Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816165,2500T.56.5% 73.1 / inf 885intergenic (+214/‑328)JCVSYN2_00665/JCVSYN2_00670tRNA N6‑adenosine(37)‑N6‑threonylcarbamoyltransferase complex dimerization subunit TsaB/tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base T (222/146);  new base . (349/130);  total (577/308)
Fisher's exact test for biased strand distribution p-value = 1.57e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1089 total aligned reads displayed.

ATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTAAAAGAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATGTTAATTAAAAC  >  CP016816/165123‑165377
                                                                                                                                  |                                                                                                                                
  aataTTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttt                                                                                                                                >  2:548035/1‑128 (MQ=255)
   atatTTAGTTACATTTATTTATCGCAATTTTTTGAATTAGTAATTAAATAAAAAATTTATATTTTTAGTTAATTTTTTTATATGAAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCattt                                                                                                                               <  1:2269845/128‑1 (MQ=255)
    tatTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttt                                                                                                                              >  1:2022576/1‑128 (MQ=255)
     atTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGATCTTGTAAAAAATAGAtttttt                                                                                                                             >  1:1089559/1‑128 (MQ=255)
     atTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttt                                                                                                                             >  2:2158405/1‑128 (MQ=255)
     atTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTAGAACTTGTAAAAAATATCtttttt                                                                                                                             >  1:2192933/1‑128 (MQ=255)
      tttAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttt                                                                                                                            >  2:2217879/1‑128 (MQ=255)
      tttAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttt                                                                                                                            >  2:1899616/1‑128 (MQ=255)
       ttAGTTTCATTAATTTATCGCACTTGTATGATTTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTTAACTTGTAAAAAATAGCtttttttt                                                                                                                           >  2:656895/1‑128 (MQ=255)
        tAGATTAATTAATTTATAGCACTTGTATGAATTATTAACAAAATAAAAATATTATCTTTTTAGATATTTTTTTTATATTCAAATTTTTAAAAAATTAAATTGAACTTGTAAAAAAAAGCttttttttt                                                                                                                          <  1:1800715/128‑1 (MQ=255)
          gTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttt                                                                                                                        >  1:91816/1‑128 (MQ=255)
          gTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGAAAATTATTAAAAAATTAATTTGAAATTGTAAAAAATATCttttttttttt                                                                                                                        >  1:1171886/1‑128 (MQ=255)
           tttCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttatttttt                                                                                                                       >  2:140958/1‑128 (MQ=255)
             tCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttt                                                                                                                     >  1:1740361/1‑128 (MQ=255)
               aTTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttttt                                                                                                                   >  2:2264744/1‑128 (MQ=255)
               aTTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATACAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATATCtatttttttttttatt                                                                                                                   >  2:1583366/1‑128 (MQ=255)
                ttAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGAttttttttttttatttt                                                                                                                  >  1:1662522/1‑128 (MQ=255)
                  aaTTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttttttta                                                                                                                >  1:567099/1‑127 (MQ=255)
                  aaTTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttttttat                                                                                                                >  1:1440208/1‑126 (MQ=255)
                    tttATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttttataatg                                                                                                              >  2:1626423/1‑122 (MQ=255)
                    tttATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttatttttttttaaa                                                                                                              >  2:515107/1‑125 (MQ=255)
                     ttATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttttttttaaaa                                                                                                             >  2:1054845/1‑124 (MQ=255)
                       aTCTCACTTTTTTTAATTACTTATAAAATAAAACAATTATATTTTTATATATTTTTTTTATATGCAAATTTTTAAAAAATTAATTTTAACATGTAAAAAATAGCTTTTTTTTTTTTTTTTtataatgg                                                                                                           <  1:41842/128‑5 (MQ=255)
                        tctctattGTTTTAATTATTTAGAAAATATAAATATTATTTTTTTATATTTTTTTTTTATATTCTAATTTTTTAAAAATTTATTTTAAATTTTAAAAAATATCTTTTTTTTTTTTTTTTTTATAATgg                                                                                                         <  1:2437885/122‑1 (MQ=255)
                        tctcaattttATTAATAATTAATAAAATAAAAATATTATCTTTTTATATATTTTTTTTAAATTCAAATTTTTAAAAAATTAATTTTAAATTTTAAAAAAAAGCTTTTTTTTTTTTTTTTTTATAATgg                                                                                                         <  1:321901/119‑1 (MQ=255)
                        tCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTATTTTTTTTTtataaatta                                                                                                          >  1:1204324/1‑123 (MQ=255)
                        tCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTCTATATGAAAATTTTTAAAAAATTAAATTGAACTTGTAAAAAATATAttttttttttttttttaataatata                                                                                                          >  1:2209345/1‑119 (MQ=255)
                         cGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTttattaattaaa                                                                                                         >  2:1471092/1‑122 (MQ=255)
                              ttGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTATTTTaatatgtatatat                                                                                                    >  2:934402/1‑119 (MQ=255)
                                  attaaTTTGTAAGAAAATAAAAATATTATATTTTTAGATATTTTTTGTATATTCAAATTTTTAAAAAATTAATTTTAACTTGTAAAAAAAA‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAATATAAAtt                                                                                              <  2:881259/125‑1 (MQ=255)
                                   tGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTAAATTTAAATATAAAttt                                                                                             >  1:1537938/1‑128 (MQ=255)
                                    ttaatAGTAAACAACAAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTTTACTTGTAAAAAAAACCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAAtt                                                                                              <  2:1972340/124‑1 (MQ=255)
                                     atttAGTCATAAACTTAAACTATTTATTTTTTAGATTTTTTTTGTTTATGCAATTTTTTAAAAATTTTAATTGAACTTTTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAg                                                                                          <  1:1299598/126‑1 (MQ=255)
                                        tAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATTGAAATTTAAATTTTAg                                                                                          >  2:1126143/1‑128 (MQ=255)
                                        tAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTTAATGTAAATATAAATTTTAgt                                                                                         >  2:2008065/1‑127 (MQ=255)
                                        tAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATGTAAATTAATAttttttg                                                                                         >  2:193430/1‑125 (MQ=255)
                                        atgtaattaaataaaaaaaTTATATTTTTTTTTATTTTTTTTTTATTGAAATTTTTAAAAAATTAATTTTAACTTTTAAAAAATACCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAgg                                                                                         <  2:2286834/117‑1 (MQ=255)
                                         atttaataaataaaaataTTATTTTTTTTTATATTTTTTTTATATTTAAATTTTTAAAAAAATAATTTTAAATTGTAAAAAAAACCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAgg                                                                                         <  1:1973878/118‑1 (MQ=255)
                                          ataataaATTAAAAAAATTATTTTTTTTTATTTTTTTTTTATATTTAAATTTTTAAAAAATTAATTTTTAATTTTTAAAAATATCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTa                                                                                       <  2:1173499/127‑1 (MQ=255)
                                              gAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATATTTAAAATAAAAATTTTattttttt                                                                                    >  1:239941/1‑121 (MQ=255)
                                              gAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTATTTTTTTTTTTTTATTTAATTTGAAATATTAAttttttggttttt                                                                                   >  2:1549796/1‑119 (MQ=255)
                                               aaactaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTTATTTAATTATAAATTTTATGTAtttttt                                                                                 >  1:71629/1‑126 (MQ=255)
                                                aactaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATTAAAATTTAAATTTTATGAATtttttt                                                                                >  1:1248621/1‑128 (MQ=255)
                                                aactaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTAAATGAAATATAAATTTTATGTtttttttttt                                                                              >  2:2382358/1‑126 (MQ=255)
                                                 actaaaactaTTATCTTTTTAGATATTATTTGTATATGCAAATATTTAAAAAATTAATTTGAACTTGTAATAAATATCTTTTTTTTTTTTTATTTTATAATTATAATATATTTTATATATAtttttat                                                                                >  1:351647/1‑125 (MQ=255)
                                                  ctaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGTAATTttgttg                                                                               >  1:1877751/1‑128 (MQ=255)
                                                   taaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATATTTGAAATATAAATTTTAGTTATTttgtttt                                                                              >  1:2310079/1‑126 (MQ=255)
                                                     aaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTTAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATTAAAATATAAATTTTATGTATTTTTTTTTCt                                                                            >  1:595577/1‑128 (MQ=255)
                                                     aaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATTGAAATATAAATTTTAGTTATttttttttat                                                                            >  2:368715/1‑124 (MQ=255)
                                                      aactaTTTACTTTTTAGTTATTTTTTGTTTTTGTAAATTTTTAAAAAATTAATTTGAAATTGTAAAAAAAAGC‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTaa                                                                          <  1:821860/128‑1 (MQ=255)
                                                      aaatattatCTTTTTAGATATTTTTTTTATATTCAAAATTTTAAAAAAATAATTTTAACTTTTAAAAAATAGCGTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTa                                                                           <  1:2358464/125‑1 (MQ=255)
                                                      aaagataatagttttAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTATTAT‑‑TGAAATATAAATTTTATTTTTTTTGTTTTATAAg                                                                         >  2:1272903/12‑128 (MQ=255)
                                                           ttatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTAATATTTAAATATTAATTTAAGTTATttttttttatatttata                                                                     >  2:262718/1‑117 (MQ=255)
                                                            tatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTATGTATTTTTTTTTATAATtatata                                                                   >  1:1147473/1‑128 (MQ=255)
                                                                  tttAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTAGTAAAAAATAGCTTTTTTTTTTTTTTTTTATTTATAAAATATAAATTTAAGGTATATTTTTTTATAATATACaaaaaaa                                                            >  2:778794/1‑128 (MQ=255)
                                                                   ttAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATATTGTAAATATAAATTTTTTGTTTTTTGTTTTCTAATTATAaaaaaaaa                                                            >  2:1879111/1‑128 (MQ=255)
                                                                      ttttttttttttATATTTAAAATTTTTAAAAAATAATTTTTAATTTTAAAAAATA‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAg                                                         <  1:2155485/124‑1 (MQ=255)
                                                                       tttttttttttttATGCAAATTTTTAAAAAAATTATTTTTAATTTTAAAAAATTTTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAg                                                         <  1:193208/125‑1 (MQ=255)
                                                                       ttatTTTTTTTTTATTCAAAATTTTAAAAATATTATTTTAAATTTTAAAAAATA‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGa                                                        <  1:139732/127‑1 (MQ=255)
                                                                       attttttttttATTGAAATTTTTTAAAAATTTATTTTTAAATTTTAAAAAAATCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGaa                                                       <  2:183567/125‑1 (MQ=255)
                                                                        tatTTTTTGTATATTCAAATTTTAAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTATTTTATTTAAATAAAAATTAAAATTTAATGAATTTTATAGTCAAAGTAACTaaaaaaaaata                                                        >  2:2113525/1‑124 (MQ=255)
                                                                         atTTTTTTTATTTGTAATTTTTTAAAAAATTAATTTTTAATTTTAAAAAATA‑‑‑TATTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAg                                                     <  1:1016414/128‑1 (MQ=255)
                                                                          ttttttGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATTTAAATAAAAATTTTATTTTTTTTTTTTTATAATTTTAaaaaaaaaaaaaaaaa                                                    >  1:169587/1‑126 (MQ=255)
                                                                              ttttttttattaaaTTTTATAAAAAAATAATTTTAAATTTAAAAAAAATCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAgg                                                    <  2:690529/117‑1 (MQ=255)
                                                                               tttatatttaaaaTTTTTAAAAATTTATTTTTAATTTTTAAAAAAATCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGaaa                                                 <  2:314653/118‑1 (MQ=255)
                                                                                gTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATGCAAATATAAATTTTAGTTATTTTGTTGTATAATTATATAAAAAAATAGAAAGGaaaa                                                >  1:1899736/1‑128 (MQ=255)
                                                                                  atatGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATGTAAATAAAAATTTTAGTTATTTTTTTTTATATGTATAAAAAAAAATAGAAAGGaaaaaa                                              >  1:977565/1‑128 (MQ=255)
                                                                                   tatGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTAAAATTTAAATATAAATTTTATGTTTTTTTTTTTATAATTATATAAAAAAATAAAAAGAAAAAAat                                             >  2:597824/1‑128 (MQ=255)
                                                                                    atGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTAGGTTTTTTGTTTTATAAGTATATAAAAAAATATAAATGAAAAAattt                                           >  1:884586/1‑126 (MQ=255)
                                                                                     tGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATGTAATTATAAATTTTAGTTTTTTTGTTTAATAAGTATATAAAAAAATAGAAAGTAAATAatat                                           >  1:2234283/1‑128 (MQ=255)
                                                                                     attcataTTTTTTAAAAATTATTTATAAATTTTAAAAAATTTCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAata                                            <  1:2465385/122‑1 (MQ=255)
                                                                                        aaaaTTTTTAAAAATTAATTTTAAAATGTAAAAAATA‑‑‑TCATTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTa                                      <  1:63703/127‑1 (MQ=255)
                                                                                         ataTTTTTAAAAAATAATTTTTAATTGTTAAAAATATTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGtt                                       <  2:1361108/126‑1 (MQ=255)
                                                                                           aTTTTAAAAAAATAAATTTATCATTTAAAAAAATTTTATTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTaa                                     <  2:2422501/128‑1 (MQ=255)
                                                                                              tttATAAAAATTAATTTTAAATTTTATAACATAGCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTaaa                                    <  1:297518/128‑1 (MQ=255)
                                                                                                  taaaaaaTTAATTTTAAATTTTAAAAAATATTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGt                                 <  2:810229/127‑1 (MQ=255)
                                                                                                   aaaaaaTTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTTAATGTAATTAAAAATTTAATGTTTTTTTTTTTATAAGTTTATAAAAAAAAATAAATGAAAAATTTTTTTTTAAAGTa                                >  2:2456307/1‑128 (MQ=255)
                                                                                                    aaaaattaattTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATATTGGAAATATAAATTTTAGGTTTTTTGTTTAATATGTATGAAAAAAAATAAAAAGGAAAAAATATTGTTAAAATTaa                               >  2:176320/4‑128 (MQ=255)
                                                                                                     aaaattaattTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATTGAAATTTAATTTTTAGGTTTTTTTTTGTATAATTTTATAAAAAAATTAAAAGTAAAAAAATTTGTTAAAAGTaa                               >  1:1571796/3‑128 (MQ=255)
                                                                                                      aaattaattTTAAATTTTTAAAAATTTTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAATa                             <  1:209137/127‑1 (MQ=255)
                                                                                                       aattaattTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTT‑‑TAATGTAATTATAAATTTTAGTTATTTTTTTTTATAATAATATAAAAAAAAAAAAAGAAAAAAATTTTTTTaaatttataaat                           >  1:4930/1‑119 (MQ=255)
                                                                                                       aattaattTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTAAAATTGTAATATAAATTTTTGGTATTTTTTTTGCAAAATATAAAAAAAAAAACAAATGAAAAAATATATATAAAAATAAAAca                           >  2:2320031/1‑127 (MQ=255)
                                                                                                        attaattTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATATTGAAAATATAATTTTTTGTTTTTTTTTTTTATAATTTTATAAAAAAATTAAAAGTAAAAAATTTTTTTaaatttataata                           >  1:775558/1‑119 (MQ=255)
                                                                                                        attaattTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTAAAATGGAAATATAATTTTTAGGTTTTTTTTTGTCAAATTTAAAAAAAAAATAGAAATGAAAAATATTTTTTAAAATTAATaaa                           >  2:1302607/1‑126 (MQ=255)
                                                                                                         ttaattTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATTGTAATATAAATTTTTGGTTTTTTGTGTGATATGTTACTAAAAAAATGTAATGGAAAAAAATTTTTTTAAAGTATTACTa                          >  1:523963/1‑128 (MQ=255)
                                                                                                         ttaattTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGGAAATAAAAATTTTAGGTTTTTTTTTTTCTAATTATATAAAAAAATAAAAAGTAAAAAATATTTTTATAATtaataataa                         >  2:462953/1‑128 (MQ=255)
                                                                                                         ttaattTGAACTTGTAAAAAATAGATTTTTTTTTTTTTTTTTTAAAAAGTAAATATAAATTATAGATATTTTGTTTTCTAATTATATATAAAAATATAAAATAAAAAATATTTTTAAAAGaaaaaata                          >  2:849169/1‑123 (MQ=255)
                                                                                                          taattTTATATTTTAAAAAATA‑‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAATACTaaa                        <  2:169576/128‑1 (MQ=255)
                                                                                                            attTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTTAATGGAAATATAAATTTTAGTTTTTTTTTTTTATAAGTAAATAAAAAATATAAAAATAAAAAATTTTTTTAAAATTAATAaaaaaa                       >  2:2058099/1‑128 (MQ=255)
                                                                                                              ttGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATGGAAATAAAAATTTTTGGTATTTTGTTGTTTATGTATAAAAAAAAATAAAAAGGAAAAAAATTTGTTAAAAGTAAAACTTAAatt                     >  1:132842/1‑128 (MQ=255)
                                                                                                              ttGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTTTGTATTTTTTTGTAAAATTATATAAAAAAATATAAATTAAAAAATATTTTTAAAATTAATAATAAAatta                    >  1:847854/1‑128 (MQ=255)
                                                                                                                  aCTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTATGTATTTTTTTGTATAATTATATAAAAAAATATAAAGTAAAAAATTTTTTTAAAATTAATAATAAAATTATTTa                >  1:1481805/1‑128 (MQ=255)
                                                                                                                      ttttataatatttttttttttttttttttttATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAAt              <  2:420755/116‑1 (MQ=255)
                                                                                                                      gTAAAAAATAGCTTTTTTTTTTTTTTTTTTAAAATGTAAATAAAAATTTTATTTATTTTTTTTTATAATTATATAAAAAATTATAAATGAAAAAATATTTTTAAAATTTATAATAAAattattatatt             >  1:737907/1‑123 (MQ=255)
                                                                                                                       tttaaaaaatttttttttttttttttttttATAATTGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATg             <  1:451947/117‑1 (MQ=255)
                                                                                                                       tAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTATGTATTTTTTTTTATAATTATAAAAAAAAAAAAAAAGTAAAAAAAATTTTTAAAAGTAAAAAAAAAATTATTTatttt            >  2:1238697/1‑124 (MQ=255)
                                                                                                                       tAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATGAAAATATAAATTTTTGTTATTTTTTTGTATAAGTATATAAAAAAATATAAAGTAAAAAATATTTTTAAAATTATAAAAAAAATTATATAAttt            >  1:1608029/1‑126 (MQ=255)
                                                                                                                          aaaaTAGCTTTTTTTTTTTTTTTTTTTATATTGTAAATATAATTTTTTTGTTTTTTGTTGTATTTGTATATAAAAAATTATAAAGAAAAAAATTTTTTTAAATGTAATTCTAAAATTTttttatttta          >  1:669193/1‑128 (MQ=255)
                                                                                                                           aaaTAGCTTTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTAGGTTTTTTGTTGTCTAATTATATAAAAAAATTGAAAGGAAAAAATATTTTTAAAATTATTACAAAAATTAtttaattttaat        >  2:500503/1‑128 (MQ=255)
                                                                                                                           aaaTAGCTTTTTTTTTTTTTTTTTTATAATGGAAACATAAATTTTATGTATTTTGTTTTCTAAGTTTATAAAAAAATAGAATGGAAAAAATATTGTTAATATTAATACTAAAATTATTTAATGtttat        >  1:2040761/1‑128 (MQ=255)
                                                                                                                                  |                                                                                                                                
ATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTAAAAGAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATGTTAATTAAAAC  >  CP016816/165123‑165377

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: