Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816202,4850T.74.1% 224.8 / 284.1 218intergenic (+263/‑412)JCVSYN2_00840/JCVSYN2_00845hypothetical protein/lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (10/38);  new base . (61/76);  total (85/133)
Fisher's exact test for biased strand distribution p-value = 3.51e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.94e-01

Only 100 of 247 total aligned reads displayed.

AGTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCCTTTTAAAAAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAATATATATCTAATATA  >  CP016816/202359‑202604
                                                                                                                               |                                                                                                                        
 tttAGTGTAAATAATAGAGTTAATGTTAATTAAAATACCATAAACTTAATAATTATTATAATTTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCCtta                                                                                                                        <  1:230826/127‑2 (MQ=255)
 gTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATCCCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGATTATCTTCACAAATATAAAAAAAAAAATCACCtat                                                                                                                        >  2:2429263/1‑126 (MQ=255)
 gTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATACCAtta                                                                                                                        >  2:606503/1‑127 (MQ=255)
 gTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCATCACAAATAAATAAAAAACAAAACCCttt                                                                                                                        >  2:2258780/1‑128 (MQ=255)
 gTTAGTGAAAATAATAGAGTTAAGGTAAATTAAAATACCATAAACTTAATAATTCATATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCCtta                                                                                                                        <  1:221958/128‑2 (MQ=255)
   tatagtAAATAATATAGTTAAGGTTAAATAAAATAACATAAACTTAATAATTCATAAAATTTATATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCCttaaa                                                                                                                      <  1:1477506/124‑4 (MQ=255)
     gtgtAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCCttaaaaa                                                                                                                    >  1:2087484/1‑123 (MQ=255)
      tgtAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCACttaaaaat                                                                                                                   >  1:1953381/1‑122 (MQ=255)
          aataatAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAAACCCCttaaaaattaaa                                                                                                               >  2:1278073/1‑118 (MQ=255)
            taataGAGTTAAGGTTAAATAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTAATCTTCACAAATAAAAAAAAAACAATCCCC‑‑ttaaaaattaaaaa                                                                                                          >  2:114980/1‑128 (MQ=255)
             aataGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCCTT‑‑‑AAAAATaaaaaaa                                                                                                        >  1:2047461/1‑128 (MQ=255)
             aataGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCC‑‑ATtaaaaattaaaaaa                                                                                                         >  2:1926634/1‑128 (MQ=255)
               taGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAAAACCC‑‑TTAAAAATTaaaaaaaa                                                                                                       >  2:510956/1‑128 (MQ=255)
               taGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAACAATCACC‑‑TTAAAAATTaaaaaaaaa                                                                                                      >  1:261070/1‑128 (MQ=255)
                agagTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTTTTTATCTTCACAAATAAAAAAAAAAAAAATCACATT‑‑AAAAATaaaaaaaaa                                                                                                      >  2:886135/1‑128 (MQ=255)
                 gagTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCATAGTGATCTATATTTTTATTTGTTTATATTCACAAATAAAAAAAAAAAAAATCACATAaaaaaaaaaaaaaaaa                                                                                                       >  1:1260139/1‑128 (MQ=255)
                  agTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCC‑‑ATTAAAAAATaaaaaaaaaaa                                                                                                    >  2:1029659/1‑128 (MQ=255)
                   gTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTATTATTTGTTTATCTTAACAAATAAAAAAAAAAAAAATACCATAAAAAAATaaaaaaaaaaa                                                                                                    >  2:1884077/1‑128 (MQ=255)
                    ttAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCC‑‑TTAAAAATTaaaaaaaaaaaaa                                                                                                  >  2:2422649/1‑128 (MQ=255)
                     tAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCC‑‑ATTAAAAATTaaaaaaaaaaaaaa                                                                                                 >  2:2237188/1‑128 (MQ=255)
                     tAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTATATTTGTTTATCTTCACAAATAAAAAAAAAAAAATCCC‑‑‑ATAAAAAATtaaaaaaaaaaaaaa                                                                                                >  1:2062336/1‑128 (MQ=255)
                      aaGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGCTCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCACC‑‑‑TAAAAAATtaaaaaaaacaaaaaa                                                                                               >  2:1851660/1‑128 (MQ=255)
                      aaGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCC‑‑TTAAAAATTaaaaaaaaaaaaaaa                                                                                                >  1:2091427/1‑128 (MQ=255)
                      aaGGTTAATTAAAATACAATAAACTTAAAAATTCTTATAATGTAGATCATCGAGATCTATTTTATTATTAGTATATCTTAACAAATAAAAAAAAAACAAAAAC‑‑‑ATAAAAAATaaaaaaaaaaaaaaaa                                                                                               >  2:697578/1‑128 (MQ=255)
                       aGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATACCC‑‑TTAAAAATTaaaaaaaaaaaaaaaa                                                                                               >  2:2464906/1‑128 (MQ=255)
                        ggTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTTTTTATCTTAACAAATAAAAAAAAAACAATACCC‑‑‑TAAAAAATtaaaaaaaaaaaaaaaaa                                                                                             >  2:2194709/1‑128 (MQ=255)
                        ggTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCC‑‑TTAAAAATTaaaaaaaaaaaaaaaaa                                                                                              >  2:566676/1‑128 (MQ=255)
                        ggTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTATTTATCTTCACAAATAAAAAAAAAACAAACAACATaaaaaaaaaaaaaaaaaaaaaaaa                                                                                                >  2:1348800/1‑128 (MQ=255)
                        ggTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCATCGTGATCTATTTTTTTATTTTTTTATATTCACAAATAAAAAAAAAAAAAACAC‑‑ATTAAAAATTaaaaaaaaaaaaaaaaa                                                                                              >  2:1197551/1‑128 (MQ=255)
                         gTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCC‑‑TTAAAAATTaaaaaaaaaaaaaaaaaa                                                                                             >  2:230370/1‑128 (MQ=255)
                         gTTAATTAAAAATACAAAAAATAATAAATTATTATTATGTTTAACCTCTTGTTTTTTTTTTTTAATTGTTTATATTTACAAAATAAAAAAAAAAAATCCCC‑‑TTAAAAATTaaaaaaaaaaaaaaaaaa                                                                                             <  1:2034919/128‑1 (MQ=255)
                           taattaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTTGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATAACC‑‑TTAAAAATTaaaaaaaaaaaaaaaaaaaa                                                                                           >  1:2368260/1‑128 (MQ=255)
                           taattaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATACCCTT‑‑‑AAAAATaaaaaaaaaaaaaaaaaaaaa                                                                                          >  1:598537/1‑128 (MQ=255)
                           taattaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAAACACC‑‑‑TAAAAAATaaaaaaaaaaaaaaaaaaaaa                                                                                          >  2:2313971/1‑128 (MQ=255)
                            aattaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCATCACAAATAAAAAAAAAAAAA‑‑AAAAAATAAAAATTaaaaaaaaaaaaaaaaaaaaa                                                                                          >  2:1457109/1‑128 (MQ=255)
                                aaaaTACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTTTTTATCTTCACAAATAAAAAAAAAAAAA‑‑AACAATTAAAAATTaaaaaaaaaaaaaaaaaaaaaaaaa                                                                                      >  1:2406922/1‑124 (MQ=255)
                                 aaaTACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCACC‑‑TTAAAAATTaaaaaaaaaaaaaaaaaaaaaaaaaa                                                                                     >  1:1863758/1‑123 (MQ=255)
                                  aaTACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCAC‑‑ATTAAAAATTaaaaaaaaaaaaaaaaaaaaaaaaaac                                                                                    >  2:2105704/1‑122 (MQ=255)
                                  aaTACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATATGTTTATCTTCACAAATAAAAAAAAAACAATCCCC‑‑TTAAAAATTAaaaaaaaaaaaaaaaaaaaaaaaaaa                                                                                    >  1:1266080/1‑128 (MQ=255)
                                    taaaaTAAAATTAAAAATTATTATAATTTATATCATATTTATCTATTTTTTTATTTTTTTATATTAAAAAAAAAAAAAAAAAAAATCCCCTTAAAAATTAAAAAAAAAAAAAAAAAAAAAAACTaaaa                                                                                    <  1:2019300/124‑1 (MQ=255)
                                    aacaaTAAAATTAATAATTATTAAAATTTATATCCTAGTGATCTATTTTTTTATTTGTTTATATTAAAAAATAAAAAAAAAACAATCCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAaaaca                                                                                 <  1:1390544/124‑1 (MQ=255)
                                    aaacaaaaaaataataaTTATTATAAATTATATACACTTGTTATATTTTTTTATTTTTTTATATTAAAAAAAAAAAAAAAAAAAAAACCCATT‑‑AAAATTAAAAAAAAAAAAAAAAAAAAACTAAaaac                                                                                  <  1:1527878/117‑1 (MQ=255)
                                           aaCTTAAAAAATATTATAATTTAAATAATATTTATATATTTTTTTATTTTTTTATATTTAAAAAAAAAAAAAAAAAAAACCCC‑‑ATAAAAATAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAActct                                                                           <  1:2454398/128‑1 (MQ=255)
                                            aattAATAATTATTAAAATTTATATACTAGTTATCTATTTTTTTATTTTTTTTTTTTAAAAAATAAAAAAAAAACAATCCCC‑‑TTAAAAATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTa                                                                          <  1:1442305/126‑1 (MQ=255)
                                            aaatataaatTTTTATAATTTATAAAATCGTTTTTTATTTTTTTTTTTTTTTATATTTAAAAATAAAAAAAAAAAAAACCCCTTAAAAAATAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAActct                                                                           <  2:1917994/121‑1 (MQ=255)
                                             attAATTATTAATATAATGTTTATTATCGTGTAATATTTTTTTATTTTTTTATATTAAAAAAAAAAAAAAAAAAAAACCCCTTAAAAATTAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTa                                                                          <  1:67230/127‑1 (MQ=255)
                                             attAATAATTAATATAATTTATATCATAGTTATATATTTTTTTATTTTTTTATATTCAAAAAAAAAAAAAAAAAAAACCCC‑‑TTAAAAATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTAt                                                                         <  1:151982/127‑1 (MQ=255)
                                             aataaaaaattttATAAATTAGATACTCTTGATATATTTTTTTATTTTTTTATATTAAAAAAAAAAAAAAAAAAAAAACCCCTTAAAAAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTa                                                                          <  1:34526/117‑1 (MQ=255)
                                               taataaTTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCCGT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTTAaaaccaagagaatgtg                                                                      >  2:2076971/1‑119 (MQ=255)
                                                    attataaaaaTTTATATCCTCTTGATTTATTTTTTTATTTTTTAATCAAAAAAAAAAAAAAAAAAACAAATCCCAT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTaaa                                                                 <  2:437491/120‑1 (MQ=255)
                                                      tattATAATTTATATAATCGTTTTTTATTTTTTTTTTTTTTTTTTTTTAAAAATAAAAAAAAAAAAATCCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAAt                                                               <  1:1926634/126‑1 (MQ=255)
                                                        ttATAATTTTGATCCTCGTTTTGTATTTTTTTATTTTTTTATATTAACAAAAAAAAAAAAAACAATCCCC‑‑TTAAAAATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAAtt                                                              <  1:1967878/128‑1 (MQ=255)
                                                                   atactcgttttttATTTTTTTTTTTTTTTAATTTAAAAAAAAAAAAAAAAAAAAACCCC‑‑TTAAAAATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATAc                                                   <  2:238938/116‑1 (MQ=255)
                                                                    tcatgttGATCTATTTTTTTATATTTATACTTAAAAAATAAAAAAAAAAAAATACCC‑‑ATAAAAATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCATAAAATTCCCTATTATACca                                                 <  1:1620660/122‑1 (MQ=255)
                                                                    tacacgttataaaTTTTTTTATTTTTTATTTTTAACAAAAAAAAAAAAAAAAATACCC‑‑‑AAAAAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACca                                                 <  2:1224361/116‑1 (MQ=255)
                                                                      ctatttatatatttttttattttttTAAATTAAAAAATAAAAAAAAAAAAATACCCTT‑‑‑AAAAAT‑‑‑TAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACattt                                            <  1:1199052/119‑1 (MQ=255)
                                                                      aacGTTATCTATTTTTTTATTTGTTTAACTTCAAAAAAAAAAAAAAACAAACCC‑‑ATTAAAAATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACcaca                                               <  1:1852550/126‑1 (MQ=255)
                                                                       tatttttatATTTTTTTTTTTTTTTTTATTAAAAAAAAAAAAAAAAAAAATACCCTTAAAAATTAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACcac                                                <  1:461479/120‑1 (MQ=255)
                                                                       accttttcaatttttttatttttttATAATAAAAAAAAAAAAAAAAAAAAACACCATAAAAATTAAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACca                                                 <  1:1830039/119‑1 (MQ=255)
                                                                       acGTGATATAATTTTTTAATTTTTAAATTTCAAAAAAAAAAAAAAAACAATCCCCGTATAAAAAAAAAAAAAAAAAAAAAAAACAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTAATATACca                                                 <  1:2184581/127‑1 (MQ=255)
                                                                         tttatatatttttttattttttTATAATAAAAAAAAAAAAAAAAACAATCCAC‑‑TTAAAAATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACatt                                             <  1:1158771/122‑1 (MQ=255)
                                                                         gTGATTTAATTTTTTATTTTTTTATATTAAAAAATAAAAAAAAAACAAACCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACattt                                            <  2:1628364/128‑1 (MQ=255)
                                                                         gTGATATATTTTTTTATTTTTTTAAATTAAAAAAAAAAAAAAAAAAAAACCCC‑‑TTAAAAAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACatt                                             <  1:301041/128‑1 (MQ=255)
                                                                         ctgaataaTTTTTTTTTTTTTTTTTATTAAAAAAAAAAAAAAAAAAAAACCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACattt                                            <  1:1705331/121‑1 (MQ=255)
                                                                          ttttCTATTTTTTTATTTTTTTATCTTAACAAATAAAAAAAAAAAAATCCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACatttt                                           <  1:2241146/125‑1 (MQ=255)
                                                                           gATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCC‑‑TTAAAAATTAAAAAAAAAAAAAAAAAAAAATAAAAAAAAAATATATTTATTAAAATACAATATAATACAACatttta                                          >  1:1286178/1‑128 (MQ=255)
                                                                            tttatttttttttttttttTATTATTAATAATTAAAAAAAAAAAAATACC‑‑ATAAAAAATAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCAATTTCTTAAAATTCACTATTATACCACatttt                                           <  2:2350689/123‑1 (MQ=255)
                                                                            taattttttttttttttttTATATTAAAAAAAAAAAAAAAAAAAAAAACCAATTAAAATTAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACattt                                            <  1:389679/123‑1 (MQ=255)
                                                                                atttttttatttttttATATTAAAAAAAAAAAAAAAAAAAATACAC‑‑TTAAAAATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACattttatttt                                      <  1:178845/128‑1 (MQ=255)
                                                                                 tttttttatttttttaTATAAAAAAAAAAAAAAAAAAAAAACCAC‑‑TTAAAAATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTAttttt                                     <  2:2007418/128‑1 (MQ=255)
                                                                                  ttttttttttttttATTTTAAAATAAAAAAAAAAAAAAATACACTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTaaa                                  <  2:952092/128‑1 (MQ=255)
                                                                                  ttttttttttttttATATTAAAAAAAAAAAAAATAAAAATATCCTTAAATATTAAAAAAAAAAAAAAAAAAAAAATCTAAATACAAACTCTATTTCTTAAAATTCCCTATTATACCACattttatttt                                      <  1:2441297/128‑1 (MQ=255)
                                                                                   ttttttttttttTATATTAAAATAAAAAAAAAAAAAAATCAACTTAAAAATAAAAAAAAAAAAAAAAAAAAAAACTAAAAACAATCTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTa                                    <  1:2290523/128‑1 (MQ=255)
                                                                                   tttttATTTTTTTATATTAAAAAAAAAAAAAAAAAAAATACC‑‑ATTAAAAATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTaa                                   <  2:2371955/128‑1 (MQ=255)
                                                                                    ttttATTTGTTTAAATTAAAAAAAAAAAAAAAAACAATACCC‑‑TTAAAAATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTaaa                                  <  1:1377339/128‑1 (MQ=255)
                                                                                     tttATTTTTTTATATTTACAAATAAAAAAAAAACAATCCCCTT‑‑‑AAAAAT‑‑TAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATaa                              <  2:1978755/128‑1 (MQ=255)
                                                                                      ttATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAATAAAAACAAAAAATATTTATTAAAATTCCCTTTTTTACCACATTTTTTTTTTAAAATaa                              >  2:1309830/1‑128 (MQ=255)
                                                                                         ttttttttTATTAACAAATAAAAAAAAAACAAACCC‑‑ATTAAAAATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAg                             <  1:540906/128‑1 (MQ=255)
                                                                                         ttttttTATATTAAAAAAAAAAAAAAAAAAAATCCAC‑‑TTAAAAAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAg                             <  1:834938/128‑1 (MQ=255)
                                                                                         tttGTTTATCTTAACAAAAAAAAAAAAAAAAATCCCC‑‑TTAAAAAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAGAATTCCCTATTATACCACATCTTATTTTAAAAATaaa                             <  1:661993/128‑2 (MQ=255)
                                                                                          tattttATATTTAAAAAAAAAAAAAAAAAAAATCCCATTTAAAATTAAAAAAAAAAAAAAAAAAAAACTAAAAAAAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAg                             <  1:1176943/125‑1 (MQ=255)
                                                                                            gTTTATATTCTCAAAAAAAAAAAAAAAAATCACATTAAAAATTAAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGa                            <  1:1993863/128‑1 (MQ=255)
                                                                                             gtttatttaaaaaaaTAAAAAAAAAAAATACCGATAAAAAATAAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGat                           <  2:2179945/116‑1 (MQ=255)
                                                                                              ttttttttaaaaaaTAAAAAAAAAAAAATACCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTg                       <  2:2041907/117‑1 (MQ=255)
                                                                                              tttataataaaaaaaaaaaaaaaaaaaATCCCC‑‑TTAAAAAATAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATAtt                        <  1:1653555/117‑1 (MQ=255)
                                                                                               ttaattaaaaaaaTAAAAAAAAAAAATAACAATTAAAAATAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATAtt                        <  1:1633571/118‑1 (MQ=255)
                                                                                                 ttatttaaaaaaaaaaaaaaaaaaaaTCCCTTTT‑‑AAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGa                      <  2:287485/120‑1 (MQ=255)
                                                                                                   aaaaaaaaaaaaaaaaaaaaaaaaACCC‑‑TTAAAAAATAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACat                   <  1:2137336/122‑1 (MQ=255)
                                                                                                    ataacaAAAAAAAAAAAAAAAATAACC‑‑ATAAAAAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGatatatatta                   <  2:153456/125‑7 (MQ=255)
                                                                                                    ataaaaaaaaaaaaaaaaaaaATCAC‑‑ATTAAAAATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACat                   <  1:2336635/123‑1 (MQ=255)
                                                                                                     taaaaaaTTAAAAAAAAAAAAAACCCA‑‑TAAAAATTAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACat                   <  1:1751336/124‑1 (MQ=255)
                                                                                                     tCACAAATAAAAAAAAACAATCCCC‑‑TTAAAAATTAAAAAAAAAAAAAAAAAAAAATAAAAACAAAATCTATTAATTAAAATTACATATTAAACAAAATTTTATTTTTAAATAATGTTATTAAaataat                >  1:2076379/1‑128 (MQ=255)
                                                                                                      aaaaaaaaaaaaaaaaaaaATACCCTTAAAAATTAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACata                  <  1:10646/125‑1 (MQ=255)
                                                                                                      aaaaaaTAAAAAAAAAAAAATCACATTAAAAATTAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACata                  <  1:1299985/125‑1 (MQ=255)
                                                                                                         aaaaaaaaaaaaaaaaATCCCCTT‑‑‑AAAAAT‑‑TTAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAatatata           <  2:1897953/128‑1 (MQ=255)
                                                                                                           aTAAAAAAAAAACAATCCCCTTAAAAATTAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACACAATTAATAAAAATACAATATTAAAACAAAATTTATTTTTAAAATAATATATTAACAAAatata             >  1:2252284/1‑128 (MQ=255)
                                                                                                               aaaaaaaaaaATACCATTAAAAATAAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAATATATATc         <  1:1130684/128‑1 (MQ=255)
                                                                                                               aaaaaaaaCAATCCCATT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACAAAAAAAAACAGCTGGTTCTTAAAATTCCGTTTTATACCCAAATTTATTTTTAAATTAATATATTGAAAAaataaataagta       >  2:812658/1‑124 (MQ=255)
                                                                                                                 aaaaaaCAATCCCC‑‑TTAAAAATTAAAAAAAAAAAAAAAAAAAAACTAAAACCAACAGGTATTTGTAAAAATTCCAGTGTATACCGCATTTTTTTTTTTATATAAGATTTTGTATTAatatatattaaa      >  1:288791/1‑124 (MQ=255)
                                                                                                                   aaaaCAATCCCCTTAAAAATTAAAAAAAAAAAAAAAAAAAAAAATAAAAAAAAACTAAATTAAATAAAAATAAATAATATACAAAATTTTATTTTTAAAATAAAATATTTAAATAATAAATATATaaa     >  1:554015/1‑127 (MQ=255)
                                                                                                                               |                                                                                                                        
AGTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCCTTTTAAAAAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAATATATATCTAATATA  >  CP016816/202359‑202604

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: