| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,507,106 | A→G | 7.3% | G263G (GGT→GGC) | fryC ← | putative enzyme IIC component of PTS |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,507,106 | 0 | A | G | 7.3% | 159.0 / 3.4 | 60 | G263G (GGT→GGC) | fryC | putative enzyme IIC component of PTS |
| Reads supporting (aligned to +/- strand): ref base A (32/23); new base G (2/3); total (34/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.44e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.63e-01 | |||||||||||
GCACTGCGCCCAGCCAGACAGCGGCGGTTGAGCCGACAATAGCGCCGACCATATACGACGGAATGGCGGTGATGGGGCTTTCCAGCGCAAACGGAATCGCACCCTCACTGATCCCCATAAACGCCAGGAACATGGCGGTCTTACCCTGCGGATAAAGTTGAGCGTTGAACAGGCGTTTGCCGGTTAAACGACGGTCGATAATGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAAGGGGGTGATGACGTAGTACATCGCCAGCATGACAAAAATGGCGGAAAGAATCGGGAGCAAAAATGTGGTTTTGAACGCTAACAGGAAATCCGGTAACTGGATTTTCTGGTTCATCCACTTCACAAGGTAACCGGCAACGATAGAAATAATCAGCGCACC > NC_000913/2506808‑2507399 | gCACTGCGCCCAGCCAGACAGCGGCGGTTGAGCCGACAATAGCGCCGACCATATACGACGGAATGGCGGTGATGGGGCTTTCCAGCGCAAACGGAATCGCACCCTCACTGATCCCCATAAACGCCAGGAACATGGCGGTCTTACCCTGCGGATAAAGTTGAGCGTTGAACAGGCGTTTGCCGGTTAAACGACGGTCGATAATGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGAAAAAACCAACGGTGACCGGACAAACGAGGTCAGGGGTAACGCTGAAGGCAACAACAcc > 1:584930/1‑301 (MQ=255) cggaggcgggcgagtgctcggggtgtgtgCCGACGAGTGCGCCGACCATAGTGTGCGGTAGGGCGGTGATGGTGCTTGTCAGCGCAAACGGAATCGGCCGCTCACTGATCCCCATAAACGCCAGGAACATGGCGGTCTTACCCTGCGGAGAAAGTTTGGCGGTGAACAGGCGTTTGCCGGTTAAACGACGGTCGATAATGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGGGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGc < 1:149/273‑1 (MQ=255) gccagACAGCGGCGGTTGAGCCGACAATAGCGCCGACCATATACGACGGAATGGCGGTGATGGGGCTTTCCAGCGCAAACGGAATCGCACCCTCACTGATCCCCATAAACGCCAGGAACATGGCGGTCTTACCCTGCGGATAAAGTTGAGCGTTGAACAGGCGTTTGCCGGTTAAACGACGGTCGATAATGGCCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTttgtt > 2:159614/1‑301 (MQ=255) ccagACAGCGGCGGTTGAGCCGACAATAGCGCCGACCATATACGACGGAATGGCGGTGATGGGGCTTTCCAGCGCAAACGGAATCGCACCCTCACTGATCCCCATAAACGCCAGGAACATGGCGGTCTTACCCTGCGGATAAAGTTGAGCGTTGAACAGGCGTTTGCCGGTTAAACGACGGTCGATAATGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAGCCGCAGGTTttgttg < 2:396895/301‑1 (MQ=255) cagtccgcggcgGTTGAGCCGACAATAGCGCCGACCATATACGACGGAATTGCGGTGATGGGGCTTTCCAGCGCAAACGGAATCGCACCCTCACTGATCCCCATAAACGCCAGGAACATGGCGGTCTTACCCTGCGGATAAAGTTGAGCGTTGAACAGGCGTTTGCCGGTTAAACGACGGTCGATAATGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAGCCGCAGGTTttgttgc < 2:433758/295‑2 (MQ=255) ggcggTTGAGCCGACAATAGCGCCGACCATATACGACGGAATGGCGGTGATGGGGCTTTCCAGCGCAAACGGAATCGCACCCTCACTGATCCCCATAAACGCCAGGAACATGGCGGTCTTACCCTGCGGATAAAGTTGAGCGTTGAACAGGCGTTTGCCGGTTAAACGACGGTCGATAATGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGcca > 2:300101/1‑301 (MQ=255) aTAGCGCCGACCATATACGACGGAATGGCGGTGATGGGGCTTTCCAGCGCAAACGGAATCGCACCCTCACTGATCCCCATAAACGCCAGGAACATGGCGGTCTTACCCTGCGGATAAAGTTGAGCGTTGAACAGGCGTTTGCCGGTTAAACGACGGTCGATAATGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACATGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGcag > 2:267082/1‑299 (MQ=255) gcCGACCATATACGACGGAATGGCGGTGATGGGGCTTTCCAGCGCAAACGGAATCGCACCCTCACTGATCTCCATAAACGCCAGGAACATGGCGGTCTTACCCTGCGGATAACGTTGAGCGTTGAACAGGCGTTTGCCGGTTAAACGACGGTCGATAATGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGGGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCAATTGCTGTcgcc > 2:421861/1‑301 (MQ=255) tataCGACGGAATGGCGGTGATGGGGCTTTCCAGCGCAAACGGAATCGCACCCTCACTGATCCCCATAAACGCCAGGAACATGGCGGTCTTACCCTGCGGATAAAGTTGAGCGTTGAACAGGCGTTTGCCGGTTAAACGACGGTCGATAATGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGaa > 2:312872/1‑300 (MQ=255) ataCGACGGAATGGCGGTGATGGGGCTTTCCAGCGCAAACGGAATCGCACCCTCACTGATCCCCATAAACGCCAGGAACATGGCGGTCTTACCCTGCGGATAAAGTTGAGCGTTGAACAGGCGTTTGCCGGTTAAACGACGGTCGATAATGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCAGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGacc > 1:477564/1‑299 (MQ=255) acgacgGAATGGCGGTGATGGGGCTTTCCAGCGCAAACGGAATCGCACCCTCACTGATCCCCATAAACGCCAGGAACATGGCGGTCTTACCCTGCGGATAAAGTTGAGCGTTGAACAGGCGTTTGCCGGTTAAACGACGGTCGATAATGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGCACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGAGGATTTGTTGAACGGGCCACCAAGGTCGATAGCCATCGCCGCGACGATccc > 1:476546/1‑301 (MQ=255) gggAATGGCGGTGATGGGGCTTTCCAGCGCAAACGGAATCGCACCCTCACTGATCCCCATAAACGCCAGGAACATGGCGGTCTTACCCTGCGGATAAAGTTGAGCGTTGAACAGGCGTTTGCCGGTTAAACGACGGTCGATAATGGCCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCAtt < 1:159463/300‑1 (MQ=255) cgacgctcttccgatctaaTCGCACCCTCACTGATCCCCATAAACGCCAGGAACATGGCGGTCTTACCCTGCGGATAAAGTTGAGCGTTGAACAGGCGTTTGCCGGTTAAACGACGGTCGATAATGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCt < 1:85486/284‑1 (MQ=255) ttCCAGCGCAAACGGAATCGCACCCTCACTGATCCCCATAAACGCCAGGAACATGGCGGTCTTACCCTGCGGATAAAGTTGAGCGTTGAACAGGCGTTTGCCGGTTAAACGACGGTCGATAATGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTtc < 2:478165/301‑1 (MQ=255) cAGCGCAAACGGAATCGCACCCTCACTGATCCCCATAAACGCCAGGAACATGGCGGTCTTACCCTGCGGATAAAGTTGAGCGTTGAACAGGCGTTTGCCGGTTAAACGACGGTCGATAATGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTTCGTACATCAGCGCGCCCTTCTCa > 2:471250/1‑301 (MQ=255) cAGCGCAAACGGAATCGCACCCTCACTGATCCCCATAAACGCCAGGAACATGGCGGTCTTACCCTGCGGATAAAGTTGAGCGTTGAACAGGCGTTTGCCGGTTAAACGACGGTCGATAATGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCACCACGACTATCCCCATTGCGTACATAACCCCGCCCTtcccc > 1:377446/1‑298 (MQ=255) aCGGAATCGCACCCTCACTTATCCCCATAAACGCCAGGAACAGGGCGGTCTTACCCTGCGGATAAAGTTGAGCGTTGAACAGGCGTTGTCCGGTTAAACGACGGTCGATAATGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGc < 1:312468/301‑1 (MQ=255) aaTCGCACCCTCACTGATCCCCATAAACGCCAGGAACATGGCGGTCTTACCCTGCGGATAAAGTTGAGCGTTGAACAGGCGTTTGCCGGTTAAACGACGGTCGATAATGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCtagatcggaagagcacaa > 2:85610/1‑284 (MQ=255) ccTCACTGATCCCCATAAACGCCAGGAACATGGCGGTCTTACCCTGCGGATAAAGTTGAGCGTTGAACAGGCGTTTGCCGGTTAAACGACGGTCGATAATGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGt < 2:189898/301‑1 (MQ=255) ccTCACTGATCCCCATAAACGCCAGGAACATGGCGGTCTTACCCTGCGGATAAAGTTGAGCGTTGAACAGGCGTTTGCCGGTTAAACGACGGTCGATAATGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCAACAAGGTCGATTGCAGTCGCCGCGGCGATCCCCATCGCGTACAACCACCCGCCCTCCACAACTGCAGCACTCAGCCCAgc > 1:520244/1‑300 (MQ=255) ccTCAATGATCCCCTTATACGCCAGGAACATGGGGGTCGTACCCTGCGGATAAAGTTGAGCGTTGAACAGGCGTTTGCCGGTGAAACGACGGTCGATAATGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCAGTGAGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGt < 1:470649/301‑1 (MQ=255) ttGATCCCCATAAACGCCAGGAACATGGCGGTCTTACCCTGCGGATAAAGTTGAGCGTTGAACAGGCGTTTGCCGGTTAAACGACGGTCGATAATGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGa < 2:377942/300‑1 (MQ=255) tCCCCATAAACGCCAGGAACATGGCGGTCTTACCCTGCGGATAAAGTTGAGCGTTGAACAGGCGTTTGCCGGTTAAACGACGGTCGATAATGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATAcc < 2:477147/301‑1 (MQ=255) tACCCTGCGGATAAAGTTGAGCGTTGAACAGGCGTTTGCCGGTTAAACGACGGTCGATAATGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTTACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAAGGGGGtgat < 2:218198/301‑1 (MQ=255) tACCCTGCGGATAAAGTTGAGCGTTGAACAGGCGTTTGCCGGTTAAACGACGGTCGATAATGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCACCCTTCTCACCTGCGGCAGTCCGCCCAGTACGGATACCGCCCTTGACCCAACAACCAAAggggggaat > 1:516104/1‑297 (MQ=255) cTGCGGATAAAGTTGAGCGTTGAACAGGCGTTTGCCGGTTAAACGACGGTCGATAATGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCCTTGCGTACATCCGCGCCCCCTTCTCACCTTCGGCAGCCAGCACAGTACGGCAACCCCCGTTGAACCAGCCACCAAAGGGGATGCTGAcc > 1:372770/1‑300 (MQ=255) cGGATAAAGTTGAGCGTTGAACAGGCGTTTGCCGGTTAAACGACGGTCGATAATGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAAGGGGGTGATGACgtag < 2:585654/301‑1 (MQ=255) aaGTTGAGCGTTGAACAGGCGTTTGCCGGTTAAACGACGGTCGATAATGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATACAGCCGTTGAACCAGCCACCAAAGGGGGTGATGACGTAGTACATc > 1:116008/1‑301 (MQ=255) gAGCGTTGAACAGGCGTTTGCCGGTTAAACGACGGTCGATAATGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAAGGGGGTGATGACGTAGTACATCGCCAg < 2:83140/301‑1 (MQ=255) gTTGAACAGGCGTTTGCCGGTTAAACGACGGTCGATAATGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAAGGGGGTGATGACGAAGTAAATCGCCAGCAta > 2:423015/1‑300 (MQ=255) gAACAGGCGTTTGCCGGTTAAACGACGGTCGATAATGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAAGGGGGTGATGACGTAGTACATCGCCAGCATGACa > 2:455598/1‑301 (MQ=255) aaCAGGCGTTTGCCGGTTAAACGACGGTCGATAATGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCATCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAAGGGGGTGATGACGTAGTACATCGCCAGCATGACaa > 2:372356/1‑301 (MQ=255) ggCGTTTGCCGGTTAAACGACGGTCGTTAATGGTCGCCAGCGCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAAGGGGGTGATGACGTAGTACATCGCCAGCATGACAAAAAt < 1:299697/301‑1 (MQ=255) ggTTAAACGACGGTCGATAATGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAAGGGGGTGATGACGTAGTACATCGCCAGCATGACAAAAATGGCGGAAAGa > 2:21659/1‑301 (MQ=255) tAAACGACGGTCGATAATGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCCCCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCAACGGGGGTGATGACGAAGTAAATCGCCAACATCACAAACATAACGGAAAGaaca > 1:286693/1‑299 (MQ=255) ggTCGATAATGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAAGGGGGTGATGACGTAGTACATCGCCAGCATGACAAAAATGGCGGAAAGAATCGGGAGCaa > 2:393260/1‑301 (MQ=255) tAATGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAAGGGGGTGATGACGTAGTACATCGCCAGCATGACAAAAATGGCGGAAAGAAACGGGAGCAAAAAtgg > 2:264104/1‑300 (MQ=255) gtggccagccaGCGACCAATCGGCGGAATAACGATAGCGTTATAACGAGCGGTGGCCGGTAAGACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCGGCGGGTGTGTTGATCGGGCAACCAAGGGCGATTGCGGTCGCCGCGGCGATCCCCATTGCGGACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGAGACCGCCGTTGATCCAGCCACCAAAGGGGGTGATGACGTAGTACATCGCCAGCATGACAAAAATGGCGGAAAGAATCGGGAGCAAAAATGTGGttt < 1:421298/298‑1 (MQ=255) gTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAAGGGGGTGATGACGTAGTACATCGCCAGCATGACAAAAATGGCGGAAAGAATCGGGAGCAAAAATGTGGttt < 2:116132/301‑1 (MQ=255) ccccAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCGTTGGTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAAGGGGGTGATGACGTAGTACATCGCCAGCATGACAAAAATGGCGGAAAGAATCGGGAGCAAAAATGTGGTTTTGAACGCt < 1:371860/301‑1 (MQ=255) ccAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAAGGGGGTGATGACGTAGTACATCGCCAGCATGACAAAAATGGCGGAAAGAATCGGGAGCAAAAATGTGGTTTTGAACGCTAACAGGa > 2:255773/1‑301 (MQ=255) cAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAGGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGAGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAAGGGGGTGATGACGTAGTACATCGCCAGCATGACAAAAATGGAGGAAAGAATCGGGACAAAAAATATGGTTTTGAACTCTAACAGGaa > 1:547347/1‑301 (MQ=255) gcggAATAACTATAGCGATAGGACGATCGGTGTGCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCTGCGTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAAGGGGGTGATGACGTAGTACATCGCCAGCATGACAAAAATGGCGGAAAGAATCGGGAGCAAAAATGTGGTTTTGAACGCTAACAGGAAATCCgg < 1:422452/301‑1 (MQ=255) gcggAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAAGGGGGTGATGACGTAGTACATCGCCAGCATGACAAAAATGGCGGAAAGAATCGGGAGCAAAAATGTGGTTTTGAACGCTAACAGGAAATCCgg > 2:126622/1‑301 (MQ=255) gggaataacgggtgggttggaaggggCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAAGGGGGTGATGACGTAGTACATCGCCAGCATGACAAAAATGGCGGAAAGAATCGGGAGCAAAAATGTGGTTTTGAACGCTAACAGGAAATCCGGt < 1:455034/276‑1 (MQ=255) cgatagcgatagAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAAGGGGGTGATGACGTAGTACATCGCCAGCATGACAAAAATGGCGGAAAGAATCGGGAGCAAAAATGTGGTTTTGAACGCTAACAGGAAATCCGGTAACTGGaa > 2:511574/1‑300 (MQ=255) gatagAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAGGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAAGGGGGTGATGACGTAGTACATCGCCAGCATGACAAAAATGGCGGAAAGAATCGGGAGCAAAAATGTGGTTTTGAACGCTAACAGGAAATCCGGTAACTGGATTTTCTgg < 2:548020/301‑1 (MQ=255) ggggCGGTGACCGGTAATACGTGGTCGGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGAGTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAAGGGGGTGATGACGTAGTACATCGCCAGCATGACAAAAATGGCGGAAAGAATCGGGAGCAAAAATGTGGTTTTGAACGCTAACAGGAAATCCGGTAACTGGATTTTCTGGTTCATcc < 1:510902/298‑1 (MQ=255) aGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCACAGGGGGTGATGACGTAGTACATCGCCAGCATGACAAAAATGGCGGAAAGAATCGGGAGCAAAAATGTGGTTTTGAACGCTAACAGGAAATCCGGTAACTGGATTTTCTGGTTCATCCAc > 2:390878/1‑301 (MQ=255) aGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAAGGGGGTGATGACGTAGTACATCGCCAGCATGACAAAAATGGCGGAAAGAATCGGGAGCAAAAATGTGGTTTTGAACGCTAACAGGAAATCCGGTAACTGGATTTTCTGGTTCATCCaa > 2:74265/1‑300 (MQ=255) ggACCGGTAATGCGTGGTTCGTGGTAAAGCTGAAGGCAACAATACCTGCGGTTTTGTTGTTCGGGCCACCAAGGTCGATGGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAAGGGGGTGATGACGTAGTACATCGCCAGCATGACAAAAATGGCGGAAAGAATCGGGAGCAAAAATGTGGTTTTGAACGCTAACAGGAAATCCGGTAACTGGATTTTCTGGTTCATCCACTTcac < 1:392746/300‑1 (MQ=255) ggTGAGTGGTAAAGCTGATGGGACCCATGCGTGCGGCTTTGTTGATCGGGTCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCGGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAAGGGGGTGATGACGTAGTACATCGCCAGCATGACAAAAATGGCGGAAAGAATCGGGAGCAAAAATGTGGTTTTGAACGCTAACAGGAAATCCGGTAACTGGATTTTCTGGTTCATCCACTTCACAAGGTAGCCGGCAAc < 1:266814/301‑1 (MQ=255) ggTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAAGGGGGTGATGACGTAGTACATCGCCAGCATGACAAAAATGGCGGAAAGAATCGGGAGCAAAAATGTGGTTTTGAACGCTAACAGGAAATCCGGTAACTGGATTTTCTGGTTCATCCACTTCACAAGGTAACCGGCAACGATAGaa < 1:255505/301‑1 (MQ=255) aaaGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAAGGGGGTGATGACGTAGTACATCGCCAGCATGACAAAAATGGCGGAAAGAATCGGGAGCAAAAATGTGGTTTTGAACGCTAACAGGAAATCCGGTAACTGGATTTTCTGGTTCATCCACTTCACAAGGTAACCGGCAACGATAGAaata > 2:613083/1‑301 (MQ=255) cTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAAGGGGGTGATGACGTAGTACATCGCCAGCATGACAAAAATGGCGGAAAGAATCGGGAGCAAAAATGTGGTTTTGAACGCTAACAGGAAATCCGGTAACTGGATTTTCTGATTCATCCACTTCACAAGGTAACCGGCAACGATAGAAATAATCa > 1:497407/1‑301 (MQ=255) aGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCACAGGGGGTGATGACGTAGTACATCGCCAGCATGACAAAAATGGCGGAAAGAATCGGGAGCAAAAATGTGGTTTTGAACGCTAACAGGAAATCCGGTAACTGGATTTTCTGGTTCATCCACTTCACAAGGTAACCGGCACCGATAGAAATAAACAgcgc > 1:24459/1‑301 (MQ=255) gCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAAGGGGGTGATGACGTAGTACATCGCCAGCATGACAAAAATGGCGGAAAGAATCGGGAGCAAAAATGTGGTTTTGAACGCTAACAGGAAATCCGGTAACTGGATTTTCTGGTTCATCCACTTCACAAGGTAACCGGCAACGATAGAAATAATCAGCGCaa > 2:39270/1‑300 (MQ=255) caacaaAGCCGCAGGTTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAAGGGGGTGATGACGTAGTACATCGCCAGCATGACAAAAATGGCGGAAAGAATCGGGAGCAAAAATGTGGTTTTGAACGCTAACAGGCACTCCGGTAACTGGATTTTCTGGTTCATCCAATTCACAAGGTAACCGGCAACGACAGAAAAAATCAACGCAcc > 1:586282/1‑301 (MQ=255) caacaaAGCCGCAGGTTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAAGGGGGTGATGACGTAGTACATCGCCAGCATGACAAAAATGGCGGAAAGAATCGGGAGCAAAAATGTGGTTTTGAACGCTAACAGGAAATCCGGTAACTGGATTTTCTGGTTCATCCACTTCACAAGGTAACCGGCAACGATAGAAATAATCAGCGCAcc > 2:613385/1‑301 (MQ=255) aaccaaAACCGGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAAGGGGGTGATGACGTAGTACATCGCCAGCATGACAAAAATGGCGGAAAGAATCGGGAGCAAAAATGTGGTTTTGAACGCTAACAGGAAATCCGGTAACTGGATTTTCTGGTTCATCCACTTCACAAGGTAACCGGCAACGATAGAAATAATCAGCGCAcc < 1:74175/298‑1 (MQ=255) | GCACTGCGCCCAGCCAGACAGCGGCGGTTGAGCCGACAATAGCGCCGACCATATACGACGGAATGGCGGTGATGGGGCTTTCCAGCGCAAACGGAATCGCACCCTCACTGATCCCCATAAACGCCAGGAACATGGCGGTCTTACCCTGCGGATAAAGTTGAGCGTTGAACAGGCGTTTGCCGGTTAAACGACGGTCGATAATGGTCGCCAGCCCCAGACCAATCGGCGGAATAACGATAGCGATAGAACGAGCGGTGACCGGTAATACGTGGTCAGTGGTAAAGCTGAAGGCAACAAAACCTGCGGCTTTGTTGATCGGGCCACCAAGGTCGATTGCTGTCGCCGCGGCGATCCCCATTGCGTACATCAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAAGGGGGTGATGACGTAGTACATCGCCAGCATGACAAAAATGGCGGAAAGAATCGGGAGCAAAAATGTGGTTTTGAACGCTAACAGGAAATCCGGTAACTGGATTTTCTGGTTCATCCACTTCACAAGGTAACCGGCAACGATAGAAATAATCAGCGCACC > NC_000913/2506808‑2507399 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |