BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP016816	20,023	2 bp→TA	intergenic (‑744/‑392)	JCVSYN2_00065 ← / → JCVSYN2_00070	hypothetical protein/tetracycline resistance ribosomal protection protein Tet(M)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP016816	20,023	0	A	T	99.9%	4167.4 / NA	1402	intergenic (‑744/‑393)	JCVSYN2_00065/JCVSYN2_00070	hypothetical protein/tetracycline resistance ribosomal protection protein Tet(M)
Reads supporting (aligned to +/- strand): ref base A (0/1); new base T (799/602); total (799/603)
Fisher's exact test for biased strand distribution p-value = 4.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	CP016816	20,024	0	T	A	99.7%	4222.2 / NA	1402	intergenic (‑745/‑392)	JCVSYN2_00065/JCVSYN2_00070	hypothetical protein/tetracycline resistance ribosomal protection protein Tet(M)
Reads supporting (aligned to +/- strand): ref base T (1/3); new base A (798/600); total (799/603)
Fisher's exact test for biased strand distribution p-value = 3.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1418 total aligned reads displayed.

GAGCAGGAAGAGCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATAATTTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGAAAAAACAAAAAAAG  >  CP016816/19899‑20145

||

  gCAGGAAGAGCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAACAACTATTTTTTCTTTAAATTCGTATTTTACTTTCTATTTTTAATTTATATATTta                                                                                                                       >  2:3014578/1‑128 (MQ=255)

    aGGAAGAGCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTtata                                                                                                                     >  2:1001176/1‑128 (MQ=255)

     ggAAGAGCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTAAATTTCTTTTTTTACTTTCTATTTTTAatttatatatttatat                                                                                                                    >  2:433221/1‑128 (MQ=255)

        agagCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTaa                                                                                                                 <  1:2657266/128‑1 (MQ=255)

          agCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTaaaa                                                                                                               >  2:3172254/1‑128 (MQ=255)

            cAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGTAAAACAAAAACTATATTTACTTTAATTACTTTTTTTACTTTCTATTTTTAATTATTATATTTATATTaaaaaa                                                                                                             >  1:1052444/1‑128 (MQ=255)

             aaGATAAAATGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTGTACTTTCTATTTTTAATTTATATATTTATATTAaataat                                                                                                            >  2:1704880/1‑128 (MQ=255)

               gATAAAAGGTAGTATTTTTTGGCGATTCCCCTAGATTCTTTTTCATATTAGGAAAAAAAAAACTATTTTTTCTTTAAATTATTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAAttt                                                                                                          <  1:354343/128‑1 (MQ=255)

               gATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAAttt                                                                                                          >  2:549334/1‑128 (MQ=255)

                 tAAACGGCAGCAATTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTaa                                                                                                        <  2:1487417/128‑1 (MQ=255)

                 tAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTTATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTaa                                                                                                        >  1:2462298/1‑128 (MQ=255)

                  aaaaGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTaaa                                                                                                       >  1:2521806/1‑128 (MQ=255)

                   aaaGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAaat                                                                                                      >  1:1126685/1‑128 (MQ=255)

                    aaGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAaatt                                                                                                     <  2:1941543/128‑1 (MQ=255)

                        tagtaTTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAaattataa                                                                                                 >  2:773820/1‑128 (MQ=255)

                          gtaTTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTaa                                                                                                        >  1:79886/1‑119 (MQ=255)

                          gtaTTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAaagtataatt                                                                                               >  1:672540/1‑128 (MQ=255)

                          gtaTTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTATTTAATTTGGTTTTTTACATTCAAATTTTAAATTATATATTTAAATTAAAAAATTTAaattataaat                                                                                               >  1:95214/1‑126 (MQ=255)

                           tagttgtggGCGATCGCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAaattataatta                                                                                              <  1:2138654/125‑1 (MQ=255)

                             tttGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAattatt                                                                                            <  1:1001176/128‑1 (MQ=255)

                             tttGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTATTTCTTAAATTTCTTAGTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAAATTAAATTTTAattatt                                                                                            >  1:141143/1‑128 (MQ=255)

                                  tgGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTtat                                                                                       <  1:2850860/128‑1 (MQ=255)

                                     cGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGc                                                                                    >  2:207605/1‑128 (MQ=255)

                                     cGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATATATATTAAAAAATTTAAATTATAATTATTTTTATAGc                                                                                    >  1:2812189/1‑128 (MQ=255)

                                      gATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCa                                                                                   >  2:64823/1‑128 (MQ=255)

                                         cccccTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGt                                                                                >  2:2007258/1‑128 (MQ=255)

                                           cccTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGa                                                                              >  2:1071215/1‑128 (MQ=255)

                                            ccTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACTTGAt                                                                             >  1:3050385/1‑128 (MQ=255)

                                            ccTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGAt                                                                             <  1:318464/128‑1 (MQ=255)

                                            ccTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCATTAATTTCTTTTTTTACTTTATATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGAt                                                                             >  1:2603644/1‑128 (MQ=255)

                                               agagTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTtat                                                                                                                      <  1:2268783/84‑1 (MQ=255)

                                               agagTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAAt                                                                          <  2:2670358/128‑1 (MQ=255)

                                                  gTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTaa                                                                       >  2:3235414/1‑128 (MQ=255)

                                                    cTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTaaaa                                                                     >  1:851755/1‑128 (MQ=255)

                                                    cTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTaaaa                                                                     >  1:2229752/1‑128 (MQ=255)

                                                    cTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTaaaa                                                                     >  1:1333100/1‑128 (MQ=255)

                                                        tACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTa                                                                 >  2:2612355/1‑128 (MQ=255)

                                                        tACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTAAATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTa                                                                 >  1:1674633/1‑128 (MQ=255)

                                                        tACATCATCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTa                                                                 >  1:3144821/1‑128 (MQ=255)

                                                         aCATCTTCTGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAg                                                                <  2:2365914/128‑1 (MQ=255)

                                                         aCATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATATCACTTGATAATTAAAAGTTAg                                                                >  1:2319638/1‑128 (MQ=255)

                                                         aCATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAg                                                                >  1:404380/1‑128 (MQ=255)

                                                         aCATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATATAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAg                                                                >  1:1284819/1‑128 (MQ=255)

                                                          cATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACTTGATAATTAAAAGTTAGt                                                               >  1:608847/1‑128 (MQ=255)

                                                          cATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACTTGATAATTAAAAGTTAGt                                                               >  2:3083998/1‑128 (MQ=255)

                                                          cATATTCGGAAAACAAAAACTATTTTTTCTTTAATTACTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTATAATTATAATTATTTATATAGCACGTAATAATTAAAAGATaat                                                               >  1:2094380/1‑126 (MQ=255)

                                                              ttaggAAAACAAAAACTATTTTTTCTTTAATTTATTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAAc                                                           <  2:889769/125‑1 (MQ=255)

                                                               tCGGAAAACAATAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACa                                                          <  2:2435252/128‑1 (MQ=255)

                                                                cGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATATCACGTGATAATTAAAAGTTAGTGAACaa                                                         >  1:1725912/1‑128 (MQ=255)

                                                                   aaaaCAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAATTTAGTGAACAAGaa                                                      >  2:1421052/1‑128 (MQ=255)

                                                                    aaaCAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAAtt                                                                         >  1:2375212/1‑108 (MQ=255)

                                                                    aaaCAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGaaa                                                     <  2:162734/128‑1 (MQ=255)

                                                                         aaaaCTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGt                                                <  1:738782/128‑1 (MQ=255)

                                                                         aaaaCTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGt                                                >  1:1872459/1‑128 (MQ=255)

                                                                          aaaCTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTg                                               >  2:934158/1‑128 (MQ=255)

                                                                            aCTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGATAACAGTGaa                                             <  1:1973552/128‑1 (MQ=255)

                                                                            aCTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGaa                                             <  1:3097816/128‑1 (MQ=255)

                                                                             cTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAg                                            <  1:522231/128‑1 (MQ=255)

                                                                              tATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGc                                           >  2:716885/1‑128 (MQ=255)

                                                                               aTTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCa                                          >  2:2314086/1‑128 (MQ=255)

                                                                               aTTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCa                                          >  2:1540975/1‑128 (MQ=255)

                                                                                 tttttCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCAcc                                        <  1:1480554/128‑1 (MQ=255)

                                                                                  ttttCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCa                                       <  1:1922293/128‑1 (MQ=255)

                                                                                   tttCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAg                                      >  2:60310/1‑128 (MQ=255)

                                                                                    ttCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGt                                     >  1:916236/1‑128 (MQ=255)

                                                                                     tCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTTAAGCACCAGtt                                    >  2:1805912/1‑128 (MQ=255)

                                                                                     tCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGtt                                    <  1:2952230/128‑1 (MQ=255)

                                                                                     tCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGtt                                    >  1:3003775/1‑128 (MQ=255)

                                                                                      cTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGttt                                   >  1:2039072/1‑128 (MQ=255)

                                                                                      cTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGttt                                   >  2:1165438/1‑128 (MQ=255)

                                                                                      cTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCAAGTGATAATTAAAAGTTATTGAACAATAAAACAGTGAAGCACAAGttt                                   >  2:2766041/1‑128 (MQ=255)

                                                                                        ttAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCt                                 <  2:212728/128‑1 (MQ=255)

                                                                                        ttAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCt                                 <  2:2181648/128‑1 (MQ=255)

                                                                                        ttAATATCTTTTTTTACTATCTATTTTTAATTTATATATTTATATAAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCt                                 <  2:1346232/128‑1 (MQ=255)

                                                                                        gaatttgttgtatttACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCt                                 <  1:1565787/116‑1 (MQ=255)

                                                                                         tAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTg                                <  2:2953062/128‑1 (MQ=255)

                                                                                           aTTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGaa                              <  2:2830313/128‑1 (MQ=255)

                                                                                             ttCTTTTTTTACTTTCTTTTATTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAAcc                            <  2:1748433/128‑1 (MQ=255)

                                                                                             ttCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAAcc                            <  1:1618686/128‑1 (MQ=255)

                                                                                              tCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCa                           <  2:3277213/128‑1 (MQ=255)

                                                                                                tttttttACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCTCCAGTTTCTGAACCaaa                         >  2:818802/1‑128 (MQ=255)

                                                                                                tttttttACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCaaa                         >  1:2727608/1‑128 (MQ=255)

                                                                                                tttttttACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATATTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCaaa                         <  1:1807738/128‑1 (MQ=255)

                                                                                                 ttttttACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCaaaa                        <  1:1211329/128‑1 (MQ=255)

                                                                                                 ttttttACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCaaaa                        <  2:2506083/128‑1 (MQ=255)

                                                                                                     ttACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAaagaag                    <  2:2557445/128‑1 (MQ=255)

                                                                                                     ttACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAaagaag                    >  2:1863947/1‑128 (MQ=255)

                                                                                                      tACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAagaaga                   >  1:47244/1‑128 (MQ=255)

                                                                                                      tACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAagaaga                   >  2:1228667/1‑128 (MQ=255)

                                                                                                       aCTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGAc                  >  2:2888102/1‑128 (MQ=255)

                                                                                                          ttCAATTTATATTTTATATATTTATATTAAAAAATTAAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGaa               <  1:560680/128‑1 (MQ=255)

                                                                                                           tCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGaaa              >  2:366051/1‑128 (MQ=255)

                                                                                                           tCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGATTCTGAACCAAAAGAAGACGaaa              >  2:1276089/1‑128 (MQ=255)

                                                                                                            cTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGaaaa             >  2:2713330/1‑128 (MQ=255)

                                                                                                              aTTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGaaaaaa           >  1:3248365/1‑128 (MQ=255)

                                                                                                               tttttAATTTATATATTTATATTAAAAAATTTAAATTATAAATATTTTTATAGCACGTGATAATTAAAAGATAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGAAAAAAc          <  2:2245891/128‑1 (MQ=255)

                                                                                                                  ttAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGAAAAAACaaa       >  1:871197/1‑128 (MQ=255)

                                                                                                                    aaTTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGAAAAAACaaaaa     <  1:2184040/128‑1 (MQ=255)

                                                                                                                    aaTTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGAAAAAACaaaaa     >  2:510529/1‑128 (MQ=255)

                                                                                                                       ttATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGAAAAAACAAAAAAAg  >  1:2894640/1‑128 (MQ=255)

||

GAGCAGGAAGAGCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATAATTTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGAAAAAACAAAAAAAG  >  CP016816/19899‑20145

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`