Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 484,795 A→T intergenic (‑104/+67) JCVSYN2_02220 ← / ← JCVSYN2_02225 hypothetical protein/50S ribosomal protein L7/L12

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816484,7950AT99.3% 5230.7 / ‑0.1 1746intergenic (‑104/+67)JCVSYN2_02220/JCVSYN2_02225hypothetical protein/50S ribosomal protein L7/L12
Reads supporting (aligned to +/- strand):  ref base A (6/7);  new base T (843/890);  total (849/897)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1808 total aligned reads displayed.

TAAAAAATAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTTAAAAAAAAAACAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATTTCTTCAGC  >  CP016816/484671‑484918
                                                                                                                             |                                                                                                                            
 aaaaaaTAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAatataaaa                                                                                                                          >  2:2198214/1‑128 (MQ=255)
 aaaaaaTAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAatataaaa                                                                                                                          <  1:3696035/128‑1 (MQ=255)
  aaaaaTAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAatataaaaa                                                                                                                         >  1:2504558/1‑128 (MQ=255)
    aaaTAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAatataaaaagc                                                                                                                       >  2:3160265/1‑126 (MQ=255)
        aGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTATCAACTAAACTACACTCGATTATTTAATTATATTTTTATTTAATATAAAAAGAAAtt                                                                                                                   >  1:2569512/1‑128 (MQ=255)
        aGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAAtt                                                                                                                   >  2:3369361/1‑128 (MQ=255)
           cAAAACTCATAGATTTAATCCTTTTATAGTTGACCATAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTaaa                                                                                                                <  2:3295464/128‑1 (MQ=255)
            aaaaCTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAATCTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAac                                                                                                               >  2:1923442/1‑128 (MQ=255)
             aaaCTCATAGATTTAATCCTTTTATACTTGACCAAAAAAAAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCCAAAAATTAATTATATTTATTATTAAAATAAAAAGCAATTAAcca                                                                                                              >  1:980816/1‑128 (MQ=255)
              aaCTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAAATAAacac                                                                                                             >  2:1044416/1‑128 (MQ=255)
               aCTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAacaaa                                                                                                            >  2:447139/1‑126 (MQ=255)
               aCTCATAGATTTAATCATTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAacaca                                                                                                            <  2:541085/128‑1 (MQ=255)
                cTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAacacac                                                                                                           <  2:1611804/128‑1 (MQ=255)
                cTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAAATAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAacacac                                                                                                           >  2:1524624/1‑128 (MQ=255)
                 tCATAGATTTAATCCTTTTATTGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAAcacaca                                                                                                          >  2:2897007/1‑128 (MQ=255)
                 tCATAGATTTAATACTTTTATAGATGACCAAAAAATAAGATAGAGCATAGATGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAAcacaca                                                                                                          <  2:3418732/128‑1 (MQ=255)
                      gATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTATTAATATAAAAAGCAATTAAACACACAAAAAc                                                                                                     >  2:3622090/1‑128 (MQ=255)
                      gATTTAATACTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAAc                                                                                                     <  2:2046710/128‑1 (MQ=255)
                       aTTTAATCATTTTATAGTTGACCAAAAAATAAGATAGAGCAAAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATATAATTATATTTTTTATTTAATATAAAAAGCAATTAAAAACACAAAAAc                                                                                                     >  1:1903120/1‑128 (MQ=255)
                        tttAATCCTTTTATAGTTGCCCAAAAAATAAGATATAGCATAGCAGAAGCTCATAACAACTAAACTACACTCTAATATTTAATAATATATTTTTTTAATATAAAACTCAATAAATCACACAAAAACta                                                                                                   >  2:232391/1‑127 (MQ=255)
                        tttAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTc                                                                                                   <  1:3219235/128‑1 (MQ=255)
                        tttAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTc                                                                                                   >  2:1850427/1‑128 (MQ=255)
                        tttAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAAATATATTTATTATTAATATAAAAAGCAATTAAACACACAAAAACTc                                                                                                   >  1:1358325/1‑128 (MQ=255)
                           aaTCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAATAAGCAATTAAACACACAAAAACTCAcc                                                                                                >  2:3941546/1‑128 (MQ=255)
                            aTCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAccc                                                                                               >  2:1359503/1‑128 (MQ=255)
                            aTCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAccc                                                                                               >  2:3779244/1‑128 (MQ=255)
                            aTCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAcca                                                                                               <  1:1696081/128‑2 (MQ=255)
                             tCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAccat                                                                                              <  1:2190474/128‑3 (MQ=255)
                             tCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCa                                                                                              >  1:182317/1‑128 (MQ=255)
                               cTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCAtt                                                                                            >  2:112364/1‑128 (MQ=255)
                                ttttATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAccataat                                                                                           <  1:3144478/128‑6 (MQ=255)
                                 tttATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAccataatg                                                                                          <  1:1281136/128‑7 (MQ=255)
                                 tttATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTAt                                                                                         >  1:690838/1‑128 (MQ=255)
                                 tttATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTAt                                                                                         <  1:244373/128‑1 (MQ=255)
                                 tttATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTAt                                                                                         >  1:2925759/1‑128 (MQ=255)
                                  ttATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATg                                                                                        >  1:2131152/1‑128 (MQ=255)
                                  ttATAGTCGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAccataatgg                                                                                         <  2:144890/128‑8 (MQ=255)
                                   tataGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATgg                                                                                       >  1:3407655/1‑128 (MQ=255)
                                    ataGTTGACCAAAAAATAAGATAGATCATAGCAGAATCTCTTAACAACTAAACTACACTCGATTATTTTATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAACCTCACCCATTATGGt                                                                                      <  1:3965943/128‑1 (MQ=255)
                                    ataGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATTGt                                                                                      >  1:3466334/1‑128 (MQ=255)
                                       gTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAg                                                                                   >  2:943366/1‑128 (MQ=255)
                                       gTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAg                                                                                   <  1:1757702/128‑1 (MQ=255)
                                       gTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCAATTATGGTGAg                                                                                   >  1:3858515/1‑128 (MQ=255)
                                          gACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCTATTAAACACACAAAAACTCACCCATTATGGGGAGttt                                                                                >  2:1762944/1‑128 (MQ=255)
                                          gACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGttt                                                                                >  2:3842197/1‑128 (MQ=255)
                                           aCCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATTGTGAGTTTa                                                                               >  1:83127/1‑128 (MQ=255)
                                            ccAAAAAATAAGATAGAGCATAGCAGAATCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTaa                                                                              <  1:753989/128‑1 (MQ=255)
                                            ccAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACATCTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTaa                                                                              <  1:1064912/128‑1 (MQ=255)
                                              aaaaaaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTaaaa                                                                            <  1:2264712/128‑1 (MQ=255)
                                              aaaaaaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTaaaa                                                                            >  2:678054/1‑128 (MQ=255)
                                               aaaaaTTAGATAGAGCATAGCAGAAGCTCTTAACAAATAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTaaaaa                                                                           <  2:1184848/128‑1 (MQ=255)
                                               aaaaaTAAGATAGAGCATATCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTaaaaa                                                                           <  1:2843708/128‑1 (MQ=255)
                                                     aaGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATAATTc                                                                     >  1:100120/1‑128 (MQ=255)
                                                      aGATAGAGCTTAGCAGAAGCTCTTAACAACTAAACTACTCTCGATTATTTAATTAAATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGTTGAGTTTAATAATTATTCa                                                                    >  2:199617/1‑128 (MQ=255)
                                                      aGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCa                                                                    >  1:3563120/1‑128 (MQ=255)
                                                      aGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCa                                                                    <  1:1985761/128‑1 (MQ=255)
                                                           gagCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTTTTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAAATATTCAAAAAt                                                               >  2:2770314/1‑128 (MQ=255)
                                                           gagCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAAt                                                               <  2:3483139/128‑1 (MQ=255)
                                                           gagCATAGCAGAAGCTCTTAAAAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAAt                                                               <  2:301211/128‑1 (MQ=255)
                                                           gagCATAGAATAAGCTCTTAACATCTAAACTACAATCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAAt                                                               <  1:1448000/128‑1 (MQ=255)
                                                            agCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCAATATGGTGAGTTTAAAAATTATTCAAAAAtt                                                              >  1:463525/1‑128 (MQ=255)
                                                             gCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAAtgt                                                             >  1:2996363/1‑126 (MQ=255)
                                                             gCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTAAATATAAAAAGCAATAAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATAATTCAAAAAttt                                                             >  1:621352/1‑128 (MQ=255)
                                                              cATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTg                                                            >  2:3080712/1‑128 (MQ=255)
                                                                tAGCATAAGCTCTTAACAACTAAACTACAATCGATTATTTAATTATATTATTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACAAATTATGTTAATATTAAAAATAATTCAAAAAttttaa                                                          >  1:2644146/1‑125 (MQ=255)
                                                                 atcAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGatt                                                         <  1:3513341/126‑1 (MQ=255)
                                                                 aGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGa                                                                                    <  2:2286706/101‑1 (MQ=255)
                                                                  gCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGatta                                                        <  1:904806/128‑1 (MQ=255)
                                                                    aGAAGCTCTTAACTACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGattatt                                                      <  2:369698/128‑1 (MQ=255)
                                                                     gAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCTCCCATTAAGGATAGTTTAAAAATTATTCAAAAAATAGATTAttt                                                     >  1:1544455/1‑128 (MQ=255)
                                                                     gAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGatttaaa                                                     <  2:60147/128‑5 (MQ=255)
                                                                      aaGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTAtttt                                                    >  2:731160/1‑128 (MQ=255)
                                                                        gCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTaa                                                  <  2:782753/128‑1 (MQ=255)
                                                                         ctctTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTaaa                                                 <  1:533036/128‑1 (MQ=255)
                                                                          tctTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAAt                                                >  2:2705452/1‑128 (MQ=255)
                                                                            ttAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCa                                              <  1:3362485/128‑1 (MQ=255)
                                                                            ttAACAACTAAACTACACTAGATTATTTAATTATATTTTTTATTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTGATGGGAGTTGAAAAATTAATCATAACTTTGATTATGTTACATc                                               >  2:2483316/1‑128 (MQ=255)
                                                                                 aactaaactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGat                                                          <  2:2561944/111‑1 (MQ=255)
                                                                                 aactaaactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGa                                         >  1:3275870/1‑128 (MQ=255)
                                                                                 aactaaactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGa                                         <  1:2318613/128‑1 (MQ=255)
                                                                                 aactaaactaCACTCGAATATTTAATTATATTTTTTTTTAATAAAAAAACCAATTAAACACACAAAAACTCACCCATAATGTTGAGTTTAAAAATAATTCAAAAATTTCATAATATTAAATAAATTGa                                         >  2:3541281/1‑128 (MQ=255)
                                                                                     aaactaCACTCGATTAATTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCt                                     <  1:2705777/128‑1 (MQ=255)
                                                                                       actaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATCCAAAAATTTGATTATTTTAAATCAGTTGAAGCtgg                                   <  2:1269351/128‑3 (MQ=255)
                                                                                          acacTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTTATTATTTTAAATCAATTTAAGCTCCAga                                >  2:2134029/1‑127 (MQ=255)
                                                                                          acacTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGc                                >  2:2408706/1‑128 (MQ=255)
                                                                                          acacTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGc                                <  1:1123147/128‑1 (MQ=255)
                                                                                          acacTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACAAACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGc                                <  1:3632175/128‑1 (MQ=255)
                                                                                               ctattattTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCAATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCaa                           <  2:2645747/126‑1 (MQ=255)
                                                                                               cGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGATCCAGCTTCaa                           >  2:1673547/1‑128 (MQ=255)
                                                                                                 attattTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACt                         <  2:850339/128‑1 (MQ=255)
                                                                                                  ttattTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTa                        >  2:3241004/1‑128 (MQ=255)
                                                                                                    ctttAATTATATTTTTTTTTATTATATTAAGCATTTAAAGACACAAAAAGTCACCCATTATGGTGAGTTTAAAATTTATTCTATAATTTGTTTTTTTTATATCAATTGAAGCTCCAGCTTCAACTAAt                      <  1:206483/127‑1 (MQ=255)
                                                                                                    attTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAAt                      <  1:2388113/128‑1 (MQ=255)
                                                                                                        aaTTATATTTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTa                   <  1:2785048/128‑1 (MQ=255)
                                                                                                        aaTTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAg                  <  1:1633171/128‑1 (MQ=255)
                                                                                                         ataatatTTTTTTTTAATATAAAAAGAAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGc                 <  2:2833130/125‑1 (MQ=255)
                                                                                                            atatTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCttt              >  1:1206238/1‑128 (MQ=255)
                                                                                                            atatTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCttt              >  2:2970854/1‑128 (MQ=255)
                                                                                                                 tttttttaATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCAttt         <  1:3781648/128‑1 (MQ=255)
                                                                                                                   tttttaATATAAAAATCAATTAAACAAACAAAAACTCAACCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTACAGCTTCAACTAATTTAGCTTTCATttct       <  1:3044396/128‑1 (MQ=255)
                                                                                                                             |                                                                                                                            
TAAAAAATAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTTAAAAAAAAAACAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATTTCTTCAGC  >  CP016816/484671‑484918

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: