Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,956,973 +A 7.1% coding (848/894 nt) ilvY ← transcriptional activator of ilvC; autorepressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,956,9731.A 7.1% 208.0 / 12.2 56coding (848/894 nt)ilvYtranscriptional activator of ilvC; autorepressor
Reads supporting (aligned to +/- strand):  ref base . (30/22);  new base A (2/2);  total (32/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.05e-01

CGCTTCCTCAACACGCTGGGTACGTACTGGAACATTTCTTTGTTCCACTATCGCAGCCATGGCACCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCT‑CATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAATCATCGGGTTACTGATTTTATTGCGTCGAAACCACAGTTCAATGCGGCGGCGTACCGGCCCCTGATCGGCCATAATAAACGGCACCGTTGACCAGTCCGG  >  NC_000913/3956675‑3957265
                                                                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                                                                    
cgcTTCCTCAACACGCTGGGTACGTACTGGAACATTTCTTTGTTCCACTATCGCAGCCATGGCACCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCT‑Ca                                                                                                                                                                                                                                                                                                    <  2:248465/301‑1 (MQ=255)
   ttCCTCAACACGCTGGGTACGTACTGGAACATTTCTTTGTTCCACTATCGCAGCCATGGCACCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCtacatg                                                                                                                                                                                                                                                                                                  <  2:228171/301‑6 (MQ=255)
       acAACACGTTGGGTACGTACTGGAACATTTCTTTGTTCCACTAGCGCAGCCATGGCCCCGACGACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCT‑CATGTAGcc                                                                                                                                                                                                                                                                                             <  1:166384/300‑1 (MQ=255)
               ttGGGTACGTACTGGAACATTTCTTTGTTCCACTATCGCAGCCATGGCACCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTACATG‑AGCCGCtaattg                                                                                                                                                                                                                                                                                     <  2:121617/300‑6 (MQ=255)
               gctctgccgttcgtgAACATTTTTTTGTGCTACTAACGCAGCAATGGCACCGACGACGGGCGCTGACTGGCGGCGTTCGAGCTTGGCGACCATGAACCGTGTGTCGACACCCGGCGGAATGAGCTGGGCGACGGGTGCCACGACGAAACGAATGACCCGGCGTTCAGGTTCTGTGTGGCGGGTGAGGGAGAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAGCGACATTTGTGGTTCGGCAAAATCGTCCAGAATGCCTCAATGAGCGGCT‑CATGTAGCCTCTTTTTc                                                                                                                                                                                                                                                                                     <  1:370810/287‑1 (MQ=255)
                            ggcaCATTTCTTTGTTCCACTATCGCAGCCATGGCACCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGAGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGGAAAATCTTCCAGAATGCCTCAATTAGCGGCT‑CATGAAGCCGCTTGTTcagaacggaagaga                                                                                                                                                                                                                                                                        >  2:371306/4‑288 (MQ=255)
                            ggAACATTTCTTTGTTCCACTATCGCAGCCATGGCACCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGCTTGCCACGACGAAACCAATAACCCGGCGTTCACGTTCTTTTTGGCGGGATAGGGAAAAATGCCTGATAGCGCTTCGCTTCTCAGGCCTACCCGCGCGACAACGCCATTTGTGGTTCGGCAAACTCTTCCAGACTGCCTCAATTAGCGACT‑CAACTAGCCCCTTTTTCTGCCCacacaccc                                                                                                                                                                                                                                                                        >  1:361887/1‑299 (MQ=255)
                               aCATTTCTTTGTTCCACTATCGCAGCCATGGCACCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTAACCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTACATTAGCGGCT‑CATGTAGCCGCTTTTTCTGCGCACACACGCCCa                                                                                                                                                                                                                                                                     >  2:634452/1‑301 (MQ=255)
                                  tttCTTTGTTCCACTGTCGCAGCCATGGCACCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCT‑CATGTAGCCGCTTTTTCTGCGCACACACGCCCAGcc                                                                                                                                                                                                                                                                  >  2:8891/1‑300 (MQ=255)
                                     ttttGTTTCACTATCGCAGCCATGGCACCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGTCGACCATGAACCGGATTTCGAACCCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTACATG‑AGCCGCtaattgaggcattctggaagattttgcc                                                                                                                                                                                                                                                               <  2:348455/300‑28 (MQ=255)
                                            ccACTATCGCAGCCATGGCACCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGATTCGCTTATCAGGCCTACCCGCGCGCCAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCT‑CATGTAGCCGCTTTTTCTGCGCACCAACGCCCAGCTCAAACGGCgc                                                                                                                                                                                                                                                        >  1:34665/1‑300 (MQ=255)
                                                tATCGCAGCCATGGCACCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCT‑CATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTtc                                                                                                                                                                                                                                                    <  2:535957/301‑1 (MQ=255)
                                                   cGCAGCCATGGCACCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCT‑CATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCa                                                                                                                                                                                                                                                 <  2:604820/301‑1 (MQ=255)
                                                            ggCACCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCT‑CATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTgcgc                                                                                                                                                                                                                                        >  2:281924/1‑301 (MQ=255)
                                                                ccGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCT‑CATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTa                                                                                                                                                                                                                                    <  2:577083/301‑1 (MQ=255)
                                                                         ggggcgTACTGGCGGCGTTGGTACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTGTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCT‑CATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAAtcatca                                                                                                                                                                                                                           <  1:106590/298‑1 (MQ=255)
                                                                               tACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCAGCAAAATCTTCCAGAATGCCTCAATTAGCGGCT‑CATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCATTTTCTCATCGCTGAGCTCTAAAATCATCACGCGGt                                                                                                                                                                                                                     >  2:248546/1‑301 (MQ=255)
                                                                                aCTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCT‑CATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGtt                                                                                                                                                                                                                    <  1:255252/301‑1 (MQ=255)
                                                                                aCTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCT‑CATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGAGGtt                                                                                                                                                                                                                    >  2:408219/1‑301 (MQ=255)
                                                                                            cGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCT‑CATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCCCCgaacc                                                                                                                                                                                                        <  2:34716/301‑5 (MQ=255)
                                                                                              aatttGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTTGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCT‑CATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCgg                                                                                                                                                                                                      <  1:633575/298‑1 (MQ=255)
                                                                                                                cGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGACAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCT‑CATGTAGCCGACTTTTCTGCGCACACACGCCAAGCCCAAACGGCGCTTTCTCATCGCTGCCCACTAAAATCATCACGCGGCTACCCACCAGTCAGGCGCCGTTTTccaccaacc                                                                                                                                                                                    >  1:23360/1‑298 (MQ=255)
                                                                                                                       cGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCT‑CATGTAGTCGCTTTTTCTGCGCCCACACCCCCAGCTCAACCGGCGTTTTCTCATACCTGCGCTCTAAAACAACCACGCAGTTACGCCCCGGTTCGGGGCTGTTTTCCAGCACCCCTTCCgg                                                                                                                                                                             >  1:344880/1‑301 (MQ=255)
                                                                                                                            cccGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCT‑CATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGcaaca                                                                                                                                                                        <  2:345343/301‑1 (MQ=255)
                                                                                                                               ggCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACCACGACATGTGTGCTTCGGCAAAATCTTCCAGACTACATCAATTAGCGGCT‑CATGTAGCCGCTTTTTATGCGCACACACGCCCAGCTCAAACAGCGATTTCTCATCGCTGCGCTCTAAAACAATCACGCAGAGACGCCCCTGTTCAGGTCTGTTTTACAGcaccacccaccgccaacaac                                                                                                                                                                     >  1:434567/1‑287 (MQ=255)
                                                                                                                                                  ctaTTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCT‑CATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGc                                                                                                                                                  <  2:169402/299‑1 (MQ=255)
                                                                                                                                                       gCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCT‑CATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTAccacca                                                                                                                                             >  2:307146/1‑301 (MQ=255)
                                                                                                                                                        ccACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCT‑CATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGccaccaa                                                                                                                                            >  2:52512/1‑300 (MQ=255)
                                                                                                                                                         cACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCT‑CATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGACACCCACCATc                                                                                                                                           >  2:538825/1‑301 (MQ=255)
                                                                                                                                                           cgacgaAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCT‑CATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACCCCACAGCCGACTGCCACCACCgc                                                                                                                                         >  1:76479/1‑300 (MQ=255)
                                                                                                                                                             acgaAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCT‑CATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATACCACGCCACAGCCGACTGccaccaccacat                                                                                                                                       >  1:548402/1‑295 (MQ=255)
                                                                                                                                                                aaaCCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCT‑CATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCCCCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACACTGCCACGCCACAGCCGAGTaccacccacccccacc                                                                                                                                    >  1:421319/1‑291 (MQ=255)
                                                                                                                                                                 aaCCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCT‑CATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACACCTTCCGGCAACAAAGCCACGCCACAGCCGAGTGCCACCACCGAAACCac                                                                                                                                   >  1:269780/1‑300 (MQ=255)
                                                                                                                                                                            gggggtgaggtttgttgtgggCGGGTTAGGAAAAAAGTCGTGGTTGCGGTTCGTTTATGTGTCTTACCCGCGCGACAACGTCATTGGTGGTTCGGCAAAATCGTCCCGAATGCCGCAGTTAGCGGCT‑CATGTGGCCGCGTTTGCTGCGCACACACGCCCAGCTCAAACGGCGTTTGCGCATCGCTGCGCTCTAAGATCAGCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACATTGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGcc                                                                                                                        <  1:334631/282‑1 (MQ=255)
                                                                                                                                                                                     ggTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCT‑CATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCAACAGCCGAGTGCCACCATCGAAACCATCGCTTAATGCCCACCAAccc                                                                                                               >  1:203694/1‑300 (MQ=255)
                                                                                                                                                                                             ttGGGGGGTTAGGGAAAAATGCCTGATAGCGCTGCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCT‑CATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTaa                                                                                                       <  1:281621/301‑1 (MQ=255)
                                                                                                                                                                                               ggCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCT‑CATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTaaaa                                                                                                     >  2:463161/1‑300 (MQ=255)
                                                                                                                                                                                                      tAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCT‑CATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTACTGCCCGCCAAACGAGGCGTAAATCAACggg                                                                                              >  1:274582/1‑301 (MQ=255)
                                                                                                                                                                                                        gggAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCT‑CATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGAAACCATCGCTTCATGCCCGCCAACCGTGGCGTAAATACACGGGtt                                                                                            >  2:304601/1‑301 (MQ=255)
                                                                                                                                                                                                          gAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCT‑CATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAATCATCGGGTTAc                                                                                          >  2:495479/1‑301 (MQ=255)
                                                                                                                                                                                                                     taTAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCT‑CATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAATCATCGGGTTACTGATTTTATTg                                                                               <  2:203937/300‑1 (MQ=255)
                                                                                                                                                                                                                                 tttATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCT‑CATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAATCATCGGGTTACTGATTTTATTGCGTCGAAACcac                                                                   <  2:549075/300‑1 (MQ=255)
                                                                                                                                                                                                                                        ggCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCT‑CATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCACACGGCGTTTTCTCATCGCTGCGCTCTAAACTCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACCGCCGAGTGCCACCATCGCAACCCTCGCCTCATGCCCGCCAAACGCGGCGTACAACATCCGGTTAACTAATTTACTCCCTCAACACCACAGATCaa                                                            >  1:560265/1‑301 (MQ=255)
                                                                                                                                                                                                                                          tctctctgcgcgACATCGTCTTTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCT‑CATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAATCATCGGGTTACTGATTTTATTGCGTCGAAACCACAGTTCAATg                                                          <  2:560979/294‑1 (MQ=255)
                                                                                                                                                                                                                                              cccGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCT‑CATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCCCCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAAACATCGGGTTACTGATTTTATTACGTCGAACACACAGTTCAATgcggg                                                      >  1:453461/1‑300 (MQ=255)
                                                                                                                                                                                                                                                 gcgtgACAACGTCATTTGTGTTTCGTCAAAATCTTCCAGAATGCCTCAATTAGCGGCT‑CATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAATCATCGGGTTACTGATTTTATTGCGTCGAAACCACAGTTCAATgcggcggc                                                   <  2:362371/301‑1 (MQ=255)
                                                                                                                                                                                                                                                    ctaCAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCT‑CATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAATCATCGGGTTACTGATTTTATTGCGTCGAAACCACAGTTCAATGCGGCGGCGTa                                                <  2:454025/299‑1 (MQ=255)
                                                                                                                                                                                                                                                              aTTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCT‑CATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAATCATCGGGTTACTGATTTTATTTCGTCGAAACCACAGTTCAATGCGGCGGCGTACCGGCCCCTg                                      >  2:406162/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                    ggTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCT‑CATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAACTCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCCCTTCCGGCACCAATGCAACGCCACAGCCGAGTGCCCACATCGATACCATCCATTCATGCCCCCCAACCGTAGCGTAAATCACCGAGTTACTGACTTTACTGCATCGAAAACACACTACAAAGAGGCTGCCTAACGGccacggctcgcc                                >  1:102860/1‑291 (MQ=255)
                                                                                                                                                                                                                                                                       tCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCT‑CATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCCCCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAATCATCGGGTTACTGATTTTATTGCGTCAAAACCACAGTTCAATGCGGCGGCTGACCGGCCCCttaaccgcccc                             >  1:335014/1‑291 (MQ=255)
                                                                                                                                                                                                                                                                        cGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCT‑CATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCCCCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCCTAAATCAACGGGTTACTGATTTTATTGCGTCGAAACCACCGTTCAAATGGGCGGCGTCCCGGccccccaaccgcccca                            >  1:334705/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                        cGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCT‑CATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAATCATCGGGTTACTGATTTTATTGCGTCGAAACCACAGTTCAATGCGGCGGCGTACCGGCCCCTGATCGGCCata                            <  2:274885/301‑1 (MQ=255)
                                                                                                                                                                                                                                                                               ttCTTCCAGAATGCCTCAATTAGCGGCT‑CATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAATCATCGGGTTACTGATTTTATTGCGTCGAAACCACAGTTCAATGCGGCGGCGTACCGGCCCCTGATCGGCCATAATAAACg                     <  2:270078/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                                 cTTCCAGAATGCCTCAATTAGCGGCT‑CATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAATCATCGGGTTACTGATTTTATTGCGTCGAAACCACAGTTCAATGCGGCGGCGTACCGGCCCCTGATCGGCCATAAAAAACGGc                   >  2:376124/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                                          ggCCTCAATGATCGGGT‑CTGGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAATCATCGGGTTACTGATTTTATTGCGTCGAAACCACAGTTCAATGCGGCGGCGTACCGGCCCCTGATCGGCCATAATAAACGGCACCGTTGAc          <  1:306742/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                                             cagaaaaaGCGGCTACATG‑AGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAATCATCGGGTTACTGATTTTATTGCGTCGAAACCACAGTTCAATGCGGCGGCGTACCGGCCCCTGATCGGCCATAATAAACGGCACCATTGACCaa       >  2:580845/8‑300 (MQ=255)
                                                                                                                                                                                                                                                                                                  aaaGCGGCTACATG‑AGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGTGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCAAGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAATCATCGGGTTACTGATTTTATTGCGTCGAAACCACAGTTCAAGGCGGAGGCGTACCGGCCCCTGATCGGCCATAAAAACCGGCACCCGTAACCAGccccg  >  1:266363/3‑299 (MQ=255)
                                                                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                                                                    
CGCTTCCTCAACACGCTGGGTACGTACTGGAACATTTCTTTGTTCCACTATCGCAGCCATGGCACCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCT‑CATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAATCATCGGGTTACTGATTTTATTGCGTCGAAACCACAGTTCAATGCGGCGGCGTACCGGCCCCTGATCGGCCATAATAAACGGCACCGTTGACCAGTCCGG  >  NC_000913/3956675‑3957265

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑