Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 407,248 T→G 7.3% intergenic (+78/‑180) yaiA → / → aroM OxyR‑regulated conserved protein/AroM family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913407,2480TG 7.3% 171.1 / 3.9 55intergenic (+78/‑180)yaiA/aroMOxyR‑regulated conserved protein/AroM family protein
Reads supporting (aligned to +/- strand):  ref base T (26/25);  new base G (2/2);  total (28/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.33e-01

ACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGATTGAAAAAGGAAAGCCAGGACAGACGGTAACCTGGTACCAACTCAGAGCCGATCATCCTAAACCAGACTCGTTGATCAGTGAACATCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACAAGGGATCTGCGTCACATTTTTCATAATTCATGTTTTTCTAATAATTAGAATATTAAACAATAACAATCCATTACTGGAATCATTTGGAATCTTTACATTATGCCGTGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGCAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTGGCGATCCTGACCATCGGCATTGTACCTATGCAGGAAGTTTTGCCGCTCCTGACGGAATACATTGACGAAGATAATATTTCCCATCATAGCCTGCTGGGGAAGTTA  >  NC_000913/406950‑407547
                                                                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                                                           
aCGTTCATCCCGTGAAATAAGGAAGAACGTTGCAAACGAAACCGCCTTATCCTCGGGAAGCATATATAGGGACGATTGAAAAAGGAAAGCCAGGACAGACGGTAACCTGGTACCAACTCAGAGCCGATCATCCTAAACCAGACTCGTTGATCAGTGAACATCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACAAGGGATCTGCGTCACATTTTTCATAATTCATGTTTTTCt                                                                                                                                                                                                                                                                                                           <  1:557701/301‑1 (MQ=255)
                 tcaGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCGTATATAGTGACGATTGAAACAGGAAAGCCAGGACAGACGGTAACCTGGTACCAACTCAGAGCCGATCATCCTAAACCCGACTCGTTGATCAGTGAACATCCGACCGCTCAGGACGCGCAGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGAACGCATCAGACTGCTCGGCAGGGATTCTGAGTGCCACTACACGGGATCTGCGTCACATTTTTAATAATTCATGTTTTTCTAATACTTAGAATATTaa                                                                                                                                                                                                                                                                                          >  2:537280/3‑301 (MQ=255)
                 tAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCGTATATAGTGACGATTGAAAAAGGAAAGCCAGGACAGACGGTAACCTGGTACCAACTCAGAGCCGATCATCCTAAACCAGACTCGTTGATCAGTGAACATCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACAAGGGATCTGCGTCACATTTTTCATAATTCATGTTTTTCTAATAATTAGAATATTaa                                                                                                                                                                                                                                                                                          >  2:537268/1‑301 (MQ=255)
                 acagtaagaaCGATGCCCACGAAACCGCCTTATCCTCGGGAAGCATATATAGTGACGATTGAAAAAGGAAAGCCAGGACAGACGGTAACCTGGTACCAACTCAGAGCCGATCATCCTAAACCAGACTCGTTGATCAGTGAACATCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACAAGGGATCTGCGTCACATTTTTCATAATTCATGTTTTTCTAATAATTAGAATATTaa                                                                                                                                                                                                                                                                                          <  1:400584/296‑1 (MQ=255)
                                              ttATCCTCGTGAAGCATATATAGTGACGATTGAAAAAGGAAAGCCAGGACAGACGGTAACCTGGTACCAACTCAGAGCCGATCATCCTAAACCAGACTCGTTGATCAGTGAACATCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACAAGGGATCTGCGTCACATTTTTCATAATTCATGTTTTTCTAATAATTAGAATATTAAACAATAACAATCCATTACTGGAATCAttt                                                                                                                                                                                                                                                             <  2:299249/301‑1 (MQ=255)
                                                                     ctttccctacacgacgctcttccgatctgACGGTAACCTGGTACCAACTCAGAGCCGATCATCCTAAACCAGACTCGTTGATCAGTGAACATCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACAAGGGATCTGCGTCACATTTTTCATAATTCATGTTTTTCTAATAATTAGAATATTAAACAATAACAATCCATTACTGGAATCATTTGGAATCTTTACATTATGCCGTGc                                                                                                                                                                                                                                      <  1:143168/273‑1 (MQ=255)
                                                                            agAAAAAGGAAAGCCAGGACAGACGGTAACCTGGTACCAACTCAGAGCCGATCATCCTAAACCAGACTCGTTGATCAGTGAACATCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACAAGGGATCTGCGTCACATTTTTCATAATTCATGTTTTTCTAATAATTAGAATATTAAACAATAACAATCCATTACTGGAATCATTTGGAATCTTTACATTATGCCGTGCACGTctg                                                                                                                                                                                                                               <  1:68228/300‑1 (MQ=255)
                                                                             gAAAAAGGAAAGCCAGGACAGACGGTAACCTGGTACCAACTCAGAGCCGATCATCCTAAACCAGACTCGTTGATCAGTGAACATCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACAAGGGATCTGCGTCACATTTTTCATAATTCATGTTTTTCTAATAATTAGAATATTAAACAATAACAATCCATTACTGGAATCATTTGGAATCTTTACATAATGCCGTGCACGTctgc                                                                                                                                                                                                                              >  1:526028/1‑301 (MQ=255)
                                                                             gAAAAAGGAAAGCCAGGACAGACGGTAACCTGGTACCAACTCAGAGCCGATCATCCTAAACCAGACTCGTTGATCAGTGAACATCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACAAGGGATCTGCGTCACATTTTTCATAATTCATGTTTTTCTAATAATTAGAATATTAAACAATAACAATCCATTACTGGAATCATTTGGAATCTTTAAATTATGCCGTGCACGTctgc                                                                                                                                                                                                                              >  2:153558/1‑301 (MQ=255)
                                                                              aaaaaGGAAAGCCAGGACAGACGGTAACCTGGTACCAACTCAGAGCCGATCATCCTAAACCAGACTCGTTGATCAGTGAACATCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACAAGGGATCTGCGTCACATTTTTCATAATTCATGTTTTTCTAATAATTAGAATATTAAACAATAACAATCCATTACTGGAATCATTTGGAATCTTTACATTATGCCGTGCACGTctgct                                                                                                                                                                                                                             <  1:153407/301‑1 (MQ=255)
                                                                               aaaaGGAAAGCCAGGACAGACGGTAACCTGGTACCAACTCAGAGCCGATCATCCTAAACCAGACTCGTTGATCAGTGAACATCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACAAGGGATCTGCGTCACATTTTTCATAATTCATGTTTTTCTAATAATTAGAATATTAAACAATAACAATCCATTACTGGAATCATTTGGAATCTTTACATTAAGCCGTACAAGTctgctg                                                                                                                                                                                                                            >  1:140740/1‑301 (MQ=255)
                                                                                          aaGGACAGACGGTAACCTGGTACCAACTCAGAGCCGATCATCCTAAACCAGACTCGTTGATCAGTGAACATCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACAAGGGATCTGCGTCACATTTTTCATAATTCATGTTTTTCTAATAATTAGAATATTAAACAATAACAATCCATTACTGGAATCATTTGGAATCTTTACATTATGCCGTGCACGTCTGCTGCTACGCttttt                                                                                                                                                                                                                 <  1:509057/300‑1 (MQ=255)
                                                                                             gACAGACGGTAACCTGGTACCAACTCAGAGCCGATCATCCTAAACCAGACTCGTTGATCAGTGAACATCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACAAGGGATCTGCGTCACATTTTTCATAATTCATGTTTTTCTAATAATTAGAATATTAAACAATAACAATCCATTACTGGAATCATTTGGAATCTTTACATTATGCCGTGCACGTCTGCTGCTACGCTTTTTgat                                                                                                                                                                                                              >  1:214909/1‑299 (MQ=255)
                                                                                               cAGACGGTAACCTGGTACCAACTCAGAGCCGATCATCCTAAACCAGACTCGTTGATCAGTGAACATCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACAAGGGATCTGCGTCACATTTTTCATAATTCATGTTTTTCTAATAATTAGAATATTAAACAATAACAATCCATTACTGGAATCATTTGGAATCTTTACATTATGCCGTACACGTCTGCTGATACGATATTTTTCAt                                                                                                                                                                                                            >  2:590639/1‑301 (MQ=255)
                                                                                                 gACGGTAACCTGGTACCAACTCAGAGCCGATCATCCTAAACCAGACTCGTTGATCAGTGAACATCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACAAGGGATCTGCGTCACATTTTTCATAATTCATGTTTTTCTAATAATTAGAATATTAAACAATAACAATCCATTACTGGAATCATTTGGAATCTTTACATTATGCCGTGCagatcggaagagcacacgtctgaactcc                                                                                                                                                                                                          >  2:143294/1‑274 (MQ=255)
                                                                                                                      aaaggccggctctcCTAAACCAGGATCGGTGATCAGGGAACATCCGACCGCTAAGGAGGCGATGGATGCGAAAAAACGCTATGAGGACCCTGCCAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATGCTGAGTGCCACTACAAGGGATCTGCGTCACATTTCTCATAATTCATGTTTTTCTAATAATTAGAATATTAAACAATAACAATCCAGTACTGGAATCATTTGGAATCTTTACATTATGCCGTGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTcagc                                                                                                                                                                                     <  1:457344/289‑1 (MQ=255)
                                                                                                                                 ttcCTAAACCAGACTCGTTGATCAGTGAACATCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACAAGGGATCTGCGTCACATTTTTCATAATTCATGTTTTTCTAATAATTAGAATATTAAACAATAACAATCCATTACTGGAATCATTTGGAATCTTTACATTATGCCGTGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGCAGTGGTGCTTc                                                                                                                                                                          <  2:140865/300‑1 (MQ=255)
                                                                                                                                                    gATCAGTGAACATCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACAAGGGATCTGCGTCACATTTTTCATAATTCATGTTTTTCTAATAATTAGAATATTAAACAATAACAATCCATTACTGGAATCATTTGGAATCTTTACATTATGCCGTGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGCAGTGGTGCTTCACACTTGCCCGGTACTTat                                                                                                                                                       >  1:168295/1‑300 (MQ=255)
                                                                                                                                                    gATCAGTGAACATCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACAAGGGATCTGCGTCACATTTTTCATAATTCATGTTTTTCTAATAATTAGAATATTAAACAATAACAATCCATTACTGGAATCATTTGGAATCTTTACATTATGCCGTGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGCAGTGGTGCTTCACACTTGCCCGGTAATTaa                                                                                                                                                       >  2:78896/1‑301 (MQ=255)
                                                                                                                                                       cAGTGAACATCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACAAGGGATCTGCGTCACATTTTTCATAATTCATGTTTTTCTAATAATTAGAATATTAAACAATAACAATCCATTACTGGAATCATTTGGAATCTTTACATTATGCCGTGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGCAGTGGTGCTTCACACTTGCCCGGTAATTAacga                                                                                                                                                    >  2:27479/1‑301 (MQ=255)
                                                                                                                                                                                       atgCGAAAAAACGCGATGAGGACCCTGACAAAGGGTGACCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACAAGGGATCTGCGTCACATTTTTCATAATTCATGTTTTTCTAATAATTAGAATATTAAACAATAACAATCCATTACTGGAATCATTTGGAATCTTTACATTATGCCGTGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGCAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGt                                                                                                                    <  1:1938/301‑1 (MQ=255)
                                                                                                                                                                                        ggCGAAAAAAAGGTAAGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGGTTCTGAGTGCCACTACAAGGGATCTGCGTCACATTTTTCATAATTCATGTTTTTCTAATAATTAGAATATTAAACAATAACAATCCATTACTGGAATCATTTGGAATCTTTACATTATGCCGTGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGCAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTg                                                                                                                   <  1:2710/300‑1 (MQ=255)
                                                                                                                                                                                               aaaCGCGATGAGGACCCTTACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACAAGGGATCTGCGTCACATTTTTCATAATTCATGTTTTTCTAATAATTAGAATATTAAACAATAACAATCCATTACTGGAATCATTTGGAATCTTTACATTATGCCGTGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGCAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGtt                                                                                                            <  1:589878/301‑1 (MQ=255)
                                                                                                                                                                                                 aCGCTATTAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGTATTCTGAGTGCCACTACAAGGGATCTGCGTCACATTTTTCATAATTCATGTTTTTCTAATAATTAGAATATTAAACAATAACAATCCATTACTGGAATCATTTGGAATCTTTACATTATGCCGTGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGCAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTgg                                                                                                          <  2:39882/301‑1 (MQ=255)
                                                                                                                                                                                                  cgttATGAGGACCCTGACAAAGTGTGACCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACAAGGGATCTGCGTCACATTTTTCATAATTCATGTTTTTCTAATAATTAGAATATTAAACAATAACAATCCATTACTGGAATCATTTGGAATCTTTACATTATGCCGTGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGCAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTGGc                                                                                                         <  1:496925/298‑1 (MQ=255)
                                                                                                                                                                                                     tATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACAAGGGATCTGCGTCACATTTTTCATAATTCATGTTTTTCTAATAATTAGAATATTAAACAATAACAATCCATTACTGGAATCATTTGGAATCTTTACATTATGCCGTGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGCAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTGGCGAt                                                                                                      >  1:56063/1‑301 (MQ=255)
                                                                                                                                                                                                      agtcgtgtgctcttccgatctGACCGCATCAGACTGCTCGGAAGGGATTCTGAGTTCCACTACAAGGGATCTGCGTCACATTTTTCATAATTCATGTTTTTCTAATAATTAGAATATTAAACAATAACAATCCATTACTGGAATCATTTGGAATCTTTACATTATGCCGTGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGCAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTGGCGATc                                                                                                     <  2:147818/281‑1 (MQ=255)
                                                                                                                                                                                                        gAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACAAGGGATCTGCGTCACATTTTTCATAATTCATGTTTTTCTAATAATTAGAATATTAAACAATAACAATCCATTACTGGAATCATTTGGAATCTTTACATTATGCCGTGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGCAGTGGTTCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTGGCGATCCt                                                                                                   >  1:44971/1‑301 (MQ=255)
                                                                                                                                                                                                         aGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACAAGGGATCTGCGTCACATTTTTCATAATTCATGTTTTTCTAATAATTAGAATATTAAACAATAACAATCCATTACTGGAATCATTTGGAATCTTTACATTATGCCGTGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGCAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTGGCGATCCTg                                                                                                  <  2:168492/301‑1 (MQ=255)
                                                                                                                                                                                                           gACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACAAGGGATCTGCGTCACATTTTTCATAATTCATGTTTTTCTAATAATTAGAATATTAAACAATAACAATCCATTACTGGAATCATTTGGAATCTTTACATTATGCCGTGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGCAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTGGCGATCCTGAc                                                                                                <  2:215152/301‑1 (MQ=255)
                                                                                                                                                                                                             cccTGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACAAGGGATCTGCGTCACATTTTTCATAATTCATGTTTTTCTAATAATTAGAATATTAAACAATAACAATCCATTACTGGAATCATTTGGAATCTTTACATTATGCCGTGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCCCAAGTAAGTGTCAGCAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGACAGAAAAGTAAGGTGGATGAACAATGAGTGAGTCGTTGGCAATCCTGACCa                                                                                              >  1:473371/1‑301 (MQ=255)
                                                                                                                                                                                                               cTGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACAAGGGATCTGCGTCACATTTTTCATAATTCATGTTTTTCTAATAATTAGAATATTAAACAATAACAATCCATTACTGGAATCATTTGGAATCTTTACATTATGCCGTGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGCAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTGGCGATCCTGACCATc                                                                                            >  2:630400/1‑301 (MQ=255)
                                                                                                                                                                                                                     cgatttGACCGGATCAGACTGCTTGGAAGGGATTCTGAGTGCCACTACAAGGGATCTGCGTCACATTTTTCATAATTCATGTTTTTCTAATAATTAGAATATTAAACAATAACAATCCATTACTGGAATCATTTGGAATCTTTACATTATGCCGTGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGCAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTGGCGATCCTGACCATCGGCAtt                                                                                      <  1:567171/296‑1 (MQ=255)
                                                                                                                                                                                                                           gACCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACAAGGGATCTGCGTCACATTTTTCATAATTCATGTTTTTCTAATAATTAGAATATTAAACAATAACAATCCATTACTGGAATCATTTGGAATCTTTACATTATGCCGTGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGCAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTGGCGATcagatcggaagagcgtcgggta                                                                                >  1:147692/1‑280 (MQ=255)
                                                                                                                                                                                                                           gACCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACAAGGGATCTGCGTCACATTTTTCATAATTCATGTTTTTCTAATAATTAGAATATTAAACAATAACAATCCATTACTGGAATCATTTGGAATCTTTACATTATGCCGTGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGCAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTGGCGATCCTGACCATCGGCAttagatcg                                                                                >  2:567885/1‑295 (MQ=255)
                                                                                                                                                                                                                                cATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACAAGGGATCTGCGTCACATTTTTCATAATTCATGTTTTTCTAATAATTAGAATATTAAACAATAACAATCCATTACTGGAATCATTTGGAATCTTTACATTATGCCGTGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGCAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTGGCGATCCTGACCATCGGCATTGTACCTATGCa                                                                           <  2:526700/301‑1 (MQ=255)
                                                                                                                                                                                                                                          cTCGGAAGGGATTCTGAGTGCCACTACAAGGGATCTGCGTCACATTTTTCATAATTCATGTTTTTCTAATAATTAGAATATTAAACAATAACAATCCATTACTGGAATCATTTGGAATCTTTACATTATGCCGTGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGCAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTGGCGATCCTGACCATCGGCATTGTACCTATGCAGGAAGttttt                                                                 >  1:537021/1‑300 (MQ=255)
                                                                                                                                                                                                                                          cTCGGAAGGGATTCTGAGTGCCACTACAAGGGATCTGCGTCACATTTTTCATAATTCATGTTTTGCTAATAATTAGAATATTAAACAATAACAATCCATTACTGGAATCATTTGGAATCTTTACATTATGCCGTGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGCAGTGGTGCTTCACACTTACCCGGTAATTAACGACGAAAGCACAGTAAGGTGGAGGAACACTGAGTGCGACGTTGGCGATCCTGACCAACGGCATGGTACACAGGAAGGCAGttttt                                                                 >  1:526355/1‑300 (MQ=255)
                                                                                                                                                                                                                                          cTCGGAAGGGATTCTGAGTGCCACTACAAGGGATCTGCGTCACATTTTTCATAATTCATGTTTTGCTAATAATTAGAATATTAAACAATAACAATCCATTACTGGAATCATTTGGAATCTTTACATTATGCCGTGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGCAGTGGTGCTTCACACTTACCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTGGCGATCCTGACCATCGGCATTGTACCTATGCAGGAAGttttt                                                                 >  1:526341/1‑300 (MQ=255)
                                                                                                                                                                                                                                            gggAAGGGATTCTGAGTGCCACTACAAGGGATCTGCGTCACATTTTTCATAATTCATGTTTTTCTAATAATTAGAATATTAAACAATAACAATCCATTACTGGAATCATTTGGAATCTTTACATTATGCCGTGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGCAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTGGCGATCCTGACCATCGGCATTGTACCTATTCAGGAAGTTTTGcc                                                               <  1:78806/300‑1 (MQ=255)
                                                                                                                                                                                                                                                    aTTCTGAGTGCCACTACAAGGGATCTGCGTCACATTTTTCATAATTCATGTTTTTCTAATAATTAGAATATTAAACAATAACAATCCATTACTGGAATCATTTGGAATCTTTACATTATGCCGTGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGCAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTGGCGATCCTGACCATCGGCATTGTCCCTATGCAGGAAGTTTTACCGCTCCTGa                                                       >  2:183144/1‑301 (MQ=255)
                                                                                                                                                                                                                                                        tGAGTGCCACCACAAGGGATCTGCGTCACATTTTTCATAATTCATGTTTTTCTAATAATTAGAATATTAAACAATAACAATCCATTACTGGAATCATTTGGAATCTTTACATTATGCCGTGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGCAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTGGCGATCCTGACCATCGGCATTGTACCTATGCAGGAAGTTTTGCCGCTCCTGACGGa                                                   >  2:623794/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                    cAAGGGATCTGCGTCACATTTTTCATAATTCATGTTTTTCTAATAATTAGAATATTAAACAATAACAATCCATTACTGGAATCATTTGGAATCTTTACATTATGCCGTGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGCAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTGGCGATCCTGACCATCGGCAATGTACCTATGCAAGAAGTTTTGCCGCTCCTGACGGAATCAATTGACGa                                       >  1:364826/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                          aTCTGCGTCACATTTTTCATAATTCATGTTTTTCTAATAATTAGAATATTAAACAATAACAATCCATTACTGGAATCATTTGGAATCTTTACATTATGCCGTGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGCAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTGGCGATCCTGACCATCGGCATTGTACCTATGCAGGAAGTTTTGCCGCTCCTGACGGAATACATTGACGAAAataa                                 >  2:437843/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                           tgtGCGTCACAGTTTTCAGAATTCATGGTTTTGTAAGGATTGTAATATTAAACAATAACGATCCGTGACGTGAATAATTGGGAAGCGTTACATTAAGCCGTGCACGTCTGCTGCTACGCTTTTTGTCAGTGGTAGCACAAGTAAGGGTCAGCAGTGGTGCTTCACACTTGCCCGGTAATGAACGACGAAAGAAAAGTAAGGGGGATGAACAATGAGTGCGTCGTTGGCGATCCTGACCAGCGGCATTGTACCTATGCAGGAAGGTTTGCCGCTCCTGACGGAATACATTGACGAAGataat                                <  1:536607/299‑1 (MQ=255)
                                                                                                                                                                                                                                                                           tCTGCGTCACATTTTTCATAATTCATGTTTTTCTAATAATTAGAATATTAAACAATAACAATCAATGACTGGAATCATTTTGAATCTTTACATTATGCCGTGCACGTCTGCTGGTACGCTTTTTGTGATGTGTAGCACAAGTAAGTGTCAGCAGTGGTGATTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTGGCGATCCTGACCATCGGCATTGTACCTATGCAGGAAGTTTTGCCGCTCCTGACGGAATACATTGACGAAGataat                                <  1:536595/301‑1 (MQ=255)
                                                                                                                                                                                                                                                                                 tCACATTTTTCATAATTCATGTTTTTCTAATAATTAGAATATTAAACAATAACAATCCATTACTGGAATCATTTGGAATCTTTACATTATGCCGTGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGCAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTGGCGATCCTGACCATCGGCATTGTACCTATGCAGGAAGTTTTGCCGCTCCTGACGGAATACATTGACGAAGATAATATTTcc                          >  2:534823/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                                 tCACATTTTTCATAATTCATGTTTTTCTAATAATTAGAATATTAAACAATAACAATCCATTACTGGAATCATTTGGAATCTTTACATTATGCCGTGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGCAGCGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTGGCGATCCTGACCATCGGCATTGTACCTATGCAGGAAGTTTTGCCGCTCCTGACGGAATACATTGACGAAGATAATATTTcc                          >  2:559852/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                                   tcgatcttCATAATTCATGTTTTTCTAATAATTAGAATATTAAACAATAACAATCCATTACTGGAATCATTTGGAATCTTTACATTATGCCGTGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGCAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTGGCGATCCTGACCATCGGCATTGTACCTATGCAGGAAGTTTTGCCGCTCCTGACGGAATACATTGACGAAGATAATATTTCCca                        <  2:193912/295‑1 (MQ=255)
                                                                                                                                                                                                                                                                                   tcaTTTTTCATATTTCATGTTTTGCTAATAATTAGAATATTAAACAATAACAATCCATTACTGGAATCATTTGGAATCTTTACATTATGCCGTGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGCAGTGGTGCTTCACACTTACCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTGGCGATCCTGACCATCGGCATTGTACCTATGCAGGAAGTTTTGCCGCTCCTGACGGAATACATTGACGAAGATAATATTTCCca                        <  2:527013/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                                   ataTTTTTCATAATTCATGTTTTGCTAATAATTAGAATATTAAACAATAACAATCCATTACTGGAATCATTTGGAATCTTTACATTATGCCGTGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGCAGTGGTGCTTCACACTTACCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTGGCGATCCTGACCATCGGCATTGGACCTATGCAGGAAGTTTTGCCGCTCCTGACGGAATACATTGACGAAGATAATATTTcaca                        <  2:527027/299‑1 (MQ=255)
                                                                                                                                                                                                                                                                                   acaTTTTTCATAATTCATGTTTTTCTAATAATTAGAATATTAAACAATAACAATCCATTACTGTAATCATTTGGAATCTTTACATTATGCCGTGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGCAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTGGCGATCCTGACCATCGGCATTGTACCTATGCAGGAAGTTTTGCCGCTCCTGACGGAATACATTGACGAAGATAATATTTCCca                        <  1:27428/301‑1 (MQ=255)
                                                                                                                                                                                                                                                                                    caTTTTTCATAATTCATGTTTTTCTAATAATTAGAATATTAAACAATAACAATCCATTACTGGAATCATTTGGAATCTTTACATTATGCCGTGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGCAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTGGCGATCCTGACAATCGGCATTGTACCTATGCAAGAAGTTTTGCCGCTCCTGACGGAATACATTGACGAAGATAATATTTCCcat                       >  1:58051/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                                          tCATAATTCATGTTTTTCTAATAATTAGAATATTAAACAATAACAATCCATTACTGGAATCATTTGGAATCTTTACATTATGCCGTGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGCAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTGGCGATCCTGACCATCGGCATTGTACCTATGCAGGAAGTTTTGCCGCTCCTGACGGAATACATTGACGAAGAAAATATTTCCcaagatcgg                 >  1:193669/1‑294 (MQ=255)
                                                                                                                                                                                                                                                                                                  taTGTTTTTCTAATAATTAGAATATTAAACAATAACAATCCATTACTGGAATCATTTGGAATCTTTACATTATGCCGTGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGCAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTGGCGATCCTGACCATCGGCATTGTACCTATGCAGGAAGTTTTGCCGCTCCTGACGGAATACATTGACGAAGATAATATTTCCCATCATAGCCTGCTggg         <  2:473972/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                                                    tttttttCTAATAATTAGAATATTAAACAATAACAATCCATTACTGGAATCATTTGGAATCTTTACATTATGCCGTGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGCAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTGGCGATCCTGACCATCGGCATTGTACCTATGCAGGAAGTTTTGCCGCTCCTGACGGAATACATTGACGAAGATAATATTTCCCATCATAGCCTGCTGGGGa       <  1:629523/299‑1 (MQ=255)
                                                                                                                                                                                                                                                                                                         ttCTAATAATTAGAATATTAAACAATAACAATCCATTACTGGAATCATTTGGAATCTTTACATTATGCCGTGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGCAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTGGCGATCCTGACCATCGGCATTGTACCTATGCAGGAAGTTTTGCCGCTCCTGACGGAATACATTGACGAAGATAATATTTCCCATCATAGCCTGCTGGGGAAGTTa  <  2:365310/301‑1 (MQ=255)
                                                                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                                                           
ACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGATTGAAAAAGGAAAGCCAGGACAGACGGTAACCTGGTACCAACTCAGAGCCGATCATCCTAAACCAGACTCGTTGATCAGTGAACATCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTCTGAGTGCCACTACAAGGGATCTGCGTCACATTTTTCATAATTCATGTTTTTCTAATAATTAGAATATTAAACAATAACAATCCATTACTGGAATCATTTGGAATCTTTACATTATGCCGTGCACGTCTGCTGCTACGCTTTTTGTCATTTGTAGCACAAGTAAGTGTCAGCAGTGGTGCTTCACACTTGCCCGGTAATTAACGACGAAAGAAAAGTAAGGTGGATGAACAATGAGTGCGTCGTTGGCGATCCTGACCATCGGCATTGTACCTATGCAGGAAGTTTTGCCGCTCCTGACGGAATACATTGACGAAGATAATATTTCCCATCATAGCCTGCTGGGGAAGTTA  >  NC_000913/406950‑407547

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑