Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,246,561 T→G 6.4% intergenic (+39/‑91) exuT → / → exuR hexuronate transporter/hexuronate regulon transcriptional repressor; autorepressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,246,5610TG 6.4% 151.0 / 2.4 54intergenic (+39/‑91)exuT/exuRhexuronate transporter/hexuronate regulon transcriptional repressor; autorepressor
Reads supporting (aligned to +/- strand):  ref base T (24/26);  new base G (2/2);  total (26/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.91e-01

TTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTGCCGCTGACCTGAAAGAGCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAACGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGCCATCATCATGCTGGAAGTTGAAGGCTATGTCGAAGTGCGTAAAGG  >  NC_000913/3246263‑3246851
                                                                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                                                  
ttACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTtct                                                                                                                                                                                                                                                                                                  >  2:538630/1‑301 (MQ=255)
           tCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTGAGAAGGCGGCttt                                                                                                                                                                                                                                                                                       <  2:126901/301‑1 (MQ=255)
           tCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAACCCGCCTTCCAACGCGGCctt                                                                                                                                                                                                                                                                                       >  1:556311/1‑301 (MQ=255)
               ttgtgTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTtca                                                                                                                                                                                                                                                                                   <  2:47401/300‑1 (MQ=255)
                tgtgTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTtcat                                                                                                                                                                                                                                                                                  >  1:230582/1‑301 (MQ=255)
                ggggtTTGTTCGTAAGGAAGTGGCAACGGCAAACGGTTTAACCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGTGCTGGTGGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTGCGCAGTTCTGGCAGTGTGCGACCTGTTAGGTGCGCTGGTTATCTAGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTtcat                                                                                                                                                                                                                                                                                  <  1:412793/298‑1 (MQ=255)
                  tgtTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACCTTAACGTTTCGTGTAAGTATTAATTTCCCTTCAAAGCCCCCCTCCCACGCCCCCTTTtcctcc                                                                                                                                                                                                                                                                                >  1:423831/1‑300 (MQ=255)
                   gtTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTtcatccc                                                                                                                                                                                                                                                                               >  1:568855/1‑299 (MQ=255)
                           gTAACGAAGTGGCAACGGCAAACGGTTTAACCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCAATGCGAGTa                                                                                                                                                                                                                                                                       >  2:187167/1‑301 (MQ=255)
                             aaCGAAGTGGCAACGGCAAACGGTTTAACCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTaga                                                                                                                                                                                                                                                                     <  2:569569/301‑1 (MQ=255)
                                         aCGGCAAACGGTTTAACCGGGGTTTCCGCTTGGCTGGCAAGTACGCTGTGTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTGAGGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCa                                                                                                                                                                                                                                                         <  1:537957/301‑1 (MQ=255)
                                            ggaatcGGTTTATCCGGGTTGTCCGGATGGCTGGCAAGTACGCTGTTTGCGTGGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTTTAGCTCTACTCGCagtg                                                                                                                                                                                                                                                      <  1:62183/296‑4 (MQ=255)
                                              taaCTGTTTAACCTGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTTTAGCTCTACTCGCagtgat                                                                                                                                                                                                                                                    <  2:633760/300‑6 (MQ=255)
                                              aaaGGGTTTAATCGGGGTTGCCGGATGGTTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGAACTTGCTGACACCATCGGCTGCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCGGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTTTAGCTCTACTCGCagtgat                                                                                                                                                                                                                                                    <  1:457125/301‑6 (MQ=255)
                                               aaCGGTTTAACCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGATTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCCTTTTCAACACTGCAAGTTTAGCtctaatcgaagagata                                                                                                                                                                                                                                                   >  1:632883/1‑286 (MQ=255)
                                                              ttGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAg                                                                                                                                                                                                                                    <  2:230825/300‑1 (MQ=255)
                                                                gTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTc                                                                                                                                                                                                                                  >  2:147430/1‑301 (MQ=255)
                                                                        ggctggcAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTCAGGTGGCGCAGGCAACACATAACGATCCCGCACCGCAACATTCACGTTTCATGTAAGTTTTAGTTTCCCTTCACAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTCGAGCTAAACTCGCAAAAGTGGTATACCAAATATAGTCGGCCGTAt                                                                                                                                                                                                                          >  1:429637/1‑301 (MQ=255)
                                                                          ctggcAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACGTAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCGCTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCa                                                                                                                                                                                                                        >  2:621421/1‑301 (MQ=255)
                                                                          ctggcAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTAACa                                                                                                                                                                                                                        >  1:592582/1‑301 (MQ=255)
                                                                              cAAGTTCGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGcc                                                                                                                                                                                                                    <  2:27701/301‑1 (MQ=255)
                                                                                 gTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTAGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATTCCTgg                                                                                                                                                                                                                 >  1:198740/1‑301 (MQ=255)
                                                                                    cGCTGGTTGTGGTTGTTGTCGGTGCACTGGCTGACAGCATCGGCTTCAGCCCGCTGTTCGCTGTTCTGGCAGTGGTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGAGTGAGGTGGCGCAGGAAACACATAGCGAGCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACGGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAgc                                                                                                                                                                                                              <  1:186925/301‑1 (MQ=255)
                                                                                         tttGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCata                                                                                                                                                                                                         <  2:593343/301‑1 (MQ=255)
                                                                                         tttGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCata                                                                                                                                                                                                         <  2:184181/301‑1 (MQ=255)
                                                                                                                                 tcggcacgctgtgtgtagtgttGGCAGGGTTGGACCTTTTAGGTGCGCTGGTGATCTGGACCGGGTTGCAGAACAAACAGGCGATTGAGGTGGCGCAGGAAACACATAACGAGCCCGCACAGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTGc                                                                                                                                                                 <  1:144125/280‑1 (MQ=255)
                                                                                                                                                     gggggaGTGTGTGACTTGTTAGTTGTGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGTGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGAAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAAGATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACGGAACCACGCCGTTTGTATCAACAACTTGCCGCTGACCTGAAAGAGCGCa                                                                                                                                             <  1:608423/296‑1 (MQ=255)
                                                                                                                                                     cTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCCCTGCGAGTAGAGCTAAACTCGAAAAAGGGGTTAAACAATAAACACCAGCCGTATCATGCCTGGAGACAAAATGAAAATCACTAAACCACGCAGTTTGTATCAACAACTTACCGCTGACAAGaaaacacccc                                                                                                                                             >  1:630597/1‑294 (MQ=255)
                                                                                                                                                               ggggcgtgttgtgggggggggTTATCTGGGCCGTGTTGCAGAACAAACGTGCGAGTGTGGTGGCGGAGGAAACACGTAACGATCCCGAACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTGCCGCTGACCTGAAAGAGCGCATCGAACAggg                                                                                                                                   <  1:620563/282‑1 (MQ=255)
                                                                                                                                                                    cTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAAAAAATTGCCGCTGACCTGAAAGAGCGCATCGAACAGGGCGTCt                                                                                                                              >  2:335638/1‑301 (MQ=255)
                                                                                                                                                                              cgcTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTGCCGCTGACCTGAAAGAGCGCATCGAACAGGGCGTCTATCTggtggg                                                                                                                    >  2:638023/1‑301 (MQ=255)
                                                                                                                                                                                      aTCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTGCCGCTGACCTGAAAGAGCGCATCGAACAGGGCGTCTATCTGGTGGGTGATaaaa                                                                                                            >  2:193125/1‑300 (MQ=255)
                                                                                                                                                                                        gtgtacggtgtTGCAGAACAAACCGGCGATTGAGGTGGCGAAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCAGCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTGCCGCTGACCTGAAAGAGCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAActg                                                                                                          <  1:637146/294‑1 (MQ=255)
                                                                                                                                                                                          ggACCGTGTTGCAGAACAATCCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTGCCGCTGACCTGAAAGAGCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAActgcc                                                                                                        <  2:424394/301‑1 (MQ=255)
                                                                                                                                                                                           gACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTGCCGCTGACCTGAAAGAGCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAActgcct                                                                                                       <  2:557025/301‑1 (MQ=255)
                                                                                                                                                                                                   tGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTGCCGATGACCTGAAAGAGCACATCGAACAGGGCATCTATCTGGTGGGTGATAAACTGCCTGCAGAACg                                                                                               >  1:317916/1‑301 (MQ=255)
                                                                                                                                                                                                             aCCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCGTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTGCCGCTGACCTGAAAGAGCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAACGCTTTATTGcc                                                                                     <  2:430201/301‑1 (MQ=255)
                                                                                                                                                                                                               cGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTGCCGCTGACCTGAAAGAGCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAACGCTTTATTGCCGa                                                                                   <  2:198983/301‑1 (MQ=255)
                                                                                                                                                                                                                                             aCGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTGCCGCTGACCTGAAAGAGCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAACGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCg                                                     >  2:140849/1‑301 (MQ=255)
                                                                                                                                                                                                                                               gATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTGCCGCTGACCTGAAAGAGCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAACGCTTTATTGCCGAAGatcggaagagcagcaggtagggaaagagtg                                                   >  1:295491/1‑272 (MQ=255)
                                                                                                                                                                                                                                                   ccgcaccgcaACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCACAAGTGGTATAACAAATATAGTCTGCCGTATCCTGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTGCCGCTGACCTGAAAGAGCGCATCGAACAGGGCGTCTATCTGGCGGGTGATAAACTGCCTGCAGACCGCTTTATTGCCACTGAAAAGAACGTCAGCCGCACGGTAATTCGGGAAga                                               >  1:371330/1‑300 (MQ=255)
                                                                                                                                                                                                                                                        cgggatcATTATCGTTTCTTGTAAGTTTTAGTTTCCTTTCAAAGCCGCTTTGTCAGGGGGCTTTTTCATCACTGGGAGTAGAGCTAAACTCGCAAAATGGGTATAACAAATATAGTCTGCCGTATCTTGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACGTGCCGCTGACCTGAAAGAGCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAACGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAGGCcatca                                          <  1:140724/295‑1 (MQ=255)
                                                                                                                                                                                                                                                        ccgtaagctggtctgttCGGGAAAGTGTTTGTTTCCGTTCAACTCAGCTTGCTCGGGTGGTTTTGTCTTCACTGTCGGTTGAGCTAAACTCTCAAATGTGGTATAACATATATCGTCTGCCGTATCATGCCTGGAGCGCATATGTACATCACTGAACCACGCCGTTTGTATCAACAACTTGCCGCTGACCGGAAAGAGCGCATCGGACAGTGCGTCTATCTGGGGTGTGATAAACTGCCTGCAGAACGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGGAGTTCGTGAAGCcatca                                          <  1:335233/286‑1 (MQ=255)
                                                                                                                                                                                                                                                        ccgtaACATTTACGTTTTTTTTTATTTTTAGTTTCCCTTCAAAGCCGACTTCTTAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTGCCGCTGACCTGAAAGAGCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAACGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGCcatca                                          <  2:631474/301‑1 (MQ=255)
                                                                                                                                                                                                                                                           ttgcgggatcgttatgtgtAAGTTTTATTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTGCCGCTGACCTGAAAGAGCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAACGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGCcatcatca                                       <  2:207616/286‑1 (MQ=255)
                                                                                                                                                                                                                                                            aaCATTATCGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTGCCGCTGACCTGAAAGAGCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAACGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGCcatcatcat                                      <  1:192882/301‑1 (MQ=255)
                                                                                                                                                                                                                                                                         cGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTGCCGCTGACCTGAAAGAGCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAACGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGCCATCATCATGCTGGAAGTTGaa                         >  2:405385/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                           tgtAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCAGATGGAAATCACTGAACCACGCCGTTTGTAGCAACAACTTGCCGCTGACCTGCAAGAGCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAACGCTTTATTGCCGATGAAACGAACGTCAGCCGTACGGTAGTTCGTGAAGCCATCATCATGCTGAAAGTTAAAgg                       >  1:604500/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                            gtAAGTTTTATGTTCCCTTCAAGGCCGCCTTGTCAGGCTGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATACCAATTATGGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTGCCGCTGACCTGAAAGAGCGCATCGAACAGGGCGTCTAGCTGGTGGGTGATAAACTGCCTGCAGAACGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGCCATCATCATGCTGGAAGTTGAAGGc                      <  1:147304/301‑1 (MQ=255)
                                                                                                                                                                                                                                                                                 tttttctttCCCTTCTATGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTGCCGCTGACCTGAAAGAGCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAACGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGCCATCATCATGCTGGAAGTTGAAGGCTATGt                 <  2:378406/295‑1 (MQ=255)
                                                                                                                                                                                                                                                                                 ttttAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTGCCGCTGACCTGACAGAGCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAACGCTTTATTGCCGATGAAAAGAACGTCAGCCGAACGGTAGTTCGTGAAGACATCATCATGCTGGacgctaaactccagtt                 >  1:428437/1‑286 (MQ=255)
                                                                                                                                                                                                                                                                                           ccTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTGCCGCTGACCTGAAAGATCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAACGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGCCATCATCATGCTGGAAGTTGAAGGCTATGTCGAAGTGCgg       >  2:345020/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                                           ccTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTGCCGCTGACCTGAAAGAGCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAACGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGCAATCATCATGCTGGAAGTTGAAGGCTATGTCGAAGTGCgg       >  1:55386/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                                               aaaaGCCGCCTGAGAAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTGCCGCTGACCTGAAAGAGCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAACGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGCCATCATCATGCTGGAAGTTGAAGGCTATGTCGAAGTGCGTaaaa   >  2:607927/2‑300 (MQ=255)
                                                                                                                                                                                                                                                                                                aaaGCCGCCTGAGAAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTGCCGCTGACCTGAAAGAGCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAACGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGCCATCATCATTCTGGAAGTTGAAGGCTATGTCGAAGTGCGTAAAgg  >  1:135847/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                                                  
TTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTGCCGCTGACCTGAAAGAGCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAACGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGCCATCATCATGCTGGAAGTTGAAGGCTATGTCGAAGTGCGTAAAGG  >  NC_000913/3246263‑3246851

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑