Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,009,118 T→G 6.4% intergenic (‑59/‑52) rhtB ← / → pldB homoserine, homoserine lactone and S‑methyl‑methionine efflux pump/lysophospholipase L2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,009,1180TG 6.4% 144.4 / 2.1 52intergenic (‑59/‑52)rhtB/pldBhomoserine, homoserine lactone and S‑methyl‑methionine efflux pump/lysophospholipase L2
Reads supporting (aligned to +/- strand):  ref base T (22/26);  new base G (2/2);  total (24/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.15e-01

CCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTAAAAAAAACGATTTTACGTGGTTTAAGAGGCAGATTACCCGATGTTTCAGCAGCAAAAAGACTGGGAAACAAGAGAAAACGCGTTTGCTGCTTTTACCATGGGACCGCTGACTGATTTCTGGCGTCAGCGTGATGAAGCAGAGTTTACTGGTGTGGATGACATTCCGGTGCGCTTTGTCCGTTTTCGCGCACAGCACCATGACCGGGTGGTAGTCATCTGCCCGGGGCGTATTGAGAGCTACGTAAAATATGCGGAACTGGCCTATGACCTGTTCCATTTGGG  >  NC_000913/4008819‑4009411
                                                                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                                                                     
ccGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTc                                                                                                                                                                                                                                                                                                      <  2:490928/301‑1 (MQ=255)
  gcgcCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCTAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGAAGCGATGTGTCAg                                                                                                                                                                                                                                                                                                    >  2:553023/1‑301 (MQ=255)
                caacacttcaaACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACCATCCGCTACTGTCGCAACGTTGTTTCAGGCCCGCTaaaaaaa                                                                                                                                                                                                                                                                                      >  1:610517/1‑301 (MQ=255)
                  acacttcaaCCGCAATAACTGGGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCACAGCGTTGTGTCAGGCACGCTAAAAAAAAc                                                                                                                                                                                                                                                                                    <  1:58114/301‑1 (MQ=255)
                              aaaTCAAGGAGCGGGAAACAAGCGTCCCCAGCCCCACGCCAACCAGCACATTTTGATTCGCCAGTCCGGTCTTAAGCCCAGCAATAGGCGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTAAAAAAAACGATTTTACGTgg                                                                                                                                                                                                                                                                        <  1:15083/300‑1 (MQ=255)
                                  cattGAGCGTGAAAATAGCGTCCACAACCCCATGCCAACCAGCACAATATTAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTAAAAAAAACGATTTTACGTGGTTTa                                                                                                                                                                                                                                                                    <  2:383282/298‑1 (MQ=255)
                                                  aGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCACAGCGTTGTGTCAGGCACGCTAAAAAAAACGATTTTACGTGGTTTAAGAGGCAGATTACCCg                                                                                                                                                                                                                                                    <  2:57084/301‑1 (MQ=255)
                                                                         cAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTAAAAAAAACGATTTTACGTGGTTTAAGAGGCAGATTACCCGATGTTTCAGCAGCAAAAAGACTg                                                                                                                                                                                                                             <  2:611375/301‑1 (MQ=255)
                                                                          aGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTAAAAAAAACGATTTTACGTGGTTTAAGAGGCAGATTACCCGATGTATCAGCAGCAAAAAGACTgg                                                                                                                                                                                                                            >  2:235448/1‑301 (MQ=255)
                                                                           gCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTAAAAAAAACGATTTTACGTGGTTTAAGAGGCAGATTACCCGATGTTTCAGCAGCAAAAAGACTggg                                                                                                                                                                                                                           >  2:608848/1‑301 (MQ=255)
                                                                              caATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGCGTGTCTATTCTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTAAAAAAACCGAATTTACGGGGTTTAAGAGTCAGATTACACGATGTTTACGCAGCaaaacgccagagaaa                                                                                                                                                                                                                        >  1:173734/1‑290 (MQ=255)
                                                                                 aattgatcgcagggtcGGGCTGATGTCCAGCAATGGACTCCACCGCTCTGCGCTAACGTTGGGTGAGCGGGGTGGTCATAGTGTTGATTGCCCCAGAGCCGTGCGACAGGCTTAAAATGTTCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTAAAAAAAACGATTTTACGTGGTTTAAGAGGCAGATTACCCGATGTTTCAGCAGCAAAAAGACTGGGAAACaa                                                                                                                                                                                                                     <  1:401910/286‑1 (MQ=255)
                                                                                      aTCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTAAAAAAAACGATTTTACGTGGTTTAAGAGGCAGATTACCCGATGTTTCAGCAGCAAAAAGACTGGGAAACAAGAGaa                                                                                                                                                                                                                >  2:36408/1‑301 (MQ=255)
                                                                                                      ggagcccgtgaatgggtgtgaCCGGGCCGGGTTAACCGTGGTGGAGGGAGGGGGGTATAGTGTTGGTTGTACCAGAGCCTGGGGAAAGGCTTAAAATGGTCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGGCGCAGCGTTGTGTCAGGCACGCTAAAAAAAACGATTTTACGTGGTTTAAGAGGCAGATTACCCGATGTTTCAGCAGCAAGAAGACTGGGAAACAAGAGAAAACGCGTTTGCTGCtt                                                                                                                                                                                                <  1:282914/281‑1 (MQ=255)
                                                                                                           ccAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTAAAAAAAACGATTTTACGAGGTTTAAGAGGACGATTACCAGATGTTTAAGAAGCAAAAAGACTGGGAAACAAGAGAAAACACGGTTGCTGCTTTTAca                                                                                                                                                                                           >  2:523175/1‑300 (MQ=255)
                                                                                                            cAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTAAAAAAAACGATTTTACGTGGTTTAAGAGGCAGATTACCCGATGTTTCAGCAGCAAAAAGACTGGGAAACAAGAGAAAACGCGTTTGCTGCTTTTACCa                                                                                                                                                                                          >  2:104214/1‑301 (MQ=255)
                                                                                                                   aGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTAAAAAAAACGATTTTACGTGGTTTAAGAGGCAGATTACCCGATGTTTCAGCAGCAAAAAGACTGGGAAACAAGAGAAAACGCGTTTGCTGCTTTTACCATGGGAcc                                                                                                                                                                                   >  2:122546/1‑301 (MQ=255)
                                                                                                                                  gcggtgtccGTGGTTTAGTGGGGTGTTCATTGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGGGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTAAAAAAAACGATTTTACGTGGTTTAAGAGGCAGATTACCCGATGTTTCAGCAGCAAAAAGACTGGGAAACAAGAGAAAACGCGTTTGCTGCTTTTACCATGGGACCGCTGACTGATTTCTg                                                                                                                                                                    <  1:552350/294‑1 (MQ=255)
                                                                                                                                         ccGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTAAAAAAAACGATTTTACGTGGTTTAAGAGGCAGATTACCCGATGTTTCAGCAGCAAAAAGACTGGGAAACAAGAACAAACGCGTTTGCAGCTTTTACAATGGGACCGCTGACTGATTTCTGGCGTCAg                                                                                                                                                             >  1:217552/1‑301 (MQ=255)
                                                                                                                                                tGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTAAAAAAAACGATTTTACGTGGTTTAAGAGGCAGATTACCCGATGTTTCAGCAGCAAAAAGACTGGGAAACAAGAGAAAACGCGTTTGCTGCTTTTACCATGGGACCGCTGACTGATTTCTGGCGTCAGCGtgata                                                                                                                                                      >  2:409725/1‑300 (MQ=255)
                                                                                                                                                   gcgggggtgtgaTAGTGTTGATTGCACCAGGGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTAAAAAAAACGATTTTACGTGGTTTAAGAGGCAGATTACCCGATGTTTCAGCAGCAAAAAGACTGGGAAACAAGAGAAAACGCGTTTGCTGCTTTTACCATGGGGCCGCTGACTGATTTCTGGCGTCAGCGTGATGAAg                                                                                                                                                   <  1:607990/290‑1 (MQ=255)
                                                                                                                                                        gtggtCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTAAAAAAAACGATTTTACGTGGTTTAAGAGGCAGATTACCCGATGTTTCAGCAGCAAAAAGACTGGGAAACAAGAGAAAACGCGTTTGCTGCTTTTACCATGGGACCGCTGACTGATTTCTGGCGTCAGCGTGATGAAGCagag                                                                                                                                              <  2:506228/301‑1 (MQ=255)
                                                                                                                                                             cATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTAAAAAAAACGATTTTACGTGGTTTAAGAGGCAGATTACCCGATGTTTCAGCAGCAAAAAGACTGGGAAACAAGAGAAAACGCGTTTGCTGCTTTTACCATGGGACCGCTGACTGATTTCAGGCGTCAGCGTGATGAAGCAGAGTTTat                                                                                                                                         >  1:304526/1‑300 (MQ=255)
                                                                                                                                                                    ccttccgatttagggCCTGGCGGCAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGGCAGGCACGCTAAAAAAAACGATTTTACGTGGTTTAAGAGGCAGATTACCCGATGTTTCAGCAGCAAAAAGACTGGGAAACAAGAGAAAACGCGTTTGCTGCTTTTACCATGGGACCGCTGACTGATTTCTGGCGTCAGCGTGATGAAGCAGAGTTTACTGGtgtg                                                                                                                                  <  1:386725/287‑1 (MQ=255)
                                                                                                                                                                      cgatcattttaagCCTGTCGCCAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTAAAAAAAACGATTTTACGTGGTTTAAGAGGCAGATTACCCGATGTTTCAGCAGCAAAAAGACTGGGAAACAAGAGAAAACGCGTTTGCTGCTTTTACCATGGGACCGCTGACTGATTTCTGGCGTCAGCGTGATGAAGCAGAGTTTACTGGTGTGGa                                                                                                                                >  2:162804/12‑301 (MQ=255)
                                                                                                                                                                               agagCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTAAAAAAAACGATTTTACGTGGTTTAAGAGGCAGATTACCCGATGTTTCAGCAGCAAAAAGACTGGGAAACAAGAGAAAACGCGTTTGCTGCTTTTACCATGGGACCGCTGACTGATTTCTGGCGTCAGCGTGATGAAGCAGAGTTTACTGGtgtgagatcggaaga                                                                                                                       >  2:387239/1‑290 (MQ=255)
                                                                                                                                                                                   cagggCGACAGGCTTAAAATGTTGGATGTCAGCAGGTTGGCTAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAAGACGCTACTGTCGCAGCGTGGTGTCAGGCACGCTAAAAAAAACGATTTTACGTGGTTTAAGAGGCAGATTACCCGATGTTTCAGCAGCAAAAAGACTGGGAAACAAGAGAAAACGCGTTTGCTGCTGTTACCATGGGACCGCTGACTGATTTCTGGCGTCAGCGTGATGAAGCAGAGTTTACTGGTGTGGATGACATTCCGGTg                                                                                                                   <  1:36357/298‑1 (MQ=255)
                                                                                                                                                                                                 aaattggatcggggtgtgtagGTAGGGAAACGAAGGTGCTAAGGGCATGATGAACTCCCGGTTTGTCTATGTTTGGGGCACAATAGGCTGCTGTCGGAGCGTTGTGTCAGGCACGCGAAAGAAAACGATTTTACGTGGTTTAAGAGGCAGATTACCCGATGTTTCAGCAGCAAAAAGACTGGGAAACAAGAGAAAACGCGTTTGCTGCTGTTACCATGGGACCGCTGACTGATTTCTGGCGGCAGCGTGAGGAAGCAGAGTTTACTGGTGTGGATGACATTCCGGTGCGCTTTGTCCgatg                                                                                                     <  1:134028/282‑4 (MQ=255)
                                                                                                                                                                                                  aaaaTGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTAAAAAAAACGATTTTACGTGGTTTAAGAGGCAGATTACCCGATGTTTCAGCAGCAAAAAGACTGGGAAACAAGAGAAAACGCGTTTGCTGCTTTTACCATGGGACCGCTGACTGATTTCTGGCGTCAGCGTGATGAAGCAGAGTTTACTGGTGTGGATGACATTCCGGTGCGCTTTGTCCGtttt                                                                                                    <  2:304930/301‑1 (MQ=255)
                                                                                                                                                                                                   aaaTGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTAAAAAAAACGATTTTACGTGGTTTAAGAGGCAGATTACCCGATGTTTCAGCAGCAAAAAGACTGGGAAACAAGAGAAAACGCGTTTGCTGCTTTTACCATGGGACCGCTGACTGATTTCTGGCGTCAGCGTGATGAAGCAGAGTTTACTGGTGTGGATGACATTCCGGTACGCTTTGTCCGttttt                                                                                                   >  1:304976/1‑300 (MQ=255)
                                                                                                                                                                                                        aTCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCAACAATACGCTACGGTAGCAGCGTTGTGTCAGGCACGCTAAAAAAAACGATTTTACGTGGTTTAAGAGGCAGATTACCCGATGTTTCAGCAGCAAAAAGCCTGGGAACCAAGAGAAAACGCGTTTGCTGCTTTTACCATGGGACCGCTGACTGATTTCTGGCGTCAGCGAGATGAAGCAGCGTTTACTGGTGTGGATAACATTCCGGTGCGCGTTGTCCGATTTTGCGca                                                                                              >  1:381257/1‑301 (MQ=255)
                                                                                                                                                                                                           gATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTAAAAAAAACGATTTTACGTGGTTTAAGAGGCAGATTACCCGATGTTTCAGCAGCAAAAAGACTGGGAAACAAGAGAAAACGCGTTTGCTGCTTTTACCATGGGACCGCTGACTGATTTCTGGCGTCAGCGTGATGAAGCAGAGTTTACTGGTGTGGATGACATTCCGGTGCGCTTTGTCCGTTTTCGCGCACAg                                                                                           <  2:381072/301‑1 (MQ=255)
                                                                                                                                                                                                                cagcagGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTAAAAAAAACGATTTTACGTGGTTTAAGAGGCAGATTACCCGATGTTTCAGCAGCAAAAAGACTGGGAAACAAGAGAAAACGCGTTTGCTGCTTTTACCATGGGACCGCTGACTGATTTCTGGCGTCAGCGTGATGAAGCAGAGTTTACTGGTGTGGATGACATTCCGGTGCGCTTTGTCCGTTTTCGCGCCAAGCACCa                                                                                      >  1:275844/1‑301 (MQ=255)
                                                                                                                                                                                                                cagcagGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTAAAAAAAACGATTTTACGTGGTTTAAGAGGCAGATTACCCGATGTTTCAGCAGCAAAAAGACTGGGAAACAAGAGAAAACGCGTTTGCTGCTATTACCATGGGACCGCTGACTGATTTCTGGCGTCAGCGTGATGAAGCAGAGTTTACTGGTGTGGATGACATTCCGGTGCGCTTTGTCCGTTTTAGCGCACAGAACCa                                                                                      >  2:554868/1‑301 (MQ=255)
                                                                                                                                                                                                                            aagcgtccgttttAAGGTCATGGTGAACTTCCGTTGTGTCTGTTTTTGTGGCACTATACGCTACTGGCGCAGTGTTTTGTAAAGGACGCTAAAAAATACGATTTTACGTGGTTTAAGAGGCAGATTACCGTATGTTTCAGCAGCAAAAAGACTGGGAAACAAGAGAAAACGCGTTTGCTGCTTTTACCATGGGACCTCTGACTGATTTCTGGCGTCAGCGTGATGAAGCAGAGTTTACTGGTGTGGATGACATTCCGGTGCGCTTTGTCCGTTTTCGCGCACAGCACCATGACCGggtggt                                                                          <  1:285937/289‑1 (MQ=255)
                                                                                                                                                                                                                            aaaCCACCCTTCTAAGGTCATTATTAACTCCCGGCTTGTCTTTTTTTGTGCCACAATACGCTACTGGCGCAGCGTTGTGTCAGGGACGCTAAAAAAAACGATTTTACGTGGTTTAAGAGGCAGATTACCCGATGTGTCAGCAGCAAAAAGACTGGGAAACAAGAGAAAACGCGTTTGCTGCTTTTACCAGGGGACCGCTGACTGATTTCTGGCGTGAGCGTGATGAAGCAGAGTTTACTGGTGTGGATGACATTCCGGTGCGCTTTGTCCGTTTTCGCGCACAGCACCATGACCGggtggt                                                                          <  2:173931/301‑1 (MQ=255)
                                                                                                                                                                                                                              accaccATTTTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTAAAAAAAACGATTTTACGTGGTTTAAGAGGCAGATTACCCGATGTTTCAGCAGCAAAAAGACTGGGAAACAAGAGAAAACGCGTTTGCTGCTTTTACCATGGGACCGCTGACTGATTTCTGGCGTCAGCGTGATGAAGCAGAGTTTACTGGTGTGGATGACATTCCGGTGCGCTTTGTCCGTTTTCGCGCACAGCACCATGACCGGGTGGTAg                                                                        <  1:409162/301‑1 (MQ=255)
                                                                                                                                                                                                                                           ggtcagtgtgaACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTAAAAAAAACGATTTTACGTGGTTTAAGAGGCAGATTACCCGATGTTTCAGCAGCAAAAAGACTGGGAAACAAGAGAAAACGCGTTTGCTGCTTTTACCATGGGACCGCTGACTGATTTCTGGCGTCAGCGTGATGAAGCAGAGTTTACTGGTGTGGATGACATTCCGGTGCGCTTTGTCCGTTTTCGCGCACAGCACCATGACCGGGTGGTAGTCATCTGCCCggg                                                           <  1:235182/293‑1 (MQ=255)
                                                                                                                                                                                                                                             tCATGTTGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTAAAAAAAACGATTTTACGTGGTTTAAGAGGCAGATTACCCGATGTTTCAGCAGCAAAAAGACTGGGAAACAAGAGAAAACGCGTTTGCTGCTTTTACCATGGGACCGCTGACTGATTTCTGGCGTCAGCGTGATGAAGCAGAGTTTACTGGTGTGGATGACATTCCGGTGCGCTTTGTCCGTTTTCGCGCACAGCACCATGACCGGGTGGTAGTCATCTGCCCGGGGc                                                         <  1:162652/301‑1 (MQ=255)
                                                                                                                                                                                                                                                   tgtaCTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTAAAAAAAACGATTTTACGTGGTTTAAGAGGCAGATTACCCGATGTTTCAGCAGCAAAAAGACTGGGAAACAAGAGAAAACGCGTTTGCTGCTTTTACCATGGGACCGCTGACTGATTTCTGGCGTCAGCGTGATGAAGCAGAGTTTACTGGTGTGGATGACATTCCGGTGCGCTTTGTCCGTTTTCGCGCACAGCACCATGACCGGGTGGTAGTCATCTGCCCGGGGCGTATTg                                                   <  2:381753/298‑1 (MQ=255)
                                                                                                                                                                                                                                                          agttcagacgtgtgctcttccgatcttACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTAAAAAAAACGATTTTACGTGGTTTAAGAGGCAGATTACCCGATGTTTCAGCAGCAAAAAGACTGGGAAACAAGAGAAAACGCGTTTGCTGCTTTTACCATGGGACCGCTGACTGATTTCTGGCGTCAGCGTGATGAAGCAGAGTTTACTGGTGTGGATGACATTCCGGTGCGCTTTGTCCGTTTTCGCGCACAGCACCATGACCGGGTGGTAGTCATCTGCCCGGGGCGTATTGAGAGCTa                                            <  2:405781/275‑1 (MQ=255)
                                                                                                                                                                                                                                                                  tCTATTTTTGTGCCACAATACGCTACTGTTGCAGCGTTGTGTCAGGCACGCTAAAAAAAACGATTTTACGTGGTTTAAGAGGCAGATTACCCGATGTTTCAGCAGCAAAAAGACTGGGAAACAAGAGAAAACGCGTTTGCTGCTTTTACCATGGGACCGCTGACTGATTTCTGGCGTCAGCGTGATGAAGCAGAGTTTACTGGTGTGGATGACATTCCGGTGCGCTTTGTCCGTTTTCGCGCACAGCACCATGACCGGGTGGTAGTCATCTGCCCGGGGCGTATTGAGAGCTACGTAAAat                                    <  2:305380/301‑1 (MQ=255)
                                                                                                                                                                                                                                                                     ttttttGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTAAAAAAAACGATTTTACGTGGTTTAAGAGGCAGATTACCCGATGTTTCAGCAGCAAAAAGACTGGGAAACAAGAGAAAACGCGTTTGCTGCTTTTACCATGGGACCGCTGACTGATTTCTGGCGTCAGCGTGATGAAGCAGAGTTTACTGGTGTGGATGACATTCCGGTGCGCTTTGTCCGTTTTCGCGCACAGCACCATGACCGGGTGGTAGTCATCTGCCCGGGGCGTATTGAGAGCTACGTAAAATATg                                 <  2:217795/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                          cctgacacaacgctgcgacagtagcgtattGTGGCAGGCACGCTAAAAAAAACGATTTTACGTGGTTTAAGAGGCAGATTACCCGATGTTTCAGCAGCAAAAAGACTGGGAAACAAGAGAAAACGCGTTTGCTGCTTTTACCATGGGACCGCTGACTGATTTCTGGCGTCAGCGTGATGAAGCAGAGTTTACTGGTGTGGATGACATTCCGGTGCGCTTTGTCCGTTTTCGCGCACAGCACCATGACCGGGTGGTAGTCATCTGCCCGGGGCGTATTGAGAGCTACGTAAAATATGCGGaa                            >  1:291214/29‑301 (MQ=255)
                                                                                                                                                                                                                                                                           ctgacacaacgctgcgacagtagcgtattGTGGCAGGCACGCTAAAAAAAACGATTTTACGTGGTTTAAGAGGCAGATTACCCGATGTTTCAGCAGCAAAAAGACTGGGAAACAAGAGAAAACGCGTTTGCTGCTTTTACCATGGGACCGCTGACTGATTTCTGGCGTCAGCGTGATGAAGCAGAGTTTACTGGTGTGGATGACATTCCGGTTCGCTTTGTCCGTTTTCGCGCACAGCACCATGACCGGGTGGTAGTCATCTGCCCGGGGCGTATTGAGAGCTACGTAAAATATGCGGaaa                           >  2:36187/28‑300 (MQ=255)
                                                                                                                                                                                                                                                                            tgCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTAAAAAAAACGATTTTACGTGGTTTAAGAGGCAGATTACCCGATGTTTCAGCAGCAAAAAGACTGGGAAACAAGAGAAAACGCGTTTGCTGCTTTTACCATGGGACCGCTGACTGATTTCTGGCGTCAGCGTGATGAAGCAGAGTTTACTGGTGTGGATGACATTCCGGTGCGCTTTGTCCGTTTTCGCGCACAGCACCATGACCGGGTGGTAGTCATCTGCCCGGGGCGTATTGAGAGCTACGTAAAATATGCGGAACt                          >  2:225474/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                                   acgctgcgacagtagcgtattGTGGCAGGCACGCTAAAAAAAACGATTTTACGTGGTTTAAGAGGCAGATTACCCGATGTTTCAGCAGCAAAAAGACTGGGAAACAAGAGAAAACGCGTTTGCTGCTTTTACCATGGGACCGCTGACTGATTTCTGGCGTCAGCGTGATGAAGCAGAGTTTACTGGTGTGGATGACATTCCGGTGCGCTTTGTCCGTTTTCGCGCACAGCACCATGACCGGGTGGTAGTCATCTGCCCGGGGCGTATTGAGAGCTACGTAAAATATGCGGAACTGGCCTAt                   <  2:291618/282‑1 (MQ=255)
                                                                                                                                                                                                                                                                                    tACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTAAAAAAAACGATTTTACGTGGTTTAAGAGGCAGATTACCCGATGTTTCAGCAGCAAAAAGACTGGGAAACAAGAGAAAACGCGTTTGCTGCTTTTACCATGGGACCGCTGACTGATTTCTGGCGTCAGCGTGATGAAGCAGAGTTTACTGGTGTGGATGACATTCCGGTGCGCTTTGTCCGTTTTCGCGCACAGCACCATGACCGGGTGGTAGTCATCTGCCCGGGGCGTATTGAGAGCTaagatcggaagagcgtcgtgtagggaa                  >  1:405218/1‑275 (MQ=255)
                                                                                                                                                                                                                                                                                        cTACTGTCGCAGCGTTGTGTCAGGCACGCTAAAAAAAACGATTTTACGTGGTTTAAGAGGCAGATTACCCGATGTTTCAGCAGCAAAAAGACTGGGAAACAAGAGAAAACGCGTTTGCTGCTTTTACCATGGGACCGCTGACTGATTTCTGGCGTCAGCGTGATGAAGCAGAGTTTACTGGTGTGGATGACATTCCGGTGCGCTTTGTCCGTTTTCGCGCACAGCACCATGACCGGGTGGTAGTCATCTGCCCGGGGCGTATTGAGAGCTACGTAAAATATGCGGAACTGGCCTATGACCt              >  2:132592/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                                            ggttgcaggtttGTGTCAGGAACGCTAAAAAAAACGATTTTACGTGGTTTAAGAGGCAGATTACCCGATGTTTCAGCAGCAAAAAGACTGGGAAACAAGAGAAAACGCGGTTGCTGCTTGTACCATGGGACCGCTGACTGATTTCTGGCGTCAGCGTGATGAAGCAGAGTTTACTGGTGTGGATGACATTCCGGTGCGCTTTGTCCGTTTTCGCGCACAGCACCATGACCGGGTGGTAGTCATCTGCCCGGGGCGTATTGAGAGCTACGTAAAATATGCGGAACTGGCCTATGACCTGTTc          <  1:122422/291‑1 (MQ=255)
                                                                                                                                                                                                                                                                                             gTCGCAGCGTTGTGTCAGGCACGCTAAAAAAAACGATTTTACGTGGTTTAAGAGGCAGATTACCCGATGTTTCAGCAGCAAAAAGACTGGGAAACAAGAGAAAACGCGTTTGCTGCTTTTACCATGGGACCGCTGACTGATTTCTGGCGTCAGCGTGATGAAGCAGAGTTTACTGGTGTGGATGACATTCCGGTGCGCTTTGTCCGTTTTCGCGCACAGCACCATGACCGGGTGGTAGTCATCTGCCCGGGGCGTATTGAGAGCTACGTAAAATATGCGGAACTGGCCTATGACCTGCTcc         >  1:481273/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                                                    ggTTGTGTCAGGCACGCTAAAAAAAACGATTTTACGTGGTTTAAGAGGCAGATTACCCGATGTTTCAGCAGCAAAAAGACTGGGAAACAAGAGAAAACGCGTTTGCTGCTTTTACCATGGGACCGCTGACTGATTTCTGGCGTCAGCGTGATGAAGCAGAGTTTACTGGTGTGGATGACATTCCGGTGCGCTTTGTCCGTTTTCGCGCACAGCACCATGACCGGGTGGTAGTCATCTGCCCGGGGCGTATTGAGAGCTACGTAAAATATGCGGAACTGGCCTATGACCTGTTCCATTTggg  <  1:132467/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                                                                     
CCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTAAAAAAAACGATTTTACGTGGTTTAAGAGGCAGATTACCCGATGTTTCAGCAGCAAAAAGACTGGGAAACAAGAGAAAACGCGTTTGCTGCTTTTACCATGGGACCGCTGACTGATTTCTGGCGTCAGCGTGATGAAGCAGAGTTTACTGGTGTGGATGACATTCCGGTGCGCTTTGTCCGTTTTCGCGCACAGCACCATGACCGGGTGGTAGTCATCTGCCCGGGGCGTATTGAGAGCTACGTAAAATATGCGGAACTGGCCTATGACCTGTTCCATTTGGG  >  NC_000913/4008819‑4009411

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: