Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 4,009,118 | T→G | 6.4% | intergenic (‑59/‑52) | rhtB ← / → pldB | homoserine, homoserine lactone and S‑methyl‑methionine efflux pump/lysophospholipase L2 |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 4,009,118 | 0 | T | G | 6.4% | 144.4 / 2.1 | 52 | intergenic (‑59/‑52) | rhtB/pldB | homoserine, homoserine lactone and S‑methyl‑methionine efflux pump/lysophospholipase L2 |
Reads supporting (aligned to +/- strand): ref base T (22/26); new base G (2/2); total (24/28) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.15e-01 |
CCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTAAAAAAAACGATTTTACGTGGTTTAAGAGGCAGATTACCCGATGTTTCAGCAGCAAAAAGACTGGGAAACAAGAGAAAACGCGTTTGCTGCTTTTACCATGGGACCGCTGACTGATTTCTGGCGTCAGCGTGATGAAGCAGAGTTTACTGGTGTGGATGACATTCCGGTGCGCTTTGTCCGTTTTCGCGCACAGCACCATGACCGGGTGGTAGTCATCTGCCCGGGGCGTATTGAGAGCTACGTAAAATATGCGGAACTGGCCTATGACCTGTTCCATTTGGG > NC_000913/4008819‑4009411 | ccGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTc < 2:490928/301‑1 (MQ=255) gcgcCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCTAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGAAGCGATGTGTCAg > 2:553023/1‑301 (MQ=255) caacacttcaaACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACCATCCGCTACTGTCGCAACGTTGTTTCAGGCCCGCTaaaaaaa > 1:610517/1‑301 (MQ=255) acacttcaaCCGCAATAACTGGGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCACAGCGTTGTGTCAGGCACGCTAAAAAAAAc < 1:58114/301‑1 (MQ=255) aaaTCAAGGAGCGGGAAACAAGCGTCCCCAGCCCCACGCCAACCAGCACATTTTGATTCGCCAGTCCGGTCTTAAGCCCAGCAATAGGCGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTAAAAAAAACGATTTTACGTgg < 1:15083/300‑1 (MQ=255) cattGAGCGTGAAAATAGCGTCCACAACCCCATGCCAACCAGCACAATATTAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTAAAAAAAACGATTTTACGTGGTTTa < 2:383282/298‑1 (MQ=255) aGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCACAGCGTTGTGTCAGGCACGCTAAAAAAAACGATTTTACGTGGTTTAAGAGGCAGATTACCCg < 2:57084/301‑1 (MQ=255) cAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTAAAAAAAACGATTTTACGTGGTTTAAGAGGCAGATTACCCGATGTTTCAGCAGCAAAAAGACTg < 2:611375/301‑1 (MQ=255) aGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTAAAAAAAACGATTTTACGTGGTTTAAGAGGCAGATTACCCGATGTATCAGCAGCAAAAAGACTgg > 2:235448/1‑301 (MQ=255) gCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTAAAAAAAACGATTTTACGTGGTTTAAGAGGCAGATTACCCGATGTTTCAGCAGCAAAAAGACTggg > 2:608848/1‑301 (MQ=255) caATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGCGTGTCTATTCTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTAAAAAAACCGAATTTACGGGGTTTAAGAGTCAGATTACACGATGTTTACGCAGCaaaacgccagagaaa > 1:173734/1‑290 (MQ=255) aattgatcgcagggtcGGGCTGATGTCCAGCAATGGACTCCACCGCTCTGCGCTAACGTTGGGTGAGCGGGGTGGTCATAGTGTTGATTGCCCCAGAGCCGTGCGACAGGCTTAAAATGTTCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTAAAAAAAACGATTTTACGTGGTTTAAGAGGCAGATTACCCGATGTTTCAGCAGCAAAAAGACTGGGAAACaa < 1:401910/286‑1 (MQ=255) aTCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTAAAAAAAACGATTTTACGTGGTTTAAGAGGCAGATTACCCGATGTTTCAGCAGCAAAAAGACTGGGAAACAAGAGaa > 2:36408/1‑301 (MQ=255) ggagcccgtgaatgggtgtgaCCGGGCCGGGTTAACCGTGGTGGAGGGAGGGGGGTATAGTGTTGGTTGTACCAGAGCCTGGGGAAAGGCTTAAAATGGTCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGGCGCAGCGTTGTGTCAGGCACGCTAAAAAAAACGATTTTACGTGGTTTAAGAGGCAGATTACCCGATGTTTCAGCAGCAAGAAGACTGGGAAACAAGAGAAAACGCGTTTGCTGCtt < 1:282914/281‑1 (MQ=255) ccAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTAAAAAAAACGATTTTACGAGGTTTAAGAGGACGATTACCAGATGTTTAAGAAGCAAAAAGACTGGGAAACAAGAGAAAACACGGTTGCTGCTTTTAca > 2:523175/1‑300 (MQ=255) cAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTAAAAAAAACGATTTTACGTGGTTTAAGAGGCAGATTACCCGATGTTTCAGCAGCAAAAAGACTGGGAAACAAGAGAAAACGCGTTTGCTGCTTTTACCa > 2:104214/1‑301 (MQ=255) aGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTAAAAAAAACGATTTTACGTGGTTTAAGAGGCAGATTACCCGATGTTTCAGCAGCAAAAAGACTGGGAAACAAGAGAAAACGCGTTTGCTGCTTTTACCATGGGAcc > 2:122546/1‑301 (MQ=255) gcggtgtccGTGGTTTAGTGGGGTGTTCATTGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGGGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTAAAAAAAACGATTTTACGTGGTTTAAGAGGCAGATTACCCGATGTTTCAGCAGCAAAAAGACTGGGAAACAAGAGAAAACGCGTTTGCTGCTTTTACCATGGGACCGCTGACTGATTTCTg < 1:552350/294‑1 (MQ=255) ccGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTAAAAAAAACGATTTTACGTGGTTTAAGAGGCAGATTACCCGATGTTTCAGCAGCAAAAAGACTGGGAAACAAGAACAAACGCGTTTGCAGCTTTTACAATGGGACCGCTGACTGATTTCTGGCGTCAg > 1:217552/1‑301 (MQ=255) tGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTAAAAAAAACGATTTTACGTGGTTTAAGAGGCAGATTACCCGATGTTTCAGCAGCAAAAAGACTGGGAAACAAGAGAAAACGCGTTTGCTGCTTTTACCATGGGACCGCTGACTGATTTCTGGCGTCAGCGtgata > 2:409725/1‑300 (MQ=255) gcgggggtgtgaTAGTGTTGATTGCACCAGGGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTAAAAAAAACGATTTTACGTGGTTTAAGAGGCAGATTACCCGATGTTTCAGCAGCAAAAAGACTGGGAAACAAGAGAAAACGCGTTTGCTGCTTTTACCATGGGGCCGCTGACTGATTTCTGGCGTCAGCGTGATGAAg < 1:607990/290‑1 (MQ=255) gtggtCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTAAAAAAAACGATTTTACGTGGTTTAAGAGGCAGATTACCCGATGTTTCAGCAGCAAAAAGACTGGGAAACAAGAGAAAACGCGTTTGCTGCTTTTACCATGGGACCGCTGACTGATTTCTGGCGTCAGCGTGATGAAGCagag < 2:506228/301‑1 (MQ=255) cATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTAAAAAAAACGATTTTACGTGGTTTAAGAGGCAGATTACCCGATGTTTCAGCAGCAAAAAGACTGGGAAACAAGAGAAAACGCGTTTGCTGCTTTTACCATGGGACCGCTGACTGATTTCAGGCGTCAGCGTGATGAAGCAGAGTTTat > 1:304526/1‑300 (MQ=255) ccttccgatttagggCCTGGCGGCAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGGCAGGCACGCTAAAAAAAACGATTTTACGTGGTTTAAGAGGCAGATTACCCGATGTTTCAGCAGCAAAAAGACTGGGAAACAAGAGAAAACGCGTTTGCTGCTTTTACCATGGGACCGCTGACTGATTTCTGGCGTCAGCGTGATGAAGCAGAGTTTACTGGtgtg < 1:386725/287‑1 (MQ=255) cgatcattttaagCCTGTCGCCAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTAAAAAAAACGATTTTACGTGGTTTAAGAGGCAGATTACCCGATGTTTCAGCAGCAAAAAGACTGGGAAACAAGAGAAAACGCGTTTGCTGCTTTTACCATGGGACCGCTGACTGATTTCTGGCGTCAGCGTGATGAAGCAGAGTTTACTGGTGTGGa > 2:162804/12‑301 (MQ=255) agagCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTAAAAAAAACGATTTTACGTGGTTTAAGAGGCAGATTACCCGATGTTTCAGCAGCAAAAAGACTGGGAAACAAGAGAAAACGCGTTTGCTGCTTTTACCATGGGACCGCTGACTGATTTCTGGCGTCAGCGTGATGAAGCAGAGTTTACTGGtgtgagatcggaaga > 2:387239/1‑290 (MQ=255) cagggCGACAGGCTTAAAATGTTGGATGTCAGCAGGTTGGCTAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAAGACGCTACTGTCGCAGCGTGGTGTCAGGCACGCTAAAAAAAACGATTTTACGTGGTTTAAGAGGCAGATTACCCGATGTTTCAGCAGCAAAAAGACTGGGAAACAAGAGAAAACGCGTTTGCTGCTGTTACCATGGGACCGCTGACTGATTTCTGGCGTCAGCGTGATGAAGCAGAGTTTACTGGTGTGGATGACATTCCGGTg < 1:36357/298‑1 (MQ=255) aaattggatcggggtgtgtagGTAGGGAAACGAAGGTGCTAAGGGCATGATGAACTCCCGGTTTGTCTATGTTTGGGGCACAATAGGCTGCTGTCGGAGCGTTGTGTCAGGCACGCGAAAGAAAACGATTTTACGTGGTTTAAGAGGCAGATTACCCGATGTTTCAGCAGCAAAAAGACTGGGAAACAAGAGAAAACGCGTTTGCTGCTGTTACCATGGGACCGCTGACTGATTTCTGGCGGCAGCGTGAGGAAGCAGAGTTTACTGGTGTGGATGACATTCCGGTGCGCTTTGTCCgatg < 1:134028/282‑4 (MQ=255) aaaaTGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTAAAAAAAACGATTTTACGTGGTTTAAGAGGCAGATTACCCGATGTTTCAGCAGCAAAAAGACTGGGAAACAAGAGAAAACGCGTTTGCTGCTTTTACCATGGGACCGCTGACTGATTTCTGGCGTCAGCGTGATGAAGCAGAGTTTACTGGTGTGGATGACATTCCGGTGCGCTTTGTCCGtttt < 2:304930/301‑1 (MQ=255) aaaTGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTAAAAAAAACGATTTTACGTGGTTTAAGAGGCAGATTACCCGATGTTTCAGCAGCAAAAAGACTGGGAAACAAGAGAAAACGCGTTTGCTGCTTTTACCATGGGACCGCTGACTGATTTCTGGCGTCAGCGTGATGAAGCAGAGTTTACTGGTGTGGATGACATTCCGGTACGCTTTGTCCGttttt > 1:304976/1‑300 (MQ=255) aTCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCAACAATACGCTACGGTAGCAGCGTTGTGTCAGGCACGCTAAAAAAAACGATTTTACGTGGTTTAAGAGGCAGATTACCCGATGTTTCAGCAGCAAAAAGCCTGGGAACCAAGAGAAAACGCGTTTGCTGCTTTTACCATGGGACCGCTGACTGATTTCTGGCGTCAGCGAGATGAAGCAGCGTTTACTGGTGTGGATAACATTCCGGTGCGCGTTGTCCGATTTTGCGca > 1:381257/1‑301 (MQ=255) gATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTAAAAAAAACGATTTTACGTGGTTTAAGAGGCAGATTACCCGATGTTTCAGCAGCAAAAAGACTGGGAAACAAGAGAAAACGCGTTTGCTGCTTTTACCATGGGACCGCTGACTGATTTCTGGCGTCAGCGTGATGAAGCAGAGTTTACTGGTGTGGATGACATTCCGGTGCGCTTTGTCCGTTTTCGCGCACAg < 2:381072/301‑1 (MQ=255) cagcagGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTAAAAAAAACGATTTTACGTGGTTTAAGAGGCAGATTACCCGATGTTTCAGCAGCAAAAAGACTGGGAAACAAGAGAAAACGCGTTTGCTGCTTTTACCATGGGACCGCTGACTGATTTCTGGCGTCAGCGTGATGAAGCAGAGTTTACTGGTGTGGATGACATTCCGGTGCGCTTTGTCCGTTTTCGCGCCAAGCACCa > 1:275844/1‑301 (MQ=255) cagcagGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTAAAAAAAACGATTTTACGTGGTTTAAGAGGCAGATTACCCGATGTTTCAGCAGCAAAAAGACTGGGAAACAAGAGAAAACGCGTTTGCTGCTATTACCATGGGACCGCTGACTGATTTCTGGCGTCAGCGTGATGAAGCAGAGTTTACTGGTGTGGATGACATTCCGGTGCGCTTTGTCCGTTTTAGCGCACAGAACCa > 2:554868/1‑301 (MQ=255) aagcgtccgttttAAGGTCATGGTGAACTTCCGTTGTGTCTGTTTTTGTGGCACTATACGCTACTGGCGCAGTGTTTTGTAAAGGACGCTAAAAAATACGATTTTACGTGGTTTAAGAGGCAGATTACCGTATGTTTCAGCAGCAAAAAGACTGGGAAACAAGAGAAAACGCGTTTGCTGCTTTTACCATGGGACCTCTGACTGATTTCTGGCGTCAGCGTGATGAAGCAGAGTTTACTGGTGTGGATGACATTCCGGTGCGCTTTGTCCGTTTTCGCGCACAGCACCATGACCGggtggt < 1:285937/289‑1 (MQ=255) aaaCCACCCTTCTAAGGTCATTATTAACTCCCGGCTTGTCTTTTTTTGTGCCACAATACGCTACTGGCGCAGCGTTGTGTCAGGGACGCTAAAAAAAACGATTTTACGTGGTTTAAGAGGCAGATTACCCGATGTGTCAGCAGCAAAAAGACTGGGAAACAAGAGAAAACGCGTTTGCTGCTTTTACCAGGGGACCGCTGACTGATTTCTGGCGTGAGCGTGATGAAGCAGAGTTTACTGGTGTGGATGACATTCCGGTGCGCTTTGTCCGTTTTCGCGCACAGCACCATGACCGggtggt < 2:173931/301‑1 (MQ=255) accaccATTTTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTAAAAAAAACGATTTTACGTGGTTTAAGAGGCAGATTACCCGATGTTTCAGCAGCAAAAAGACTGGGAAACAAGAGAAAACGCGTTTGCTGCTTTTACCATGGGACCGCTGACTGATTTCTGGCGTCAGCGTGATGAAGCAGAGTTTACTGGTGTGGATGACATTCCGGTGCGCTTTGTCCGTTTTCGCGCACAGCACCATGACCGGGTGGTAg < 1:409162/301‑1 (MQ=255) ggtcagtgtgaACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTAAAAAAAACGATTTTACGTGGTTTAAGAGGCAGATTACCCGATGTTTCAGCAGCAAAAAGACTGGGAAACAAGAGAAAACGCGTTTGCTGCTTTTACCATGGGACCGCTGACTGATTTCTGGCGTCAGCGTGATGAAGCAGAGTTTACTGGTGTGGATGACATTCCGGTGCGCTTTGTCCGTTTTCGCGCACAGCACCATGACCGGGTGGTAGTCATCTGCCCggg < 1:235182/293‑1 (MQ=255) tCATGTTGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTAAAAAAAACGATTTTACGTGGTTTAAGAGGCAGATTACCCGATGTTTCAGCAGCAAAAAGACTGGGAAACAAGAGAAAACGCGTTTGCTGCTTTTACCATGGGACCGCTGACTGATTTCTGGCGTCAGCGTGATGAAGCAGAGTTTACTGGTGTGGATGACATTCCGGTGCGCTTTGTCCGTTTTCGCGCACAGCACCATGACCGGGTGGTAGTCATCTGCCCGGGGc < 1:162652/301‑1 (MQ=255) tgtaCTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTAAAAAAAACGATTTTACGTGGTTTAAGAGGCAGATTACCCGATGTTTCAGCAGCAAAAAGACTGGGAAACAAGAGAAAACGCGTTTGCTGCTTTTACCATGGGACCGCTGACTGATTTCTGGCGTCAGCGTGATGAAGCAGAGTTTACTGGTGTGGATGACATTCCGGTGCGCTTTGTCCGTTTTCGCGCACAGCACCATGACCGGGTGGTAGTCATCTGCCCGGGGCGTATTg < 2:381753/298‑1 (MQ=255) agttcagacgtgtgctcttccgatcttACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTAAAAAAAACGATTTTACGTGGTTTAAGAGGCAGATTACCCGATGTTTCAGCAGCAAAAAGACTGGGAAACAAGAGAAAACGCGTTTGCTGCTTTTACCATGGGACCGCTGACTGATTTCTGGCGTCAGCGTGATGAAGCAGAGTTTACTGGTGTGGATGACATTCCGGTGCGCTTTGTCCGTTTTCGCGCACAGCACCATGACCGGGTGGTAGTCATCTGCCCGGGGCGTATTGAGAGCTa < 2:405781/275‑1 (MQ=255) tCTATTTTTGTGCCACAATACGCTACTGTTGCAGCGTTGTGTCAGGCACGCTAAAAAAAACGATTTTACGTGGTTTAAGAGGCAGATTACCCGATGTTTCAGCAGCAAAAAGACTGGGAAACAAGAGAAAACGCGTTTGCTGCTTTTACCATGGGACCGCTGACTGATTTCTGGCGTCAGCGTGATGAAGCAGAGTTTACTGGTGTGGATGACATTCCGGTGCGCTTTGTCCGTTTTCGCGCACAGCACCATGACCGGGTGGTAGTCATCTGCCCGGGGCGTATTGAGAGCTACGTAAAat < 2:305380/301‑1 (MQ=255) ttttttGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTAAAAAAAACGATTTTACGTGGTTTAAGAGGCAGATTACCCGATGTTTCAGCAGCAAAAAGACTGGGAAACAAGAGAAAACGCGTTTGCTGCTTTTACCATGGGACCGCTGACTGATTTCTGGCGTCAGCGTGATGAAGCAGAGTTTACTGGTGTGGATGACATTCCGGTGCGCTTTGTCCGTTTTCGCGCACAGCACCATGACCGGGTGGTAGTCATCTGCCCGGGGCGTATTGAGAGCTACGTAAAATATg < 2:217795/300‑1 (MQ=255) cctgacacaacgctgcgacagtagcgtattGTGGCAGGCACGCTAAAAAAAACGATTTTACGTGGTTTAAGAGGCAGATTACCCGATGTTTCAGCAGCAAAAAGACTGGGAAACAAGAGAAAACGCGTTTGCTGCTTTTACCATGGGACCGCTGACTGATTTCTGGCGTCAGCGTGATGAAGCAGAGTTTACTGGTGTGGATGACATTCCGGTGCGCTTTGTCCGTTTTCGCGCACAGCACCATGACCGGGTGGTAGTCATCTGCCCGGGGCGTATTGAGAGCTACGTAAAATATGCGGaa > 1:291214/29‑301 (MQ=255) ctgacacaacgctgcgacagtagcgtattGTGGCAGGCACGCTAAAAAAAACGATTTTACGTGGTTTAAGAGGCAGATTACCCGATGTTTCAGCAGCAAAAAGACTGGGAAACAAGAGAAAACGCGTTTGCTGCTTTTACCATGGGACCGCTGACTGATTTCTGGCGTCAGCGTGATGAAGCAGAGTTTACTGGTGTGGATGACATTCCGGTTCGCTTTGTCCGTTTTCGCGCACAGCACCATGACCGGGTGGTAGTCATCTGCCCGGGGCGTATTGAGAGCTACGTAAAATATGCGGaaa > 2:36187/28‑300 (MQ=255) tgCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTAAAAAAAACGATTTTACGTGGTTTAAGAGGCAGATTACCCGATGTTTCAGCAGCAAAAAGACTGGGAAACAAGAGAAAACGCGTTTGCTGCTTTTACCATGGGACCGCTGACTGATTTCTGGCGTCAGCGTGATGAAGCAGAGTTTACTGGTGTGGATGACATTCCGGTGCGCTTTGTCCGTTTTCGCGCACAGCACCATGACCGGGTGGTAGTCATCTGCCCGGGGCGTATTGAGAGCTACGTAAAATATGCGGAACt > 2:225474/1‑301 (MQ=255) acgctgcgacagtagcgtattGTGGCAGGCACGCTAAAAAAAACGATTTTACGTGGTTTAAGAGGCAGATTACCCGATGTTTCAGCAGCAAAAAGACTGGGAAACAAGAGAAAACGCGTTTGCTGCTTTTACCATGGGACCGCTGACTGATTTCTGGCGTCAGCGTGATGAAGCAGAGTTTACTGGTGTGGATGACATTCCGGTGCGCTTTGTCCGTTTTCGCGCACAGCACCATGACCGGGTGGTAGTCATCTGCCCGGGGCGTATTGAGAGCTACGTAAAATATGCGGAACTGGCCTAt < 2:291618/282‑1 (MQ=255) tACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTAAAAAAAACGATTTTACGTGGTTTAAGAGGCAGATTACCCGATGTTTCAGCAGCAAAAAGACTGGGAAACAAGAGAAAACGCGTTTGCTGCTTTTACCATGGGACCGCTGACTGATTTCTGGCGTCAGCGTGATGAAGCAGAGTTTACTGGTGTGGATGACATTCCGGTGCGCTTTGTCCGTTTTCGCGCACAGCACCATGACCGGGTGGTAGTCATCTGCCCGGGGCGTATTGAGAGCTaagatcggaagagcgtcgtgtagggaa > 1:405218/1‑275 (MQ=255) cTACTGTCGCAGCGTTGTGTCAGGCACGCTAAAAAAAACGATTTTACGTGGTTTAAGAGGCAGATTACCCGATGTTTCAGCAGCAAAAAGACTGGGAAACAAGAGAAAACGCGTTTGCTGCTTTTACCATGGGACCGCTGACTGATTTCTGGCGTCAGCGTGATGAAGCAGAGTTTACTGGTGTGGATGACATTCCGGTGCGCTTTGTCCGTTTTCGCGCACAGCACCATGACCGGGTGGTAGTCATCTGCCCGGGGCGTATTGAGAGCTACGTAAAATATGCGGAACTGGCCTATGACCt > 2:132592/1‑301 (MQ=255) ggttgcaggtttGTGTCAGGAACGCTAAAAAAAACGATTTTACGTGGTTTAAGAGGCAGATTACCCGATGTTTCAGCAGCAAAAAGACTGGGAAACAAGAGAAAACGCGGTTGCTGCTTGTACCATGGGACCGCTGACTGATTTCTGGCGTCAGCGTGATGAAGCAGAGTTTACTGGTGTGGATGACATTCCGGTGCGCTTTGTCCGTTTTCGCGCACAGCACCATGACCGGGTGGTAGTCATCTGCCCGGGGCGTATTGAGAGCTACGTAAAATATGCGGAACTGGCCTATGACCTGTTc < 1:122422/291‑1 (MQ=255) gTCGCAGCGTTGTGTCAGGCACGCTAAAAAAAACGATTTTACGTGGTTTAAGAGGCAGATTACCCGATGTTTCAGCAGCAAAAAGACTGGGAAACAAGAGAAAACGCGTTTGCTGCTTTTACCATGGGACCGCTGACTGATTTCTGGCGTCAGCGTGATGAAGCAGAGTTTACTGGTGTGGATGACATTCCGGTGCGCTTTGTCCGTTTTCGCGCACAGCACCATGACCGGGTGGTAGTCATCTGCCCGGGGCGTATTGAGAGCTACGTAAAATATGCGGAACTGGCCTATGACCTGCTcc > 1:481273/1‑301 (MQ=255) ggTTGTGTCAGGCACGCTAAAAAAAACGATTTTACGTGGTTTAAGAGGCAGATTACCCGATGTTTCAGCAGCAAAAAGACTGGGAAACAAGAGAAAACGCGTTTGCTGCTTTTACCATGGGACCGCTGACTGATTTCTGGCGTCAGCGTGATGAAGCAGAGTTTACTGGTGTGGATGACATTCCGGTGCGCTTTGTCCGTTTTCGCGCACAGCACCATGACCGGGTGGTAGTCATCTGCCCGGGGCGTATTGAGAGCTACGTAAAATATGCGGAACTGGCCTATGACCTGTTCCATTTggg < 1:132467/300‑1 (MQ=255) | CCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAGGTGGTCATAGTGTTGATTGCACCAGAGCCTGGCGACAGGCTTAAAATGATCGATGTCAGCAGGTAGGCAAACCACCATTCTAAGGTCATGATGAACTCCCGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTAAAAAAAACGATTTTACGTGGTTTAAGAGGCAGATTACCCGATGTTTCAGCAGCAAAAAGACTGGGAAACAAGAGAAAACGCGTTTGCTGCTTTTACCATGGGACCGCTGACTGATTTCTGGCGTCAGCGTGATGAAGCAGAGTTTACTGGTGTGGATGACATTCCGGTGCGCTTTGTCCGTTTTCGCGCACAGCACCATGACCGGGTGGTAGTCATCTGCCCGGGGCGTATTGAGAGCTACGTAAAATATGCGGAACTGGCCTATGACCTGTTCCATTTGGG > NC_000913/4008819‑4009411 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |