Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 408,277 A→T 6.8% E34V (GAA→GTA)  yaiE → UPF0345 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913408,2770AT 6.8% 180.0 / 3.2 59E34V (GAA→GTA) yaiEUPF0345 family protein
Reads supporting (aligned to +/- strand):  ref base A (23/32);  new base T (2/2);  total (25/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.82e-01

GTCATCATGCTGGATTGTTTAGGATTTCACCAACGTCATCGCGATTTACTGCAAAAACAGCTCGATGTTCCTGTCTTGCTGTCTAACGTATTGATTGCACGGCTGGCTGCGGAATTACTGGTGTAATTTTGCGTGACAGCCAGCGCCTCTGGCCCCTATAGTGAAGTAGATGTTCAACTACCAAACAGGGCCAGTTTATGCTTCAAAGTAATGAGTACTTTTCCGGCAAAGTGAAATCAATCGGCTTTTCCAGCAGCAGCACTGGTCGCGCCAGCGTGGGTGTTATGGTTGAAGGCGAATACACCTTCAGCACCGCTGAGCCGGAAGAGATGACGGTAATCAGTGGCGCGCTGAATGTGTTACTGCCTGACGCGACCGACTGGCAGGTGTATGAAGCCGGTTCGGTGTTTAATGTTCCCGGTCACAGTGAGTTTCATCTGCAAGTTGCCGAACCCACCTCTTATCTGTGCCGCTATCTGTAATTCCTCGCCTTCCCCTTGAACGGGAGGGCATTTTTCTGAAATATCCTTTCTTTAGCCCATAATAATATTTCCTTTGCTGCGATTTTTTCAATTTCCGATATATTCATAA  >  NC_000913/407980‑408570
                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                     
gTCATCATGCTGGATTGTTTAGGATTTCACCAACGTCATCGCGATTTACTGCAAAAACAGCTCGATGTTCCTGTCTTGCTGTCTAACGTATTGATTGCACGGCTGGCTGCGGAATTACTGGTGTAATTTTGCGTGACAGCCAGCGCCTCTGGCCCCTATAGTGAAGTAGATGTTCAACTACCAAACAGGGCCAGTTTATGCTTCAAAGTAATGAGTACTTTTCCGGCAAAGTGAAATCAATCGGCTTTTCCAGCAGCAGCACTGGTCGCGCCAGCGTGGGTGTTATGGTTGAAGGCGAATa                                                                                                                                                                                                                                                                                                    >  2:581989/1‑301 (MQ=255)
      ttGCTGTATTGTTTAGGATTTCACCAACGTCATCGCTATTTACTTCAAAAACAGCTCTATGTTCCTGTCTTGCTGTCTAACGTATTGATTGCACGGCTGGCTGCGGAATTACTGGTGTAATTTTGCGTGACAGCCAGCGCCTCTGGCGCCTATAGTGAAGTAGATGTTCAACTACCAAACATGGCCAGTGTATGCTTCAAAGTAATGAGTACTTTTCCGGCAAAGTGAAATCAATCGGCTTTTCCAGCAGCAGCACTGGTCGCGCCAGCGTGGGTGTTATGGTTGAAGGCGAATACACCtt                                                                                                                                                                                                                                                                                              <  2:520397/300‑1 (MQ=255)
        tcTGTATTGTTTAGGATTTCACCAACGTCATCGCGATTTACTGCAAAAACAGCTCGATGTTCCTGTCTTGCTGTCTAACGTATTGATTGCACGGCTGGCTGCGGAATTACTGGTGTAATTTTGCGTGACAGCCAGCGCCTCTGGCCCCTATAGTGAAGTAGATGTTCAACTACCAAACAGGGCCAGTTTATGCTTCAAAGTAATGAGTACTTTTCCGGCAAAGTGAAATCAATCGGCTTTTCCAGCAGCAGCACTGGTCGCGCCAGCGTGGGTGTTATGGTTGAAGGCGAATACACCTTCa                                                                                                                                                                                                                                                                                            <  2:22528/300‑1 (MQ=255)
            ttccgatttggATTTCACCAACGTCATCGCGATTTACTGCAAAAACAGCTCGATGTTCCTGTCTTGCTGTCTAACGTATTGATTGCACGGCTGGCTGCGGAATTACTGGTGTAATTTTGCGTGACAGCCAGCGCCTCTGGCCCCTATAGTGAAGTAGATGTTCAACTACCAAACAGGGCCAGTTTATGCTTCAAAGTAATGAGTACTTTTCCGGCAAAGTGAAATCAATCGGCTTTTCCAGCAGCAGCACTGGTCGCGCCAGCGTGGGTGTTATGGTTGAAGGTGTATTCGCCTTCaacca                                                                                                                                                                                                                                                                                        <  1:256256/292‑5 (MQ=255)
                     ggATTTCACCAACGTCATCGCGATTTACTGCAAAAACAGCTCGATGTTCCTGTCTTGCTGTCTAACGTATTGATTGCACGGCTGGCTGCGGAATTACTGGTGTAATTTTGCGTGACAGCCAGCGCCTCTGGCCCCTATAGTGAAGTAGATGTTCAACTACCAAACAGGGCCAGTTTATGCTTCAAAGTAATGAGTACTTTTCCGGCAAAGTGAAATCAATCGGCTTTTCCAGCAGCAGCACTGGTCGCGCCAGCGTGGGTGTTATGGTTGAAGGTGTATTCGCCTTCaaccaagatcggaa                                                                                                                                                                                                                                                                               >  2:256524/1‑288 (MQ=255)
                       ttttCACCTACTTCATGGCGATTTATTGCAAAAACTGCTCGTTGTTTCTGTCTTTCTTTCTAACGTATTGATTGCACGGCTGGCTGCGGAATTACTGGTGTAATTTTGCGTGACAGTCAGCGCCTCTGGCACCTATAGTGATGTAGATGTTCAACTACCAAACAGGGCCAGTTTATGCTTCAAAGTAATGAGTACTTTTCCGGCAAAGTGAAATCAATCGGCTTTTCCAGCAGCAGCACTGGTCGCGCCAGCGTGGGTGTTATGGTTGAAGGCGAATACACCTTCAGCACCGCTGAGCCgg                                                                                                                                                                                                                                                                             <  1:443661/300‑1 (MQ=255)
                        tttCACCAACGTCATCGCGATTTACTGCAAAAACAGCTCGATGTTCCTGTCTTGCTGTCTAACGTATTGATTGCACGGCTGGCTGCGGAATTACTGGTGTAATTTTGCGTGACAGCCAGCGCCTCTGGCCCCTATAGTGAAGTAGATGTTCAACTACCAAACAGGGCCAGTTTATGCTTCAAAGTAATGAGTACTTTTCCGGCAAAGTGAAATCAATCGGCTTTTCCAGCAGCAGCACTGGTCGCGCCAGCGTGGGTGTTATGGTTGAAGGCGAATACACCTTCAGCACCGCTGAGCCGGa                                                                                                                                                                                                                                                                            <  2:126093/301‑1 (MQ=255)
                                        tttccgatcTGCAAAAACAGCTCGTTGTTCCTGTCTTTCTGTCTAACGTATTGATTGCACGGCTGGCTGCGGAATTACTGGTGTAATTTTGCGTGACAGCCAGCGCCTCTGGCCCCTATAGTGAAGTAGATGTTCAACTACCAAACAGGGCCAGTTTATGCTTCAAAGTAATGAGTACTTTTCCGGCAAAGTGAAATCAATCGGCTTTTCCAGCAGCAGCACTGGTCGCGCCAGCGTGGGTGTTATGGTTGAAGGCGAATACACCTTCAGCACCGCTGAGCCGGAAGAGATGACGGTAATc                                                                                                                                                                                                                                                            <  2:328163/293‑1 (MQ=255)
                                                  gCAAAAACAGCTCGATGTTCCTGTCTTGCTGTCTAACGTATTGATTGCACGGCTGGCTGCGGAATTACTGGTGTAATTTTGCGTGACAGCCAGCGCCTCTGGCCCCTATAGTGAAGTAGATGTTCAACTACCAAACAGGGCCAGTTTATGCTTCAAAGTAATGAGTACTTTTCCGGCAAAGTGAAATCAATCGGCTTTTCCAGCAGCAGCACTGGTCGCGCCAGCGTGGGTGTTATGGTTGAAGGCGAAAACACCTTCAGCACCGCTGAACCGGAAGAAATGGCGGTAAACAgcaagggag                                                                                                                                                                                                                                                  >  1:327759/1‑293 (MQ=255)
                                                   cAAAAACAGCTCGATGTTCCTGTCTTGCTGTCTAACGTATTGATTGCACGGCTGGCTGCGGAATTACTGGTGTAATTTTGCGTGACAGCCAGCGCCTCTGGCCCCTATAGTGAAGTAGATGTTCAACTACCAAACAGGGCCAGTTTATGCTTCAAAGTAATGAGTACTTTTCCGGCAAAGTGAAATCAATCGGCTTTTCCAGCAGCAGCACTGGTCGCGCCAGCGTGGGTGTTATGGTTGAAGGCGAATACACCTTCAGCACCGCTGAGCCGGAAGAGATGACGGTAATCAGTGGCGCGCt                                                                                                                                                                                                                                                 >  2:448768/1‑301 (MQ=255)
                                                    aaaaaCCGTTCGATTTTCCTGTCTTGCTTTCTAACGTATTGATTGAACGGCTGGCTGCGGAATTACTGGTGTAATTTGGCGTGACAGCCAGCGCCTCTGGCCCCTATAGTGAAGTAGATGTTCAACTACCAAACAGGGCCAGGTTATGCTTCAAAGTAAGGAGTACTTTTCCGGCAAAGTGAAATCAATCGGCTTTTCCAGCAGCAGCACTGGTCGCGCCAGCGTGGGTGTTATGGTTGAAGGCGAATACACCTTCAGCACCGCTGAGCCGGAAGAGATGACGGTAATCAGTGGCGCGCTg                                                                                                                                                                                                                                                <  1:549751/301‑1 (MQ=255)
                                                        aCAGCTCGATGTTCCTGTCTTGCTGTCTAACGTATTGATTGCACGGCTGGCTGCGGAATTACTGGTGTAATTTTGCGTGACAGCCAGCGCCTCTGGCCCCTATAGTGAAGTAGATGTTCAACTACCAAACAGGGCCAGTTTATGCTTCAAAGTAATGAGTACTTTTCCGGCAAAGTGAAATCAATCGGCTTTTCCAGCAGCAGCACTGGTCGCGCCAGCGTGGGTGTTATGGTTGAAGGCGAATACACCTTCAGCACCGCTGAGCCGGAAGAGATGACGGTAATCAGTGCCGCGCAGCAtg                                                                                                                                                                                                                                            >  1:22774/1‑301 (MQ=255)
                                                         aagttgggtgtttctggtgtggtgtgTAACGTGTGGATTGCAGGGGTGGCTGCGGAATTACTGGTGTAATTTTGCGTGACAGCCAGCGCCGCTGGCCACTATAGTGAAGTAGATGTTCAACTACCAAACAGGGCCAGTTTATGCTTCAAAGTAATGAGTACTTTTCCGGCAAAGTGAAATCAATCGGCTTTTCCAGCAGCAGCACTGGTCGCGCCAGCGTGGGTGTTATGGTTGAAGGCGAATACACCTTCAGCACCGCTGAGCCGGAAGAGATGACGGTAATCAGTGGCGCGCTGAAtgt                                                                                                                                                                                                                                           <  1:372791/279‑1 (MQ=255)
                                                                           ttgttgTCGAACGTTTTGATTGCTCGGCTGGCTGCGGAATTACTGGTGTAATTTTGCGTGACGGGCAGCGCCTCTGGCCCCTAAAGTGAAGTAGATTTTCAACTACCAAACAGGGCCAGTTTATGCTTCAAAGTAATGAGTACTTTTCCGGCAAAGTGAAATCAATCGGCTTTTCCAGCAGCAGCACTGGTCGCGCCAGCGTGGGTGTTATGGTTGAAGGCGAATACACCTTCAGCACCGCTGAGCCGGAAGAGATGACGGTAATCAGTGGCGCGCTGAATGTGTTACTGCCTGACGcgac                                                                                                                                                                                                                         <  1:448204/301‑1 (MQ=255)
                                                                                tttttaCGTTTTGATTTCACGGCTGGCTGCGGAATTACTGGTGTAATTTTGCGTGACAGCCAGCGCCTCTGGCCCCTATAGTGAAGTAGATGTTCAACTACCAAACAGGGCCAGTTTATGCTTCAAAGTAATGAGTACTTTTCCGGCAAAGTGAAATCAATCGGCTTTTCCAGCAGCAGCACTGGTCGCGCCAGCGTGGGTGTTATGGTTGAAGGCGAATACACCTTCAGCACCGCTGAGCCGGAAGAGATGACGGTAATCAGTGGCGCGCTGAATGTGTTACTGccagtcggtcgcgtca                                                                                                                                                                                                                    <  2:86462/296‑15 (MQ=255)
                                                                                    aaCGTATTGATTGCACGGCTGGCTGCGGAATTACTGGTGTAATTTTGCGTGACAGCCAGCGCCTCTGGCCCCTATAGTGAAGTAGATGTTCAACTACCAAACAGGGCCAGTTTATGCTTCAAAGTAATGAGTACTTTTCCGGCAAAGTGAAATCAATCGGCTTTTCCAGCAGCAGCACTGGTCGCGCCAGCGTGGGTGTTATGGTTGAAGGCGAATACACCTTCAGCACCGCTGAGCCGGAAGAGATGACGGTAATCAGTGGCGCGATGAATGTGTTACTGCCTGACGCGACCGACTGGCa                                                                                                                                                                                                                >  2:423693/1‑301 (MQ=255)
                                                                                      cGTATTGATTGCACGGCTGGCTGCGGAATTACTGGTGTAATTTTGCGTGACAGCCAGCGCCTCTGGCCCCTATAGTGAAGTAGATGTTCAACTACCAAACAGGGCCAGTTTATGCTTCAAAGTAATGAGTACTTTTCCGGCAAAGTGAAATCAATCGGCTTTTCCAGCAGCAGCACTGGTCGCGCCAGCGTGGGTGTTATGGTTGAAGGCGAATACACCTTCAGCACCGCTGAGCCGGAAGAGATGACGGTAATCAGTGGCGCGCTGAATGTGTTACTGCCTGACGCGACCGACTGGCAgg                                                                                                                                                                                                              >  2:125921/1‑301 (MQ=255)
                                                                                      cGTATTGATTGCACGGCTGGCTGCGGAATTACTGGTGTAATTTTGCGTGACAGCCAGCGCCTCTGGCCCCTATAGTGAAGTAGATGTTCAACTACCAAACAGGGCCAGTTTATGCTTCAAAGTAATGAGTACTTTTCCGGCAAAGTGAAATCAATCGGCTTTTCCAGCAGCAGCACTGGTCGCGCCAGCGTGGGTGTTATGGTTGAAGGCGAATACACCTTCAGCACCGCTGAGCCGGAAGAGATGACGGTAATCAGTGGCGCGCTGAATGTGTTACTGCCTGACGCGACCGACTGGCAgg                                                                                                                                                                                                              >  1:286851/1‑301 (MQ=255)
                                                                                        tATTGATTGCACGGCTGGCTGCGGAATTACTGGTGTAATTTTGCGTGACAGCCAGCGCCTCTGGCCCCTATAGTGAAGTAGATGTTCAACTACCAAACAGGGCCAGTTCATGCTTCAAAGTAATGAGTACTTTTCCGGCAAAGTGGAATCAATCGGCTTTTCCAGCAGCAGCACTGGTCGCGCCAGCGTGGGTGTTATGGTTGAAGGCGAATACACCTTCAGCACCGCTGAGCCGGAAGAGATGACGGTAATCAGTGGCGCGCTGAATGTGTTACTGCCTGACGCGACCGACTGGCAGgtg                                                                                                                                                                                                            <  2:535545/301‑1 (MQ=255)
                                                                                            gattgCACGGCTGGCTGCGGAATTACTGGTGTAATTTTGCGTGACAGCCAGCGCCTCTGGCCCCTATAGTGAAGTAGATGTTCAACTACCAAACAGGGCCAGTTTATGCTTCAAAGTAATGAGTACTTTTCCGGCAAAGTGAAATCAATCGGCTTTTCCAGCAGCAGCACTGGTCGCGCCAGCGTGGGTGTTATGGTTGAAGGCGAATACACCTTCAGCACCGCTGAGCCGGAAGAGATGACGGTAATCAGTGGCGCGCTGAATGTGTTACTGccagtcggtcgcgtcaggcagtaacaca                                                                                                                                                                                                        <  2:389337/301‑27 (MQ=255)
                                                                                                gCAGGGCTGGCTGCGGAATTACTGGTGTAATTTTGCTTGACAGCCAGCGCTTCTGGCCCCTATAGTGAAGTAGATGTTCAACTACCAAACAGGGCCAGTTTATGCTTCAAAGTAATGAGTACTTTTCCGGCAAAGTGAAATCAATCGGCTTTTCCAGCAGCAGCACTGGTCGCGCCAGCGTGGGTGTTATGGTTGAAGGCGAATACACCTTCAGCACCGCTGAGCCGGAAGAGATGACGGTAATCAGTGGCGCGCTGAATGTGTTACTGCCTGACGCGACCGACTGGCAGGTGTATGAAGc                                                                                                                                                                                                    <  1:219256/301‑1 (MQ=255)
                                                                                                gCACGGCTGGCTGCGGAATTACTGGTGTAATTTTGCGTGACAGCCAGCGCCTCTGGCCCCTATAGTGAAGTAGATGTTCAACTACCAAACAGGGCCAGTTTATGCTTCAAAGTAATGAGTACTTTTCCGGCAAAGTGAAATCAATCGGCTTTTCCAGCAGCAGCACTGGTCGCGCCAGCGTGGGTGTTATGGTTGAAGGCGAATACACCTTCAGCACCGCTGAGCAGGAAGAGATGACGGTAATCAGTGGCGCGCTGAATGTGTAACTGCCTGACGCGACCGACTGGAAGATGAATGAAga                                                                                                                                                                                                    >  2:440332/1‑300 (MQ=255)
                                                                                                         gttggggAATTTCTGGTGTAATTTTGCGTGACAGCCAGCGCCTTTGGCCCCTATAGTGAAGTAGATGTTCAACGACCAAACAGGGCCAGTTTATGCTTCAAAGTAATGAGTACTTTTCCGGCAAAGTGAAATCAATCGGCGTTTCCGGCGGCAGTACTGGTCGCGCCAGCGTGGGTGTTATGGTTGAAGGCGAATACACCTTCAGCACCGCTGAGCCGGAAGAGATGACGGTAAGCAGTGGCGCGCTGAATGTGTTACTGCCTGACGCGACCGACTGGCAGGTGTATGAAGCCGGTTCGgt                                                                                                                                                                                           <  1:477354/296‑1 (MQ=255)
                                                                                                          ttgCGGAATTACTGGTGTTATTTTGCGTGACAGCCAGCGCCTCTGGCCCCTATAGTGAAGTAGATGTTCAACTACCAAACAGGGCCAGTTTATGCTTCAAAGTAATGAGTACTTTTCCGGCAAAGTGAAATCAATCGGCTTTTCCAGCAGCAGCACTGGTCGCGCCAGCGTGGGTGTTATGGTTGAAGGCGAATACACCTTCAGCACCGCTGAGCCGGAAGAGATGACGGTAATCAGTGGCGCGCTGAATGTGTTACTGCCTGACGCGACCGACTGGCAGGTGTATGAAGCCGGTTCGgtg                                                                                                                                                                                          <  2:520121/300‑1 (MQ=255)
                                                                                                                    cagtcgtttgctcttccgatctgccagcGCCTCTGGCCCCTATAGTGAAGTAGATGTTCAACTACCAAACAGGGCCAGTTTATGCTTCAAAGTAATGAGTACTTTTCCGGCAAAGTGAAATCAATCGGCTTTTCCAGCAGCAGCACTGGTCGCGCCAGCGTGGGTGTTATGGTTGAAGGCGAATACACCTTCAGCACCGCTGAGCCGGAAGAGATGACGGTAATCAGTGGCGCGCTGAATGTGTTACTGCCTGACGCGACCGACTGGCAGGTGTATGAAGCCGGTTCGGTGTTTAATGTTc                                                                                                                                                                                <  2:201127/279‑1 (MQ=255)
                                                                                                                       ggTGTAATTTTGCGTGACAGCCAGCGCCTCTGGCCCCTATAGTGAAGTAGATGTTCAACTACCAAACAGGGCCAGTTTATGCTTCAAAGTAATGAGTACTTTTCCGGCAAAGTGAAATCAATCGGCTTTTCCAGCAGCAGCACTGGTCGCGCCAGCGTGGGTGTTATGGTTGAAGGCGAATACACCTTCAGCACCGCTGAGCCGGAAGAGATGACGGTAATCAGTGGCGCGCTGAATGTGTTACTGCCTGACGCGACCGACTGGCAGGTGTATGAAGCCGGTTCGGTGTTTAATGTTCCCg                                                                                                                                                                             >  2:548769/1‑301 (MQ=255)
                                                                                                                        gtgtAATTTTGCGTGACAGCCAGCGCCTCTGGCCCCTATAGTGAAGTAGATGTTCAACTACCAAACAGGGCCAGTTTATGCTTCAAAGTAATGAGTACTTTTCCGGCAAAGTGAAATCAATCGGCTTTTCCAGCAGCAGCACTGGTCGCGCCAGCGTGGGTGTTATGGTTGAAGGCGAATACACCTTCAGCACCGCTGAGCCGGAAGAGATGACGGTAATCAGTGGCGCGCTGAATGTGTTACTGCCTGACGCGACCGACTGGCAGGTGTATGAAGCCGGTTCGGTATTTAATGTTCCCgg                                                                                                                                                                            >  1:51404/1‑301 (MQ=255)
                                                                                                                           tAATTTTGCGTGACAGCCAGCGCCTCTGGCCCCTATAGTGAAGTAGATGTTCAACTACCAAACAGGGCCAGTTTATGCTTCAAAGTAATGAGTACTTTTCCGGCAAAGTGAAATCAATCGGCTTTTCCAGCAGCAGCACTGGTCGCGCCAGCGTGGGTGTTATGGTTGAAGGCGAATACACCTTCAGCACCGCTGAGCCGGAAGAGATGACGGTAATCAGTGGCGCGCTGAATGTGTTACTGCCTGACGCGACCGACTGGCAGGTGTATGAAGCCGATTCGGTGTTTAATTTTCCCGGTca                                                                                                                                                                         >  2:19473/1‑301 (MQ=255)
                                                                                                                                ttGCGGGACAGCCAGCGCCTGTGGCCCCTATAGTGAAGTAGATGTTCAACTACCAAACAGGGCCAGTTTATGCTTCAAAGTAATGAGTACTTTTCCGGCAAAGTGAAATCAATCGGCTTGTCCAGCAGCAGCACTGGTCGCGCCAGCGTGGGTGTTATGGTTGAAGGCGAATACACCTTCAGCACCGCTGAGCCGGAAGAGATGACGGTAATCAGTGGCGCGCTGAATGTGTTACTGCCTGACGCGACCGACTGGCAGGTGTATGAAGCCGGTTCGGTGTTTAATGTTCCCGGTCACAGTg                                                                                                                                                                    <  1:548096/301‑1 (MQ=255)
                                                                                                                                    gTGACAGCCAGCGCCTCTGGCCCCTATAGTGAAGTAGATGTTCAACTACCAAACAGGGCCAGTTTATGCTTCAAAGTAATGAGTACTTTTCCGGCAAAGTGAAATCAATCGGCTTTTCCAGCAGCAGCACTGGTCGCGCCAGCGTGGGTGTTATGGTTGAAGGCGAATACACCTTCAGCACCGCTGAGCCGGAAGAGATGACGGTAATCAGTGGCGCGCTGAATGTGTTACTGCCTGACGCGACCGACTGGCAGGTGTATGAAGCCGGTTCGGTGTTTAATGTTCCCGGTCACAGTGAGtt                                                                                                                                                                >  2:54804/1‑301 (MQ=255)
                                                                                                                                     tGACAGCCAGCGCCTTTGGCCCCTATAGTTAAGTTGATGTTCAACTTCCAAACAGGGCCAGTTTATGCTTCAAAGTAATGAGTACTTTTCCGGCAAAGTGAAATCAATCGGCTTTTCCAGCAGCAGCACTGGTCGCGCCAGCGTGGGTGTTATGGTTGAAGGCGAATACACCTTCAGCACCGCTGAGCCGGAAGAGATGACGGTAATCAGTGGCGCGCTGAATGTGTTACTGCCTGACGCGACCGACTGGCAGGTGTATGAAGCCGGTTCGGTGTTTAATGTTCCCGGTCACAGTGAGttt                                                                                                                                                               <  1:439768/301‑1 (MQ=255)
                                                                                                                                     tGACAGCCAGCGCCTCTGGCCCCTATAGTGAAGTAGATGTTCAACTACCAAACAGGGCCAGTTTATGCTTCAAAGTAATGAGTACTTTTCCGGCAAAGTGAAATCAATCGGCTTTTCCAGCAGCAGCACTGGTCGCGCCAGCGTGGGTGTTATGGTTGAAGGCGAATACACCTTCAGCACCGCTGAGCCGGAAGAGATGACGGTAATCAGTGGCGCGCTGACTGTGTTACTGCCTGACGCGACCGACTGGCAGGTGTATGAAGCCGGTTCGGGGTTTAATGTTCCAGGTCACAGTGAGttt                                                                                                                                                               >  1:89841/1‑301 (MQ=255)
                                                                                                                                     ggACAGCCAGCGCCTCTGGCCCCTTTAGTGAAGTAGATGTTCAACTACCAAACAGGGCCAGTTTATGCTTCAAAGTAATGAGTACTTTTCCGGCAAAGTGAAATCAATCGGCTTTTCCAGCAGCAGCACTGGTCGCGCCAGCGTGGGTGTTATGGTTGAAGGCGAATACACCTTCAGCACCGCTGAGCCGGAAGAGATGACGGTAATCAGTGGCGCGCTGAATGTGTTACTGCCTGACGCGACCGACTGGCAGGTGTATGAAGCCGGTTCGGTGTTTAATGTTCCCGGTCACAGTGAGttt                                                                                                                                                               <  1:125797/300‑1 (MQ=255)
                                                                                                                                          gccagcGCCTCTGGCCCCTATAGTGAAGTAGATGTTCAACTACCAAACAGGGCCAGTTTATGCTTCAAAGTAATGAGTACTTTTCCGGCAAAGTGAAATCAATCGGCTTTTCCAGCAGCAGCACTGGTCGCGCCAGCGTGGGTGTTATGGTTGAAGGCGAATACACCTTCAGCACCGCTGAGCCGGAAGAGATGACGGTAATCAGTGGCGCGCTGAATGTGTTACTGCCTGACGCGACCGACTGGCAGGTGTATGAAGCCGGTTCGGTGTTTAATGttaagaacggaagagcgtcgtgtag                                                                                                                                                          >  1:200884/1‑278 (MQ=255)
                                                                                                                                                           ttttaGTGAAGTAGTTGTTCAACTACCAAACAGGGCCAGTTTATGCTTCAAAGTAATGAGTACTTTTCCGGCAAAGTGAAATCAATCGGCTTTTCCAGCAGCAGCACTGGTCGCGCCAGCGTGGGTGTTATGGTTGAAGGCGAATACACCTTCAGCACCGCTGAGCCGGAAGAGATGACGGTAATCAGTGGCGCGCTGAATGTGTTACTGCCTGACGCGACCGACTGGCAGGTGTATGAAGCCGGTTCGGTGTTTAATGTTCCCGGTCACAGTGAGTTTCATCTGCAAGTTGCCGAACCCa                                                                                                                                         <  1:525966/298‑1 (MQ=255)
                                                                                                                                                             tgctcttccgatctGTTCAACTACCAAACAGGGCCAGTTTATGCTTCAAAGTAATGAGTACTTTTCCGGCAAAGTGAAATCAATCGGCTTTTCCAGCAGCAGCACTGGTCGCGCCAGCGTGGGTGTTATGGTTGAAGGCGAATACACCTTCAGCACCGCTGAGCCGGAAGAGATGACGGTAATCAGTGGCGCGCTGAATGTGTTACTGCCTGACGCGACCGACTGGCAGGTGTATGAAGCCGGTTCGGTGTTTAATGTTCCCGGTCACAGTGAGTTTCATCTGCAAGTTGCCGAACCCAcc                                                                                                                                       <  2:390047/288‑1 (MQ=255)
                                                                                                                                                                ggtaaGTAGATGTTCAACTACCAAACAGGGCCAGTTTATGCTTCAAAGTAATGAGTACTTTTCCGGCAAAGTGAAATCAATCGGCTTTTCCAGCAGCAGCACTGGTCGCGCCAGCGTGGGTGTTATGGTTGAAGGCGAATACACCTTCAGCACCGCTGAGCCGGAAGAGATGACGGTAATCAGTGGCGCGCTGAATGTGTTACTGCCTGACGCGACCGACTGGCAGGTGTATGAAGCCGGTTCGGTGTTTAATGTTCCCGGTCACAGTGAGTTTCATCTGCAAGTTGCCGAACCCACctct                                                                                                                                    <  1:581265/298‑1 (MQ=255)
                                                                                                                                                                  gAAGTAGATGTTCAACTACCAAACAGGGCCAGTTTATGCTTCAAAGTAATGAGTACTTTTCCGGCAAAGTGAAATCAATCGGCTTTTCCAGCAGCAGCACTGGTCGCGCCAGCGTGGGTGTTATGGTTGAAGGCGAATACACCTTCAGCACCGCTGAGCCGGAAGAGATGACGGTAATCAGTGGCGCGCTGAATGTGTTACTGCCTGACGCGACCGACTGGCAGGTGTATGAAGCCGGTTCGGTGTTTAATGTTCCCGGTCACAGTGAGTTTCATCTGCAAGTTGCCGAACCAACctctaa                                                                                                                                  >  2:624692/1‑299 (MQ=255)
                                                                                                                                                                          ttctCAACTACCAAACAGGGCCAGTTTATGCTTCAAAGTAATGAGTACTTTTCCGGCAAAGTGAAATCAATCTGCTTTTCCAGCAGCAGCACTGGTCGCGCCAGCGTGGGTGTTATGGTTGAAGGCGAATACACCGTCAGCACCGCTGAGCCGGAAGAGATGACGGTAATCAGTGGCGCGCTGAATGTGTTACTGCCTGACGCGACCGACTGTCAGGTGTATGAAGCCGGTTCGGTGTTTAATGTTCCCGGTCACAGTGAGTTTCATCTGCAAGTTGCCGAACCCACCTCTTATCTGTGcc                                                                                                                          <  2:355164/298‑1 (MQ=255)
                                                                                                                                                                           tttCAACTACCAAACAGGGCCAGTTTATGCTTCAAAGTAATGAGTACGTTTCCGGCAAAGTGAAATCAATCGGCTTTTCCAGCAGCAGCACTGGTCGCGCCAGCGTGGGTGTTATGGTTGAAGGCGAATACACCTTCAGCACCGCTGAGCCGGAAGAGATGACGGTAATCAGTGGCGCGCTGAATGTGTTACTGCCTGACGCGACCGACTGGCAGGTGTATGAAGCCGGTTCGGTGTTTAATGTTCCCGGTCACAGTGAGTTTCATCTGCAAGTTGCCGAACCCACCTCTTATCTGTGCCg                                                                                                                         <  2:287253/300‑1 (MQ=255)
                                                                                                                                                                           gTTCAACTACCAAACAGGGCCAGTTTATGCTTCAAAGTAATGAGTACTTTTCCGGCAAAGTGAAATCAATCGGCTTTTCCAGCAGCAGCACTGGTCGCGCCAGCGTGGGTGTTATGGTTGAAGGCGAATACACCTTCAGCACCGCTGAGCCGGAAGAGATGACGGTAATCAGTGGCGCGCTGAATGTGTTACTGCCTGACGCGACCGACTGGCAGGTGTATGAAGCCGGTTCGGTGTTTAATGTTCCCGGTCACAGTGAGTTTCATCTGCAAGTTGCCGAAaccacccgatcggaaaagcg                                                                                                                         >  1:389533/1‑287 (MQ=255)
                                                                                                                                                                              cAACTACCAAACAGGGCCTGTTTATGCTTCAAAGTAATGAGTACTTTTCCGGAAAAGTGAAATCAATCGGCATTTCCAGCAGAAGCACATGTCACGCCAGCGTGGGTGTTATGGTTGAAGGCGAATACACCTTCAGCACCGCTGAGACGGAAGAGATGAAGGTAATCAGTGGCGCGCTGACTGTGTTACTGCCTGCCGCGACCAACTGGCAGGTGTATGACGCCGGTTCGGGGTTCAATGTTCACGATAACAGTGAGTTTAATCTGCAAGTTGACGAACCCACCTCTTATCTGTGccagat                                                                                                                      >  1:354680/1‑297 (MQ=255)
                                                                                                                                                                                aCTACCAATCAGGGCCAGTTTATGCTTCAAAGTAATGAGTACTTTTCCGGCAAAGTGAAATCAATCGGCTTTTCCAGCAGCAGCACTGGTCGCGCCAGCGTGGGTGTTATGGTTGAAGGCGAATACACCTTCAGCACCGCTGAGCCGGAAGAGATGACGGTAATCAGTGGCGCGCTGAATGTGTTACTGCCTGACGCGACCGACTGGCAGGTGTATGAAGCCGGTTCGGTGTTTAATGTTCCCGGTCACAGTGAGTTTCATCTGCAAGTTGCCGAACCCACCTCTTATCTGTGCCGCTATc                                                                                                                    <  1:423130/301‑1 (MQ=255)
                                                                                                                                                                                     cAAACAGGGCCAGTTTATGCTTCAAAGTAATGAGTACTTTTCCGGCAAAGTGAAATCAATCGGCTTTTCCAGCAGCAGCACTGGTCGCGCCAGCGTGGGTGTTATGGTTGAAGGCGAATACACCTTCAGCACCGCTGAGCCGGAAGAGATGACGGTAATCAGTGGCGCGCTGAATGTGTTACTGCCTGACGCGACCGACTGGCAGGTGTATGAAGCCGGTTCGGTGTTTAATGTTCCCGGTCACAGTGAGTTTCATCTGCAAGTTGCCGAACCCACCTCTTATCTGTGCCGCTATCTGaaa                                                                                                               >  2:597543/1‑301 (MQ=255)
                                                                                                                                                                                         ataggCCAGTTTATGCTTCAAAGTAAGTAGTACTTTTCCGGCAAAGTGAGATCAATCGGCTGTTCCAGCAGCAGCACTGGTCGCGCCAGCGTGGGTGTTATGGTTGAAGGCGAATACACCTTCAGCACCGCTGAGCTGGAAGAGATGACGGTAATCAGTGGCGCGCTGAATGTGTTACTGCCTGACGCGACCGACTGGCAGGTGTATGAAGCCGGTTCGGTGTTTAATGTTCCCGGTCACAGTGAGTTTCATCTGCAAGTTGCCGAACCCACCTCTTATCTGTGCCGCTATCTGTAATTcc                                                                                                           <  1:599901/298‑1 (MQ=255)
                                                                                                                                                                                                aGTTTATGCTTCAAAGTAATGAGTACTTTTCCGGCAAAGTGAAATCAATCGGCTTTTCCAGCAGCAGCACTGGTCGCGCCAGCGTGGGTGTTATGGTTGAAGGCGAATACACCTTCAGCACCGCTGAGCCGGAAGAGATGACGGTAATCAGTGGCGCGCTGAATGTGTTACTGCCTGACGCGACCGACTGGCAGGTGTATGAAGCCGGTTCGGTGTTTAATGTTCCCGGTCACAGTGAGTTTCATCTGCAAGTTGCCGAACCCACCTCTTATCTGTGCCGCTATCTGTAATTCCTCGCCtt                                                                                                    <  2:51472/301‑1 (MQ=255)
                                                                                                                                                                                                               ttAATGAGTACTTTTCCGGCAAAGTGAAATCAATCGGCTTTTCCAGCAGCAGCACTGGTCGCGCCAGCGTGGGTGTTATGGTTGAAGGCGAATACACCTTCAGCACCGCTGAGCCGGAAGAGATGACGGTAATCAGTGGCGCGCTGAATGTGTTACTGCCTGACGCGACCGACTGGCAGGTGTATGAAGCCGGTTCGGTGTTTAATGTTCCCGGTCACAGTGAGTTTCATCTGCAAGTTGCCGAACCCACCTCTTATCTGTGCCGCTATCTGTAATTCCTCGCCTTCCCCTTGAACGGGAg                                                                                     <  2:22825/300‑1 (MQ=255)
                                                                                                                                                                                                               gttaTGAGTACTTTTCCGGCAAAGTGAAATCAATCGGCTTTTCCAGCAGCAGCACTGGTCGCGCCAGCGTGGGTGTTATGGTTGAAGGCGAATACACCTTCAGCACCGCTGAGCCGGAAGAGATGACGGTAATCAGTGGCGCGCTGAATGTGTTACTGCCTGACGCGACCGACTGGCAGGTGTATGAAGCCGGTTCGGTGTTTAATGTTCCCGGTCACAGTGAGTTTCATCTGCAAGTTGCCGAACCCACCTCTTATCTGTGCCGCTATCTGTAATTCCTCGCCTTCCCCTTGAACGGGAg                                                                                     <  1:596782/298‑1 (MQ=255)
                                                                                                                                                                                                                 aaTGATGACTTTTCCGGCAAAGGTAAATCAATCGGTTTTTCCAGCAGCAGCACTGGTCGCGCCAGCGTGGGTGTTATGGTTGAAGGCGAATACACCTTCAGCACCGCTGAGCCGGAAGAGATGACGGTAATCAGTGGCGCGCTGAATGTGTTACTGCCTGACGCGACCGACTGGCAGGTGTATGAAGCCGGTTCGGTGTTTAATGTTCCCGGTCACAGTGAGTTTCATCTGCAAGTTGCCGAACCCACCTCTTATCTGTGCCGCTATCTGTAATTCCTCGCCTTCCCCTTGAACGGGAggg                                                                                   <  1:222243/301‑1 (MQ=255)
                                                                                                                                                                                                                     aGTACTTTTCCGGCAAAGTGAAATCAATCGGCTTTTCCAGCAGCAGCACTGGTCGCGCCAGCGTGGGTGTTATGGTTGAAGGCGAATACACCTTCAGCACCGCTGAGCCGGAAGAGATGACGGTAATCAGTGGCGCGCTGAATGTGTTACTGCCTGACGCGACCGACTGGCAGGTGTATGAAGCCGGTTCGGTGTTTAATGTTCCCGGTCACAGTGAGTTTCATCTGCAAGTTGCCGAACCCACCTCTTCTCTGTGCCGCTATCTGTAATTCCTCGCCTTCCCCTTGAACGGGAGGGCAtt                                                                               >  2:349436/1‑301 (MQ=255)
                                                                                                                                                                                                                              ccGGCAAAGTGAAATCAATCGGCTTTTCCAGCAGCAGCACTGGTCGCGCCAGCGTGGGTGTTATGGTTGAAGGCGAATACACCTTCAGCACCGCTGAGCCGGAAGAGATGACGGTAATCAGTGGCGCGCTGAATGTGTTACTGCCTGACGCGACCGACTGGCAGGTGTATGAAGCCGGTTCGGTGTTTAATGTTCCCGGTCACAGTGAGTTTCATCTGCAAGTTGCCGAACCCACCTCTTATCTGTGCCGCTATCTGTAATTCCTCGCCTTCCCCTTGAACGGGAGGGCATTTTTCTGaaa                                                                      <  2:565362/301‑1 (MQ=255)
                                                                                                                                                                                                                                                 gtgCTTTTCCAGCGGCAGAACTGGGTGCGCCAGCGTGGGTGTGATGGTTGAAGGGGAATACACCTTCAGCACCGCTGAGCCGGAAGAGGTGACGGTAATCAGTGGCGCGCTGAATGTGTTACTGCCTGACGCGACCGACTGGCAGGTGTATGAAGCCGGTTCGGTGTTTAATGTTCCCGGTCACAGTGAGTTTCATCTGCAAGTTGCCGAACCCACCTCTTATCTGTGCCGCTATCTGTAATTCCTCGCCTTCCCCTTGAACGGGAGGGCATTTTTCTGAAATATCCTTTCTTTAGCCCat                                                   <  1:623833/299‑1 (MQ=255)
                                                                                                                                                                                                                                                  ggCTTTTCCAGCAGCAGCACTGGTCGCGCCAGCGTGGTTGTTATGGTTGAAGGCGAATACACCTTCAGCACCGCTGAGCCGGAAGAGATGACGGTAATCAGTGGCGCGCTGAATGTGTTACTGCCTGACGCGACCGACTGGCAGGTGTATGAAGCCGGTTCGGTGTTTAATGTTCCCGGTCACAGTGAGTTTCATCTGCAAGTTGCCGAACCCACCTCTTATCTGTGCCGCTATCTGTAATTCCTCGCCTTCCCCTTGAACGGGAGGGCATTTTTCTGAAATATCCTTTCTTTAGCCCata                                                  <  1:54736/301‑1 (MQ=255)
                                                                                                                                                                                                                                                               gcagcaCTGGTCGCGCCAGCGCGGGTGTTATGGTTGAAGGCGAATACACCTTCAGCACCGCTGAGCCGGCAGAGATGACGGTAATCAGTGGCGCGCTGAATGTGTTACTGCCTGACGCGACCGACTGGCAGGTGTATGAAGCCGGTTCGGTGTTTAACGTTCCCGGTCACAGTGAGTTTCATCTGCACGTTGCCGAACCCACCCCTTCTCTGTGCCGCTATCTGTCATTCCTCGCCTTCCCCTTGAACGGGAGGGCATTTTTCTGAAATCTCCTTTCTTTAGCCCATAATAATATTTCCtt                                     >  1:558924/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                       ggTCGCGCCAGCGTGGGTGTTATGGTTGAAGGCGAATACACCTTCAGCACCGCTGAGCCGGAAGAGATGACGGTAATCAGTGGCGCGCTGAATGTGTTACTGCCTGACGCGACCGACTGGCAGGTGTATGAAGCCGGTTCGGTGTTTAATGTTCCCGGTCACAGTGAGTTTCATCTGCAAGTTGCCGAACCCACCTCTTATCTGTGCCGCTATCTGTAATTCCTCGCCTTCCCCTTGAACGGGAGGGCATTTTTCTGAAATATCCTTTCTTTAGCCCATAATAATATTTCCTTTGCTGCGa                             >  2:512385/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                             acgacgctcttccgatctgctGAAGGTGTATTCGCCTTCAGCACCGCTGAGCCGGAAGAGATGACGGTAATCAGTGGCGCTCTGAATGTGTTACTGCCTGACGCGACCGACTGGCAGGTGTATGAAGCCGGTTCGGTGTTTAATGTTCCCGGTCACAGTGAGTTTCATCTGCAAGTTGCCGAACCCACCTCTTATCTGTGCCGCTATCTGTAATTCCTCGCCTTCCCCTTGAACGGGAGGGCATTTTTCTGAAATATCCTTTCTTTAGCCCATAATAATATTTCCTTTGCTGCGAtttttt                       <  1:367536/281‑1 (MQ=255)
                                                                                                                                                                                                                                                                                             tGGTTGAAGGCGAATACACCTTCAGCACCGCTGAGCCGGAAGAGATGACGGTAATCAGTGGCGCGCTGAATGTGTTACTGCCTGACGCGACCGACTGGCAGGTGTATGAAGCCGGTTCGGTGTTTAATGTTCCCGGTCACAGTGAGTTTCATCTGCAAGTTGCCGAACCCACCTCTTATCTGTGCCGCTATCTGTAATTCCTCGCCTTCCCCTTGAACGGGAGGGCATTTTTCTGAAATATCCTTTCTTTAGCCCATAATAATATTTCCTTTGCTGCGATTTTTTCAATTTCCGATATAtt       >  1:61994/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                                               gctGAAGGTGTATTCGCCTTCAGCACCGCTGAGCCGGAAGAGATGACGGTAATCAGTGGCGCGCTGAATGTGTTACTGCCTGACGCGACCGACTGGCAGGTGTATGAAGCCGGTTCGGTGTTTAATGTTCCCGGTCACAGTGAGTTTCATCTGCAAGTTGCCGAACCCACCTCTTATCTGTGCCGCTATCTGTAATTCCTCGCCTTCCCCTTGAACGGGAGGGCATTTTTCTGAAATATCCTTTCTTTAGCCCATAATAATATTTCCTTTGCTGCGAttttttagatcggaagagcacaca     >  2:368020/3‑283 (MQ=255)
                                                                                                                                                                                                                                                                                                  gAAGGCGAATACACCTTCAGCACCGCTGAGCCGGAAGAGATGACGGTAATCAGTGGCTCGCTGAATGTGTTACTGCCTGACGCGACCGACTGGCAGGTGTATGAAGCAGGTTCGGTGTTTAATGTTCCCGGTCACAGTGCGTTTCATCTGCAAGTTGCCGAACCCACCTCTTATCTGTGCCGCTATCTGTAATTCCTCGACTTCCCCTTTAACGGGAGGGCATTTATCTGTAATATCCTTTCTTTAGCACATAATAAAATTTCCATAGCTCCGAATTTTTACATTTCCAATATATTCaaaa  >  1:474430/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                     
GTCATCATGCTGGATTGTTTAGGATTTCACCAACGTCATCGCGATTTACTGCAAAAACAGCTCGATGTTCCTGTCTTGCTGTCTAACGTATTGATTGCACGGCTGGCTGCGGAATTACTGGTGTAATTTTGCGTGACAGCCAGCGCCTCTGGCCCCTATAGTGAAGTAGATGTTCAACTACCAAACAGGGCCAGTTTATGCTTCAAAGTAATGAGTACTTTTCCGGCAAAGTGAAATCAATCGGCTTTTCCAGCAGCAGCACTGGTCGCGCCAGCGTGGGTGTTATGGTTGAAGGCGAATACACCTTCAGCACCGCTGAGCCGGAAGAGATGACGGTAATCAGTGGCGCGCTGAATGTGTTACTGCCTGACGCGACCGACTGGCAGGTGTATGAAGCCGGTTCGGTGTTTAATGTTCCCGGTCACAGTGAGTTTCATCTGCAAGTTGCCGAACCCACCTCTTATCTGTGCCGCTATCTGTAATTCCTCGCCTTCCCCTTGAACGGGAGGGCATTTTTCTGAAATATCCTTTCTTTAGCCCATAATAATATTTCCTTTGCTGCGATTTTTTCAATTTCCGATATATTCATAA  >  NC_000913/407980‑408570

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑