Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,419,544 A→T 7.9% L82Q (CTG→CAG)  yfcE ← phosphodiesterase activity on bis‑pNPP

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,419,5440AT 7.9% 205.3 / 2.4 70L82Q (CTG→CAG) yfcEphosphodiesterase activity on bis‑pNPP
Reads supporting (aligned to +/- strand):  ref base A (33/30);  new base T (2/4);  total (36/34)
Fisher's exact test for biased strand distribution p-value = 4.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTGTTGCCACGGCGCGGTTATCGGGAAATGCAGCAGCATTTGATCCACTTCGCTGTCGCAGTTGCCGCGCACAGCGATAACCTTATGTGCCACTTCATTAAGCCGTTCAGCGACTTTGGCTGGCGCGTAACCCTCCGGTAAAGCATTACGCGGGCCGTGATTCAACACGTCGCCAAGGATCACCAGCCACTGGGCACCGCTTTGGGCAAACAACTCCAGAACACGTTCCGTCGCCGGTAACGACCCATGAATGTCCGATGCAAACATCAGTTTCATCACTCACTCCTCGTCGAAAAAACGTAAGCTATGATACTGGATTCTGTCGTTGCTATCAG  >  NC_000913/2419253‑2419844
                                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                                                            
tGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGACAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTCTGACCGTACACCAGCACATCGTTCTGGTTTAACGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAACAGACGTTGTTTTTCCAGTAATACCTGTTGCCACGGCGCGGTTACCGGGAAATGCAGCAGCATTTGaac                                                                                                                                                                                                                                                                                                    >  1:62527/1‑299 (MQ=255)
tGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGACACTGGCAGATGGGTATGACCGTACACCAGCACCTCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCAAAAAAGCTGACCATGCGTCAAAAACAGAAGTTGTTTTTCCAGTAATACATGTTGCCACGGCGCGGTTAACGGGAAAGTCCGCGGCAATTGAt                                                                                                                                                                                                                                                                                                     >  1:69305/1‑301 (MQ=255)
  cgcAATGTTACTTTGTTCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTGTTGCCACGGCGCGGTTATCGGGAAATGCAGCAGCATTTGATcc                                                                                                                                                                                                                                                                                                   <  1:470317/301‑1 (MQ=255)
          ttcTTTGATCATTTGGTGCGGTTACACGAAGTACGTCATTATCCAGCATGCCATAACTTGGCGGATTACCGCCCTTCGGAATACTCACCGGGCCGGGGTTGAAGGGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTGTTGCCACGGCGCGGTTATCGGGAAATGCAGCAGCATTTGATCCACTTCGCt                                                                                                                                                                                                                                                                                           <  1:219032/299‑1 (MQ=255)
              tgctcttccgatctcGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTGTTGCCACGGCGCGGTTATCGGGAAATGCAGCAGCATTTGATCCACTTCGCTGTCg                                                                                                                                                                                                                                                                                       <  2:393758/287‑1 (MQ=255)
                 ttCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTGTTGCCACGGCGCGGTTATCGGGAAATGCAGCAGCATTTGATCCACTTCGCTGTCGCAg                                                                                                                                                                                                                                                                                    <  2:27409/300‑1 (MQ=255)
                         gTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCAAGTAATACCTGTTGCCACGGCGCGGTTATCGGGAAATGCAGCAGCATTTGATCCACTACGCTGTCGCAGTTGCcgcc                                                                                                                                                                                                                                                                            >  2:284162/1‑300 (MQ=255)
                           gCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTGTTGCCACGGCGCGGTTATCGGGAACTGCAGCAGCATTTGATCCACTTCGCTTTCCCAGTTCCcgcccc                                                                                                                                                                                                                                                                          >  1:433444/1‑298 (MQ=255)
                            cGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTGTTGCCACGGCGCGGTTATCGGGAAATGCAGCAGCATTTGATCCACTTCGCTCTCgagatcggaagagcg                                                                                                                                                                                                                                                                         >  1:393244/1‑287 (MQ=255)
                                 acacTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTGTTGCCACGGCGCGGTTATCGGGAAATGCAGCAGCATTTGATCCACTTCGCTGTCGCAGTTGCCGCGCACAGCGa                                                                                                                                                                                                                                                                    >  2:615992/1‑301 (MQ=255)
                                  tcttAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTGTTGCCACGGCGCGGTTATCGGGAAATGCAGCAGCATTTGATCCACTTCGCTGTCGCAGTTGCCGCGCACAGCGAt                                                                                                                                                                                                                                                                   <  1:393435/298‑1 (MQ=255)
                                     tAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTGTTGCCACGGCGCGGTTATCGGGAAATGCAGCAGCATTTGATCCACTTCGCTGTCGCAGTTGCCGCGCACAGCAATaga                                                                                                                                                                                                                                                                >  2:393949/1‑299 (MQ=255)
                                           ccgatctATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTGTTGCCACGGCGCGGTATCGGGAAATGCAGCAGCATTTGATCCACTTCGCTGTCGCAGTTGCCGCGCACAGCGATAACCTTAtgt                                                                                                                                                                                                                                                         <  2:163410/295‑1 (MQ=255)
                                                  aTCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTGTTGCCACGGCGCGGTATCGGGAAATGCAGCAGCATTTGATCCACTTCGCTGTCGCAGTTGCCGCGCACAGCGATAACCTTAtgtaaatcgg                                                                                                                                                                                                                                                  >  1:163258/1‑294 (MQ=255)
                                                           ccttttgctcttccgatctccGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTTTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTGTTGCCACGGCGCGGTTATCGGGAAATGCAGCAGCATTTGATCCACTTCGCTGTCGCAGTTGCCGCGCACAGCGATAACCTTATGTGCCACTTCATTAAGc                                                                                                                                                                                                                                          <  2:517486/282‑1 (MQ=255)
                                                            ccATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTGTTGCCACGGCGCGGTTATCGGGAAATGCAGCAGCATTTGATCCACTTCGCTGTCGCAGTTGCCGCGCACAGCGATAACCTTATGTACCACTTCATTAAGcc                                                                                                                                                                                                                                         >  1:560893/1‑301 (MQ=255)
                                                                             aCCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAACAACAGACGTTGTTTTTCCAGTAATACCTGTTGCCACGGCGCGGTTATCGGGAAATGCAGCAGCATTTGATCCACTTCGCTGTCGCAGTTGCCGCGCACAGCGATAACCTTATGTGCCACTTCATTAAGCCGTTCAGCAACTTtggct                                                                                                                                                                                                                        >  1:133755/1‑301 (MQ=255)
                                                                             aCCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTGTTGCCACGGCGCGGTTATCGGGAAATGCAGCAGCATTTGATCCACTTCGCTGTCGCAGTTGCCGCGCACAGCGATAACCTTATGTGCCACTTCATTAAGCCGTTCAGCGACTTtggct                                                                                                                                                                                                                        >  2:439822/1‑301 (MQ=255)
                                                                              ccGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTGTTGCCACGGCGCGGTTATCGGGAAATGCAGCAGCATTTGATCCACTTCGCTGTCGCAGTTGCCGCGCACAGCGATAACCTTATGTACCACTTAATTAAGcagatcggaagaacgtcggg                                                                                                                                                                                                                       >  1:516814/1‑282 (MQ=255)
                                                                                             cTCACCGGGCCGTGGTTGAAGTTGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTGTTGCCACGGCGCGGTATCGGGAAATGCAGCAGCATTTGATCCACTTCGCTGTCGCAGTTGCCGCGCACAGCGATAACCTTATGTGCCACTTCATTAAGCCGTTCAGCGACTTTGGCTGGCGCGTAACCCTCCgg                                                                                                                                                                                                       <  1:461403/301‑1 (MQ=255)
                                                                                             cTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTGTTGCCACGGCGCGGTTATCGGGAAATGCAGCAGCATTTGATCCACTTCGCTGTCGCAGTTGCCGCGCACAGCGATAACCTTATGTGCCACTTCATTAAGCCGTTCAGCGACTTTGGCTGGCGCGTAAccccacg                                                                                                                                                                                                        >  1:599828/1‑297 (MQ=255)
                                                                                             cTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTGTTGCCACGGCGCGGTTATCGGGAAATGCAGCAGCATTTGATCCACTTCGCTGTCGCAGTTGCCGCGCACAGCGATAACCTTATGTGCCACTTCATTAAGCCGTTCAGCGACTTTGGCTGGCGCGTAACCCTCCg                                                                                                                                                                                                        <  2:133880/301‑1 (MQ=255)
                                                                                             cTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTGTTGCCACGGCGCGGTTATCGGGAAATGCAGCAGCATTTGATCCACGTCGCTGTCGCAGTTGCCGCGCACAGCGATAACCTTATGTGCCACTTCATTAAGCCGTTCAGCGACTTTGGCTGGCGCGTAACCCTCCg                                                                                                                                                                                                        <  1:439258/301‑1 (MQ=255)
                                                                                                  acagcggggggagattttccacttcaaccccgGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTGTTGCCACGGCGCGGTTATCGGGAAATGCAGCAGCATTTGATCCACTTCGCTGTCGCAGTTGCCGCGCACAGCGATAACCTTATGTGCCACTTCATTAAGCCGTTCAGCGACTTTGGCTGGCGCGTAACCCTCCGGTaaa                                                                                                                                                                                                   >  2:159474/28‑301 (MQ=255)
                                                                                                   gggCCGGGGTTTAAGTGGAAAATCGCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTGTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTGTTGCCACGGCGCGGTTATCGGGAAATGCAGCAGCATTTGATCCACTTCGCTGTCGCAGTTGCCGCGCACAGCGATAACCTTATGTGCCACTTCATTAAGCCGTTCAGCGACTTTGGCTGGCGCGTAACCCTCCGGTAAAg                                                                                                                                                                                                  <  1:421720/299‑1 (MQ=255)
                                                                                                           tttGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAACAGACGTTGTTTTTCCAGTAATACCTGTTGCCACGGCGCGGTTATCGGGAAATGCAGCAGCATTTGATCCACTTCGCTGTCGCAGTTGCCGCGCACAGCGATAACCTTATGTGCCACTTCATTAAGCCGTTCAGCGACTTTGGCTGGCGCGTAACCCTCCGGTAAAGCATTAcgcg                                                                                                                                                                                         <  2:62595/300‑1 (MQ=255)
                                                                                                                  tGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACAATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTGTTGCCACGGCGCGGTTATCGGGAAATGCAGCAGCATTTGATCCACTTAGCTGTCGCAGTTGCCGCGCACAGCGATAACCTTATGTGCCACTTCATTAAGCCGTTCAGCGACTTTGGCTGGCGCGTAACCCTCCGGTAAAGCATAACGCggaacgt                                                                                                                                                                                   >  2:501699/1‑296 (MQ=255)
                                                                                                                          ttcCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTGTTGCCACGGCGCGGTTATCGGGAAATGCAGCAGCATTTGATCCACTTCGCTGTCGCAGTTGCCGCGCACAGCGATAACCTTATGTGCCACTTCATTAAGCCGTTCAGCGACTTTGGCTGGCGCGTAACCCTCCGGTAAAGCATTACGCGGGCCGTGATTCAac                                                                                                                                                                           <  2:434008/300‑1 (MQ=255)
                                                                                                                                 cGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTGTTGCCACGGCGCGGTTATCGGGAAATGCAGCAGCATTTGATCCACTTCGCTGTCGCAGTTGCCGCGCACAGCGATAACCTTATGTGCCACTTCATTAAGCCGTTCAGCGACTTTGGCTGGCGCGTAACCCCCCGGTAAAGCATTACGCGGGCCGTGATTCAACCCGTCGc                                                                                                                                                                    >  1:274313/1‑301 (MQ=255)
                                                                                                                                             ttgtgctcttccgatctaCCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGCTTTTCCAGTAATACCTGTTGCCACGGCGCGGTTATCGGGAAATGCAGCAGCATTTGATCCACTTCGCTGTCGCAGTTGCCGCGCACAGCGATAACCTTATGTGCCACTTCATTAAGCCGTTCAGCGACTTTGGCTGGCGCGTAACCCTCCGGTAAAGCATTACGCGGGCCGTGATTCAACACGTCGCCAAGGATCACCa                                                                                                                                                        <  2:230243/284‑1 (MQ=255)
                                                                                                                                             aCTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTGTTGCCACGGCGCGGTTATCGGGAAATGCAGCAGCATTTGATCCACTTCGCTGTCGCAGTTGCCGCGCACAGCGATAACCTTATGTGCCACTTCATTAAGCCGTTCAGCGACTTTGGCTGGCGCGTAACCCTCCGGTAAAGCATTACGCGGGCCGTGATTCAACACGTCGCCAAGGATCACCa                                                                                                                                                        <  2:600589/301‑1 (MQ=255)
                                                                                                                                              ggggaagagtggTATGACCGTACACAAGCACATCGTTCGGGTTTAAAGCAGGCAGATTTTCCGGTCCAAAAAGATGACCATGCGTCAAAAGCGGACGTTGTTTTTCCAGTAATACCTGTTGCCACGGCGCGGTTATCGGGAAATGCAGCAGCATTTGATCCACTTCGCTGTCGCAGTTGCCGCGCACAGCGATAACCTTATGTGCCACTTCATTAAGCCGTTCAGCGACTTTGGCTGGCGCGTAACCCTCCGGTAAAGCATTACGCGGGCCGTGATTCAACACGTCGCCAAGGATCACCAg                                                                                                                                                       <  1:616016/291‑1 (MQ=255)
                                                                                                                                                      tGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTGTTGCCACGGCGCGGTTATCGGGAAATGCAGCAGCATTTGATCCACTTCGCTGTCGCAGTTGCCGCGCCCAGCGATAACCTTATGTGCCACTTCATTAAGCCGTTCAGCGACTTTGGCTGGCCCGTAACCCCACGGAAACGACTTCCCCCAGCCGCGATTCACCACATCGCCAACGAACCCCaaaccctcgg                                                                                                                                               >  1:553207/1‑292 (MQ=255)
                                                                                                                                                            tGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTGTTGCCACGGCGCGGTTATCGGGAAATGCAGCAGCATTTGATCCACTTCGCTGTCGCAGTTGCCGCGCACAGCGATAACCTTATGTGCCACTTCATTAAGCCGTTCAGCGACTTTGGCTGGCGCGTAACCCTCCGGTAAAGCATTACGCGGGCCGTGATTCAACACGTCACCAAGGAACACAAGCCACCGGGCACCGc                                                                                                                                         >  1:115836/1‑301 (MQ=255)
                                                                                                                                                              aCCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGCTTTTCCAGTAATACCTGTTGCCACGGCGCGGTTATCGGGAAATGCAGCAGCATTTGATCCACTTCGCTGTCGCAGTTGCCGCGCACAGCGATAACCTTATGTGCCACTTCATTAAGCCGTTCAGCGACTTTGGCTGGCGCGTAACCCTCCGGTAAAGCATTACGCGGGCCGTGATTCAACACGTCGCCAAGGATCACCaagatagcaagagcgtcg                                                                                                                                       >  1:230000/1‑284 (MQ=255)
                                                                                                                                                                 ttACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTGTTGCCACGGCGCGGTTATCGGGAAATGCAGCAGCATTTGATCCACTTCGCTGTCGCAGTTGCCGCGCACAGCGATAACCTTATGTGCCACTTCATTAAGCCGTTCAGCGACTTTGGCTGGCGCGTAACCCTCCGGTAAAGCATTACGCGGGCCGTGATTCAACACGTCGCCAAGGATCACCAGCCACTGGGCACCGCTTTgg                                                                                                                                    <  2:115960/300‑1 (MQ=255)
                                                                                                                                                                  tACTCCATCATATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGTTGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTGTTGCCACGGCGCGGTTATCGGGAAATGCAGCAGCATTTGATCCACTTCGCTGTCGCAGTTGCCGCGCACAGCGATAACCTTATGTGCCACTTCATTAAGCCGTTCAGCGACTTTGGCTGGCGCGTAACCCTCCGGTAAAGCATTACGCGGGCCGTGATTCAACACGTCGCCAAGGATCACCAGCCACTGGGCACCGCTTTggg                                                                                                                                   <  2:460107/301‑1 (MQ=255)
                                                                                                                                                                      ccAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTGTTGCCACGGCGCGGTTATCGGGAAATGCAGCAGCATTTGATCCACTTCGCTGTCGCAGTTGCCGCGCACAGCGATAACCTTATGTGCCACTTCATTAAGCCGTTCAGCGACTTTGGCTGGCGCGTAACCCTCCGGTAAAGCATTACGCGGGCCGTGATTCAACACGTCGCCAAGGATCACCAGCCACTGGGCACCGCTTTGGGCaaa                                                                                                                               >  1:277911/1‑301 (MQ=255)
                                                                                                                                                                       cAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTGTTGCCACGGCGCGGTTATCGGGAAATGCAGCAGCATTTGATCCACTTCGCTGTCGCAGTTGCCGCGCACAGCGATAACCTTATGTGCCACTTCATTAAGCCGTTCAGCGACTTTGGCTGGCGCGTAACCCTCCGGTAAAGCATTACGCGGGCCGTGATTCAACACGTCGCCAAGGATCACCAGCCACTGGGCACCGCTTTGGGCAaac                                                                                                                              <  2:561607/301‑1 (MQ=255)
                                                                                                                                                                          cactTCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGTGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTGTTGCCACGGCGCGGTTATCGGGAAATGCAGCAGCAGTTGATCCACTTCGCTGTCGCAGTTGCCGCGCACAGCGATAACCTTATGTGCCACTTCATTAAGCCGTTCAGCGACTTTGGCTGGCGCGTAACCCTCCGGTAAAGCATTACGCGGGCCGTGATTCAACACGTCGCCAAGGATCACCAGCCACTGGGCACCGCTTTGGGCAaacaac                                                                                                                           <  1:207292/301‑1 (MQ=255)
                                                                                                                                                                            caTCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTGTTGCCACGGCGCGGTTATCGGGAAATGCAGCAGCATTTGATCCACTTCGCTGTCGCAGTTGCCGCGCACAGCGATAACCTTATGTGCCACTTCATTAAGCCGTTCAGCGACTTTGGCTGGCGCGTAACCCTCCGGTAAAGCATTACGCGGGCCGTGATTCAACACGTCCCCAAGGATCACCAGCCACTGGGCACCGCTTTGGGCAaacaaccc                                                                                                                         >  1:342723/1‑299 (MQ=255)
                                                                                                                                                                              atgtccgatattAAAGTAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGGCAAAAACAGACGTTGTTTTTCCAGTAATACCTGTGGCCACGGCGCGGTTATCGGGAAATGCAGCAGCATTTGATCCACTGCGCTGTCGCAGTTGCCGCGCACAGCGATAACCTTATGTGCCACTTCATTAAGCCGTTCAGCGACTTTGGCTGGCGCGTAACCCTCCGGTAAAGCATTACGCGGGCCGTGATTCAACACGTCGCCAAGGATCACCAGCCACTGGGCACCGCTTTGGGCAAACAACTCCa                                                                                                                       <  1:448759/291‑1 (MQ=255)
                                                                                                                                                                               cGTTCTGGTTTAAAGCAGGCAGGTTTTTCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTGTTGCCACGGCGCGGTTATCGGGAAAGTCAGCAGCATTTGATCCACTTCGCTGTCGCAGTTGCCGCGCACAGCGATAACCTTATGTGCCACTTCATTAAGCCGTTCAGCGACTTTGGCTGGCGCGTAACCCTCCGGTAAAGCATTACGCGGGCCGTGATTCAACACGTCGCCAAGGATCACCAGCCACTGGGCACCGCTTTGGGCAAACAACTCCAg                                                                                                                      <  1:198513/301‑1 (MQ=255)
                                                                                                                                                                                       tttAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTGTTGCCACGGCGCGGTTATCGGGAAATGCAGCAGCATTTGATCCACTTCGCTGTCGCAGTTGCCGCGCACAGCGATAACCTTATGTGCCACTTCATTAAGCCGTTCAGCGACTTTGGCTGGCGCGTAACCCTCCGGTAAAGCATTACGCGGGCCGTGATTCAACACGTCGCAAAGGATCACCAGCCACTGGGACACGCTTTAGGCAAACAACCCCAAAacaccat                                                                                                              >  1:544554/1‑298 (MQ=255)
                                                                                                                                                                                         tAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTGTTGCCACGGCGCGGTTATCGGGAAATGCAGCAGCATTTGATCCACTTCGCTGTCGCAGTTGCCGCGCACAGCGATAACCTTATGTGCCACTTCATTAAGCCGTTCAGCGACTTTGGCTGGCGCGTAACCCTCCGGTAAAGCATTACGCGGGCCGTGATTCAACACGTCGCCAAGGAACCCCAGCCACTGGCCACCGCTTTGAGCAACCAAATCCAGAACACGTAcc                                                                                                            >  2:111653/1‑301 (MQ=255)
                                                                                                                                                                                         tAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTGTTGCCACGGCGCGGTTATCGGGAAATGCAGCAGCATTTGATCCACTTCGCTGTCGCAGTTGCCGCGCACAGCGATAACCTTATGTGCCACTTCATTAAGCCGTTCAGCGACTTTGGCTGGCGCGTAACCATCCGGTAAAGCATTACGCGGGCCGTGATTCAACACGTCGCCAAGGATCACCAGCCACTGGGCACCGCTTTGGGCAAAAAACTCCaagatcggaaga                                                                                                            >  2:449323/1‑290 (MQ=255)
                                                                                                                                                                                         aaaaGGAGGCGGTTTTTCCTGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAGTACCTGTTGCCACGGCGCGGTTATCGGGAAATGCATCAGCATTTGATCCACTTCGCTGTCGCAGTTGCCGCGCACAGCGATAACCTTATGTGCCACTTCATTAAGCCGTTCAGCGACTTTGGCTGGCGCGTAACCCTCCGGTAAAGCATTACGCGGGCCGTGATTCAACACGTCGCCAAGGATCACCAGCCACTGGGCACCGCTTTGGGCAAACAACTCCAGAACACGTTcc                                                                                                            <  1:283859/300‑1 (MQ=255)
                                                                                                                                                                                               aggtattttttCCGGTCCATGAAGATGACCATGCGTCAATACCAGACGTTGTGTTTCCCGTATGACCTGTTGCCACGGTGCGTTTATCGTGAAATGCAGCAGCATTTGTTCCACTTCGCTGTCGCAGTTGCCGCGCACAGCGATACCCTTATGTGCCACTTCATTAAGCCGTTCAGCGACTTTGGCTCGCGCGTAACCCTCCGGTAAAGCATTACGCGGGCCGTGATTCAACACGTCGCCAAGGATCACCATCCACTGGGCACCGCTTTGGGCAAACAACTCCAGAACACGTTCCGTCGcc                                                                                                      <  1:501052/294‑1 (MQ=255)
                                                                                                                                                                                               aggcagATTTTCCGGGCCAAAAAGATTACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTGTTGCCACGGCGCGGTTATCGGGAAATGCAGCAGCATTTGATCCACTTCGCTGTCGCAGTTGCCGCGCACAGCGATAACCTTATGTGCCACTTCATTAAGCCGTTCAGCGACTTTGGCTGGCGCGTAACCCTCCGGTAAAGCATTACGCGGGCCGTGATTCAACACGTCGCCAAGGATCACCAGCCACTGGGCACCGCTTTGGGCAAACAACTCCAGAACACGTTCCGTCGcc                                                                                                      <  2:343186/301‑1 (MQ=255)
                                                                                                                                                                                               aggcagATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTGTTGCCACGGCGCGGTTATCGGGAAATGCAGCAGCATTTGATCCACTTCGCTGTCGCAGTTGCCGCGCACAGCGATAACCTTATGTGCCACTTCATTAAGCCGTTCAGCGACTTTGGCTGGCGCGTAACCCTCCGGTAAAGCATTACGCGGGCCGTGATTCAACACGTCGCCAAGGATCACCAGCCACTGGGCACCGCTTTGGGCAAACAACTCCAGAACACGTTCCGTCGcc                                                                                                      <  2:545227/301‑1 (MQ=255)
                                                                                                                                                                                                ggcagATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTGTTGCCACGGCGCGGTTATCGGGAAATGCAGCAGCATTTGATCCACTTCGCTGTCGCAGTTGCCGCGCACAGCGATAACCTTATGTGCCACTTCATTAAGCCGTTCAGCGACTTTGGCTGGCGCGTAACCCTCCGGTAAAGCATTACGCGGGCCGTGATTCAACACGTCGCCAAGGATCACCAGCCACTGGGCACCGCTTTGGGCAAAAAACTACAAAAACAGTTCCGTCGCCg                                                                                                     >  2:452721/1‑301 (MQ=255)
                                                                                                                                                                                                           cGGGCCAAAAAGTTGACCTGTCTTCAAAACGTGTCGTTTTTTTTCCAGTATTACGTGTTGCCACGGCGCGGTTATCGGGAAAGGCGGCAGCATTTGATCCACTTCGCTGGCGCAGTGGCCGCGCACAGCGAGAACCTTATGTGCCACTTCATTAAGCCGTTCAGCGACTTTGGCTGGCGCGGAACCCTCCGGTAAAGCATTACGCGGGCCGTGATTCAACACGTCGCCAAGGATCACCAGCCACTGGGCACCGCTTTGGGCAAACAACTCCAGAACACGTTCCGTCGCCGGTAACGACCCa                                                                                          <  1:452157/301‑1 (MQ=255)
                                                                                                                                                                                                                           ccATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTGTTGCCACGGCGCGGTTATCGGGAAATGCAGCAGCATTTGATCCACTTCGCTGTCGCAGTTGCCGCGCACAGCGATAACCTTATGTGCCACTTCATTAAGCCGTTCAGCGACTTTGGCTGGCGCGTAACCCTCCGGTAAAGCATTACGCGGGCCGTGATTCAACACGTCGCCAAGGATCACCAGCCACTGGGCACCGCTTTGGGCAAACAACTCCAGAACACGTTCCGTCGCCGGTAACGACCCATGAATGTCCGATGCaa                                                                          >  2:613048/1‑301 (MQ=255)
                                                                                                                                                                                                                                             cGTTGTTTTTCCAGTAATACCTGTTGCCACGGCGCGGTTATCGGGAAATGCAGCAGCATTTGATCCACTTCGCTGTCGCAGTTGCCGCGCACAGCGATAACCTTATGTGCCACTTCATTAAGCCGTTCAGCGACTTTGGCTGGCGCGTAACCCTCCGGTAAAGCATTACGCGGGCCGTGATTCAAAACGTCGCCAAGGATCACCAGCCACTGGGCACCGCTTTGGGCAAACAACTCCAGAACACGTTCCGTCGCCGGTCACGACCCATGAATATCCGACCCAAACAACAGCTTCAtcaccc                                                        >  1:185386/1‑299 (MQ=255)
                                                                                                                                                                                                                                                  tttttCCAGTAATACCTGTTGCCACGGCGCGGTTATCGGGAAATGCAGCAGCATTTGATCCACTTCGCTGTCGCAGTTGCCGCGCACAGCGATAACCTTATGTGCCACTTCATTAAGCCGTTCAGCGACTTTGGCTGGCGCGTAACCCTCCGGTAAAGCATTACGCGGGCCGTGATTCAACACGTCGCCAAGGATCACCAGCCACTGGGCACCGCTTTGGGCAAACAACTCCAGAACACGTTCCGTCGCCGGTAACGACCCATGAATGTCCGATGCAAACAACAGTTTAATCACTCAcccc                                                   >  2:234453/1‑301 (MQ=255)
                                                                                                                                                                                                                                                       ccAGTAAGACATGTTGTCACGTGGAGGTGATCGGGAAATGCAGCAGCATTTGATCCACTTCGCTGTCACAGTTGCCGCGAACAGCGAGAACCTTATGTGCACCTTCAGTAAGCCGTGCAGCGACTTTGGCTGGCGCGTAACCCTCCGGTAAAGCATTACGCGGGCCGTGATTCAACACGTCGCCAAGGATCACCAGCCACTGGGCACCGCTTTGGGCAAACAACTCCAGAACACGTTCCGTCGCCGGTAACGACCCATGAATGTCCGATGCAAACATCAGTTTCATCACTCACTCCtcgtc                                              <  1:444930/301‑1 (MQ=255)
                                                                                                                                                                                                                                                         aGTAATGCCTGTTGCCACGGCGCGGTTATCGGGAAATGCAGCAGCATTTGATCCACTTCGCTGTCGCAGTGGACGCGCACAGGGATAACCTTATGTGCCACTTCATTAAGCCGTTCAGCGACTTTGGCGGGCGCGTAACCCTCCGGTAAAGCATTACGCGGGCCGTGATTCAACACGTCGCCAAGGATCACCAGCCACTGGGCACCGCTTTGGGCAAACAACTCCAGAACACGTTCCGTCGCCGGTAACGACCCATGAATGTCCGATGCAAACATCAGTTTCATCACTCACTCCTCGTCGa                                            <  1:615134/301‑1 (MQ=255)
                                                                                                                                                                                                                                                                   gTTGCCACGGCGCGGTTATCGGGAAATGCAGCAGCATTTGATCCACTTCGCTGTCGCAGTTGCCGCGCACAGCGATAACCTTATGTGCCACTTCATTAAGCCGTTCAGCGACTTTGGCTGGCGCGTAACCCTCCGGTAACGCATTACGCGGGCCGTGATTCAACACGTCGCCAAGGATCACCAGCCACTGGGCACCGCTTTGGGCAAACAACTCCAGACCACGTTCCGTCGCCGGTCACGACCCATGAATGTCCGATGCAAACATCCGTTTAATCCACCACACCCCGTCCAAAAAAcacaa                                  >  1:493547/1‑297 (MQ=255)
                                                                                                                                                                                                                                                                      gcctcggtcgggTTATCGGGAAATTCGGCTGCCTTTGATCCACTTCTCTTGCGCAGTTGCCGCGCACAGCGATAACCTTATGTGCCACTTCATTAAGCCGTTCAGCGACTTTGGCTGGCGCGTAACCCTCCGGTAAAGCATTACGCGGGCCGTGATTCAACACGTCGCCAAGGATCACCAGCCACTGGGCACCGCTTTGGGCAAACAACTCCAGAACACGTTCCGTCGCCGGTAACGACCCATGAATGTCCGATGCAAACATCAGTTTCATCACTCACTCCTCGTCGAAAAAACGTAAGCt                               <  1:612190/291‑1 (MQ=255)
                                                                                                                                                                                                                                                                      gCCACGGCGCGGTTATCGGGAAATGCAGCAGCATTTGATCCACTTCGCTGTCGCAGTTGCCGCGCACAGCGATAACCTTATGTGCCACTTCATTAAGCCGTTCAGCGACTTTGGCTGGCGCGTAACCCTCCGGTAAAGCATTACGCGGGCCGTGATTCAACACGTCGCCAAGGATCACCAGCCACTGGGCACCGCTTTGGGCAAACAACTCCAGAACACGTTCCGTCGCCGGTAACGACCCATGAATGTCCGATGCAAACATCAGTTTCATCACTCACTCCTCGTCGAAAAAAAGTAAGca                               >  2:140337/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                        cACGGCGCGGTTATCGGGAAATGCAGCAGCATTTGATCCACTTCGCTGTCGCAGTTGCCGCGCACAGCGATAACCTTATGTGCCACTTCATTAAGCCGTTCAGCGACTTTGGCTGGCGCGTAACCCTCCGGTAAAGCATTACGCGGGCCGTGATTCAACACGTCGCCAAGGATCACCAGCCACTGGGCACCGCTTTGGGCAAACAACTCCAGAACACGTTCCGTCGCCGGTAACGACCCATGAATGTCCGATGCAAACATCAGTTTCATCACCACCTCCTCGTCGAAAAAACGTAAGCTAt                             >  1:103063/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                           ggCGCGGTTATCGGGAAATGCAGCAGCATTTGATCCACTTCGCTGTCGCAGTTGCCGCGCACAGCGATAACCTTATGTGCCACTTCATTAAGCCGTTCAGCGACTTTGGCTGGCGCGTAACCCTCCGGTAAAGCATTACGCGGGCCGTGATTCAACACGTCGCCAAGGATCACCAGCCACTGGGCACCGCTTTGGGCAAACAACTCCAGAACACGTTCCGTCGCCGGTAACGACCCATGAATGTCCGATGCAAACATCAGTTTCATCACTCACTCCTCGTCGAAAAAACGTAAGCTATGaa                          >  2:594923/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                              gcgGTTATCGGGAAATGCAGCAGCATTTGATCCACTTCGCTGTCGCAGTTGCCGCGCACAGCGATAACCTTATGGGCCACTTCATTAAGCCGTTCAGCGACTTGGGCTGGCGCGTAACCCTCCGGTAAAGCATTACGCGGGCCGTGATTCAACACGTCGCCAAGGATCACCAGCCACTGGGCACCGCTTTGGGCAAACAACTCCAGAACACGTTCCGTCGCCGGTAACGACCCATGAATGTCCGATGCAAACATCAGTTTCATCACTCACTCCTCGTCGAAAAAACGTAAGCTATGATACt                       <  1:278592/301‑1 (MQ=255)
                                                                                                                                                                                                                                                                                ggTTATCGGGAAATGCAGCAGCATTTGATCCACTTCGCTGTCGCAGTTGCCGCGCACAGCGATAACCTTATGTGCCACTTCATTAAGCCGTTCAGCGACTTTGGCTGGCGCGTAACCCTCCGGTAAAGCATTACGCGGGCCGTGATTCAACACGTCGCCAAGGATCACCAGCCACTGGGCACCGCTTTGGGCAAACAACTCCAGAACACGTTCCGTCGCCGGTAACGACCCATGAATGTCCGATGCAAACATCAGTTTCATCACTCACTCCACGTCGAAAAAACATAAGCCATGATACTgg                     >  1:493643/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                                     gcGGGAAATGCTGCTGCATTTGTTCCACTTCGCTGTCGCAGTTGCCGCGCACAGCGATAACCTGATGTGCCACTTCATTAAGCCGTTCAGCGACTTTGGCTGGCGCGTAACCCTCCGGTAAAGCATTACGCGGGCCGTGATTCAACACGTCGCCAAGGATCACCAGCCACTGGGCACCGCTTTGGGCAAACAACTCCAGAACACGTTCCGTCGCCGGTAACGACCCATGAATGTCCGATGCAAACATCAGTTTCATCACTCACTCCTCGTCGAAAAAACGTAAGCTATGATACTGGATTCt                <  1:594162/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                                      gttcaaaTTCTGCTGCATTTGATCCATTTCGCTGTCGCAGTTGCCGCGCACAGCGATAACCTTATGTGCCACTTCATTAAGCCGTTCAGCGACTTTGGCTGGCGCGTAACCCTCCGGTAAAGCATTACGCGGGCCGTGATTCAACACGTCGCCAAGGATCACCAGCCACTGGGCACCGCTTTGGGCAAACAACTCCAGAACACGTTCCGTCGCCGGTAACGACCCATGAATGTCCGATGCAAACATCAGTTTCATCACTCACTCCTCGTCGAAAAAACGTAAGCTATGATACTGGATTCTg               <  2:418014/297‑1 (MQ=255)
                                                                                                                                                                                                                                                                                      gatcaaaTGCTGTTGCATTTGTTCCACTTCGCTGTCGCAGTTGCCGCGCACAGCGATAACCTTCTGTGCCACTTCATTAAGCCGTTCAGCGACTTTGGCTGGCGCGTAACCCTCCGGTAAAGCATTACGCGGGCCGTGATTCAACACGTCGCCAAGGATCACCAGCCACTGGGCACCGCTTTGGGCAAACAACTCCAGAACACGTTCCGTCGCCGGTAACGACCCATGAATGTCCGATGCAAACATCAGTTTCATCACTCACTCCTCGTCGAAAAAACGTAAGCTATGATACTGGATTCTg               <  2:478298/297‑1 (MQ=255)
                                                                                                                                                                                                                                                                                      gatcaaaTGCTGCTGCATTTGATCCACTTCGCTGTCGCAGTTGCCGCGCACAGCGATAACCTTATGTGCCACTTCATTAAGCCGTTCAGCGACTTTGGCTGGCGCGTAACCCTCCGGTAAAGCATTACGCGGGCCGTGATTCAACACGTCGCCAAGGATCACCAGCCACTGGGCACCGCTTTGGGCAAACAACTCCAGAACACGTTCCGTCGCCGGTAACGACCCATGAATGTCCGATGCAAACATCAGTTTCATCACTCACTCCTCGTCGAAAAAACGTAAGCTATAATACTGGATTCtt               >  2:186683/5‑300 (MQ=255)
                                                                                                                                                                                                                                                                                      gatcaaaTGCTGCTGCATTTGATCCACTTCGCTGTCGCAGTTGCCGCGCACAGCGATAACCTTATGTGCCACTTCATTAAGCCGTTCAGCGACTTTGGCTGGCGCGTAACCCTCCGGTAAAGCATTACGCGGGCCGTGATTCAACACGTCGCCAAGGATCACCAGCCACTGGGCACCGCTTTGGGCAAACAACTCCAGAACACGTTCCGTCGCCGGTAACGACCCATGAATGTCCGATGCAAAAATCAGTTTCATCACTCACTCCTCGTCGAAAAAACGCAAGCTATGATACTGGATTCtt               >  1:417451/5‑300 (MQ=255)
                                                                                                                                                                                                                                                                                      cGGGAAATGCAGCAGCATTTGATCCACTTCGCTGTCGCAGTTGCCGCGCACAGCGATAACCTTATGTGCCACTTCATTAAGCCGTTCAGCGACTTTGGCTGGCGCGTAACCCTCCGGTAAAGCATTACGCGGGCCGTGATTCAACACGTCGCCAAGGATCACCAGCCACTGGGCACCGCTTTGGGCAAACAACTCCAGAACACGTTCCGTCGCCGGTAACGACCCATGAATGTCCGATGCAAACATCAGTTTCATCACTCACTCCTCGTCGAAAAAACGTAAGCTATGATACTGGATTCTg               >  2:240905/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                                               cagcagcaTTTGATCCACTTCGCTGTCGCAGTTGCCGCGCACAGCGATAACCTTATGTGCCACTTCATTAAGCCGTTCAGCGACTTTGGCTGGCGCGTAACCCTCCGGTAAAGCATTACGCGGGCCGTGATTCAACACGTCGCCAAGGATCACCAGCCACTGGGCACCGCTTTGGGCAAACAACTCCAGAACACGTTCCGTCGCCGGTAACGACCCATGAATGTCCGATGCAAACATCAGTTTCATCACTCACCCCCCGTCGCAAAAACGTAAGCTATGATACTGGATTCTGTCTTTGCtt      >  1:13930/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                                                  gagctTTTGATCCACTGCGCTGTCGCAGTGGCCGCGAACAGCGATAACCTTATGTGCCATTTCATTAAGCCGTTCAGCGACTTTGGCTGGCGCGTAACCCTCCGGTAAAGCATTACGCGGGCCGTGATTCAACACGTCGCCAAGGATCACCAGCCACTGGGCACCGCTTTGGGCAAACAACTCCAGAACACGTTCCGTCGCCGGTAACGACCCATGAATGTCCGATGCAAACATCAGTTTCATCACTCACTCCTCGTCGAAAAAACGTAAGCTATGATACTGGATTCTGTCGTTGCTATCa   <  1:240638/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                                                   agcaTTTGATCCACTTCGCTGTCGCAGTTGCCGCGCACAGCGATAACCTTATGTGCCACTTCATTAAGCCGTTCAGCGACTTTGGCTGGCGCGTAACCCTCCGGTAAAGCATTACGCGGGCCGTGATTCAACACGTCGCCAAGGATCACCAGCCACTGGGCACCGCTTTGGGCAAACAACTCCAGAACACGTTCCGTCGCCGGTAACGACCCATGAATGTCCGATGCAAACATCAGTTTCATCACTCACTCCTCGTCGAAAAAACGTAAGCTATGATACTGGATTCTGTCGTTGCTATCAg  <  2:544968/301‑1 (MQ=255)
                                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                                                            
TGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTGTTGCCACGGCGCGGTTATCGGGAAATGCAGCAGCATTTGATCCACTTCGCTGTCGCAGTTGCCGCGCACAGCGATAACCTTATGTGCCACTTCATTAAGCCGTTCAGCGACTTTGGCTGGCGCGTAACCCTCCGGTAAAGCATTACGCGGGCCGTGATTCAACACGTCGCCAAGGATCACCAGCCACTGGGCACCGCTTTGGGCAAACAACTCCAGAACACGTTCCGTCGCCGGTAACGACCCATGAATGTCCGATGCAAACATCAGTTTCATCACTCACTCCTCGTCGAAAAAACGTAAGCTATGATACTGGATTCTGTCGTTGCTATCAG  >  NC_000913/2419253‑2419844

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: