255)
                                                                                         ctaaactaCACTAGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACACATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAg                                  <  2:1865068/128‑1 (MQ=255)
                                                                                           aaactaCACTCGAGTATTTAATTATATTATTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGGGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCt                                <  1:1778725/128‑1 (MQ=255)
                                                                                            aactaCACTAGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCtg                               <  2:24892/128‑2 (MQ=255)
                                                                                            aactaCACTAGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATAAATTGAAGCTc                               <  2:950458/128‑1 (MQ=255)
                                                                                            aactaCAATCGAATATTTAAATATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTc                               <  1:2295694/128‑1 (MQ=255)
                                                                                             actaCACTCGATTATATAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTcc                              <  1:2386623/128‑1 (MQ=255)
                                                                                             actaCACTAGATTATATAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTcc                              <  2:169588/128‑1 (MQ=255)
                                                                                              ctaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCa                             >  1:1329019/1‑128 (MQ=255)
                                                                                              ctaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCa                             >  2:2239788/1‑128 (MQ=255)
                                                                                               taCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCCCCCAGTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATGTAACATCACTTGAAGCTCCAg                            >  1:732027/1‑128 (MQ=255)
                                                                                                agattctcatatataATTATTTTATTTTTTAATATAAAAAGCAATTAAAAAAACAAAAACTAACAAATAATTGTTATTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGc                           <  2:174887/119‑1 (MQ=255)
                                                                                                  acTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTATAAATCAATTGAAGCTCCAGCtt                         >  1:415074/1‑128 (MQ=255)
                                                                                                  acTCGATAATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTAAAACATTATGTTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCtt                         <  1:137088/128‑1 (MQ=255)
                                                                                                    ttgATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTCAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCa                       <  2:1821085/126‑1 (MQ=255)
                                                                                                    tagATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCa                       <  2:608743/126‑1 (MQ=255)
                                                                                                     cGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCaa                      <  2:2434021/128‑1 (MQ=255)
                                                                                                      gATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACCCACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAAc                     >  2:54933/1‑128 (MQ=255)
                                                                                                      gATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAAc                     >  1:840971/1‑128 (MQ=255)
                                                                                                      gATTATATAATTATATTTTTTTTTAAAATAAAAACAAATTAAACACACAAAAACACACAAATTATGTTTATTTTAAAAATTATTAAAAAATTTAATAAATTTAAATAAATTGAAGATCCAGCTTCAAc                     >  2:2385914/1‑128 (MQ=255)
                                                                                                       attattTAATTATATTTTTTTTTAATATAAAAAGCAAATAAACACACAAAAACTCACCCATAATGGTGAGTTTAAAAATTATTAAAAAATATGATTATATTATATCAATTGAAGCTCCAGCTTCTACt                    >  1:1423925/1‑128 (MQ=255)
                                                                                                          attTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAAt                 >  1:545910/1‑128 (MQ=255)
                                                                                                             tAATTATATTTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCAATAAGGTGAGTTTAAAAATTATACAAAAATTTGATTATTTTAAATCAATTGAAACTCCAGCTTCAACTAAttt               >  2:1080272/1‑128 (MQ=255)
                                                                                                             tAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTa              <  1:2427530/128‑1 (MQ=255)
                                                                                                             tAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTa              >  2:1365454/1‑128 (MQ=255)
                                                                                                              aaTTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAGTTGAAGCTGGAGCTTCAATTGATTTaa             <  2:2328685/128‑2 (MQ=38)
                                                                                                              aaTTATATTTATATATAATATAAAAAGCAATTAAACACACCAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTGGAGCTTCAATTGATTTaa             <  1:1239561/128‑2 (MQ=255)
                                                                                                               aTTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGc            >  1:2070848/1‑128 (MQ=255)
                                                                                                                ttATATTTTTTTTTAATATAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCtt          >  2:630365/1‑128 (MQ=255)
                                                                                                                 tataTTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCtt          <  1:2122259/128‑1 (MQ=255)
                                                                                                                  atatTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCttt         >  1:169238/1‑128 (MQ=255)
                                                                                                                  atatTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCttt         >  1:513518/1‑128 (MQ=255)
                                                                                                                  atatTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCttt         >  2:1676834/1‑128 (MQ=255)
                                                                                                                   tatTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTc        <  2:1649845/128‑1 (MQ=255)
                                                                                                                    atTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCa       >  2:31206/1‑128 (MQ=255)
                                                                                                                      ttttttttaATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCAtt     >  2:914781/1‑128 (MQ=255)
                                                                                                                       tttttttaATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCAttt    <  1:857643/128‑1 (MQ=255)
                                                                                                                       tttttttaATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATAGAAGCTCCAGCTTCAACTAATTTAGCTTTCAttt    <  1:699503/128‑1 (MQ=255)
                                                                                                                         tttttaATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTAAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGATTTCATttct  >  1:1731219/1‑128 (MQ=255)
                                                                                                                              |                                                                                                                           
ATTATTTAAAAAATAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTTAAAAAAAAAACAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATTTCT  >  CP016816/484665‑484913

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: