Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 165,218 A→T intergenic (+182/‑360) JCVSYN2_00665 → / → JCVSYN2_00670 tRNA N6‑adenosine(37)‑N6‑threonylcarbamoyltransferase complex dimerization subunit TsaB/tRNA‑Ser

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816165,2180AT97.0% 3642.7 / 5.3 1256intergenic (+182/‑360)JCVSYN2_00665/JCVSYN2_00670tRNA N6‑adenosine(37)‑N6‑threonylcarbamoyltransferase complex dimerization subunit TsaB/tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base A (10/27);  new base T (803/416);  total (813/443)
Fisher's exact test for biased strand distribution p-value = 4.06e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1291 total aligned reads displayed.

TATTTACTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTAAAAGAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGT  >  CP016816/165091‑165346
                                                                                                                                  |                                                                                                                                 
  tttACTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTtaa                                                                                                                                 <  2:2446961/128‑1 (MQ=255)
  tttACTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTtaa                                                                                                                                 <  2:1895793/128‑1 (MQ=255)
  tttACTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTtaa                                                                                                                                 <  2:1500012/128‑1 (MQ=255)
  tttACTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTtaa                                                                                                                                 <  2:1550975/128‑1 (MQ=255)
  tttACTTTAATAGAATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTtaa                                                                                                                                 <  1:1273058/128‑1 (MQ=255)
     aCTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTtaaaaa                                                                                                                              >  2:2776162/1‑126 (MQ=255)
      cTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTaaaaaa                                                                                                                             >  1:526372/1‑128 (MQ=255)
       tttAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaat                                                                                                                            >  2:3326275/1‑128 (MQ=255)
       tttAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTAATATATTTTTAGATAATTTTTGTATATGCAAATTTTAAAAAaat                                                                                                                            >  2:72995/1‑128 (MQ=255)
           aTTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaattaat                                                                                                                        >  2:676608/1‑128 (MQ=255)
           aTTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaattaat                                                                                                                        >  1:109099/1‑128 (MQ=255)
            ttGTATTACTTTACTAGTTGATAATATTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAAttt                                                                                                                      <  2:2193952/128‑1 (MQ=255)
             tGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAAttt                                                                                                                      >  2:2049925/1‑128 (MQ=255)
             tGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAAttt                                                                                                                      <  2:3080232/128‑1 (MQ=255)
             tGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAAttt                                                                                                                      <  1:405267/128‑1 (MQ=255)
             tGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAAttt                                                                                                                      <  2:2517369/128‑1 (MQ=255)
                aTTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGaa                                                                                                                   >  2:858210/1‑128 (MQ=255)
                aTTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGaa                                                                                                                   <  2:812524/128‑1 (MQ=255)
                aTTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAAATATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGaa                                                                                                                   >  1:1412637/1‑128 (MQ=255)
                     tttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTATAACTATTATCTTTTTAGATATTTATTCTATATGCAAATTTTTAAAAAATTAATTTGAACTTGt                                                                                                              >  1:1755352/1‑128 (MQ=255)
                     tttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGt                                                                                                              >  2:1767457/1‑128 (MQ=255)
                     tttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGt                                                                                                              >  1:1494734/1‑128 (MQ=255)
                     tttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATAATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTTAACTAGt                                                                                                              >  2:1417428/1‑128 (MQ=255)
                     tttactAGTTGATAATATTTAGTTTCAGTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACtttt                                                                                                              >  1:2450668/1‑126 (MQ=255)
                      ttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTa                                                                                                             >  1:337670/1‑128 (MQ=255)
                        actAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaaa                                                                                                           >  1:2015394/1‑128 (MQ=255)
                        actAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaaa                                                                                                           >  2:1282909/1‑128 (MQ=255)
                        actAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaaa                                                                                                           <  2:2092926/128‑1 (MQ=255)
                        actAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaaa                                                                                                           >  1:3195318/1‑128 (MQ=255)
                        actAGTTGATAATATTTAGTTTCATTAATTTATCGAACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaaa                                                                                                           <  1:3103883/128‑1 (MQ=255)
                         ctAGTTGATAATATTAAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaaaa                                                                                                          <  1:1008904/128‑1 (MQ=255)
                          tAGTTGATAATATTTAGTATCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaaaaa                                                                                                         <  1:3153987/128‑1 (MQ=255)
                            gTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTTTAAAAAAt                                                                                                       >  2:1348907/1‑128 (MQ=255)
                                 taataTTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAAATATTAAAAAATTAAATTGAACTTGTAAAAAATAGCtt                                                                                                  >  2:525540/1‑128 (MQ=255)
                                 taataTTTAGTTCCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaaaa                                                                                                          >  2:320598/1‑120 (MQ=255)
                                  aataTTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttg                                                                                                 >  1:775029/1‑127 (MQ=255)
                                   atatTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttt                                                                                                >  1:276374/1‑128 (MQ=255)
                                    tatTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTTAACTTGTAAAAAATAGCttttt                                                                                               >  2:261171/1‑128 (MQ=255)
                                      tttAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttt                                                                                             >  2:2365117/1‑128 (MQ=255)
                                        tAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttt                                                                                           >  2:1049083/1‑128 (MQ=255)
                                        tAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttt                                                                                           >  1:1141201/1‑128 (MQ=255)
                                        tAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttt                                                                                           >  2:1995638/1‑128 (MQ=255)
                                             tCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttt                                                                                      >  1:2356729/1‑128 (MQ=255)
                                               aTTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttttt                                                                                    >  2:2711465/1‑128 (MQ=255)
                                                ttAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttttttt                                                                                   >  2:1116508/1‑128 (MQ=255)
                                                ttAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttttttt                                                                                   >  2:2561548/1‑128 (MQ=255)
                                                ttAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTATGTATATGCAAATTTTTAAAAAATTAATATGAACTTGTAAAAAATAGCttttttttttttttttt                                                                                   >  2:2925975/1‑128 (MQ=255)
                                                   aTTAATCGCACTTGTATTAAATAGTAAGAAACAAAAACTATTAACTTTTTAGATATTTTTTGTATATGCAAAATTTTAAAAAATTAAATTGAACATGTAAAAAATAGCttttttttttttttttttat                                                                               <  2:1844676/128‑3 (MQ=255)
                                                     ttatatcaaatGTATGAATTATAAATAAACTAAAACTATTAAGTTTTTAGATATTTTTTGTATATGAAAAATTTTAAAAAAATAAATTTTAATTGTAAAAAAAAGCATTTTTTTTTTTTTTTtataat                                                                             <  2:2142949/118‑3 (MQ=255)
                                                      tATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGATCTTGTAAAAAATAGAttttttttttttttttttaaaat                                                                            >  2:2869449/1‑123 (MQ=255)
                                                      tATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATTGCTTTTTTTTTTTTTTTTtataatt                                                                            >  1:1695567/1‑125 (MQ=255)
                                                       ttagcaaatGTATGAATTTTTAACAAACTAAAAATTTTATCTTTTTTGATATTTTTTGTAAATTCAAATTTTTAAAAAATTAATTTGTACTTGTAAAAAATAGCCTTTTTTTTTTTTTTTTTATAATg                                                                          <  2:1656283/120‑1 (MQ=255)
                                                              ttGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTTAATTTAAatat                                                                   >  2:1610692/1‑128 (MQ=255)
                                                                gTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATA‑‑TCTTTTTTTTTTTTTTTTTATAATTTAAATATaaa                                                                >  2:462558/1‑128 (MQ=255)
                                                                 tatttaTTTGTAATAAAATAAAACATTTATATTTTTTTTTTTTTTTTTTATATGCAAATTTTTAAAAAATTTATTTGAAATTTTAAAAAAAAGCTTTTTTTTTTTTTTTTTTATAATGGAAATATaaa                                                                <  1:2562557/123‑1 (MQ=255)
                                                                  aagatattgtcaGAAAATTAAAATATTATATGTTTTGATATTTTTTGTATAAGCATATATTTAAAAAATTAATTTGTACTTTGAAAAAATA‑‑TCGTTTTTTTTTTTTTTTTATAATGGAAATATAAAtt                                                              <  1:3017860/117‑1 (MQ=255)
                                                                     aaTTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTTTAATTTAAATATAAtttt                                                             >  1:1936739/1‑128 (MQ=255)
                                                                      aTTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATGTAAATATAAAtttaa                                                           >  2:298902/1‑126 (MQ=255)
                                                                        tatttaaaaaataaaaattTTATTCTTTTTTTTTATTTTTTTTTATTTAAATTTTTTAAAAAATTAATTTTAAATTTAAAAAAAATCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAgg                                                         <  2:1696286/121‑1 (MQ=255)
                                                                        tattaataaAATAAAAATTTTTTTTTTTTTTATATTTTTTTTATTTTCAAATTTTTTAAAAATTAATTTTAAATTTTTAAAAATA‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGt                                                        <  2:944217/125‑1 (MQ=255)
                                                                        tAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGATTTTTTTTTTTTTTTTTTATAATGTAAATATAAAttttttt                                                         >  2:772099/1‑125 (MQ=255)
                                                                        atttaataaAATAAAAATATTATTTTTTTATATATTTTTTGTATATTAAAATTTTTAAAAAATTAATTTTAAATTTTAAAAAAAAGCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAgg                                                         <  2:3040179/125‑1 (MQ=255)
                                                                         aGTAAGAAACTTAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATTAAAAAATAAATTTTatttt                                                       >  1:2802377/1‑124 (MQ=255)
                                                                         aGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTAgtt                                                        >  1:2704165/1‑126 (MQ=255)
                                                                          gtcaataactaaaaataTTTTCTTTTTTTTTTTTTTTTGTTATTGCAAATTTTTAAAAAATTAAATTTAAATTTTTAAAAATATTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTa                                                       <  1:560977/122‑1 (MQ=255)
                                                                            aaGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATATCTTTTTTTTTTTTTTTTTTATATTTGAAATTAAAtttttatttttt                                                    >  2:625832/1‑120 (MQ=255)
                                                                              taaaataaaaataTTATATTTTTATATATTTTTTTTAATTGCAAATTTTTAAAAAATTAATTTTAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTttg                                                  <  1:2914313/127‑1 (MQ=255)
                                                                               aaactaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTATTATATT‑‑AATTATAATTTTTATTTAttttt                                                  >  1:1807279/1‑127 (MQ=255)
                                                                                 actaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATTATTTAAATATAAATTTTaataattttttt                                                >  2:558698/1‑117 (MQ=255)
                                                                                 actaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTATTTTTTTTTTTTTATAATTTATAAATAAATTTTATGTtttttttt                                                >  2:377257/1‑128 (MQ=255)
                                                                                  ctaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATATCTTTTTTTTTTTTTTTTTTAAAATTAAAAAATAAATTTTattaatatttttt                                               >  2:2319272/1‑116 (MQ=255)
                                                                                  ataaaaattTTATATTTTTATATATTTTTTTTATATGCAAATTTTTAAAAAATTAATTTTAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTc                                             <  1:136242/127‑1 (MQ=255)
                                                                                   taaaacttTTTTGTTTTTTTTTATTTTTTGTTTATGTAAATTTTTAAAAAATTAATTTTTAATTGTAAAAAAT‑‑‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCt                                            <  1:2208199/128‑1 (MQ=255)
                                                                                   taaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTATTTTTTTttttgt                                              >  2:3121901/1‑128 (MQ=255)
                                                                                   taaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTAAATGTAATTAAAAATTTTATGTAATTtgttgta                                             >  1:2510917/1‑127 (MQ=255)
                                                                                      aactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTAAAATTTAAATAAAAATTTTAGTTAtttatttttata                                           >  2:1176303/1‑119 (MQ=255)
                                                                                       actaATATCTTTTTAGATATTTTTTTTATATTTAAATTTTTAAAAAATTTATTTTAACTTTTAAAAAAAATCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTaa                                          <  2:2743066/128‑1 (MQ=255)
                                                                                         tattatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATTTAAATAAAAATTTTAGTTATtttttttttaaatt                                        >  2:2254581/1‑120 (MQ=255)
                                                                                         tattatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATTTAAATTTATGGTATTTTGTTGTATAAGTAt                                      >  1:3074470/1‑128 (MQ=255)
                                                                                           ttatTTTTTTATATTTTTTTTGTTTATTTAAATTTTTAAAAAATTTTTTTTTAATTTTTAAAAAT‑‑TTTTTTTTTTTTTTTTTTTTATAATGGAAATATATATTTTAGGTATTTTGTTGTCTAAGTATc                                     <  1:9030/128‑1 (MQ=255)
                                                                                           ttatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGTTTTTTttttgtaaaattata                                     >  1:2670090/1‑119 (MQ=255)
                                                                                             atCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTAAAATTGAAATATAAATTTTAGGTTTTTTTTTGACTATGTaaat                                    >  1:2987203/1‑125 (MQ=255)
                                                                                                   tttATATATTTTTTGTTTATTCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATATCTTTTTTTTTTTTTTATTTATAATGGAAATATAAATTTTAGGTATTATGTTGTCTAAGTATCTaaaaa                               <  2:1239880/128‑1 (MQ=255)
                                                                                                     tttatatTTTTTTTTAATTAAAATTTTTAAAAAATTAATTTTTAATTTTAAAAAATATTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTaaaaaaa                             <  2:1012551/125‑1 (MQ=255)
                                                                                                         tatTTTTTTTTTTTTCAAATTTTTAAAAAATTAATTTTTAATTTTAAAAAATA‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGaa                        <  2:2670090/128‑1 (MQ=255)
                                                                                                            ttttttTATATTTAAATTTTTAAAAAATTTATTTTAAATTTTAAAAAATATATTTTTTTTTTTTTTTTTTATAATTGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAg                      <  1:72001/128‑1 (MQ=255)
                                                                                                             ttttGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTAAAAATAAAATAAAAATTTTAGTTATTTTGTTTTAAAATTATATAAAAAAATATaattg                     >  2:326880/1‑125 (MQ=255)
                                                                                                                tGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTGTATATGGAAAAATAAAAATTAGTTATTGTTGTGTTAAACTAAATAAAAAAAAAAAAAgttaa                  >  1:2121997/1‑124 (MQ=255)
                                                                                                                tGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTAAAAATAAAAAATAAAATTTTTAAATATTTTTTTAAAAAATTATATAAAAAAATaaataaaaa                   >  1:1558485/1‑120 (MQ=255)
                                                                                                                 gTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATATAAAAAAATTGAAAGGaaaaaa               >  1:1851901/1‑128 (MQ=255)
                                                                                                                  tttttttaaaaTTTTTAAAAAATTAATTTTAAATTTTTAAATATATTTTTTTTTTTTTTTTTTTTTATAATGGTAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGaaa                  <  1:2067217/120‑1 (MQ=255)
                                                                                                                   atatGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATTTAAAAAAAAATTTAAGGTATTTTTTTTTATATTTATATAAAAAAATAAAAATTaaaaataa             >  1:2297022/1‑125 (MQ=255)
                                                                                                                    tatTCAAATTTTTAAAAAATTAATTTTAACTTGTAAAAAATTTCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAat              <  1:3254404/128‑1 (MQ=255)
                                                                                                                     attttaaatttttTAAAAAATTATTTTTAATTTTAAAAAATATCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAat              <  2:2828431/119‑1 (MQ=255)
                                                                                                                     atGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAAAGAAAATAAAAATTTTAGTTTATTTTTTTAATAATTATAAAAAAAAATAAAAAAAAAAAAat              >  1:1061933/1‑128 (MQ=255)
                                                                                                                     atGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTAAAATGAAAATATAAATTTTTTTTTTTTTTTTTTATAATTAAATAAAAAAATAAAAAGAAAAAAata             >  1:836965/1‑128 (MQ=255)
                                                                                                                      tGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTAAAATGGAAAAATAAATTTTAGTTATTTTTTTGTAAAATATTGTAAAAAATTAAAATAAAAAAAatat            >  1:1634601/1‑128 (MQ=255)
                                                                                                                            aTTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTATAATTGAAATATAAATTTTATGTATTTTTTTTTATAATTATATAAAAAAATAGAAAGGAAAAAATATTTTTaaaa    >  1:675938/1‑128 (MQ=255)
                                                                                                                              ttttAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTAGGTATTTTTTTTTCTATGTTTATAAAAAAATATAAAGTAAAAAATATTGTTaaa     >  2:3169373/1‑128 (MQ=255)
                                                                                                                              ttttAAAAAATTAAATTGAAATTTTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTaaa     <  1:2842590/128‑1 (MQ=255)
                                                                                                                                  |                                                                                                                                 
TATTTACTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTAAAAGAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGT  >  CP016816/165091‑165346

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: