Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 847,213 A→T 8.2% intergenic (‑94/+45) glnP ← / ← glnH glutamine transporter subunit/glutamine transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913847,2130AT 8.2% 134.5 / 4.1 49intergenic (‑94/+45)glnP/glnHglutamine transporter subunit/glutamine transporter subunit
Reads supporting (aligned to +/- strand):  ref base A (20/25);  new base T (2/2);  total (22/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.24e-01

GTGTGCCGCGGATCACTTCAATAAAGACCAGCGCGACGTGGTTGGCTATCCAACCTCCGAAGGTGCGTGCAAAACCTGCCAGCAATCCGATTACCAGACCGCCTGCCAGACCGAGGACCGAAATCCACAGGGTCATTTTGGCACCTTCAATCAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGTCACGCAGCTCGTCGCTACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGGTTTTGATGAAGTACAGAATGTTTGGCGTATCGTGCAGAACGGCGTCTGCGCGGTTGGTGCCCAGTTCCAT  >  NC_000913/846924‑847509
                                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                                        
gggtgCCGCGTATCACTTCAATAAAGACCAGCGCGACGTGGTTGGCTATCCAACCTCCGAAGGTGCGTGCAAAACCTGCCAGCAATCCGATTACCAGACCGCCTGCCAGACCGAGGACCGAAATCCACAGGGTCATTTTGGCACCTTCAATCAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAgg                                                                                                                                                                                                                                                                                               <  1:135633/299‑1 (MQ=255)
  ttgCCGCGGATCACTTCAATAAAGACCAGCGCGACGTGGTTGGCTATCCAACCTCCGAAGGTGCGTGCAAAACCTGCCAGCAATCCGATTACCAGACCGCCTGCCAGACCGAGGACCGAAATCCACAGGGTCATTTTGGCACCTTCAATCAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCTATGGGGAGAggat                                                                                                                                                                                                                                                                                             <  2:52053/300‑3 (MQ=255)
   tgCCGCGGATCACTTCAATAAAGACCAGCGCGACGTGGTTGGCTATCCAACCTCCGAAGGTGCGTGCAAAACCTGCCAGCAATCCGATTACCAGACCGCCTGCCAGACCGAGGACCGAAATCCACAGGGTCATTTTGGCACCTTCAATCAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCCCCCCATAGGGGAGAGGGaa                                                                                                                                                                                                                                                                                            >  2:191074/1‑301 (MQ=255)
     ccGCGGATCACTTCAATAAAGACCAGCGCGACGTGGTTGGCTATCCAACCTCCGAAGGTGCGTGCAAAACCTGCCAGCAATCCGATTACCAGACCGCCTGCCAGACCGAGGACCGAAATCCACAGGGTCATTTTGGCACCTTCAATCAGAAGCGGAATGGTAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATAAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCTATGGGGAGAggattag                                                                                                                                                                                                                                                                                          <  2:545899/301‑6 (MQ=255)
                ttCAATAAAGACCAGCGCGACGTGGTTGGCTATCCAACCTCCGAAGGTGCGTGCAAAACCTGCCAGCAATCCGATTACCAGACCGCCTGCCAGACCGAGGACCGAAATCCACAGGGTCATTTTGGCACCTTCAATCAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGtt                                                                                                                                                                                                                                                                               <  1:249516/301‑1 (MQ=255)
                    aTAAAGACCAGCGCGACGTGGTTGGCTATCCAACCTCCGAAGGTGCGTGCAAAACCTGCCAGCAATCCGATTACCAGACCGCCTGCCAGACCGAGGACCGAAATCCACAGGGTCATTTTGGCACCTTCAATCAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATCGGGGAGAGGGAACTGCCAGTGCGTTTTaa                                                                                                                                                                                                                                                                           >  2:508169/1‑300 (MQ=255)
                                    ggTGGTTGGCTATCCAACTGCCGAAGGGGCGGGCAAAACCTGCCAGCAATCCGATTACCAGACCGCCTGCCAGACCGAGGACCGAAATCCACAGGGTCATTTTGGCACCTTCAATCAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATAGGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGttatta                                                                                                                                                                                                                                                           <  1:205738/300‑1 (MQ=255)
                                    ctgggttggttATCCAATCTCCGGAGGTGCGTGCAAAACCGGCCAGCAATCCGATTACCAGACCGCCTGCCAGACCGAGGACCGAAATCCACAGGGTCATTTTGGCACCTGCAATCAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGttatta                                                                                                                                                                                                                                                           <  1:22467/298‑1 (MQ=255)
                                            gCTATCCAACCTCCGAAGGTGCGTGCAAAACCTGCCAGCAATCCGATTACCAGACCGCCTGCCAGACCGAGGACCGAAATCCACAGGGTCATTTTGGCACCTTCAATCAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGtt                                                                                                                                                                                                                                                   <  2:232366/301‑1 (MQ=255)
                                             ctttCCAACCTCCGAAGGTGCGTGCAAAACCTGCCAGCAATCCGATTACCAGACCGCCTGCCAGACCGAGGACCGAAATCCACAGGGTCATTTTGGCACCTTCAATCAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTc                                                                                                                                                                                                                                                  <  1:168208/298‑1 (MQ=255)
                                                  cAACCTCCGAAGGTGCGTGCAAAACCTGCCAGCAATCCGATTACCAGACCGCCTGCCAGACCGAGGACCGAAATCCACAGGGTCATTTTGGCACCTTCAATCAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTAc                                                                                                                                                                                                                                             >  2:25757/1‑301 (MQ=255)
                                                  cAACCTCCGAAGGTGCGTGCAAAACCTGCCAGCAATCCGATTACCAGACCGCCTGCCAGACCGAGGACCGAAATCCACAGGGTCATTTTGGCACCTTCAATCAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTACAGTaa                                                                                                                                                                                                                                             >  2:419175/1‑300 (MQ=255)
                                                  cAACCTCCGAAGGTGCGTGCAAAACCTGCCAGCAATCCGATTACCAGACCGCCTGCCAGACCGAGGACCGAAATCCACAGGGTCATTTTGGCACCTTCAATCAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCACATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTAc                                                                                                                                                                                                                                             >  2:477020/1‑301 (MQ=255)
                                                               tgcgtgcAAAACCTGCCAGCAATCCGATTACCAGACCGCCTGCCAGACCGAGGACCGAAATCCACAGGGTCATTTTGGCACCTTCAATCAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGCGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCAtttatt                                                                                                                                                                                                                                >  2:330536/1‑301 (MQ=255)
                                                                            tGCCAGCAAGCCGATTACCAGACCGCCTGCCAGACCGAGGACCGAAATCCACAGGGTCATTTTGGAACCTTCAAGCAGAAGCGGAATGGCAGGCCAGATGGCAATCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTg                                                                                                                                                                                                                   <  2:323876/301‑1 (MQ=255)
                                                                               cAGCAATCCGATTACCAGACCGCCTGCCAGACCGAGGACCGAAATCCACAGGGTCATTTTGGCACCTTCAATCAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTaa                                                                                                                                                                                                                <  2:59923/301‑1 (MQ=255)
                                                                                            aCCAGACCGCCTGCCAGACCGAGGACCGAAATCCACAGGGTCATTTTGGCACCTTCAATCAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATGTTTTGTAGATTTCGTTGTAAGTTCCGTTCTcgg                                                                                                                                                                                                   >  2:616652/1‑300 (MQ=255)
                                                                                                                 gggACCTAAATCCACAGGGGCATTTTGGCACCTTCAATCATAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGt                                                                                                                                                                              <  1:330131/300‑1 (MQ=255)
                                                                                                                  ggACCGAAATCCACAGGGTCATTTTGGCACCTTCAATCAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGtt                                                                                                                                                                             >  1:426017/1‑301 (MQ=255)
                                                                                                                    aCCGACATCCACAGGGTCAGTTTTGCTCCTTCTATCAGAAGCGTATTGGCAGGCCAGATGTCACGCCAGTCAAACTGCATGTGTTGTTCCTGTTACCGTGGTGTAAATAAGCAAATTCAGGGGAATTAATGGCCCCGGGGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATGTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGa                                                                                                                                                                           <  1:478656/301‑1 (MQ=255)
                                                                                                                                                 ttCAATCAGAAGCGGAATGGCAGGCCAGATGTCATTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGTCACGCAGCTCGTCGCTACCTTTc                                                                                                                                              <  1:476419/301‑1 (MQ=255)
                                                                                                                                                    atgtaGAAGCGGAATGGCAGGCCAGATGGCACTACAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGTCACGCAGCTCGTCGCTACCTTTCggg                                                                                                                                           <  1:507497/297‑1 (MQ=255)
                                                                                                                                                                                   cTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAACTTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGCGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCACCGCGCCGTTGACTTTTTCACGCCGCTCGTCTCTACCTTTCGGGAACCCAATACCGTATTGCTGCGCCTCCagag                                                                                                            >  1:133897/1‑301 (MQ=255)
                                                                                                                                                                                   cTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGTCACGCAGCTCGTCGCTACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTCCagag                                                                                                            <  2:426581/301‑1 (MQ=255)
                                                                                                                                                                                      cAGTCAAACTGCATTTGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATGCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGTCACGCAGCTCGTCGCTACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGt                                                                                                         <  1:25706/301‑1 (MQ=255)
                                                                                                                                                                                           aaaCTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGTCACGCAGCACGTCGCTACATTTCGGGAACACAATACCGTATTTCTACACTACCAGAGAGACaacc                                                                                                    >  2:485806/1‑298 (MQ=255)
                                                                                                                                                                                              ttGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGTCACGCAGCTCGTCGCTACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTAcc                                                                                                 <  2:220533/300‑1 (MQ=255)
                                                                                                                                                                                               tGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGTCACGCAGCTCGTCGCTACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTAccc                                                                                                >  2:625011/1‑300 (MQ=255)
                                                                                                                                                                                                    gtgttgtTCCTGGTACCGTGTGGTAAATTATCAAATTCAGGGTAATGAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGTCACGCAGCTCGTCGCTACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTg                                                                                           <  1:624152/300‑1 (MQ=255)
                                                                                                                                                                                                     tgttgtTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGTCACGCAGCTCGTCGCTACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGa                                                                                          <  2:603966/301‑1 (MQ=255)
                                                                                                                                                                                                      gttgttCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGTCACGCAGCTCGTCGCTACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGaa                                                                                         >  2:406329/1‑301 (MQ=255)
                                                                                                                                                                                                         gttCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGTCCCGCAGCTCGTAGCTACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTCAAGAGAGTCACCTACCGCTTAGAAcgt                                                                                      >  1:414978/1‑299 (MQ=255)
                                                                                                                                                                                                          ttCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGTCACGCAGCTCGTCGCTACCTTTCGTGAACGCAATACCGTATTGCTGCGCTTCCAGCGAGTCACCTCCCGCTTTGAACTgg                                                                                     >  1:541985/1‑300 (MQ=255)
                                                                                                                                                                                                           tCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGTCACGCAGCTCGTCGCTACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGAc                                                                                    <  2:542658/301‑1 (MQ=255)
                                                                                                                                                                                                                            aaaTAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGTCACGCAGCTCGTCGCTACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGAACGTTACCGGCGGtttta                                                                   >  2:466283/1‑300 (MQ=255)
                                                                                                                                                                                                                             atatatctattttaGGGTAATTTATTGCCCCGGAGCGGGTTTGCGCCCTTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATGTCGTTGTAAGTTCCGTTCGCGCGGAGGGTTTTCAACGCGCCGTTGACTTTGTCACGCAGCTCGTCGCTACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGGTTTtga                                                                  <  1:190831/288‑1 (MQ=255)
                                                                                                                                                                                                                                  tCAAAGTCAGGGTATTTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGTCACGCAGCTCGTCGCTACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGGTTTTGATGAAg                                                             <  2:568097/301‑1 (MQ=255)
                                                                                                                                                                                                                                             gTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGTCACGCAGCTCGTCGCTACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGGTTTTGAAGAAGTACAGAATGtt                                                  >  2:622246/1‑301 (MQ=255)
                                                                                                                                                                                                                                                          ccgggtcGGGTTTGGGCCCCTCACCCTAATCCTCTCCCCATAGGGGGGAGGGAATGGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCTACGCTCCGTTGACTTTGTCACGCAGCTCGTCGCTACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGGTTTTGATGAAGTACAGAATGTTTGGCGTATCGTGc                                     <  1:405766/295‑1 (MQ=255)
                                                                                                                                                                                                                                                          ccGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGTCACGCAGCTCGTCGCTACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGGTTTTGATGAAGTACAGAATGTTTGGCGTATCGTGc                                     <  2:415541/301‑1 (MQ=255)
                                                                                                                                                                                                                                                               gCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGTCACGCAGCTCGTCGCTACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGGTTTTGATGAAGTACAGAAAGTTTGGCGTATCGTGCAGaaa                                >  2:553888/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                     ttGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGTCACGCAGCTCGTCGCTACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGGTTTTGATGAAGTACAGAAAGTTTGGAGTATCGTACAAAACGGCGTc                          >  1:412917/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                           cccTCTTCCTAAGTCTCTCCCCATAGGGTAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCTAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGTCACGCAGCTCGTCGCTACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGGTTTTGATGAAGTACAGAATGTTTGGCGTATCGTGCAGacgccgttctgcacg                    <  2:375174/301‑15 (MQ=255)
                                                                                                                                                                                                                                                                                ggcagttcccTCTCCCCTATGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGTCACGCAGCTCGTCGCTACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGGTTTTGATGAAGTACAGAATGTTTGGCGTATCGTGCAGAACGGCGTCTGCGCGGTTgg               >  2:164473/9‑301 (MQ=255)
                                                                                                                                                                                                                                                                                aCCCTGTTCCTCTCCCCATGGGGGAGAGGGGTCTGCCAGTTCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCTGTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTGGTCACGCAGCTCGTCGCTACCTGTCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGGCACCTACCGCTTTGAACTGACCGTTACCGGCGGTTTTGATGAAGTACAGAATGTTTGGCGTATCGTGCAGAACGGCGTCTGCGCGGTTgg               <  1:615794/301‑1 (MQ=255)
                                                                                                                                                                                                                                                                                   cgaaTCCTCTCCCCATAGGGGAGAGGTAACTGCCAGTGCGTTTTACAGGTTTTGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGTCACGCAGCTCGTCGCTACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGGTTTTGATGAAGTACAGAATGTTTGGCGTATCGTGCAGAACGGCGTCTGCGCGGTTGGTGc            <  1:465719/299‑1 (MQ=255)
                                                                                                                                                                                                                                                                                      tcccTCTCCCCTATGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGTCACGCAGCTCGTCGCTACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGGTTTTGATGAAGTACAGAATGTTTGGCGTATCGTGCAGAACGGCGTCAGCGCGGTTGGTGCCCa         >  2:607766/3‑301 (MQ=255)
                                                                                                                                                                                                                                                                                             ccccATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGTCACGCAGCTCGTCGCTACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGGTTTTGATGAAGTACAGAATGTTTGGCGTATCGTGCAGAACGGCGTCTGCGCGGTTGGTGCCCAGTTCCat  >  1:107781/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                                             ccccATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGTCACGCAGCTCGTCGCTACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGGTTTTGATGAAGTACAGAATGTTTGGCGTATCGTGCAGAACGGCGTCTGCGCGGTTGGTGCCCAGTTCCaa  >  2:45467/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                                        
GTGTGCCGCGGATCACTTCAATAAAGACCAGCGCGACGTGGTTGGCTATCCAACCTCCGAAGGTGCGTGCAAAACCTGCCAGCAATCCGATTACCAGACCGCCTGCCAGACCGAGGACCGAAATCCACAGGGTCATTTTGGCACCTTCAATCAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGTCACGCAGCTCGTCGCTACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGGTTTTGATGAAGTACAGAATGTTTGGCGTATCGTGCAGAACGGCGTCTGCGCGGTTGGTGCCCAGTTCCAT  >  NC_000913/846924‑847509

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: