BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP016816	31,676	C→T	A723V (GCA→GTA)	JCVSYN2_00105 →	ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP016816	31,676	0	C	T	99.8%	5012.7 / NA	1583	A723V (GCA→GTA)	JCVSYN2_00105	ABC transporter permease
Reads supporting (aligned to +/- strand): ref base C (1/1); major base T (809/767); minor base A (0/3); total (810/773)
Fisher's exact test for biased strand distribution p-value = 1.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1583 total aligned reads displayed.

TGTTGGAGTTATTAAGTCAATTCAAGCAAACTCTAAACAAATTGGTATTTTAAAAGCAAATGGAGCTTCAAGTGCAACTATTTCATGATCATATGTTTCATATGCTGTGATTTTAGTTTTTATAGCAATACCTTTAGGTTGAATGGCTGGAACAATGTTACAAGTTCCTTTTGTTGCTATTTTTAAAGATTATTTTAGTTTTAAAACAAATGTATTAATTTATGATTGATTAGCACCTTTAATATCTATT  >  CP016816/31551‑31800

 gttgGAGTTATTAAGTCAATTCAAGCAAACTCTAAACAAATTGGTATTTTAAAAGCAAATGGAGCTTCAAGTGCAACTATTTCATGATCATATGTTTCATATTCTTTTATTTTAGTTTTTATAGTAAt                                                                                                                           >  1:944671/1‑128 (MQ=255)

 gttgGAGTTATTAAGTCAATTCAAGCAAACTCTAAACAAATTGGTATTTTAAAAGCAAATGGAGCTTCAAGTGCAACTATTTCATGATCATATGTTTCATATGCTGTGATTTTAGTTTTTATAGTAAt                                                                                                                           >  2:431459/1‑128 (MQ=255)

  ttgGAGTTATTAAGTCAATTCAAGCAAACTCTAAACAAATTGGTATTTTAAAAGCAAATGGAGCTTCAAGTGCAACTTTTTCATGATCATATGTTTCATATGCTGTGATTTTAGTTTGTATAGTAATa                                                                                                                          >  2:1587811/1‑128 (MQ=255)

   tgGAGTTATTAAGTCAATTCAAGCAAACTCTAAACAAATTGGTATTTTAAAAGCAAATGGAGCTTCAAGTGCAACTATTTCATGATCATATGTTTCATATGCTGTGATTTTAGTTTTTATAGTAATAc                                                                                                                         >  1:627881/1‑128 (MQ=255)

    ggAGTTATTAAGTCAATTCAAGCAAACTCTAAACAAATTGGTATTTTAAAAGAAAATGGAGATTCAAGTGCAACTATTTCATGATCATATGTTTCATATGATTTGATTTTAGTTTTTATATTAATaac                                                                                                                        >  1:2041452/1‑126 (MQ=255)

      aggtattaAGTCAATTCAAGCAAACTCTAAACAAATTGGTATTATAAAAGCAAATGGAGCTTCAAGTGCAACTATTTCATGATCATATGTTTCATATGCTGTGATTTTAGTTTTTATAGTAATACCtt                                                                                                                      <  1:435299/125‑1 (MQ=255)

       gTTATTAAGTCAATTCAAGCAAACTCTAAACAAATTGGTATTTTAAAAGCAAATGGAGCTTCAAGTGCAACTATTTCATGATCATATGTTTCATATGCTGTGATTTTAGTTTTTATAGTAATACCttt                                                                                                                     >  1:3114628/1‑128 (MQ=255)

       gTTATTAAGTCAATTCAAGCAAACTCTAAACAAATTGGTATTTTAAAAGCAAATGGAGCTTCAAGTGCAACTATTTCATGATCATATGTTTCATATGCTGTGATTTTAGTTTTTATAGTAATACCttt                                                                                                                     >  1:3530369/1‑128 (MQ=255)

       gTTATTAAGTCAATTCAAGCAAACTCTAAACAAATTGGTATTTTAAAAGCAAATGGAGCTTCAAGTGCAACTATTTCATGATCATATGTTTCATATGCTGTGATTTTAGTTTTTATAGTAATACCttt                                                                                                                     <  2:2334940/128‑1 (MQ=255)

       gTTATTAAGTCAATTCAAGCAAACTCTAAACAAATTGGTATTTTAAAAGCAAATGGAGCTTCAAGTGCAACTATTTCATGATCATATGTTTCATATGCTGTGATTTTAGTTTTTATAGTAATACCtt                                                                                                                      <  2:335990/127‑1 (MQ=255)

          cttaAGTCAATTCAAGCAAACTCTAAACAAATTGGTATTTTAAAAGCAAATGGAGCTTCAAGTGCAACTATTTCATGATCATATGTTTCATATGCTGTGATTTTAGTTTTTATAGTAATACCTTTAgg                                                                                                                  <  1:319366/127‑1 (MQ=255)

               gTCAATTCAAGCAAACTCTAAACAAATTGGTATTTTAAAAGCAAATGGAGCTTCAAGTGCAACTATTTCATGATCATATGTTTCATATGCTGTGATTTTAGTTTTTATAGTAATACCTTTAGGTTGaa                                                                                                             >  2:3862994/1‑128 (MQ=255)

                tCAATTCAAGCAAACTCTAAACAAATTGGTATTTTAAAAGCAAATGGAGCTTCAAGTGCAACTATTTCATGATCATATGTTTCATATGCTGTGATTTTAGTTTTTATAGTAATACCTTTAGGTTGAAt                                                                                                            <  2:91881/128‑1 (MQ=255)

                 cAATTCAAGCAAACTCTAAACAAATTGGTATTTTAAAAGCAAATGGAGCTTCAAGTGCAACTATTTCATGATCATATGTTTCATATGCTGTGATTTTAGTTTTTATAGTAATACCTTTAGGTTGAATg                                                                                                           <  1:1491014/128‑1 (MQ=255)

                 cAATTCAAGCAAACTCTAAACAAATTGGTATTTTAAAAGCAAATGGAGCTGCAAGTGCAACTATTTCATGATCATATGTTTCATATGCTGTGATTTTAGTTTTTATAGTAATACCTTTAGGTTGAATg                                                                                                           <  2:2216995/128‑1 (MQ=255)

                   aTTCAAGCAAACTCTAAACAAATTGGTATTTTAAAAGCAAATGGAGCTTCAAGTGCAACTATTTCATGATCATATGTTTCATATGCTGTGATTTTAGTTTTTATAGTAATACCTTTATGTTGACTgga                                                                                                         >  2:502938/1‑127 (MQ=255)

                   aTTCAAGCAAACTCTAAACAAATTGGTATTTTAAAAGCAAATGGAGCTTCAAGTGCAACTATTTCATGATCATATGTTTCATATGCTGTGATTTTAGTTTTTATAGTAATACCTTTAGGTTGAATGGc                                                                                                         >  1:1428589/1‑128 (MQ=255)

                    ttCACGCAAACTCTAAACAAATTGGTATTTTAAAAGCAAATGGAGCTTCAAGTGCAACTATTTCATGATCATATGTTTCATATGCTGTGATTTTAGTTTTTATAGTAATACCTTTAGGTTGAATGGCt                                                                                                        <  1:2163290/128‑1 (MQ=255)

                    ataatgCATACTCTAAACAAATTGGTATTTTAAAAGCAATTGGATCTTCAAGTGCAACTATTTCATGATCATATGTTTCATATGCTGTGATTTTAGTTTTTATAGTAATACCTTTAGGTTGAATGGCt                                                                                                        <  1:1938358/123‑1 (MQ=255)

                     tCAAGCAAACTCTTAACTAATTGGTATTTTAAAAGCAAATGGAGCTTCTAGTGCAACTATTTCATGATCTTATGTTTCATATGCTGTGATTTTAGTTTTTATAGTAATACCTTTAGGTTGAATGGCTg                                                                                                       <  2:3452631/128‑1 (MQ=255)

                      cAAGCAAACTCTAAACAAATTGGTATTTTAAAAGCAAATGGAGCTTCAAGTGCAACTATTTCATGATCATATGTTTCATATGCTGTGATTTTAGTTTTTATAGTAATACCTTTAGGTTGAATGGCTgg                                                                                                      >  2:777002/1‑128 (MQ=255)

                      cAAGCAAACTCTAAACAAATTGGTATTTTAAAAGCAAATGGAGCTTCAAGTGCAACTATTTCATGATCATATGTTTCATATGCTGTGATTTTAGTTTTTATAGTAATACCTTTAGGTTGAATGGCTgg                                                                                                      >  1:3818173/1‑128 (MQ=255)

                            aaCTCTAAACAAATTGGTATTTTAAAAGCAAATGGAGCTTCAAGTGCAACTATTTCATGATCATATGTTTCATATGCTGTGATTTTAGTTTTTATAGTAATACCTTTAGGTTGAATGGCTGGAACAAt                                                                                                >  1:1787504/1‑128 (MQ=255)

                              ctctAAACAAATTGGTATTTTAAAAGCAAATGGAGCTTCAAGTGCAACTATTTCATGATCATATGTTTCATATGCTGTGATTTTAGTTTTTATAGTAATACCTTTAGGTTGAATGGCTGGAACAATGt                                                                                              <  2:3529009/128‑1 (MQ=255)

                               tctAAACAAATTGGTATTTTAAAAGCAAATGGAGCTTCAAGTGCAACTATTTCATGATCATATGTTTCATATGCTGTGATTTTAGTTTTTATAGTAATACCTTTAGGTTGAATGGCTGGAACAATGtt                                                                                             <  1:554679/128‑1 (MQ=255)

                                ctAAACAAATTGGTATTTTAAAAGCAAATGGAGCTTCAAGTGCAACTATTTCATGATCATATGTTTCATATGCTGTGATTTTAGTTTTTATAGTAATACCTTTAGGTTGAATGGCTGGAACAATGTTa                                                                                            >  1:100373/1‑128 (MQ=255)

                                 tAAACAAATTGGTATTTTAAAAGCAAATGGAGCTTCAAGTGCAACTATTTCATGCTCATATGGTTCAGATGCTGTGATTTTAGTTTTTATAGTAATACCTTTAGGTTGAATGGCTGGAACAATGTTAc                                                                                           >  2:2990375/1‑128 (MQ=255)

                                     cAAATTGGTATTTTAAAAGCAAATGGAGCTTCAAGTGCAACTATTTCATGATCATATGTTTCATATGCTGTGATTTTAGTTTTTATAGTAATACCTTTAGGTTGAATGGCTGGAACAATGTTACAAGt                                                                                       >  1:1734528/1‑128 (MQ=255)

                                     cAAATTGGTATTTTAAAAGCAAATGGAGCTTCAAGTGCAACTATTTCATGATCATATGTTTCATATGCTGTGATTTTAGTTTTTATAGTAATACCTTTAGGTTGAATGGCTGGAACAATGTTACAAGt                                                                                       >  1:3289226/1‑128 (MQ=255)

                                      aaaTTGGTATTTTAAAAGCAAATGGAGCTTCAAGTGCAACTATTTCATGATCATATGTTTCATATGCTGTGATTTTAGTTTTTATAGTAATACCTTTAGGTTGAATGGCTGGAACAATGTTACAAGtt                                                                                      <  2:3303750/128‑1 (MQ=255)

                                       aaTTGGTATTTTAAAAGCAAATGGAGCTTCAAGTGCAACTATTTCATGATCATATGTTTCATATGCTGTGATTTTAGTTTTTATAGTAATACCTTTAGGTTGAATGGCTGGAACAATGTTACAAGTTc                                                                                     >  2:958478/1‑128 (MQ=255)

                                             tATTTTAAAAGCAAATGGAGCTTCAAGTGCAACTATTTCATGATCATATGTTTCATATGCTGTGATTTTAGTTTTTATAGTAATACCTTTAGGTTGAATGGCTGGAACAATGTTACAAGTTCCTTttg                                                                               <  1:3766151/128‑1 (MQ=255)

                                              aTTTTAAAAGCAAATGGAGCTTCAAGTGCAACTATTTCATGATCATATGTTTCATATGCTGTGATTTTAGTTTTTATAGTAATACCTTTAGGTTGAATGGCTGGAACAATGTTACAAGTTCCTTttgt                                                                              >  2:132606/1‑128 (MQ=255)

                                               ttttAAAAGCAAATGGAGCTTCAAGTGCAACTATTTCATGATCATATGTTTCATATGCTGTGATTTTAGTTTTTATAGTAATACCTTTAGGTTGAATGGCTGGAACAATGtt                                                                                             <  2:2698086/112‑1 (MQ=255)

                                                tttAAAAGCAAATGGAGCTTCAAGTGCAACTATTTCATGATCATATGTTTCATATGCTGTGATTTTAGTTTTTATAGTAATACCTTTAGGTTGAATGGCTGGAACAATGTTACAAGTTCCTTttgttg                                                                            >  1:3015574/1‑128 (MQ=255)

                                                tttAAAAGCAAATGGAGCTTCAAGTGCAACTATTTCATGATCATATGTTTCATATGCTGTGATTTTAGTTTTTATAGTAATACCTTTAGGTTGAATGGCTGGAACAATGTTACAAGTTCCTTttgttg                                                                            >  2:3794452/1‑128 (MQ=255)

                                                 ttAAAAGCAAATGGAGCTTCAAGTGCAACTATTTCATGATCATATGTTTCATATGCTGTGATTTTAGTTTTTATAGTAATACCTTTAGGTTGAATGGCTGGAACAATGTTACAAGTTCCTTTTGTTGc                                                                           >  1:492556/1‑128 (MQ=255)

                                                     aaGCAAATGGAGCTTCAAGTGCAACTATTTCATGATCATATGTTTCATATGCTGTGATTTTAGTTTTTATAGTAATACCTTTAGGTTGAATGGCTGGAACAATGTTACAAGTTCCTTTTGTTGCTAtt                                                                       <  1:3353412/128‑1 (MQ=255)

                                                       gCAAATGGAGCTTCAAGTGCAACTATTTCATGATCATATGTTTCATATGCTGTGATTTTAGTTTTTATAGTAATACCTTTAGGTTGAATGGCTGGAACAATGTTACAAGTTCCTTTTGTTGCTAtttt                                                                     >  2:3222555/1‑128 (MQ=255)

                                                         aaaTGGAGCTTCAAGTGCAACTATTTCATGATCATATGTTTCATATGCTGTGATTTTAGTTTTTATAGTAATACCTTTAGGTTGAATGGCTGGAACAATGTTACAAGTTCCTTTTGTTGCTATTTTTa                                                                   <  1:1874088/128‑1 (MQ=255)

                                                           aTGGAGCTTCAAGTGCAACTATTTCATGATCATATGTTTCATATGCTGTGATTTTAGTTTTTATAGTAATACCTTTAGGTTGAATGGCTGGAACAATGTTACAAGTTCCTTTTGTTGCTATTTTTaaa                                                                 >  1:252998/1‑128 (MQ=255)

                                                           aTGGAGCTTCAAGTGCAACTATTTCATGATCATATGTTTCATATGCTGTGATTTTAGTTTTTATAGTAATACCTTTAGGTTGAATGGCTGGAACAATGTTACAAGTTCCTTTTGTTGCTATTTTTaaa                                                                 >  1:269086/1‑128 (MQ=255)

                                                           aTGGAGCTTCAAGTGCAACTATTTCATGAGCATATGATTCATATGCTGTGATTTTAGTTTTTATAGTAATACCTTTAGGTTGAATGGCTGGAACAATGTTACAAGTTCCTTTTGTTGCTATTTTTaaa                                                                 <  2:3604057/128‑1 (MQ=255)

                                                            tgcaGCTTCAAGTGCAACTATTTCATGATCATATGTTTCATATGCTGTGATTTTAGTTTTTATAGTAATACCTTTAGGTTGAATGGCTGGAACAATGTTACAAGTTCCTTTTGTTGCTATTTTTAAAg                                                                <  1:996337/125‑1 (MQ=255)

                                                            tGGTGCTTCAAGTGCAACTATTTCATGATCATATGATTCTTATGCTGTGATTTTAGTTTTTATTGTAATACCTTTAGGTTGAATGGCTGGTACAATGTTACAAGTTCCTTTTGTTGCTATTTTTAAAg                                                                <  1:1660498/128‑1 (MQ=255)

                                                             gttcttagatttGCAAATATTACATGATGATATGTTTCATATGATGTGATTTTAGTTTTTATAGTAATACCTTTAGGTTGAATGGCTGGAACAATGTTACAAGTTCCTTTTGTTGCTATTTTTAAAGa                                                               <  2:1076504/117‑1 (MQ=255)

                                                             ggAGCTTCAAGTGCAACTATTTCATGATCATATGTTTCATATGCTGTGATTTTAGTTTTTATAGTAATACCTTTAGGTTGAATGGCTGGAACAATGTTACAAGTTCCTTTTGTTGCTATTTTTATAGa                                                               >  1:1273421/1‑128 (MQ=255)

                                                             ggAGCTTCAAGTGCAACTATTTCATGATCATATGTTTCATATGCTGTGATTTTAGTTTTTATAGTAATACCTTTAGGTTGAATGGCTGGAACAATGTTACAAGTTCCTTTTGTTGCTATTTTTAAAGa                                                               >  1:801957/1‑128 (MQ=255)

                                                               aGCTTCAAGTGCAACTATTTCATGATCATATGTTTCATATGCTGTGATGTTAGTTTTTATAGTAATACCTTTAGGTTGAATGGCTGGAACTATGTTACAAGTTCCTTGTGTTGCTATTTTTAAAGatt                                                             >  1:2293313/1‑128 (MQ=255)

                                                                gCTTCAAGTGCAACTATTTCATGATCATATGTTTCATATGCTGTGATTTTAGTTTTTATAGTAATACCTTTAGGTTGAATGGCTGGAACAATGTTACAAGTTCCTTTTGTTGCTATTTTTAAAGatta                                                            <  1:2460087/128‑1 (MQ=255)

                                                                  ttCAAGTGCAACTATTTCATGATCATATGTTTCATATGCTGTGATTTTAGTTTTTATAGTAATACCTTTAGGTTGAATGGCTGGAACAATGTTACAAGTTCCTTTTGTTGCTATTTTTAAAGattatt                                                          >  2:1167562/1‑128 (MQ=255)

                                                                  ttCAAGTGCAACTATTTCATGATCATATGTTTCATATGCTGTGATTTTAGTTTTTATAGTAATACCTTTAGGTTGAATGGCTGGAACAATGTTACAAGTTCCTTTTGTTGCTATTTTTAAAGattatt                                                          >  2:3698669/1‑128 (MQ=255)

                                                                    cAAGTGCAACTATTTCATGATCATATGTTTCATATGCTGTGATTTTAGTTTTTATAGTAATACCTTTAGGTTGAATGGCTGGAACAATGTTACAAGTTCCTTTTGTTGCTATTTTTAAAGATTAtttt                                                        <  1:2222875/128‑1 (MQ=255)

                                                                     aaGTGCAACTATTTCATGATCATATGTTTCATATGCTGTGATTTTAGTTTTTATAGTAATACCTTTAGGTTGAATGGCTGGAACAATGTTACAAGTTCCTTttgtt                                                                             >  1:3866210/1‑106 (MQ=255)

                                                                     aaGTGCAACTATTTCATGATCATATGTTTCATATGCTGTGATTTTAGTTTTTATAGTAATACCTTTAGGTTGAATGGCTGGAACAATGTTACAAGTTCCTTTTGTTGCTATTTTTAATGATTATTTTa                                                       >  1:2841181/1‑128 (MQ=255)

                                                                     aaGTGCAACTATTTCATGATCATATGTTTCATATGCTGTGATTTTAGTTTTTATAGTAATACCTTTAGGTTGAATGGCTGGAACAATGTTACAAGTTCCTTTTGTTGCTATTTTTAAAGATTATTTTa                                                       >  2:1526889/1‑128 (MQ=255)

                                                                     aaGGGCAACTATTTCATGATCATATGTTTCATATGCTGTGATTTTAGTTTTTATAGTAATACCTTTAGGTTGAATGGCTGGAACAATGTTACAAGTTCCTTTTGTTGCTATTTTTAAAGATTATTTTa                                                       <  2:1787504/128‑1 (MQ=255)

                                                                       gTGCTACTATTTCATGATCATATGTTTCATATGCTGTGATTTTAGTTTTTATAGTAATACCTTTAGGTTGAATGGCTGGAACAATGTTACAAGTTCCTTTTGTTGCTATTTTTAAAGATTATTTTAGt                                                     <  1:701004/128‑1 (MQ=255)

                                                                        tGCAACTATTTCATGATCATATGTTTCATATGCTGTGATTTTAGTTTTTATAGTAATACCTTTAGGTTGAATGGCTGGAACAATGTTACAAGTTCCTTTTGTTGCTATTTTTAAAGATTATTTTAGtt                                                    >  1:3935966/1‑128 (MQ=255)

                                                                        tGCAACTATTTCATGATCATATGTTTCATATGCTGTGATTTTAGTTTTTATAGTAATACCTTTAGGTTGAATGGCTGGAACAATGTTACAAGTTCCTTTTGTAGCTATTTTTAAAGATTATTTTAGtt                                                    <  1:1581157/128‑1 (MQ=255)

                                                                          ccactcattCATGATCATATGTTTCATATGCTGTGATTTTAGTTTTTATAGTAATACCTTTAGGGTGAATGGCTGGAACAATGTTACAAGGTCCTTTTGTTGCTATTTTTAAAGATTATTTTAGtttt                                                  <  2:3751251/121‑1 (MQ=255)

                                                                             cTATTTCATGATCATATGTTTCATATGCTGTGATTTTAGTTTTTATAGTAATACCTTTAGGTTGAATGGCTGGAACAATGTTACAAGTTCCTTTTGTTGCTATTTTTAAAGATTATTTTAGTTTTaaa                                               >  2:548885/1‑128 (MQ=255)

                                                                                 ttCATGATCATATGTTTCATATGCTGTGATTTTAGTTTTTATAGTAATACCTTTAGGTTGAATGGCTGGAACAATGTTACAAGTTCCTTTTGTTGCTATTTTTAAAGATTATTTTAGTTTTAAAACaa                                           <  2:2428533/128‑1 (MQ=255)

                                                                                 ttCATGATCATATGTTTCATATGCTGTGATTTTAGTTTTTATAGTAATACCTTTAGGTTGAATGGCTGGAACAATGTTACAAGTTCCTTTTGTTGCTATTTTTAAAGATTATTTTAGTTTTAAAACaa                                           <  2:3079137/128‑1 (MQ=255)

                                                                                    aTGATCATATGTTTCATATGCTGTGATTTTAGTTTTTATAGTAATACCTTTAGGTTGAATGGCTGGAACAATGTTACAAGTTCCTTTTGTTGCTATTTTTAAAGATTATTTTAGTTTTAAATCAAATg                                        >  2:629507/1‑128 (MQ=255)

                                                                                       aTCATATGTTTCATATGCTGTGATTTTAGTTTTTATAGTAATACCTTTAGGTTGAATGGCTGGAACAATGTTACAAGTTCCTTTTGTTGCTATTTTTATAGATTATTTTAGTTTTAAAACAATTGTAt                                     >  1:747303/1‑128 (MQ=255)

                                                                                       aTCATATGTTTCATATGCTGTGATTTTAGTTTTTATAGTAATACCTTTAGGTTGAATGGCTGGAACAATGTTACAAGTTCCTTTTGTTGCTATTTTTAAAGATTATTTTAGTTTTAAAACAAATGTAt                                     >  2:853355/1‑128 (MQ=255)

                                                                                         cATATGTTTCATATGCTGTGATTTTAGTTTTTATAGTAATACCTTTAGGTTGAATGGCTGGAACAATGTTACAAGTTCCTTTTGTTGCTATTTTTAAAGattat                                                           >  2:277489/1‑104 (MQ=255)

                                                                                         cATATGTTTCATATGCTGTGATTTTAGTTTTTATAGTAATACCTTTAGGTTGAATGGCTGGAACAATGTTACAAGTTCCTTTTGTTGCTATTTTTAAAGATTATTTTAGTTTTAAAACAAATGTATTa                                   <  2:1416581/128‑1 (MQ=255)

                                                                                           tatGTTTCATATGCTGTGATTTTAGTTTTTATAGTAATACCTTTAGGTTGAATGGCTGGAACAATGTTACAAGTTCCTTTTGTTGCTATTTTTAAAGATTATTTTAGTTTTAAAACAAATGTATTAAt                                 <  2:2046712/128‑1 (MQ=255)

                                                                                           tatGTTTCATATGCTGTGATTTTAGTTTTTATAGTAATACCTTTAGGTTGAATGGCTGGAACAATGTTACAAGTTCCTTTTGTTGCTATTTTTAAAGATTATTTTAGTTTTAAAACAAATGTATTAAt                                 <  1:3475844/128‑1 (MQ=255)

                                                                                           tatGTTTCATATGCTGTGATTTTAGTTTTTATAGTAATACCTTTAGGTTGAATGGCTGGAACAATGTTACAAGTTCCTTTTGTTGCTATTTTTAAAGATTATTTTAGTTTTAAAACAAATGTATTAAt                                 <  2:1258302/128‑1 (MQ=255)

                                                                                              gTTTCTTATTCTGTTATTTTAGTTTTTATAGTAATACCTTTAGGTTGAATGGCTGGAACAATGTTACAAGTTCCTTTTGTTGCTATTTTTAAAGATTATTTTAGTTTTAAAACAAATGTATTAATTTa                              <  2:3150045/128‑1 (MQ=255)

                                                                                              gTTTCATATGCTGTGATTTTAGTTTTTATAGTAATACCTTTAGGTTGAATGGCTGGAACAATGTTACAAGTTCCTTTTGTTGCTATTTTTAAAGATTATTTTAGTTTTAAAACAAATGTATTAATTTa                              <  1:1212200/128‑1 (MQ=255)

                                                                                                ttCATATGCTGTGATTTTAGTTTTTATAGTAATACCTTTAGGTTGAATGGCTGGAACAATGTTACAAGTTCCTTTTGTTTCTATTTTTAAAGATTATTGTAGTTTTAAAACAAATGTATTAATTTAtg                            >  1:2089812/1‑128 (MQ=255)

                                                                                                ttCATATGCTGTGATTTTAGTTTTTATAGTAATACCTTTAGGTTGAATGGCTGGAACAATGTTACAAGTTCCTTTTGTTGCTATTTTTAAAGATTATTTTAGTTTTAAAACAAATGTATTAATTTAtg                            >  2:3947303/1‑128 (MQ=255)

                                                                                                 tCATATGCTGTGATTTTAGTTTTTATAGTAATACCTTTAGGTTGAATGGCTGGAACAATGTTACAAGTTCCTTTTGTTGCTATTTTTAAAGATTATTTTAGTTTTAAAACAAATGTATTAATTTAtga                           >  2:252709/1‑128 (MQ=255)

                                                                                                 tCATATGCTGTGATTTTAGTTTTTATAGTAATACCTTTAGGTTGAATGGCTGGAACAATGTTACAAGTTCCTTTTGTTGCTATTTTTAAAGATTATTTTAGTTTTAAAACAAACGTATTAATTTAtga                           <  2:1870819/128‑1 (MQ=255)

                                                                                                 tCATATGCTGTGATTTTAGTTTTTATAGTAATACCTTTAGGTTGAATGGCTGGAACAATGTTACAAGTTCCTTATGTTGCTATATTTAAAGATTATTTTAGTTTTAAAACAAATGTATTAATTTAtga                           >  1:2608932/1‑128 (MQ=255)

                                                                                                  cATATGCTGTGATTTTAGTTTTTATAGTAATACCTTTAGGTTGAATGGCTGGAACAATGTTACAAGTTCCTTTTGTTGCTATTTTTAAAGATTATTTTAGTTTTAAAACAAATGTATTAATTTGtgat                          >  2:2823480/1‑128 (MQ=255)

                                                                                                      tGCTGTGATTTTAGTTTTTATAGTAATACCTTTAGGTTGAATGGCTGGAACAATGTTACAAGTTCCTTTTGTTGCTATTTTTAAAGATTATTTTAGTTTTAAAACAAATGTATTAATTTAtgattgat                      >  1:1354979/1‑128 (MQ=255)

                                                                                                        cTGTGATTTTAGTTTTTATAGTAATACCTTTAGGTTGAATGGCTGGAACAATGTTACAAGTTCCTTTTGTTGCTATTTTTAAAGATTATTTTAGTTTTAAAACAAATGTATTAATTTATGATTGATTa                    >  1:2735358/1‑128 (MQ=255)

                                                                                                         tgtgAGTTTAGTTTTTATAGAAATACCTTTAGGTTGAATGGCTGGATCTATGTTATATGTTCCTTTTGTTGCTATTTTTAAAGATTATTTTAGTTTTAAAACAAATGTATTAATTTATGATTGATTAg                   <  2:168498/128‑1 (MQ=255)

                                                                                                           tgATTTTAGTTTTTATAGTACTACCTTTAGGTTGAATGGCTGGAACAATGTTACAAGTTCCTTTTGTTGCTATTTTTAAAGATTATTTTAGTTTTAAAACAAATGTATTAATTTATGATTGATTAGCa                 <  1:2382708/128‑1 (MQ=255)

                                                                                                           tgATTTTAGTTTTTATAGTAATACCTTTAGGTTGAATGGCTGGAACAATGTTACAAGTTCCTTTTGTTGCTATTTTTAAAGATTATTTTAGTTTTAAAACAAATGTATTAATTTATGATTGATTAGCa                 <  1:867232/128‑1 (MQ=255)

                                                                                                             aTTTTAGTTTTTATAGTAATACCTTTAGGTTGAATGGCTGGAACAATGTTACAAGTTCCTTTTGTTTCTATTTTTAAAGATTATTTTAGTTTTAAAACAAATGTAtt                                    <  2:2907038/107‑1 (MQ=255)

                                                                                                             aTTTTAGTTTTTATAGTAATACCTTTAGGTTGAATGGCTGGAACAATGTTACAAGTTCCTTTTGTTGCTATTTTTAAAGATTATTTTAGTTTTAAAACAAATGTAtt                                    >  1:2907038/1‑107 (MQ=255)

                                                                                                              ttttAGTTTTTATAGTAATACCTTTAGGTTGAATGGCTGGAACAATGTTACAAGTTCCTTTTGTTGCTATTTTTAAAGATTATTTTAGTTTTAAAACAAATGTATTAATTTATGATTGATTAGCACCt              >  1:1134288/1‑128 (MQ=255)

                                                                                                                ttAGTTTTTATAGTAATACCTTTAGGTTGAATGGCTGGAACAATGTTACAAGTTCCTTTTGTTGCTATTTTTAAAGATTATTTTAGTTTTAAAACAAATGTATTAATTTATGATTGATTAGCACCttt            >  2:1454106/1‑128 (MQ=255)

                                                                                                                 tAGTTTTTATAGTAATACCTTTAGGTTGAATGGCTGGAACAATGTTACAAGTTCCTTTTGTTGCTATTTTTAAAGATTATTTTAGTTTTAAAACAAATGTATTAATTTATGATTGATTAGCACCTTTa           >  2:2283578/1‑128 (MQ=255)

                                                                                                                  aGTTTTTATAGTAATACCTTTAGGTTGAATGGCTGGAACAATGTTACAAGTTCCTTTTGTTGCTATTTTTAAAGATTATTTTAGTTTTAAAACAAATGTATTAATTTATGATTGATTAGCACCTTTaa          >  2:1955456/1‑128 (MQ=255)

                                                                                                                     ttttATAGTAATACCTTTAGGTTGAATGGCTGGAACAATGTTACAAGTTCCTTTTGTTGCTATTTTTAAAGATTATTTTAGTTTTAAAACAAATGTATTAATTTATGATTGATTAGCACCTTTAatat       >  1:340055/1‑128 (MQ=255)

                                                                                                                       ttATAGTAATACCTTTAGGTTGAATGGCTGGAACAATGTTACAAGTTCCTTTTGTTGCTATTTTTAAAGATTATTTTAGTTTTAAAACAAATGTATTAATTTATGATTGATTAGCACCTTTAATATCt     >  1:3609624/1‑128 (MQ=255)

                                                                                                                       ttATAGTAATACCTTTAGGTTGAATGGCTGGAACAATGTTACAAGTTCCTTTTGTTGCTATTTTTAAAGATTATTTTAGTTTTAAAACAAATGTATTAATTTATGATTGATTAGACCATTTAatatat     >  2:210079/1‑126 (MQ=255)

                                                                                                                       ttATAGTAATACCTTTAGGTTGAATGGCTGGAACAATGTTACAAGTTCCTTTTGTTGCTATTTTTAAAGATTATTTTAGTTTTAAAACAAATGTATTAATTTATGATTGATAAGCACCTTTAATATCt     >  2:2591682/1‑128 (MQ=255)

                                                                                                                        tctaGAAATCCCTTTAGGTTGAATGGATGGAACAATGTTACAAGTTCCTTTTGTTGCTATTTTTAAAGATTATTTTAGTTTTAAAACAAATGTATTAATTTATGATTGATTAGCACCTTTAATATCta    <  1:2793094/126‑1 (MQ=255)

                                                                                                                         ataGTAATACCTTTATGTTGAATGGCTGGAACAATGTTACAAGTTCCTTTTGTTGCTATTTTTAAAGATTATTTTAATTTTAAAACAAATGTATTAATTTATGATTGATTAGCACCTTTAATATCtat   <  1:3698669/128‑1 (MQ=255)

                                                                                                                         ataGTAATACCTTTAGGTTGAATGGCTGGAACAATGTTACAAGTTCCTTTTGTTGCTATTTTTAAAGATTATTTTAGTTTTAAAACAAATGTATTAATTTATGATTGATTAGCACCTTTAATATCtat   >  2:703939/1‑128 (MQ=255)

                                                                                                                          taGTAATACCTTTAGGTTGAATGGCTGGAACAATGTTACAAGTTCCTTTTGTTGCTATTTTTAAAGATTATTTTAGTTTTAAAACAAATGTATTAATTTATGATTGATTAGCACCTTTAATATCtatt  <  2:217706/128‑1 (MQ=255)

                                                                                                                          taGTAATACCTTTAGGTTGAATGGCTGGAACAATGTTACAAGTTCCTTTTGTTGCTATTTTTAAAGATTATTTTAGTTTTAAAACAAATGTATTAATTTATGATTGATTAGCACCTTTAATATCtatt  >  1:1057628/1‑128 (MQ=255)

TGTTGGAGTTATTAAGTCAATTCAAGCAAACTCTAAACAAATTGGTATTTTAAAAGCAAATGGAGCTTCAAGTGCAACTATTTCATGATCATATGTTTCATATGCTGTGATTTTAGTTTTTATAGCAATACCTTTAGGTTGAATGGCTGGAACAATGTTACAAGTTCCTTTTGTTGCTATTTTTAAAGATTATTTTAGTTTTAAAACAAATGTATTAATTTATGATTGATTAGCACCTTTAATATCTATT  >  CP016816/31551‑31800

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`