BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP016816	32,707	A→T	I1067F (ATT→TTT)	JCVSYN2_00105 →	ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP016816	32,707	0	A	T	99.6%	4402.6 / NA	1392	I1067F (ATT→TTT)	JCVSYN2_00105	ABC transporter permease
Reads supporting (aligned to +/- strand): ref base A (5/1); new base T (681/703); total (687/705)
Fisher's exact test for biased strand distribution p-value = 1.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1456 total aligned reads displayed.

GTTACTCAACCTTTAACTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTATTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACCTAAATCAAATAACATTGA  >  CP016816/32581‑32831

  tcatCAAGCTTTAACTCAGTTTGTTGGATAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATttttta                                                                                                                           <  1:174462/125‑1 (MQ=255)

   aCTCAACCTTTAACTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTaa                                                                                                                          >  2:1158950/1‑128 (MQ=255)

   aCTCAACCTTTAACTCAGTTTGTTGGAGAACATTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTaa                                                                                                                          <  1:1266617/128‑1 (MQ=255)

     tCAACCTTTAACTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTACACTTAATTTTAGGTTATTCTCATTCATTTTATAAAt                                                                                                                        >  2:2470605/1‑128 (MQ=255)

      cAACCTTTAACTCAGATTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATc                                                                                                                       <  2:1589101/128‑1 (MQ=255)

             tAACTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTa                                                                                                                <  2:1674198/128‑1 (MQ=255)

              aaCTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTaa                                                                                                               <  2:3151259/128‑1 (MQ=255)

               aCTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAAc                                                                                                              >  2:2046001/1‑128 (MQ=255)

               aCTCAGATTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAAc                                                                                                              <  1:1615120/128‑1 (MQ=255)

                 tCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACaa                                                                                                            >  2:2351328/1‑128 (MQ=255)

                  cAGTTTTTAGGAGAACTATTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAATCTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAg                                                                                                           <  1:1373129/128‑1 (MQ=255)

                   aGTTTGTTGGAGTACTTTTATAAACTATAGATAAAGATAACGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATATTTGGATATTCTCCTTCATTTTTTAAATCTTATTTAACAAGt                                                                                                          <  1:2044351/128‑1 (MQ=255)

                   aGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGt                                                                                                          >  2:2803215/1‑128 (MQ=255)

                   aGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTATCATTCATTTTTTAAATCTTATTTAAAAAGt                                                                                                          >  1:3008137/1‑128 (MQ=255)

                     tttGTTGGAGAACTTTTAAAAACTAATTTTAAAGATAAAGTGCAAACTACAGCAGAGAGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAgaga                                                                                                        >  1:3248372/1‑128 (MQ=255)

                      ttgttgGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAAATACAGGTGAGTTAAAAGAAAAGATCTTAAACTTAATTTTAGGTAATTCTCATTCATTATTTAAATCTTATTTAACAAGTGaa                                                                                                       >  2:2411774/1‑128 (MQ=255)

                        gtgggAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAAtc                                                                                                     <  1:740052/125‑1 (MQ=255)

                         ttgGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAAtct                                                                                                    >  1:2771707/1‑128 (MQ=255)

                         ttgGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAAtct                                                                                                    >  2:2636064/1‑128 (MQ=255)

                          tgGAGTACTTATAAAAACTATTTTTAAAGATAAAGTGCAAACTAGAGGTGAGTGATAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCATTCATTTTTTAATTCTTATTTAACAAGTGAAtctc                                                                                                   <  2:1899890/128‑1 (MQ=255)

                              gaacagtttaaaaCTTTTTTTAAAGATAAAGTGCAAACTAGAGTTTAGTGAACAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAag                                                                                               <  1:2953421/119‑1 (MQ=255)

                               aaCTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAaga                                                                                              >  1:1810154/1‑128 (MQ=255)

                                aCTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAagag                                                                                             <  2:2521224/128‑1 (MQ=255)

                                aCTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAagag                                                                                             >  2:1857095/1‑128 (MQ=255)

                                 cTTTTAAAATCTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGc                                                                                            <  2:400550/128‑1 (MQ=255)

                                 cTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGc                                                                                            >  2:735938/1‑128 (MQ=255)

                                  ttttAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCt                                                                                           <  2:3320115/128‑1 (MQ=255)

                                   tttAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTc                                                                                          <  2:2763896/128‑1 (MQ=255)

                                     tAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGAGAATCTCTAAGAGCTCaa                                                                                        >  2:2322653/1‑128 (MQ=255)

                                       aaaaCTATTTTTAAATATTAATTGCAAACTACAGGTGATTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAAtt                                                                                      <  2:2978357/128‑1 (MQ=255)

                                        aaaCTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAAttt                                                                                     >  2:570906/1‑128 (MQ=255)

                                           cTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTATCAAGTGAATCTCGCTGAGCTCAATTTTca                                                                                  >  2:692797/1‑127 (MQ=255)

                                            tATTTTTAAATATAAAGAGCAAACTACAGTTGAGTGAAAAGAATAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATATTTTAATTCTTATTTAACAAGTGAATCTCGAATAGCTCAATTTTCtt                                                                                 <  2:304391/128‑1 (MQ=255)

                                             aTTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCttt                                                                                <  1:402422/128‑1 (MQ=255)

                                                tttAAAGATAAAGTGCACACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTgg                                                                             <  1:1303958/128‑1 (MQ=255)

                                                tttAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTTAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTgg                                                                             <  1:229621/128‑1 (MQ=255)

                                                tttAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTgg                                                                             <  1:994047/128‑1 (MQ=255)

                                                 ttAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTACCAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGt                                                                            >  2:1115188/1‑128 (MQ=255)

                                                  tAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGtt                                                                           >  1:2844494/1‑128 (MQ=255)

                                                  tAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGtt                                                                           >  2:1494881/1‑128 (MQ=255)

                                                        tAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACaaa                                                                     <  2:3268069/128‑1 (MQ=255)

                                                          aaGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACaaaaa                                                                   >  1:3326204/1‑128 (MQ=255)

                                                           aGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAaaac                                                                  >  1:1083058/1‑128 (MQ=255)

                                                           aGTGCAAACAACAGGGGTTTGAAAATAAAATATCTTAAAATTAATTTAAGGTTATTTTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAaaac                                                                  <  1:2232807/128‑1 (MQ=255)

                                                            ttGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAaaaca                                                                 <  1:616612/127‑1 (MQ=255)

                                                              gCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAaaacaaa                                                               >  2:1227723/1‑128 (MQ=255)

                                                               cAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAaaacaaac                                                              >  1:1697630/1‑128 (MQ=255)

                                                                 aaCTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACta                                                            >  2:1079559/1‑128 (MQ=255)

                                                                 aaCTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACta                                                            >  1:885304/1‑128 (MQ=255)

                                                                    tACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATaa                                                         >  1:142683/1‑128 (MQ=255)

                                                                       aGGTGAGTGAAAAGAAAAGATCGTAAACTTAATTTTAGGTTAGTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTc                                                      <  1:677218/128‑1 (MQ=255)

                                                                        ggTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTcc                                                     <  2:1827105/128‑1 (MQ=255)

                                                                        ggTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTACTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTcc                                                     <  1:1007994/128‑1 (MQ=255)

                                                                          tgagtgaAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCcac                                                   >  2:1780827/1‑128 (MQ=255)

                                                                           gagtgaAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCcaca                                                  >  2:3085785/1‑128 (MQ=255)

                                                                           gagtgaAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCATATTTAACAAGTGAATCTCTAAGAGCTAAATTTTATTTTGGTTGACTAAAACAAACTATAATTCcaca                                                  >  1:319249/1‑128 (MQ=255)

                                                                            agtgaAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACaa                                                 <  1:2613862/128‑1 (MQ=255)

                                                                             gtgaAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACaaa                                                >  1:1466747/1‑128 (MQ=255)

                                                                             gtgaAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACaaa                                                >  1:845487/1‑128 (MQ=255)

                                                                                aaaagaaaagATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAg                                             >  2:514263/1‑128 (MQ=255)

                                                                                  aataaaagaTCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACATAAAGAt                                           >  1:1950640/4‑128 (MQ=255)

                                                                                    gaaaagaTCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCa                                         <  1:511833/128‑1 (MQ=255)

                                                                                     aaaagaTCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCaa                                        >  2:2849676/1‑128 (MQ=255)

                                                                                     aaaagaTCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCaa                                        <  1:2687188/128‑1 (MQ=255)

                                                                                      aaagaTCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGCTCAAt                                       >  1:58330/1‑128 (MQ=255)

                                                                                       aagaTCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAAATCAAtt                                      >  1:2520552/1‑128 (MQ=255)

                                                                                       aagaTCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCAACAAAAAGATAAAtt                                      >  2:1717073/1‑128 (MQ=255)

                                                                                         gaTCTTAAACTTCATGTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAg                                    <  1:2471655/128‑1 (MQ=255)

                                                                                         gaTCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAg                                    <  1:936735/128‑1 (MQ=255)

                                                                                         gaTATTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGAGAATCTCGAAGAGCTCAATTTTCTTTTTGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAg                                    <  2:1362178/128‑1 (MQ=255)

                                                                                            cTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAATTGAATATCGAAGAGATCAATTTTCTTTTTTTTGACAAATAAAAACTATAAATACACAAAAATATCAATTAGCaa                                 >  2:79037/1‑128 (MQ=255)

                                                                                            cTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCaa                                 >  1:1118235/1‑128 (MQ=255)

                                                                                            cTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCaa                                 >  2:3197771/1‑128 (MQ=255)

                                                                                              tAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACt                               <  2:1924523/128‑1 (MQ=255)

                                                                                              tAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACt                               >  1:1855812/1‑128 (MQ=255)

                                                                                              tAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACt                               >  1:1896109/1‑128 (MQ=255)

                                                                                              tAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACt                               <  1:242343/128‑1 (MQ=255)

                                                                                                aaCTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTAt                             <  2:932001/128‑1 (MQ=255)

                                                                                                aaCTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTAt                             >  1:211904/1‑128 (MQ=255)

                                                                                                 aCTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTAtt                            >  2:1549128/1‑128 (MQ=255)

                                                                                                 aCTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTAtt                            <  2:632576/128‑1 (MQ=255)

                                                                                                   ttAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTAtttt                          <  1:802794/128‑1 (MQ=255)

                                                                                                    tAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTAttttt                         <  2:869835/128‑1 (MQ=255)

                                                                                                    tAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTAttttt                         >  2:1313210/1‑128 (MQ=255)

                                                                                                    tAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTAttttt                         <  2:2698503/128‑1 (MQ=255)

                                                                                                    tAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTAttttt                         <  1:3072211/128‑1 (MQ=255)

                                                                                                    tAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTAtttta                         >  2:460811/1‑127 (MQ=255)

                                                                                                    tAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTAGAAGAGATCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTAttttt                         <  2:1455381/128‑1 (MQ=255)

                                                                                                     aaTTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTa                        >  1:2353859/1‑128 (MQ=255)

                                                                                                        tttAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAAc                     <  2:2268485/128‑1 (MQ=255)

                                                                                                           aGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACCTa                  <  1:2884514/128‑1 (MQ=255)

                                                                                                             gTTATTCTCCTTCATTTTTTAAATCTTATATAACAAGTGAATCACGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACCTaaa                <  2:2126186/128‑1 (MQ=255)

                                                                                                                 ttCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACATAAACAAACTATAATTCCACATAAAGATCAATTAGCAACTATTTTTAAACCTAAATCaa            >  1:1543691/1‑128 (MQ=255)

                                                                                                                 ttCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACCTAAATCaa            >  2:219303/1‑128 (MQ=255)

                                                                                                                    tcCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACCTAAATCAAATa         <  1:118628/128‑1 (MQ=255)

                                                                                                                       ttCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACCTAAATCAAATAACa      >  2:1992853/1‑128 (MQ=255)

                                                                                                                       ttCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACAATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACCTAAATCAAATAACa      <  1:3147523/128‑1 (MQ=255)

                                                                                                                           ttttttAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACCTAAATCAAATAACATTGa  >  1:467363/1‑128 (MQ=255)

                                                                                                                           ttttttAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACCTAAATCAAATAACATTGa  >  2:2938976/1‑128 (MQ=255)

                                                                                                                           ttttttAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTATAAACCTAAATCAAATAACATTGa  >  1:2181634/1‑128 (MQ=255)

GTTACTCAACCTTTAACTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTATTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACCTAAATCAAATAACATTGA  >  CP016816/32581‑32831

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`