Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC	NC_000913	1,915,474	Δ12 bp	2.7%	coding (50‑61/699 nt)	proQ ←	RNA chaperone, putative ProP translation regulator

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 1915473	182 (1.210)	5 (0.030)	5/540	NT	2.7%	coding (62/699 nt)	proQ	RNA chaperone, putative ProP translation regulator
	?	NC_000913	1915486 =	176 (1.180)					coding (49/699 nt)	proQ	RNA chaperone, putative ProP translation regulator

GTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACGCGTTGCGCCGGGTTTAACACCGTAAAGATAACGCCAGCTCGAAGTGTAGAGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACAGTGGGGAA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/1915186‑1915473 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑aGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTCCTGATTACAACGGACGTAGCCAACAAGCGCAGGCATGAACAGGCGTCATTATAATGACGCTATCAGTAAATGCTACGTTATCCGTTGATTATC  >  NC_000913/1915486‑1915635                                                                                                                                                                                                                                                                                                                                                                                                                                                        GTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACGGCATGGGTTGCCGTCAAGATCGACACGCGTTGCGCCGGGTTTAACACCGTAAAGATAACGCCAGCTCGAAGTGTAGAGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTCCCCAGCAACCCGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGCCGCGCTTCCCCTTCCGCACTGCAACCGTGGGGAAGAACCGCCATTca                                                                                                                                           >  1:1146778/1‑299                    AACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACGGCATGGGTTGCCGTCAAGATCGACACGCGTTGCGCCGGGTTTAACACCGTAAAGATAACGCCAGCTCGAAGTGTAGAGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACAGTGGGGAAGAAACGCGATTACTTCTTTACTGCTATTCAA                                                                                                                         <  2:1146803/300‑1                                                                                                                 tGTTGGGCAGGGTTTAACAACGTAAAGTTAACGCCAGGTCGAAGTGAAGAGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTCCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACAGTGGGGAAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTCCTGATTACAACGGACGTAGCCAACAAGCGCAGGCATGAACAGGCGTCATTATAATGACGCTATCAGTA                           <  1:356337/300‑1                                                                                                                                    CCGTAAAGATAACGCCAGCTCGAAGTGTAGAGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACAGTGGGGAAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTCCTGATTACAACGGACGTAGCCAACAAGCGCAGGCATGAACAGGCGTCATTATAATGACG                                    >  2:297908/1‑273                                                                                                                                          AGATAACGCCAGCTCGAAGTGTAGAGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACAGTGGGGAAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTCCTGATTACAACGGACGTAGCCAACAAGCGCAGGCATGAACAGGCCTCATTATAATGCCGCTATCAGTAAATGCTACGTTATCCGTTGATTATt  >  2:1328641/1‑300                                                                                                                                                                                                                                                                                                                                                                                                                                                        GTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACGCGTTGCGCCGGGTTTAACACCGTAAAGATAACGCCAGCTCGAAGTGTAGAGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACAGTGGGGAA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/1915186‑1915473 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑aGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTCCTGATTACAACGGACGTAGCCAACAAGCGCAGGCATGAACAGGCGTCATTATAATGACGCTATCAGTAAATGCTACGTTATCCGTTGATTATC  >  NC_000913/1915486‑1915635

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.