BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP016816	420,163	2 bp→TT	intergenic (‑446/+396)	JCVSYN2_01895 ← / ← JCVSYN2_01900	30S ribosomal protein S10/tRNA‑Thr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP016816	420,163	0	A	T	99.5%	3974.5 / NA	1309	intergenic (‑446/+397)	JCVSYN2_01895/JCVSYN2_01900	30S ribosomal protein S10/tRNA‑Thr
Reads supporting (aligned to +/- strand): ref base A (2/5); new base T (676/622); total (679/630)
Fisher's exact test for biased strand distribution p-value = 2.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	CP016816	420,164	0	A	T	98.5%	3508.9 / NA	1288	intergenic (‑447/+396)	JCVSYN2_01895/JCVSYN2_01900	30S ribosomal protein S10/tRNA‑Thr
Reads supporting (aligned to +/- strand): ref base A (2/17); new base T (665/603); total (667/621)
Fisher's exact test for biased strand distribution p-value = 2.52e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1311 total aligned reads displayed.

CATTCATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTAAAATTAAAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGC‑T‑C‑G‑‑TTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTAAACGTATATT  >  CP016816/420041‑420285

||

cattcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATa                                                                                                                            <  2:1756440/128‑1 (MQ=255)

cattcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATa                                                                                                                            <  2:2109176/128‑1 (MQ=255)

   tcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACt                                                                                                                         >  2:583115/1‑128 (MQ=255)

      tACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGaa                                                                                                                      <  1:3091280/128‑1 (MQ=255)

      tACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGaa                                                                                                                      <  2:339049/128‑1 (MQ=255)

       aCTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAat                                                                                                                     >  1:2342784/1‑128 (MQ=255)

        cTTTTAGTCTAATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAatt                                                                                                                    <  2:15883/128‑1 (MQ=255)

         ttttAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAatta                                                                                                                   <  1:610805/128‑1 (MQ=255)

          tttAGTCTCATAAATCTAAGTGTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTAATAGTTGATATAGTAGAAAACTACTATCAATTTTTTAAAAAATTATAAATATTAATAAATGAataag                                                                                                                  >  2:1182685/1‑125 (MQ=255)

           ttAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTTATATATTAGAAAACTACTAGCACATTTTTAAAAAATTTTTAATTTTAATAAAATAattatt                                                                                                                 >  2:701532/1‑128 (MQ=255)

           ttAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAattatt                                                                                                                 <  1:3485839/128‑1 (MQ=255)

           ttAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAattatt                                                                                                                 <  1:748375/128‑1 (MQ=255)

               tctcATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTc                                                                                                             >  2:3685904/1‑128 (MQ=255)

                  cATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCaaa                                                                                                          >  1:2271267/1‑128 (MQ=255)

                  cATAAATCTAAGTTTCTAAAATTATTTAATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCaaa                                                                                                          <  1:528342/128‑1 (MQ=255)

                   aTAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAAt                                                                                                         <  1:3657566/128‑1 (MQ=255)

                   aTAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAAt                                                                                                         >  2:3835515/1‑128 (MQ=255)

                   aTAAATCTAAGGTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAAt                                                                                                         <  2:3505433/128‑1 (MQ=255)

                      aaTCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGt                                                                                                      >  2:2270896/1‑128 (MQ=255)

                       aTCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGtt                                                                                                     >  1:395778/1‑128 (MQ=255)

                       aTCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGtt                                                                                                     >  2:855156/1‑128 (MQ=255)

                       aTCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGtt                                                                                                     >  1:3280265/1‑128 (MQ=255)

                       aTCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGtt                                                                                                     >  1:2868284/1‑128 (MQ=255)

                       aTCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACAGAATTATTTTTCAAATTGtt                                                                                                     >  2:1812219/1‑128 (MQ=255)

                        tCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTa                                                                                                    <  2:197248/128‑1 (MQ=255)

                         cTAAGTTTATAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTaa                                                                                                   <  2:2798398/128‑1 (MQ=255)

                           aaGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAat                                                                                                 <  2:1392527/128‑1 (MQ=255)

                            aGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAatt                                                                                                <  2:2963951/128‑1 (MQ=255)

                            aGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAAATAATTTTCAAATTGTTaaaat                                                                                                >  1:1619149/1‑126 (MQ=255)

                               ttCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAattatt                                                                                             >  2:3621965/1‑128 (MQ=255)

                                tCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCaa                                                                                                           <  2:269359/113‑1 (MQ=255)

                                tCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTCAATTATTTTTCAAATTGTTAAATTAttt                                                                                            >  1:886858/1‑128 (MQ=255)

                                 cTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAtttt                                                                                           >  1:3328922/1‑128 (MQ=255)

                                  tAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTc                                                                                          <  1:1975812/128‑1 (MQ=255)

                                   aaaaTTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCa                                                                                         <  2:438160/128‑1 (MQ=255)

                                   aaaaTTATTTTATAATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCa                                                                                         <  2:1315903/128‑1 (MQ=255)

                                     aaTTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTATCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTTTTAAATTATTTTaaaa                                                                                       >  1:2519080/1‑128 (MQ=255)

                                      attattTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAAt                                                                                      <  1:1799137/128‑1 (MQ=255)

                                      attattTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAAt                                                                                      >  1:1377604/1‑128 (MQ=255)

                                       ttattTTGTCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATc                                                                                     >  1:1316766/1‑128 (MQ=255)

                                        tattTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTAttttt                                                                                                              >  2:2215425/1‑102 (MQ=255)

                                            ttATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTAt                                                                                <  2:393224/128‑1 (MQ=255)

                                             tATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTATCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATc                                                                               <  1:263184/128‑1 (MQ=255)

                                              aTCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCt                                                                              <  1:2939357/128‑1 (MQ=255)

                                               tCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTa                                                                             <  1:2574161/128‑1 (MQ=255)

                                                 aTTTAAAAAAATAATACTTTTATTAGTTGATATATTTGAAAACTACTAGCACTTTTTTAAAAAATTTTTATTTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTAtt                                                                           >  1:1877522/1‑128 (MQ=255)

                                                   ttAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTaa                                                                         >  1:1009990/1‑128 (MQ=255)

                                                    tAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTATCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAAt                                                                        <  2:3556759/128‑1 (MQ=255)

                                                        aaaaTAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAAATGTTAAATTATTTTCAAATCATTATCTATTAATCAAt                                                                    >  2:2874492/1‑128 (MQ=255)

                                                         taataatACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAAtt                                                                   <  1:2769935/127‑1 (MQ=255)

                                                          aataatACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAAttt                                                                  <  1:1454113/128‑1 (MQ=255)

                                                           ataataCTTTTATTATTAGATATATTATAAAACTACTATCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTAACTATTAATCAAtttt                                                                 <  2:2578718/128‑1 (MQ=255)

                                                           ataataCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAAtttt                                                                 >  2:958051/1‑128 (MQ=255)

                                                              ataCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAATTTCAAATCATTATCTATTAATCAATTTTATg                                                              >  1:2716533/1‑128 (MQ=255)

                                                               taCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGc                                                             >  1:3151855/1‑128 (MQ=255)

                                                                aCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGC‑t                                                           >  2:309688/1‑128 (MQ=255)

                                                                aCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGC‑t                                                           >  2:779654/1‑128 (MQ=255)

                                                                 cTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGC‑T‑c                                                         >  1:3601843/1‑128 (MQ=255)

                                                                    ttattaGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGC‑T‑C‑G‑‑tt                                                   >  1:3528584/1‑128 (MQ=255)

                                                                     tattaGTTTTTATATTATAAAACTACTATCAATTTTTTAAAAAATTTTTAATTTTAAAAACTTTATTATTTTTAAAATTTTTAAATTTTTTTCAAATCATTATCTATTAATCAATTTTATTC‑T‑C‑G‑‑ttt                                                  <  2:3008143/128‑1 (MQ=255)

                                                                     tattaGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGC‑T‑C‑G‑‑ttt                                                  >  2:234464/1‑128 (MQ=255)

                                                                     tattaGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGC‑T‑C‑G‑‑ttt                                                  >  1:3228103/1‑128 (MQ=255)

                                                                     tattaGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGC‑T‑C‑G‑‑ttt                                                  >  2:1103475/1‑128 (MQ=255)

                                                                     tattaGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAAAAAATTTTATTC‑T‑C‑G‑‑ttt                                                  >  2:3872467/1‑128 (MQ=255)

                                                                      attattTGATATATTAGAAAAATTCTATCAATTTTTTAAAAAATTTTTAAATTTAATAACTGAATTATTTTTAAAATTTTTAAATTATTTTCAAATCATTATCAATTAATCAAATTTATGC‑G‑C‑G‑‑tttt                                                 <  1:2115547/128‑1 (MQ=255)

                                                                         atttGATTTATTAGAAAACTAATAGCAATTTTTTAAAAAATTTTTAATTTTAATAACAGAATTATTTTTCAAATTTTTAAATTATTTTCAAATCATTATCAATAAATCAATTTTATGC‑T‑C‑G‑‑ttttttt                                              <  1:3900017/126‑1 (MQ=255)

                                                                           ttGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGC‑T‑C‑G‑‑ttttttttt                                            >  2:3767370/1‑128 (MQ=255)

                                                                                atatTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATTC‑T‑C‑G‑‑tttttttttttttt                                       >  1:1546249/1‑128 (MQ=255)

                                                                                atatTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATAATTATCTATTAATCAATATTATGC‑T‑C‑G‑‑tttttttttttttt                                       >  2:2479691/1‑128 (MQ=255)

                                                                                atatTAGAAAACTACTAGCACTTTTTTAAAAAATTTATAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGC‑T‑C‑G‑‑tttttttttttttt                                       >  1:3855408/1‑128 (MQ=255)

                                                                                 ttttATAAAACTTCTAGCACTTTTTTAAAAAATTTATAAATATAATAACTGAATTATTTTTAAAATTGTTAAATTTTTTTCAAATCAATATCTATTAATCAATTTTTTGC‑T‑C‑G‑‑ttttttttttttttt                                      <  1:977459/126‑1 (MQ=255)

                                                                                 taatAGAAAACTAATATAACTTTTTTAAAAAATTTTTAATTTTAATAAATTAATTATTTTTAAAATTGTTAAATTATTTTCAAATATTTATATATTAATCAATTTTATGC‑T‑C‑G‑‑tattttttttttttt                                      <  1:1294272/125‑1 (MQ=255)

                                                                                     aGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGC‑T‑C‑G‑‑ttttttttttttttttaaa                                  >  1:2216906/1‑125 (MQ=255)

                                                                                      gAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGC‑T‑C‑G‑‑TTTTTTTTTTTTTTTTTaaa                                 >  2:1508034/1‑126 (MQ=255)

                                                                                        aaaCTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGC‑T‑C‑t‑‑tttttttttttttttaaaaaaa                               >  1:1682903/1‑121 (MQ=255)

                                                                                        aaaCTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGC‑T‑C‑T‑‑TTTTTTTTTTTTTTTTTAaaaa                               >  1:2438447/1‑128 (MQ=255)

                                                                                        aaaCTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGC‑T‑C‑G‑‑TTTTTTTTTTTTTTTTAACaaa                               >  2:3229191/1‑128 (MQ=255)

                                                                                          actactAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGC‑T‑C‑G‑‑TTTTTTTTTTTTTTTTTACaaaaa                             >  2:2024840/1‑128 (MQ=255)

                                                                                          actactAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGC‑T‑C‑G‑‑TTTTTTTTTTTTTTTTTACaaaaa                             >  1:3735601/1‑128 (MQ=255)

                                                                                           ctactaGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGC‑T‑C‑G‑‑‑‑TTTTTTTTTTTTTTTACAAAAatat                          >  1:2186092/1‑128 (MQ=255)

                                                                                            tactaGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTAtt                                                                           <  1:3418618/85‑1 (MQ=255)

                                                                                            tactaGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGC‑T‑C‑G‑‑TATTTTATATTTTTTTAAAAAAAata                           >  1:1745895/1‑128 (MQ=255)

                                                                                             tatatgaattttttAAAAAATTTTTAATTTTAATAAATGAATTTATTTTAAAATTTTTAAATTATTTTCAAATTATTATATATTAATAAATTTTATGC‑T‑C‑GT‑TTTTTTTTTTTTTTTTTACAAAAata                           <  1:3062050/120‑1 (MQ=255)

                                                                                               tataaattttttAAATAATTTTTAATTTTAATAAATTAATTATTTTTCAATTTGTTAAATTATTTTTAAATCATTAACAATTTATCAATTTTTTG‑‑T‑T‑G‑‑GTTTTTTTTTTTTTTTTACAAAAATATAat                       <  2:1264437/122‑1 (MQ=255)

                                                                                                 gCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATTA‑T‑A‑G‑‑TTTTTTTTTTTTTTTTTAAAAAAATATAatt                      >  1:1220730/1‑128 (MQ=255)

                                                                                                  cACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGC‑T‑CTT‑‑TTTTTTTTTTTTTTTTTAAAAAAAAATAatt                      >  1:694601/1‑128 (MQ=255)

                                                                                                  cACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGC‑T‑C‑GTTTTTTTTTTTTTTTTTTTAAAAAAATATAat                       >  1:1144895/1‑128 (MQ=255)

                                                                                                  cACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGC‑T‑C‑G‑‑‑TTTTTTTTTTTTTTTAAAAAAAATATAtttta                    >  1:455109/1‑128 (MQ=255)

                                                                                                  cACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATAGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGC‑T‑C‑G‑‑‑TATTTTTTTTTTTTTTAAATAAATATAtttta                    >  1:3785916/1‑128 (MQ=255)

                                                                                                    tatttttAAAAAATTTTTAATTTTTATAAATTAATTATTTTTTAAATTTTTAATTTATTTTAAAATAATTTTATATTAATAAATTTTTTTC‑TAT‑T‑‑TTTTTTTTTTTTTTTTTACAAAAATATAattta                    <  1:2017600/126‑1 (MQ=255)

                                                                                                     ttttttAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGC‑T‑C‑G‑‑TTTTTTTTTTTTTTTTTAAAAAAAAATaaaattat                  >  2:1025285/1‑122 (MQ=255)

                                                                                                       ttttAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGC‑T‑C‑G‑‑TTTTTTTTTTTTTTTTTAAAAAATAAAAatttaattt                >  2:2627335/1‑127 (MQ=255)

                                                                                                        tttAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGC‑T‑C‑GT‑TTTTTTTTTTTTTTTTTAAAAAAAAATAatttttttt                >  2:3155954/1‑127 (MQ=255)

                                                                                                        tttAAAAAATTTTTAAATTTAATAACTGAATTAATTTTCAAATATTTTAATTATTTTCAAATCATTATATATTAATCAAATTTATGC‑T‑C‑G‑‑TTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGa               <  2:488418/128‑1 (MQ=255)

                                                                                                         ttAAAAAATTTTTAATTTTAATAACTTAATTATTTTTAAAATTGTTAAATTATTTTCAAATCATTATATTTTAATCAATTTTATGT‑T‑C‑G‑‑‑TTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGAtt             <  2:3305899/128‑1 (MQ=255)

                                                                                                           aaaaaaTTTTTAAATTTAATAACTGTATTATTTTTTAAATTGTTAATATATTTTTAAATTATTATTTATTAATAAATTTTATGCTT‑T‑T‑‑TTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGAtt             <  2:1692054/128‑1 (MQ=255)

                                                                                                            aaaaaTTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGC‑T‑C‑GT‑TTTTTTTTTTTTTTTTTAAAAAAATAAAatttattatataa            >  2:1903121/1‑122 (MQ=255)

                                                                                                             aaaaTTTTTAATTTTAATAAATTTATTATTTTTTAAATTTTTTAATTATTTTTAAATAATTATAATTTAATTAATTTTATTA‑T‑A‑T‑‑TTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTaaa          <  1:1081927/128‑1 (MQ=255)

                                                                                                               aaTTTTTAATTTTAATAAATTAATTATTTTTTAAATTTTTTAATTATTTTAAAATAATTATATATTAATAAATTTTATTT‑T‑C‑‑‑‑TTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTAAAcat       <  1:799998/128‑3 (MQ=255)

                                                                                                                 tttttaatttaaatAACTGAATAATTTTTCAAATTGTTAAATTATTTTCAAATCATTATATATAAATCAATTTTATGC‑T‑C‑G‑‑TTTTTTTTTTTTTTTTAAAAAAAAAATAATTTATTTTATATAACGta      >  1:2991971/6‑128 (MQ=255)

||

CATTCATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTAAAATTAAAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGC‑T‑C‑G‑‑TTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTAAACGTATATT  >  CP016816/420041‑420285

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`