Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 20,023 2 bp→TA intergenic (‑744/‑392) JCVSYN2_00065 ← / → JCVSYN2_00070 hypothetical protein/tetracycline resistance ribosomal protection protein Tet(M)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP01681620,0230AT99.7% 4132.1 / NA 1417intergenic (‑744/‑393)JCVSYN2_00065/JCVSYN2_00070hypothetical protein/tetracycline resistance ribosomal protection protein Tet(M)
Reads supporting (aligned to +/- strand):  ref base A (1/3);  new base T (755/657);  total (756/661)
Fisher's exact test for biased strand distribution p-value = 3.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP01681620,0240TA99.8% 4271.2 / NA 1417intergenic (‑745/‑392)JCVSYN2_00065/JCVSYN2_00070hypothetical protein/tetracycline resistance ribosomal protection protein Tet(M)
Reads supporting (aligned to +/- strand):  ref base T (2/1);  new base A (754/660);  total (756/661)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1450 total aligned reads displayed.

AGAGCAGGAAGAGCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATAATTTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGAAAAAACAAAAAAAG  >  CP016816/19898‑20145
                                                                                                                             ||                                                                                                                         
   gCAGGAAGAGCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTta                                                                                                                       >  2:2182178/1‑128 (MQ=255)
   gCAGGAAGAGCAAGATAAAAGGTAGTATTTGTTGGCGATCACCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTta                                                                                                                       >  2:2398492/1‑128 (MQ=255)
      ggAAGAGCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTTTTTTTTCTTTAATTTCTTTTTTTACGTTCTATTTTTAatttatatatttatat                                                                                                                    >  2:593786/1‑128 (MQ=255)
        aaGAGCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTATTTACTTTCTATTTTTAATTTATATATTTATATTa                                                                                                                  >  2:2035420/1‑128 (MQ=255)
        aaGAGCAAGATAAAAGGTAGTAGTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTAGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTa                                                                                                                  <  2:2308608/128‑1 (MQ=255)
           agCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTaaaa                                                                                                               >  2:1466824/1‑128 (MQ=255)
           agCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTaaaa                                                                                                               >  2:3431097/1‑128 (MQ=255)
              aaGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAAt                                                                                                            >  2:1093426/1‑128 (MQ=255)
              aaGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATATTTTCTTTAATTTCTTTTGTTACTGTCTAGTATTAATTTATATATTTAGATTAATAAAt                                                                                                            >  1:2526158/1‑128 (MQ=255)
                   aaaaGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTaaa                                                                                                       <  2:3893661/128‑1 (MQ=255)
                   aaaaGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTaaa                                                                                                       <  2:510784/128‑1 (MQ=255)
                   aaaaGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTGCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTAGTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTaaa                                                                                                       >  1:3641811/1‑128 (MQ=255)
                   aaaaGGTAGTATTTGTAGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTaaa                                                                                                       <  2:1764651/128‑1 (MQ=255)
                    aaaGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAaat                                                                                                      <  2:3138580/128‑1 (MQ=255)
                     aaGGTAGTATTTGTTGGCGATCCCGCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAaatt                                                                                                     <  2:3951644/128‑1 (MQ=255)
                     aaGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTaaa                                                                                                                <  2:2791093/117‑1 (MQ=255)
                      aGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTaaaaa                                                                                                              <  1:1584781/118‑1 (MQ=255)
                        gtagtaTTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAaattata                                                                                                  <  2:729784/128‑1 (MQ=255)
                         tagtaTTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAaattataa                                                                                                 <  2:439984/128‑1 (MQ=255)
                          agtaTTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAaattataat                                                                                                >  1:3558065/1‑128 (MQ=255)
                          agtaTTTGTTGGCGATCACCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAaattataat                                                                                                <  1:2831752/128‑1 (MQ=255)
                           gtaTTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTtat                                                                                                                      <  2:3769404/105‑1 (MQ=255)
                             aTTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAaattataattat                                                                                             >  2:3017327/1‑128 (MQ=255)
                              tttGTTGGAGATACCCCTAGAGTCTTTTACATCTTCTGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAattatt                                                                                            <  1:765714/128‑1 (MQ=255)
                               ttgttgGCGATCCCCCTAGAGTCTTTTACATCTTCGGATAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTAttt                                                                                           >  1:3505340/1‑128 (MQ=255)
                                     gCGATCCCCCTAGAGTCGTTTACATCTTCGGAAAACAAAAACTATATTTTCTTTAATTTCTATTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAg                                                                                     >  1:3217448/1‑128 (MQ=255)
                                      cgctcgcccTAGACTCTGTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGc                                                                                    <  2:1032936/122‑1 (MQ=255)
                                      cGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGc                                                                                    <  1:1892390/128‑1 (MQ=255)
                                        aTCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCAc                                                                                  >  1:1200869/1‑128 (MQ=255)
                                         tCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAaattataatta                                                                                              >  2:661093/1‑115 (MQ=255)
                                           gcacTAGAGTCTTTTTCATCTTTTGAAAACAAAATCTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTATATAGCACGTg                                                                               <  1:827942/125‑1 (MQ=255)
                                           ccccTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTg                                                                               <  2:931502/128‑1 (MQ=255)
                                           ccacTAGATGCTTTTACATCTTCGGAAATCTAAAACAATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTg                                                                               <  2:1205883/125‑1 (MQ=255)
                                             ccTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGAt                                                                             <  1:1755867/128‑1 (MQ=255)
                                               tAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATaa                                                                           >  1:266408/1‑128 (MQ=255)
                                                agagTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAAt                                                                          >  2:3626011/1‑128 (MQ=255)
                                                agagTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAAt                                                                          <  1:246246/128‑1 (MQ=255)
                                                  agTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTa                                                                        >  2:3300984/1‑128 (MQ=255)
                                                   gTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTaa                                                                       >  2:224756/1‑128 (MQ=255)
                                                   gTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTaa                                                                       >  1:568173/1‑128 (MQ=255)
                                                     cTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTaaaa                                                                     >  2:863829/1‑128 (MQ=255)
                                                      ttttACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAg                                                                    >  2:1951656/1‑128 (MQ=255)
                                                         tACATCTTCTGAAAAAAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTa                                                                 <  1:899658/128‑1 (MQ=255)
                                                          aCATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAg                                                                <  2:162297/128‑1 (MQ=255)
                                                          aCATCTTCGGAAAACAAAAACTATTTATTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAg                                                                <  1:3844606/128‑1 (MQ=255)
                                                              cTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGaa                                                            <  1:1825554/128‑1 (MQ=255)
                                                              cTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGaa                                                            >  2:563104/1‑128 (MQ=255)
                                                                    aaaaCAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGaa                                                      <  1:240866/128‑1 (MQ=255)
                                                                    aaaaCAAAAACTATTATTTCTTAAATTTCATTATTTACTTTCTATTTTTAATTTTTATATTTATATTAAAAATTTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGaa                                                      <  2:2093139/128‑1 (MQ=255)
                                                                         aaaaaCTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAg                                                 <  1:1261898/128‑1 (MQ=255)
                                                                          aaaaCTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGt                                                <  2:370163/128‑1 (MQ=255)
                                                                           taaCTATTTTTTCTTTAATTTCTTTTTTTACTTTATATTTTTAATTTTTATATTTATATTAAAAAAATTAAATTTTAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAACCAGTg                                               <  2:2486107/127‑1 (MQ=255)
                                                                           ataatATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTg                                               <  1:3060058/124‑1 (MQ=255)
                                                                           aaaaTATTTTTTATTTTATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAATTTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTg                                               <  2:3070295/128‑1 (MQ=255)
                                                                           aaaCTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTg                                               <  2:3474461/128‑1 (MQ=255)
                                                                            aaCTATTTTTTCTTTAATTTCTTTTTTTTCTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGAACTTGATATTTAAAAGTTAGTGAAAAAGAAAACAGTGa                                              >  1:3899927/1‑128 (MQ=255)
                                                                            aaCTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGAt                                                                             >  1:3316093/1‑97 (MQ=255)
                                                                            aaCTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGa                                              <  2:3384693/128‑1 (MQ=255)
                                                                             aCTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTaaa                                             >  2:2520147/1‑128 (MQ=255)
                                                                              cTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAATTTAGTGAACAAGAAAACAGTGAAg                                            >  2:2658774/1‑128 (MQ=255)
                                                                              cTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGaaactggtgctt                                            <  1:2917089/128‑10 (MQ=255)
                                                                              cTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAg                                            <  1:1520069/128‑1 (MQ=255)
                                                                              cTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAg                                            >  1:1006714/1‑128 (MQ=255)
                                                                               tATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGc                                           <  2:2946656/128‑1 (MQ=255)
                                                                               tATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGc                                           >  1:2148018/1‑128 (MQ=255)
                                                                                aTTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCa                                          >  1:657485/1‑128 (MQ=255)
                                                                                aTTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCa                                          >  1:2592891/1‑128 (MQ=255)
                                                                                 ttttttCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCAc                                         >  2:355405/1‑128 (MQ=255)
                                                                                  tttttCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTAAACAAGAAAACAGTGAAGCAcc                                        >  2:2867521/1‑128 (MQ=255)
                                                                                    tttCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAg                                      <  2:320271/128‑1 (MQ=255)
                                                                                    tttCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAg                                      <  2:1720506/128‑1 (MQ=255)
                                                                                       cTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAATAAATTTAAATTATAATTTTTTTTATAGCAAGTGATATTAATTAGTTAGTGAAAAAGAAAACAGTGATGCTCAAGttt                                   >  2:1528193/1‑128 (MQ=255)
                                                                                        tttAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTc                                  >  1:1424836/1‑128 (MQ=255)
                                                                                        tttAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTc                                  <  2:1267311/128‑1 (MQ=255)
                                                                                        tttAATTTCTATTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGAACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTc                                  <  1:985918/128‑1 (MQ=255)
                                                                                          tAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCTCGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTg                                <  2:1348922/128‑1 (MQ=255)
                                                                                             tttCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGaa                                                            <  1:3448791/97‑1 (MQ=255)
                                                                                             tttCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAAc                             >  1:7205/1‑128 (MQ=255)
                                                                                             tttCTTATTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAAc                             <  2:2730218/128‑1 (MQ=255)
                                                                                              ttCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAAcc                            <  1:2035420/128‑1 (MQ=255)
                                                                                                 tttttttACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCaaa                         <  1:2291533/128‑1 (MQ=255)
                                                                                                   tttttACTTTCTATTTTTAATTTTTATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAaag                       <  2:777437/128‑1 (MQ=255)
                                                                                                      ttACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGGACAAGAAAACAGTGAAGAACCAGTTTCTGAAACATatgaag                    >  1:1701883/1‑128 (MQ=255)
                                                                                                      ttACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAaagaag                    >  2:256637/1‑128 (MQ=255)
                                                                                                         cTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACg                 >  2:3850913/1‑128 (MQ=255)
                                                                                                          tttCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGa                >  1:313227/1‑128 (MQ=255)
                                                                                                           ttCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGaa               <  1:1089337/128‑1 (MQ=255)
                                                                                                           ttCTATTTTTAATTTATATATATATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGaa               <  1:2753574/128‑1 (MQ=255)
                                                                                                             cTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGaaaa             <  1:2089499/128‑1 (MQ=255)
                                                                                                             cTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGaaaa             >  1:296960/1‑128 (MQ=255)
                                                                                                             cTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGaaaa             >  1:411332/1‑128 (MQ=255)
                                                                                                              tATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAATAAAACAGTTAAGCACCAGTTTCTGCACCAAAAGAAGACTaaaaa            >  2:1828237/1‑128 (MQ=255)
                                                                                                              tATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGaaaaa            >  1:3746546/1‑128 (MQ=255)
                                                                                                               aTTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGaaaaaa           <  1:3398317/128‑1 (MQ=255)
                                                                                                                tttttAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCAACAGTTTCTGAACCAAAAGAAGACGAAAAAAc          <  1:617527/128‑1 (MQ=255)
                                                                                                                     aaTTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGAAAAAACaaaaa     >  1:1375869/1‑128 (MQ=255)
                                                                                                                      atttatatatttatatTAAAAAATTTAAATTATAATTATTTTTATAGCACTTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGAACAAGTTTCTGAACCAAAAGAATACTAATaaaaaaaaaa    >  2:1899219/1‑128 (MQ=255)
                                                                                                                      atttatatatttatatTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGAAAAAACaaaaaa    >  1:473749/1‑128 (MQ=255)
                                                                                                                       tttATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGAAAAAACaaaaaaa   >  2:1389312/1‑128 (MQ=255)
                                                                                                                        ttatatttttatatttAAATATTTAAATTATAATTATTTTTTTAGTACTTGATAATAAATTGTTAGTGAACAAGAAAACACTGAAGGACCATTTTCTGTACGAAAAGAAGTCGAATAAAAAAAAAAAg  <  1:2220298/128‑1 (MQ=255)
                                                                                                                             ||                                                                                                                         
AGAGCAGGAAGAGCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATAATTTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGAAAAAACAAAAAAAG  >  CP016816/19898‑20145

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: